Hop variety named `Columbus`
A new and distinct variety of hop, Humulus lupulus L., named Columbus, has a superior yield of cones and a superior content of alpha acids in its resin. The new variety was cultivated as a result of a cross at a nursery near Prosser, Wash., United States, and has been asexually reproduced in and about Yakima, Wash., United States.
The field of the present invention is hops.
BACKGROUND OF THE INVENTIONOf conventional hop varieties, there are at least twenty named commercially grown hop varieties in the United States today, and some seventy-five to eighty varieties that are grown around the world. The varieties differ as to assorted physical features such as vine color and structure, as well as cone number, location, size and shape.
Commercial varieties of hop are female hops that exist as a perennial rootstock that produces an annual flush of above-ground, or aerial, vegetation. The perennial rootstock is comprised of a woody crown with a number of main roots and sub-roots that are used for water uptake, nutrient uptake, and winter starch storage. Over the winter, the woody crown supports many buds that develop into main vines (shoots) in the spring, emerge from the ground, and extend to produce the annual above-ground vegetation. In nature, with the onset of frosts in the fall, the above-ground portion of the plant "dies-back" to ground level, and only the perennial rootstock survives into the following year.
A main vine consists of a single flexible cylinder that produces pairs of leaves at nodes separated by variable distances along the length of the vine. The length of main vine between a pair of nodes is known as an internode. A bud is produced in the junction between leaf and vine, which junction is also known by the botanical term axil. Such a botanical term, as well as other botanical terms herein, are generally known to those of skill in the art, and can also be found in standard botanical texts such as Simon, E.W., et al., Lawson's Textbook of Botany--Revised, University Tutorial Press Ltd., London, England, 14th Ed. 1966. The bud grows and develops into a lateral, also known as a side-shoot or side-arm, with the same basic vegetative structure as the main vine. The buds on the laterals develop into groups of flowers that later mature into cones (strobiles). Because the laterals produce and support the cones of the hop, the structure of the laterals strongly influences the number of cones produced and hence the ultimate commercial yield of a variety.
The cones are the useful brewing commodity, and are referred to as "hops" in the commercial industry. Each cone is a group of flowers (inflorescence) consisting of sepals and petals (bracts and bracteoles) mounted on a central strig (or rachis). The resultant structure resembles a small pine cone or fir cone but is non-woody and green in color. In nature, the bracteoles support the seed of the plant.
Complete descriptions of the botanical structure of the hop plant are given in Burgess, "Hops--Botany, Cultivation and Utilization," Weed Crop Series, Interscience Publishers, Inc., New York, 1964 and Neve, "Hops," von Nostrand Reinhold, New York, 1991.
A trellis of poles and wire, commonly 18 feet in height and known as a "high trellis," supports strings that the main vines climb. The main vines grow to the top of the trellis, where they produce the laterals that support the groups of flowers that develop into the cones. Typically, the laterals bear cones only in the upper parts of the plant (i.e., closer to the main vine); the distance from the main vine varies from one variety to another. Under an alternative growing system, the "HopUnion Low Trellis Hop Growing System," the vines are grown on a trellis of single rows of 10 feet in height, like wine grapes (the 10 foot trellis is referred to herein as a "low trellis"). (Lewis, "The HopUnion U.S.A., Inc.--Low Trellis Hop Production System," Proc. Tech. Comm. of the IHGC of the XXXVIIIth International Hop Congress, Hereford, U.K. Published Rijksstation voor Landbouwtechniek, Merelbeke/Gent, Belgium, 17-37, 1990.) Under either system, when the cones are physiologically mature (ripe), the cones are harvested, then dried and compressed into bales for market, where the cones are typically used for the productuion of beverages such as beers, ales and related alcohol-containing liquids.
Different varieties of hops have differences in the chemical constituents of the lupulin of their cones. Accordingly, chemical analysis of such differences can be useful in distinguishing one hop variety from another. Such analysis is generally performed by high pressure liquid chromatography ("HPLC") of the soft resins along with gas liquid chromatography ("GLC") of the essential oils. Recently, Kenny developed a key for differentiation of hop varieties based on chemical analysis of the soft resins. (Kenny, "Identification of U.S. Grown Hop Cultivars by Hop Acid and Essential Oil Analyses," J. Amer. Soc. Brewing Chemists 48(1):3-8, 1990.) Further, Peacock and McCarty, as well as Kenny, have developed keys for the differentiation of hop varieties based on chemical analysis of the essential oils. (Peacock and McCarty, "Varietal Identification of Hops and Hop Pellets," Master Brewer Assoc. of the Americas Technical Quarterly 27:81-85, 1992; Kenny, supra.) These keys are based on gross differences in the amounts of, and ratios between, major constituents, which differences are large enough to overcome variation inherent in the analytical analysis of biological material.
SUMMARY OF THE INVENTIONThe present invention provides a new variety of hop (Humulus lupulus L.), which has been named `Columbus`. The new variety comprises a superior yield of cones and, as discussed below, a superior content of alpha acids in its soft resins. Columbus is particularly suited for the production of beverages such as beers, ales and related alcohol-containing liquids.
BRIEF DESCRIPTION OF THE DRAWINGSFIG. 1 depicts a section of Columbus vine near harvest.
FIG. 2 depicts a low trellis planting of Columbus.
FIG. 3 depicts early season growth of Columbus with an internode length of about 3 inches.
FIG. 4 depicts Columbus on a low trellis and shows the density of the cones in the canopy (cones per lateral node).
FIG. 5 depicts the physical appearance of Columbus cones.
DETAILED DESCRIPTION OF THE INVENTIONThe present invention provides a new variety of hop (Humulus lupulus L.), named `Columbus,` that has a superior yield of cones and a superior content of alpha acids in its soft resins. Columbus may be classed as a bitter hop (i.e., high in alpha acids content). Columbus was bred as the result of an open-pollination cross that was carried out in 1982 at a small hop nursery near Prosser, Wash. U.S.A, and was known by the identification number 9/17. Pursuant to growth, analysis, and testing, Columbus was identified and selected from numerous candidates arising from the cross. The exact parentage of Columbus is unknown. The breeding nursery that produced Columbus contained 20-30 female plants from which seed was collected en masse, and an approximately identical number of male plants were growing and flowering at the time that pollination occurred. Columbus is a diploid hop.
Set forth below are various characteristics that distinguish Columbus from conventional hop varieties. Columbus is initially distinguishable on the basis of its physical characteristics, including the location of its cones, the internode distance, number of cones per node, the yield of its cones, and the physical characteristics of the cones. Columbus is also distinguishable on the basis of its chemical characteristics, which are determined by chemical analyses of the soft resins and essential oils. Additionally, methods for asexual reproduction of Columbus are discussed.
I. Physical Characteristics of Columbus A. Location of ConesColumbus bears a high number of cones on its laterals down to a height of about 4 feet from the ground, when grown on a high trellis. A close-up Figure depicting in detail the vine structure of Columbus is presented as FIG. 1. Conversely, most conventional hop varieties on a high trellis do not bear significant numbers of cones below about 8 feet from the ground.
On a low trellis, Columbus produces significant numbers of cones down to ground level, and cones can even be found on untrained side-shoots running along the ground. (FIG. 2.) Conversely, most conventional hop varieties on a low trellis produce cones down to about 4 to 8 feet from the ground.
B. Internode DistanceThe internode distance on a main vine of any hop variety is important to the yield of cones because the shorter the internode length, the greater the number of yielding laterals for a given length of main vine. Columbus has a shorter than average mature internode length, about 8" to 12", with an early season internode distance of about 3". (FIG. 3.) Conversely, in conventional hop varieties such as Nugget, the mature internode length varies from 10" to 16", with an early season internode distance of about 8". There are also "dwarf hops" in which the mature internode length varies between 5" to 8".
C. Growth CharacteristicsAlthough the early season growth rate and length of time for hop vines to reach the top wire of a trellis vary widely due to factors such as climatic conditions and growth and harvest management, the early season growth of Columbus is generally faster than the most aroma hops (such as Tettnang, Perle, Centennial, Saaz, Spalt, Mt. Hood, Liberty, Hallertau, Hersbruck, and Fuggle), and also faster than the quicker-growing aroma hops such as Cascade and Willamette. Such aroma hops are generally first trained between May 7 and May 10, and reach the top wire between June 20 and June 30. The aroma hop Cluster and the bitter hops Galena and Chinook are typically trained about May 15 and reach the top wire from about June 20 to about June 30. The bitter hops Nugget, Olympic, Eroica, and Columbus are typically trained about May 25 and reach the top wire about June 27 to about July 7. Among this latter group, Columbus typically grows slower than the other hop varieties at first, but then accelerates its growth and can grow to the top faster than Nugget on occasion. In other words, Columbus is a late maturing hop, and has an early growth rate that is typical for bitter hops and for Cluster, but it is a slow starter.
D. HabitIn common with most other hop varieties, the main vines of Columbus are originally green in color but turn to dark red to purple as the season progresses. The main vines are ridged, and along the ridges are hooked hairs that adhere to the supporting trellis. A characteristic feature of Columbus vines, as discussed above, is the short internodes.
Although the lateral length of a hop variety can vary greatly due to factors such as climate and management practices, the laterals of Columbus are typically longer than those of aroma varieties, are typically similar in length to those of Galena and Nugget, and are typically shorter than Cluster, Chinook, Olympic and Eroica. A generalization of comparative lateral lengths is as follows:
TABLE 1
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Hop Variety Length of Lateral
______________________________________
Mt. Hood, Liberty, Saaz, Spalt, Perle,
1'-2'
Hallertau, Tettnang, Hersbruck, Fuggle,
Centennial
Cascade, Willamette 1.5'-2.5'
Columbus, Galena, Nugget
2.0'-3.0'
Cluster, Chinook, Olympic, Eroica
2.5'-3.5'
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Although most commercial hops have longer laterals in the head and much shorter laterals in the lower reaches of the vine. Columbus tends to have approximately even length laterals along the length of the vine. The sub-branches of the laterals develop as bunches of cones, and as set forth below, Columbus has an unusually high number of cones in each lateral bunch, typically greater than any other commercial variety.
The head of the vine is also subject to variation depending on such factors as climatic conditions and management practices, but Columbus can be generally grouped with Cascade. Willamette, Nugget and Galena as having a moderate head, although the head of Columbus is slightly smaller and neater than typical. The head of Columbus also appears more dense than most other varieties, apparently because more laterals are developed from an equivalent vine length due to the short internode distance discussed above.
In terms of overall vine morphology, Columbus tends to be stronger than the aroma varieties but slighter than the other bitter hops and Cluster. As noted above, the overall vine morphology also gives the appearance of being more dense than other commercial hop varieties.
E. Leaf MorphologyThe leaf morphology of Columbus is typical for most commercial hop varieties, and may be summarized as set forth in the table below.
TABLE 2
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Early Main Vine
Late Main Vine
Lateral
Character
Leaves Leaves Leaves
______________________________________
Number 2/node 2/node 2/node
Lobes cordate - 3 35 cordate - 3
Width 3-5" 4-7" 1-2"
Length 3-5" 4-7" 1-2"
Upper Surface
smooth smooth smooth
Texture
Lower Surface
fairly rough
rough slightly rough
Texture
Color med-dark green
dark green med-dark green
Margins lightly serrated
moderately serrated
lightly serrated
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The leaf color of Columbus is also similar to other commercial hop varieties, although in the early part of the growing season when Columbus is slower growing than other bitter hops and Cluster, Columbus has a darker leaf, while later in the season when Columbus is faster growing, the leaf of Columbus is similar to other hops in its class. The leaf of Columbus is typically not as dark as most of the slower growing aroma hops.
F. Number of Cones per NodeTypically, cones are found in high number clusters borne at nodes on a lateral that are close to the main vine ("basal lateral nodes"). Progressively fewer cones are found in the clusters located at nodes progressively farther away from the main vine. Ultimately, the lateral terminates in a tip bearing a single cone. Columbus yields a high number of cones at the basal lateral nodes as well as at nodes located farther away from the main vine. (FIG. 4.) The basal lateral nodes of Columbus have produced up to 42 cones. Conversely, conventional low yielding hop varieties typically bear clusters of about 8-16 cones at basal lateral nodes, while conventional high yielding varieties typically bear clusters of about 16-28 cones at basal lateral nodes.
G. Yield of ConesDue to the above discussed characteristics, Columbus provides an outstanding hop variety comprising the following: a plant bearing cones much lower down the main vine; a reduced internode length on the main vine, which results in a greater number of yielding laterals per given length of main vine; and, greater numbers of cones at each node, which results in greater numbers of cones on each of the yielding laterals. Accordingly, Columbus produces an outstanding yield of cones, whether grown on a high trellis or a low trellis.
Yield data for many hop varieties are compared with yield data for Columbus in Table 3. All hops were grown on a high trellis.
TABLE 3
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Washington State
HopUnion
Five-Year Average
1992 Average
Variety Yield (lb/acre)
Yield (lb/acre)
______________________________________
Low yielding varieties
Perle 1172 1366
Tettnang 970 1140
High yielding varieties
Cluster 2018 2407
Chinook 1938 2216
Nugget 1950
2829
Galena 1926
2502
Columbus -- 3290
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(Hop Growers of America, Inc., 1991-1992 Annual Statistical Report, 37th Annual HGA Convention, Portland, Oreg., 1993.)
Below in Table 4, yield data from low trellis hops grown at HopUnion farms for 1990 and 1992 are compared with experimental yield data for Columbus from 1991 and 1992.
TABLE 4
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Yield Data for Low Trellis Hops
1990 Average
1992 Average
Variety Yield (lb/acre)
Yield (lb/acre)
______________________________________
Perle 1557 1010
Tettnang 1062 800
Cascade 1415
1570
Chinook 1234
1017
Nugget 1712
1175
Galena 1800
1500
(Year: 1991)
Columbus 2832 3408
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First year plantings of hops typically yield significantly less than mature plantings of the same varieties. However, a 1992 first year planting of Columbus on a low trellis yielded, over an area of about 4.5 acres, an average of 2547 lb/acre. Columbus produces such yields with a growth habit that presents no problems for ease of harvesting.
H. Physical Characteristics of the ConesCones are typically described by size (large, medium, or small) and by degree of compactness (loose, medium, or tight). Size and compactness are indicative of the degree of compression (internode length) between the nodes of the central strig that subtends (supports) the bracts and bracteoles. Also, cones are often described in terms of weight, most often expressed in milligrams (mg). Light cones are usually between 120 and 160 mg in weight, heavy cones usually between 160-220 mg (kiln dry basis). Often the description includes a brief comment on general shape of the cone, such as round, ovoid, plump, etc.
Columbus cones are normally medium in length, typically about 1.25", medium to tight in compactness, and typically about 1" in diameter. Further, Columbus cones are typically quite rounded and plump, and weigh about 180-250 mg (kiln dry basis) at maturity. (FIG. 5.) Conversely, Chinook cones typically have bracts with the tips turned backwards (reflexed). Fuggle cones are typically square in cross-section. Nugget cones are typically compact, tight, and firm. Columbus cones are typically more dense than other commercial hop varieties, and hence typically heavier on a per cone basis. Columbus cones have little or no propensity to shatter when fresh or when properly dried.
I. Disease and Pest ResistanceThe susceptibility of Columbus to downy mildew (Pseudoperunospora humuli) is similar to Galena and Cluster, which are less resistant than most other commercial hop varieties. Columbus is apparently resistant to Verticillium wilt (Verticillium dahliae). Tests have indicated that Columbus can become infected with Prunus necrotic ringspot virus, hop latent virus and American hop latent virus, although it is also possible to free Columbus of these viruses by standard meristem culture techniques. Columbus has not shown any symptoms of infection when carrying these viruses, as is usual for most commercial hop varieties. Columbus shows no unusual tolerance or sensitivity to damson hop aphid (Phorodon humuli) and two spotted mite (Tetranychus urticae).
J. Quality ProfileBased upon sensory analyses of dried/baled leaf hop samples, Columbus has a quality profile that is similar to most other commercial hop varieties. Columbus has a mild, gentle aroma with no particular, peculiar character. However, Columbus is distinguishable from the commercial aroma varieties and Cluster by virtue of its higher resin content, which is indicated by visual inspection of the open cone and the much greater number of lupulin glands present in Columbus.
II. Chemical Characteristics of Columbus A. Chemical Analyses in GeneralChemical analysis of hops typically consists of analysis of the lupulin of the cones. The lupulin consists of the following chemical components and sub-components:
______________________________________
Soft resins alpha acids (humulones)
beta acids (lupulones)
Hard resins
Uncharacterized resins
Waxes
Tannins
Essential oils hydrocarbons
oxygenated compounds
sulfur compounds
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Of the above components, the soft resins and essential oils are of particular relevance to the differentiation of hop varieties. In regard to the soft resins, there are three analogues of the alpha acids, i.e., humulone, ad-humulone, and cohumulone, and there are also three analogues of the beta acids, i.e., lupulone, ad-lupulone, and colupulone.
Due to their chemical characteristics, some hop varieties are referred to as aromatic, or low alpha, hops and are known to contribute good hop flavor and aroma. Other hop varieties are high in bittering potential (i.e., high in alpha acids) and are referred to as bitter or high alpha hops. Columbus may be referred to as such a bitter or high alpha hop. Hop varieties in an intermediate varietal classification have medium levels of alpha acids and are referred to as dual-purpose hops.
B. Soft Resin CharacteristicsTable 5 lists the total amount of alpha and beta acids, the ratio of alpha acids to beta acids, and estimates of the relative proportions of cohumulone and colupulone for several high alpha hops. This data was determined by HPLC analysis according to standard methods well known to those having skill in the art. (See, e.g., American Society of Brewing Chemists, "Hops Method 12: Analysis of Hops, Hop Powders and Hop Pellets by High Pressure Liquid Chromatography," in "Methods of Analysis," The Society, St. Paul, Mn., 8th ed. 1991.) Table 5 also gives data on storage losses, which indicate the loss of alpha acids by oxidation. The data are expressed as ranges to account for inherent biological variation from sample to sample.
TABLE 5
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Parameter Chinook Nugget Galena
______________________________________
Alpha acids (% w/w)
12.0-14.0 12.0-14.0
11.0-13.0
cohumulone (%)
30-35
24-30
36-42
Beta acids (% w/w)
3.4-4.0
4.0-5.5
7.0-9.0
colupulone (%)
55-66
50-55
60-65
Alpha/beta ratio
3.5-4.0
2.5-3.0
1.5-2.0
Oil content (mls/100 g)
1.5-2.3
1.2-2.0
0.7-1.2
Loss of alpha acids (%)
35-45
20-35
20-30
6 months storage
room temperature
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Parameter Olympic Eroica Columbus
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Alpha acids (% w/w)
11.5-13.5 11.0-13.0
14.0-16.0
cohumulone (%)
38-43
30-35
Beta acids (% w/w)
4.0-6.0
4.0-5.5
4.5-5.5
colupulone (%)
57-62
67-70
55-60
Alpha/beta ratio
2.0-2.7
2.2-2.8
2.7-3.3
Oil content (mls/100 g)
1.4-2.0
0.5-1.3
1.5-2.2
Loss of alpha acids (%)
40-55
35-50
60-80
6 months storage
room temperature
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Table 5 indicates the following: (a) Columbus has a higher content of alpha acids than the other high alpha hops; (b) Columbus' cohumulone and colupulone contents are higher than Nugget, lower than Eroica and Galena, and similar to Chinook; (c) Columbus' beta acid content is higher than Chinook and lower than Galena; (d) Columbus' alpha/beta ratio is higher than Galena or Olympic but lower than Chinook; and (e) Columbus more rapidly loses its alpha acids in ambient storage than the other varieties.
Table 5 shows that Columbus has an alpha acids content well in excess of 10% w/w. This distinguishes Columbus from the aromatic hops. Examples of such aromatic hops are listed in Table 6.
TABLE 6
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Alpha acids content
Variety (% w/w of cone)
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Cascade 4.5-7.0
Cluster 5.5-8.5
Fuggle 4.0-5.5
Hallertau 3.5-5.5
Hersbruck 3.5-5.5
Liberty 3.0-5.0
Mount Hood 5.0-7.5
Perle 7.0-9.5
Saaz 3.0-4.5
Spalt 3.0-5.5
Tettnang 4.0-5.0
Willamette 4.0-6.0
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As discussed above, Kenny developed a key for identification of hop varieties based on the soft resin components. The application of the key of Kenny to Columbus is depicted in Table 7. At each decision point, certain varieties are eliminated according to a yes/no decision. The path to Columbus is shown by the double arrow. In the key depicted in Table 5, as well as in the other keys discussed herein, steps from the published key that did not eliminate any varieties in the present analysis have been deleted.
TABLE 7
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Major U.S. Variety Eliminations
______________________________________
1. Fresh alpha + beta > 15%
.fwdarw.
NO Cascade, Cluster,
and Fresh Alpha > 10% Fuggle, Hallertau,
Hersbruck, Liberty,
.arrow-down dbl.
YES Mt. Hood, Perle, Saaz,
(High alpha hops)
Spalt, Tettnang,
Willamette
2. Cohumulone > 40%
.fwdarw.
YES Eroica
.arrow-down dbl.
NO
3. Colupulone > 60%
.fwdarw.
YES Galena
.arrow-down dbl.
NO
4. Cohumulone < 30%
.fwdarw.
YES Nugget
.arrow-down dbl.
NO
5. Alpha/beta ratio > 3.0
.fwdarw.
YES Chinook (3.5-4.0)
.arrow-down dbl. .fwdarw.
NO Olympic (2.0-2.7)
Columbus(2.7-3.3)
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Table 7 relates to hops with a hop storage index, or HSI, well below 0.300, which indicates that the alpha and beta acids of the hops are fresh when analyzed. In Table 7, step 1 eliminates all the aromatic hops on the basis of their low content of alpha acids and low alpha plus beta acids. Step 2 eliminates Eroica on the basis of high cohumulone content. Step 3 eliminates Galena on the basis of high colupulone content. (Eroica is again eliminated at this step.) Step 4 eliminates Nugget on the basis of low cohumulone content. Step 5 eliminates Chinook on the basis of a high alpha/beta ratio, while Olympic is eliminated on the basis of a low alpha/beta ratio. Note, Chinook is also eliminated on the basis of its higher beta acid content.
Accordingly, Columbus is differentiated from all major U.S. varieties based on its soft resins content. Similarly, Columbus is distinguished from the minor U.S. hop varieties, except Aquila, Banner, and Centennial, based on soft resins content. However, Columbus is distinguished from Aquila, Banner, and Centennial by its essential oils content, as discussed below.
C. Essential Oils CharacteristicsDetailed analysis of the essential oils allows a further differentiation of Columbus from other hops. Table 8 further distinguishes some of the varieties discussed above in Table 7, while Table 9 distinguishes varieties not distinguished above.
Extraction and analysis of essential oils are performed according to methods well known to those having skill in the art. Preferably, the essential oils are extracted by steam distilling previously dried hops for about four hours (with the steam of the distillation condensed using a cold-finger condenser). The resultant essential oils are then examined by GLC analysis.
GLC analysis for various hops are presented as Appendices 1 and 2 hereto as follows:
Appendix 1: Detailed essential oil analysis results of many of the world's hop varieties including all U.S. grown varieties except Mount Hood. Columbus is the final entry.
Appendix 2: Detailed GLC traces and listings of constituent peaks from essential oils analysis. Columbus is compared with the other U.S. high alpha hops, Galena, Chinook, and Nugget.
As with the soft resins, keys can be used to differentiate by essential oils content. Only United States varietal differentiations are depicted below in Tables 8 and 9, but non-United States varietal eliminations are listed in Peacock and McCarty, supra, and can be readily utilized by those having skill in the art to distinguish between those varieties and Columbus.
TABLE 8
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Key of Kenny
Major U.S. variety eliminations
______________________________________
1. .beta.-Farnesene content > 1%
.fwdarw.
YES Cascade, Fuggle,
.arrow-down dbl.
NO Saaz, Spalt
Tettnang, Willamette
2. Humulene/ .fwdarw.
NO Eroica, Olympic
.beta.-Caryophyllene ratio > 1.5
.arrow-down dbl.
YES
3. Presence of 2-Tridecanone/
.fwdarw.
NO Galena
presence of a peak @ (*see note in text)
34.38 > 1.0%
.arrow-down dbl.
NO
4. .beta.-Caryophyllene/
.fwdarw.
YES Cluster, Nugget
.varies.-copaene > 20
.arrow-down dbl.
NO
Chinook Columbus
(12.1) (13.4)
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Table 8 was prepared using the data provided in Appendix 1. As shown in Table 8, step 1 eliminates the high .beta.-farnesene hops, i.e., Cascade, Fuggle, Saaz, Spalt, Tettnang, and Willamette. Step 2 eliminates the low humulene varieties, i.e., Eroica and Olympic. Step 3 eliminates Galena because Columbus is unique for having no 2-tridecanone, and Columbus lacks a large peak found in Galena at 34.38 minutes (the peak is greater than 1% of the measured components of Galena). Step 4 eliminates both Cluster and Nugget. The remaining varieties are Columbus and Chinook. As shown in Table 7, Columbus is differentiated from Chinook by Columbus' lower alpha/beta ratio. In regard to some other varieties, Perle is eliminated by virtue of its low alpha acids content, while Columbus is distinguishable from Hersbruck, Hallertau, Liberty, and Mount Hood because Columbus has a much higher alpha acids content.
TABLE 9
______________________________________
Key of Peacock and McCarty (1992)
Major U.S. variety eliminations
______________________________________
Columbus
.arrow-down dbl.
1. Humulene present
.fwdarw.
YES Aquila, Eroica
.arrow-down dbl.
YES
2. .beta.-Farnesene >
.fwdarw.
YES Cascade, Fuggle
5% Humulene Saaz, Spalt,
Tettnang, Willamette
.arrow-down dbl.
NO
3. Humulene/Selinene > 20
.fwdarw.
YES Banner, Hallertau,
Liberty, Mt. Hood,
Perle
.arrow-down dbl.
NO
4. Contains Eudesmol
.fwdarw.
YES Chinook, Hersbruck
.arrow-down dbl.
NO
5. Cohumulone < 38%
.fwdarw.
NO Cluster, Galena
.arrow-down dbl.
YES
Columbus, Nugget,
Olympic
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Table 9 depicts the key of Peacock and McCarty, which differentiates Aquila, Banner, and Centennial. The data from Appendix 1 is used for all steps except step 4, for which the data in Appendix 2 (which quantifies eusdesmol) is necessary. In Table 9, Step 1 eliminates Aquila and Eroica. Step 2 eliminates the six high .beta.-farnesene hops. Step 3 eliminates Perle, Banner, Hallertau, Liberty, and Mount Hood. Step 4 eliminates Hersbruck and Chinook. Step 5 eliminates Cluster and Galena. Table 7 does not distinguish Columbus from Nugget and Olympic. However, as discussed above, Nugget and Olympic are distinguished from Columbus by each of Table 7 and Table 8.
Finally, the variety Centennial has not yet been distinguished. The data in Appendix 1 show that Centennial has a .beta.-caryophyllene level of 2.649, while Columbus has a .beta.-caryophyllene level of 10.236. Therefore, the varieties are distinguished because they are at virtually opposite ends of the scale for .beta.-caryophyllene.
III. Asexual Reproduction (Propagation) of the PlantColumbus is asexually reproduced according to methods well known to those having skill in the art. Such methods generally comprise one of three procedures: (a) taking rootstock cuttings known as "roots" or "strap cuts;" (b) converting vine material into woody root-like material in the field and dividing this material into "layered cuttings;" or (c) the use of softwood cuttings, which are also known as "mist cuttings," of green vegetative or shoot material, usually under greenhouse conditions. Further, traditional methods can be applied to make a cell tissue culture from Columbus. Such a cell tissue culture can be stored for extended periods, and gives rise to clones (i.e., asexual progeny) of Columbus. Columbus has been asexually reproduced on ranches associated with HopUnion USA, Inc., located in and about Yakima, Wash. U.S.A.
A. Asexual Reproduction by Taking Rootstock CuttingsIn the field, when main vines die back in the fall, their bases turn woody and swell with the deposition of starch reserves. These bases produce buds at the sites where the main vine nodes were previously located. The bases, usually 4"-8" long and between one-half and two inches thick, are removed by cutting with a sharp knife; other buds on the rootstock produce re-growth in the spring. The detached vine bases are then planted singly or in multiples, in pots or in the field.
Such a procedure typically provides up to a 15-fold increase over the starting number of plants. The increase may be lower where multiple rootstocks (up to 6-8) are to be planted at a single site, to form a single new composite plant.
B. Asexual Reproduction by Layered CuttingsIn the field, main vines are grown up a string, typically to a pre-determined height of about 10 feet. The string is then lowered and the newly grown main vines laid along the ground (for example, for about 8 feet). The string is then re-hung so that a portion of the main vines can renew vertical growth, but in a new location. Some main vines are left laying along the ground and are buried with soil, where the absence of light on the buried, green main vine stimulates it to turn woody, form buds at the nodes and swell with starch deposits. When the unburied portion of the vine has died back, the buried, now woody, portion is dug up and cut up into numerous layered cuttings, which are then replanted to give new plants. Such a procedure provides up to about a 50-fold increase over the starting number of plants.
In an alternative embodiment, new plants are placed at the bottom of deep, open trenches, preferably in a nursery. As the plants grow, soil is periodically pushed into the trench around the vines, thereby yielding layered cuttings in a vertical plane.
C. Asexual Reproduction by Softwood (Mist) CuttingsActively growing main vines of a plant are cut into sections comprising a half-node, a single node, or two nodes. Main vines for this purpose are preferably grown in a greenhouse, where growing conditions can be maximized. The cuttings are planted and stuck in a suitable medium, preferably with bottom heat and an intermittent water mist, further preferably under glass. The cuttings produce a rooted plant with new main vine growth about two to three weeks after removal from the original plant. These rooted softwood cuttings can be further grown in pots, in a nursery, or even in the final new plantation. Using such a procedure, it is possible to achieve up to about a 100-fold increase over the starting number of plants.
APPENDIX 1
______________________________________
Detailed GLC analysis of assorted hop varieties.
Analytical Conditions: see Kenny (1990), supra.
OIL AREA PERCENTS OF COMMERCIAL HOPS
______________________________________
GC/ TENTATIVE IDENTI-
FID CMPD. FICATION BY GC./ 21223 21053
RT. # MASS SPECTROSCOPY
AQUILA AURORA
______________________________________
6.42 9 A-PINENE 0.105 0.112
7.46 14 ISOBUTYL ISOBUTYRATE
0.000 0.326
8.10 16 8-PINENE 0.898 0.817
9.11 18 MYRCENE 62.462 58.731
9.77 22 2-METHYL-BUTYL 0.171 0.159
PROPANOATE
9.90 23 ISOAMYL ISOBUTYRATE
0.000 0.076
9.96 24 2-METHYL-BUTYL 0.370 0.754
ISOBUTARATE
10.28
26 L-LIMONENE 0.243 0.228
10.62
27 B-PHELLANDRENE? 0.262 0.234
C10H15
11.59
32 trans-OCIMENE 1.542 0.207
12.45
35 2-METHYL-BUTYL 0.000 0.000
2-METHYL-BUTYRATE
12.68
36 METHYL HEPTANOATE
0.445 0.656
12.91
40 ISOAMYL ISOVALERATE
0.000 0.000
14.31
43 METHYL 4-METHYL- 0.069 0.160
HEXA-2-ENOATE
14.39
45 METHYL 6-METHYL- 0.230 0.264
HEPTANOATE
15.98
51 METHYL OCTANOATE 0.317 0.506
16.26
55 NONANAL 0.048 0.083
19.41
73 METHYL NONANOATE 0.172 0.343
19.56
75 2-DECANONE, 0.139 0.379
A-YLANGENE
19.93
77 A-COPAENE 0.121 0.161
21.00
83 LINALOOL 0.368 0.997
21.31
88 A-GURJUNENE 0.000 0.000
21.42
89 7-METHYL-2-DECANONE
0.062 0.218
22.56
93 C15H24 CALARENE? 0.000 0.000
22.72
95 A-BERGAMOTENE, 0.239 0.408
METHYL DECANOATE
23.01
96 2-UNDECANONE 0.448 1.402
23.10
97 2-DECANOL 0.000 0.160
23.44
99 8-CARYOPHYLLENE 4.104 4.403
23.84
102 METHYL DEC-4-ENOATE
1.608 1.286
24.03
103 MW168 2-OL? 0.000 0.181
ME 43,81,110,108
25.18
114 B-FARNESENE 0.000 4.371
26.10
122 HUMULENE 1.193 14.141
26.16
123 METHYL DEC-4,8- 1.462 0.310
DIENOATE
26.46
129 WILEY# 123998 MW 204
0.341 0.635
C15H24
26.59
131 2-DODECANONE 0.311 0.000
27.32
138 A-FARNESENE OR 0.270 0.132
ZINGIBERENE
27.53
139 CAS# 030021-74-0 0.000 0.000
27.75
143 A-MUUROLENE 0.114 0.182
27.90
144 B-SELINENE 2.421 0.109
28.04
145 A-SELINENE 2.745 0.130
28.34
147 METHYL 0.266 0.308
?-UNDECENOATE
29.16
154 D-CADINENE 0.531 0.696
29.39
155 G-CADINENE 0.191 0.381
30.34
164 CAS# 016728-99-7 OR
3.659 0.184
G-SELINENE
30.73
166 A-MUUROLINE 0.000 0.000
31.15
170 2-TRIDECANONE 1.620 1.080
31.36
171 GERANYL PROPANOATE
0.275 0.000
31.46
172 MW 212 METHYL 0.000 0.000
?-DODECENOATE
32.28
177 trans-GERANIOL 0.264 0.000
32.54
179 CAS# 000483-77-2 1.173 0.000
(CALAMENENE ?)
34.38
187 METHYL 3,6- 0.109 0.000
DODECDIENOATE ME 79
36.42
196 MW 210 ME 43,54,82
0.122 0.282
39.11
211 CARYOPHYLLENE OXIDE
0.121 0.083
40.23
221 HUMULENE 0.000 0.530
MONO-EPOXIDE I
40.28
223 MW222 ME 43,82 0.177 0.000
41.07
225 HUMULENE 0.000 0.199
MONO-EPOXIDE II
41.55
228 HUMULENE 0.000 0.000
MONO-EPOXIDE III
41.88
231 MW 222 ME 43,79,80
0.598 0.950
43.88
242 MW 220 ME 43,79,91
0.170 0.262
44.73
246 T-CADINOL 0.214 0.000
46.08
255 T-MUUROLOL 0.000 0.000
ME 95,121,204
47.50
262 MW 220 ME 41,67,109
0.000 0.000
DIEPOXIDE?
48.69
268 FARNESOL 0.407 0.000
______________________________________
GC/ TENTATIVE IDENTI-
FID CMPD. FICATION BY GC./ 21080 21287
RT. # MASS SPECTROSCOPY
BACKA BANNER
______________________________________
6.42 9 A-PINENE 0.000 0.110
7.46 14 ISOBUTYL ISOBUTYRATE
0.000 0.129
8.10 16 8-PINENE 0.275 0.842
9.11 18 MYRCENE 15.376 65.305
9.77 22 2-METHYL-BUTYL 0.075 0.391
PROPANOATE
9.90 23 ISOAMYL ISOBUTYRATE
0.000 0.173
9.96 24 2-METHYL-BUTYL 0.000 0.988
ISOBUTARATE
10.28
26 L-LIMONENE 0.108 0.243
10.62
27 B-PHELLANDRENE? 0.000 0.273
C10H15
11.59
32 trans-OCIMENE 0.000 0.681
12.45
35 2-METHYL-BUTYL 0.000 0.068
2-METHYL-BUTYRATE
12.68
36 METHYL HEPTANOATE
0.281 0.381
12.91
40 ISOAMYL ISOVALERATE
0.000 0.000
14.31
43 METHYL 4-METHYL- 0.298 0.093
HEXA-2-ENOATE
14.39
45 METHYL 6-METHYL- 0.000 0.413
HEPTANOATE
15.98
51 METHYL OCTANOATE 0.090 0.269
16.26
55 NONANAL 0.939 0.037
19.41
73 METHYL NONANOATE 0.075 0.188
19.56
75 2-DECANONE, 0.497 0.051
A-YLANGENE
19.93
77 A-COPAENE 0.420 0.173
21.00
83 LINALOOL 0.461 0.583
21.31
88 A-GURJUNENE 0.164 0.166
21.42
89 7-METHYL-2-DECANONE
0.384 0.000
22.56
93 C15H24 CALARENE? 0.382 0.069
22.72
95 A-BERGAMOTENE, 0.000 0.243
METHYL DECANOATE
23.01
96 2-UNDECANONE 1.442 0.046
23.10
97 2-DECANOL 0.685 0.173
23.44
99 8-CARYOPHYLLENE 10.792 5.478
23.84
102 METHYL DEC-4-ENOATE
0.588 0.910
24.03
103 MW168 2-OL? 0.394 0.000
ME 43,81,110,108
25.18
114 B-FARNESENE 0.439 0.000
26.10
122 HUMULENE 37.338 13.256
26.16
123 METHYL DEC-4,8- 0.000 0.157
DIENOATE
26.46
129 WILEY# 123998 MW 204
1.342 0.475
C15H24
26.59
131 2-DODECANONE 0.000 0.000
27.32
138 A-FARNESENE OR 0.114 0.134
ZINGIBERENE
27.53
139 CAS# 030021-74-0 0.182 0.094
27.75
143 A-MUUROLENE 0.314 0.173
27.90
144 B-SELINENE 0.391 0.126
28.04
145 A-SELINENE 0.429 0.140
28.34
147 METHYL 0.000 0.000
?-UNDECENOATE
29.16
154 D-CADINENE 1.688 0.873
29.39
155 G-CADINENE 1.075 0.478
30.34
164 CAS# 016728-99-7 OR
0.263 0.126
G-SELINENE
30.73
166 A-MUUROLINE 0.201 0.100
31.15
170 2-TRIDECANONE 0.871 1.945
31.36
171 GERANYL PROPANOATE
0.000 0.269
31.46
172 MW 212 METHYL 0.000 0.081
?-DODECENOATE
32.28
177 trans-GERANIOL 0.000 0.000
32.54
179 CAS# 000483-77-2 0.359 0.082
(CALAMENENE ?)
34.38
187 METHYL 3,6- 0.000 0.484
DODECDIENOATE ME 79
36.42
196 MW 210 ME 43,54,82
0.236 0.137
39.11
211 CARYOPHYLLENE OXIDE
2.753 0.097
40.23
221 HUMULENE 0.173 0.048
MONO-EPOXIDE I
40.28
223 MW222 ME 43,82 1.201 0.113
41.07
225 HUMULENE 9.016 0.176
MONO-EPOXIDE II
41.55
228 HUMULENE 0.124 0.000
MONO-EPOXIDE III
41.88
231 MW 222 ME 43,79,80
1.910 0.213
43.88
242 MW 220 ME 43,79,91
0.500 0.113
44.73
246 T-CADINOL 0.403 0.070
46.08
255 T-MUUROLOL 0.209 0.040
ME 95,121,204
47.50
262 MW 220 ME 41,67,109
0.333 0.000
DIEPOXIDE?
48.69
268 FARNESOL 0.000 0.084
______________________________________
GC/ TENTATIVE IDENTI-
21116
FID CMPD. FICATION BY GC./ BREWERS 64100
RT. # MASS SPECTROSCOPY
GOLD BULLION
______________________________________
6.42 9 A-PINENE 0.117 0.110
7.46 14 ISOBUTYL ISOBUTYRATE
0.169 0.251
8.10 16 8-PINENE 0.988 0.934
9.11 18 MYRCENE 69.050 67.113
9.77 22 2-METHYL-BUTYL 0.344 0.469
PROPANOATE
9.90 23 ISOAMYL ISOBUTYRATE
0.068 0.153
9.96 24 2-METHYL-BUTYL 1.267 1.053
ISOBUTARATE
10.28
26 L-LIMONENE 0.260 0.254
10.62
27 B-PHELLANDRENE? 0.328 0.324
C10H15
11.59
32 trans-OCIMENE 0.549 0.441
12.45
35 2-METHYL-BUTYL 0.096 0.045
2-METHYL-BUTYRATE
12.68
36 METHYL HEPTANOATE
0.298 0.543
12.91
40 ISOAMYL ISOVALERATE
0.000 0.038
14.31
43 METHYL 4-METHYL- 0.000 0.089
HEXA-2-ENOATE
14.39
45 METHYL 6-METHYL- 0.105 0.205
HEPTANOATE
15.98
51 METHYL OCTANOATE 0.206 0.174
16.26
55 NONANAL 0.049 0.040
19.41
73 METHYL NONANOATE 0.105 0.208
19.56
75 2-DECANONE, 0.040 0.070
A-YLANGENE
19.93
77 A-COPAENE 0.166 0.149
21.00
83 LINALOOL 0.461 0.441
21.31
88 A-GURJUNENE 0.039 0.070
21.42
89 7-METHYL-2-DECANONE
0.000 0.000
22.56
93 C15H24 CALARENE? 0.085 0.064
22.72
95 A-BERGAMOTENE, 0.105 0.154
METHYL DECANOATE
23.01
96 2-UNDECANONE 0.000 0.119
23.10
97 2-DECANOL 0.179 0.156
23.44
99 8-CARYOPHYLLENE 6.305 6.054
23.84
102 METHYL DEC-4-ENOATE
0.566 0.837
24.03
103 MW168 2-OL? 0.000 0.044
ME 43,81,110,108
25.18
114 B-FARNESENE 0.000 0.000
26.10
122 HUMULENE 10.326 10.525
26.16
123 METHYL DEC-4,8- 1.153 0.252
DIENOATE
26.46
129 WILEY# 123998 MW 204
0.433 0.396
C15H24
26.59
131 2-DODECANONE 0.000 0.047
27.32
138 A-FARNESENE OR 0.177 0.169
ZINGIBERENE
27.53
139 CAS# 030021-74-0 0.104 0.084
27.75
143 A-MUUROLENE 0.172 0.135
27.90
144 B-SELINENE 0.378 0.433
28.04
145 A-SELINENE 0.410 0.495
28.34
147 METHYL 0.000 0.000
?-UNDECENOATE
29.16
154 D-CADINENE 0.864 0.790
29.39
155 G-CADINENE 0.443 0.387
30.34
164 CAS# 016728-99-7 OR
0.080 0.084
G-SELINENE
30.73
166 A-MUUROLINE 0.091 0.083
31.15
170 2-TRIDECANONE 0.348 0.802
31.36
171 GERANYL PROPANOATE
0.257 0.493
31.46
172 MW 212 METHYL 0.000 0.000
?-DODECENOATE
32.28
177 trans-GERANIOL 1.003 0.060
32.54
179 CAS# 000483-77-2 0.000 0.051
(CALAMENENE ?)
34.38
187 METHYL 3,6- 0.000 0.126
DODECDIENOATE ME 79
36.42
196 MW 210 ME 43,54,82
0.000 0.267
39.11
211 CARYOPHYLLENE OXIDE
0.082 0.084
40.23
221 HUMULENE 0.097 0.227
MONO-EPOXIDE I
40.28
223 MW222 ME 43,82 0.047 0.107
41.07
225 HUMULENE 0.115 0.131
MONO-EPOXIDE II
41.55
228 HUMULENE 0.057 0.000
MONO-EPOXIDE III
41.88
231 MW 222 ME 43,79,80
0.073 0.469
43.88
242 MW 220 ME 43,79,91
0.000 0.265
44.73
246 T-CADINOL 0.078 0.073
46.08
255 T-MUUROLOL 0.046 0.044
ME 95,121,204
47.50
262 MW 220 ME 41,67,109
0.000 0.000
DIEPOXIDE?
48.69
268 FARNESOL 0.586 0.472
______________________________________
GC/ TENTATIVE IDENTI- 21507
FID CMPD. FICATION BY GC./ 56013 CENTE-
RT. # MASS SPECTROSCOPY
CASCADE NNIAL
______________________________________
6.42 9 A-PINENE 0.098 0.155
7.46 14 ISOBUTYL ISOBUTYRATE
0.110 0.127
8.10 16 8-PINENE 0.933 1.125
9.11 18 MYRCENE 68.051 77.911
9.77 22 2-METHYL-BUTYL 0.260 0.372
PROPANOATE
9.90 23 ISOAMYL ISOBUTYRATE
0.096 0.234
9.96 24 2-METHYL-BUTYL 0.527 0.911
ISOBUTARATE
10.28
26 L-LIMONENE 0.233 0.324
10.62
27 B-PHELLANDRENE? 0.337 0.358
C10H15
11.59
32 trans-OCIMENE 0.132 0.080
12.45
35 2-METHYL-BUTYL 0.000 0.094
2-METHYL-BUTYRATE
12.68
36 METHYL HEPTANOATE
0.210 0.333
12.91
40 ISOAMYL ISOVALERATE
0.043 0.126
14.31
43 METHYL 4-METHYL- 0.055 0.114
HEXA-2-ENOATE
14.39
45 METHYL 6-METHYL- 0.091 0.168
HEPTANOATE
15.98
51 METHYL OCTANOATE 0.093 0.193
16.26
55 NONANAL 0.051 0.000
19.41
73 METHYL NONANOATE 0.072 0.101
19.56
75 2-DECANONE, 0.044 0.000
A-YLANGENE
19.93
77 A-COPAENE 0.118 0.088
21.00
83 LINALOOL 0.312 0.470
21.31
88 A-GURJUNENE 0.000 0.054
21.42
89 7-METHYL-2-DECANONE
0.000 0.000
22.56
93 C15H24 CALARENE? 0.070 0.000
22.72
95 A-BERGAMOTENE, 0.275 0.050
METHYL DECANOATE
23.01
96 2-UNDECANONE 0.110 0.000
23.10
97 2-DECANOL 0.175 0.009
23.44
99 8-CARYOPHYLLENE 4.059 4.649
23.84
102 METHYL DEC-4-ENOATE
0.333 0.809
24.03
103 MW168 2-OL? 0.000 0.000
ME 43,81,110,108
25.18
114 B-FARNESENE 5.285 0.000
26.10
122 HUMULENE 10.063 5.699
26.16
123 METHYL DEC-4,8- 0.219 2.229
DIENOATE
26.46
129 WILEY# 123998 MW 204
0.325 0.239
C15H24
26.59
131 2-DODECANONE 0.000 0.000
27.32
138 A-FARNESENE OR 0.311 0.110
ZINGIBERENE
27.53
139 CAS# 030021-74-0 0.087 0.000
27.75
143 A-MUUROLENE 0.107 0.170
27.90
144 B-SELINENE 0.508 0.000
28.04
145 A-SELINENE 0.533 0.050
28.34
147 METHYL 0.195 0.000
?-UNDECENOATE
29.16
154 D-CADINENE 0.624 0.420
29.39
155 G-CADINENE 0.306 0.246
30.34
164 CAS# 016728-99-7 OR
0.071 0.000
G-SELINENE
30.73
166 A-MUUROLINE 0.066 0.000
31.15
170 2-TRIDECANONE 0.836 0.565
31.36
171 GERANYL PROPANOATE
0.402 0.218
31.46
172 MW 212 METHYL 0.109 0.069
?-DODECENOATE
32.28
177 trans-GERANIOL 0.092 0.762
32.54
179 CAS# 000483-77-2 0.000 0.000
(CALAMENENE ?)
34.38
187 METHYL 3,6- 0.047 0.098
DODECDIENOATE ME 79
36.42
196 MW 210 ME 43,54,82
0.148 0.000
39.11
211 CARYOPHYLLENE OXIDE
0.075 0.072
40.23
221 HUMULENE 0.000 0.000
MONO-EPOXIDE I
40.28
223 MW222 ME 43,82 0.064 0.000
41.07
225 HUMULENE 0.157 0.114
MONO-EPOXIDE II
41.55
228 HUMULENE 0.000 0.000
MONO-EPOXIDE III
41.88
231 MW 222 ME 43,79,80
0.490 0.164
43.88
242 MW 220 ME 43,79,91
0.382 0.000
44.73
246 T-CADINOL 0.069 0.000
46.08
255 T-MUUROLOL 0.000 0.000
ME 95,121,204
47.50
262 MW 220 ME 41,67,109
0.000 0.000
DIEPOXIDE?
48.69
268 FARNESOL 0.093 0.000
______________________________________
GC/ TENTATIVE IDENTI-
21043
FID CMPD. FICATION BY GC./ CHALL- 21226
RT. # MASS SPECTROSCOPY
ANGER CHINOCK
______________________________________
6.42 9 A-PINENE 0.078 0.102
7.46 14 ISOBUTYL ISOBUTYRATE
0.192 0.270
8.10 16 8-PINENE 0.765 0.768
9.11 18 MYRCENE 55.224 48.105
9.77 22 2-METHYL-BUTYL 0.254 0.477
PROPANOATE
9.90 23 ISOAMYL ISOBUTYRATE
0.082 0.447
9.96 24 2-METHYL-BUTYL 1.164 2.385
ISOBUTARATE
10.28
26 L-LIMONENE 0.204 0.210
10.62
27 B-PHELLANDRENE? 0.260 0.228
C10H15
11.59
32 trans-OCIMENE 0.259 0.067
12.45
35 2-METHYL-BUTYL 0.067 0.185
2-METHYL-BUTYRATE
12.68
36 METHYL HEPTANOATE
0.631 0.200
12.91
40 ISOAMYL ISOVALERATE
0.000 0.398
14.31
43 METHYL 4-METHYL- 0.337 0.099
HEXA-2-ENOATE
14.39
45 METHYL 6-METHYL- 0.241 1.211
HEPTANOATE
15.98
51 METHYL OCTANOATE 0.323 0.199
16.26
55 NONANAL 0.076 0.043
19.41
73 METHYL NONANOATE 0.000 0.162
19.56
75 2-DECANONE, 0.189 0.158
A-YLANGENE
19.93
77 A-COPAENE 0.164 0.530
21.00
83 LINALOOL 0.714 0.400
21.31
88 A-GURJUNENE 0.000 0.696
21.42
89 7-METHYL-2-DECANONE
0.076 0.079
22.56
93 C15H24 CALARENE? 0.109 0.186
22.72
95 A-BERGAMOTENE, 0.094 0.156
METHYL DECANOATE
23.01
96 2-UNDECANONE 0.793 0.127
23.10
97 2-DECANOL 0.227 0.453
23.44
99 8-CARYOPHYLLENE 5.394 6.439
23.84
102 METHYL DEC-4-ENOATE
0.760 1.180
24.03
103 MW168 2-OL? 0.105 0.044
ME 43,81,110,108
25.18
114 B-FARNESENE 1.041 0.000
26.10
122 HUMULENE 17.304 14.547
26.16
123 METHYL DEC-4,8- 0.000 0.583
DIENOATE
26.46
129 WILEY# 123998 MW 204
0.232 1.511
C15H24
26.59
131 2-DODECANONE 0.403 0.000
27.32
138 A-FARNESENE OR 0.319 0.362
ZINGIBERENE
27.53
139 CAS# 030021-74-0 0.099 0.331
27.75
143 A-MUUROLENE 0.148 0.510
27.90
144 B-SELINENE 2.769 0.886
28.04
145 A-SELINENE 3.204 0.971
28.34
147 METHYL 0.241 0.000
?-UNDECENOATE
29.16
154 D-CADINENE 0.810 2.685
29.39
155 G-CADINENE 0.409 1.493
30.34
164 CAS# 016728-99-7 OR
0.105 1.965
G-SELINENE
30.73
166 A-MUUROLINE 0.000 0.288
31.15
170 2-TRIDECANONE 0.397 0.512
31.36
171 GERANYL PROPANOATE
0.000 0.088
31.46
172 MW 212 METHYL 0.000 0.074
?-DODECENOATE
32.28
177 trans-GERANIOL 0.000 0.979
32.54
179 CAS# 000483-77-2 0.061 0.346
(CALAMENENE ?)
34.38
187 METHYL 3,6- 0.000 0.446
DODECDIENOATE ME 79
36.42
196 MW 210 ME 43,54,82
0.135 0.089
39.11
211 CARYOPHYLLENE OXIDE
0.106 0.066
40.23
221 HUMULENE 0.048 0.131
MONO-EPOXIDE I
40.28
223 MW222 ME 43,82 0.232 0.122
41.07
225 HUMULENE 0.250 0.153
MONO-EPOXIDE II
41.55
228 HUMULENE 0.063 0.136
MONO-EPOXIDE III
41.88
231 MW 222 ME 43,79,80
0.356 0.268
43.88
242 MW 220 ME 43,79,91
0.163 0.000
44.73
246 T-CADINOL 0.077 0.248
46.08
255 T-MUUROLOL 0.057 0.119
ME 95,121,204
47.50
262 MW 220 ME 41,67,109
0.000 0.000
DIEPOXIDE?
48.69
268 FARNESOL 0.076 0.524
______________________________________
GC/ TENTATIVE IDENTI-
65103 65103
FID CMPD. FICATION BY GC./ CLUS- CLUS-
RT. # MASS SPECTROSCOPY
TER L-1 TER L-2
______________________________________
6.42 9 A-PINENE 0.103 0.153
7.46 14 ISOBUTYL ISOBUTYRATE
0.280 0.153
8.10 16 8-PINENE 0.866 0.655
9.11 18 MYRCENE 60.712 45.653
9.77 22 2-METHYL-BUTYL 0.536 0.335
PROPANOATE
9.90 23 ISOAMYL ISOBUTYRATE
0.483 0.525
9.96 24 2-METHYL-BUTYL 1.641 1.178
ISOBUTARATE
10.28
26 L-LIMONENE 0.268 0.235
10.62
27 B-PHELLANDRENE? 0.295 0.210
C10H15
11.59
32 trans-OCIMENE 0.370 0.229
12.45
35 2-METHYL-BUTYL 0.075 0.070
2-METHYL-BUTYRATE
12.68
36 METHYL HEPTANOATE
0.244 0.220
12.91
40 ISOAMYL ISOVALERATE
0.187 0.271
14.31
43 METHYL 4-METHYL- 0.342 0.310
HEXA-2-ENOATE
14.39
45 METHYL 6-METHYL- 0.447 0.399
HEPTANOATE
15.98
51 METHYL OCTANOATE 0.721 0.555
16.26
55 NONANAL 0.250 0.467
19.41
73 METHYL NONANOATE 0.165 0.158
19.56
75 2-DECANONE, 0.060 0.100
A-YLANGENE
19.93
77 A-COPAENE 0.126 0.242
21.00
83 LINALOOL 0.347 0.259
21.31
88 A-GURJUNENE 0.372 0.374
21.42
89 7-METHYL-2-DECANONE
0.071 0.075
22.56
93 C15H24 CALARENE? 0.082 0.158
22.72
95 A-BERGAMOTENE, 0.953 0.855
METHYL DECANOATE
23.01
96 2-UNDECANONE 0.533 0.648
23.10
97 2-DECANOL 0.180 0.352
23.44
99 8-CARYOPHYLLENE 4.725 8.080
23.84
102 METHYL DEC-4-ENOATE
1.801 1.927
24.03
103 MW168 2-OL? 0.073 0.108
ME 43,81,110,108
25.18
114 B-FARNESENE 0.000 0.000
26.10
122 HUMULENE 13.195 20.805
26.16
123 METHYL DEC-4,8- 0.154 0.132
DIENOATE
26.46
129 WILEY# 123998 MW 204
0.306 0.538
C15H24
26.59
131 2-DODECANONE 0.064 0.078
27.32
138 A-FARNESENE OR 0.135 0.197
ZINGIBERENE
27.53
139 CAS# 030021-74-0 0.084 0.137
27.75
143 A-MUUROLENE 0.127 0.216
27.90
144 B-SELINENE 0.319 0.558
28.04
145 A-SELINENE 0.356 0.612
28.34
147 METHYL 0.000 0.000
?-UNDECENOATE
29.16
154 D-CADINENE 0.655 1.137
29.39
155 G-CADINENE 0.330 0.604
30.34
164 CAS# 016728-99-7 OR
0.081 0.141
G-SELINENE
30.73
166 A-MUUROLINE 0.059 0.110
31.15
170 2-TRIDECANONE 0.843 1.060
31.36
171 GERANYL PROPANOATE
0.000 0.000
31.46
172 MW 212 METHYL 0.741 0.925
?-DODECENOATE
32.28
177 trans-GERANIOL 0.703 0.446
32.54
179 CAS# 000483-77-2 0.000 0.108
(CALAMENENE ?)
34.38
187 METHYL 3,6- 0.228 0.221
DODECDIENOATE ME 79
36.42
196 MW 210 ME 43,54,82
0.062 0.082
39.11
211 CARYOPHYLLENE OXIDE
0.094 0.251
40.23
221 HUMULENE 0.000 0.147
MONO-EPOXIDE I
40.28
223 MW222 ME 43,82 0.151 0.201
41.07
225 HUMULENE 0.237 0.653
MONO-EPOXIDE II
41.55
228 HUMULENE 0.000 0.137
MONO-EPOXIDE III
41.88
231 MW 222 ME 43,79,80
0.666 1.036
43.88
242 MW 220 ME 43,79,91
0.429 0.609
44.73
246 T-CADINOL 0.088 0.170
46.08
255 T-MUUROLOL 0.000 0.090
ME 95,121,204
47.50
262 MW 220 ME 41,67,109
0.000 0.077
DIEPOXIDE?
48.69
268 FARNESOL 0.066 0.084
______________________________________
GC/ TENTATIVE IDENTI-
FID CMPD. FICATION BY GC./ 21220 21016
RT. # MASS SPECTROSCOPY
EROICA FUGGLE
______________________________________
6.42 9 A-PINENE 0.155 0.075
7.46 14 ISOBUTYL ISOBUTYRATE
0.000 0.221
8.10 16 8-PINENE 0.884 0.712
9.11 18 MYRCENE 61.773 54.039
9.77 22 2-METHYL-BUTYL 0.237 0.157
PROPANOATE
9.90 23 ISOAMYL ISOBUTYRATE
0.124 0.000
9.96 24 2-METHYL-BUTYL 0.549 0.443
ISOBUTARATE
10.28
26 L-LIMONENE 0.233 0.186
10.62
27 B-PHELLANDRENE? 0.264 0.242
C10H15
11.59
32 trans-OCIMENE 1.577 0.105
12.45
35 2-METHYL-BUTYL 0.000 0.000
2-METHYL-BUTYRATE
12.68
36 METHYL HEPTANOATE
0.292 0.426
12.91
40 ISOAMYL ISOVALERATE
0.000 0.000
14.31
43 METHYL 4-METHYL- 0.000 0.170
HEXA-2-ENOATE
14.39
45 METHYL 6-METHYL- 0.271 0.114
HEPTANOATE
15.98
51 METHYL OCTANOATE 0.257 0.088
16.26
55 NONANAL 0.070 0.098
19.41
73 METHYL NONANOATE 0.204 0.097
19.56
75 2-DECANONE, 0.161 0.212
A-YLANGENE
19.93
77 A-COPAENE 0.053 0.225
21.00
83 LINALOOL 0.508 0.651
21.31
88 A-GURJUNENE 0.155 0.000
21.42
89 7-METHYL-2-DECANONE
0.214 0.083
22.56
93 C15H24 CALARENE? 0.000 0.157
22.72
95 A-BERGAMOTENE, 0.325 0.272
METHYL DECANOATE
23.01
96 2-UNDECANONE 0.820 0.406
23.10
97 2-DECANOL 0.000 0.348
23.44
99 8-CARYOPHYLLENE 12.756 7.628
23.84
102 METHYL DEC-4-ENOATE
0.875 0.409
24.03
103 MW168 2-OL? 0.167 0.122
ME 43,81,110,108
25.18
114 B-FARNESENE 0.000 4.870
26.10
122 HUMULENE 0.355 20.059
26.16
123 METHYL DEC-4,8- 0.419 0.147
DIENOATE
26.46
129 WILEY# 123998 MW 204
0.104 0.528
C15H24
26.59
131 2-DODECANONE 0.344 0.073
27.32
138 A-FARNESENE OR 0.000 0.543
ZINGIBERENE
27.53
139 CAS# 030021-74-0 0.000 0.090
27.75
143 A-MUUROLENE 0.060 0.173
27.90
144 B-SELINENE 2.268 0.177
28.04
145 A-SELINENE 2.562 0.186
28.34
147 METHYL 0.000 0.424
?-UNDECENOATE
29.16
154 D-CADINENE 0.293 1.092
29.39
155 G-CADINENE 0.167 0.573
30.34
164 CAS# 016728-99-7 OR
0.000 0.147
G-SELINENE
30.73
166 A-MUUROLINE 0.000 0.117
31.15
170 2-TRIDECANONE 0.606 0.397
31.36
171 GERANYL PROPANOATE
0.091 0.000
31.46
172 MW 212 METHYL 0.000 0.000
?-DODECENOATE
32.28
177 trans-GERANIOL 0.949 0.000
32.54
179 CAS# 000483-77-2 0.077 0.080
(CALAMENENE ?)
34.38
187 METHYL 3,6- 0.228 0.064
DODECDIENOATE ME 79
36.42
196 MW 210 ME 43,54,82
0.437 0.080
39.11
211 CARYOPHYLLENE OXIDE
0.156 0.126
40.23
221 HUMULENE 0.164 0.000
MONO-EPOXIDE I
40.28
223 MW222 ME 43,82 0.878 0.087
41.07
225 HUMULENE 0.000 0.279
MONO-EPOXIDE II
41.55
228 HUMULENE 0.000 0.088
MONO-EPOXIDE III
41.88
231 MW 222 ME 43,79,80
1.761 0.435
43.88
242 MW 220 ME 43,79,91
0.461 0.235
44.73
246 T-CADINOL 0.055 0.125
46.08
255 T-MUUROLOL 0.000 0.070
ME 95,121,204
47.50
262 MW 220 ME 41,67,109
0.000 0.000
DIEPOXIDE?
48.69
268 FARNESOL 0.326 0.134
______________________________________
GC/ TENTATIVE IDENTI- 21014
FID CMPD. FICATION BY GC./ 21102 HALL-
RT. # MASS SPECTROSCOPY
GALENA ERTAU
______________________________________
6.42 9 A-PINENE 0.109 0.064
7.46 14 ISOBUTYL ISOBUTYRATE
0.479 0.151
8.10 16 8-PINENE 0.027 0.653
9.11 18 MYRCENE 55.179 53.508
9.77 22 2-METHYL-BUTYL 1.009 0.184
PROPANOATE
9.90 23 ISOAMYL ISOBUTYRATE
0.554 0.000
9.96 24 2-METHYL-BUTYL 5.004 0.278
ISOBUTARATE
10.28
26 L-LIMONENE 0.218 0.158
10.62
27 B-PHELLANDRENE? 0.239 0.204
C10H15
11.59
32 trans-OCIMENE 2.260 0.144
12.45
35 2-METHYL-BUTYL 0.151 0.000
2-METHYL-BUTYRATE
12.68
36 METHYL HEPTANOATE
0.427 0.647
12.91
40 ISOAMYL ISOVALERATE
0.169 0.000
14.31
43 METHYL 4-METHYL- 0.152 0.264
HEXA-2-ENOATE
14.39
45 METHYL 6-METHYL- 0.930 0.150
HEPTANOATE
15.98
51 METHYL OCTANOATE 0.878 0.203
16.26
55 NONANAL 0.079 0.095
19.41
73 METHYL NONANOATE 0.436 0.138
19.56
75 2-DECANONE, 0.071 0.258
A-YLANGENE
19.93
77 A-COPAENE 0.111 0.214
21.00
83 LINALOOL 0.206 0.939
21.31
88 A-GURJUNENE 0.577 0.044
21.42
89 7-METHYL-2-DECANONE
0.009 0.086
22.56
93 C15H24 CALARENE? 0.053 0.120
22.72
95 A-BERGAMOTENE, 1.317 0.000
METHYL DECANOATE
23.01
96 2-UNDECANONE 0.386 0.696
23.10
97 2-DECANOL 0.131 0.337
23.44
99 8-CARYOPHYLLENE 4.602 7.238
23.84
102 METHYL DEC-4-ENOATE
2.237 0.660
24.03
103 MW168 2-OL? 0.042 0.157
ME 43,81,110,108
25.18
114 B-FARNESENE 0.000 0.000
26.10
122 HUMULENE 10.599 24.799
26.16
123 METHYL DEC-4,8- 0.180 0.000
DIENOATE
26.46
129 WILEY# 123998 MW 204
0.281 0.670
C15H24
26.59
131 2-DODECANONE 0.076 0.132
27.32
138 A-FARNESENE OR 0.000 0.227
ZINGIBERENE
27.53
139 CAS# 030021-74-0 0.054 0.113
27.75
143 A-MUUROLENE 0.101 0.214
27.90
144 B-SELINENE 0.465 0.190
28.04
145 A-SELINENE 0.544 0.207
28.34
147 METHYL 0.000 0.069
?-UNDECENOATE
29.16
154 D-CADINENE 0.586 1.250
29.39
155 G-CADINENE 0.300 0.653
30.34
164 CAS# 016728-99-7 OR
0.142 0.151
G-SELINENE
30.73
166 A-MUUROLINE 0.064 0.136
31.15
170 2-TRIDECANONE 1.561 0.421
31.36
171 GERANYL PROPANOATE
0.119 0.000
31.46
172 MW 212 METHYL 0.219 0.000
?-DODECENOATE
32.28
177 trans-GERANIOL 0.000 0.000
32.54
179 CAS# 000483-77-2 0.000 0.102
(CALAMENENE ?)
34.38
187 METHYL 3,6- 1.725 0.071
DODECDIENOATE ME 79
36.42
196 MW 210 ME 43,54,82
0.186 0.125
39.11
211 CARYOPHYLLENE OXIDE
0.064 0.111
40.23
221 HUMULENE 0.000 0.099
MONO-EPOXIDE I
40.28
223 MW222 ME 43,82 0.237 0.239
41.07
225 HUMULENE 0.134 0.357
MONO-EPOXIDE II
41.55
228 HUMULENE 0.000 0.100
MONO-EPOXIDE III
41.88
231 MW 222 ME 43,79,80
0.579 0.638
43.88
242 MW 220 ME 43,79,91
0.263 0.263
44.73
246 T-CADINOL 0.059 0.125
46.08
255 T-MUUROLOL 0.000 0.075
ME 95,121,204
47.50
262 MW 220 ME 41,67,109
0.000 0.000
DIEPOXIDE?
48.69
268 FARNESOL 0.000 0.064
______________________________________
GC/ TENTATIVE IDENTI-
21135 21097
FID CMPD. FICATION BY GC./ HERS- HULLERER
RT. # MASS SPECTROSCOPY
BRUCK BITTERER
______________________________________
6.42 9 A-PINENE 0.000 0.087
7.46 14 ISOBUTYL ISOBUTYRATE
0.000 0.088
8.10 16 8-PINENE 0.527 0.737
9.11 18 MYRCENE 44.828 52.818
9.77 22 2-METHYL-BUTYL 0.000 0.415
PROPANOATE
9.90 23 ISOAMYL ISOBUTYRATE
0.000 0.168
9.96 24 2-METHYL-BUTYL 0.066 0.844
ISOBUTARATE
10.28
26 L-LIMONENE 0.147 0.207
10.62
27 B-PHELLANDRENE? 0.145 0.212
C10H15
11.59
32 trans-OCIMENE 0.000 0.092
12.45
35 2-METHYL-BUTYL 0.000 0.079
2-METHYL-BUTYRATE
12.68
36 METHYL HEPTANOATE
0.418 0.231
12.91
40 ISOAMYL ISOVALERATE
0.000 0.075
14.31
43 METHYL 4-METHYL- 0.289 0.000
HEXA-2-ENOATE
14.39
45 METHYL 6-METHYL- 0.171 0.408
HEPTANOATE
15.98
51 METHYL OCTANOATE 0.134 0.110
16.26
55 NONANAL 0.226 0.084
19.41
73 METHYL NONANOATE 0.090 0.088
19.56
75 2-DECANONE, 0.334 0.251
A-YLANGENE
19.93
77 A-COPAENE 0.259 0.617
21.00
83 LINALOOL 0.805 0.503
21.31
88 A-GURJUNENE 0.077 0.169
21.42
89 7-METHYL-2-DECANONE
0.214 0.121
22.56
93 C15H24 CALARENE? 0.190 0.300
22.72
95 A-BERGAMOTENE, 0.000 0.000
METHYL DECANOATE
23.01
96 2-UNDECANONE 0.921 0.387
23.10
97 2-DECANOL 0.531 0.602
23.44
99 8-CARYOPHYLLENE 5.573 4.386
23.84
102 METHYL DEC-4-ENOATE
0.551 0.772
24.03
103 MW168 2-OL? 0.210 0.122
ME 43,81,110,108
25.18
114 B-FARNESENE 0.324 0.093
26.10
122 HUMULENE 29.352 8.090
26.16
123 METHYL DEC-4,8- 0.132 0.352
DIENOATE
26.46
129 WILEY# 123998 MW 204
0.735 1.994
C15H24
26.59
131 2-DODECANONE 0.203 0.000
27.32
138 A-FARNESENE OR 0.326 0.273
ZINGIBERENE
27.53
139 CAS# 030021-74-0 0.103 0.352
27.75
143 A-MUUROLENE 0.248 0.574
27.90
144 B-SELINENE 0.306 2.221
28.04
145 A-SELINENE 0.335 2.426
28.34
147 METHYL 0.191 0.399
?-UNDECENOATE
29.16
154 D-CADINENE 1.330 2.714
29.39
155 G-CADINENE 0.746 1.516
30.34
164 CAS# 016728-99-7 OR
0.141 3.993
G-SELINENE
30.73
166 A-MUUROLINE 0.146 0.364
31.15
170 2-TRIDECANONE 0.580 0.241
31.36
171 GERANYL PROPANOATE
0.000 0.000
31.46
172 MW 212 METHYL 0.000 0.000
?-DODECENOATE
32.28
177 trans-GERANIOL 0.000 0.134
32.54
179 CAS# 000483-77-2 0.229 0.205
(CALAMENENE ?)
34.38
187 METHYL 3,6- 0.000 0.340
DODECDIENOATE ME 79
36.42
196 MW 210 ME 43,54,82
0.147 0.179
39.11
211 CARYOPHYLLENE OXIDE
0.331 0.082
40.23
221 HUMULENE 0.145 0.137
MONO-EPOXIDE I
40.28
223 MW222 ME 43,82 0.260 0.000
41.07
225 HUMULENE 0.962 0.194
MONO-EPOXIDE II
41.55
228 HUMULENE 0.159 0.074
MONO-EPOXIDE III
41.88
231 MW 222 ME 43,79,80
0.752 0.531
43.88
242 MW 220 ME 43,79,91
0.489 0.195
44.73
246 T-CADINOL 0.177 0.336
46.08
255 T-MUUROLOL 0.114 0.132
ME 95,121,204
47.50
262 MW 220 ME 41,67,109
0.000 0.000
DIEPOXIDE?
48.69
268 FARNESOL 0.161 0.166
______________________________________
GC/ TENTATIVE IDENTI- 21113
FID CMPD. FICATION BY GC./ 21457 LUB-
RT. # MASS SPECTROSCOPY
LIBERTY ELSKA
______________________________________
6.42 9 A-PINENE 0.043 0.047
7.46 14 ISOBUTYL ISOBUTYRATE
0.068 0.000
8.10 16 8-PINENE 0.504 0.421
9.11 18 MYRCENE 41.506 29.558
9.77 22 2-METHYL-BUTYL 0.117 0.000
PROPANOATE
9.90 23 ISOAMYL ISOBUTYRATE
0.000 0.000
9.96 24 2-METHYL-BUTYL 0.197 0.176
ISOBUTARATE
10.28
26 L-LIMONENE 0.124 0.145
10.62
27 B-PHELLANDRENE? 0.149 0.099
C10H15
11.59
32 trans-OCIMENE 0.066 0.000
12.45
35 2-METHYL-BUTYL 0.000 0.000
2-METHYL-BUTYRATE
12.68
36 METHYL HEPTANOATE
0.293 0.259
12.91
40 ISOAMYL ISOVALERATE
0.000 0.000
14.31
43 METHYL 4-METHYL- 0.128 0.081
HEXA-2-ENOATE
14.39
45 METHYL 6-METHYL- 0.083 0.148
HEPTANOATE
15.98
51 METHYL OCTANOATE 0.189 0.100
16.26
55 NONANAL 0.091 0.193
19.41
73 METHYL NONANOATE 0.092 0.183
19.56
75 2-DECANONE, 0.240 0.268
A-YLANGENE
19.93
77 A-COPAENE 0.304 0.268
21.00
83 LINALOOL 0.814 0.254
21.31
88 A-GURJUNENE 0.043 0.106
21.42
89 7-METHYL-2-DECANONE
0.098 0.198
22.56
93 C15H24 CALARENE? 0.172 0.179
22.72
95 A-BERGAMOTENE, 0.000 0.947
METHYL DECANOATE
23.01
96 2-UNDECANONE 0.737 0.690
23.10
97 2-DECANOL 0.442 0.443
23.44
99 8-CARYOPHYLLENE 9.651 6.953
23.84
102 METHYL DEC-4-ENOATE
0.663 1.089
24.03
103 MW168 2-OL? 0.140 0.207
ME 43,81,110,108
25.18
114 B-FARNESENE 0.080 17.712
26.10
122 HUMULENE 32.402 21.404
26.16
123 METHYL DEC-4,8- 0.075 0.451
DIENOATE
26.46
129 WILEY# 123998 MW 204
0.387 0.796
C15H24
26.59
131 2-DODECANONE 0.105 0.167
27.32
138 A-FARNESENE OR 0.509 0.316
ZINGIBERENE
27.53
139 CAS# 030021-74-0 0.181 0.157
27.75
143 A-MUUROLENE 0.289 0.282
27.90
144 B-SELINENE 0.325 1.492
28.04
145 A-SELINENE 0.358 1.546
28.34
147 METHYL 0.310 0.242
?-UNDECENOATE
29.16
154 D-CADINENE 1.508 1.438
29.39
155 G-CADINENE 0.887 0.811
30.34
164 CAS# 016728-99-7 OR
0.213 0.237
G-SELINENE
30.73
166 A-MUUROLINE 0.103 0.159
31.15
170 2-TRIDECANONE 0.458 0.738
31.36
171 GERANYL PROPANOATE
0.000 0.000
31.46
172 MW 212 METHYL 0.000 0.070
?-DODECENOATE
32.28
177 trans-GERANIOL 0.099 0.257
32.54
179 CAS# 000483-77-2 0.193 0.196
(CALAMENENE ?)
34.38
187 METHYL 3,6- 0.057 0.203
DODECDIENOATE ME 79
36.42
196 MW 210 ME 43,54,82
0.115 0.199
39.11
211 CARYOPHYLLENE OXIDE
0.205 0.550
40.23
221 HUMULENE 0.103 0.298
MONO-EPOXIDE I
40.28
223 MW222 ME 43,82 0.308 0.261
41.07
225 HUMULENE 0.599 1.612
MONO-EPOXIDE II
41.55
228 HUMULENE 0.144 0.000
MONO-EPOXIDE III
41.88
231 MW 222 ME 43,79,80
0.568 1.358
43.88
242 MW 220 ME 43,79,91
0.342 0.439
44.73
246 T-CADINOL 0.179 0.247
46.08
255 T-MUUROLOL 0.128 0.131
ME 95,121,204
47.50
262 MW 220 ME 41,67,109
0.000 0.094
DIEPOXIDE?
48.69
268 FARNESOL 0.340 0.339
______________________________________
21073
GC/ TENTATIVE IDENTI-
21114 NORTH-
FID CMPD. FICATION BY GC./ NADWIS- ERN
RT. # MASS SPECTROSCOPY
LANSKA BREWER
______________________________________
6.42 9 A-PINENE 0.066 0.125
7.46 14 ISOBUTYL ISOBUTYRATE
0.000 0.174
8.10 16 8-PINENE 0.649 0.862
9.11 18 MYRCENE 44.179 55.587
9.77 22 2-METHYL-BUTYL 0.000 0.183
PROPANOATE
9.90 23 ISOAMYL ISOBUTYRATE
0.000 0.108
9.96 24 2-METHYL-BUTYL 0.000 0.428
ISOBUTARATE
10.28
26 L-LIMONENE 0.169 0.248
10.62
27 B-PHELLANDRENE? 0.149 0.265
C10H15
11.59
32 trans-OCIMENE 0.000 0.488
12.45
35 2-METHYL-BUTYL 0.000 0.000
2-METHYL-BUTYRATE
12.68
36 METHYL HEPTANOATE
0.391 0.706
12.91
40 ISOAMYL ISOVALERATE
0.000 0.000
14.31
43 METHYL 4-METHYL- 0.206 0.251
HEXA-2-ENOATE
14.39
45 METHYL 6-METHYL- 0.178 0.150
HEPTANOATE
15.98
51 METHYL OCTANOATE 0.113 0.199
16.26
55 NONANAL 0.141 0.077
19.41
73 METHYL NONANOATE 0.155 0.166
19.56
75 2-DECANONE, 0.260 0.177
A-YLANGENE
19.93
77 A-COPAENE 0.160 0.284
21.00
83 LINALOOL 0.390 0.344
21.31
88 A-GURJUNENE 0.073 0.000
21.42
89 7-METHYL-2-DECANONE
0.116 0.049
22.56
93 C15H24 CALARENE? 0.113 0.154
22.72
95 A-BERGAMOTENE, 0.854 0.000
METHYL DECANOATE
23.01
96 2-UNDECANONE 0.592 0.292
23.10
97 2-DECANOL 0.284 0.307
23.44
99 8-CARYOPHYLLENE 5.415 9.217
23.84
102 METHYL DEC-4-ENOATE
0.939 0.565
24.03
103 MW168 2-OL? 0.211 0.077
ME 43,81,110,108
25.18
114 B-FARNESENE 17.417 0.000
26.10
122 HUMULENE 18.740 21.642
26.16
123 METHYL DEC-4,8- 0.368 0.389
DIENOATE
26.46
129 WILEY# 123998 MW 204
0.461 0.619
C15H24
26.59
131 2-DODECANONE 0.127 0.084
27.32
138 A-FARNESENE OR 0.264 0.179
ZINGIBERENE
27.53
139 CAS# 030021-74-0 0.053 0.112
27.75
143 A-MUUROLENE 0.143 0.217
27.90
144 B-SELINENE 0.217 0.182
28.04
145 A-SELINENE 0.161 0.210
28.34
147 METHYL 0.206 0.000
?-UNDECENOATE
29.16
154 D-CADINENE 0.766 1.301
29.39
155 G-CADINENE 0.431 0.650
30.34
164 CAS# 016728-99-7 OR
0.121 0.129
G-SELINENE
30.73
166 A-MUUROLINE 0.085 0.124
31.15
170 2-TRIDECANONE 0.417 0.337
31.36
171 GERANYL PROPANOATE
0.000 0.000
31.46
172 MW 212 METHYL 0.000 0.000
?-DODECENOATE
32.28
177 trans-GERANIOL 0.200 0.000
32.54
179 CAS# 000483-77-2 0.131 0.110
(CALAMENENE ?)
34.38
187 METHYL 3,6- 0.146 0.068
DODECDIENOATE ME 79
36.42
196 MW 210 ME 43,54,82
0.127 0.082
39.11
211 CARYOPHYLLENE OXIDE
0.284 0.122
40.23
221 HUMULENE 0.156 0.065
MONO-EPOXIDE I
40.28
223 MW222 ME 43,82 0.127 0.109
41.07
225 HUMULENE 0.855 0.250
MONO-EPOXIDE II
41.55
228 HUMULENE 0.112 0.090
MONO-EPOXIDE III
41.88
231 MW 222 ME 43,79,80
0.755 0.295
43.88
242 MW 220 ME 43,79,91
0.210 0.169
44.73
246 T-CADINOL 0.113 0.119
46.08
255 T-MUUROLOL 0.045 0.057
ME 95,121,204
47.50
262 MW 220 ME 41,67,109
0.000 0.000
DIEPOXIDE?
48.69
268 FARNESOL 0.187 0.230
______________________________________
GC/ TENTATIVE IDENTI-
21044
FID CMPD. FICATION BY GC./ NORTH- 21197
RT. # MASS SPECTROSCOPY
DOWN NUGGET
______________________________________
6.42 9 A-PINENE 0.066 0.095
7.46 14 ISOBUTYL ISOBUTYRATE
0.000 0.244
8.10 16 8-PINENE 0.639 0.671
9.11 18 MYRCENE 42.280 60.676
9.77 22 2-METHYL-BUTYL 0.106 0.559
PROPANOATE
9.90 23 ISOAMYL ISOBUTYRATE
0.000 0.550
9.96 24 2-METHYL-BUTYL 0.173 1.307
ISOBUTARATE
10.28
26 L-LIMONENE 0.173 0.203
10.62
27 B-PHELLANDRENE? 0.199 0.244
C10H15
11.59
32 trans-OCIMENE 0.104 0.571
12.45
35 2-METHYL-BUTYL 0.000 0.063
2-METHYL-BUTYRATE
12.68
36 METHYL HEPTANOATE
0.337 0.615
12.91
40 ISOAMYL ISOVALERATE
0.000 0.124
14.31
43 METHYL 4-METHYL- 0.127 0.201
HEXA-2-ENOATE
14.39
45 METHYL 6-METHYL- 0.098 0.257
HEPTANOATE
15.98
51 METHYL OCTANOATE 0.165 0.470
16.26
55 NONANAL 0.000 0.052
19.41
73 METHYL NONANOATE 0.122 0.326
19.56
75 2-DECANONE, 0.000 0.052
A-YLANGENE
19.93
77 A-COPAENE 0.321 0.167
21.00
83 LINALOOL 0.559 0.904
21.31
88 A-GURJUNENE 0.000 0.113
21.42
89 7-METHYL-2-DECANONE
0.000 0.000
22.56
93 C15H24 CALARENE? 0.228 0.069
22.72
95 A-BERGAMOTENE, 0.000 0.298
METHYL DECANOATE
23.01
96 2-UNDECANONE 0.181 0.363
23.10
97 2-DECANOL 0.474 0.230
23.44
99 8-CARYOPHYLLENE 12.260 6.289
23.84
102 METHYL DEC-4-ENOATE
0.798 1.366
24.03
103 MW168 2-OL? 0.000 0.054
ME 43,81,110,108
25.18
114 B-FARNESENE 0.746 0.000
26.10
122 HUMULENE 31.313 15.386
26.16
123 METHYL DEC-4,8- 0.000 0.128
DIENOATE
26.46
129 WILEY# 123998 MW 204
0.198 0.359
C15H24
26.59
131 2-DODECANONE 0.800 0.077
27.32
138 A-FARNESENE OR 0.210 0.321
ZINGIBERENE
27.53
139 CAS# 030021-74-0 0.153 0.086
27.75
143 A-MUUROLENE 0.300 0.237
27.90
144 B-SELINENE 0.291 0.560
28.04
145 A-SELINENE 0.324 0.647
28.34
147 METHYL 0.000 0.164
?-UNDECENOATE
29.16
154 D-CADINENE 1.517 0.760
29.39
155 G-CADINENE 0.815 0.399
30.34
164 CAS# 016728-99-7 OR
0.146 0.102
G-SELINENE
30.73
166 A-MUUROLINE 0.163 0.075
31.15
170 2-TRIDECANONE 0.149 0.500
31.36
171 GERANYL PROPANOATE
0.000 0.000
31.46
172 MW 212 METHYL 0.000 0.077
?-DODECENOATE
32.28
177 trans-GERANIOL 0.000 0.000
32.54
179 CAS# 000483-77-2 0.199 0.000
(CALAMENENE ?)
34.38
187 METHYL 3,6- 0.000 0.119
DODECDIENOATE ME 79
36.42
196 MW 210 ME 43,54,82
0.000 0.112
39.11
211 CARYOPHYLLENE OXIDE
0.410 0.051
40.23
221 HUMULENE 0.089 0.000
MONO-EPOXIDE I
40.28
223 MW222 ME 43,82 0.067 0.328
41.07
225 HUMULENE 0.801 0.113
MONO-EPOXIDE II
41.55
228 HUMULENE 0.153 0.000
MONO-EPOXIDE III
41.88
231 MW 222 ME 43,79,80
0.168 0.643
43.88
242 MW 220 ME 43,79,91
0.101 0.206
44.73
246 T-CADINOL 0.138 0.062
46.08
255 T-MUUROLOL 0.086 0.000
ME 95,121,204
47.50
262 MW 220 ME 41,67,109
0.000 0.000
DIEPOXIDE?
48.69
268 FARNESOL 0.154 0.071
______________________________________
GC/ TENTATIVE IDENTI-
FID CMPD. FICATION BY GC./ 21223 21227
RT. # MASS SPECTROSCOPY
OLYMPIC PERLE
______________________________________
6.42 9 A-PINENE 0.127 0.077
7.46 14 ISOBUTYL ISOBUTYRATE
0.116 0.193
8.10 16 8-PINENE 1.037 0.618
9.11 18 MYRCENE 61.466 41.489
9.77 22 2-METHYL-BUTYL 0.470 0.120
PROPANOATE
9.90 23 ISOAMYL ISOBUTYRATE
0.121 0.000
9.96 24 2-METHYL-BUTYL 1.424 0.308
ISOBUTARATE
10.28
26 L-LIMONENE 0.247 0.177
10.62
27 B-PHELLANDRENE? 0.269 0.199
C10H15
11.59
32 trans-OCIMENE 0.040 0.591
12.45
35 2-METHYL-BUTYL 0.549 0.000
2-METHYL-BUTYRATE
12.68
36 METHYL HEPTANOATE
0.136 0.425
12.91
40 ISOAMYL ISOVALERATE
0.476 0.000
14.31
43 METHYL 4-METHYL- 0.051 0.111
HEXA-2-ENOATE
14.39
45 METHYL 6-METHYL- 0.216 0.061
HEPTANOATE
15.98
51 METHYL OCTANOATE 0.066 0.053
16.26
55 NONANAL 0.053 0.096
19.41
73 METHYL NONANOATE 0.040 0.047
19.56
75 2-DECANONE, 0.056 0.256
A-YLANGENE
19.93
77 A-COPAENE 0.201 0.350
21.00
83 LINALOOL 0.316 0.242
21.31
88 A-GURJUNENE 0.096 0.000
21.42
89 7-METHYL-2-DECANONE
0.000 0.047
22.56
93 C15H24 CALARENE? 0.093 0.224
22.72
95 A-BERGAMOTENE, 0.055 0.000
METHYL DECANOATE
23.01
96 2-UNDECANONE 0.000 0.319
23.10
97 2-DECANOL 0.238 0.487
23.44
99 8-CARYOPHYLLENE 8.722 12.480
23.84
102 METHYL DEC-4-ENOATE
0.743 0.150
24.03
103 MW168 2-OL? 0.000 0.096
ME 43,81,110,108
25.18
114 B-FARNESENE 0.000 0.162
26.10
122 HUMULENE 13.614 31.397
26.16
123 METHYL DEC-4,8- 0.408 0.175
DIENOATE
26.46
129 WILEY# 123998 MW 204
0.518 0.845
C15H24
26.59
131 2-DODECANONE 0.000 0.110
27.32
138 A-FARNESENE OR 0.177 0.296
ZINGIBERENE
27.53
139 CAS# 030021-74-0 0.116 0.183
27.75
143 A-MUUROLENE 0.248 0.315
27.90
144 B-SELINENE 0.738 0.276
28.04
145 A-SELINENE 0.764 0.320
28.34
147 METHYL 0.000 0.000
?-UNDECENOATE
29.16
154 D-CADINENE 0.979 1.770
29.39
155 G-CADINENE 0.547 0.862
30.34
164 CAS# 016728-99-7 OR
0.116 0.190
G-SELINENE
30.73
166 A-MUUROLINE 0.107 0.177
31.15
170 2-TRIDECANONE 0.300 0.273
31.36
171 GERANYL PROPANOATE
0.105 0.000
31.46
172 MW 212 METHYL 0.065 0.000
?-DODECENOATE
32.28
177 trans-GERANIOL 1.055 0.000
32.54
179 CAS# 000483-77-2 0.084 0.123
(CALAMENENE ?)
34.38
187 METHYL 3,6- 0.063 0.000
DODECDIENOATE ME 79
36.42
196 MW 210 ME 43,54,82
0.000 0.077
39.11
211 CARYOPHYLLENE OXIDE
0.166 0.152
40.23
221 HUMULENE 0.059 0.072
MONO-EPOXIDE I
40.28
223 MW222 ME 43,82 0.113 0.100
41.07
225 HUMULENE 0.224 0.366
MONO-EPOXIDE II
41.55
228 HUMULENE 0.076 0.136
MONO-EPOXIDE III
41.88
231 MW 222 ME 43,79,80
0.253 0.389
43.88
242 MW 220 ME 43,79,91
0.139 0.276
44.73
246 T-CADINOL 0.097 0.186
46.08
255 T-MUUROLOL 0.057 0.115
ME 95,121,204
47.50
262 MW 220 ME 41,67,109
0.000 0.000
DIEPOXIDE?
48.69
268 FARNESOL 0.127 0.192
______________________________________
GC/ TENTATIVE IDENTI-
60052
FID CMPD. FICATION BY GC./ PRIDE OF 21077
RT. # MASS SPECTROSCOPY
RINGWOOD SAAZ
______________________________________
6.42 9 A-PINENE 0.089 0.049
7.46 14 ISOBUTYL ISOBUTYRATE
0.043 0.000
8.10 16 8-PINENE 0.690 0.486
9.11 18 MYRCENE 46.727 34.050
9.77 22 2-METHYL-BUTYL 0.121 0.000
PROPANOATE
9.90 23 ISOAMYL ISOBUTYRATE
0.000 0.000
9.96 24 2-METHYL-BUTYL 0.220 0.000
ISOBUTARATE
10.28
26 L-LIMONENE 0.197 0.166
10.62
27 B-PHELLANDRENE? 0.156 0.081
C10H15
11.59
32 trans-OCIMENE 0.000 0.000
12.45
35 2-METHYL-BUTYL 0.000 0.000
2-METHYL-BUTYRATE
12.68
36 METHYL HEPTANOATE
0.334 0.626
12.91
40 ISOAMYL ISOVALERATE
0.000 0.000
14.31
43 METHYL 4-METHYL- 0.180 0.258
HEXA-2-ENOATE
14.39
45 METHYL 6-METHYL- 0.319 0.340
HEPTANOATE
15.98
51 METHYL OCTANOATE 0.473 0.225
16.26
55 NONANAL 0.116 0.308
19.41
73 METHYL NONANOATE 0.250 0.380
19.56
75 2-DECANONE, 0.147 0.501
A-YLANGENE
19.93
77 A-COPAENE 0.241 0.191
21.00
83 LINALOOL 0.317 0.337
21.31
88 A-GURJUNENE 0.230 0.173
21.42
89 7-METHYL-2-DECANONE
0.125 0.222
22.56
93 C15H24 CALARENE? 0.133 0.113
22.72
95 A-BERGAMOTENE, 0.326 1.043
METHYL DECANOATE
23.01
96 2-UNDECANONE 0.690 1.394
23.10
97 2-DECANOL 0.226 0.299
23.44
99 8-CARYOPHYLLENE 8.708 5.010
23.84
102 METHYL DEC-4-ENOATE
1.808 1.769
24.03
103 MW168 2-OL? 0.230 0.417
ME 43,81,110,108
25.18
114 B-FARNESENE 0.000 18.900
26.10
122 HUMULENE 1.946 18.111
26.16
123 METHYL DEC-4,8- 0.571 0.259
DIENOATE
26.46
129 WILEY# 123998 MW 204
0.680 0.531
C15H24
26.59
131 2-DODECANONE 0.053 0.264
27.32
138 A-FARNESENE OR 1.932 0.255
ZINGIBERENE
27.53
139 CAS# 030021-74-0 0.000 0.134
27.75
143 A-MUUROLENE 0.000 0.189
27.90
144 B-SELINENE 9.372 0.255
28.04
145 A-SELINENE 10.128 0.178
28.34
147 METHYL 1.329 0.104
?-UNDECENOATE
29.16
154 D-CADINENE 1.124 0.829
29.39
155 G-CADINENE 0.650 0.519
30.34
164 CAS# 016728-99-7 OR
0.137 0.181
G-SELINENE
30.73
166 A-MUUROLINE 0.133 0.099
31.15
170 2-TRIDECANONE 0.551 0.957
31.36
171 GERANYL PROPANOATE
0.000 0.000
31.46
172 MW 212 METHYL 0.104 0.081
?-DODECENOATE
32.28
177 trans-GERANIOL 0.217 0.080
32.54
179 CAS# 000483-77-2 0.146 0.167
(CALAMENENE ?)
34.38
187 METHYL 3,6- 0.182 0.424
DODECDIENOATE ME 79
36.42
196 MW 210 ME 43,54,82
0.079 0.272
39.11
211 CARYOPHYLLENE OXIDE
0.337 0.476
40.23
221 HUMULENE 0.076 0.329
MONO-EPOXIDE I
40.28
223 MW222 ME 43,82 0.175 0.294
41.07
225 HUMULENE 0.106 1.530
MONO-EPOXIDE II
41.55
228 HUMULENE 0.082 0.155
MONO-EPOXIDE III
41.88
231 MW 222 ME 43,79,80
0.852 1.789
43.88
242 MW 220 ME 43,79,91
0.231 0.487
44.73
246 T-CADINOL 0.140 0.174
46.08
255 T-MUUROLOL 0.067 0.099
ME 95,121,204
47.50
262 MW 220 ME 41,67,109
0.000 0.083
DIEPOXIDE?
48.69
268 FARNESOL 0.169 0.160
______________________________________
GC/ TENTATIVE IDENTI- 21173
FID CMPD. FICATION BY GC./ 21186 STRISS-
RT. # MASS SPECTROSCOPY
SPALTER LESPALT
______________________________________
6.42 9 A-PINENE 0.091 0.062
7.46 14 ISOBUTYL ISOBUTYRATE
0.000 0.202
8.10 16 8-PINENE 0.861 0.634
9.11 18 MYRCENE 60.356 43.977
9.77 22 2-METHYL-BUTYL 0.000 0.257
PROPANOATE
9.90 23 ISOAMYL ISOBUTYRATE
0.000 0.112
9.96 24 2-METHYL-BUTYL 0.000 0.736
ISOBUTARATE
10.28
26 L-LIMONENE 0.225 0.175
10.62
27 B-PHELLANDRENE? 0.234 0.139
C10H15
11.59
32 trans-OCIMENE 0.055 0.082
12.45
35 2-METHYL-BUTYL 0.000 0.000
2-METHYL-BUTYRATE
12.68
36 METHYL HEPTANOATE
0.658 0.326
12.91
40 ISOAMYL ISOVALERATE
0.000 0.000
14.31
43 METHYL 4-METHYL- 0.238 0.323
HEXA-2-ENOATE
14.39
45 METHYL 6-METHYL- 0.230 0.000
HEPTANOATE
15.98
51 METHYL OCTANOATE 0.168 0.103
16.26
55 NONANAL 0.166 0.255
19.41
73 METHYL NONANOATE 0.341 0.119
19.56
75 2-DECANONE, 0.313 0.216
A-YLANGENE
19.93
77 A-COPAENE 0.090 0.293
21.00
83 LINALOOL 0.557 0.649
21.31
88 A-GURJUNENE 0.127 0.000
21.42
89 7-METHYL-2-DECANONE
0.144 0.133
22.56
93 C15H24 CALARENE? 0.055 0.243
22.72
95 A-BERGAMOTENE, 0.691 0.000
METHYL DECANOATE
23.01
96 2-UNDECANONE 0.612 0.385
23.10
97 2-DECANOL 0.179 0.509
23.44
99 8-CARYOPHYLLENE 2.481 10.500
23.84
102 METHYL DEC-4-ENOATE
1.180 0.570
24.03
103 MW168 2-OL? 0.211 0.091
ME 43,81,110,108
25.18
114 B-FARNESENE 14.019 0.000
26.10
122 HUMULENE 9.083 29.252
26.16
123 METHYL DEC-4,8- 0.313 0.147
DIENOATE
26.46
129 WILEY# 123998 MW 204
0.243 0.872
C15H24
26.59
131 2-DODECANONE 0.174 0.000
27.32
138 A-FARNESENE OR 0.179 0.131
ZINGIBERENE
27.53
139 CAS# 030021-74-0 0.000 0.088
27.75
143 A-MUUROLENE 0.137 0.233
27.90
144 B-SELINENE 0.130 0.237
28.04
145 A-SELINENE 0.069 0.281
28.34
147 METHYL 0.118 0.082
?-UNDECENOATE
29.16
154 D-CADINENE 0.398 1.364
29.39
155 G-CADINENE 0.226 0.824
30.34
164 CAS# 016728-99-7 OR
0.119 0.210
G-SELINENE
30.73
166 A-MUUROLINE 0.000 0.196
31.15
170 2-TRIDECANONE 0.479 0.255
31.36
171 GERANYL PROPANOATE
0.000 0.000
31.46
172 MW 212 METHYL 0.063 0.000
?-DODECENOATE
32.28
177 trans-GERANIOL 0.193 0.000
32.54
179 CAS# 000483-77-2 0.073 0.205
(CALAMENENE ?)
34.38
187 METHYL 3,6- 0.233 0.000
DODECDIENOATE ME 79
36.42
196 MW 210 ME 43,54,82
0.155 0.172
39.11
211 CARYOPHYLLENE OXIDE
0.139 0.540
40.23
221 HUMULENE 0.106 0.152
MONO-EPOXIDE I
40.28
223 MW222 ME 43,82 0.137 0.150
41.07
225 HUMULENE 0.425 1.140
MONO-EPOXIDE II
41.55
228 HUMULENE 0.000 0.000
MONO-EPOXIDE III
41.88
231 MW 222 ME 43,79,80
0.854 0.609
43.88
242 MW 220 ME 43,79,91
0.186 0.141
44.73
246 T-CADINOL 0.064 0.144
46.08
255 T-MUUROLOL 0.000 0.092
ME 95,121,204
47.50
262 MW 220 ME 41,67,109
0.000 0.000
DIEPOXIDE?
48.69
268 FARNESOL 0.093 0.191
______________________________________
GC/ TENTATIVE IDENTI-
FID CMPD. FICATION BY GC./ 21049 21112
RT. # MASS SPECTROSCOPY
STYRIAN TARGET
______________________________________
6.42 9 A-PINENE 0.089 0.119
7.46 14 ISOBUTYL ISOBUTYRATE
0.328 0.406
8.10 16 8-PINENE 0.795 0.865
9.11 18 MYRCENE 56.296 58.164
9.77 22 2-METHYL-BUTYL 0.171 0.482
PROPANOATE
9.90 23 ISOAMYL ISOBUTYRATE
0.000 0.461
9.96 24 2-METHYL-BUTYL 0.753 1.408
ISOBUTARATE
10.28
26 L-LIMONENE 0.207 0.228
10.62
27 B-PHELLANDRENE? 0.229 0.226
C10H15
11.59
32 trans-OCIMENE 0.057 0.366
12.45
35 2-METHYL-BUTYL 0.000 0.054
2-METHYL-BUTYRATE
12.68
36 METHYL HEPTANOATE
0.270 0.602
12.91
40 ISOAMYL ISOVALERATE
0.000 0.118
14.31
43 METHYL 4-METHYL- 0.305 0.427
HEXA-2-ENOATE
14.39
45 METHYL 6-METHYL- 0.000 0.589
HEPTANOATE
15.98
51 METHYL OCTANOATE 0.000 0.446
16.26
55 NONANAL 0.141 0.107
19.41
73 METHYL NONANOATE 0.070 0.689
19.56
75 2-DECANONE, 0.216 0.821
A-YLANGENE
19.93
77 A-COPAENE 0.198 0.336
21.00
83 LINALOOL 0.751 0.891
21.31
88 A-GURJUNENE 0.000 0.000
21.42
89 7-METHYL-2-DECANONE
0.094 0.401
22.56
93 C15H24 CALARENE? 0.166 0.000
22.72
95 A-BERGAMOTENE, 0.266 0.519
METHYL DECANOATE
23.01
96 2-UNDECANONE 0.301 1.502
23.10
97 2-DECANOL 0.366 0.282
23.44
99 8-CARYOPHYLLENE 7.269 4.952
23.84
102 METHYL DEC-4-ENOATE
0.196 1.912
24.03
103 MW168 2-OL? 0.096 0.214
ME 43,81,110,108
25.18
114 B-FARNESENE 4.187 0.000
26.10
122 HUMULENE 20.607 10.291
26.16
123 METHYL DEC-4,8- 0.106 0.221
DIENOATE
26.46
129 WILEY# 123998 MW 204
0.527 1.123
C15H24
26.59
131 2-DODECANONE 0.000 0.000
27.32
138 A-FARNESENE OR 0.000 0.233
ZINGIBERENE
27.53
139 CAS# 030021-74-0 0.000 0.165
27.75
143 A-MUUROLENE 0.211 0.215
27.90
144 B-SELINENE 0.152 0.329
28.04
145 A-SELINENE 0.184 0.404
28.34
147 METHYL 0.269 0.000
?-UNDECENOATE
29.16
154 D-CADINENE 0.013 1.215
29.39
155 G-CADINENE 0.536 0.653
30.34
164 CAS# 016728-99-7 OR
0.000 0.798
G-SELINENE
30.73
166 A-MUUROLINE 0.167 0.198
31.15
170 2-TRIDECANONE 0.228 0.974
31.36
171 GERANYL PROPANOATE
0.000 0.000
31.46
172 MW 212 METHYL 0.000 0.114
?-DODECENOATE
32.28
177 trans-GERANIOL 0.000 0.000
32.54
179 CAS# 000483-77-2 0.112 0.118
(CALAMENENE ?)
34.38
187 METHYL 3,6- 0.000 0.388
DODECDIENOATE ME 79
36.42
196 MW 210 ME 43,54,82
0.000 0.460
39.11
211 CARYOPHYLLENE OXIDE
0.265 0.095
40.23
221 HUMULENE 0.000 0.536
MONO-EPOXIDE I
40.28
223 MW222 ME 43,82 0.000 0.000
41.07
225 HUMULENE 0.569 0.210
MONO-EPOXIDE II
41.55
228 HUMULENE 0.000 0.000
MONO-EPOXIDE III
41.88
231 MW 222 ME 43,79,80
0.394 1.253
43.88
242 MW 220 ME 43,79,91
0.119 0.266
44.73
246 T-CADINOL 0.119 0.114
46.08
255 T-MUUROLOL 0.000 0.000
ME 95,121,204
47.50
262 MW 220 ME 41,67,109
0.000 0.000
DIEPOXIDE?
48.69
268 FARNESOL 0.000 0.000
______________________________________
GC/ TENTATIVE IDENTI-
21015 21041
FID CMPD. FICATION BY GC./ TETT- WTILAM-
RT. # MASS SPECTROSCOPY
NANG EITF
______________________________________
6.42 9 A-PINENE 0.080 0.078
7.46 14 ISOBUTYL ISOBUTYRATE
0.000 0.217
8.10 16 8-PINENE 0.753 0.741
9.11 18 MYRCENE 50.137 55.492
9.77 22 2-METHYL-BUTYL 0.000 0.234
PROPANOATE
9.90 23 ISOAMYL ISOBUTYRATE
0.000 0.000
9.96 24 2-METHYL-BUTYL 0.000 0.507
ISOBUTARATE
10.28
26 L-LIMONENE 0.205 0.191
10.62
27 B-PHELLANDRENE? 0.147 0.251
C10H15
11.59
32 trans-OCIMENE 0.000 0.114
12.45
35 2-METHYL-BUTYL 0.000 0.000
2-METHYL-BUTYRATE
12.68
36 METHYL HEPTANOATE
0.509 0.532
12.91
40 ISOAMYL ISOVALERATE
0.000 0.000
14.31
43 METHYL 4-METHYL- 0.230 0.224
HEXA-2-ENOATE
14.39
45 METHYL 6-METHYL- 0.260 0.120
HEPTANOATE
15.98
51 METHYL OCTANOATE 0.172 0.087
16.26
55 NONANAL 0.233 0.073
19.41
73 METHYL NONANOATE 0.228 0.078
19.56
75 2-DECANONE, 0.290 0.097
A-YLANGENE
19.93
77 A-COPAENE 0.129 0.190
21.00
83 LINALOOL 0.441 0.661
21.31
88 A-GURJUNENE 0.125 0.000
21.42
89 7-METHYL-2-DECANONE
0.199 0.000
22.56
93 C15H24 CALARENE? 0.085 0.141
22.72
95 A-BERGAMOTENE, 0.873 0.380
METHYL DECANOATE
23.01
96 2-UNDECANONE 0.759 0.131
23.10
97 2-DECANOL 0.257 0.313
23.44
99 8-CARYOPHYLLENE 3.697 6.934
23.84
102 METHYL DEC-4-ENOATE
1.220 0.288
24.03
103 MW168 2-OL? 0.255 0.041
ME 43,81,110,108
25.18
114 B-FARNESENE 16.938 7.097
26.10
122 HUMULENE 13.277 18.815
26.16
123 METHYL DEC-4,8- 0.338 0.196
DIENOATE
26.46
129 WILEY# 123998 MW 204
0.321 0.472
C15H24
26.59
131 2-DODECANONE 0.169 0.000
27.32
138 A-FARNESENE OR 0.194 0.599
ZINGIBERENE
27.53
139 CAS# 030021-74-0 0.000 0.106
27.75
143 A-MUUROLENE 0.121 0.170
27.90
144 B-SELINENE 0.166 0.245
28.04
145 A-SELINENE 0.099 0.238
28.34
147 METHYL 0.177 0.407
?-UNDECENOATE
29.16
154 D-CADINENE 0.532 0.970
29.39
155 G-CADINENE 0.317 0.493
30.34
164 CAS# 016728-99-7 OR
0.114 0.107
G-SELINENE
30.73
166 A-MUUROLINE 0.059 0.099
31.15
170 2-TRIDECANONE 0.574 0.142
31.36
171 GERANYL PROPANOATE
0.000 0.000
31.46
172 MW 212 METHYL 0.000 0.000
?-DODECENOATE
32.28
177 trans-GERANIOL 0.157 0.050
32.54
179 CAS# 000483-77-2 0.131 0.071
(CALAMENENE ?)
34.38
187 METHYL 3,6- 0.192 0.000
DODECDIENOATE ME 79
36.42
196 MW 210 ME 43,54,82
0.163 0.000
39.11
211 CARYOPHYLLENE OXIDE
0.282 0.097
40.23
221 HUMULENE 0.180 0.000
MONO-EPOXIDE I
40.28
223 MW222 ME 43,82 0.161 0.000
41.07
225 HUMULENE 0.901 0.200
MONO-EPOXIDE II
41.55
228 HUMULENE 0.089 0.071
MONO-EPOXIDE III
41.88
231 MW 222 ME 43,79,80
1.033 0.118
43.88
242 MW 220 ME 43,79,91
0.235 0.000
44.73
246 T-CADINOL 0.098 0.104
46.08
255 T-MUUROLOL 0.000 0.061
ME 95,121,204
47.50
262 MW 220 ME 41,67,109
0.000 0.000
DIEPOXIDE?
48.69
268 FARNESOL 0.131 0.457
______________________________________
GC/ TENTATIVE IDENTI- 9/17
FID CMPD. FICATION BY GC./ 21498 COLUM-
RT. # MASS SPECTROSCOPY
YEOMAN BUS
______________________________________
6.42 9 A-PINENE 0.121 0.125
7.46 14 ISOBUTYL ISOBUTYRATE
0.455 0.066
8.10 16 8-PINENE 0.829 0.745
9.11 18 MYRCENE 51.427 42.267
9.77 22 2-METHYL-BUTYL 0.659 0.370
PROPANOATE
9.90 23 ISOAMYL ISOBUTYRATE
0.522 0.184
9.96 24 2-METHYL-BUTYL 1.946 0.800
ISOBUTARATE
10.28
26 L-LIMONENE 0.237 0.236
10.62
27 B-PHELLANDRENE? 0.260 0.177
C10H15
11.59
32 trans-OCIMENE 0.159 0.168
12.45
35 2-METHYL-BUTYL 0.180 0.090
2-METHYL-BUTYRATE
12.68
36 METHYL HEPTANOATE
0.349 0.000
12.91
40 ISOAMYL ISOVALERATE
0.274 0.106
14.31
43 METHYL 4-METHYL- 0.139 0.000
HEXA-2-ENOATE
14.39
45 METHYL 6-METHYL- 0.119 0.153
HEPTANOATE
15.98
51 METHYL OCTANOATE 0.209 0.000
16.26
55 NONANAL 0.059 0.000
19.41
73 METHYL NONANOATE 0.070 0.000
19.56
75 2-DECANONE, 0.133
A-YLANGENE
19.93
77 A-COPAENE 0.252 0.764
21.00
83 LINALOOL 0.379 0.417
21.31
88 A-GURJUNENE 0.000
21.42
89 7-METHYL-2-DECANONE
0.000 0.000
22.56
93 C15H24 CALARENE? 0.123 0.252
22.72
95 A-BERGAMOTENE, 0.000 0.000
METHYL DECANOATE
23.01
96 2-UNDECANONE 0.361 0.068
23.10
97 2-DECANOL 0.268
23.44
99 8-CARYOPHYLLENE 6.954 10.236
23.84
102 METHYL DEC-4-ENOATE
0.692 0.457
24.03
103 MW168 2-OL? 0.115 0.000
ME 43,81,110,108
25.18
114 B-FARNESENE 0.000 0.000
26.10
122 HUMULENE 10.146 17.370
26.16
123 METHYL DEC-4,8- 0.553
DIENOATE
26.46
129 WILEY# 123998 MW 204
0.439 1.992
C15H24
26.59
131 2-DODECANONE 0.045 0.000
27.32
138 A-FARNESENE OR 0.223 0.508
ZINGIBERENE
27.53
139 CAS# 030021-74-0 0.117 0.464
27.75
143 A-MUUROLENE 0.209 0.733
27.90
144 B-SELINENE 2.829 1.230
28.04
145 A-SELINENE 3.352 1.291
28.34
147 METHYL 0.075 0.000
?-UNDECENOATE
29.16
154 D-CADINENE 1.028 3.412
29.39
155 G-CADINENE 0.484 2.048
30.34
164 CAS# 016728-99-7 OR
0.134 2.809
G-SELINENE
30.73
166 A-MUUROLINE 0.095 0.366
31.15
170 2-TRIDECANONE 0.313 0.000
31.36
171 GERANYL PROPANOATE
0.000 0.753
31.46
172 MW 212 METHYL 0.000 0.000
?-DODECENOATE
32.28
177 trans-GERANIOL 0.363 0.180
32.54
179 CAS# 000483-77-2 0.069 0.381
(CALAMENENE ?)
34.38
187 METHYL 3,6- 0.061 0.000
DODECDIENOATE ME 79
36.42
196 MW 210 ME 43,54,82
0.048 0.135
39.11
211 CARYOPHYLLENE OXIDE
0.061 0.235
40.23
221 HUMULENE 0.059 0.109
MONO-EPOXIDE I
40.28
223 MW222 ME 43,82 0.078
41.07
225 HUMULENE 0.161 0.358
MONO-EPOXIDE II
41.55
228 HUMULENE 0.073 0.167
MONO-EPOXIDE III
41.88
231 MW 222 ME 43,79,80
0.365 0.847
43.88
242 MW 220 ME 43,79,91
0.000 0.455
44.73
246 T-CADINOL 0.101 0.232
46.08
255 T-MUUROLOL 0.062 0.111
ME 95,121,204
47.50
262 MW 220 ME 41,67,109
0.000 0.112
DIEPOXIDE?
48.69
268 FARNESOL 0.148
______________________________________
APPENDIX 2
______________________________________
Detailed GLC analyses of Columbus and other U.S. high alpha
______________________________________
hops.
Area %
COLUMBUS GALENA
Identification
Group 92-12-8-2 92-12-8-3
______________________________________
Apha-Pinene 0.125 0.184
Beta-Pinene 1 0.943 0.883
Myrcene 1 51.571 66.560
Methyl butyl Isobutyrate
0.838 3.575
Limonene 4 0.288 0.350
Linalool 3 0.470 0.294
Caryophyllene 1 6.297 3.097
Farnesene? 1 0.275 0.112
Humulene 1 11.049 5.764
Muurolene 4 0.768 0.309
Selinene 4 1.472 0.341
Geranyl Acetate
3 0.016 0.048
delta Cadinene
4 1.834 0.292
Gamma Cadinene
4 1.095 0.143
Geranyl Isobutyrate
3 1.716 0.296
Undecanone-2 1.348 0.059
Geraniol 3 0.048 0.060
Limonen-10-ol 0.155 0.021
Caryophyllene oxide
2 0.259 0.014
Humulene epoxide I
2 0.430 0.033
Humulene epoxide II
2 1.450 0.068
Caryolan-i-ol 2 0.120 0.006
Humulene epoxide III
2 0.025 0.013
Humulol 2 0.185 0.011
beta Eudesmol?
alpha Eudesmol?
Humulenol II 2 0.103
Humulene diepoxide?
2
Major Hydrocarbons (1) 70.135 76.216
Oxidation Products (2) 2.572 0.145
Floral-Estery (3) 2.250 0.698
Citrus Piney (4) 5.457 1.435
Total Accounted For 80.414 78.494
Humulene/Caryophyllene 1.753 1.861
______________________________________
CHINOOK NUGGET
Identification
Group 92-12-9-1 92-12-9-2
______________________________________
Apha-Pinene 0.047 0.081
Beta-Pinene 1 0.435 0.667
Myrcene 1 28.469 51.972
Methyl butyl Isobutyrate
1.410 1.274
Limonene 4 0.136 0.183
Linalool 3 0.369 1.022
Caryophyllene 1 9.516 8.597
Farnesene? 1 0.353 0.162
Humulene 1 21.157 19.029
Muurolene 4 1.517 0.921
Selinene 4 1.385 0.818
Geranyl Acetate
3 0.210 0.044
delta Cadinene
4 4.215 0.926
Gamma Cadinene
4 2.686 0.445
Geranyl Isobutyrate
3 3.323 0.076
Undecanone-2 0.675 0.453
Geraniol 3 0.155 0.079
Limonen-10-ol 0.049 0.037
Caryophyllene oxide
2 0.076 0.032
Humulene epoxide I
2 0.326 0.220
Humulene epoxide II
2 0.663 0.328
Caryolan-i-ol 2 0.088 0.015
Humulene epoxide III
2 0.033
Humulol 2 0.379 0.040
beta Eudesmol? 0.248
alpha Eudesmol? 0.205
Humulenol II 2 0.112 0.022
Humulene diepoxide?
2 0.316
Major Hydrocarbons (1) 59.924 80.427
Oxidation Products (2) 1.993 0.657
Floral-Estery (3) 4.057 1.221
Citrus Piney (4) 9.939 3.293
Total Accounted For 75.913 85.598
Humulene/Caryophyllene 2.223 2.213
______________________________________
Area Percent Report
Sample Name: 92-12-8-2
Pk # Ret Time Area Area %
Identification
______________________________________
1 7.030 3884 0.031
2 7.854 1456 0.011
3 8.010 1580 0.012
4 9.053 8364 0.066
5 9.260 10821 0.086
6 9.358 15718 0.125 Alpha-Pinene
7 9.800 4213 0.033
8 10.297 2957 0.023
9 10.543 9679 0.077
10 11.097 967 0.007
11 11.242 118386 0.943 Beta-Pinene
12 11.904 1183 0.009
13 12.476 6468547 51.571
Myrcene
14 12.685 4762 0.038
15 12.746 3884 0.031
16 13.064 52106 0.415
17 13.204 48568 0.387
18 13.274 105192 0.838 Methyl butyl Isobutyrate
19 13.586 36238 0.288 Limonene
20 13.797 3396 0.027
21 13.938 26704 0.212
22 14.149 4588 0.036
23 14.333 8748 0.069
24 14.520 1416 0.011
25 14.602 16543 0.131
26 14.879 17328 0.138
27 15.087 7345 0.058
28 15.212 931 0.007
29 15.420 743 0.005
30 15.506 3693 0.029
31 15.722 3398 0.027
32 15.801 10636 0.084
33 16.009 5267 0.042
34 16.069 2526 0.020
35 16.244 25684 0.204
36 16.624 3088 0.024
37 16.793 10279 0.081
38 16.915 3626 0.028
39 17.427 1969 0.015
40 17.673 67587 0.538
41 18.248 2557 0.020
42 18.354 1000 0.008
43 18.423 691 0.005
44 18.953 7042 0.056
45 19.178 7410 0.059
46 19.291 6033 0.048
47 19.466 10779 0.085
48 19.777 7142 0.056
49 19.956 4956 0.039
50 20.134 23743 0.189
51 20.364 804 0.006
52 20.742 2092 0.016
53 20.877 5423 0.043
54 20.984 1676 0.013
55 21.043 923 0.007
56 21.229 29717 0.236
57 21.448 1539 0.012
58 21.532 7494 0.059
59 21.843 8179 0.065
60 22.211 1838 0.014
61 22.295 6856 0.054
62 22.441 19924 0.158
63 22.568 2933 0.023
64 22.750 66342 0.528
65 22.922 5479 0.043
66 23.076 2236 0.017
67 23.224 1919 0.015
68 23.360 6240 0.049
69 23.682 59058 0.470 Linalool
70 23.938 25935 0.206
71 24.171 19672 0.156
72 24.285 1535 0.012
73 24.432 6320 0.050
74 24.542 1797 0.014
75 24.816 10688 0.085
76 25.028 9189 0.073
77 25.276 29524 0.235
78 25.457 3016 0.024
79 25.597 16721 0.133
80 25.737 4624 0.036
81 25.858 45802 0.365
82 26.177 789857 6.297 Caryophyllene
83 26.267 147160 1.173
84 26.483 11876 0.094
85 26.654 2115 0.016
86 26.951 19239 0.153
87 27.128 7807 0.062
88 27.262 34560 0.275 Farnesene?
89 27.452 5485 0.043
90 27.650 12130 0.096
91 27.741 32092 0.255
92 28.029 22059 0.175
93 28.286 1385879 11.049
Humulene
94 28.518 174316 1.389
95 28.816 1217 0.009
96 29.099 46594 0.371
97 29.289 65255 0.520
98 29.452 50235 0.400
99 29.663 101538 0.809
100 29.743 96334 0.768 Muurolene
101 29.922 184741 1.472 Selinene
102 30.066 2003 0.016 Geranyl Acetate
103 30.168 16997 0.135
104 30.380 230089 1.834 delta Cadinene
105 30.604 137367 1.095 Gamma Cadiene
106 30.702 18692 0.149
107 30.952 9850 0.078
108 31.085 4796 0.038
109 31.312 215300 1.716 Geranyl Isobutyrate
110 31.411 169139 1.348 Undecanone-2
111 31.517 26715 0.213
112 31.630 77466 0.617
113 31.737 5913 0.047
114 31.821 6115 0.048 Geraniol
115 32.106 9621 0.076
116 32.224 20686 0.164
117 32.501 13220 0.105
118 32.620 30790 0.245
119 33.031 7712 0.061
120 33.254 2445 0.019
121 33.462 7790 0.062
122 33.722 28110 0.224
123 33.960 4270 0.034
124 34.081 2094 0.016
125 34.192 4256 0.033
126 34.312 4289 0.034
127 34.372 5764 0.046
128 34.483 5031 0.040
129 34.565 5092 0.040
131 34.901 34301 0.273
132 35.009 13488 0.107
133 35.126 5975 0.047
134 35.299 1614 0.012
135 35.487 11667 0.093
136 35.854 17035 0.135 Limonen-10-ol
137 36.161 2233 0.017
138 36.312 32546 0.259 Caryophyllene oxide
139 36.476 1067 0.008
140 36.577 2462 0.019
141 36.935 6949 0.055
142 37.071 1073 0.008
143 37.206 13125 0.104
144 37.370 2081 0.016
145 37.555 7101 0.056
146 37.672 54047 0.430 Humulene epoxide I
147 38.006 8150 0.065
148 38.127 3162 0.025
149 38.444 2423 0.019
150 38.688 30971 0.246
151 38.915 181869 1.450 Humulene epoxide II
152 39.075 15063 0.120 Caryolan-1-ol
153 39.222 3225 0.025 Humulene epoxide III
154 39.501 7444 0.059
155 39.809 1283 0.010
156 39.935 1171 0.009
157 40.158 5912 0.047
158 40.396 1243 0.009
159 40.677 52277 0.416
160 40.871 8343 0.066
161 41.254 6706 0.053
162 41.404 23253 0.185 Humulol
163 41.759 4806 0.038
164 41.946 30832 0.245
165 42.077 2776 0.022
166 42.236 2521 0.020
167 42.537 2687 0.021
168 42.964 11140 0.088
169 43.065 7509 0.059
170 43.258 23800 0.189
171 43.848 4438 0.035
172 44.637 12985 0.103 Humulenol II
173 45.039 5691 0.045
174 45.892 3866 0.030
175 46.597 1815 0.014
176 47.014 2417 0.019
177 50.412 1109 0.008
= 1.25428E + 007
______________________________________
Area Percent Report
Sample Name: 92-12-8-3
Pk # Ret Time Area Area %
Identification
______________________________________
1 7.034 2371 0.022
2 7.855 753 0.007
3 7.988 864 0.008
4 8.623 706 0.066
5 9.056 1684 0.016
6 9.262 2429 0.023
7 9.359 19367 0.184 alpha Pinene
8 9.801 6546 0.062
9 10.096 924 0.008
10 10.318 10977 0.104
11 10.547 49458 0.469
12 10.920 660 0.006
13 11.098 1561 0.014
14 11.242 93039 0.883 beta Pinene
15 11.733 1061 0.010
16 11.901 3162 0.030
17 12.090 1322 0.012
18 12.484 6985773 66.360
Myrcene
19 12.692 7230 0.068
20 12.750 5559 0.052
21 13.073 155982 1.481
22 13.217 83592 0.794
23 13.301 376416 3.575 Methylbutyl Isobutyrate
24 13.593 36853 0.350 Limoene
25 13.810 1808 0.017
26 13.942 30257 0.287
27 14.151 2083 0.019
28 14.317 15305 0.145
29 14.529 2092 0.019
30 14.609 73712 0.700
31 14.896 239176 2.272
32 15.091 5970 0.056
33 15.333 699 0.006
34 15.508 2556 0.024
35 15.713 3795 0.036
36 15.804 17078 0.162
37 16.011 59153 0.561
38 16.246 26755 0.254
39 16.818 12944 0.123
40 17.427 1728 0.016
41 17.682 151069 1.435
42 18.253 1689 0.016
43 18.730 932 0.008
44 18.953 1326 0.012
45 19.192 122003 1.159
46 19.329 5927 0.056
47 19.481 11385 0.108
48 19.791 7946 0.075
49 19.952 1333 0.012
50 20.134 5804 0.055
51 20.727 9706 0.092
52 20.879 21447 0.203
53 21.054 2750 0.026
54 21.227 17557 0.166
55 21.769 2599 0.024
56 22.298 42843 0.407
57 22.437 4686 0.044
58 22.494 4842 0.046
59 22.739 15483 0.147
60 22.234 720 0.006
61 23.358 6212 0.059
62 23.681 30996 0.294 Linalool
63 23.937 41114 0.390
64 24.168 9021 0.085
65 24.789 2293 0.021
66 25.025 2599 0.024
67 25.343 82950 0.788
68 25.449 1561 0.014
69 25.596 28078 0.266
70 25.830 3277 0.031
71 26.073 326058 3.097 Caryophyllene
72 26.267 160273 1.522
73 26.482 8286 0.078
74 26.695 887 0.008
75 26.833 1478 0.014
76 26.923 20836 0.197
77 27.019 1307 0.012
78 27.112 1737 0.016
79 27.223 11802 0.112 Farnesene?
80 27.440 2763 0.026
81 27.709 6688 0.063
82 28.023 24366 0.231
83 28.227 606830 5.764 Humulene
84 28.353 4325 0.041
85 28.485 24982 0.237
86 28.582 4950 0.047
87 28.782 837 0.008
88 29.082 8038 0.076
89 29.251 9309 0.088
90 29.429 7845 0.074
91 29.642 32547 0.309 Muurolene
92 29.716 35968 0.341 Selinene
93 29.883 5145 0.048 Geranyl Acetate
94 30.139 3103 0.029
95 30.328 30746 0.292 delta Cadinene
96 30.562 15061 0.143 gamma Cadinene
97 30.780 1170 0.011
98 31.068 2479 0.023
99 31.144 7554 0.071
100 31.383 31189 0.296 Geranyl Isobutyrate
101 31.504 6271 0.059 Undecanone-2
102 31.610 3590 0.034
103 31.720 6384 0.060 Geraniol
104 32.097 2303 0.021
105 32.222 1438 0.013
106 32.489 10866 0.103
107 32.608 3493 0.033
108 33.028 780 0.007
109 33.719 42378 0.402
110 33.949 697 0.006
111 34.181 786 0.007
112 34.478 1455 0.013
113 34.754 699 0.006
114 34.891 1927 0.018
115 35.004 1561 0.014
116 35.121 616 0.005
117 35.487 19190 0.182
118 35.892 2261 0.021 Limonen-10-ol
119 36.302 1551 0.014 Caryophyllene oxide
120 37.203 2536 0.024
121 37.660 3554 0.033 Humulene epoxide I
122 38.680 5605 0.053
123 38.888 7257 0.068 Humulene epoxide II
124 39.082 690 0.006 Caryolan-1-ol
125 40.154 1386 0.013 Humulene epoxide III
126 40.669 3261 0.031
127 40.872 1801 0.017
128 41.248 3412 0.032
129 41.399 1245 0.011 Humulol
130 41.937 4179 0.039
131 43.258 1708 0.016
Total area = 1.0527E + 007
______________________________________
Area Percent Report
Sample Name: 92-12-9-1
Pk # Ret Time Area Area %
Identification
______________________________________
1 7.028 3603 0.029
2 7.848 1142 0.009
3 8.004 772 0.006
4 9.048 3120 0.025
5 9.253 7251 0.060
6 9.351 5689 0.047 alpha Pinene
7 9.794 4017 0.033
8 10.302 1522 0.012
9 10.538 14530 0.120
10 11.090 761 0.006
11 11.231 52365 0.435 beta Pinene
12 11.886 700 0.005
13 12.412 3424969 28.463
Myrcene
14 12.648 2525 0.021
15 12.725 2971 0.024
16 13.048 23622 0.196
17 13.193 43283 0.359
18 13.268 169688 1.410 Methylbutyl Isobutyrate
19 13.576 16461 0.136 Limonene
20 13.794 2031 0.016
21 13.929 18137 0.150
22 14.311 4573 0.038
23 14.595 10186 0.084
24 14.873 11403 0.094
25 15.080 12395 0.103
26 15.413 700 0.005
27 15.501 899 0.007
28 15.700 2332 0.019
29 15.796 15536 0.129
30 16.002 9137 0.075
31 16.243 38778 0.322
32 16.787 5760 0.047
33 16.906 1286 0.010
34 17.425 608 0.005
35 17.676 114830 0.954
36 18.247 625 0.005
37 18.947 1621 0.013
38 19.175 28172 0.234
39 19.294 3311 0.027
40 19.463 9984 0.083
41 19.771 2700 0.022
42 19.947 1731 0.014
43 20.130 4426 0.036
44 20.732 2604 0.021
45 20.874 10809 0.089
46 21.044 2047 0.017
47 21.230 61271 0.509
48 21.533 14424 0.119
49 21.780 1521 0.012
50 21.843 956 0.007
51 22.211 2063 0.017
52 22.293 15179 0.126
53 22.443 32264 0.268
54 22.570 3366 0.028
55 22.756 106737 0.887
56 23.080 5302 0.044
57 23.356 6309 0.052
58 23.680 44440 0.369 Linalool
59 23.941 75567 0.628
60 24.170 15675 0.130
61 24.434 2150 0.017
62 24.524 1616 0.013
63 24.806 7293 0.060
64 25.028 2541 0.021
65 25.278 37278 0.309
66 25.346 31734 0.263
67 25.605 32557 0.270
68 25.881 90397 0.751
69 26.140 1145130 9.516 Caryophyllene
70 26.269 176130 1.463
71 26.486 9543 0.079
72 26.835 1812 0.015
73 26.988 22481 0.186
74 27.140 17473 0.145
75 27.353 42477 0.353 Farnesene?
76 27.473 5304 0.044
77 27.783 61915 0.514
78 28.035 24287 0.201
79 28.343 2545867 21.157
Humulene
80 28.547 315497 2.622
81 28.832 2334 0.019
82 28.945 5985 0.049
83 29.120 75042 0.623
84 29.300 116317 0.966
85 29.469 97160 0.807
86 29.682 182550 1.517 Muurolene
87 29.764 166698 1.385 Selinene
88 29.967 25357 0.210 Geranyl Acetate
89 30.169 7062 0.058
90 30.417 507237 4.215 delta Cadiene
91 30.630 323297 2.686 gamma Cadiene
92 30.879 5404 0.044
93 31.069 10371 0.086
94 31.155 5319 0.044
95 31.332 399851 3.323 Geranyl Isoburyrate
96 31.467 81272 0.675 Undecanone-2
97 31.617 7782 0.064
98 31.730 18703 0.155 Geraniol
99 31.890 7540 0.062
100 32.109 8984 0.074
101 32.223 86628 0.719
102 32.436 9265 0.077
103 32.491 6647 0.055
104 32.620 30270 0.251
105 33.035 3306 0.027
106 33.230 743 0.006
107 33.464 4214 0.035
108 33.728 87090 0.723
109 33.951 2444 0.020
110 34.183 3253 0.027
111 34.370 4703 0.039
112 34.480 4897 0.040
113 34.758 3342 0.027
114 34.896 11595 0.096
115 35.007 14221 0.118
116 35.299 1278 0.010
117 35.489 39880 0.331
118 35.873 5944 0.049 Limonen-10-ol
119 35.982 1196 0.009
120 36.163 3016 0.025
121 36.310 9199 0.076 Caryophyllene oxide
122 36.564 1474 0.012
123 36.871 5580 0.046
124 37.205 13593 0.113
125 37.365 21562 0.179
126 37.551 3095 0.025
127 37.669 39322 0.326 Humulene epoxide I
128 37.863 1467 0.012
129 38.004 7647 0.063
130 38.124 2074 0.017
131 38.438 1793 0.014
132 38.685 50800 0.422
133 38.900 79889 0.663 Humulene epoxide II
134 39.082 10671 0.088 Caryolan-1-ol
135 39.209 4042 0.033 Humulene epoxide III
136 39.500 2852 0.023
137 39.716 694 0.005
138 39.789 600 0.005
139 40.149 3072 0.025
140 40.465 1310 0.010
141 40.672 16151 0.134
142 40.874 13029 0.108
143 41.250 10136 0.084
144 41.406 45643 0.379 Humulol
145 41.758 11528 0.095
146 41.846 13183 0.109
147 41.945 25814 0.214
148 42.073 9818 0.081
149 42.534 5222 0.043
150 42.964 29878 0.248 beta Eudesmol?
151 43.065 24765 0.205 alpha Eudesmol?
152 43.255 33781 0.280
153 43.565 1828 0.015
154 43.846 10003 0.083
155 44.289 5485 0.045
156 44.370 3097 0.025
157 44.633 13527 0.112 Humulenol II
158 45.042 12525 0.104
159 45.700 38097 0.316 Humulene diepoxide?
160 45.885 3830 0.031
161 46.615 2140 0.017
162 47.030 2967 0.024
163 48.254 4136 0.034
164 48.524 1729 0.014
165 52.198 1275 0.010
Total area = 1.20327E + 007
______________________________________
Area Percent Report
Sample Name: 92-12-9-2
Pk # Ret Time Area Area %
Identification
______________________________________
1 7.034 1627 0.013
2 7.850 1222 0.010
3 9.051 1362 0.011
4 9.259 2214 0.018
5 9.355 9693 0.081 alpha Pinene
6 9.796 3741 0.031
7 10.309 1970 0.016
8 10.542 19685 0.166
9 11.093 943 0.008
10 11.236 79174 0.667 beta Pinene
11 11.895 2296 0.019
12 12.468 6160576 51.972
Myrcene
13 12.679 3749 0.031
14 12.741 4270 0.036
15 13.058 44822 0.378
16 13.201 59258 0.499
17 13.275 151104 1.274 Methylbutyl Isobutyrate
18 13.582 21713 0.183 Limonene
19 13.800 1814 0.015
20 13.934 28562 0.241
21 14.144 933 0.007
22 14.311 6730 0.056
23 14.519 2088 0.017
24 14.609 6243 0.052
25 14.875 55333 0.466
26 15.083 3970 0.033
27 15.326 1045 0.008
28 15.416 636 0.005
29 15.503 2028 0.017
30 15.702 2642 0.022
31 15.798 10210 0.086
32 16.005 54321 0.458
33 16.241 21435 0.180
34 16.809 7970 0.067
35 17.438 1939 0.016
36 17.655 61020 0.514
37 17.783 1252 0.010
38 18.248 665 0.005
39 18.948 1086 0.009
40 19.179 60530 0.510
41 19.314 1798 0.015
42 19.462 8464 0.071
43 19.546 4332 0.036
44 19.785 6451 0.054
45 19.949 1390 0.011
46 20.130 4840 0.040
47 20.722 11806 0.099
48 20.873 1283 0.010
49 20.945 2757 0.023
50 21.226 4405 0.037
51 21.524 2418 0.020
52 21.776 2520 0.021
53 22.199 1123 0.009
54 22.294 37518 0.316
55 22.438 12669 0.106
56 22.558 1478 0.012
57 22.743 43371 0.365
58 23.076 1222 0.010
59 23.236 1059 0.008
60 23.355 8797 0.074
61 23.681 121220 1.022 Linalool
62 23.935 17913 0.151
63 24.167 9930 0.083
64 24.417 1477 0.012
65 24.798 946 0.008
66 24.923 911 0.007
67 25.023 2303 0.019
68 25.347 50189 0.423
69 25.462 730 0.006
70 25.605 63484 0.535
71 25.731 14566 0.122
72 25.863 19067 0.160
73 26.129 1019106 8.597 Caryophyllene
74 26.267 172193 1.452
75 26.484 13509 0.114
76 26.649 3488 0.029
77 26.830 1518 0.012
78 26.973 2085 0.017
79 37.057 3159 0.026
80 27.127 4597 0.038
81 27.284 19285 0.162 Farnesene?
82 27.452 4597 0.038
83 27.558 1014 0.008
84 27.766 17735 0.149
85 28.030 20185 0.170
86 28.324 2255638 19.029
Humulene
87 28.509 62699 0.529
88 28.575 78837 0.665
89 28.933 16176 0.136
90 29.101 26411 0.222
91 29.319 43633 0.368
92 29.442 23012 0.194
93 29.658 109275 0.921 Muurolene
94 29.741 97039 0.818 Selinene
95 29.881 5248 0.044 Geranyl Acetate
96 30.142 4602 0.038
97 30.351 109776 0.926 delta Cadinene
98 30.580 52832 0.445 gamma Cadinene
99 30.771 1506 0.012
100 30.851 1737 0.014
101 31.082 5775 0.048
102 31.205 11532 0.097
103 31.330 9041 0.076 Geranyl isobutyrate
104 31.511 53799 0.453 Undecanone-2
105 31.717 9415 0.079 Geraniol
106 31.814 3048 0.025
107 32.085 5681 0.047
108 32.219 5853 0.049
109 32.492 1416 0.011
110 32.608 7265 0.061
111 33.033 906 0.007
112 33.253 722 0.006
113 33.712 10903 0.092
114 33.945 1308 0.011
115 34.071 2438 0.020
116 34.181 1069 0.009
117 34.368 5360 0.045
118 34.474 674 0.005
119 34.889 9616 0.081
120 35.001 4520 0.038
121 35.481 8543 0.072
122 35.853 4382 0.037 Limonen 10-ol
123 35.976 1266 0.010
124 36.154 1163 0.009
125 36.296 3788 0.032 Caryophyllene oxide
126 36.909 1692 0.014
127 37.183 9203 0.077
128 37.358 1754 0.014
129 37.660 26083 0.220 Humulene epoxide I
130 37.996 2405 0.020
131 38.673 14395 0.121
132 38.889 38989 0.328 Humulene epoxide II
133 39.079 1867 0.015 Caryolan-1 ol
134 39.922 885 0.007
135 40.138 1296 0.010
136 40.666 11481 0.096
137 40.870 4099 0.034
138 41.245 7744 0.065
139 41.397 4776 0.040 Humulol
140 41.934 9925 0.083
141 43.059 2394 0.020
142 43.254 5249 0.044
143 43.842 1927 0.016
144 44.637 2619 0.022 Humulenol II
= 1.18535E + 007
______________________________________
Reference:
Code Variety
92-12-8-2
Columbus
92-12-8-3
Galena
92-12-9-1
Chinook
92-12-9-2
Nugget
Analytical Conditions: See, Nickerson and van Engel, "Hop Aroma Component
Profile and the Aroma Unit," Journal American Society of Brewing Chemists
50(3), 7782(1992).
Claims
1. A new and distinct variety of hop plant named Columbus, substantially as shown and described.
- Defendant's First Motion for Partial Summary Judgment, dated Jun. 9, 1997. Defendants' Memorandum of Points and Authorities in Support of Their First Motion for Partial Summary Judgement, dated Jun. 9, 1997. Defendants' LR 56.1(a) Statement of Material Facts for Defendants' First Partial Summary Judgment Motion, dated Jun. 9, 1997 (without exhibits). Plaintiff's Statement of Specific Facts in Opposition to Defendants' Motion for Partial Summary Judgment, dated Jun. 25, 1997 (without exhibits) (information subject to protective order redacted). Plaintiff's Opposition to Defendants' First Motion for Partial Summary Judgment Motion, dated Jun. 25, 1997. HopUnion's Response in Opposition to Defendants' Motion for Partial Summary Judgment, dated Jul. 8, 1997 (without exhibits). Lewis, "The HopUnion U.S.A., Inc.--Low Trellis Hop Production System," Proc. Tech. Comm. of the IHGC of the XXXVIIIth International Hop Congress, Hereford, U.K. Published Rijksstation voor Landbouwtechniek, Merelbeke/Gent, Belgium, 17-37, 1990. Kenny, Identification of U.S. Grown Hop Cultivars by Hop Acid and Essential Oil Analyses, J. Amer. Soc. Brewing Chemists 48(1):3-8, 1990. Peacock and McCarty, "Varietal Identification of Hops and Hop Pellets," Master Brewer Assoc. of the Americas Technical Quarterly 27:81-85, 1992. Nickerson and van Engel, "Hop Aroma Component Profile and the Aroma Unit," Journal of American Society of Brewing Chemists 50(3), 77-82, 1992. American Society of Brewing Chemists, ".alpha.-Acids and .beta.-Acids in Hops and Hop Extracts by HPLC (International Method)," in "Methods of Analysis," The Society, St. Paul, MN, 8th ed., 1992. Hop Growers of America, Inc., 1991-1992 Annual Statistical Report, 37th Annual HGA Convention, Portland, Oregon, 1993.
Type: Grant
Filed: Mar 22, 1995
Date of Patent: Jun 15, 1999
Assignee: Hopunion USA (Yakima, WA)
Inventors: Gregory K. Lewis (Yakima, WA), Charles E. Zimmermann (Prosser, WA), Henry Hazenberg (Kennewick, WA)
Primary Examiner: Howard J. Locker
Law Firm: Seed and Berry LLP
Application Number: 8/409,908
