Abstract: The present invention provides phosphorous-containing compounds useful as antiwear additive components, lubricant additive compositions and lubricant compositions each comprising such compounds, and methods for making and using the same.

Abstract: A crude oil friction reducer is disclosed. The friction reducer includes water soluble anionic surfactants as active components and non-aromatic solvents. The water soluble anionic surfactants include anionic sulfonate surfactant and an anionic sulfate surfactant, such as anionic sodium sulfate surfactant, dissolved in an aqueous chelating solution. The friction reducer may also include a pH adjuster. The friction reducer is used with heavy and extra heavy crude oil.

Abstract: An aqueous based metalworking fluid that is environmentally friendly and process for metalworking with the fluid. The metalworking fluid is sampled at periodic intervals and replenished to predetermined operating specifications with the aqueous based metalworking fluid without the need for individual boosters.

Abstract: Corn stillage oil derivatives having values for Gardner color of 10 or less and methods for making the corn stillage oil derivatives are disclosed. In one aspect, the corn stillage oil derivative comprises a heat bleached corn stillage oil. Preferably, the heat bleached corn stillage oil has a value for Gardner color of from 4 to 8. In another aspect, the corn stillage oil derivative comprises a blown corn stillage oil. Preferably, the blown corn stillage oil has a value for Gardner color of from 5 to 8.

Abstract: Blown corn stillage oils and methods for making blown corn stillage oils are disclosed. In one aspect the corn stillage oils are stripped to reduce the acid value of the resulting blown, stripped corn stillage oil. The method includes heating a corn stillage oil to a temperature of at least 90° C., and passing air through the heated oil to produce a blown corn stillage oil having a viscosity of at least 50 cSt at 40° C. In one aspect, the blown corn stillage oil is stripped to reduce the acid value of the blown, stripped corn stillage oil to 5 mg KOH/gram or less.

Abstract: Alkoxylated fatty ester compositions are disclosed. In one aspect, the compositions comprise a reaction product of a metathesis-derived C10-C17 monounsaturated acid, octadecene-1,18-dioic acid, or their ester derivatives with one or more alkylene oxides in the presence of an insertion catalyst to give an alkoxylated fatty ester. In another aspect, the metathesis-derived C10-C17 monounsaturated acid, octadecene-1,18-dioic acid, or its ester derivative is reacted with a glycol ether or a glycol ether alkoxylate, to give an alkoxylated fatty ester. In yet another aspect, the metathesis-derived C10-C17 monounsaturated acid or octadecene-1,18-dioic acid is reacted with one or more alkylene oxides to give a fatty acid alkoxylate, followed by etherification of the fatty acid alkoxylate.

Abstract: Laundry detergents useful for cold-water cleaning and boosted bargain detergents are disclosed. The detergents include a surfactant composition derived from a metathesis-derived C10-C17 monounsaturated acid, octadecene-1,18-dioic acid, or their ester derivatives. For fatty alkyl ester sulfonate-containing detergents, the composition is selected from C10 betaines, C12 or C16 amidoamines, C12 ethanolamine amides, C16 amidoamine sulfonates, C18 diamidoamine dioxides, quaternized C18 diamidoamine betaines, sulfonated C18 low-EO fatty ester alkoxylates, C18 amidoamine carboxylates, and amidoamine oxides and sulfobetaines derived from cross-metathesis of palm or soybean oil. The bargain detergents include a performance booster selected from C12 low-EO fatty ester alkoxylate sulfonates, C18 amidoamine oxide esters, C18 amidoamine oxide carboxylates, and amidoamine sulfobetaines made from self metathesized palm or soybean oil.

Abstract: A fabric enhancer composition comprising at least 0.1% by weight of fabric softening active of formula (A): {[R2]2N+[CH2—CH(CH3)—O—C(?O)—R1][CH(CH3)—CH2—O—C(?O)—R1]}X? wherein each R2 is independently either hydrogen, a short chain C1-C6 alkyl, C1-C3 hydroxyalkyl group, a poly(C2-3 alkoxy), or benzyl, wherein each R1 is independantly a hydrocarbyl group or substituted hydrocarbyl group comprising 11 to 21 carbon atoms, and wherein X? is any fabric enhancer compatible anion. The composition comprises no more than 50% by weight of diester quaternary ammonium compound.

Abstract: Cleaning compositions are provided. The cleaning compositions can include a builder and a dispersant. The cleaning compositions can include one or more additional components. The cleaning compositions can be used in cleaning applications such as automated dishwashing.

Abstract: The present invention provides for the use of activator complexes to enhance lower temperature cleaning in alkaline peroxide cleaning systems. Compositions including at least one of an activator complex, an active oxygen source, and a source of alkalinity are applied to the surface to be cleaned at temperatures between about 5° C. and about 50° C. The methods and compositions of the present invention provide for enhanced soil removal with reduced energy, water, and chemistry consumption.

Abstract: The present invention concerns a method of making manganese ion-containing catalyst salts, which comprise a mononuclear or dinuclear Mn (III) and/or Mn (IV) complex based on cyclic triamine ligands and a non-coordinating silicate-based counterion. The invention also concerns compositions comprising such salts and bleaching formulations comprising the salt and a peroxy compound. The salt, and formulations comprising it, are suitable for use in catalysing oxidation, for example as a component of a laundry or dishwasher bleaching composition. The invention further relates to methods of oxidising with the bleaching formulations described herein.

Abstract: A method for cleaning the glass surface of solar panels which employs the use of highly efficient sequestering agents and allows to replace deionized water with municipal or fresh water of up to a very high water hardness, without having a loss in the long term power output of the solar panels.

Abstract: Articles that include water-soluble films and household care composition, where the films include a polyvinyl alcohol (PVOH) resin blend and optionally one or more additional components such as plasticizers, fillers, surfactants, and other additives. The PVOH resin blend includes a PVOH copolymer including one or more types of anionic monomer units such as a PVOH terpolymer and a PVOH polymer such as another PVOH anionic copolymer or a partially or completely hydrolyzed PVOH homopolymer. When the PVOH copolymer and PVOH polymer are blended in particular proportions and/or selected with regard to various criteria related to physical and chemical film properties, the resulting water-soluble film formed from the PVOH resin blend exhibits substantially improved aqueous dissolution properties, tensile strength properties, and/or tensile modulus properties.

Abstract: Disclosed herein are articles that include water-soluble films, where the water-soluble films include a polyvinyl alcohol (PVOH) polymer and a combination of at least three plasticizers. The combination of plasticizers includes dipropylene glycol as a first plasticizer, a sugar alcohol such as sorbitol as a second plasticizer, and a polyol such as glycerin as a third plasticizer. When the PVOH polymer and plasticizers are blended in particular proportions and/or selected with regard to various criteria related to physical and chemical film properties, the resulting water-soluble film formed from the PVOH resin blend exhibits beneficial combinations four) of aged tensile strength, aged melting transition delta elevation, aged adhesion value, and/or resistance to seal peeling, which provide strong film seals that retain their water-solubility characteristics.

Abstract: A system for incubating microfluidic droplets includes a droplet incubator, a gas exchanger and a conveyor. A method provides homogeneous incubation conditions in a droplet incubator by targeted introduction of gases and/or gas mixtures into a carrier fluid.

Abstract: A hanging drop plate and a method of cultivating cells or of producing molecular aggregates in at least one liquid volume that adheres to a drop contact area of such a hanging drop plate. The hanging drop plate has a body with a first surface and a second surface that is essentially coplanar to the first surface. The second surface has a drop contact area for adherently receiving a liquid volume. The drop contact area is distinguished from a surrounding area by a relief structure that prevents spreading of the liquid volume on the second surface of the body. The body has at least one conduit that mouths into the drop contact area from the direction of the first surface of the body. A liquid volume is applied to the drop contact area through a communicating conduit. Cells and/or molecules can be introduced into this liquid volume.

Abstract: An apparatus for applying an electric field includes: a base, on which an internal region is defined, a plurality of electrodes disposed to face each other in parallel in the internal region of the base, and a sample plate disposed between the plurality of electrodes.

Abstract: Embodiments of a microfluidic system and method for stimulating a blast shock wave that supports a short and defined laminar flow of a liquid media solution through a microfluidic device such that sheer stress on neural tissue disposed within the microfluidic device is precisely controlled are disclosed. The microfluidic system includes a pneumatic device applies a blast shock wave having a quick rise time across a microfluidic channel of the microfluidic device. The microfluidic device includes an inlet reservoir in fluid flow communication with an outlet reservoir through the microfluidic channel. The inlet and outlet reservoirs are secured to a top structure which is attached to a bottom structure that collectively defines the microfluidic channel with a cover slip that is attached to the underside of the bottom structure.

Abstract: Acoustic perfusion devices for separating biological cells from other material in a fluid medium are disclosed. The devices include an inlet port, an outlet port, and a collection port that are connected to an acoustic chamber. An ultrasonic transducer creates an acoustic standing wave in the acoustic chamber that permits a continuous flow of fluid to be recovered through the collection port while keeping the biological cells within the acoustic chamber to be returned to the bioreactor from which the fluid medium is being drawn.

Abstract: Some variations provide an apparatus for enzymatically hydrolyzing pretreated lignocellulosic biomass, including at least one clarifying vessel comprising: an inlet well, disposed with walls at or near the top of the clarifying vessel, configured for continuously feeding pretreated lignocellulosic biomass; overflow weirs to allow liquid-phase hydrolysate to be continuously recovered from the clarifying vessel; and a controllable bottom outlet to allow a solid phase to be continuously or periodically recovered from the clarifying vessel. In certain embodiments, the clarifying vessel is an existing recausticizing clarifier in a pulp and paper mill.

Abstract: Manufactured capillary tubes can include: a tubular member; and a coating applied partially covering the tubular member, the coating defining a window where the tubular member is free of the coating. Kits containing and methods related to the capillary tubes can be used for the cryopreservation of cells.

Abstract: Compositions and methods and apparatus for growth and maintenance of microorganisms and/or bioprocesses using one or more gases as electron donors, electron acceptors, carbon sources, or other nutrients, and for a bioprocess that converts hydrogen and carbon dioxide, or syngas, or producer gas into lipid products, bio-based oils, or other biochemical products.

Abstract: Efficient stem cell delivery into biomaterials using capillary driven encapsulation are disclosed herein where stem/progenitor and/or tissue specific cells are rapidly and efficiently seeded via capillary driven encapsulation into a porous scaffold for cell deliver in the skin or any other organ. The rapid capillary force approach maximizes both seeding time and efficiency by combining hydrophobic, entropic and capillary forces to promote active, ‘bottom-up’ cell engraftment. This methodology uses micro domain patterned biopolymers in a porous dry gel to generate capillary pressure to move a viscous stem cell mix from a hydrophobic reservoir into the polymer matrix to promote active cell seeding within the entire gel volume.

Abstract: Provided herein are methods for the generation of functional keratinocyte stem cells that are differentiated directly from human ESCs/iPSCs in a chemically defined serum-free cell culture system, as well as cells derived therefrom and methods of use thereof. Also provided are methods for culturing primary keratinocytes.

Abstract: The present invention provides methods for the identification, isolation and/or enrichment of human corneal endothelial cells (HCECs). In some embodiments, the method comprises a positive selection process in which a cell population containing human corneal cells is contacted with a positive affinity reagent that selectively binds to HCECs relative to cells other than HCECs (e.g., corneal keratocytes, etc.) in the population and/or a negative selection process in which a cell population containing HCECs is contacted with a negative affinity reagent that selectively binds to cells other than HCECs in the population relative to HCECs. The present invention also provides reagents and kits for the identification, isolation and/or enrichment of HCECs as well as compositions that are enriched in HCECs.

Abstract: Methods are provided for the production of dendritic cells from monocytes that have been incubated at a temperature of 1° C.-34° C. for a period of approximately 6 to 96 hours from the time they are isolated from a subject. After the incubation period, the monocytes can then be induced to differentiate into dendritic cells. Mature dendritic cells made by the methods of the invention have increased levels of one or more of CD80, CD83, CD86, MHC class I molecules, or MHC class II molecules as compared to mature dendritic cells prepared from monocytes that have not been held at 1° C.-34° C. for at least 6 hours from the time they were isolated from a subject. Dendritic cells made by the methods of the invention are useful for the preparation of vaccines and for the stimulation of T cells.

Abstract: The present invention relates to the generation of a chondrocyte using mesenchymal stem/progenitor cells obtained from the amniotic membrane of umbilical cord and therapeutic uses of such chondrocytes.

Abstract: Apparatuses, systems, and methods for generating concentration gradients of soluble molecules are disclosed herein. Devices and methods for generating in vitro blood vessels are also disclosed.

Abstract: The present invention relates to endocardium-derived adult stem cells obtained by culturing peripheral blood mononuclear cells (PBMCs) separated from peripheral blood, and a cell therapeutic agent for treating cardiovascular diseases containing the same as an active ingredient. The adult stem cells, according to the present invention, have an origin that is the endocardium and strong blood vessel formation, and is thus remarkably useful for treating cardiovascular diseases such as ischemia, myocardial infarction, and the like.

Abstract: The present invention provides a method of altering the specificity of an adenovirus vector. The method involves providing an adenovirus having a capsid with a modified adenovirus hexon protein. The modified adenovirus has a capsid comprising a hexon protein with a deletion in hypervariable region 1 and/or hypervariable region 4 of the hexon and an insert of an exogenous molecule therein.

Abstract: The invention relates to the discovery of an HEV strain from a chronically infected patient. The virus grow unusually well in numerous cell cultures. Thus, the invention provides cell cultures, vectors, and vaccine compositions based on the virus.

Abstract: This invention relates to modified parvovirus inverted terminal repeats (ITRs) that do not functionally interact with wild-type large Rep proteins, synthetic Rep proteins that functionally interact with the modified ITRs, and methods of using the same for delivery of nucleic acids to a cell or a subject. The modifications provide a novel Rep-ITR interaction that limits vector mobilization, increasing the safety of viral vectors.

Abstract: The present disclosure is directed to glycosyltransferase variants having N-terminal truncation deletions. Contrary to previous findings certain truncations comprising the conserved amino acid motif (“QVWxKDS”) were found to be compatible with glycosyltransferase enzymatic activity, particularly in a human sialyltransferase (hST6Gal-I). Thus, disclosed are variants of mammalian glycosyltransferase, nucleic acids encoding the same, methods and means for recombinantly producing the variants of mammalian glycosyltransferase and use thereof, particularly for sialylating terminal acceptor groups of glycan moieties being part of glycoproteins such as immunoglobulins.

Abstract: A polypeptide which is a mutated mannanase, whose sequence is derived from a native mannanase of the filamentous ascomycete coprophile fungus Podospora anserina, and being characterised by an increased catalyst efficiency of at least 25%. Also, a polynucleotide encoding the polypeptide, a vector including the polynucleotide, a host cell including the vector or the polynucleotide, and a composition including the polypeptide, the polynucleotide, the vector or the host cell. Methods of using the polypeptide, the polynucleotide, the vector, the host cell or the composition of the invention for degradation of compounds including mannans, in particular lignocellulosic biomass, as well as various possible uses of the polypeptide, the polynucleotide, the vector, the host cell or the composition.

Abstract: The present invention relates to immobilized proline-specific endoprotease, wherein the proline-specific endoprotease is immobilized on a carrier comprising methacrylate which has been functionalized with amino dimethylene, and wherein the carrier has a particle size range of 100 to 400 ?m. The invention also relates to a process for producing beer comprising the steps of preparing a mash, fermenting the beer, and stabilizing the beer, wherein the beer is incubated with the immobilized proline-specific endoprotease.

Abstract: The present invention relates to a method for producing a protein, comprising an inspection process, wherein the inspection process comprises: a step of approximating an infrared absorption band derived from a protein appearing around 1500 to 1600 cm?1 or around 1600 to 1700 cm?1 in an infrared absorption spectrum of the protein, by one or more normal distributions, a step of calculating an index value indicating a degree of broadening of the infrared absorption band based on the normal distributions, and a step of comparing the index value with a predetermined threshold to select, as a good-quality product, a protein having a degree of broadening of the infrared absorption band that is smaller than the threshold.

Abstract: A composite material includes: an apatite crystal in the form of a tube; and a functional component accommodated in the apatite crystal tube and constituted by a material having physical properties different from those of the apatite crystal. The apatite crystal may be a monocrystal given by the general formula M25(PO4)3X, where M2 denotes at least one element selected from the group consisting of divalent alkali earth metals and Eu, and X denotes at least one element or molecule selected from the group consisting of halogens and OH.

Abstract: Disclosed herein is a method for isolating a nucleic acid from a sample. The method includes contacting the sample with boron carbide under conditions sufficient to form a boron carbide-nucleic acid complex. The complex is separated from the sample.

Abstract: The present invention concerns the use of methods and compositions for the isolation of small RNA molecules (100 nucleotides or fewer), such as microRNA and siRNA molecules. Such molecules are routinely lost in commonly used isolation procedures and therefore the present invention allows for a much higher level of enrichment or isolation of these small RNA molecules.

Abstract: Methods for processing polynucleotide-containing biological samples, and materials for capturing polynucleotide molecules such as RNA and/or DNA from such samples. The RNA and/or DNA is captured by polyamindoamine (PAMAM (Generation 0)) bound to a surface, such as the surface of magnetic particles. The methods and materials have high efficiency of binding RNA and of DNA, and of release, and thereby permit quantitative determinations.

Abstract: Compositions and methods are provided for enzymes with altered properties that involve a systematic approach to mutagenesis and a screening assay that permits selection of the desired proteins. Embodiments of the method are particularly suited for modifying specific properties of restriction endonucleases such as star activity. The compositions includes restriction endonucleases with reduced star activity as defined by an overall fidelity index improvement factor.

Abstract: Provided are LMNA-targeted antisense oligonucleotides for reducing expression of one or more aberrantly spliced LMNA mRNA isoforms that encode progerin.

Abstract: Oxidative damage contributes to cone cell death in retinitis pigmentosa and death of rods, cones, and retinal pigmented epithelial (RPE) cells in ocular oxidative stress related diseases including age-related macular degeneration and retinitis pigmentosa. Oral antioxidants may provide modest benefits, but more efficient ways of preventing oxidative damage are needed. Compositions and methods are provided herein for the prevention, amelioration, and/or treatment of early or late stage ocular disease by increasing the expression or activity of one or more peroxidases in cells of the eye, particularly retinal cells, and further optionally increasing the expression or activity of one or more superoxide dismuatases in the same cells.

Abstract: The invention provides engineered RNA precursors that when expressed in a cell are processed by the cell to produce targeted small interfering RNAs (siRNAs) that selectively silence targeted genes (by cleaving specific mRNAs) using the cell's own RNA interference (RNAi) pathway. By introducing nucleic acid molecules that encode these engineered RNA precursors into cells in vivo with appropriate regulatory sequences, expression of the engineered RNA precursors can be selectively controlled both temporally and spatially, i.e., at particular times and/or in particular tissues, organs, or cells.

Abstract: The present invention provides novel medical means to facilitate glutathione (GSH) synthesis in the brain. These means are miR-96-5p inhibitor increasing GSH expression in the brain and a pharmaceutical composition comprising the miR-96-5p inhibitor and having a preventive and/or therapeutic performance to a disease caused by decrease of GSH amount or depression of GSH activity.

Abstract: The present invention relates to methods for protecting against damage caused by radiation and/or chemotherapy, and methods for treating bone marrow damage by reducing/inhibiting Latexin expression and/or Latexin activity. The methods comprise administering to a subject in need thereof a pharmaceutical composition comprising an antagonist that reduces expression and/or activity of latexin, wherein latexin is a latexin polynucleotide variant and/or a latexin polypeptide variant that binds to the antagonist.

Abstract: The invention relates to a double-stranded ribonucleic acid (dsRNA) for inhibiting the expression of the HAMP gene (HAMP gene), comprising an antisense strand having a nucleotide sequence which is less that 30 nucleotides in length, generally 19-25 nucleotides in length, and which is substantially complementary to at least a part of the HAMP gene. The invention also relates to a pharmaceutical composition comprising the dsRNA together with a pharmaceutically acceptable carrier; methods for treating diseases caused by HAMP gene expression and the expression of the HAMP gene using the pharmaceutical composition.

Abstract: The present invention relates to compounds, pharmaceutical compositions comprising same, methods of use thereof and kits for the down-regulation of RhoA gene. The compounds, compositions, methods and kits are useful in the treatment of subjects suffering from diseases or conditions and or symptoms associated with diseases or conditions in which RhoA expression has adverse consequences and for conferring neuroprotection.

Abstract: Provided herein are exogenous terminator sequences for use in fungi cell transcriptional termination.

Abstract: The present invention relates to methods of producing a polypeptide, comprising: (a) cultivating a mutant of a parent Fusarium venenatum strain in a medium for the production of the polypeptide, wherein the mutant strain comprises a polynucleotide encoding the polypeptide and one or more (several) genes selected from the group consisting of pyrG, amyA, and alpA, wherein the one or more (several) genes are modified rendering the mutant strain deficient in the production of one or more (several) enzymes selected from the group consisting of orotidine-5?-monophosphate decarboxylase, alpha-amylase, and alkaline protease, respectively, compared to the parent Fusarium venenatum strain when cultivated under identical conditions; and (b) recovering the polypeptide from the cultivation medium. The present invention also relates to enzyme-deficient mutants of Fusarium venenatum strains and methods for producing such mutants.