Abstract: The present invention relates to simple, low-cost, rapid paper-based diagnostic devices and their methods of use.

Abstract: A system and method for the detection and quantification of biomolecules by measuring a piezoelectric signal is described. The system comprises a plurality of elongate zinc oxide nanowires mounted generally parallel to each another on a semi conductive silicon substrate. The free ends of the nanowires are provided with biomolecules that are capable of associating with complementary biomolecules within a biological or water sample. Following incubation of the system in a sample, the association of molecules of interest with the immobilised biomolecules on the system results in the displacement of the zinc oxide nanowires. The displacement of the nanowires produces a piezoelectric voltage signal that is useful in diagnosing a pathogenic infection or the contamination of a sample.

Abstract: Compositions, methods, and devices for the detection of multiple analytes with a single signal are provided.

Abstract: An object is to provide an immunochromatographic analysis method capable of shortening the developing time without decreasing the detection sensitivity, and also capable of reducing the return of the liquid of a developed component, and a method for detecting a detection target contained in an analyte using an immunochromatographic analysis device including an absorption part composed of glass fiber, wherein the analyte and a labeling substance are developed in a chromatography medium part as a mobile phase in the presence of a nonionic surfactant, and the detection target is detected in a detection part is provided.

Abstract: A method for performing multiplexed bead-based immunoassays using a microfluidic cassette capable of detecting a particle passing in substantially single file through an interrogation zone and generating a Coulter effect signal responsive to a characteristic of the particle. A fluid sample may be prepared by associating antibody-coated beads of different sizes to particles of interest. A first multiplexing option may be based on bead size, in which case the intensity of the Coulter signal is used to sort or characterize the particles. A second multiplexing option may be based on detection of Stokes' shift phenomena, or even simply emission intensity, in which case particles may be characterized responsive to intensity of the signal resulting from detection of radiation. The first and second multiplexing options may be employed together to populate an array of particle characteristics.

Abstract: The apparatus has a containing body with a flat upper functional floor for containing receptacles of reagents, diluents and samples, as well as housings for gel cards and incubators for the same; a lower floor containing receptacles for washing liquids and the collection of waste and cards and for housing the fluid control and electronic control system; a series of longitudinal and transverse guides associated with the upper part of the apparatus, suitable for carrying in suspension the moving heads of the apparatus, the heads being movable on the transverse guides; two heads, respectively for perforation and pipetting and for the transport of gel cards; two centrifuges and a gel card reader; and a folding touch screen providing information and control.

Abstract: Provided are a method and means permitting the simultaneous measurement of the reactive properties of more than 10,000 of antigen-stimulated lymphocytes being held on a chip and the separate determination of the states of individual cells. A microwell array comprises multiple wells and a coating layer on one of the principal surfaces of a base member, the wells being of a size permitting the entry of only a single cell into each well. A coating layer of a substance capable of binding to a substance produced by the cells contained in the wells is present on the principal surface around the wells.

Abstract: A test reagent includes a reactant and a sheet-shaped member. The reactant is in a dry state and specifically reacts with a test substance. The sheet-shaped member holds the reactant and disperses in a solvent solution in which the reactant dissolves.

Abstract: Provided are molecule immobilization patterns and a method for forming the same, thereby reducing noise that may occur while analyzing a signal, being stable even at room temperature, and improving orientation of immobilized materials.

Abstract: The present invention is directed to methods and devices for amending undiluted and partially diluted urine samples in a manner suitable for performing immunoassays for target analytes, for example NGAL. Generally, the urine sample is treated with reagents including at least one of buffer materials, water soluble proteins, urease, and other interferent mitigants. These reagents control the pH of the urine sample in a manner suitable for immuno-binding reactions and ameliorate interferences, particularly during the detection step.

Abstract: The present invention relates to the use of absorbent particles to improve the detection of a signal corresponding to the presence of an analyte in an analysis method on spot(s), in particular when the signal detection takes place in the presence of a liquid phase. The present invention also relates to an analysis method on spot(s) making it possible to improve the detection of a signal corresponding to the presence of an analyte, in the presence of a liquid phase comprising absorbent particles.

Abstract: Methods, compositions, and reaction mixtures are provided for identifying a T cell receptor (TCR) and an epitope peptide that specifically binds the TCR. Methods, compositions, and reaction mixtures are also provided for identifying a plurality of T cell receptors and corresponding epitope peptides that specifically bind the T cell receptors. In some cases, the plurality of T cell receptors and corresponding epitope peptides can be identified in a highly parallel manner.

Abstract: The present disclosure is directed to reagents and methods of using the reagents to detect epitopes of Trypanosoma cruzi.

Abstract: Disclosed herein are diagnostic devices, kits, and methods for the detection of an active Staphylococcus infection in an individual. Utilizing a sample from the individual, antibodies specific for one or more Staphylococcus polypeptides are detected, where the detection of a threshold number of antibodies specific for one or more Staphylococcus polypeptides indicates the presence of an active Staphylococcus infection. Exemplary panels of Staphylococcus polypeptides that can be used with a high degree of specificity and sensitivity are disclosed.

Abstract: This invention relates to high throughput methods of determining a viral titer. The instant invention addresses the need for a more rapid and cost effective method of quantitating infectious viral particles in a sample.

Abstract: MMP-8 activation products are disclosed that have a MMP-8 middle-part activation product having a size between 20-35 kDa. Methods of detecting the MMP-8 activation product or activated MMP-8 fragments in a biological sample derived from a subject are also disclosed. The MMP-8 activation product or activated MMP-8 fragments diagnose diseases based on abnormal or elevated levels of activated MMP-8.

Abstract: Compositions and methods for predicting the presence of a mycobacterial infection in a subject are provided. In some embodiments, the method further comprises assaying the sample to directly detect the presence of the mycobacterial infection if infection is predicted. However, as this is a time-consuming and expensive process, the disclosed methods can be used to predict the presence of the mycobacterium prior to confirmation by direct detection, thereby saving time and money. The disclosed method involves assaying a biological sample from the subject for detection of selenium, wherein the presence of selenium in the sample is an indication of mycobacterium in the sample. Once a mycobacterium is predicated, and optionally confirmed by direct detection, the method can further comprising treating the subject with a therapeutically effective amount of an antibiotic.

Abstract: The invention relates to immunomodulation of cells and the detection and use thereof, for example, in drug screening, including methods, compositions and systems therefor, or in an aspect of healthcare, such as prognosis, diagnosis, an aspect of treatment, monitoring, and the like, and methods, compositions, and systems thereof.

Abstract: The present invention is based on the discovery that three proteins, Cystatin B, Chaperonin 10, and Profilin are present in the urine of patients with bladder cancer, a cancer of epithelial origin. Accordingly, the present invention is directed to methods for prognostic evaluation of cancers of epithelial origin and to methods for facilitating diagnosis of cancers of epithelial origin by monitoring the presence of these markers in biological samples. The invention is also directed to markers for therapeutic efficacy.

Abstract: The present disclosure relates to a panel of a plurality of metabolite species that is useful for the identification or detection of subjects having pancreatic cancer, including methods for identifying such metabolic biomarkers within biological samples. The disclosure also includes a statistical model for predicting the presence of pancreatic cancer in a subject's biofluid by quantifying and comparing positive and negative fold changes in metabolite species' concentration; comparing the subject's metabolite species' concentrations to a predetermined value.

Abstract: Novel antibodies that specifically bind human NOTCH3 are provided. Methods and kits using these antibodies to determine the level of NOTCH3 expression in tumor samples are provided.

Abstract: The present invention is directed to the identification of markers that can be used to determine whether patients with cancer are clinically responsive or non-responsive to a therapeutic regimen prior to treatment. In particular, the present invention is directed to the use of certain combinations of markers, wherein the expression of the markers correlates with responsiveness or non-responsiveness to a therapeutic regimen comprising proteasome inhibition. Thus, by examining the expression levels of individual markers and those comprising a marker set, it is possible to determine whether a therapeutic agent, or combination of agents, will be most likely to reduce the growth rate of tumors in a clinical setting.

Abstract: Method of enriching specific cells from cellular samples are disclosed, comprising contacting in solution a cellular sample with affinity-tagged ligands (ATLs) each comprising a first ligand linked to an affinity tag, wherein the ligand selectively binds a cellular marker of the rare cells and the affinity tag can be selectively captured by a capture moiety, wherein the affinity tags do not comprise a magnetic particle; and flowing the sample through a microfluidic device comprising the capture moiety to selectively retain ATL-bound cells. Methods for enriching circulating tumor cells, and devices for enriching specific cells from cellular samples are also disclosed.

Abstract: Disclosed herein are methods for detecting the presence and/or amount of HER2 protein, HER2 nucleic acid (for example, HER2 genomic DNA), ER protein, and Chromosome 17 centromere DNA in a single sample. Samples stained for HER2 protein, HER2 DNA, ER protein, and Chromosome 17 DNA allow for the identification of various types of cancer cells, for example HER2 protein positive/ER protein positive/HER2 gene positive cells, HER2 protein positive/ER protein negative/HER2 gene positive cells, HER2 protein negative/ER protein positive/HER2 gene positive cells, and HER2 protein negative/ER protein negative/HER2 gene positive cells.

Abstract: Disclosed herein are stable and versatile protein nanoparticles having a range of tunable fluorescent properties. Such nanoparticles may find utility in biological imaging. Methods of synthesis of such nanoparticles are also disclosed.

Abstract: Method, kit and composition for analyzing analytes for modifications using modification site specific antibodies to bind an analyte with his specific modification sites of interest to different dyes simultaneously with an antibody which is specific to the non-modificated analyte binding to another dye to determine the concentration of the analyte for quantification of the modified analyte in the identical sample.

Abstract: The present invention relates to a diagnostic method for the detection of small quantities of amniotic fluid in the vagina. More specifically, the invention relates to the detection of PAMG-1 in the vagina using anti-PAMG-1 antibodies.

Abstract: The present invention generally relates to methods for the detection of enzymes using elemental analysis.

Abstract: This document relates to methods for detecting and quantifying heavy and light chains of immunoglobulin using mass spectrometry techniques.

Abstract: In various implementations, a noninvasive, veterinary diagnostic test may be utilized to identify one or more immunologic food sensitivities. A fecal sample may be obtained. The veterinary diagnostic test may be performed on the obtained fecal sample. One or more immunologic food sensitivities may be identified based on the veterinary diagnostic test.

Abstract: Contemplated compositions, devices, and methods are drawn to various antigens from the pathogen M. tuberculosis and their use in vaccines, therapeutic agents, and various diagnostic tests.

Abstract: The present invention relates to methods of prognosing the survival of a diseased subject, particularly a subject with chronic kidney disease (CKD), as well as selecting an end-stage renal disease subject for a kidney transplant. The methods involve detecting an elevated amount of macrophage inhibitory cytoking-1 (MID-1) in a test body sample from the diseased subject. A method of preventing or reducing the risk of death in a CKD subject which involves removing or inactivating MIC-1 present in the blood, plasma or serum of the subject, is also disclosed.

Abstract: Method for determination of whether a subject suffers from intermittent atrial fibrillation based on the determination of the amount of a Troponin T in a sample in a patient suffering from atrial fibrillation using a binding assay. Kits and devices adapted to carry out the methods are also provided.

Abstract: The present invention provides compounds that are surrogates of post-translationally modified proteins and uses thereof. Numerous diseases are associated with post-translationally modified proteins that are difficult to obtain in homogenous form and in quantities needed for immunization and use as convenient standards, calibrators, and/or reference compounds that facilitate the detection and analysis of endogenous post-translationally modified proteins. The surrogate compounds of the invention typically comprise antigenic epitopes (one of which carries a post-translational modification) that are tethered by a flexible and hydrophilic linker. The resulting compound behaves like a surrogate of the post-translationally modified protein because it preserves the character of the included antigens and allows recognition by specific antibodies targeting the individual antigens.

Abstract: The disclosure provides biomarker proteins, a change in the concentration or activity level of which are associated with atypical hemolytic uremic syndrome (aHUS) or clinically meaningful treatment of aHUS with a complement inhibitor. Also provided are compositions and methods for interrogating the concentration and/or activity of one or more of the biomarker proteins in a biological fluid. The compositions and methods are useful for, among other things, evaluating risk for developing aHUS, diagnosing aHUS, determining whether a subject is experiencing the first acute presentation of aHUS, monitoring progression or abatement of aHUS, and/or monitoring response to treatment with a complement inhibitor or optimizing such treatment.

Abstract: Biomarkers, methods, and systems for assessment of traumatic brain injury of different severities, as well as treatment efficacy and blood brain barrier or blood cerebrospinal fluid integrity and assessment of neurodegenerative conditions. The methods include detecting in a patient sample one or more of ubiquitin C-terminal hydrolase LI (UCH-L1), glial fibrillary acid protein (GFAP), aldehyde dehydrogenase 1 family member LI (ALDHILI), phosphorylated neurofilament heavy chain (pNFH), medium chain (NFM), or light chain (NFL), alpha-synuclein, visinin-like protein 1 (VILIP-1) and S100B.

Abstract: The present invention relates to a polypeptide carrying a human BNP(1-32) epitope according to formula (I): a1-R1-X1-FGRKMDR-X2-R2-a2??(I) as well as ligands specific of the FGRKMDR epitope.

Abstract: The present invention relates to the use of a derivative of melatonin in an assay, wherein said derivative is a conjugate at position 3 of melatonin's indole ring and wherein said conjugate comprises a linker of at least 2 carbon atoms, with the proviso that the conjugate does not comprise a polypeptide or protein antigen. The derivative preferably comprises 3-(2-ethylamidoglutaric acid)-5-methoxyindole (GUS) and is coupled to a carrier such as dextran. The invention further relates to a method for detecting and/or quantifying melatonin in a sample using a compound comprising said derivative of melatonin, a corresponding immunobiological assay and a kit of parts for detecting and/or quantifying melatonin based on the melatonin derivative.

Abstract: Compositions and methods for measuring coagulation parameters using very small volumes of blood are provided. Advantageously, the methods described herein can be performed from a single drop of blood (about 20 ?L) while generally leaving enough sample to perform other measurements, optionally in a multiplexed format. The methods and devices do not require a skilled operator and can be performed at the point of service, which can be an important feature for managing blood coagulation disorders and treatments thereof.

Abstract: Methods, compositions, systems, and devices are provided for performing and analyzing agglutination assays. In one aspect, methods for image analysis of agglutination assays are provided. In another aspects, methods for performing agglutination assays are provided. In one aspect, the methods may be used for the detection of various molecules, including viruses or antibodies against a virus. In another aspect, the methods can be used to determine effective immunization of a subject.

Abstract: A microchip for testing a blood property, comprising first and second channels for allowing a blood sample(s) to flow inside, wherein the first channel has a first reaction section coated with collagen and tissue thromboplastin; and said second channel has a second reaction section, wherein the second section has been coated with collagen and tissue thromboplastin the amount of which is equivalent to or smaller than the amount of tissue thromboplastin on said first channel, or the second section has been coated with collagen but not with tissue thromboplastin.

Abstract: To provide a technology that allows for simple and highly accurate evaluation of the condition of blood. Provided is a blood condition analysis device, for analyzing the condition of a blood sample having added thereto at least one kind of drug selected from the group consisting of anticoagulation treatment canceling agents, coagulation activators, anticoagulants, platelet activators, and antiplatelet agents, the device including a drug dosage control unit for controlling the dosage of the drug to be added to the blood sample on the basis of the hematocrit and/or hemoglobin content of the blood sample.

Abstract: A processing system for processing experiment of biochemistry, biology and/or biotechnology includes a manual pipette which includes a piston and suctions and discharges liquid when the piston of the manual pipette is moved by external drive force, an automatic pipette which includes a piston and a built-in actuator and suctions and discharges liquid when the piston of the automatic pipette is moved by the actuator, a robot including a robotic arm which selects and grasps the manual or automatic pipette based on an operation command, and a robotic arm control device including circuitry which controls the robot such that the robotic arm selects and grasps the manual or automatic pipette based on the operation command. The operation command includes a collection of jobs that controls processes for processing a processing target in an experiment of biochemistry, biology and/or biotechnology and a container containing the processing target in a processing order.

Abstract: A system for automated microorganism identification and antibiotic susceptibility testing comprising a reagent cartridge, a reagent stage, a cassette, a cassette, stage, a pipettor assembly, an optical detection system, and a controller is disclosed. The system is designed to dynamically adjust motor idle torque to control heat load and employs a fast focus process for determining the true focus position of an individual microorganism. The system also may quantify the relative abundance of viable microorganisms in a sample using dynamic dilution, and facilitate growth of microorganisms in customized media for rapid, accurate antimicrobial susceptibility testing. Automated quality control test components and methods of their use are also disclosed.

Abstract: A method for automated microscopic analysis wherein the test protocol is obtained from interrogatable data affixed to each microscope slide. The method further comprises the algorithms that implement the test protocol.

Abstract: Techniques and architecture are disclosed for providing an optical system having an on-axis, internally mounted inertial measurement unit (IMU). In some cases, an IMU may be mounted within an interior region/cavity of an inner housing, which intern is configured to rotate within, an outer housing. In some instances, a mirror assembly may be operatively coupled with the inner housing and permitted to rotate simultaneously with the IMU. Rotation of the inner housing may be achieved, in some example cases, by use of a suitable motor. In some instances, positioning componentry may be operatively coupled with one or more of the IMU and/or mirror assembly. Improvements in mechanical stability, system dimensions, and/or protection from external/environmental hazards may be realized, in some example cases.

Abstract: A rotation speed measuring system includes a magnetic sensor, an acceleration sensor, an end detecting unit, and a calculating unit. The magnetic sensor measures earth magnetism in at least one axis direction. The acceleration sensor measures acceleration in at least one axis direction. The end detecting unit detects a time point of a switch at which an acceleration variation is switched to a large-variation state after a small-variation state, wherein the small-variation state corresponds to a state in which the acceleration variation is equal to or below a predetermined first threshold value and the large-variation state corresponds to a state in which the acceleration variation is equal to or above a predetermined second threshold value. The calculating unit calculates the rotation speed of the ball moving in midair by analyzing a frequency of measurement data on the earth magnetism acquired by the magnetic sensor until the end time point.

Abstract: A speed sensor of a rotatable output element of a transmission comprises a rotating tone wheel (33) and an inductive pick-up (29) comprising one of a drain plug, a fill plug or a level plug. The arrangement is suitable for providing a vehicle speed signal to a vehicle tachograph.

Abstract: The present invention provides a device and a method which allow a travel direction of a moving body to be estimated more efficiently.

Abstract: An accelerometer as disclosed herein includes a support wafer, a bottom wafer, a top wafer, and an inductive pick-off. The support wafer may define a plane and may comprise a first side, a second side, and a proof mass. The proof mass may be configured to move in the plane defined by the support wafer. The bottom wafer may comprise a first side and a second side, and the first side may be positioned over the first side of the support wafer. The top wafer may comprise a first side and a second side, and the first side may be positioned over the second side of the support wafer. The inductive pick-off may comprise a near field resonant conductive coupling mechanism and may be configured to output a signal indicative of an amount of displacement of the proof mass to electronics.