Abstract: The invention provides for LNA oligomers, for the treatment of a metabolic or liver disorder, wherein the LNA oligomer is administered orally in a unit dose of less than 50 mgs/kg, wherein the LNA oligomer is administered in the presence of a penetration (permeation) enhancer.

Abstract: The present description relates to an inhibitory RNA molecule, comprising an oligonucleotide that selectively knocks down expression a Nanog pseudogene expressed in many human cancers, a replicating viral vector capable of encoding such inhibitory RNA molecule, pharmaceutical compositions comprising said vector, and methods of treating cancer by administration of said pharmaceutical composition.

Abstract: Nucleic acid aptamers which bind specifically to or are internalised into cells expressing a CD7 cell-surface receptor are disclosed. The aptamers comprise a nucleic acid molecule having a sequence which is at least 80% identical to 5?-GG-GAGACAAGAAUAAGCAUG-R1-UUCGACAGGAGGCUCACAACAGnynz-3? (SEQ ID NO: 3). The aptamers can be linked to therapeutic or diagnostic molecules, and can be used to deliver the therapeutic or diagnostic molecules to cells expressing the CD7 receptor. The aptamers can also be used as detection tools.

Abstract: Compositions and methods for modifying genetic material are provided. One embodiment provides aptamers capable of binding to a site-specific DNA binding moiety to facilitate the exchange of homologous genetic information between a donor molecule and the desired target locus (aptamer-guided gene targeting or AGT). One embodiment provides an oligonucleotide containing a aptamer, preferably a DNA aptamer at the 5? end. The oligonucleotide also contains a region of homology, also referred to as donor DNA, to a desired nucleic acid, locus, or gene. The DNA binding moiety can be a nucleic acid, a protein, or a complex of proteins. In a preferred embodiment the DNA binding moiety is a homing endonuclease that cuts DNA to facilitate the modification of the DNA by the donor DNA.

Abstract: The present invention relates to an aptamer or an active fragment thereof raised against the semi-conserved duffy binding ligand domain 1?, DBL1?, region of the Plasmodium falciparum erythrocyte membrane protein 1, PfEMPI, which aptamer has an effect against malaria, in particular severe cerebral malaria.

Abstract: One embodiment of the invention provides a genetically enhanced Chlorogloeopsis sp. host cell comprising at least one first recombinant gene encoding a first protein for the production of ethanol under the transcriptional control of a first inducible promoter, having at least 85%, 90% or 95% sequence identity to an endogenous inducible promoter of the Chlorogloeopsis sp. host cell.

Abstract: Methods and kits for joining three or more polynucleotides to form a product polynucleotide are provided. Such a method includes forming a reaction mixture comprising (i) a vector fragment whose ends have four base overhangs resulting from cleavage of a vector with a first type IIs enzyme, (ii) a first insert nucleic acid with a four base overhang at one end resulting from cleavage by the first type IIs enzyme and a three base overhang at the other end resulting from cleavage by the second type IIs enzyme; and (iii) a second insert nucleic acid with a four base overhang at one end resulting from cleavage by the first type IIs enzyme and a three base overhang at the other end resulting from cleavage by the second type IIs enzyme, and (iv) a ligase.

Abstract: The present invention relates to the application of isolated promoters and synthetic constructs for efficient production of genetically modified cells in a species selected from the Pucciniomycotina and Ustilaginomycotina subphyla, in particular, species selected from the Rhodosporidium, Rhodotourla, Sporobolomyces or Pseudozyma genus.

Abstract: Methods for the identification of variant recognition sites for rare cutting engineered double strand break inducing agents and compositions thereof are provided. Further provided are nucleic acid constructs, yeast, plants, plant cells, explants, seeds and grain having the of variant recognition sites. Various methods of identifying variant recognition sites with increased substrate activity for a rare cutting engineered double strand break inducing agents are provided.

Abstract: MPO1 and MPO2 can be regulated for either decreasing or increasing alkaloid levels in plants, in particular in Nicotiana plants. In particular, suppressing or overexpressing one or more of MPO1 and MPO2 may be used to decrease or increase nicotine and nicotinic alkaloid levels in tobacco plants. Suppression or overexpression of one or more of MPO1 and MPO2 may be used in combination with modification of expression of other genes encoding enzymes on the nicotinic alkaloid biosynthetic pathway such as A622, NBB1, PMT, and QPT.

Abstract: MPO1 and MPO2 can be regulated for either decreasing or increasing alkaloid levels in plants, in particular in Nicotiana plants. In particular, suppressing or overexpressing one or more of MPO1 and MPO2 may be used to decrease or increase nicotine and nicotinic alkaloid levels in tobacco plants. Suppression or overexpression of one or more of MPO1 and MPO2 may be used in combination with modification of expression of other genes encoding enzymes on the nicotinic alkaloid biosynthetic pathway such as A622, NBB1, PMT, and QPT.

Abstract: This invention provides methods for producing a non-natural hybrid seed. Also disclosed are specific miRNAs and miRNA recognition sites useful for conferring inducible sterility on a crop plant, and recombinant DNA construct including such exogenous miRNA recognition sites.

Abstract: The invention is directed to artificial transcription regulating polynucleotides that confer guard cell regulated expression and methods of use thereof. The present invention further provides methods of using the transcription regulating polynucleotides and plants and plant parts thereof comprising the transcription regulating polynucleotides. In agricultural biotechnology, plants can be modified according to one's needs. One way to accomplish this is by using modern genetic engineering techniques. For example, by introducing a gene of interest into a plant, the plant can be specifically modified to express a desirable phenotypic trait.

Abstract: The present invention relates to a novel gene, IbENOD93, and transgenic plants using the same. More specifically, the present invention provides an IbENOD93 gene, an open reading frame (ORF) of the IbENOD93 gene, a recombinant vector comprising the gene or the ORF, and a transformant transformed with the vector. Moreover, the present invention provides a composition for enhancing root thickening growth and promoting maturation in a plant having storage root(s). Furthermore, the present invention provides a method for producing a transgenic plant having storage root(s) with enhanced thickening growth, and a method for regulating or enhancing root thickening growth and maturation in a plant having storage root(s). According to the present invention, it is possible to promote the thickening growth of storage roots as well as the growth of aerial part.

Abstract: Methods for producing pathogen-inducible promoters for the expression of genes in plants are provided. The pathogen-inducible promoters are inducible by one, two, three, or more plant pathogens. Methods for producing R genes that are inducible in a plant by more than one plant pathogen are further provided.

Abstract: Methods and compositions for modulating Slm1 are provided. Methods are provided for modulating the expression of Slm1 in a host plant or plant cell to modulate agronomic characteristics.

Abstract: This disclosure relates to the isolation and sequencing of nucleic acid molecules that encode cytochrome P450 polypeptides from a Papaver somniferum cultivar; uses in the production of noscapine and identification of poppy cultivars that include genes that comprise said nucleic acid molecules.

Abstract: The present invention provides the identification and use of EGTom1 and/or EGTom2, homologs of EGTom1 and/or EGTom2, orthologs of EGTom1 and/or EGTom2, paralogs of EGTom1 and/or EGTom2, and fragments and variations thereof for altering salt tolerance, drought tolerance and/or sugar content of fruit (sweetness) in plants. The invention relates to the identification and use of nucleic acid sequences for salt/drought tolerance and fruit sweetness in plants.

Abstract: The present disclosure provides methods and compositions for modifying fatty acids in Camelina sativa oil. Fatty Acid Desaturase 2 (FAD2), Fatty Acid Desaturase 3 (FADS), and/or Fatty Acid Elongase 1 (FAE1) genes regulate fatty acid composition in camelina oil.

Abstract: The present invention is directed to an improved poppy straw, concentrate of poppy straw and opium of Papaver somniferum for the production of thebaine containing little or no oripavine, codeine or morphine. The present invention also provides plants, stands and seeds of Papaver somniferum and methods for the production of thebaine.

Abstract: An object of the present invention is to provide nucleic acids capable of imparting high-yielding ability to plants. Another object of the present invention is to use such nucleic acids to produce transgenic plants at increased yield, as well as to provide methods for increasing the yield of plants. By introducing into a plant a construct in which a promoter of a pseudo-response regulator gene in O. longistaminata and/or a structural gene of a pseudo-response regulator in a plant are operably linked, a transgenic plant is obtained that has acquired high-yielding ability.

Abstract: The present invention provides methods for obtaining plants that exhibit useful traits by expression of a recombinant DNA methyltransferase in progenitor plants. Methods for identifying genetic loci that provide for useful traits in plants and plants produced with those loci are also provided. In addition, plants that exhibit the useful traits, parts of the plants including seeds, and products of the plants are provided as well as methods of using the plants. Recombinant DNA vectors and transgenic plants comprising those vectors that express a recombinant DNA methyltransferase are also provided.

Abstract: The present disclosure embraces Kalanchoë interspecific hybrid plants, and considers rol transformation in Kalanchoë species and hybrids. Disclosed herein are methodology and the like for producing rol-transformed Kalanchoë interspecific hybrid plants, as well as resultant rol-transformed Kalanchoë interspecific hybrid plants with novel phenotypes.

Abstract: The present invention pertains to a method for increasing the photosynthesis and yield/growth of plants, and a transgenic plant which is used in the method.

Abstract: Methods of increasing the resistance of a crop plant to heat stress and in particular methods of improving the grain yield and quality of crop plants grown under heat stress in the form of increased minimal temperatures are provided. The methods include selecting plants with increased expression of HYR and growing these plants in regions expected to experience minimal temperatures above 25° C. during the growing season. Methods of screening plants for increased resistance to heat stress and methods of producing grain in regions having minimal temperatures of 25° C. or more are also provided.

Abstract: The present invention relates to pathogen-resistant plants. In one aspect, plants comprising a heterologous expression cassette are provided, wherein the expression cassette comprises a polynucleotide that inhibits expression of a fungal pathogen dicer-like (DCL) gene and wherein the plant has increased resistance to a fungal pathogen compared to a control plant lacking the expression cassette. Methods of making and cultivating pathogen-resistant plants are also provided.

Abstract: A transgenic soybean resistant to soybean cyst nematode (SCN), or parts thereof, including an artificial DNA construct encoding a serine hydroxymethyltransferase protein (e.g., GmSHMT). Also provided are GmSHMT alleles containing mutations R130P and Y358N along with research and breeding methods and compositions including such.

Abstract: This invention provides purified preparations of an RNA, oligoribonucleotide, or polyribonucleotide comprising a modified nucleoside, and methods of assessing purity of purified preparations of an RNA, oligoribonucleotide, or polyribonucleotide comprising a modified nucleoside.

Abstract: Provided herein are compositions, methods, and kits for hematopoietic stem cell induction or for reprogramming cells to the multipotent state of hematopoietic stem cells. In some embodiments, the compositions comprise at least one HSC inducing factor. Such compositions, methods and kits can be used for inducing hematopoietic stem cells in vitro, ex vivo, or in vivo, as described herein, and these induced hematopoietic stem cells can be used in regenerative medicine applications and therapies.

Abstract: The present invention solves the problem of providing more efficient barrier insulators to avoid vector silencing and to increase expression in the setting of gene transfer vectors, more particularly in the setting of gene transfer retroviral vectors. In this sense, the authors of the present invention have developed an improved insulator element, namely element IS2, which comprises the following combination of nucleic acid molecules, namely nucleic acid molecule HS4-650 bp as shown in SEQ ID No 2 and a synthetic S/MAR nucleic acid molecule containing 5 M/SARs recognition signatures (MRS) as shown in SEQ ID no 1.

Abstract: Recombinant viral vectors such as AAV vectors designed with expression cassettes that approach the natural packaging capacity of the virus, such as AAV are provided. The recombinant viral vectors reduce residual plasmid DNA impurities.

Abstract: The present invention provides novel, stable lipid particles having a non-lamellar structure and comprising one or more active agents or therapeutic agents, methods of making such lipid particles, and methods of delivering and/or administering such lipid particles. More particularly, the present invention provides stable nucleic acid-lipid particles (SNALP) that have a non-lamellar structure and that comprise a nucleic acid (such as one or more interfering RNA), methods of making the SNALP, and methods of delivering and/or administering the SNALP.

Abstract: The present invention relates to genetic techniques employing the direct ligatation of an external DNA fragment generated in situ by the same ZFNs that target the genome. ObLiGaRe, i.e., the obligated ligation-gated recombination, is a new method for genetic engineering using custom designed nucleases, and a strategy of site-specific gene insertion utilizing the NHEJ pathway. It applies a similar logic to the one used in unidirectional loxP sites(Oberdoerffer et al., 2003) but maintains all the advantages and flexibility of CDNs.

Abstract: A method of producing a population of differentiated cells comprising: a) inducing differentiation in a first population of cells by applying an inducer to said cells, b) harvesting microvesicles produced from first population of cells, and c) inducing differentiation in a second population of cells by applying said microvesicles or a derivative thereof to said second population of cells wherein, said first population of cells is autologous to said second population of cells and wherein the inducer applied to said first population of cells is not present in said second population of cells or is only present in trace amounts. Also related methods of producing microvesicles, methods of medical and/or cosmetic treatment and related products and uses.

Abstract: The invention features compositions and methods for the increased production of mevalonate, isoprene, isoprenoid precursor molecules, and/or isoprenoids in microorganisms by engineering a microorganism for increased carbon flux towards mevalonate production in the following enzymatic pathways: (a) citrate synthase, (b) phosphotransacetylase, (c) acetate kinase, (d) lactate dehydrogenase, (e) malic enzyme, and (f) pyruvate dehydrogenase such that one of more of the enzyme activity is modulated. In addition, production of mevalonate, isoprene, isoprenoid precursor molecules, and/or isoprenoids can be further enhanced by the heterologous expression of the mvaE and mvaS genes (such as, but not limited to, mvaE and mvaS genes from the organisms Listeria grayi DSM 20601, Enterococcus faecium, Enterococcus gallinarum EG2, and Enterococcus casseliflavus).

Abstract: Fermentation products (e.g., bio-products such as hydrocarbons and other organic compounds) are produced from biomass or gases through digestion and fermentation under conditions that thermodynamically favor production of the fermentation products.

Abstract: The present disclosure generally relates to microorganisms that comprise one or more polynucleotides coding for enzymes in one or more pathways that catalyze a conversion of a fermentable carbon source to butadiene. Also provided are methods of using the microorganisms in industrial processes including, for use in the production of butadiene and products derived therefrom.

Abstract: The present invention relates to a method for generating alkenes through a biological process. More specifically, the invention relates to a method for producing alkenes (for example propylene, ethylene, 1-butylene, isobutylene or isoamylene) from molecules of the 3-hydroxyalkanoate type.

Abstract: The invention relates to processes of culture of a Deinococcus or a related bacterium using an oxidizer in conditions suitable for allowing the growth of Deinococcus and decreasing the growth of at least one other microorganism.

Abstract: The invention relates to the use of EntD polypeptides, polynucleotides encoding the same, and homologues thereof to enhance the production of fatty aldehydes and fatty alcohols in a host cell.

Abstract: The present invention relates to composition and methods of producing bioenergy. More specifically, the invention relates to the use of bacterium of the genus Deinococcus and/or related genera for the modification of biomass or biomass derivatives with a view to producing bioenergy products and metabolites.

Abstract: The present invention pertains to the use of engineered variants of enzyme CYP102A, also known as P450-BM3, for cyclopropanation of olefins containing electron-withdrawing groups. One exemplary enzyme variant, referred to as BM3-HStar, contains five mutations away from wild-type P450-BM3, and demonstrates high activity towards cyclopropanation of olefinic substrates using ethyldiazoacetate (EDA) and other carbene transfer reagents. Products of these reactions are potential precursors of levomilnacipran derivatives, a class of compounds that have been shown to be selective inhibitors of monoamine transporters. In addition, cyclopropanation reactions with the P450-BM3 enzyme variants of the invention can be conducted in whole cells expressing the enzyme variants and can proceed under aerobic conditions.

Abstract: Methods and systems for the biological conversion of pretreated or solubilized coal or waste coal into biofuels. Coal (10) may be pretreated perhaps in a pretreatment reactor (13). Pretreated coal or even solubilized coal may be introduced into a processing reactor such as a bioreactor (16) containing a plurality of microorganisms (9) such as oleaginous microorganisms which can convert at least some of the pretreated or solubilized coal into lipids (19) or biomass (18), which then may be used directly or as a precursor for various products such as biofuels, feedstock, or the like.

Abstract: The present invention relates to variant thioesterases and their use in plants, e.g., to increase enzymatic activity and to promote increased production of mid-chain length fatty acids (e.g., 8 to 14 carbons) and at desired ratios. Further disclosed herein are methods of manufacturing renewable chemicals through the manufacture of novel triglyceride oils followed by chemical modification of the oils. Oils containing fatty acid chain lengths of C8, C10, C12 or C14 are also disclosed and are useful as feedstocks in the methods described herein.

Abstract: A method for converting flue gas into useable bio-oil based compounds using algae includes mixing flue gas with water, infusing the mixture with a lipid trigger and nutrients, distributing the mixture into processing cells, exposing the mixture to light, promoting the algae to the top of the processing cells for harvest, skimming the algae from the surface of the water, and extracting bio-oil from the algae.

Abstract: Provided are methods for synthesizing compounds, including chiral kynurenine compounds. The methods are suitable for large-scale manufacture and produce the chiral kynurenines compounds in high chemical purity and high chiral purity.

Abstract: The present invention relates to a method for the enzymatic synthesis of enantiomerically enriched (R)-amines of general formula [1][c] from the corresponding ketones of the general formula [1][a] by using novel transaminases. These novel transaminases are selected from two different groups: either from a group of some 20 proteins with sequences as specified herein, or from a group of proteins having transaminase activity and isolated from a microorganism selected from the group of organisms consisting of Rahnella aquatilis, Ochrobactrum anthropi, Ochrobactrum tritici, Sinorhizobium morelense, Curtobacterium pusiffium, Paecilomyces lilacinus, Microbacterium ginsengisoli, Microbacterium trichothecenolyticum, Pseudomonas citronellolis, Yersinia kristensenii, Achromobacter spanius, Achromobacter insolitus, Mycobacterium fortuitum, Mycobacterium frederiksbergense, Mycobacterium sacrum, Mycobacterium fluoranthenivorans, Burkhoideria sp., Burkhoideria tropica, Cosmospora episphaeria, and Fusarium oxysporum.

Abstract: The present invention relates to isolated polypeptides having xylanase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

Abstract: A method of producing hyaluronic acid (HA) in Escherichia coli and Bacillus megaterium through episomal plasmid vectors wherein the gene is under the control of strong promoter T7, preferably under the control of strong promoter T7 of bacteriophage T7, and a system for the selection of stable bacterial strains producing high levels of hyaluronic acid, are provided.

Abstract: A fungal alpha-amylase is provided from Aspergillus fumigatus (AfAmyl). AfAmyl has an optimal pH of 3.5 and is operable at 30-75 degrees C., allowing the enzyme to be used in combination with a glucoamylase and an isoamylase in a saccharification reaction. This obviates the necessity of running a saccharification reaction as a batch process, where the pH and temperature must be readjusted for optimal use of the alpha-amylase or glucoamylase. AfAmyl also catalyzes the saccharification of starch substrates to an oligosaccharide composition significantly enriched in DP2 and (DPI+DP2) compared to the products of saccharification catalyzed by an alpha-amylase from Aspergillus kawachii. This facilitates the utilization of the oligosaccharide composition by a fermenting organism in a simultaneous saccharification and fermentation process, for example.