Abstract: Filtering small nucleic acids using permeabilized cells and methods for using the filtering to detect genomic DNA accessibility are described.

Abstract: This invention provides a process for making 3?-O-allyl-dGTP-PC-Biodopy-FL-510, 3?-O-allyl-dATP-PC-ROX, 3?-O-allyl-dCTP-PC-Bodipy-650 and 3?-O-allyl-dUTP-PC-R6G, and related methods.

Abstract: The disclosure provides for a gel extraction device comprising multifunctional and reusable forceps, a removable cutting element, and a sampler component. The disclosure further provides for a freeze/thaw method which can be used to recover biomolecules from agar or agarose gels.

Abstract: A reaction container includes a base material, an analysis well in which a nucleic acid analysis reagent used for a nucleic acid analysis is positioned, the analysis well being arranged on the base material and configured to analyze a sample containing a nucleic acid, and a quantification well in which a quantification reagent configured to specifically detect the nucleic acid is positioned, the quantification well being arranged on the base material and configured to quantify an amount of the nucleic acid contained in the sample, in which the nucleic acid contained in the sample is distributed into the quantification well and the analysis well.

Abstract: Disclosed is a method for the in vitro detection of strains of Legionella pneumophila, resistant to antibiotics, in particular to the fluoroquinolones. Also disclosed are nucleotide probes and a kit of reagents allowing the detection of Legionella pneumophila bacterial strains that are resistant to antibiotics, in particular of the fluoroquinolone type.

Abstract: The present invention provides nucleic acid products and corresponding methods for screening a biological sample for the presence of an E. coli strain causing an infection.

Abstract: Provided herein are compositions and systems for use in polymerase-dependent, nucleotide transient-binding methods. The methods are useful for deducing the sequence of a template nucleic acid molecule and single nucleotide polymorphism (SNP) analyses. The methods rely on the fact that the polymerase transient-binding time for a complementary nucleotide is longer compared to that of a non-complementary nucleotide. The labeled nucleotides transiently-binds the polymerase in a template-dependent manner, but does not incorporate. The methods are conducted under any reaction condition that permits transient binding of a complementary or non-complementary nucleotide to a polymerase, and inhibits nucleotide incorporation.

Abstract: Methods, compositions, and kits are provided for quantification of genome editing.

Abstract: This invention provides novel compositions and processes for analyte detection, quantification and amplification. Nucleic acid arrays and libraries of analytes are usefully incorporated into such compositions and processes. Universal detection elements, signaling entities and the like are employed to detect and if necessary or desirable, to quantify analytes. Amplification of target analytes are also provided by the compositions and processes of this invention.

Abstract: The invention comprises suppressor oligonucleotides for reducing amplification of a non-target nucleic acid sequences; the method of designing and using such oligonucleotides, as well as kits and reaction mixtures.

Abstract: Described herein are methods useful for incorporating one or more adaptors and/or nucleotide tag(s) and/or barcode nucleotide sequence(s) one, or typically more, target nucleotide sequences. In particular embodiments, nucleic acid fragments having adaptors, e.g., suitable for use in high-throughput DNA sequencing are generated. In other embodiments, information about a reaction mixture is encoded into a reaction product. Also described herein are methods and kits useful for amplifying one or more target nucleic acids in preparation for applications such as bidirectional nucleic acid sequencing. In particular embodiments, methods of the invention entail additionally carrying out bidirectional DNA sequencing. Also described herein are methods for encoding and detecting and/or quantifying alleles by primer extension.

Abstract: Disclosed are methods for shearing and tagging chromatin DNA. The disclosed methods include contacting chromatin DNA with at least one transposome, that includes a transposase enzyme. The transposon is made up of a first DNA molecule that includes a first transposase recognition site and a second DNA molecule that includes a second transposase recognition site, wherein the transposase integrates the first and second DNA molecules into chromatin DNA. The first and second DNA molecules of the transposon can be disconnected, such that upon integration of the transposon the chromatin bound DNA is sheared and tagged with the first and second DNA molecules, for example to prepare a library of sheared and tagged chromatin DNA fragments.

Abstract: The invention relates to an additive which can be added to buffers used in nucleotide detection processes and improved methods of nucleic acid sequencing using this additive. In particular the invention relates to use of the additive to improve the efficiency of fluorescence-based multiple cycle nucleic acid sequencing reactions.

Abstract: Provided are methods, kits, and systems useful in the performance of analysis and reporting of highly polymorphic loci, including, in particular, the performance of analysis and reporting of HLA typing. Combining one-step sequencing and sequence-specific oligonucleotide probe hybridization, the methods, kits, and systems offer improved efficiency of HLA typing while providing detailed sequencing information. In certain embodiments the methods and systems comprise one or more software applications to facilitate data acquisition, processing, and reporting.

Abstract: The purpose of the present invention is to provide a method and kit for highly precise DNA typing, in which ambiguity derived from phase ambiguity is eliminated. The present invention provides a method for the DNA typing of HLA, which is characterized by comprising: (1) a step of preparing a set of primers which can respectively anneal specifically to an upstream region and a downstream region of each of HLA-A, HLA-B, HLA-C, HLA-DQA1, HLA-DQB1, HLA-DPA1 and HLA-DPB1 gene in the nucleotide sequence for the human genome, and a set of primers which can respectively anneal specifically to exon-2 and a 3?-side non-translated region in HLA-DRB1; (2) a step of carrying out the PCR amplification of a sample to be tested (DNA) using the sets of primers; (3) a step of determining the nucleotide sequence for a PCR-amplified product; and (4) an optional step of carrying out the homology search in a data base.

Abstract: Methods, systems, and diagnostic tests, including test kits for assessing an offspring's risk of developing a disease or condition known or suspected to have a causal or contributing relationship to an age related epigenetic event in a paternal germ line are disclosed and described

Abstract: Gene expression signature predictive of cancer patient response to multi-kinase inhibitor is disclosed. Also disclosed are methods predicting the efficacy of the multi-kinase inhibitor for treating cancer in a patient. Also disclosed are methods for distinguishing responders from non-responders to a multi-kinase inhibitor in treating cancer. Also disclosed are methods for treating a cancer patient with a multi-kinase inhibitor.

Abstract: The present invention relates to biomarker genes for diagnosing and treating diseases. Provided herein are systems and methods of diagnosing a disease in a patient based on the patient's expression levels of biomarker genes. Examples of the TL1A-associated disease include, but are not limited to, an inflammatory bowel disease (IBD), Crohn's disease (CD), ulcerative colitis (UC), and fibrosis. Also provided herein are systems and methods of identifying a patient likely to be responsive to an anti-TL1A therapy, prescribing and/or administrating an anti-TL1A therapy to the patient based on the patient's expression levels of biomarker genes.

Abstract: Markers of caloric restriction (CR) can be identified in a selected tissue by exposing an animal to CR conditions and selecting one or more genes differentially expressed in response to CR conditions in multiple subject groups. A candidate compound can be screened for likely ability to mimic the effects of CR when administered to an animal by comparing the tissue levels of expression products of the genes in animals treated with the candidate compound to those of animals subjected to CR.

Abstract: Disclosed are methods of diagnosis of a pathogen-associated disease. These methods comprise: providing a biological sample from a human subject; determining presence, absence and/or quantity of a bacterial pathogen, a viral pathogen, or a combination thereof, by a pathogen culture, a serum antibody detection test, a pathogen antigen detection test, a pathogen DNA and/or RNA detection test, or a combination thereof; determining in the sample, expression levels of at least one endogenous gene in which aberrant expression levels are associated with infection with a pathogen, by a microarray hybridization assay, RNA-seq assay, polymerase chain reaction assay, a LAMP assay, a ligase chain reaction assay, a Southern, Northern, or Western blot assay, an ELISA or a combination thereof. The subject can be diagnosed with the disease if the subject comprises the pathogen and an aberrant level of expression of an endogenous gene.

Abstract: The present invention provides a method for determining whether an individual with relapsing-remitting multiple sclerosis will suffer a relapse or respond to treatment for MS. A ratio of mRNA levels of Response Gene to Complement-32, FasL or IL-21 to L13 determined for an individual provides a normalized level which is compared to a cut-off value. A normalized level of Response Gene to Complement-32 greater than 2.52, a normalized level of FasL greater than 85.4 and a normalized level of IL-21 less than 11.9, respectively, indicates the individual will have or is having a relapse of multiple sclerosis. Also provided are methods for determining whether an individual will respond positively or is responding positively to glatiramer treatment and whether the individual is in a period of stable disease or is not at risk for relapse of multiple sclerosis by comparing normalized levels with the respective cut-off levels.

Abstract: Disclosed are gene signatures that may be used to predict the recurrence of colorectal cancer in a human patient. These signatures can be used to determine when to treat a patient with post-operative adjuvant chemotherapy, i.e., when a high risk of colorectal cancer recurrence is predicted.

Abstract: Provided are kits comprising biomarkers for detection of early stages (I and II) of colorectal cancer and methods for diagnosis and treatment of colorectal cancer at early stages (I and II).

Abstract: Disclosed are a novel method of detecting cancer, a method of screening inhibitors and anticancer agents that target cancer-related molecules, RHOA polypeptide having mutation and a polynucleotide encoding the polypeptide as a therapeutic agent for cancer, and a method of detecting cancer using the polypeptide or polynucleotide. Also disclosed are a vector and a host cell comprising the polynucleotide, a method of screening therapeutic agents for cancer comprising the polypeptide and/or the polynucleotide, and a therapeutic agent for cancer comprising siRNA having a silencing effect on RHOA mutant.

Abstract: This invention concerns the discovery of two distinct PCA3 mRNA sequences. One of these sequences corresponds to a short PCA3 mRNA molecule whereas the other PCA3 RNA molecule is longer as it comprises an additional sequence between exon 3 and exon 4a. The short RNA is associated with prostate cancer whereas the long RNA sequence is associated with a non-malignant state of the prostate. Based on the differential expression levels of these two PCA3 RNA sequences, protocols for the diagnosis of prostate disease are provided. The invention also relates to therapeutic approaches to prostate cancer.

Abstract: A method for detecting cancer by determining ratios of alternatively spliced Lamin A/C gene mRNAs in tissue samples, especially an increased ratio of Lamin C to Lamin A mRNAs. Therapeutic for subjects having a tumor or cancer characterized by an elevated ratio of Lamin C to Lamin A mRNA or protein.

Abstract: Described is method of treating a patient or exempting a patient from further treatment subsequent to tumor removal such as surgery. The method involves performing a highly sensitive real-time PCR for specific detection of transcripts for more than one MAGE gene and a reference gene such as porphobilinogen desaminase (PBGD), glyceraldehyd-3-phosphate dehydrogenase (GAPDH), beta-2-microglobin or beta-actin in a tissue sample of a tumor patient.

Abstract: In one aspect, there is provided a method for predicting responsiveness of a subject to a poly-(ADP-ribose)-polymerase (PARP) inhibitor for treating acute myeloid leukaemia (AML), the method comprising determining whether a chromosomal abnormality selected from t(8;21), t(15;17), t(16;16) and inv(16) is present in a sample obtained from the subject; wherein the presence of the chromosomal abnormality is indicative of responsiveness of the subject to the PARP inhibitor for treating AML.

Abstract: TERT promoter mutations occur in both papillary and flat lesion bladder cancers, are the most frequent genetic alterations identified to date in noninvasive precursor lesions of the bladder, are detectable in urine, and appear to be strongly associated with bladder cancer recurrence. The TERT promoter mutations are useful urinary biomarker for both the early detection and monitoring of bladder neoplasia.

Abstract: Disclosed are methods for determining the methylation status of the promoter region of the TWIST1 gene in genomic DNA from bladder cells, including bladder cells present in a urine sample. Also disclosed are kits for performing the disclosed methods, such as kits for determining the methylation status of the promoter region of the TWIST1 gene comprising at least one primer pair for determining the methylation status of TWIST1. The kits may contain means for processing a urine sample.

Abstract: Methods and systems for quantification of a target nucleic acid in a sample are provided. The method includes forming a plurality of discrete sample portions. Each of the plurality of discrete sample portions comprising a portion of the sample, and a reaction mixture. The method further includes amplifying the plurality of discrete sample portions to form a plurality of discrete processed sample portions. At least one discrete processed sample portion containing nucleic acid amplification reaction products. Fluorescence signals are detected from the at least one of the plurality of discrete processed sample portions to determine a presence of the at least one target nucleic acid. The method also includes determining the respective volumes of the plurality of the plurality of discrete processed sample portions, and estimating the number of copies-per-unit-volume of the at least one target nucleic acid in the sample.

Abstract: Compositions and methods associated with recurrent MIPOL1-ETV1 genetic rearrangements that are useful for cancer diagnosis and therapy are disclosed.

Abstract: Nucleic acid sequencing methods and related products are disclosed. Methods for sequencing a target nucleic acid comprise providing a daughter strand produced by a template-directed synthesis, the daughter strand comprising a plurality of subunits coupled in a sequence corresponding to a contiguous nucleotide sequence of all or a portion of the target nucleic acid, wherein the individual subunits comprise a tether, at least one probe or nucleobase residue, and at least one selectively cleavable bond. The selectively cleavable bond(s) is/are cleaved to yield an Xpandomer of a length longer than the plurality of the subunits of the daughter strand, the Xpandomer comprising the tethers and reporter elements for parsing genetic information in a sequence corresponding to the contiguous nucleotide sequence of all or a portion of the target nucleic acid. Reporter elements of the Xpandomer are then detected.

Abstract: The disclosed invention is related to methods, compositions, kits and isolated nucleic acid sequences for targeting Herpes Simplex Virus (HSV) nucleic acid (eg. HSV-1 and/or HSV-2 nucleic acid). Compositions include amplification oligomers, detection probe oligomers and/or target capture oligomers. Kits and methods comprise at least one of these oligomers.

Abstract: Probes for the detection of oral oncogenic human papillomavirus (HPV) are described. The probe includes a polynucleotide having at least 90% sequence identity to a polynucleotide complementary to a microRNA that has altered expression in response to oncogenic HPV infection. A method for detecting oncogenic HPV in a subject is also described. The method comprises the steps of (A) providing a sample from a subject; (B) measuring the expression level of a microRNA having altered expression in response to oncogenic HPV infection using a probe; and (C) determining that the subject is infected by an oncogenic HPV if the expression level is increased or decreased in comparison with a control.

Abstract: The invention relates to a process for the removal of contaminants from a liquor, the process comprising: introducing a metal or ammonium hydroxide into the liquor; introducing the liquor into a reaction vessel; bubbling a carbon dioxide gas comprising at least 25% carbon dioxide through the liquor within the reaction vessel; and separating the precipitate formed by the carbonatation of the metal hydroxide from the liquor, the precipitate comprising at least some of the contaminants from the liquor; wherein, on average, the liquor is resident within the reaction vessel for a period of no more than about 60 minutes. The invention also relates to a process for the removal of contaminants from a liquor, the process comprising: introducing a metal or ammonium hydroxide into the liquor and bubbling a carbon dioxide gas comprising at least 25% carbon dioxide through the liquor to form a precipitate by carbonatation in a period of no more than about 60 minutes.

Abstract: The present invention discloses a method for isolating and purifying anhydrogalactose. Using the method for isolating and purifying anhydrogalactose including the steps of preparing a sugar mixture containing anhydrogalactose produced through chemical synthesis and hydrolysis; and isolating anhydrogalactose by performing recycling preparative liquid chromatography (recycling-prep-LC) with the sugar mixture, highly pure anhydrogalactose can be efficiently isolated and purified in large amounts.

Abstract: A method for operating a blast furnace that increases combustion temperature and reduces fuel consumption rate is provided. The method includes injecting hot air into the blast furnace from a tuyere. A solid reduction agent and at least one of a flammable reduction agent and a combustion-supporting gas are injected into the blast furnace, with the hot air, from the tuyere and through a lance. The solid reduction agent contains 65 mass % or less of particles whose particle diameter is greater than or equal to 75 ?m. The method facilitates efficient mixing, accelerates the reaction between the pulverized coal and the combustion-supporting gas, and increases the temperature of the pulverized coal. Therefore, the combustion speed of the pulverized coal is increased, which increases the combustion temperature and reduces the reduction agent ratio.

Abstract: The present document describes a smelting apparatus for smelting metallic ore. The smelting apparatus comprises a furnace having a continuous curved wall and end walls defining a longitudinal volume having a longitudinal axis in a horizontal direction. The continuous curved wall has a lowermost area. The longitudinal volume is divided in at least three longitudinal layers comprising a top layer within which gasified fuel is combusted for creating a hot gas composition at a temperature sufficient to release, from the metallic ore, at least molten metal and slag, a lowermost layer at the lowermost area for holding molten metal, and a mid layer above the lowermost layer in which the slag accumulates.

Abstract: A method for producing high carbon content metallic iron using coke oven gas, including: dividing a top gas stream from a direct reduction shaft furnace into a first top gas stream and a second top gas stream; mixing the first top gas stream with a coke oven gas stream from a coke oven gas source and processing at least a portion of a resulting combined coke oven gas stream in a carbon dioxide separation unit to form a synthesis gas-rich gas stream and a carbon-dioxide rich gas stream; delivering the synthesis gas-rich gas stream to the direct reduction shaft furnace as bustle gas; using the carbon-dioxide rich gas stream as fuel gas in one or more heating units; and delivering the second top gas stream to the direct reduction shaft furnace as bustle gas.

Abstract: There is disclosed a method of manufacturing a hot-rolled steel product, such as a hot-rolled steel strip or plate product, wherein the microstructure of the steel product is martensitic, having Brinell hardness of at least 450 HBW. The method comprises the following steps in given sequence: a step of providing a steel slab containing, in terms of weight percentages, C: 0.25-0.45%, Si: 0.01-1.5%, Mn: 0.4-3.0%, Ni: 0.5-4.0%, Al: 0.01-1.2%, Cr: less than 2.0%, Mo: less than 1.0%, Cu: less than 1.5%, V: less than 0.5%, Nb: less than 0.2%, Ti: less than 0.2%, B: less than 0.01%, Ca: less than 0.01%, the balance being iron, residual contents and unavoidable impurities; a heating step of heating the steel slab to a temperature Theat in the range 950-1350° C.; a temperature equalizing step; a hot-rolling step in a temperature range of Ar3 to 1300° C. to obtain a hot-rolled steel material; and a step of direct quenching the hot-rolled steel material from the hot-rolling heat to a temperature of less than Ms.

Abstract: In a heating step (S1) of inductively heating a steel ring-shaped member (R) to a target temperature, a plurality of ring-shaped members (R) retained by a retaining unit (3) coaxially with each other are caused to sequentially pass through an opposing region of a first heating coil (21) having a constant output, and through an opposing region of a second heating coil (22) having a variable output and being arranged coaxially with the first heating coil (21) on an exit side of the first heating coil (21). Thus, the plurality of ring-shaped members (R) are sequentially and inductively heated to the target temperature.

Abstract: A heat treatment method for a steel material according to the present invention includes: a first step of forming austenite by heating the steel material to a temperature equal to or higher than an A1 point; a second step of cooling the steel material heated in the first step, while keeping the steel material at a temperature higher than an Ms point, thereby causing the austenite of the steel material to be transformed into ferrite, pearlite, or bainite; and a third step of cooling the steel material to a temperature equal to or lower than the Ms point after the second step. According to the present invention, it is possible to provide a heat treatment method for a steel material which is capable of shortening a heat treatment time while suppressing the formation of martensite.

Abstract: A method of manufacturing a hot press formed part by hot pressing a coated steel sheet that is obtained by forming a Zn-based coating layer on a surface of a steel sheet includes: heating the coated steel sheet to a temperature range of 750° C. or more and 1000° C. or less; cooling a surface of the coated steel sheet; and hot press forming the coated steel sheet under a condition that a surface temperature of the coated steel sheet is 400° C. or less and an average temperature of the coated steel sheet is 500° C. or more or a temperature of a center position of the coated steel sheet in a thickness direction is 530° C. or more.

Abstract: A non-quenched and tempered steel and manufacturing process thereof. The process alters the cooling mode before finish rolling in previous production of non-quenched and tempered steel; the process at least has a cooling step after the finish rolling step; the process utilizes alternate intense cooling and moderate cooling. The intense cooling can ensure the surface temperature of the steel to decrease rapidly; and the moderate cooling allows the core temperature of the steel to dissipate gradually to the surface; a further intense cooling is carried out to allow rapid heat dissipation. The intense cooling and the moderate cooling can be carried out alternately several times accordingly. A water cooling mode combining intense cooling and moderate cooling allows the core temperature and the surface temperature of the steel to become the same within a short time, the uniformity of the mechanical properties of the steel.

Abstract: A process for producing a non quenched and tempered steel. The process comprises a cooling step at least after a finish rolling step. In said cooling step, an intense cooling and a moderate cooling are carried out alternately to allow the steel to undergo at least two stages of water cooling, so that the core temperature and the surface temperature of the steel become the same within a specified time, and thus ensures the uniformity of the mechanical properties of the steel and improves the production efficiency.

Abstract: A non quenched and tempered steel and manufacturing process thereof. The process comprises a cooling step after the finish rolling step; and the process utilizes alternate intense cooling and moderate cooling. The intense cooling can ensure the surface temperature of the steel to decrease rapidly; and the moderate cooling allows the core temperature of the steel to dissipate gradually to the surface; a further intense cooling is carried out to allow rapid heat dissipation. The intense cooling and the moderate cooling can be carried out alternately several times according to practical requirement. A water cooling mode combining intense cooling and moderate cooling allows the core temperature and the surface temperature of the steel to become the same within a short time, and thus ensures the uniformity of the mechanical properties of the steel and improves the production efficiency.

Abstract: A high-strength steel sheet of the present invention is a steel sheet satisfying a predetermined component composition. A metal structure of the steel sheet is composed of polygonal ferrite, high-temperature region generated bainite, low-temperature region generated bainite and retained austenite each having a predetermined area percent, and a distribution using each average IQ of predetermined crystal grains determined by electron backscatter diffraction satisfies Equations (1) and (2) below. According to the present invention, a high-strength steel sheet having excellent ductility and low-temperature toughness can be realized even at a tensile strength of 780 MPa or more. (IQave?IQmin)/(IQmax?IQmin)?0.40??(1) (?IQ)/(IQmax?IQmin)?0.

Abstract: A method comprises heating an aqueous solution of colloidal silver particles. A soluble noble metal halide salt is added to the aqueous solution which undergoes a redox reaction on a surface of the silver particles to form noble metal/silver halide SPs, noble metal halide/silver halide SPs or noble metal oxide/silver halide SPs on the surface of the silver particles. The heat is maintained for a predetermined time to consume the silver particles and release the noble metal/silver halide SPs, the noble metal halide/silver halide SPs or the noble metal oxide/silver halide SPs into the aqueous solution. The aqueous solution is cooled. The noble metal/silver halide SPs, the noble metal halide/silver halide SPs or noble metal oxide/silver halide SPs are separated from the aqueous solution. The method optionally includes adding a soluble halide salt to the aqueous solution.

Abstract: A method of recovering gold from carbon fines in a resin-in-leach process wherein the fines are contacted with an ion exchange resin, in the presence of a suitable lixiviant, to load gold onto the resin, eluting the gold from the resin using a suitable eluent and recovering the gold from a resulting eluate.