Method and apparatus for amperometric diagnostic analysis

The present invention relates to a novel method and apparatus for the amperometric determination of an analyte, and in particular, to an apparatus for amperometric analysis utilizing a novel disposable electroanalytical cell for the quantitative determination of biologically important compounds from body fluids.

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Claims

1. A method of measuring the amount of a selected compound in body fluids comprising,

a) providing a measuring cell having at least a first and second electrode and said cell containing an oxidant and a buffer,
b) placing a sample of fluid to be tested in said cell,
c) reconstituting said oxidant and buffer with said sample fluid to generate a predetermined reaction,
d) allowing said reaction to proceed substantially to completion,
e) applying a potential across said electrodes and sample, and
f) measuring the resulting Cottrell current to determine the concentration of said selected compound present in said sample.

2. A method as set forth in claim 1, wherein the compound is selected from the group consisting of glucose, cholesterol, TSH, T4, hormones, antiarrhythmics, antiepileptics and nontherapeutic drugs.

3. A method as set forth in claim 1, wherein the oxidant is a material selected from the group consisting of benzoquinone, ferricyanide, ferricinium,.Iadd.orthophenanthroline.Iaddend.and Cobalt (III) dipyridyl.

4. The method as set forth in claim 1 including providing

as said first electrode a working electrode and as said second electrode a reference electrode.
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Patent History
Patent number: RE36268
Type: Grant
Filed: Jul 12, 1996
Date of Patent: Aug 17, 1999
Assignee: Boehringer Mannheim Corporation (Indianapolis, IN)
Inventors: Neil J. Szuminsky (Pittsburgh, PA), Joseph Jordan, deceased (late of State College, PA), Paul A. Pottgen (Allison Park, PA), Jonathan L. Talbott (Freedom, PA)
Primary Examiner: Bruce F. Bell
Attorney: D. Michael Young
Application Number: 8/679,312
Classifications
Current U.S. Class: 205/7775; 205/7825; Three Or More Electrodes (204/412); Selectively Permeable Membrane (204/415); 435/2871; 435/2891; Enzyme Or Microbe Electrode (435/817)
International Classification: G01N 2726;