Patents Issued in May 27, 2014
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Patent number: 8735030Abstract: Disclosed is a method of modifying of a surface of a substrate of a photolithographic mask for extreme ultraviolet radiation comprising the step of focusing femtosecond light pulses of a laser system onto the substrate so that a plurality of color centers is generated inside the substrate, wherein the color centers are distributed to cause a modification of the substrate surface.Type: GrantFiled: April 12, 2011Date of Patent: May 27, 2014Assignees: Carl Zeiss SMT GmbH, Carl Zeiss SMS GmbH., Carl Zeiss SMS LtdInventors: Sergey Oshemkov, Ralph Klaesges, Markus Mengel
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Patent number: 8735031Abstract: An electrophotographic photoreceptor includes an electroconductive substrate; an undercoat layer provided on the electroconductive substrate and composed of: metal oxide fine particles including particles of at least one metal oxide and at least one organic compound provided on the particles of the at least one metal oxide as a surface treatment; and a copolymer resin synthesized by copolymerization of essential constituent monomers composed of a dicarboxylic acid, a diol, a triol and a diamine; and a photosensitive layer laminated on the undercoat layer. The undercoat layer permits (a) attaining stable electric potential characteristics in all environments ranging from low temperature and low humidity environments to high temperature and high humidity environments, (b) suppressing the occurrence of printing defects and density differences, and (c) simultaneously attaining transfer restorability and restorability from intense light-induced fatigue even in a wide variety of usages and operation environments.Type: GrantFiled: November 27, 2009Date of Patent: May 27, 2014Assignee: Fuji Electric Co., Ltd.Inventors: Kazuki Nebashi, Yoichi Nakamura, Ikuo Takaki, Seizo Kitagawa, Shinjiro Suzuki
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Patent number: 8735032Abstract: An electrophotographic photosensitive member having a surface layer that contains a polymer produced by the polymerization of a compound having a chain-polymerizable functional group. The compound having a chain-polymerizable functional group is a compound represented by the formula (1).Type: GrantFiled: May 22, 2012Date of Patent: May 27, 2014Assignee: Canon Kabushiki KaishaInventors: Masaki Nonaka, Masato Tanaka
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Patent number: 8735033Abstract: A process for making emulsion aggregation (EA) toners is provided. In embodiments, the process comprises aggregating a mixture comprising a latex resin, and at least one colorant in a reactor to form aggregated toner particles, adding a shell resin to form a shell over the aggregated toner particles, coalescing the aggregated toner particles, and recovering the toner particles.Type: GrantFiled: March 29, 2012Date of Patent: May 27, 2014Assignee: Xerox CorporationInventors: Yulin Wang, Kimberly Nosella, David Kurceba, Santiago Faucher
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Patent number: 8735034Abstract: A toner for electrostatic image development containing toner matrix particles having at least a resin binder and a colorant, and an external additive, the external additive having composite oxide particles (an external additive A) that have titania and silica, wherein the external additive A has a core-shell structure in which a core portion has the titania and a shell portion has the silica, wherein the titania is present in the external additive A in an amount of from 75 to 95% by weight, and the resin binder comprising a polyester obtained by polycondensing a carboxylic acid component comprising an isophthalic acid compound and an alcohol component (a polyester A).Type: GrantFiled: December 2, 2011Date of Patent: May 27, 2014Assignee: Kao CorporationInventors: Masahito Yamazaki, Satoshi Kunii
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Patent number: 8735035Abstract: An electrophotographic toner and a process for preparing the same. The electrophotographic toner includes a binder, a coloring agent, and a release agent. The binder includes two resins having different weight average molecular weights.Type: GrantFiled: January 20, 2012Date of Patent: May 27, 2014Assignee: Samsung Electronics Co., Ltd.Inventors: Ju-yeon Lee, Jun-young Lee, Kyeong Pang, Tae-hoe Koo, Su-bum Park
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Patent number: 8735036Abstract: An electrostatic charge image developing toner includes: toner particles; and an external additive having an average particle diameter of from about 70 nm to about 420 nm, an average circularity of equal to or less than about 0.9, and a standard deviation of the circularity of greater than 0.2.Type: GrantFiled: March 28, 2012Date of Patent: May 27, 2014Assignee: Fuji Xerox Co., Ltd.Inventors: Katsuyuki Kitajima, Yutaka Saito, Sakon Takahashi, Michio Take
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Patent number: 8735037Abstract: A toner obtained by a method for producing a toner, which includes dissolving or dispersing in an organic solvent a toner material containing at least a binder resin, and a dispersion liquid of a crystalline polyester resin, so as to prepare a solution or dispersion liquid of the toner material, emulsifying or dispersing the solution or dispersion liquid of the toner material in an aqueous medium, so as to prepare an emulsion or dispersion liquid, and removing the organic solvent from the emulsion or dispersion liquid, wherein the crystalline polyester resin is localized near a surface of the toner.Type: GrantFiled: March 16, 2011Date of Patent: May 27, 2014Assignee: Ricoh Company, Ltd.Inventors: Hiroshi Yamashita, Masaki Watanabe, Tomoki Murayama
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Patent number: 8735038Abstract: A toner for non-contacting fusing containing toner matrix particles containing a resin binder and an external additive having an average particle size of from 10 to 100 nm, wherein the external additive is externally added to the toner matrix particles, wherein the resin binder contains one or more polyesters, wherein a carboxylic acid component of the polyester contains one or more isophthalic acid compounds and one or more fumaric acid/maleic acid compounds, wherein the isophthalic acid compound is contained in an amount of from 10 to 35% by weight, the fumaric acid/maleic acid compound is contained in an amount of from 1 to 15% by weight, and the isophthalic acid compound and the fumaric acid/maleic acid compound are contained in a total amount of from 20 to 36% by weight, of a total amount of the entire raw material monomers of the polyester in the resin binder, and wherein the toner has a softening point of from 90° to 120° C.Type: GrantFiled: October 3, 2012Date of Patent: May 27, 2014Assignee: Kao CorporationInventors: Satoshi Kunii, Masahito Yamazaki, Takashi Kubo
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Patent number: 8735039Abstract: A toner for electrostatic image development containing at least a resin binder, the resin binder being composed of a crystalline resin and an amorphous resin, wherein the crystalline resin contains a crystalline polyester obtained by polycondensing an alcohol component containing an aliphatic diol having 2 to 10 carbon atoms and a carboxylic acid component containing an aromatic dicarboxylic acid compound and an aromatic tricarboxylic or higher polycarboxylic acid compound, wherein the crystalline polyester has a weight-average molecular weight of from 20,000 to 150,000, and wherein the crystalline resin and the amorphous resin are in a weight ratio, i.e. crystalline resin/amorphous resin, of from 55/45 to 95/5. The toner for electrostatic image development of the present invention can be suitably used in, for example, the development or the like of latent image formed in electrophotography, an electrostatic recording method, an electrostatic printing method, or the like.Type: GrantFiled: November 9, 2012Date of Patent: May 27, 2014Assignee: Kao CorporationInventor: Yasuhiro Hidaka
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Patent number: 8735040Abstract: To provide a toner including at least a binder resin and a colorant, wherein the binder resin has two glass transition temperatures Tg1 and Tg2 in a differential scanning calorimetry at a heating speed of 5° C./min, the glass transition temperature Tg1 is ?20° C. to 20° C., and the glass transition temperature Tg2 is 35° C. to 65° C., wherein the binder resin comprises a polyester skeleton and a ring-containing skeleton molecule at ends thereof, and wherein the binder resin is obtained by block copolymerization of: a polyester skeleton A which includes a structural unit obtained by dehydration condensation of a hydroxycarboxylic acid in a repeating structure; and a skeleton B which does not include a structural unit obtained by dehydration condensation of a hydroxycarboxylic acid in a repeating structure.Type: GrantFiled: December 27, 2012Date of Patent: May 27, 2014Assignee: Ricoh Company, Ltd.Inventors: Suzuka Amemori, Masahide Yamada, Yoshihiro Moriya, Taichi Nemoto, Yukiko Nakajima, Yoshitaka Yamauchi, Daiki Yamashita, Keiji Makabe, Akiyoshi Sabu
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Patent number: 8735041Abstract: A method for producing a low density resin-coated carrier having a small resin amount to a carrier core material and having a uniform resin coating layer formed on the carrier core material is provided. A resin-coated carrier has a carrier core material and a resin coating layer formed on the surface of the carrier core material. The carrier core material has pores and an apparent density of 1.6 g/cm3 to 2.0 g/cm3 and a remanent magnetization of 10 emu/g of less. The resin coating layer is formed by a dry process of adhering resin particles to a surface of the carrier core material and applying heat and impact force to the resin particles. A volume average particle size of the resin particles is less than 1 ?m. A two-component developer containing the resin-coated carrier is charged in a developing device in an image forming apparatus, and an image is formed.Type: GrantFiled: March 24, 2010Date of Patent: May 27, 2014Assignee: Sharp Kabushiki KaishaInventors: Takanori Kamoto, Takayuki Yamanaka, Osamu Wada, Tadashi Iwamatsu, Nobuyuki Yoshioka
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Patent number: 8735042Abstract: An electrostatic latent image developing toner includes: a release agent; a pigment; and a binder resin. The content of the pigment is from about 10% by weight to about 50% by weight, the glass transition temperature (Tg) of the toner is in the range of from about ?60° C. to about 20° C., and the Young's modulus at 20° C. is in the range of from about 1×100 MPa to about 1×103 MPa.Type: GrantFiled: January 31, 2012Date of Patent: May 27, 2014Assignee: Fuji Xerox Co., Ltd.Inventors: Satoshi Kamiwaki, Kiyohiro Yamanaka, Yusuke Ikeda, Koji Sasaki
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Patent number: 8735043Abstract: Image noise is reduced in halftone imaging processes by placing clear toner particles around the colored toner particles. The clear toner particles must be placed around the colored toner particles prior to transferring the developed image from an imaging member to a receiving member or substrate. The clear toner particles force the colored toner particles to remain in their deposited location during transfer. The result is an image with less mottle and graininess.Type: GrantFiled: April 11, 2008Date of Patent: May 27, 2014Assignee: Xerox CorporationInventor: Chu-heng Liu
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Patent number: 8735044Abstract: A salt represented by the formula (a): wherein Q1 and Q2 each independently represent a fluorine atom etc., X1 represents a single bond etc., X2 represents a single bond etc., Y1 represents a C3-C6 alicyclic hydrocarbon group etc., with the proviso that —X2—Y1 group has one or more fluorine atoms, and Z+ represents an organic counter cation, and a photoresist composition comprising the salt represented by the formula (a) and a resin comprising a structural unit having an acid-labile group and being insoluble or poorly soluble in an aqueous alkali solution but becoming soluble in an aqueous alkali solution by the action of an acid.Type: GrantFiled: May 25, 2010Date of Patent: May 27, 2014Assignee: Sumitomo Chemical Company, LimitedInventors: Koji Ichikawa, Isao Yoshida
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Patent number: 8735045Abstract: A positive resist composition including a base component (A) which exhibits increased solubility in an alkali developing solution under action of acid and an acid-generator component (B) which generates acid upon exposure, the base component (A) including a polymeric compound (A1) containing a structural unit (a0) represented by general formula (a0-1) shown below and a structural unit (a1) derived from an acrylate ester containing an acid dissociable, dissolution inhibiting group.Type: GrantFiled: October 25, 2010Date of Patent: May 27, 2014Assignee: Tokyo Ohka Kogyo Co., Ltd.Inventors: Tomoyuki Hirano, Daiju Shiono, Daichi Takaki
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Patent number: 8735046Abstract: A polymer obtained from copolymerization of a recurring unit having a carboxyl group and/or phenolic hydroxyl group substituted with an acid labile group with a methacrylate having a phenolic hydroxyl-bearing pyridine is useful as a base resin in a positive resist composition. The resist composition comprising the polymer is improved in contrast of alkali dissolution rate before and after exposure, acid diffusion control, resolution, and profile and edge roughness of a pattern after exposure.Type: GrantFiled: November 21, 2011Date of Patent: May 27, 2014Assignee: Shin-Etsu Chemical Co., Ltd.Inventors: Jun Hatakeyama, Koji Hasegawa
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Patent number: 8735047Abstract: A resist composition having a resin having a structural unit represented by the formula (I), a resin being insoluble or poorly soluble in alkali aqueous solution, but becoming soluble in an alkali aqueous solution by the action of an acid and not including the structural unit represented by the formula (I), and an acid generator represented by the formula (II), wherein R1, A1, A13, A14, X12, Q1, Q2, L1, ring W1 and Z+ are defined in the specification.Type: GrantFiled: July 18, 2012Date of Patent: May 27, 2014Assignee: Sumitomo Chemical Company, LimitedInventors: Koji Ichikawa, Satoshi Yamaguchi
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Patent number: 8735048Abstract: An actinic ray-sensitive or radiation-sensitive resin composition comprising (P) a resin having a repeating unit represented by the following formula (1), a resist film using the composition, and a pattern forming method.Type: GrantFiled: January 27, 2011Date of Patent: May 27, 2014Assignee: FUJIFILM CorporationInventors: Takeshi Inasaki, Takayuki Ito, Tomotaka Tsuchimura, Tadateru Yatsuo, Koutarou Takahashi
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Patent number: 8735049Abstract: A method of making a relief printing element in a liquid photopolymer platemaking process is described. The method comprises the steps of: (a) selectively exposing the liquid photopolymer to actinic radiation through a negative to crosslink and cure portions of the liquid photopolymer; and (b) reclaiming uncured portions of the liquid photopolymer to be reused in the platemaking process. The step of reclaiming uncured portions of the liquid photopolymer comprises (i) heating the printing element to decrease the viscosity of the uncured liquid photopolymer; and (ii) removing uncured liquid photopolymer from the surface of the relief image printing element so that recovery of uncured liquid photopolymer from the surface of the relief image printing element is enhanced.Type: GrantFiled: May 22, 2012Date of Patent: May 27, 2014Inventor: Ryan W. Vest
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Patent number: 8735050Abstract: Integrated circuits and methods for fabricating integrated circuits are provided. One method includes creating a master pattern layout including first and second adjacent cells. The first adjacent cell has a first border pin with a first routing line. The second adjacent cell has a second border pin with a second routing line. The first and second routing lines overlap to define an edge-edge stitch to couple the first and second border pins. The master pattern layout is decomposed into sub-patterns.Type: GrantFiled: August 6, 2012Date of Patent: May 27, 2014Assignee: GLOBALFOUNDRIES, Inc.Inventors: Lei Yuan, Hidekazu Yoshida, Jongwook Kye, Qi Xiang, Mahbub Rashed
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Patent number: 8735051Abstract: Exposure apparatus is equipped with an illumination optical device which illuminates a mask with an exposure beam, a mask table which holds a periphery of a pattern area of the mask from above so that a pattern surface of the mask becomes substantially parallel to an XY plane and makes a force at least parallel to an XY plane and on the mask, and a wafer stage which moves along the XY plane, holding a wafer substantially parallel to the XY plane. Therefore, an overlay with high precision of a pattern of a mask and an underlying pattern on the substrate can be realized, even though the exposure apparatus employs a proximity method, that is, the exposure apparatus does not use a projection optical system.Type: GrantFiled: February 28, 2013Date of Patent: May 27, 2014Assignee: Nikon CorporationInventor: Yuichi Shibazaki
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Patent number: 8735052Abstract: A surface modifying material for forming a surface modifying layer provided between a substrate and a resist film, the surface modifying material including an epoxy resin having a weight average molecular weight of 1,000 to 50,000; a method of forming a resist pattern, including: forming a surface modifying layer on a substrate using the surface modifying material, forming a resist film on the substrate, on which the surface modified layer has been formed, using a resist composition, conducting exposure of the resist film, and alkali developing the resist film to form a resist pattern; and a method of forming a pattern, including: etching the substrate, on which a resist pattern has been formed by the method of forming a resist pattern.Type: GrantFiled: March 14, 2011Date of Patent: May 27, 2014Assignee: Tokyo Ohka Kogyo Co., Ltd.Inventors: Isao Hirano, Junichi Tsuchiya
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Patent number: 8735053Abstract: Methods of forming photoresist patterns may include forming a photoresist layer on a substrate, exposing the photoresist layer using an exposure mask, forming a preliminary pattern by developing the exposed photoresist layer and treating a surface of the preliminary pattern using a treatment agent that includes a coating polymer.Type: GrantFiled: May 3, 2011Date of Patent: May 27, 2014Assignee: Samsung Electronics Co., Ltd.Inventors: Subramanya Mayya, Takahiro Yasue, Seok-hwan Oh, Yool Kang
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Patent number: 8735054Abstract: The present disclosure provides a variety of methods and compositions (e.g., solutions) useful for making, sterilizing, and preserving tissues (e.g., acellular tissue matrices). The disclosure also features the acellular tissue matrices made by the methods, which matrices can be used for a variety of applications such as, but not limited to, treating an injury to, or repairing, a large number of tissues and/or organs (e.g., e.g., fascia, bones, and/or cartilage) in a mammal (e.g., a human).Type: GrantFiled: January 2, 2009Date of Patent: May 27, 2014Assignee: LifeCell CorporationInventors: Wendell Sun, Jerome Connor, Qing-Qing Qiu, Rick Owens, Mark Hayzlett
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Patent number: 8735055Abstract: A receptacle having a plurality of interconnected chambers arranged to permit multiple process steps or processes to be performed independently or simultaneously. The receptacles are manufactured to separate liquid from dried reagents and to maintain the stability of the dried reagents. An immiscible liquid, such as an oil, is included to control loading of process materials, facilitate mixing and reconstitution of dried reagents, limit evaporation, control heating of reaction materials, concentrate solid support materials to prevent clogging of fluid connections, provide minimum volumes for fluid transfers, and to prevent process materials from sticking to chamber surfaces. The receptacles can be adapted for use in systems having a processing instrument that includes an actuator system for selectively moving fluid substances between chambers and a detector. The actuator system can be arranged to concentrate an analyte present in a sample.Type: GrantFiled: December 12, 2008Date of Patent: May 27, 2014Assignees: Gen-Probe Incorporated, Qualigen, Inc.Inventors: Scott S. Breidenthal, Sara H. Fan, Richard S. Lee, Norman C. Nelson, Matthew J. Scott, Jason A. Taylor
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Patent number: 8735056Abstract: A new device capable of measuring the number of particles present in a colloidal suspension is disclosed, which includes a forward scatter detector, an extinction detector, a laser beam, a cylindrical lens with which to create a plane of light through which particles can pass, and the various pumps and tubing needed to pass the colloidal suspension through the plane of light. The device is particularly designed for measuring particles which have different refractive indices, and which are in the size range of between about 0.7 to 2 microns. The device can determine the presence or absence of biological particles of interest in a given sample, by incubating a sample with a given ratio of active particles and marker particles, and determining whether the ratio of active particles and marker particles has changed. Additional binding and/or non-binding particles can also be present, and kits including the particles are also disclosed.Type: GrantFiled: May 20, 2010Date of Patent: May 27, 2014Assignee: Invitrox, Inc.Inventors: Paul Toumbas, Don Gabriel
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Patent number: 8735057Abstract: The invention herein disclosed provides for compositions, methods for synthesizing said compositions, and methods for using said compositions, wherein the compositions and methods may be used to bind to and/or deactivate a poison oak oil, such as urushiol. The compositions and methods can be used to treat and/or reduce an inflammatory reaction and/or hypersensitivity to natural compounds found in poison oak, poison ivy, poison sumac, mango, lac tree, and cashew nut.Type: GrantFiled: December 14, 2012Date of Patent: May 27, 2014Assignee: The Regents of the University of CaliforniaInventor: Rebecca Braslau
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Patent number: 8735058Abstract: The present invention relates to certain novel shRNA molecules and methods of use thereof. According to certain embodiments of the present invention, methods for reducing the expression level of a target gene are provided. Such methods generally comprise providing a cell with one or more precursor nucleic acid sequences that encode two or more RNA molecules. A first RNA molecule comprises a double stranded sequence, which includes a guide strand sequence that is complementary to a portion of an mRNA transcript encoded by the target gene. In addition, a second RNA molecule comprises a second double stranded sequence, which includes a second guide strand sequence that is partially complementary to a portion of the mRNA transcript encoded by the target gene. Preferably, the second guide strand sequence comprises one or more bases that are mismatched with a nucleic acid sequence of the mRNA transcript encoded by the target gene.Type: GrantFiled: June 29, 2012Date of Patent: May 27, 2014Assignee: Gradalis, Inc.Inventor: Donald Rao
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Patent number: 8735059Abstract: A method for molecular haplotyping of a subject is disclosed. The method comprises: randomly selecting a set of chromosomes in each of a plurality of lyzed diploid cells of the subject, collecting the selected chromosomes from said plurality of cells into a plurality of sample tubes, wherein each sample tube contains chromosomes selected from one or more cells, genotyping genomic DNA in each sample tube, and determining haplotype of the alleles based on allele nucleotide sequence information and corresponding nucleotide signal intensities from genotyping data. Other methods for molecular haplotyping using single cell lysate or single cell microdissection are also disclosed.Type: GrantFiled: October 6, 2011Date of Patent: May 27, 2014Assignee: Morehouse School of MedicineInventors: Qing Song, Li Ma, Yan Xiao
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Patent number: 8735060Abstract: The invention relates to a purification method for high-purity, DNA-free RNA using a mixture of nanocarrier beads and paramagnetic beads.Type: GrantFiled: July 29, 2010Date of Patent: May 27, 2014Assignee: Stratec Molecular AGInventor: Hans Joos
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Patent number: 8735061Abstract: There is provided a method for determining tumour metastatic potential using biomarkers of tumour metastasis comprising any two of carbonic anhydrase-9 (CAIX), vascular endothelial growth factor C (VEGF-C), ephrin A5 (EFNA5), eph receptor B2 (EPHB2), transforming growth factor beta 3 (TGF-?3), pyruvate dehydrogenase kinase isoenzyme-3 (PDK3), carbonic anhydrase-12 (CAXII), keratin 14 (KRT14), hypoxia inducible factor 1 alpha subunit (HIF-1?), and tenascin C (TNC). CAIX, VEGF-C, EFNA5, EPHB2, TGF-?3 or PDK3 may be indicators of moderate metastatic potential, while CAXII, KRT14, HIF-1?, or TNC may be indicators of high metastatic potential. The biomarkers may be used to assess malignancies or cancers having hypoxic regions, such as breast cancer.Type: GrantFiled: August 10, 2012Date of Patent: May 27, 2014Assignee: British Columbia Cancer Agency BranchInventors: Yuanmei Lou, Paul Christopher McDonald, Arusha Oloumi, Shoukat Dedhar
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Patent number: 8735062Abstract: This invention relates to the detection and quantitation of target nucleic acids in a heterogeneous mixture in a sample and the methods of use thereof. The detection system includes a chemiluminescent molecule, a chemiluminescent substrate, a dye that is light responsive when intercalated into nucleic acids and nucleic acids. This invention is useful in any application where detection of a specific nucleic acid sequence is desirable, or where the detection of enzymes that modify nucleic acids is desirable such as diagnostics, research uses and industrial applications.Type: GrantFiled: December 11, 2012Date of Patent: May 27, 2014Assignee: Beacon Biotechnology LLCInventors: Anthony West, Millard Gambrell Cull
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Patent number: 8735063Abstract: The present invention is directed to methods and reagents for determining the genotype of a hepatitis C virus (HCV) species present in a test sample. The invention more particularly relates to mixtures of degenerate amplification and sequencing primers, and methods of using such primers, that are complementary to a plurality of HCV species, and are capable of generating nucleotide sequence information for a region of NS5B of HCV that is, for each species, indicative of the type and/or subtype, of the species present in the sample.Type: GrantFiled: October 30, 2009Date of Patent: May 27, 2014Assignee: Siemens Healthcare Diagnostics Inc.Inventors: Marcellinus Beld, Remko Gouw, Toumy Guettouche, James Hnatyszyn, Carola van der Meer
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Patent number: 8735064Abstract: The invention relates to the control of gene expression. Specifically, the invention provides compositions and methods for the production and use of recombinant nucleic acid molecules that have the ability to specifically downregulate an expressed target gene in vivo. In some aspects, the invention provides methods for producing a hairpin DNA molecule where part of the molecule is derived from an mRNA that is a target for a small interfering RNA (siRNA) derived from the hairpin. In other aspects, the invention provides synthetic hairpin adapter oligonucleotides that are used in the construction of siRNA-producing cassettes. In other aspects, the invention provides methods for testing for the presence or absence of specific inhibitory activity of an RNAi trigger molecule, and in still other aspects, the invention provides methods for identifying an active RNAi trigger molecule from a library of RNAi trigger molecules.Type: GrantFiled: December 23, 2008Date of Patent: May 27, 2014Assignee: Bergenbio ASInventors: David Micklem, James Lorens
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Patent number: 8735065Abstract: Provided herein are methods and devices for single object detection. The methods and devices can be used to identify a plurality epigenetic markers on a genetic material, or a chromatin, encompassing fragments thereof. The invention provides for the characterization of the genetic material flowing through a channel in a continuous body of fluid based on detection of one or more properties of the genetic material. The methods and systems provided herein allow genome-wide, high-throughput epigenetic analysis and overcome a variety of limitations common to bulk analysis techniques.Type: GrantFiled: February 6, 2012Date of Patent: May 27, 2014Assignee: Cornell UniversityInventors: Harold G. Craighead, Benjamin R. Cipriany, Stephen Levy, Paul Soloway
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Patent number: 8735066Abstract: A specific region of chromosome 10 (10q23.3) has been implicated by series of studies to contain a tumor suppressor gene involved in gliomas, as well as a number of other human cancers. One gene within this region was identified, and the corresponding coding region of the gene represents a novel 47 kD protein. A domain of this product has an exact match to the conserved catalytic domain of protein tyrosine phosphatases, indicating a possible functional role in phosphorylation events. Sequence analyses demonstrated the a number of exons of the gene were deleted in tumor cell lines used to define the 10q23.3 region, leading to the classification of this gene as a tumor suppressor. Further analyses have demonstrated the presence of a number of mutations in the gene in both glioma and prostate carcinoma cells. Methods for diagnosing and treating cancers related to this tumor suppressor, designated as TS10q23.3, also are disclosed.Type: GrantFiled: April 23, 2012Date of Patent: May 27, 2014Assignee: Board of Regents, The University of Texas SystemInventors: Peter Steck, Mark A. Pershouse, Samar A. Jasser, Alfred W. K. Yung, Sean V. Tavtigian
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Patent number: 8735067Abstract: The present invention discloses an asymmetric PCR amplification method, its special primer and application, aims to provide a simple, effective PCR amplification for preparation of single-stranded product. The asymmetric PCR primer of the invention comprises some PCR primer pairs, in which an unrelated nucleic acids sequence to target sequence to be detected is added onto 5?-terminal of one primer. The asymmetric PCR amplification provided includes the steps: 1) preparative denaturing; 2) repetitiously denaturing, primers annealing, extending cycles as the first stage of PCR amplification; 3) repetitiously denaturing, primer extending cycles as the second stage of PCR amplification, wherein an unrelated nucleic acids sequence to target sequence to be detected is added onto 5?-terminal of one PCR primer of each pair in extension.Type: GrantFiled: November 22, 2004Date of Patent: May 27, 2014Assignees: CapitalBio Corporation, Tsinghua UniversityInventors: Zhiwei Zhang, Can Wang, Lingxiang Zhu, Qiong Zhang, Jing Cheng
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Patent number: 8735068Abstract: Long- and/or short-chain nucleic acids are separated, purified and recovered by binding the nucleic acid to a solid phase using a binding buffer, to obtain a bonded nucleic acid, and eluting of the bonded nucleic acid from the solid phase, wherein the binding buffer comprises at least one citric acid salt and at least one alcohol.Type: GrantFiled: May 6, 2010Date of Patent: May 27, 2014Assignee: AJ Innuscreen GmbHInventor: Timo Hillebrand
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Patent number: 8735069Abstract: The invention relates to a method for detecting Kingella microorganisms through PCR of a chaperonin gene.Type: GrantFiled: September 18, 2009Date of Patent: May 27, 2014Assignees: Assistance Publique—Hôpitaux de Paris, Université Paris Diderot-Paris 7Inventors: Stéphane Bonacorsi, Philippe Bidet, Edouard Bingen
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Patent number: 8735071Abstract: The invention features compositions and methods for modulating the expression of Gadd45 and the use of such compositions and methods as neuroprotectants, to enhance neurogenesis, and for the treatment of mood disorders.Type: GrantFiled: December 23, 2009Date of Patent: May 27, 2014Assignee: The Johns Hopkins UniversityInventors: Hongjun Song, Dengke K. Ma, Guo-li Ming
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Patent number: 8735072Abstract: Methods of screening candidate agents to identify lead compounds for the development of therapeutic agents for the treatment of a neurodegenerative disease, such as Huntington's Disease and Parkinson's Disease and methods for identifying a mutation in, or changes in expression of, a gene associated with neurodegenerative disease, such as Huntington's Disease and Parkinson's Disease, are provided.Type: GrantFiled: March 13, 2012Date of Patent: May 27, 2014Assignees: Whitehead Institute for Biomedical Research, University of WashingtonInventors: Paul J. Muchowski, Susan L. Lindquist, Tiago Outeiro
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Patent number: 8735073Abstract: Cannabinoid receptor 2 (CB2) is expressed in B lymphocytes and is involved in immune regulation. Mouse splenic B cells express three CB2 transcripts utilizing two different first exons. Human peripheral blood B cells express one CB2 transcript utilizing one first exon. Alignment of sequenced RACE products to either the mouse or human genome reveals that isolated transcripts contain previously unidentified transcriptional start sites (TSS). B cells from mouse and human preferentially express one transcript, exon 1a in mouse and exon 1 in human. Multiple CB2 TSSs are utilized in mouse splenic B cells and one TSS in human peripheral blood B cells. The defining of the receptor gene TSSs in these cells provides materials and methods for therapeutically regulating immune function, including antibody isotype switching, using compounds such as inhibitory nucleic acids that down-regulate expression of the B cell CB2 gene (e.g., RNAi molecules).Type: GrantFiled: September 23, 2013Date of Patent: May 27, 2014Assignee: University of South FloridaInventors: Thomas W. Klein, Tracy Sherwood, Liang Nong, Cathy Newton
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Patent number: 8735074Abstract: This application describes miRNAs that may be used as serum or plasma biomarkers for characterizing lung disease in a patient. These miRNA biomarkers may be used alone or in combination with other markers for the diagnosis, prognosis, or monitoring of diseases such as lung cancer.Type: GrantFiled: August 27, 2010Date of Patent: May 27, 2014Assignee: Asuragen, Inc.Inventors: Elizabeth Mambo, David Brown, Alex T. Adai, Tiffany Sanford, Stephanie Volz, Ashish Choudhary
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Patent number: 8735075Abstract: The present invention relates to detection of cancer, or assessment of risk of development thereof. In particular, the present invention provides compositions and methods detection of field carcinogenesis by identification of ultrastructural and molecular markers in a subject.Type: GrantFiled: February 17, 2012Date of Patent: May 27, 2014Assignees: Northwestern University, Northshore University HealthsystemInventors: Vadim Backman, Hariharan Subramanian, Dhwanil Damania, Hemant Roy, Dhananjay Kunte, Mart De La Cruz
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Patent number: 8735076Abstract: Provided herein are targets that can be used for the diagnosis, prognosis and therapy of a variety of cancers.Type: GrantFiled: March 12, 2012Date of Patent: May 27, 2014Assignee: The Cleveland Clinic FoundationInventors: Charis Eng, Frank Weber
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Patent number: 8735077Abstract: Provided is a field effect transistor (FET) type biosensor including a source electrode, a gate, and a drain electrode. A ligand that can bind to a side of a nucleic acid is added to the surface of the gate. In a conventional FET type biosensor, it is difficult to detect a signal within the debye length because a target nucleic acid is directly fixed to the surface of a gate of the conventional FET. However, in the present invention, this problem can be overcome and the debye length can be increased by treating the surface of a gate of an FET sensor with a ligand that can bind to a side of a nucleic acid. The ligand can be adsorbed onto the surface of the gate. In this case, the nucleic acid is adsorbed parallel to the surface of the gate, not perpendicular to the surface of the gate, thus generating an effective depletion region. In addition, hybridization efficiency can be increased because a hybridized sample can be injected into an FET sensor at high ionic strength.Type: GrantFiled: January 20, 2006Date of Patent: May 27, 2014Assignee: Samsung Electronics Co., Ltd.Inventors: Jeo-young Shim, Su-hyeon Kim, Kyu-tae Yoo, Sung-ouk Jung, Joon-shik Park
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Patent number: 8735078Abstract: The present invention relates to a method for the in vitro diagnosis of colorectal cancer by determining the presence of the Apolipoprotein AII tumor marker in a biological sample taken from a patient suspected of having colorectal cancer. Said method can be used for early diagnosis, screening, therapeutic follow-up and prognosis, and also for relapse diagnosis in relation to colorectal cancer.Type: GrantFiled: July 10, 2008Date of Patent: May 27, 2014Assignee: BiomerieuxInventors: Yasemin Ataman-Onal, Jean-Philippe Charrier, Genevieve Choquet-Kastylevsky, Florence Poirier
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Patent number: 8735079Abstract: An in vitro method for the risk stratification of patients with stable arteriosclerosis, especially stable coronary artery disease, is disclosed wherein the concentration of procalcitonin is determined in the circulation of such patients using a highly sensitive PCT assay, and wherein within the range of PCT concentrations in the typical normal range of healthy individuals cutoff values are defined which distinguish groups of individual patients with stable arteriosclerosis in accordance with personal cardiac risk, and patients are allotted to one of said risk groups on the basis of their individual PCT concentrations.Type: GrantFiled: August 1, 2008Date of Patent: May 27, 2014Assignee: B.R.A.H.M.S GmbHInventors: Andreas Bergmann, Joachim Struck, Nils G. Morgenthaler, Jana Papassotiriou, Stefan Blankenberg, Karl Lackner, Hans Rupprecht, Christoph Bickel
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Patent number: 8735080Abstract: Methods and devices for diagnosing, monitoring, or determining obstructive uropathy or an associated disorder in a mammal are described. In particular, methods and devices for diagnosing, monitoring, or determining obstructive uropathy or an associated disorder using measured concentrations of a combination of three or more analytes in a test sample taken from the mammal are described.Type: GrantFiled: August 6, 2010Date of Patent: May 27, 2014Assignee: Rules-Based Medicine, Inc.Inventors: Samuel T. Labrie, James P. Mapes, Ralph L. McDade, Dominic Eisinger, Karri L. Ballard, Michael D. Spain