Abstract: Disclosed is an aqueous composition having viscosity from 1 to 100 cP at 20° C., said composition comprising: (i) an oligodynamic metal or ions thereof; (ii) a chelating agent; and, (iii) free alkali less than 1 wt %, wherein said composition comprises 0.01 wt % to 2 wt % of a salt of an organic acid; pH of the composition is from 9 to 12 and molar ratio of said oligodynamic metal to said chelating agent is 1:0.25 to 1:10. The composition provides a robust solution for technical problems of discolouration and in stability.

Abstract: The present invention relates to methods for modifying a non-cellulosic textile material comprising treating the non-cellulosic textile material with a composition comprising a xyloglucan endotransglycosylase and (a) a polymeric xyloglucan and a functionalized xyloglucan oligomer comprising a chemical group; (b) a polymeric xyloglucan functionalized with a chemical group and a functionalized xyloglucan oligomer comprising a chemical group; (c) a polymeric xyloglucan functionalized with a chemical group and a xyloglucan oligomer; (d) a polymeric xyloglucan and a xyloglucan oligomer; (e) a polymeric xyloglucan functionalized with a chemical group; (f) a polymeric xyloglucan; (g) a functionalized xyloglucan oligomer comprising a chemical group; or (h) a xyloglucan oligomer, under conditions leading to a modified non-cellulosic textile material, where the modified non-cellulosic textile material possesses a textile improvement compared to the unmodified non-cellulosic textile material.

Abstract: The present invention relates to a zero-phosphate machine dish wash composition in unit dose format comprising non-phosphate builder, alkali percarbonate, a manganese bleach catalyst in relatively low amounts and one or more polycarboxylate polymers. The builder comprises one or more from methylglycine-N,N-diacetic acid and/or one or more salts thereof, citric acid and/or one or more salts thereof, and glutamic acid-N,N-diacetic acid and/or one or more salts thereof. The polycarboxylate polymers have a weight average molecular weight of between 1000 and 100,000, the polymers comprising at least 20 mol-% of acrylate monomers and from 0 to 40 mol-% of maleate monomers. The composition provides improved tea stain removal upon use.

Abstract: A first aspect relates to a particle including: (a) at least one bleaching agent including an organic peroxyacid; (b) at least one component selected from: (i) saccharides and polysaccharides, and derivatives thereof; (ii) inorganic fillers selected from the group consisting of talcs, micas, zeolites, silicates, metal oxides and clays; and (iii) linear, branched and cross-linked polymers and copolymers; (c) at least one pH reducing agent; and (d) at least one chelating agent. Further aspects relate to a process for preparing the particle, a composition or a dough including the particle, and use of the particle in a cleaning composition, a dental care composition, a hair dyeing composition or decolouriser, an antimicrobial composition or a bleach composition.

Abstract: The present invention provides a treatment composition comprising a hydrophobic dye.

Abstract: Disclosed is a cleansing composition having pH of at least 9, said composition comprising: (i) 20 to 85 wt % anionic surfactant; and, (ii) a silver(I) compound having silver ion solubility (in water at 25° C.) of at least 1×10?4 mol/L, at a level equivalent to silver content of 0.01 to 100 ppm, wherein the free alkali content of said composition is less than 0.01%. The composition is a robust and improved cleansing composition with a stable colour.

Abstract: Fluid fabric enhancer compositions comprising microcapsules having a cationic, nonionic and/or anionic coating, a formaldehyde source that can comprise components of said microcapsules and a formaldehyde scavenger, as well as processes for making and using such fluid fabric enhancer compositions. Such fluid fabric enhancer compositions contain a formaldehyde scavenging system that provides more consistent formaldehyde scavenging over time.

Abstract: The present invention relates to a cosmetic composition, particularly indicated for skin cleaning, which comprises at least one plant oil comprising 40% to 60% of fatty chain containing 12 carbons, C12, such chain may be saturated or unsaturated; at least one saponifying agent; and aqueous portion. The invention also relates to a process for preparing said cosmetic composition, as well as the use thereof.

Abstract: One- or multi-phase non-solid concentrated products, which are capable of producing or maintaining a Winsor type 2 microemulsion system upon dilution in a short liquor technology washing machine, show improved performance for fat and oil-containing stains when used in novel methods in which various Winsor types are passed through starting from Winsor type 2.

Abstract: The present application relates to high efficiency particles and compositions, such as consumer products, comprising such high efficiency particles as well as processes for making and using such high efficiency particles and compositions comprising such high efficiency particles. Such high efficiency particles and compositions provide enhanced benefit agent delivery to a situs that is treated with such high efficiency particles and compositions.

Abstract: A process for making a single or multi-compartment water-soluble cleaning pouch, the pouch comprising an enveloping material and a detergent composition, the detergent composition comprising a cohesive powder and having a low pH, the process comprising the steps of: i) making a first open compartment and filling the first open compartment with a homogeneous free-flowing powder comprising the cohesive powder in the form of a secondary particle wherein at least 80% of the homogeneous free-flowing powder has a weight average particle size of from about 250 to about 850 ?m with less than about 10% by weight of the particles below about 150 ?m and less than about 5% by weight of the particles above about 1180 ?m and wherein the powder has a cake strength of less than about 20 N; ii) optionally making a second open compartment and filing the second open compartment with a liquid; and iii) closing the open compartment(s) to make a pouch.

Abstract: A process for making a multi-compartment water-soluble automatic dishwashing cleaning pouch, the pouch comprising an enveloping material and a detergent composition, the detergent composition has a low pH and comprises a lime soap dispersing agent, the process comprising the steps of: i) making a first open compartment and filling the first open compartment with a liquid composition having a viscosity of from about 1 to about 500 mPa s at 23° C. wherein the liquid composition comprises more than 20% by weight of the liquid of an aqueous lime soap dispersing agent and has a pH of less than 10; ii) making a second open compartment and filing the second open compartment with a powder; and iii) closing the open compartments to make a pouch.

Abstract: A brewing apparatus for small scale brewing of fermented beverages such as beer and cider and a method of brewing using the apparatus is described. The brewing apparatus includes a single pressurizable vessel in which all the steps of the brewing process can be carried out, including fermentation, carbonation (naturally during fermentation), sediment removal, maturation and draw off. The brewing apparatus includes a safety pressure release system to relieve pressure from within the brewing vessel if the pressure differential between the interior and the exterior of the brewing vessel exceeds a predetermined pressure. The brewing apparatus is configured so it can be easily transported from one location to another. In one embodiment, the brewing apparatus has a scalable lid or hatch which includes all of the necessary components and connections located therein.

Abstract: The present invention relates to the field of production of a beverage, especially cider, by fermentation of apple juice and/or pear juice. Specifically, the invention relates to a method of preparing a beverage with enhanced levels of desirable fermentation-derived flavor compounds comprising a step of fermentation of apple juice and/or pear juice with at least one Pichia kluyveri yeast strain, a beverage obtainable by the method and a cider comprising a high concentration of the flavor compound, hexyl acetate.

Abstract: A bioprocessing system for protein manufacturing is provided that is compact, integrated and suited for on-demand production and delivery of therapeutic proteins to patients. The system can also be used for efficient on-demand production of any type of protein.

Abstract: An object is to provide a cell culture vessel capable of improving culture operability. The present invention relates to a cell culture vessel comprising a bottom and a side wall, wherein at least one microwell for containing a cell is disposed on the bottom, the microwell has a bottom surface, a side surface, and an opening, a convex-concave structure is formed on the side surface of the microwell, and the side surface of the microwell is subjected to hydrophilization treatment.

Abstract: According to an aspect of some embodiments, an apparatus for isolation and cytometric analysis of cells from a liquid medium is provided. The apparatus comprises a cell reservoir, for receiving a liquid medium containing a suspension of cells. The apparatus further comprises a cell cage array between the cell reservoir and an enclosed reservoir, each cell cage comprising a large opening adjacent to the first cell reservoir, a cell cage, and one or more small opening adjacent to the enclosed reservoir. A connected liquid medium pump may move the suspended cells into cell cages by flowing the liquid medium from the cell reservoir to the enclosed reservoir. The apparatus further comprises a mechanical element which when actuated, pushes the cell cage array against a gel layer, forming a contiguous barrier to isolate each cell in a cell cage, and allowing cytometric analysis of isolated cells.

Abstract: A culture container includes a gel section constituted by a material containing a stimulus-responsive gel which expands or contracts in response to a stimulus by a given substance in at least a portion of a part coming into contact with a culture solution.

Abstract: Provided herein is a continuous cell perfusion model system that provides useful pharmacokinetic and pharmacodynamic information on the application of new drugs or combinations of various agents in vitro to human cancer cell lines. Also provided are methods of using this system to individualize cancer treatment.

Abstract: Biomass feedstocks (e.g., plant biomass, animal biomass, and municipal waste biomass) are processed to produce useful products, such as fuels. For example, systems are described that can convert feedstock materials to a sugar solution, which can then be fermented to produce ethanol. Biomass feedstock is dispersed in a liquid medium and then saccharified.

Abstract: A biogas stirring device for an anaerobic tank includes: a biogas compressor, a biogas distributer, and gas inlet valves; wherein the biogas distributer includes a main pipe and a plurality of sub-pipes communicating with the main pipe; wherein a tank wall of the anaerobic tank has a plurality of installing holes corresponding to an amount of the sub-pipes; each of the installing holes is provided with one of the gas inlet valves, and an inlet end of each of the gas inlet valves communicates with one of the sub-pipes; an outlet end of each of the gas inlet valves communicates with an inside area of the anaerobic tank. Advantages of the present invention are as follows: the present invention effectively solves blockage of the conventional exhaust pipes; the present invention is simple in structure, low in cost, convenient in installation and easy in maintenance.

Abstract: The present invention discloses a dual chamber selectively permeable cell culture device, wherein, the said device includes: at least two cell culture chambers: the first cell culture chamber and the second cell culture chamber; wherein, the said first cell culture chamber is configured to culture supporting cells, which are able to secrete supporting materials, and the said second cell culture chamber is applied to take the supporting materials and culture target cells; the said first cell culture chamber connects to the second cell culture chamber through a semi-permeable membrane; each of the said first and second cell culture chamber has a liquid inlet and an inoculation channel. It is possible to reduce the cost for target cells culture effectively, while the structure of the said device is simple and easy to achieve, having a good market prospect.

Abstract: The present invention is directed to compositions, tools, methods and devices to culture microorganisms and, in particular, to compositions, tools, methods and devices for the detection of microorganisms in biological samples.

Abstract: By deleting a small area from an initial boundary image, a provisional boundary image is obtained (Step S211). A thinning processing is performed on the provisional boundary image to shape the image, and a thickening processing is performed on the thinned image to obtain a mask image (Steps S212 to S214). By masking the initial boundary image with the mask image, an updated provisional boundary image is obtained (Step S215). In the updated provisional boundary image, end points adjacent to each other are connected (Step S216). By repeating Steps S212 to S216, a broken boundary is repaired. As a result, even if a boundary of a cell colony is blurred, an area of the cell colony can be specified easily and efficiently.

Abstract: The present invention relates to an apparatus that uses shadow images of cells to continuously measure cell activity at a high processing rate in order to provide cell activity and cell number results. According to one embodiment of the present invention, instead of a highly experienced examiner or technician using a microscope, ELISA reader, etc. having to collect various cell activity measurements and cell numbers, the collection of said information can be automated so as to reduce cost and largely reduce errors in measurements through the development of computer software coupled with hardware using low cost and compact optoelectronic components and simple image processing techniques.

Abstract: Systems and methods for automatically controlling conditions of a process are disclosed. In one example, a controller is programmed with a sequence of steps and parameters required to carry out a bioreactor process. A sensor system interacts with the bioreactor to receive information related to a condition of the bioreactor and/or receive a sample from the bioreactor, which it analyzes. The sensor system sends data signals related to the information and/or the sample to a controller, which determines a control signal based on the received information. The controller sends the control signal to the sensor system which, based on the control signal, performs an action that affects a condition of the bioreactor or affects the sensor system itself.

Abstract: The present application discloses a system and method for preservation, storage and transport of biological materials by the use of at least one deformable container configured with at least one inlet at one end, an outer surface greater than 80% of the total surface of the cavities (101) of the support (100) and a width and length sufficient for it to be placed in contact with all the heat transfer plates (132) of the support (100). The support (100) has at least two cavities (101) delimited by the heat transfer plates (132), with means for temperature control, side walls (103) and at least one main front door (102). This technology allows improvement of the heat transfer necessary for freezing and thawing large volumes of solutions containing biological materials. This technology allows to considerably accelerate the heat transfer, as well as the reproducibility and scalability of the freezing and thawing process while keeping the system very compact.

Abstract: The invention relates to a system and a method for cell separation via a filter, with a powder bag which can be arranged in a recirculation circuit and is partly filled with a pulverulent filter aid and to which a cell solution containing the cells to be separated can be delivered from a bioreactor via a pump arranged in the recirculation circuit, the bioreactor, which has an agitator, being arranged in the recirculation circuit, and, when the recirculation circuit is interrupted, the bioreactor being connectable to the filter.

Abstract: Methods of inducing or increasing lipid and protein production in microalgae are disclosed. Methods of enhancing microalgae biomass are also disclosed. The methods may comprise inoculating a culture media with microalgae and propagating the microalgae under heterotrophic growth conditions. The heterotrophic growth conditions may comprise inhibiting exposure of the inoculated culture media to light. The methods may further comprise delivering oxygen into the inoculated culture media. Additionally, lipids, proteins, and other cellular components may be isolated and purified from microalgae cultivated under the disclosed methods.

Abstract: A method for cultivating antrodia cinnamomea includes transplanting bacterial strains of antrodia cinnamomea into a petri dish receiving culture ingredients. The bacterial strains of antrodia cinnamomea are transferred into a culture container after mycelia of the bacterial strains of antrodia cinnamomea have grown to a predetermined area. The culture container receives a culture fluid. A formula composition of a culture medium for cultivating antrodia cinnamomea is added into the culture container after the mycelia of the bacterial strains of antrodia cinnamomea have grown in the culture container. The formula composition of the culture medium for cultivating antrodia cinnamomea includes growth basal ingredients and cultivating basal ingredients. The growth basal ingredients includes hydroxyhopanone.

Abstract: The present invention relates to a method for obtaining a strain capable of efficiently propagating in a low nutritive potential medium, capable of metabolising pentoses and of resisting fermentation inhibitors, comprising the following steps; a) growth of a strain of recombinant yeast with a strain of wild yeast lacking any impairments, the recombinant yeast strain comprising at least one copy of an exogenous gene of xylose isomerase and at least one additional copy of a gene of D-xylulokinase included in the genome and linked to a single sexual characteristic of the strain, b) at least two cycles of genome shuffling by sporulation and or random hybridization, c) selection of the population obtained in step b) according to a suitability criterion of the strains to metabolize xylose, d) selection of the population obtained in step c) according to a suitability criterion of the strains to grow in a Pref type medium, a medium with low nutritive value.

Abstract: The present invention discloses a culture medium for hematopoietic stem cells and the applications thereof as well as the stem cells culture method, which adopts amino acids, vitamins, salts, lipids, protein polypeptides and cytokines, together with one or several of trace elements, small organic molecules, hematopoietic stem cell proliferation agents, plant extracts and animal tissue cell extracts, to compose the hematopoietic stem cell culture medium, which can quickly proliferate different species of CD34+ cells, and improve both the proliferation speed and telomere length of CD34+ cells significantly; also, hematopoietic stem cells cultured in the hematopoietic stem cell culture medium described in the present invention may keep their cell functions as well, achieve the functions include but not limited to anti-tumor, anti-aging, and extending lives, thus having a pretty good application prospect.

Abstract: Provided are: a cell tray provided with a concave part for supporting a cell aggregate and a hole formed on the bottom of the concave part; and a device for producing a cell structure, said device being provided with the cell tray and a puncture part passing through the cell tray and the cell aggregate, characterized in that the puncture part passes through the cell aggregate supported by the concave part until the tip thereof intrudes into the hole.

Abstract: A composition comprising a plurality of cell aggregates for use in the production of engineered organotypic tissue by organ printing. A method of making a plurality of cell aggregates comprises centrifuging a cell suspension to form a pellet, extruding the pellet through an orifice, and cutting the extruded pellet into pieces. Apparatus for making cell aggregates comprises an extrusion system and a cutting system. In a method of organ printing, a plurality of cell aggregates are embedded in a polymeric or gel matrix and allowed to fuse to form a desired three-dimensional tissue structure. An intermediate product comprises at least one layer of matrix and a plurality of cell aggregates embedded therein in a predetermined pattern. Modeling methods predict the structural evolution of fusing cell aggregates for combinations of cell type, matrix, and embedding patterns to enable selection of organ printing processes parameters for use in producing an engineered tissue having a desired three-dimensional structure.

Abstract: The invention relates to a large scale process for the in vitro production of a cell culture of pomegranate cells, to a product prepared from such cells and to a composition comprising a complex of pholyphenols, including punicalagin, 1,2,3,4,6-pentagalloyl glucose (PGG) or both.

Abstract: The present invention provides cell populations that are enriched for mesendoderm and mesoderm, and cell populations that are enriched for endoderm. In accordance with the present invention, a selectable marker gene has been recombinantly targeted to the brachyury locus to allow the isolation and characterization of cell populations that comprise brachyury positive mesendoderm and mesoderm cells. The cell populations of the invention are useful for generating cells for cell replacement therapy.

Abstract: The present disclosure relates to methods for producing dopaminergic cells and evaluating their functionality. When pluripotent human embryonic stem cells are cultured on plates coated with laminin-111, laminin-121, laminin-521, laminin-421, or laminin-511 in cell culture medium containing a GSK3 inhibitor and a TGF-? inhibitor as well as timely administered fibroblast growth factor, desired neural cells are produced at far higher rates. Useful cell culture kits for producing such dopaminergic cells are also described herein, as are methods of using such cells for stem cell therapy.

Abstract: Pluripotent cells that are immunopositive for both the neural progenitor marker nestin and a pluripotent cell marker are provided. The cells exhibit rapid doubling times and can be maintained in vitro for extended periods. Also provided are cell cultures containing the pluripotent cells, a method of transplanting human pluripotent cells to a host, and a method of reducing seizure activity in a subject. These pluripotent cells, when transplanted into the ventricle of a host animal, migrate to the site of damage and adopt a suitably corrective phenotype, resulting in both structural and functional restoration.

Abstract: Provided are a system and methods for selectively inducing expansion of a population of T cells in the absence of exogenous growth factors, such as lymphokines, and accessory cells for research purposes. The cell based expansion system and methods permit the long-term growth of CTLs, preferably human CTLs. In addition, T cell proliferation can be induced without the need for antigen, thus providing an expanded T cell population that is polyclonal with respect to antigen reactivity. Further provided are methods for using the system and methods to screen and identify antigens related to specific diseases or conditions, tumors, autoimmune disorders, or an infectious disease or pathogen, and to identify target molecule for research purposes, or for developing a vaccine based thereon.

Abstract: Disclosed herein are modified primary human T cells and populations thereof comprising a genome in which the CTLA4, PD1, TCRA, TCRB, and/or B2M genes have been edited to generate an off-the-shelf universal CAR T cell from allogeneic healthy donors that can be administered to any patient while reducing or eliminating the risk of immune rejection or graft versus host disease, and which are not prone to T cell inhibition, and methods for allogeneic administration of such cells to reduce the likelihood that the cells will trigger a host immune response when the cells are administered to a subject in need of such cells.

Abstract: The invention relates to methods for generating viral-free cells using nucleases for use in transplantation. The nucleases may be CRISPR/Cas9 complexes with guided RNA to target and inactivate viral genomes within cells. The nucleases degrade or destroy the viruses within the cells prior to transplantation.

Abstract: The present invention relates to an immortalized cell, an immortalized cell line comprising said immortalized cell, a cell culture comprising the immortalized cell or cell line, and a method for the production of an immortalized cell.

Abstract: Provided in the present invention are odontogenic stem cells and a use of genetically modified odontogenic stem cells for treating periodontal disease, repairing defects in periodontal bone tissues or soft tissues and/or promoting the regeneration of periodontal tissues, or in products for treating acute and chronic bone tissue injuries (e.g. bone fracture) or bone tissue defects. Also provided in the present invention is a composition comprising odontogenic stem cells and/or genetically modified odontogenic stem cells, wherein an exogenous hepatocyte growth factor gene is introduced into odontogenic stem cells through an adenovirus or adeno-associated virus vector to obtain the genetically modified odontogenic stem cells.

Abstract: Provided is a protein coded by a gene related to cell totipotency and a transcriptional activation domain of a mammalian YAP protein or a fusion protein of a segment with a transcriptional control activity, a coding nucleotide sequence, an expression vector and a composition thereof, as well as a method for inducing the pluripotent stem cells by using the fusion protein.

Abstract: Engineered animal skin, hide, and leather comprising a plurality of layers of collagen formed by cultured animal collagen-producing (e.g., skin) cells. Layers may be formed by elongate multicellular bodies comprising a plurality of cultured animal cells that are adhered and/or cohered to one another; wherein the elongate multicellular bodies are arranged to form a substantially planar layer for use in formation of engineered animal skin, hide, and leather. Further described herein are methods of forming engineered animal skin, hide, and leather utilizing said layers of animal collagen-producing cells.

Abstract: A method of screening a microorganisim having a mutant lactate dehydrogenase with increased activity, a mutant lactate dehydrogenase polypeptide, a polynucleotide and vector encoding same, and a microorganism that expresses the lactate dehydrogenase mutant, and a method of producing lactate using the microorganism.

Abstract: The disclosure relates to acyl-ACP reductase (AAR) enzyme variants that result in improved fatty aldehyde and fatty alcohol production when expressed in recombinant host cells. The disclosure further relates to methods of making and using such AAR variants for the production of fatty alcohol compositions having particular characteristics.

Abstract: The present invention provides a pipecolic acid 4-hydroxylase protein exemplified by the following (A), (B), and (C), having activity to react with L-pipecolic acid in the presence of 2-oxoglutaric acid and iron(II) ions to produce trans-4-hydroxy-L-pipecolic acid, and a method for producing 4-hydroxy amino acid, which method comprises reacting the pipecolic acid 4-hydroxylase protein, cells containing the protein, a treated product of the cells, and/or a culture liquid obtained by culturing the cells, with ?-amino acid to produce 4-hydroxy amino acid: (A) a polypeptide comprising the amino acid sequence represented by SEQ ID NO:2, 4, 6, 8, 10, 12, 16, or 18; (B) a polypeptide comprising the amino acid sequence represented by SEQ ID NO:2, 4, 6, 8, 10, 12, 16, or 18 except that one or several amino acids are deleted, substituted, and/or added, and having pipecolic acid 4-hydroxylase activity; and (C) a polypeptide having an amino acid sequence that is not less than 80% identical to the amino acid sequence rep

Abstract: The present invention relates to a modified microorganism having, compared to its wild-type, a reduced activity of the enzyme that is encoded by the pykA-gene. The present invention also relates to a method for producing an organic compound and to the use of modified microorganisms.

Abstract: Factors For the Production and Accumulation of Polyunsaturated Fatty Acids (PUFAs) Derived from PUFA Synthases Abstract Disclosed are novel enhancing factor proteins of the PUFA synthase systems, nucleic acid molecules encoding the same, recombinant nucleic acid molecules and recombinant host cells comprising such nucleic acid molecules, genetically modified microorganisms comprising the same, and methods of making and using the same. Also disclosed are genetically modified microorganisms that have been genetically modified to express a PUFA synthase system for the production of PUFAs, wherein the microorganisms have been modified to express the novel enhancing factor proteins of the PUFA synthase system.