Abstract: An object of the present invention is to provide a lubricant composition for a limited slip differential comprising a major amount of an oil of lubricating viscosity and an oil soluble compound, as well as a method of using the same in a differential. The compound can be of formula R?NH(CH2)3NHCOR?, wherein R? can be a C8-28 amine, and —COR? can be derived from a C8-28 acid.

Abstract: The present invention relates to a lubricant composition for a marine engine, in particular for a four-stroke or two-stroke marine engine, comprising at least one base oil, at least one alkoxylated fatty amine, at least one hydrogenated styrene-isoprene copolymer and at least one detergent.

Abstract: Liquid diphenylamine compositions comprising predominately p,p?-di-alkylated diphenylamines and low amounts of mono-alkylated diphenylamines are prepared by adding an olefin to a mixture comprising mono-alkylated diphenylamines, di-alkylated diphenylamines, Lewis acid catalyst and an amount of another olefin sufficient to suppress dealkylation of the alkylated diphenylamines.

Abstract: A gelling nanofluid and methods for manufacture are provided. The composition and methods for manufacture produce nanofluid gels so that the settlement of nanoparticles in a base fluid is improved due to the inhibition of particle movement in the gel. The nanofluid gel is produced by using a gelling agent which is either coated on the nanoparticles prior to dispersion in the base fluid or directly introduced in the base fluid.

Abstract: The present invention is directed towards an aqueous lubricant composition comprising hyaluronic acid, organic acid, salts and water, and has a pH of between 5.5 and 7.0 and an osmolality between about 150 and 1,500 mOsm/kg. The lubricant surprisingly has long-lasting lubricity and low osmolality as compared to conventional lubricants on the market.

Abstract: An oleaginous corrosion-resistant lubricating composition comprising at least one alpha-olefin synthetic oil and effective amounts of metal salts of sulfonic acids/carboxylic acids, alkylated aromatic compounds as solubility modifiers, pour-point depressants, at least one antioxidant, and metal deactivators.

Abstract: Biological compositions containing animal fats and plant oils desirably are free of contaminants prior to processing into a biofuel. Disclosed herein is a method of removing such contaminants from these compositions to make that processing more efficient. The method employs a unique arrangement of mixers and centrifuges along with acidic solutions and recycle streams to re move these contaminants from the compositions.

Abstract: The present invention provides a fatty acid mixture enriched with palmitoleic acid and palmitoleic acid derivatives relative to the starting lipid mixture; fractionations procedure describe herein are winterization methods. The invention provides methods of dry winterization and solvent-aided winterization alone and in combination. In some embodiments, the palmitoleic acid and palmitoleic acid derivative enriched mixture described herein contains less than 1% of palmitic acid and palmitic acid derivative. The invention also provides palmitoleic derivatives of use as dietary supplements.

Abstract: The present invention pertains to a method of counteracting malodor by introducing a malodor counteracting effective amount of novel cyclohexanol compounds, wherein the compounds are represented by the following formula: an isomer or a mixture of isomers thereof, wherein one of R and R? represents hydrogen with the other representing a C1-C6 linear, branched or cyclic alkyl, alkenyl, alkynyl or aromatic group; and one of the dashed lines represents a carbon-carbon single bond with the other representing a carbon-carbon double bond.

Abstract: The invention concerns an isononyl ester mixture of an epoxidized fatty acid mixture, the fatty acid mixture having been obtained from a vegetable oil, the fraction of saturated fatty acids in the isononyl ester mixture being below the fraction of saturated fatty acids in the vegetable oil from which the fatty acids have been obtained.

Abstract: The present invention relates to automatic dishwashing detergent compositions comprising a) one or more compounds of the formula (I) RO—(CH2CH2O—)nCH2—COOM??(I), wherein R is a linear or branched saturated alkyl group comprising from 8 to 30 carbon atoms or a linear or branched unsaturated alkenyl group with one or more double bonds and comprising from 8 to 30 carbon atoms, n is a number from 1 to 20, and M is a counter ion, b) one or more builder substances, and c) one or more bleaching agents, wherein the automatic dishwashing detergent compositions contain no chloride ions or wherein the total amount of chloride ions in the automatic dishwashing detergent compositions is below 0.5% by weight, referring to the total composition, and which are characterized in that the automatic dishwashing detergent compositions are free of nonionic surfactants.

Abstract: A fabric conditioner composition comprising: a cationic fabric softener, and 0.02 to 0.32% by weight of an amino-functional, epoxide group containing silicone polymer having a weight average molecular weight of 400,000 to 900,000. Also, a method of reducing wrinkles on fabric during laundering comprising laundering the fabric with a composition comprising 0.02 to 0.32% by weight of an amino-functional, epoxide group containing silicone polymer having a weight average molecular weight of 400,000 to 900,000. The polymer is unexpectedly effective at low levels of use.

Abstract: The present invention provides synergistic biocidal composition such as a synergistic detergent composition for use, e.g.

Abstract: Embodiments of the invention generally provide methods for cleaning a UV processing chamber component. In one embodiment, a method for cleaning a UV processing chamber component includes soaking the chamber component having a SiCO residue formed thereon in a cleaning solution for about 1 to 10 minutes. The cleaning solution comprises about 5% by weight to about 60% weight of NH4F and about 0.5% by weight to about 10% by weight of HF. The method also includes polishing the chamber component. In another embodiment, a method of cleaning a processing chamber component fabricated from quartz includes soaking the chamber component having a SiCO residue formed thereon in a cleaning solution comprising about 36% by weight of NH4F and about by weight of HF for about 3 minutes. The method also includes applying an ultrasonic power to the cleaning solution, and mechanically polishing the chamber component.

Abstract: Cleaning products comprising flexible water-soluble articles and flexible water-insoluble films.

Abstract: An alcoholic beverage is aged in at least one wooden barrel after the barrel is covered by a film having an oxygen transmission rate of at least 0.5 cc/m2/day and an ethanol transmission rate of less than 30 g/m2/day. The beverage acquires one or more specific flavor components in an amount of 50%, 75%, 90%, or 100% relative to amounts of one or more of the same flavor components in a control alcoholic beverage aged in at least one wooden barrel without the film thereon. Covering the barrel with the film reduces the angels' share evaporative loss, while allowing the beverage to develop desirable flavor components during aging. In another embodiment, the process reduces angels' share evaporative loss while achieving organoleptics indistinguishable from a control.

Abstract: A photobioreactor (100) for use in treating polluted air and producing biomass may comprise, at least in part, a generally vertical tube or fluidic pathway (102), a generally vertical helical tube or fluidic pathway (104) having a light source (106) partially positioned within the helical fluidic pathway (104), a head cap assembly (108), and a base assembly (110). In one illustrative example, the light source (106) may be a light emitting diode (LED) or a plurality of light emitting diodes (LEDs). By one approach, a gas diffusion apparatus (112) is located at the base assembly (110) adjacent the generally vertical fluidic pathway (102).

Abstract: A tray (100; 400; 500; 600) for accommodating a cell culture (101), such as an embryo, for use during culturing thereof and for optical monitoring of the cell culture, e.g. during in vitro fertilization comprises a carrier structure (104;404;504;604) defining at least one accommodating zone (102; 402; 502; 602) for accommodating the cell culture. At least one focal lens (110; 410; 510; 610), notably a numerical aperture increasing lens is integrally formed with the carrier structure to facilitate monitoring of the cell culture through the carrier structure. A diameter of the focal lens may exceed a diameter of the at least one accommodating zone. The focal lens may be integrally moulded with the carrier structure from a thermoplastic material.

Abstract: The present invention relates to the devices and method comprising microelectrode arrays for the differentiation, maturation and functional analysis of electroconductive cells, including muscle cells (including, but not limited to, cardiomyocytes, skeletal muscle myocytes and smooth muscle myocytes) and neuronal cells. The microelectrode present on the arrays can be used to stimulate and record from cells cultured on the substrate. In some embodiments, the substrate has a substantially smooth surface, and in other embodiments the substrate is nanotextured, including an array of substantially parallel grooves and ridges of nanometer-micrometer widths.

Abstract: The present invention relates to a measurement probe system (6) comprising a reusable unit, a pluggable electrical connector (12) and a disposable, single-use sensing unit (14), the reusable unit comprising a connector support (27), the disposable, single-use sensing unit (14) comprising a sealing sleeve (32) and a sensing device (36). The sensing device (36) is configured to be fixedly connected to the sealing sleeve (32). The pluggable electrical connector (12) comprises a connecting portion (28) configured to be connected to the connector support (27) and a complementary connecting portion (30), which is connected to the sensing device (36) and to the sealing sleeve (32).

Abstract: The present invention relates to the field of testing and evaluation of biological samples and provides apparatus for cultured samples comprising at least one independently accessible module adapted for incubating at least one of a plurality of samples wherein the at least one module is operatively associated with a light source and a movable optical inspection means adapted for motion about a viewing axis through the module to enable a sweeping of viewing area. The invention also provides a method of assessing cultured samples for viability comprising the steps of: disposing biological samples in a substantially elliptical arrangement within a culturing chamber of an independently accessible module; imaging individual samples of the substantially elliptical arrangement with optical inspection means driven within an X-Y plane that is normal to a viewing axis through the module to obtain time lapse measurement of development of individual samples.

Abstract: In culture vessels for producing biological samples, it is possible to prevent decrease in a temperature at the time of taking out the culture vessels from an automatic culture apparatus and during delivery of the culture vessels which have been taken out from the automatic culture apparatus. This invention includes a culture vessel 201 holding a biological sample therein, a heat storage portion 701 holding the culture vessel, and a heat insulation portion 601 surrounding the heat storage portion. The whole circumference or a part of 609, 610, and 611 of the heat insulation portion 601 is removable depending on an arrangement environment.

Abstract: This invention relates to devices and methods for purifying, detecting and using biological cells. A variety of cell types including viable tumor, stem, immune and sperm cells can be purified from a complex biological sample using a column, including a pipette tip column. Methods of the invention can aid research, diagnosis and treatment of cancer. Purified viable cells can be detected on the column or eluted from the column and detected. Cells on a column can be used as a stationary phase for liquid chromatography. Cells may be removed, recovered and analyzed.

Abstract: A sperm selection unit structure for efficiently separating sperm having favorable motility includes: a storage part which stores semen acquired from an animal therein; a buffer solution flow passage which communicates with the storage part and in which a buffer solution flows in the direction toward the storage part as a laminar flow; and a swim-up passage which communicates with the storage part such that the sperm swim up in the buffer solution flow passage from the storage part, wherein a downstream part of the swim-up passage is formed of a flow passage having a wide width where the buffer solution flows as a slow flow while maintaining a laminar flow state of the buffer solution, and a downstream end portion of the flow passage is opened in a state where the downstream end portion faces a wall surface of the storage part.

Abstract: A device (1) and a method are provided for treating a porous filtration medium (37) having a receiving unit (2) with of a receiving part (5) and a base part (6). The porous filtration medium (37) can be lifted by the receiving part (5) from a lower part (33) of a filtration device (32), and the receiving part (5) with the porous filtration medium (37) can be mounted on the base part (6). The receiving part (5) is latchable to the base part (6). The base part (6), towards the filtration medium (37) has an incubation chamber (17) connected to a base part (6) outlet (3) that faces away from the receiving part (5), and the outlet (3) has a projection onto which a receiving vessel (4) containing a solvent (28) for dissolving the porous filtration medium (37) can be detachably pushed on.

Abstract: Strains of Toxoplasma gondii isolated from their natural environment for their use in the prevention and/or the treatment, in a mammal, of ocular lesions associated with an infection by an apicomplexan of the Sarcocystidae family.

Abstract: The invention provides a novel bacterium with improved properties, including improved ethanol production, ethanol productivity, CO uptake, specific growth rate, ethanol to acetate ratio, and alcohol tolerance. The bacterium may be derived from Clostridium autoethanogenum and/or may comprise at least one DNA or amino acid sequence selected from SEQ ID NOs: 1, 3, 6, 8, 10, 12, 14, and 16. In one embodiment, the bacterium is Clostridium autoethanogenum deposited under DSMZ accession number DSM23693 or a bacterium derived therefrom.

Abstract: A serum free cell culture media, wherein the media is adapted to be conditioned by culturing a first set of eukaryotic cells in the media, wherein the first set of eukaryotic cells use an expression vector to excrete levels of desired complex proteins into the media; wherein said desired complex proteins include human Growth Hormone (hGH), Growth Hormone-like growth factors, insulin-like growth factors, insulin, modified insulins, cytokines, mitogenic proteases and mixtures thereof; and wherein the media is adapted to grow a set of eukaryotic cells.

Abstract: A protein nanofiber comprised of self-assembling pentamers, and a method for producing the protein nanofiber, in which the protein upon which the nanofiber is based is a specific form of COMP. The proteins self-assemble via electrostatic interactions to form fibers that extend longitudinally.

Abstract: The present invention relates to technology of immobilizing or coating various functional bioactive substances on various surfaces without physical chemical treatment using mussel adhesive protein. More specifically, the present invention relates to a functional scaffold for tissue engineering comprising artificial extracellular matrix, manufactured by coating various functional bioactive substances on the surface of nanofiber and metal scaffold using mussel adhesive protein, and a method of manufacturing the same.

Abstract: Provided herein are methods of culturing a mammalian cell and various methods that utilize these culturing methods. Also provided are multi-well cell culture plates, e.g., for use in perfusion culturing methods.

Abstract: An expression system for expressing a protein comprising: a eukaryotic host cell carrying a dihydrofolate reductase (DHFR) deficiency; and an expression vector, the expression vector encoding the human growth hormone gene; a expression vector, the expression vector comprising: a eukaryotic selectable marker including a minimal SV 40 early promoter driving expression of a sequence encoding dihydrofolate reductase for complementing the DHFR deficiency in the host cell; a prokaryotic selectable marker conveying Ampicillin resistance to a prokaryotic host cell; a prokaryotic Origin of Replication; a plurality of multiple cloning sites (MCS); and at least one protein expression module comprising: a Simian Vacuolating Virus 40 (SV40) early promoter, inclusive of its 72 bp enhancer repeats; and a rabbit ?-globin intron sequence being separable from a SV40 p A sequence by a first multiple cloning site, for receiving a coding sequence and expressing a desired protein therefrom.

Abstract: Provided herein are placental stem cells that exhibit increased survival (“enhanced placental stem cells”), compositions comprising such placental stem cells, and methods of using such placental stem cells and compositions.

Abstract: The present invention features assays for co-culturing primary cells while maintaining key biological activities specific to the primary cells. The invention is based, at least in part, on the discovery that compositions and methods for primary cells in a high-throughput co-culture platform, image analysis for distinguishing cells in co-cultures and assays that are suitable for screening of agents in epithelial cells, such as hepatocytes.

Abstract: The present invention discloses a cyprinid herpesvirus II-sensitive brain tissue cell line of Carassius auratus gibelio and establishing method and use thereof. The cell line is deposited in China Center for Type Culture Collection under an accession number of CCTCC No: C2013179. The brain tissue cell line of Carassius auratus gibelio is in good growth state and sensitive to CyHV-2 that is presently hardly cultured with ordinary fish cell lines. After six passages of CyHV-2 in GiCB cells, viral nucleic acid can still be detected and a cytopathic effect is stable. When ultrathin microscopic sections are prepared from cells having the cytopathic effect, considerable mature CyHV-2 virions and their replication process can be observed in GiCB cells. The construction method of the brain tissue cell line of Carassius auratus gibelio of the present invention has high repeatability, scientific and reasonable conditions.

Abstract: Differentiation and stability of neural stem cells can be enhanced by in vitro or in vivo culturing with one or more extracellular matrix (ECM) compositions, such as collagen I, IV, laminin and/or a heparan sulfate proteoglycan. In one aspect of the invention, adult mammalian enteric neuronal progenitor cells can be induced to differentiate on various substrates derived from components or combinations of neural ECM compositions. Collagen I and IV supported neuronal differentiation and extensive glial differentiation individually and in combination. Addition of laminin or heparan sulfate to collagen substrates unexpectedly improved neuronal differentiation, increasing neuron number, branching of neuronal processes, and initiation of neuronal network formation. In another aspect, neuronal subtype differentiation was affected by varying ECM compositions in hydrogels overlaid on intestinal smooth muscle sheets.

Abstract: The present invention provides compositions and methods for inhibiting Ikaros in a cell in order to enhance the cytolytic activity of the cell. In one embodiment, the cells may be used in adoptive T cell transfer. For example, in some embodiments, the cell is modified to express a chimeric antigen receptor (CAR). Inhibition of Ikaros in T cells used in adoptive T cell transfer increases cytolytic activity of the T cells and thus may be used in the treatment of a variety of conditions, including cancer, infection, and immune disorders.

Abstract: A system for extraction of cells from a sample of tissue comprises a main device (1) and a disposable kit (4) for use with said main device; said main device (1) is configured to host at least one disposable kit and to execute at least one preset program; said disposable kit (4) comprises a work chamber (11) for the sample of tissue and a plurality of solution bags (12), the disposable kit comprises also fluid transfer means (14, 19) operating between the work chamber and the solution bags (12) and controlled by the main device via suitable interface means; in a preferred embodiment the disposable kit (4) is mounted on a drum and contained in an active capsule carried by a rotary carousel; claims are also directed to the disposable kit for use in said system.

Abstract: Insulin producing ? cells are found in three dimensional (3D) structures, the Islet of Langerhans. The 3D structure is required for normal ? cell function and survival. ? cell pseudoislets (PIs) are useful for study of ? cell physiology. Co-culturing of primary human islets and ? cell lines together with islet-derived epithelial cells can improve ? cell function and survival and maintain the cells' 3D structure, resulting a rapid and spontaneous formation of free-floating PIs. ? cells in PIs were similar in size to native islets and showed increased percentage of pro-insulin-positive cells, increased insulin gene expression in response to glucose stimulation, improved glucose-stimulated insulin secretion, and reduced ? cell death. Key ECM proteins, absent in monolayer ? cells, are deposited by iECs in and round the PIs. iEC induced PIs are a useful tool for examining ?-cell/iEC interactions and studying ?-cell function in a native 3D configuration.

Abstract: Disclosed are genetically modified human pancreatic progenitor cells, comprising an exogenous nucleic acid molecule encoding TGIF2, for use as a medicament in the treatment of a subject with diabetes, in addition to methods for the production of said cells. Also disclosed is an expression vector encoding TGIF2 for use as a medicament in the treatment of a subject with diabetes.

Abstract: A method of ex-vivo increasing insulin content in progenitor cells which express Zinc Finger E-Box Binding Homeobox 1 (ZEB-1) is disclosed. The method comprises contacting the progenitor cells with an inhibitory agent directed against a polypeptide, wherein the RNA transcript encoding said polypeptide is targeted by miRNA-200c, said polypeptide being selected from the group consisting of ZEB-1, SOX-2 and SOX-6.

Abstract: Provided herein are methods of culturing a mammalian cell and various methods that utilize these culturing methods.

Abstract: Aspects of the invention relate to methods and related compositions for preferentially targeting cancer stem cells. In some embodiments, the methods utilize PKC-?/FRA1 pathway inhibitors to target carcinoma cells. Also provided are methods for identifying a candidate compound for selectively inhibiting growth of cancer stem cell, and methods for obtaining cells that have undergone an epithelial to mesenchymal transition.

Abstract: The disclosure provides cancer stem cells, for use in stimulating immune response against a cancer, such as hepatocellular carcinoma (HCC). Methods for preparing and purifying the cancer stem cells are provided.

Abstract: Modified E1a regulatory sequences are provided, wherein at least one Pea3 binding site, or a functional portion thereof, is deleted. Also provided are modified E1a sequences that selectively express particular isoforms. Also provided is an E1b-19K clone insertion site. These modified sequences can be used individually, or in combination with one another, to provide tumor-selective expression of proteins.

Abstract: The present disclosure provides infectious recombinant adeno-associated virus (rAAV) virions that comprise a variant capsid protein and a heterologous nucleic acid. The present disclosure further provides the variant adeno-associated virus (AAV) capsid proteins (and/or a nucleic acid encoding the variant AAV capsid proteins), which confer to an infectious rAAV virion an increased resistance to human AAV neutralizing antibodies. The present disclosure further provides host cells comprising an infectious rAAV virion and/or a nucleic acid encoding a subject variant AAV capsid protein. The present disclosure further provides methods of delivering a heterologous nucleic acid to a target cell where the target cell is contacted with a subject infectious rAAV virion. The present disclosure further provides methods of delivering a gene product to an individual, the methods generally involving administering an effective amount of a subject rAAV virion to an individual in need thereof.

Abstract: The present invention relates to 3-hydroxypropionate dehydrogenase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.

Abstract: Disclosed herein is a recombinant Bacillus manganese oxidase complex, including a bacterial expression vector for the expression of the complex and a method of synthesizing the complex.

Abstract: The present invention relates to mutants of Cre recombinase.

Abstract: Disclosed are methods of making and using engineered Fold cleavage domain variants. Also disclosed are methods, compositions and fusion proteins containing obligate heterodimers of engineered Fold cleavage domain variants and DNA binding domains, such as zinc finger protein (ZFP) domains and transcription activator-like effector (TALE) domains.