Abstract: A process for preparing an aqueous dispersion of a silicone resin is disclosed by reacting a mixture containing an alkylalkoxysilane and a cationic alkoxysilane to form a self dispersing silicone resin and subsequently combining the silicone resin with water to form the dispersion. The resulting dispersions are useful for forming coatings on various substrates that are essentially free of co-surfactants or volatile organic solvents.

Abstract: Provided is a film comprising a polymer that comprises a structural unit represented by the following formula (1), wherein at least part of the terminal structure of the polymer is at least one structure selected from the group consisting of structural units represented by the following formula (2) and structural units represented by the following formula (3).

Abstract: The present invention provides a method of manufacturing polyamide-carbon nanotube composites. The method includes mixing a polyamide composition including 0.01-1% by weight of carbon nanotubes using a shearing rate equal to or greater than 1000-4400 sec?1.

Abstract: A process for obtaining an aqueous emulsion comprising a block copolymer by the solution polymerisation of vinyl monomers to obtain block [B] in the presence of a) a radical precursor; and b) an iodine atom containing block [A]; where block [A] and [B] together comprise 0 to 2 wt % of methacrylic acid; where block [A] and block [B] together comprise ?2.5 wt % of vinyl monomers bearing ionic or potentially ionic water-dispersing groups not including methacrylic acid; and performing a post polymerisation reaction on the block copolymer emulsion obtained in step II; and wherein said aqueous emulsion has a free vinyl monomer level <1000 ppm when having a solids content of ?20 wt %.

Abstract: The present invention discloses a gene carrier and the preparation method thereof. Chondroitin sulfate (CS) is reacted with methacrylic anhydride (MA) to form chrondroitin sulfate-methacrylate (CSMA), which is further covalently bound with polyethylenimine (PEI) via the Michael addition to produce a CSMA-PEI gene carrier. The CSMA-PEI gene carrier can effectively reduce the cytotoxicity of PEI and enhance the transfection efficiency of PEI.

Abstract: The present invention relates to polymers, nanomaterials, and methods of making the same. Various embodiments provide an amphiphilic multi-arm copolymer. The copolymer includes a core unit and a plurality of amphiphilic block copolymer arms. Each block copolymer arm is substituted on the core unit. Each block copolymer arm includes at least one hydrophilic homopolymer subunit and at least one hydrophobic homopolymer subunit. In some examples, the copolymer can include a star-like or bottlebrush-like block copolymer, and can include a Janus copolymer. Various embodiments provide a nanomaterial. In some examples, the nanomaterial can include Janus nanomaterials, and can include nanoparticles, nanorods, or nanotubes. The nanomaterial includes the amphiphilic multi-arm copolymer and at least one inorganic precursor. The inorganic precursor can be coordinated to at least one homopolymer subunit of one of the amphiphilic block copolymer arms to form the nanomaterial.

Abstract: The invention is a novel family of polyolefins characterized by chain-walking defects of the type that add extra backbone carbons per monomer. These polyolefins display a large decrease in crystallinity relative to polyolefins known in the art. Specifically, the reduction in crystallinity is much greater than for earlier polypropylenes with a matched content of stereo or 1-alkene type defects. The claimed polyolefins can be an alkene-based homopolymer. The defects in the polyolefin backbone are generated by a chain walking mechanism in which three or more carbons per monomer are added to the polymer backbone instead of two, as in conventional polymerization or copolymerization methods of alpha olefins. The novel polyolefins can be used in applications such as plastic wrapping, thin films, co-extrusion layers or molded parts in the absence of polymer blending or copolymerization. The cost of materials production can be reduced.

Abstract: A composition is provided, which comprises chains comprising a first graft copolymer of a first elastomer and a poly(L-lactic acid), and chains comprising a second graft copolymer of a second elastomer and a poly(D-lactic acid). At least some of the poly(L-lactic acid) and poly(D-lactic acid) crosslink the chains. Poly(L-lactic acid) and poly(D-lactic acid) may form stereocomplexes that crosslink the chains. The chains may be crosslinked by crystalline structures formed from at least some of the poly(L-lactic acid) and poly(D-lactic acid) in discrete regions. The crosslinked chains may form a matrix. In a method of forming the composition, the first and second graft copolymers are mixed, such as by melt blending or solution casting, to form the composition. The graft copolymers may be formed by a “grafting-though” or “grafting-from” process. The composition may be useful under a relatively wide range of temperatures.

Abstract: Provided is an artificial turf yarn having improved heat resistance, durability, softness and extensibility. The artificial turf yarn contains two components: an olefin block copolymer (OBC) and a linear low density polyethylene (LLDPE). The yarn includes from about 10 wt % to about 80 wt % of the OBC and from about 20 wt % to about 90 wt % of the LLDPE which produces an artificial turf yarn with improved softness and toughness while maintaining heat resistance.

Abstract: A thermoplastic elastomer composition having a continuous phase and a dispersed phase, wherein the continuous phase comprising (A) an epoxy-modified polyamide resin, and the dispersed phase comprising (B) a halogenated isoolefin-paraalkylstyrene copolymer rubber, wherein the thermoplastic elastomer composition is obtained by melt-kneading (B) halogenated isoolefin-paraalkylstyrene copolymer rubber, (C) a polyamide resin, and (D) a polyfunctional epoxy compound having two or more epoxy groups per molecule in an amount of 0.05 parts by weight or more and less than 3 parts by weight with respect to 100 parts by weight of polyamide resin (C), at a temperature which is equal to or more than the melting point of polyamide resin (C), and epoxy-modified polyamide resin (A) is produced by the reaction of polyamide resin (C) with polyfunctional epoxy compound (D) during the melt-kneading.

Abstract: A polyethylene moulding composition having a multimodal molecular weight distribution, having a density according to ISO 1183 at 23° C. in the range of 920 to 960 kg/m3 and an MFR190/2 according to ISO 1133 in the range of 0.

Abstract: This invention relates to a process to functionalize propylene co-oligomer comprising contacting an alkene metathesis catalyst with a heteroatom containing alkene, and a propylene a co-oligomer having an Mn of 300 to 30,000 g/mol comprising 10 to 90 mol % propylene and 10 to 90 mol % of ethylene, wherein the oligomer has at least X % allyl chain ends, where: 1) X=(?0.94 (mol % ethylene incorporated)+100), when 10 to 60 mol % ethylene is present in the co-oligomer, and 2) X=45, when greater than 60 and less than 70 mol % ethylene is present in the co-oligomer, and 3) X=(1.83*(mol % ethylene incorporated)?83), when 70 to 90 mol % ethylene is present in the co-oligomer.

Abstract: The present invention provides a styrene-based star polymer with narrow dispersion and suitable as a resist material, etc. A star polymer of the present invention is represented by the formula A[C(Y)Xm]n (wherein A represents a polyvalent aliphatic hydrocarbon group with 4-15 carbons, C represents a carbon atom, X represents a styrene-based polymer chain, Y represents a hydroxy group or an oxo group, m represents an integer of 1 or 2, and n represents any integer from 2 to 5, with the proviso that if Y is a hydroxy group, m is 2, and if Y is an oxo group, m is 1). The star polymer can be produced by reacting a styrene-based polymer having an anionic end, and an aliphatic carboxylic acid ester represented by A(COOR)n (wherein R represents an alkyl group having 1-8 carbons, and A and n are as defined for the above formula).

Abstract: The brittleness of a thermoplastic material containing a polylactic acid may be reduced by melting and mixing the thermoplastic material and a graft copolymer to link the graft copolymer to thermoplastic polymer to form a new thermoplastic material with reduced brittleness. The graft copolymer comprises an elastomeric backbone and a side chain grafted to the backbone. The side chain comprises a enantiomer of lactic acid opposite to the enantiomer in the thermoplastic material. A composition comprises a thermoplastic polymer and the graft copolymer, where the graft copolymer is linked to the thermoplastic polymer by the enantiomers. A method of forming the composition may comprise melting precursors for the thermoplastic polymer and the graft copolymer, and mixing the precursors to allow the lactic acids to link the graft copolymer to the thermoplastic polymer.

Abstract: Disclosed is a method for manufacturing a polylactic acid film. More specifically, the present invention relates to a method for stably and effectively obtaining optimized stereocomplex crystals through specified boiling point and evaporation time of a solvent allowing the content of stereocomplex crystals to be 100%.

Abstract: A method for producing block polymers which involves (i) a step for obtaining a polymer of first monomers by means of a first polymerization reaction to polymerize the first monomers in the presence of an alkoxyamine catalyst, and (ii) a step for obtaining a block polymer by means of a second polymerization reaction to polymerize second monomers in the presence of an alkoxyamine catalyst and the polymers of the first monomers, wherein at least one of the kinds of monomers is a fluorinated (meth)acrylate monomer.

Abstract: Silicone-polyether block copolymers are disclosed, having the average formula: R4R12Si(OR52Si)m{[R2O(CqH2qO)pR2R12Si(OR52Si)k]nR2O(CqH2qO)pR2R12Si)m}hOSiR12R4 wherein R1 is C1-C10 hydrocarbyl; R2 is C1-C10 hydrocarbylene; R3 is R1 or H; R4 is —R2—NR32 or —R2—NR3—R2—NR32; R5 is R1 or R4; h is 1 to 100; k is 2 to 500; m is 2 to 500; n is 0 to 30; p is 2 to 120; and q is 2 to 4. The silicone-polyether block copolymers comprise two terminal amine functionalities and may comprise varying degrees of pendent amine functionality. In a method for synthesizing linear silicone-polyether block copolymers of the above formula, a reaction mixture is formed by combining a polyether, an organosilicon component, an endblocking agent, and a catalyst. Thereupon, the reaction mixture is heated to produce a silicone-polyether block copolymer having at least one pendent amine functionality and a number-average molecular weight from 2000 to 500,000.

Abstract: The invention relates to a process for preparing polyolefin in a loop reactor. The polymer is prepared by polymerizing olefin monomers in the presence of a catalyst to produce a polyolefin slurry while pumping said slurry through said loop reactor by means of a pump. The present process is characterized in that the catalyst is fed in the loop reactor at a distance to the pump. The invention allows production of the polymer with advantageous properties while leading to fewer blockages of the reactor.

Abstract: A process for producing functional polymer, the process comprising the steps of reacting an anionic initiator including a functional group with a stabilizing monomer to produce a stabilized initiator, reacting the stabilized initiator with monomer capable of being anionically polymerized to produce a polymer, and terminating the polymer.

Abstract: The various embodiments herein disclose a method for making a Zieglaer catalyst including a reaction product of a dispersed magnesium alkoxide or a dispersed mixture of magnesium alkoxide and silica with a transition metal compound of titanium, zirconium, vanadium or chromium, and a chlorine containing organoaluminum mixed together with two additional components comprising a halogen containing silicon compound and aliphatic primary halogenated hydrocarbons respectively. The embodiments also provide a method of polymerization of 1-olefin monomers using the Ziegler catalyst.

Abstract: Vinyl-terminated macromonomer oligomerization, namely, a process to produce polymacromonomers comprising contacting a vinyl-terminated macromonomer with a catalyst system capable of oligomerizing vinyl-terminated macromonomer, in the presence of an aluminum containing compound, a zinc containing compound, or a combination thereof, under polymerization conditions to produce a polymacromonomer, and polymacromonomers produced thereby. Also, polymacromonomers having a degree of polymerization greater than 10, a glass transition temperature Tg of less than 60° C., and an Mn of greater than or equal to about 5000 Da.

Abstract: Disclosed is an optical material including a cyclic olefin copolymer containing at least a repeating structural unit [A] represented by the general formula (1) and a repeating structural unit [B] represented by the general formula (2) and having a molar ratio [A]/[B] of from 95/5 to 1/99, wherein the absolute value of the ratio ?OB/?F is from 0.001 to 0.250 in which ?OB represents the amount of a change in the absolute value of the orientation birefringence calculated from the phase difference (nm) at a wavelength of 633 nm/film thickness (?m) in a stretched film obtained from the optical material, and ?F represents the amount of a change in the absolute value of the orientation coefficient determined from the dichroic ratio of parallel light intensity I? to perpendicular light intensity I?, each relative to the orientation direction according to Raman spectroscopy.

Abstract: A propylene/ethylene/1-hexene terpolymer containing from 1.0 wt% to 2.5 wt %, of ethylene derived units and from 2.0 wt % to 4.0 wt % of 1-hexene derived units, the sum of the amounts of propylene, ethylene and 1-.hexene derived units being 100, having the following features: a) the amount (Wt %) of 1-hexene (C6) and the amount (Wt %) of ethylene (C2) fulfil the following relation: C2<?0.18C6+2.86; b) Polydispersity index (PI) ranging from 2 to 10; c) melting temperature ranging from 125° C., to 139° C.; d) solubility in xylene at 25° C. below 10 wt %; e) melt index (MFR) of from 4 to 6 g/10? dg/min, according to ISO method 1133; and f) crystallization temperature ranging from 85° C. to 100° C.

Abstract: The present invention relates to a method for producing an organopolysiloxane compound having a structure in which a poly(N-acylalkylene imine) segment containing a repeating unit represented by the following general formula (1) is bonded to a terminal end and/or a side chain of an organopolysiloxane segment, the method including the steps of (a) subjecting a cyclic iminoether compound represented by the following general formula (I) to ring opening polymerization in a solvent to prepare a solution of a terminal-reactive poly(N-acylalkylene imine); (b) mixing a modified organopolysiloxane containing an amino group bonded to a terminal end and/or a side chain of a molecular chain thereof with a solvent to prepare a solution of the modified organopolysiloxane; (c) mixing the terminal-reactive poly(N-acylalkylene imine) solution prepared in the step (a) with the modified organopolysiloxane solution prepared in the step (b) to react the amino group contained in the modified organopolysiloxane with the terminal-re

Abstract: A dendritic polymer and a magnetic resonance imaging contrast agent employing the same. The magnetic resonance contrast agent includes the dendritic polymer according to the structure of S?P-D?Z-L)i)j or S?P-D?X—Z-L)i)j, wherein, S is cyclosilane moiety with j silicon oxygen residual groups, and j is not less than 2; P is ?CH2CH2O?l, and l is not less than 1; D is a C3-30 dendritic moiety having n oxygen residue, and n is not less than 3; X is C3-30 moiety having bi-functional groups; Z is a C3-20 moiety having a plurality of functional group; and L is a metal cation.

Abstract: A polyol (PL) for polyurethane preparation is disclosed that includes the polyol (a) and the strength-enhancing agent (b), as follows. Polyol (a): a polyoxyalkylene polyol that is the alkylene oxide adduct of an active hydrogen-containing compound (H), in which at least 40% of a hydroxyl group positioned on the terminal is a primary hydroxyl group-containing group represented by general formula (I). In general formula (I), R1 represents a hydrogen atom or an alkyl group having 1 to 12 carbon atoms, cycloalkyl group, or phenyl group. Strength-enhancing agent (b): a compound that is at least one compound selected from the group comprising an ester compound, a thioester compound, a phosphoric acid ester compound, and an amide compound, and that is derived from an aromatic polyvalent carboxylic acid with a valence of 2 or higher.

Abstract: The present invention provides a method for producing a liquid-crystalline polyester, which comprises melt-polymerizing monomers in a reactor having a draw outlet to obtain a polymer melt and drawing the polymer melt through the draw outlet, characterized in that the monomers comprise a compound selected from an aromatic hydroxycarboxylic acid and derivatives thereof, a compound selected from an aromatic dicarboxylic acid and derivatives thereof and a compound selected from an aromatic diol, an aromatic hydroxyamine, an aromatic diamine and derivatives thereof; the amount of units derived from a compound containing a 1,2-phenylene and/or a 1,3-phenylene skeleton(s) in the polyester is from 0 to 10 mold; the melt polymerization is performed in the presence of a heterocyclic compound containing two or more nitrogen atoms; and the polymer melt has a flow initiation temperature of from 220 to 250° C.

Abstract: The present invention provides a method for producing a liquid-crystalline polyester, the method comprising: a step of melt-polymerizing raw monomers in a melt polymerization vessel to obtain a polymer melt; a step of drawing the polymer melt from the melt polymerization vessel and granulating the polymer melt to obtain a granulate; a step of allowing the granulate to undergo solid phase polymerization in a solid phase polymerization vessel to obtain a solid phase polymer; and a step of making an impact on the solid phase polymerization vessel containing the solid phase polymer, and taking out the solid phase polymer from the solid phase polymerization vessel.

Abstract: A method for manufacturing a liquid crystal polyester, comprising: a melt polycondensation step in which 0.001 to 1% by mass of a heterocyclic aromatic compound represented by the following formula (I) is added to a monomer mixture comprising at least one monomer selected from the group consisting of terephthalic acid, a terephthalic acid derivative, 2,6-naphthalenedicarboxylic acid and a 2,6-naphthalenedicarboxylic acid derivative, relative to 100% by mass of the monomer mixture, and then a melt polycondensation is conducted at a temperature of 240 to 300° C. so as to obtain a polymer. (wherein each of X1 and X2 independently represents a methyl group, an ethyl group, a propyl group, a butyl group or a pentyl group, provided that one or more hydrogen atoms on a heterocyclic aromatic ring may each independently be replaced by a halogen atom, an alkyl group or an aryl group).

Abstract: Disclosed is a method for synthesizing star polymers having controlled tacticity. The method comprises reacting a lactone-based monomer having at least one stereocentre and a poly-functional initiator in the presence of at least one catalyst. In particular embodiments, the lactone-based monomer is L-lactide or DL-lactide, the poly-functional initiator is dipentaerythritol, and the at least one catalyst is an aluminum-salen catalyst (tBu[O,N,N,O] AlMe) or an aluminum-salan catalyst (Cl[O,N,N,O] AlMe). Star polymers comprising at least two arms and having heterotacticity bias, isotacticity bias, or stereoblocks of different tacticity bias are produced.

Abstract: The present invention provides a resin capable of contributing greatly to solve environmental problems and problems related to exhaustion of fossil fuel resources and having physical properties suited for practical use. The polyester according to the present invention has a diol and a dicarboxylic acid as constituent components and has an amount of terminal acid of 50 equivalents/metric ton or less.

Abstract: The invention relates to a method for preparing caprolactam comprising recovering a mixture containing 6-aminocaproic acid, from a culture medium comprising biomass, and thereafter cyclising the 6-aminocaproic acid in the presence of superheated steam, thereby forming caprolactam, wherein the weight to weight ratio carbohydrate to 6-aminocaproic acid in said mixture is 0.03 or less.

Abstract: Disclosed is a technique of providing color to a polylactic acid resin which is promising as a plant-derived material for automotive interior/exterior parts. When a polylactic acid material is used as a covering fiber material of an automotive interior part, coloring is necessary for harmonization with other parts in the car. According to the disclosed technique, color is provided to a polylactic acid resin using a combination of a catalyst and an initiator when it is polymerized from lactide. The disclosed method is time-saving and economical since a colored resin can be polymerized and thus the complicated process of dyeing a white resin using a disperse dye and washing and drying the same can be avoided.

Abstract: The present invention relates to a process for the large-scale synthesis of (Aib8,35)hGLP-1(7-36)-NH2 (SEQ ED NO:2), i.e., His-Aib-Glu-Gly-Thr-Phe-Thr-Ser-Asp-Val-Ser-Ser-Tyr-Leu-Glu-Gly-Gb-Ala-Ala-Lys-Glu-Phe-Ile-Ala-Trp-Leu-Val-Lys-Aib-Arg-NH2 (SEQ ID NO:2), which comprises solid-phase Fmoc-chemistry.

Abstract: The process described herein discloses purification process of a secondary metabolite produced by fermentation route. The process involves selective removal of impurities at various stages of washings, charcoalization followed by crystallization. The product is closely related to class of echinocandins and is found to be potent antifungal compound & a key ingredient in the synthesis of antifungal drugs.

Abstract: A purification method of the compound represented by formula 1 is provided, which includes the following steps: (1) loading crude compound 1 on macroporous adsorbent resin, (2) washing the macroporous adsorbent resin with an aqueous solution, an organic solvent or a mixture solution of organic solvent and water, (3) eluting with an aqueous solution, an organic solvent or a mixture solution of organic solvent and water. The purified compound represented by formula 1 is obtained.

Abstract: The present invention generally relates to methods and compositions for generating vancomycin analogs. Specifically the invention relates to generating a vancomycin library through chemoselective ligation of a sugar moiety with a vancomycin aglycon. In particular, the present invention provides a library of vancomycin analogs, where the member of the library comprises at least one vancomycin analog selected from 2?-N-acyldecanoyl-glucosyl vancomycin neoglycoside, 3?-N-acyldecanoyl-glucosyl vancomycin neoglycoside, 4?-N-acyldecanoyl-glucosyl vancomycin neoglycoside, 6?-N-acyldecanoyl-glucosyl vancomycin neoglycoside, 2?-N-acylbiphenyl-glucosyl vancomycin neoglycoside, 3?-N-acylbiphenoyl-glucosyl vancomycin neoglycoside, 4?-N-acylbiphenoyl-glucosyl vancomycin neoglycoside and 6?-N-acylbiphenoyl-glucosyl vancomycin neoglycoside.

Abstract: Target and method for inhibition of bacterial RNA polymerase disclosed are targets and methods for specific binding and inhibition of RNAP from bacterial species.

Abstract: Degradable bioprostheses made of collagen-based material having amine-based and ester-based crosslinks are provided, as are methods for their formation and use. Some embodiments of the present invention are directed towards a method of controlling the ratio of amine-based crosslinks to ester-based crosslinks within a collagen-based material to provide a tailorably crosslinked collagen-based material. Some embodiments provide a method of making a degradable bioprosthesis involving controlling crosslinking to afford a degradable bioprosthesis that is partially crosslinked. By controlling the ratio of amine-based to ester-based crosslinks, by controlling the level of crosslinking, or by controlling both of these features, degradable bioprostheses with tailored degradation rates can be synthesized. Some embodiments of degradable bioprostheses have degradation rates that are tailored to allow their use in particular medical applications.

Abstract: Substrates comprising a solid support, a ligand, and a linker comprising at least one C, O, N, or S atom covalently connecting the solid support to the ligand, are disclosed, along with methods of using and making the substrates, and devices including the substrates.

Abstract: The present invention relates to compositions and methods for characterizing, diagnosing, and treating cancer. In particular the invention provides the means and methods for the diagnosis, characterization, prognosis and treatment of cancer and specifically targeting cancer stem cells. The present invention provides an antibody that specifically binds to a non-ligand binding region of the extracellular domain of a human NOTCH receptor and inhibits growth of tumor cells. The present invention further provides a method of treating cancer, the method comprising administering a therapeutically effective amount of an antibody that specifically binds to a non-ligand binding region of the extracellular domain of a human NOTCH receptor protein and inhibits growth of tumor cells.

Abstract: Disclosed herein are antibodies and ILT5-binding fragments thereof that specifically bind to ILT5, e.g., human ILT5 (hILT5), and pharmaceutical compositions comprising such ILT5-binding antibodies and ILT5-binding fragments thereof.

Abstract: The present invention provides synthetic rodent antibody libraries, such as mouse or rat antibody libraries, as well as polypeptides, nucleic acids, vectors, host cells and methods used in conjunction with these libraries. The present invention also provides antibodies isolated from such libraries and variants of such antibodies.

Abstract: An antibody comprising an antigen recognition region which comprises CDR amino acid sequences set forth in SEQ ID NO: 7, 8, 9, 10, 11 and 12.

Abstract: The disclosure provides compositions and methods relating to or derived from anti-activin A binding proteins, including antibodies. In particular embodiments, the disclosure provides fully human, humanized, and chimeric anti-activin A antibodies that bind human activin A, activin A-binding fragments and derivatives of such antibodies, and activin A-binding polypeptides comprising such fragments. Other embodiments provide nucleic acids encoding such antibodies, antibody fragments and derivatives and polypeptides, cells comprising such polynucleotides, methods of making such antibodies, antibody fragments and derivatives and polypeptides, and methods of using such antibodies, antibody fragments and derivatives and polypeptides, including methods of treating or diagnosing subjects having activin A-related disorders or conditions including cachexia related to gonadal cancer, other cancers, rheumatoid arthritis, and other diseases.

Abstract: Disclosed herein are non-natural amino acids and polypeptides that include at least one non-natural amino acid, and methods for making such non-natural amino acids and polypeptides. The non-natural amino acids, by themselves or as a part of a polypeptide, can include a wide range of possible functionalities, but typical have at least one oxime, carbonyl, dicarbonyl, and/or hydroxylamine group. Also disclosed herein are non-natural amino acid polypeptides that are further modified post-translationally, methods for effecting such modifications, and methods for purifying such polypeptides. Typically, the modified non-natural amino acid polypeptides include at least one oxime, carbonyl, dicarbonyl, and/or hydroxylamine group. Further disclosed are methods for using such non-natural amino acid polypeptides and modified non-natural amino acid polypeptides, including therapeutic, diagnostic, and other biotechnology uses.

Abstract: Procedure for obtaining a composition that contains growth factors, which comprises the steps of heat-treating a platelet-rich plasma or the supernatant of a platelet-rich plasma that contains released growth factors in order to increase its temperature, for eliminating the complement and reducing the immunoglobulins present therein, and lyophilizing the plasma or supernatant in order to obtain a final dry composition that can easily be transported, handled and stored, thereby facilitating periodic or chronic treatments with blood compounds. It has been shown that when the final dry composition is resuspended, a once again humid composition is obtained that maintains its biological properties intact.

Abstract: A new process for extracting and purifying glucosinolates from plant material, preferably broccoli sprouts or seeds is described. An alcoholic extract is adsorbed onto a basic resin and eluted with ammonia. Optionally, the alcoholic extract is passed through an ion-exchange column containing acidic resin prior to the adsorption/elution step.

Abstract: The present invention relates to a method for isolating and purifying nucleic acids, preferably comprising genomic DNA, from biological samples comprising the steps of lysing the sample using a lysis buffer comprising a source of anionic surfactant ions, optionally disintegrating the RNA present in the lysate, precipitating the surfactant ions from the lysate, and separating the nucleic acids from the precipitate and further contaminants by size-exclusion chromatography. The invention furthermore relates to a lysis buffer, a method of lysing cells and a kit for the isolation and purification of nucleic acids.

Abstract: Water-forming NADH oxidase derived from Streptococcus mutans should be further improved in terms of stability for practical use in industrial production. An object of the present invention is to provide an enzyme that is obtained through modification of a water-forming NADH oxidase, which is useful as an NAD+ regeneration system for stereoselective oxidation catalyzed by an oxidoreductase, by protein engineering techniques so that the enzyme can withstand long-term use without exhibiting a reduction of its activity for the regeneration of NAD+, that is, an enzyme having improved stability, and to provide a method for efficiently producing a useful substance such as an optically active alcohol or amino acid. The present invention relates to an enzyme modification method that can improve the stability of water-forming NADH oxidase derived from Streptococcus mutans by appropriately introducing mutation.