`Cavalier` zoysiagrass plant
An asexually reproduced variety of perennial zoysiagrass with a unique combination of characters including an absence of leaf blade hairs, high turf quality, resistance to fall armyworm and a distinct DNA fingerprint.
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The present invention relates to a new and distinct asexually reproduced variety of perennial zoysiagrass (Zoysia matrella (L.)) Merr.
BACKGROUND OF THE INVENTIONThis invention relates to a new and distinct perennial zoysiagrass cultivar identified as `Cavalier` zoysiagrass (hereinafter referred to as `Cavalier`), that was tested as DALZ8507. `Cavalier` was discovered in a cultivated area that received mowing and traffic on Seoul National University campus, Suwon, South Korea. It was identified as a unique turfgrass clone growing among a diverse population of common zoysiagrasses native to the area.
For purposes of registration under the "International Convention for the Protection of New Varieties of Plants" (generally known by its French acronym as the UPOV Convention) and noting Section 1612 of the Manual of Plant Examining Procedure, it is proposed that the new variety of zoysiagrass of the present invention be named `Cavalier` Zoysiagrass.
BRIEF DESCRIPTIONS OF THE ILLUSTRATIONSFIG. 1 depicts an up-close view of the leaf blade and ligule of `Cavalier`.
FIG. 2 depicts entire leaf blades and ligule of `Cavalier`.
FIG. 3 is a DNA fingerprint of `Cavalier` in comparison with known varieties of zoysiagrass.
DETAILED DESCRIPTION OF THE PLANT`Cavalier` was characterized in greenhouse and field conditions. `Cavalier` is a unique variety of zoysiagrass (Zoysia matrella (L.) Merr that was discovered under cultivated conditions described above. `Cavalier` was asexually reproduced by cutting of stolons and rhizomes, rooting them in soil, and planting of the rooted material to provide planting stock for studying performance and for comparison of morphological characters after propagation. `Cavalier` has been propagated by sod, plugs, sprigs, and stolons. Seed reproduction with self-fertility is not common in the Zoysia sp. No seedling establishment from `Cavalier` has been noticed in either greenhouse or field studies.
`Cavalier` spreads primarily by stolons. It has an intermediate to rapid growth rate, and has intermediate to low water use requirements. `Cavalier` produces little thatch with an optimum mowing height of 3/8" to 2.5". `Cavalier` will generally cover in 10-12 months growing time when plug planted with 3".times.4" plugs planted on 12" centers or by sprigs.
`Cavalier` has both rhizome and stolon growth. The stolons of `Cavalier` have a mean internode length of 16.7 mm between the fourth to the fifth node, 18.4 mm between the second and third nodes, with a mean stolon width and diameter from 1.35 to 1.14 mm (Tables 1,2). The stolons of `Cavalier` root adventitiously at the nodes. Color notations of plant tissues were based on the Munsell Color Charts for Plant Tissues, Munsell Color, Baltimore, Md., 1977. Light quality, photoperiod, and general growth of the plants affect color notations. The internode stolon color of `Cavalier` stolons exposed to full sun is 5R 3/6.
Leaf blades of `Cavalier` are rolled in the bud, and are flat and stiff. The leaf blade length of `Cavalier` ranges from 58.9 to 60.7 mm and from 1.33 to 1.79 mm in width (Table 3,4,5), shorter and narrower than `El Toro` and `Meyer`. There are not any hairs on the abaxial/adaxial leaf surfaces of `Cavalier`. Measured under greenhouse conditions in January 1996, the genetic, adaxial leaf color of `Cavalier` is 2.5 GY 5/2 with `El Toro` having a leaf color of 2.5 GY 5/2, and `Meyer` having a color of 2.5 G 3/4. The ligule of `Cavalier` is a row of silky hairs, approximately 2.1mm in length on the longest hairs.
The flat leaf length of `Cavalier` is a mean of 2.66 cm when measured in a greenhouse, Dallas, Tex., January 1996. `Cavalier` has 2.5 GY 7/4 anthers and white colored stigmas, undistinguished in shade of color. The inflorescence of `Cavalier` is a terminal spike-like raceme, with spikelets on short pedicels. `Cavalier` has a mean culm length of 4.5 cm, a floral region of 13.3 mm, with a mean of 16.3 florets per raceme.
The chromosome number of `Cavalier` is 40.
`Cavalier` rated number one in the National Turf Evaluation Program (NTEP) trials over 4-yrs as tested at 24 locations ranging from California-Georgia and north to Nebraska and Colorado. `Cavalier` will find its primary utility in home lawns, golf course fairways and tee boxes and in parks and recreational areas (Table 6).
This test was planted into a sited shaded with live oak trees in 1 Sep,. 1992. Under tree shade with competition from trees for available moisture and nutrients, `Cavalier` had slightly more cover than `Belair` and `Meyer` (Table 7).
When `Cavalier` was compared with 59 other zoysiagrasses for salinity tolerance, it ranked number 22 in performance that was not different in injury rating for `Emerald` and superior to `Belair`, and `Meyer` (Table 8). When compared to other commercial varieties for root growth, `Cavalier` produced shorter average root depth, less root weight than `Emerald`, and clipping weights similar to `Emerald` (Table 9).
Nine zoysiagrass cultivars were tested for resistance to the fall armyworm (Spodoptera frugiperda) in laboratory tests (Table 10). Eggs of the fall armyworm (FAW) were obtained from the colony maintained at the USDA Laboratory at Tifton, Ga. Larvae were developed from the eggs, and feed with zoysiagrass tissue of the cultivars, with days to pupation and adult emergence of the FAW used as indicators of FAW resistance. When neonate larvae of FAW were confined on the zoysiagrass cultivars, less than 5% of the larvae survived beyond 4 days of `Cavalier`, `Emerald`, or `Belair`. Two cultivars, `Palisades` and `8516` were among the most susceptible with 57% and 55% survival respectively to 17 days and longer. No larvae were able to survive to 17 days on the cultivar `Cavalier`, indicating the resistance of `Cavalier` to FAW was in the form of larval mortality and an extended development period (Table 10).
`Cavalier` is distinguished from other zoysiagrass by its fine texture, long-narrow leaf, with low rhizome, but high stolon production. It has good to excellent salt tolerance, and good shade tolerance. `Cavalier` is resistant to the fall armyworm and the tropical sod webworm. `Cavalier` is intermediate in its growth and recovery rate. `Cavalier` has good to excellent winter hardiness and will persist in regions north to Kansas, Missouri and Southern Illinois.
TABLE 1
______________________________________
Internode length as measured between the second and third nodes,
internode diameter of the third internode, and node diameter of the
third node measured on zoysiagrass plants. Plants were grown in a
growth chamber with a 14-hour daylength, March 1995.
Internode Internode node
length diameter diameter
Genotype
mm--
mm--
mm--
______________________________________
`Cavalier` 18.4a* 1.35abc 1.35abc
`El Toro` 39.8a 1.45abc 1.45abc
`Meyer` 24.1a 1.99a 1.99a
______________________________________
*Analysis of variance by General Linear Models, with means followed by th
same letter not significantly different using Tukey's Studentized Range
(HSD), alpha = 0.05. Only selected means presented.
TABLE 2
______________________________________
Zoysiagrass internode lengths and stolen width measurements from the
fourth to the fifth nodes taken February 1988 on greenhouse grown
plants.
Internode Stolon
length width
Genotype
mm--
mm--
______________________________________
`Cavalier` 16.73bc* 1.14efg
`Emerald` 6.21e 1.02ghi
`Meyer` 16.47bc 1.53b
______________________________________
*Analysis of variance by General Linear Models, with means followed by th
same letter not significantly different using Tukey's Studentized Range
(HSD), alpha = 0.05. Only selected means presented.
TABLE 3
______________________________________
Zoysiagrass leaf measurements taken February 1988 on the fourth
youngest leaf from greenhouse grown plants.
Blade width Blade length
Genotype
mm--
mm--
______________________________________
`Cavalier` 1.79ij* 58.87e
`Emerald` 2.01fgh 30.56ff
`Meyer` 33.3b 82.33d
______________________________________
*Means followed by the same letter are not significantly different using
the WallerDuncan k ratio test (k ratio = 100). Only selected means
presented.
TABLE 4
______________________________________
Leaf blade width and length measured on the third youngest leaf of
zoysiagrasses. Plants were growing in a growth chamber with a
14-hour daylength, March 1995.
Blade width Blade length
Genotype
mm--
mm--
______________________________________
`Cavalier` 1.33d* 60.7cd
`El Toro` 3.26abc 68.3bcd
`Meyer` 2.59c 74.3bc
______________________________________
*Analysis of variance by General Linear Models, with means followed by th
same letter not significantly different using Tukey's Studentized Range
(HSD), alpha = 0.05. Only selected means presented.
TABLE 5
______________________________________
Zoysiagrass leaf sheath length measurements taken February 1988 on
the fourth youngest leaf from greenhouse grown plants.
Sheath length
Genotype
mm--
______________________________________
`Cavalier` 28.90ef*
`Emerald` 16.31ij
`Meyer` 30.68ed
______________________________________
*Means followed by the same letter are not significantly different using
the WallerDuncan k ratio test (k ratio = 100). Only selected means
presented.
TABLE 6
______________________________________
Mean turfgrass quality ranges of vegetative zoysiagrass cultivars grown
in the National Turfgrass Evaluation Program at 23 locations in the US
for 1992, 1993, and 1994.
Overall
Variety 1992 1993 1994 1995 4-Yr Avg.
______________________________________
`Cavalier` (DALZ8507)
5.95 6.23 5.89 5.99 5.93
`Marquis` (TC 2033)
5.85 6.10 6.11 5.96 5.91
`Sunburst` 5.83 5.91 5.81 5.87 5.85
`TC 5018` 5.80 5.81 5.92 5.70 5.81
`Emerald` 5.74 6.21 6.05 5.73 5.79
`Omni` (CD 2013)
5.56 6.13 6.06 5.96 5.73
`QT 2004` 5.63 6.01 5.86 5.57 5.63
`DALZ 8508` 5.59 6.06 5.74 5.60 5.60
`Palisades` (DALZ8514)
5.82 5.82 5.46 5.44 5.59
`Royal` (DALZ9006)
5.65 6.05 5.59 5.54 5.59
`Crowne` (DALZ8512)
5.80 5.76 5.50 5.45 5.55
`El Toro` 5.78 5.63 5.34 5.41 5.50
`CD 259-13` 5.30 5.53 5.74 5.49 5.40
`Meyer` 5.26 5.70 5.76 5.47 5.39
`QT 2047` 5.37 5.39 5.26 5.16 5.30
`Belair` 4.99 5.58 5.61 5.02 5.16
`DALZ 8516` 4.72 5.42 4.96 5.05 4.86
`Diamond` (DALZ8502)
4.40 5.03 4.58 4.36 4.41
`DALZ 8501` 4.88 4.31 3.99 4.05 4.27
`DALZ 8701` 4.23 4.10 3.71 3.58 3.85
LSD VALUE 0.22 0.20 0.20 0.20 0.17
______________________________________
To determine statistical differences among entries, subtract one entry's
mean from another entry's mean. Statistical difference occurs when this
value is larger than the corresponding LSD value (LSD 0.05).
In National Zoysiagrass Test 1991. Final Report 1992-95, NTEP No. 9615;
(Table 4); United States Department of Agriculture, Agricultural Research
Service, Beltsville Agricultural Research Center, Beltsville, MD 20705.
TABLE 7
______________________________________
Mean turf cover, as percentage of plot during turf, during winter
1993-1994 for the 1991 NTEP zoysia trial planted under 80% shade in
Dallas, TX.
Percentage Turf Cover
Variety 10 Nov 93 21 Dec 93
22 March 94
______________________________________
`Belair` 40.0 43.3 21.7
`Cavalier`
48.3 55.0 26.7
`Emerald`
41.7 51.7 26.7
`El Toro`
35.0 31.7 21.7
`Meyer` 33.3 38.3 23.3
MSD ns 14.5 10.2
______________________________________
MSD is the minimum significant difference between entry means for
comparison within column, and was based on the Duncan Waller kratio test
(kratio = 100). Only selected means presented.
In Morton, S. J., M. C. Engelke, and K. G. Porter. 1994. Performance of
three warmseason turfgrass genera cultured in shade III. Zoysia spp. In
Texas Turfgrass Research Report 1994. PR. 5242. p 27-29.
TABLE 8*
______________________________________
Average percent shoot salt injury (average of 20 rating dates) on
zoysiagrass entries in the 1991 NTEP.sup..dagger. Trials.
Entry Source Species.sup..dagger-dbl.
% Injury
______________________________________
`Diamond` NTEP 20 matrella 33ab.sup.
`El Toro` NTEP 13 japonica 38a-e
`Emerald` NTEP 10 jap x tenu
42a-h
`Cavalier` NTEP 17 matrella 42a-h
`Belair` NTEP 11 japonica 50f-k
`Meyer` NTEP 09 japonica 58lmn
`Korean Common`
NTEP 07* japonica 76pq
______________________________________
*Selected data set; complete data set includes 59 varieties and cultivars
.sup..dagger. NTEP = National Turfgrass Evaluation Program.
.sup..dagger-dbl. Species identity.
.sup..English Pound. Means followed by the same letter are not
significantly different, based on the WallerDuncan kratio ttest (kratio =
100)
In Marcum, K. B., M. C. Engelke, S. J. Morton and C. Dayton. 1994.
Salinity tolerances of selected bermudagrass and zoysiagrass genotypes. T
Turfgrass Res. 1993, Consolidated Prog. Rep. PR 5140: 105-107.
TABLE 9
______________________________________
Average mean root depth of zoysiagrasses grown in flexible tubes in
greenhouse studies, Dallas, TX.
Average Mean Total Root
Clipping
Root Depth Weight Weight
Variety
mm--
mg--
mg--
______________________________________
`Belair` 296 330 286
`Cavalier`
255 278 243
`El Toro`
356 473 391
`Emerald`
330 461 241
`Meyer` 333 411 466
MSD 79 161 267
______________________________________
*MSD = minimum significant difference for comparison of means within
columns based on the WallerDuncan kratio test where k = 100. Only selecte
means presented.
In Marcum, K. B., M. C. Engelke, S. J. Morton, and R. H. White. 1995.
Rooting charcteristics and associated drought resistance of zoysiagrasses
Agron. J. 87: 534-538.
TABLE 10
______________________________________
Resistance in zoysiagrass to 4-day old larvae of the fall armyworm;
survival, weight, and development time.
Cultivars
7 day larva Pupa Adult
and alive wt alive
wt alive
Genotypes
%.sup.1
mg.sup.2
%.sup.3
mg.sup.4
days.sup.5
%.sup.6
days.sup.7
______________________________________
`Cavalier`
0 -- 0 -- -- 0 --
`DALZ8501`
14.8 34.9a.sup.8
11.1 151.2ab
30.0a 11.1 40.0a
`K.Common`
14.8 41.2ab 11.1 136.1ab
30.3a 11.1 40.7a
`Belair`
25.9 75.5b 25.9 149.8ab
27.7ab
25.9 37.6abc
`El Toro`
33.3 58.7ab 26.7 171.1a
29.0a 26.7 39.0a
`Emerald`
37.0 52.1ab 37.0 137.5ab
30.5a 37.0 40.5a
`Meyer` 60.0 55.4ab 53.1 148.3ab
28.9a 53.3 38.8ab
`Palisades`
66.7 131.6c 66.7 122.7b
25.2b 66.7 35.3bc
`DALZ8516`
81.5 143.9c 74.1 156.7ab
25.1b 74.1 34.7c
______________________________________
.sup.1 Number of surviving 17day-old-larvae.
.sup.2 Mean larval weight taken after 17 days of feeding.
.sup.3 Number surviving to pupation.
.sup.4 Mean weight of pupa within 2 days of pupation.
.sup.5 Mean number of days to pupation.
.sup.6 Mean survival to adult.
.sup.7 Mean number of days from egg hatch to adult emergence.
.sup.8 Means in a column followed by the same letter are not significantl
different by WallerDuncan kratio t test (k = 100) (P = 0.05)
In Reinert, J. A., M. C. Engelke, S. J. Morton, P. S. Graff, and B. R.
Wiseman. 1994. Resistance in zoysiagrass (Zoysia spp) to the fall army
worm (Spodoptera frugiperda). TX turfgrass Research1992. Consolidated
Prog. Rep. PR 5248: 39-4
EXAMPLE 1
DNA Fingerprint Analysis
See Caetano-Annolles, B. J. Bassam and Peter M. Gresshoff, 1991, DNA Amplification fingerprinting using very short arbitrary oligonucleotide primers. Biotechnology. Vol. 9. Pp. 553-557.
The zoysiagrass amplification profiles were obtained using primer of sequence GCCCGCCC, and are compared to the standard `Meyer` (FIG. 2). Complex banding patterns and amplification fragment length polymorphisms were obtained in all cases. Results indicate bands fall into two categories, those that are common to the species, and those that in combination are characteristic of the cultivar (some identified by dots).
Statistical AnalysisStatistical analysis performed utilizes the method set forth in Steel and Torrie, 1960, Principles and Procedures of Statistics. Pages 1-481. McGraw-Hill Book Company, Inc. New York.
Claims
1. A new and distinct variety of zoysiagrass (Zoysia matrella (L.) Merr.) plant as described and illustrated herein, having the principle distinguishing characteristics of white stigmas, and absence of leaf blade hairs, high turf quality, resistance to fall armyworm and a distinct DNA fingerprint.
Type: Grant
Filed: Jul 8, 1997
Date of Patent: Feb 2, 1999
Assignee: The Texas A&M University System (College Station, TX)
Inventor: Milton Charles Engelke (Parker, TX)
Primary Examiner: Howard J. Locker
Assistant Examiner: Kent L. Bell
Law Firm: Fulbright & Jaworski LLP
Application Number: 8/890,959
International Classification: A01H 500;
