Abstract: An electrochemical test strip, measurement system and method for determining sample content in the reactive region of the electrochemical test strip are presented. The electrochemical test strip comprises an insulator substrate which includes an electrode system disposed thereon. The electrode system includes at least one pair of electrodes. A lower plate which includes a reactive region and a sampling cell is disposed on the insulator substrate. An upper plate is disposed on the lower plate, wherein the at least one pair of electrodes is designed as an electrical loop adjacent to the sampling port.

Abstract: The present invention announces a method for extending the storage life of a test reagent for quantification of ionic components in samples by using an anion sensor. The purpose of the present invention is to provide an anion sensor, wherein the storage life of the anion sensor itself may be extended, to thereby extend the storage life of the anion sensor beyond that of conventional anion sensors. According to the present invention, through adjustment of the pH of the internal solution (internal gel liquid), it is possible to provide an anion sensor that maintains consistent performance, and has longer storage life.

Abstract: Disclosed herein are systems and methods that relate to use of ultramicroelectrodes (UME) in measurement of ion concentration in presence of one or more organic compound(s).

Abstract: A floating gate based sensor apparatus includes at least two a separate electrical bias components with respect to a floating gate based sensor surface within the floating gate based sensor apparatus. By including the at least two electrical bias components, the floating gate based sensor apparatus provides enhanced capabilities for biomaterial and non-biomaterial detection and manipulation while using the floating gate based sensor apparatus.

Abstract: In order to improve measurement accuracy by accurately calibrating sensitivity of a dissolved oxygen meter without impairing easiness in calibration, the present invention includes a dissolved oxygen measuring device that includes a cell to be loaded with a sample solution, and a dissolved oxygen meter that is attached to the cell and measures an oxygen concentration in the sample solution; and a mode switching mechanism to switch between a measurement mode, in which a sensor surface of the dissolved oxygen meter is substantially in contact with the sample solution in the cell, and a calibration mode, in which the sensor surface is substantially not in contact with the sample solution in the cell, by feeding air into the cell through a feed port disposed on the cell, and by discharging part of the sample solution through a discharge port disposed on the cell.

Abstract: Quartz crystal microbalance resonators are described, as are methods of making them using micron sized pillar array of polymethyl methacrylate fabricated on a QCM surface using a nanoimprint lithography process. Their use in any applications, including for example gas and liquid sensors and biosensors as well as other measurement applications, is also discussed.

Abstract: The present invention relates to a method for determination of pollutants and leachables in dialysis solutions by stir bar sorptive extraction comprising the steps of a. Conditioning of the stir bar coated with a sorptive material b. Stirring the dialysis solution with a coated stir bar c. Desorption of pollutants and leachables from the coated stir bar.

Abstract: Apparatus and method measure the flow rate of fluid for use in calibrating fraction collection in a chromatography system. An injector inserts a detectable substance into a flow of fluid. A first detector, disposed in a path of the flow of liquid with the substance, produces a signal in response to detecting the substance in the flow of fluid. A second detector, disposed downstream from the first detector in the path of the flow of fluid with the substance, produces a signal in response to detecting the substance in the flow of fluid. A computing system receives each signal produced by the first and second detectors upon detecting the substance in the flow of fluid, and computes a volumetric flow rate of the flow of fluid based on a time interval between the signals and a volume of the path between the first and second detectors.

Abstract: Disclosed is an extraction and analysis system and method. The system comprises a separation apparatus, a liquid-liquid extraction apparatus and an analysis apparatus. The liquid-liquid extraction apparatus is configured to receive a substantially continuous inlet flow of a first liquid from the separation apparatus, the first liquid carrying a mixture of components including an analyte. The liquid-liquid extraction apparatus is operable to receive a second liquid for contact with the first liquid, the second liquid being substantially immiscible with the first liquid. This provides partitioning of the analyte and/or another component carried by the first liquid preferentially into the second liquid substantially continuously. Subsequently the first and second liquids are separated substantially continuously.

Abstract: A multi-dimensional liquid analysis system includes a flow splitter for separating mobile phase outflow from a first dimension liquid analysis system into fist and second liquid split outlet flows. Volumetric flow rate control of the split outlet flows is provided by a flow control pump which withdraws one of the split outlet flows from the flow splitter at a controlled withdrawal flow rate to define the other split outlet flow rate as the difference between the outflow rate from the first dimension system and the withdrawal flow rate. In this manner, accurate and consistent flow division can be accomplished, which is particularly useful for multi-dimensional liquid analysis.

Abstract: A microfluidic liquid chromatography-electrospray ionization (LC-ESI) device is provided for enhancing the sensitivity of mass spectrometric detection of an analyte in a sample. The device is designed to drive effective intermixing of an analytical flow stream exiting a chromatographic stationary phase and a post-column modifier reagent. The mixed flow stream thus obtained is used for generating an electrospray containing analyte ions. Also provided are methods for enhanced sensitivity of detection of an analyte in a sample.

Abstract: The invention generally relates to ion generation using modified wetted porous materials. In certain aspects, the invention generally relates to systems and methods for ion generation using a wetted porous substrate that substantially prevents diffusion of sample into the substrate. In other aspects, the invention generally relate to ion generation using a wetted porous material and a drying agent. In other aspects, the invention generally relates to ion generation using a modified wetted porous substrate in which at least a portion of the porous substrate includes a material that modifies an interaction between a sample and the substrate.

Abstract: A sensing device for determining the presence of hydrogen peroxide is disclosed. The sensing device comprises a light guide having a distal portion terminating at a light guide tip and a proximal portion, wherein the light guide tip is proximal to a layer of Prussian blue (PB), which is pure (or essentially free of contaminants). The sensing device produces a fast linear response and is durable, due to the robustness and purity of the PB. The PB is chemically deposited proximal to, or on, the light guide tip using a single-source precursor technique. Methods of making and using the device also are disclosed herein.

Abstract: Optically interacting electromagnetic radiation with a flowing atmospheric air composition and optically interacting the electromagnetic radiation with an integrated computational element (“ICE”), the ICE being configured to analyze for a contaminant in the flowing atmospheric air. A detector receives the electromagnetic radiation that has optically interacted with the flowing atmospheric air and the ICE and generates an output signal corresponding to a characteristic of the contaminant in the flowing atmospheric air.

Abstract: Provided is a carbon dioxide ground leakage monitoring system that is low cost, capable of real-time multi-point monitoring on a wide scale, and capable of providing the public with easy-to-understand information. A plurality of monitoring posts 100 and a field data server 200 and the field data server 200 and an administrative server 300 are connected by communication lines in respective sections therebetween. The plurality of monitoring posts 100 measure a concentration of carbon dioxide having leaked above the ground, and transmits the measured carbon dioxide data to the field data server 200, the field data server 200 transmits the data transmitted from the plurality of monitoring posts 100 to the administrative server 300, and the administrative server 300 discloses carbon dioxide ground leakage information on the Internet based on the carbon dioxide data transmitted from the field data server 200.

Abstract: A method of calibrating a biological detector can include training a biological detector to detect a calibration compound, wherein the calibration compound comprises an odor that is detectable by the biological detector and is not used in an environment for which the biological detector is trained to detect odors, presenting the biological detector with a device comprising the calibration compound, and determining whether the biological detector will alert to the calibration compound.

Abstract: The disclosure relates to application of a signature elemental tracer referred to herein as a signature). It comprises a selection of a small amount of a unique combination of a non-radioactive isotope or variant of a particular element or elements with stable isotopes, different from the natural-occurring combination of the elements in question. In particular, there are 54 such stable multi-isotopic elements and 17 rare-earth elements available for the creation of signature. The signature can be chosen based on samples of traced material and the environment it is to be used in, over time based on detectability, duration, and robustness in that environment. By utilizing isotope combinations as a signature, one can readily determine with a mass spectrometer, whether the traced material in question was or was not in a particular sample of contamination, plant tissue, animal tissue, or associated with some event of interest.

Abstract: A measuring system (10) and method measure a concentration of components of a gas mixture of gas/aerosol. A reaction support (14) has a flow channel (42) that forms a reaction chamber (46) with an optically detectable reactant (48) that reacts with at least one component or with a reaction product of the component. The flow channel (42) is at least partially filled with particles (100, 102, 104, 110) which have a pre-flow starting position and to which a gas flow is applied through the flow channel (42) in a flow position. The particles (100, 102, 104, 110) are designed (configured) in such a manner that the particles (100, 102, 104, 110) in the starting position and the particles (100, 102, 104, 110) in the flow position can be optically distinguished. The invention also relates to an optical flow sensor (109) for determining a flow of a fluid.

Abstract: A method of evaluating stabilization fluids for use in a subterranean formation having a clay composition is disclosed. The method features providing a plurality of replicant core samples representative of the composition of a subterranean formation. A plurality of stabilization fluids are introduced to the plurality of replicant core sample such that an eluted mixture is extracted from a second end of each replicant core sample. Afterwards, at least one property of the eluted mixture from each replicant core sample is determined.

Abstract: A process for detecting oil or lubricant contamination in a manufactured product, the process comprising adding at least two fluorescent taggants to oils or lubricants contained in processing machinery for said product, irradiating said product, causing at least one of said taggants to fluoresce and detecting radiation emitted by said fluorescing taggant in an oil-contaminated product.

Abstract: Embodiments of the disclosure can include systems, methods, and devices for determining saturation pressure of an uncontaminated fluid. Downhole saturation pressure measurements and downhole OBM filtrate contamination of a contaminated fluid may be obtained and a relationship may be determined between the saturation pressure measurements and OBM filtrate contamination. The relationship may be extrapolated to zero OBM filtrate contamination to determine the saturation pressure of the uncontaminated fluid. In some embodiments, OBM filtrate contamination may be determined from downhole saturation pressure measurements during pumpout of a fluid.

Abstract: Method for characterizing the hydrocarbon content of a reformate sample stream. Water and ammonia are removed from the sample stream and the hydrocarbon content is measured using a chemical component analyzer.

Abstract: A method for determining a salt concentration of a hydrocarbon fluid using a conductivity sensor and a hydrocarbon testing solution includes forming the hydrocarbon testing solution. The electropolymerizable monomer is operable to form a resultant associated polymer at a peak potential of the electropolymerizable polymer. The method includes introducing the hydrocarbon testing solution into the conductivity sensor. The method includes inducing a range of potential across the hydrocarbon testing solution such that at least a portion of the electropolymerizable monomer polymerizes. The range of induced potential includes the peak potential of the electropolymerizable polymer. The method includes detecting a range of electrical current associated with the range of potential induced. The method also includes the step of determining the salt concentration of the hydrocarbon fluid using the range of potential induced and the range of electrical current detected.

Abstract: A device for determining the temperature of a road building material applied by a construction machine in a mounting width is arranged at the construction machine in a range within the mounting width and has an infrared temperature measuring head, a motor and a controller, the infrared temperature measuring head being arranged to be twistable by the motor transverse to the direction of travel of the construction machine and being effective to record temperature measuring values of the surface of road building material during a rotational movement at at least two measuring points spaced apart from one another. The controller is effective to control the motor based on the fitting position of the device at the construction machine such that the distance between the measuring points on the surface to be measured remains equal.

Abstract: The present disclosure shows, unexpectedly, that variations in cell culture performance in large-scale cell culture systems such as, for example, those used in commercial manufacturing processes, in some instances, can be attributed to often subtle variations among shear-protectant additives used during cell culture. Assessing the quality of shear-protective additives using such large-scale systems, however, is inaccurate, time-consuming and costly. To solve the problem identified, the present disclosure provides methods and compositions for evaluating the suitability of shear-protectant additives without resorting to large scale cell growth and/or protein production tests.

Abstract: A combination of mutually exclusive cell-based analytical techniques can be applied to the same group of cells for analysis. The same group of cells can be prepared for analysis by each technique resulting with candidate cells targeted for mass cytometry analysis. This configuration allows for the correlation of the information between each technique to produce a matrix of multi dimension of cellular information with the same group of cells.

Abstract: An integrated system for detecting safety and/or efficacy issues related to potential drug compounds by combining tissue maturation, imaging and electrophysiology measurements, at high throughput, with high signal to noise ratio. Testing includes cardiotoxicity screening, drug screening, and screening for cardiogenic factors via on-line physiological measurements.

Abstract: A microfluidic device includes a first microfluidic channel comprising a side wall and an electrode, disposed on the side wall. The microfluidic device further includes an intersection of the first microfluidic channel and a second microfluidic channel proximate to the electrode. The electrode is suspended into an interior region of the first microfluidic chamber.

Abstract: This document provides methods and materials related to assessing immunity to folate receptors. For example, methods and materials for assessing FR? immunity in a mammal are provided. This document also provides methods and materials related to stimulating immunity to folate receptors.

Abstract: The present invention relates to a novel antigen binding protein, in particular a monoclonal antibody, capable of binding specifically to the protein Axl as well as the amino and nucleic acid sequences coding for said protein. From one aspect, the invention relates to a novel antigen binding protein, or antigen binding fragments, capable of binding specifically to Axl and, by inducing internalization of Axl, being internalized into the cell. The invention also comprises the use of said antigen binding protein as an addressing product in conjugation with other anti-cancer compounds, such as toxins, radio-elements or drugs, and the use of same for the treatment of certain cancers.

Abstract: Described herein are particular embodiments relating to a microfluidic device that may be utilized for cell sensing, counting, and/or sorting. Particular aspects relate to a microfabricated device that is capable of differentiating single cell types from dense cell populations. One particular embodiment relates a device and methods of using the same for sensing, counting, and/or sorting leukocytes from whole, undiluted blood samples.

Abstract: This invention provides compound having a structure of Formula I: Uses of such compounds and compositions comprising the compounds as alkylating agent sensors.

Abstract: A grating pattern element includes peaks which are periodically arranged and valleys provided between the peaks, and at least one of the peaks and at least one of the valleys includes an adsorbing material on a surface thereof which adsorbs a target material.

Abstract: The present invention provides methods for diagnosing a disease state in a patient by detecting the presence, expression level and/or activation level of a target analyte in a patient sample using a proximity dual detection assay on a microcarrier. The present invention also provides an assay device for performing the methods described herein.

Abstract: The present invention relates to a method for the detection of a protein including a histidine-tag using immunochromatography. The detection method of the present invention uses immunochromatography using the polymer particle fixed with the histidine-tag, wherein a histidine-tag specific antibody alone is used to detect a histidine-tag conjugated protein (recombinant protein) quickly without using a target protein specific antibody. This method is also efficient in detecting and quantifying the histidine-tagged protein included in the sample without using an additional apparatus but quickly and accurately.

Abstract: This invention describes a device for assaying a dry or small volume liquid sample on a lateral flow capillary assay by utilizing a supplementary non-sample aqueous solution that will push the sample up the assay without diluting or significantly diluting the original sample. This invention enables a sample to be assayed rapidly and/or on-site when the sample is in dried or solid form, or when the liquid volume is too small to complete an assay. As an option, the device can display a colored signal when a sufficient amount of liquid sample has been collected or added to the device. The minimal liquid volume requirement of the device significantly reduces the sample collection time to a matter of seconds and resolves situations in which fluids available for collection are not abundant. Therefore, this invention allows the lateral flow test device to be a more rapid, efficient, and versatile apparatus.

Abstract: Disclosed herein are methods of treatment of autoimmune diseases such as systemic lupus erythematosus (SLE) as well as clinical assays for detection of autoimmune disease activity in patients utilizing a PD1 ligand.

Abstract: Compositions and methods for diagnosing an increased risk of NPSLE are provided.

Abstract: A method of determining the P. aeruginosa or related species infection status of a subject comprising: (i) providing a sample of material obtained from the subject; (ii) determining the level of one or more alkyl quinolones or metabolites thereof produced by P.

Abstract: The present disclosure provides for methods of diagnosing and treating bacterial infections. Human Intelectin 1 (hIntL-1) has been shown to bind selectively to glycan components on bacteria including Streptococcus pneumonia, Proteus mirabilis, Proteus vulgaris, Klebsiella pneumonia and Yersinia pestis. This interaction can be targeted to identify, purify and therapeutically target such organisms.

Abstract: The present disclosure relates to compositions, kits, and methods for the detection of a virus in a sample. Typically, the sample is from a subject suspected of, exhibiting symptoms of, or previously diagnosed with a viral infection, such as an Ebola virus.

Abstract: Polypeptide substrates based on modifications or fragments of the various APP isoforms, assay methods based on the use of these substrates, and screening methods directed toward identifying inhibitors of ?-secretase activity. The assay methods and the screening methods are adapted for use in high throughput multi-well plate assay apparatuses. In many embodiments the substrate polypeptides are labeled for ease of detection, and/or may bind specific ligands that themselves are labeled. Generally the labels promote high specificity as well as high sensitivity of detection. These features render the assay and screening methods that employ the labeled substrates especially suited for use in high throughput assay formats. This disclosure further identifies small polypeptides based on a subsequence motif of A? that are shown herein to be potent inhibitors of the activity of ?-secretase.

Abstract: The present invention provides methods for determining the endotoxin content of an aluminium salt for use in medicine, comprising the steps of: a. Mixing the aluminium salt with a desorption buffer thereby desorbing any endotoxin from the aluminium salt; b. Separating the aluminium salt from the endotoxin; and c. Measuring the amount of endotoxin.

Abstract: The present disclosure provides methods, kits, and compositions for detecting subject anti-HCV antibodies in a sample using NS3 capture peptides. In certain embodiments, at least two NS3 helicase (NS3h) capture peptides and at least two conjugate peptides (e.g., NS3h conjugate peptides) are employed together, which allows for a broad dynamic range of subject antibody detection in a one-step type assay. In other embodiments, methods are provided of detecting NS3-specific subject antibodies without the use of a reducing agent. In some embodiments, NS3-specific subject antibodies are detected with a ‘double shot’ of NS3 conjugate peptide (e.g., conjugate peptide added to a sample both before and after washing).

Abstract: The invention generally provides improved compositions and methods for detecting, diagnosing, prognosing, and monitoring multiple myeloma, chronic lymphocytic leukemia, or B-cell non-Hodgkin lymphoma in a subject. In particular, the invention provides methods for detecting BCMA in subjects to reliably diagnose, predict survival, or monitor multiple myeloma, chronic lymphocytic leukemia, or B-cell non-Hodgkin lymphoma in the subject.

Abstract: The present invention relates to methods for the stratification of a multiple myeloma (MM) patient comprising determining whether or not B-cells, preferably malignant B-cells of said patient express BCMA protein on their surface. Also, methods for selecting an antibody-based multiple myeloma (MM) therapy is based on whether or not BCMA is expressed on the cell surface of B-cells, preferably malignant B-cells of a patient. Furthermore, antibody-based therapies for patients who have BCMA positive malignant B-cells are provided.

Abstract: Newly identified proteins as markers for the detection of colon, ovary, kidney, esophagus and prostate tumors, or as therapeutic targets for their treatment; affinity ligands and particularly antibodies capable of selectively interacting with the tumor markers and methods for tumor diagnosis and thereapy using such antibodies.

Abstract: The present invention relates to methods, kits, and compositions for detecting and/or diagnosing metastatic potential of cancer cells or for evaluating prognosis in a patient with cancer by detection of the protein expression level of an HLA class I molecule and/or the copy number variation of a polynucleotide encoding the HLA class I molecule. The present invention also relates to the use of the protein expression level of an HLA class I molecule and/or the copy number variation of a polynucleotide encoding the HLA class I molecule as a prognosis biomarker and metastasis predictive biomarker of cancer.

Abstract: The present invention provides simple, rapid methods and procedures for analyzing cells, hereunder quantitative and qualitative assessment of cells, such as viability. The present invention relates to the use of various optionally substituted reporter compounds particularly detectable upon their reaction with thiol-containing species present in higher concentrations in intact (e.g., living) cells than in non-intact (e.g., dead, stressed and apoptotic) cells. The present invention also relates to the use of various optionally substituted reporter compounds particularly detectable upon their reaction with species present in intact and/or non-intact cells. Moreover, the present invention relates to the use of measuring techniques and/or instruments coupled with the use of various optionally substituted reporter compounds. The invention further relates to compositions used in methods for analyzing cells, such as a composition comprising N-(7-dimethylamino-4-methyl-3-coumarinyl)-maleimide (DACM).

Abstract: This disclosure provides semiconducting polymer dots (Pdots) for use in a wide variety of applications. In particular, this disclosure provides Pdots that are halogenated, including fluorinated Pdots. This disclosure also provides methods for synthesizing Pdots and methods for using Pdots, such as for biological imaging.