Abstract: A lubricant including an ionic liquid, which includes a conjugate acid (B+) and a conjugate base (X?), and is protic, wherein the conjugate acid includes a straight-chain hydrocarbon group having 10 or more carbon atoms, and wherein the conjugate base is represented by the following general formula (1): where n is an integer of from 0 to 6 in the general formula (1).

Abstract: A lubricating oil composition which contains, or is made by admixing: (A) an oil of lubricating viscosity, in a major amount; (B) at least one oil-soluble or oil-dispersible sulfurized fatty acid ester, as an additive in an amount providing the lubricating oil composition with greater than or equal to 0.02 mass % sulphur; and, (C) at least one oil-soluble or oil-dispersible alkali metal or alkaline earth metal salicylate detergent, as an additive in an effective minor amount, providing greater than or equal to 5 mmol of salicylate soap per kilogram of the lubricating oil composition, wherein the lubricating oil composition does not contain a phosphorus ester additive.

Abstract: Disclosed is a lubricating oil composition comprising the reaction product of a nitrogen containing reactant wherein the nitrogen containing reactant comprises an alkyl di-alkanolamide, an alkyl di-alkanolamine, or mixtures thereof; an acidic molybdenum compound; and a salicylate compound. Also disclosed is a method for operating an internal combustion engine comprising said lubricating composition and a method for preparing a molybdenum containing friction modifier.

Abstract: The present invention relates to functional fluid compositions containing friction modifiers, and specifically stable compositions containing friction modifiers with limited solubility in and/or limited compatibility with the functional fluids with which they are used. In particular the present invention deals with functional fluids used in internal combustion engines, such as engine oils, and friction modifiers that contain one or more amide functional groups, where the friction modifier is present in the functional fluid composition at levels that would otherwise cause the composition to be unstable and/or hazy.

Abstract: A sump-lubricated, spark-ignited engine is lubricated by supplying thereto a lubricant which comprises (a) an oil of lubricating viscosity; (b) a polyether; and (c) a metal-containing detergent. The lubricant has a total phosphorus content of less than 0.075 percent by weight.

Abstract: A process for increasing oil yield from grain that includes passing a grain-based liquid stream of an alcohol production process through a cavitation apparatus to apply cavitational energy to the grain-based liquid stream, wherein the cavitational energy is applied to the grain-based liquid stream prior to a distillation phase of the alcohol production process.

Abstract: The present invention relates to a method for fractionating a starting material containing a protein and lipids or phospholipid. The method comprises providing the starting material containing the protein and the lipids or phospholipid, extruding the starting material into first solvent having a temperature of 20-200° C. to form a solid phase of a texturized matrix enriched with the protein and not soluble in the first solvent and a lipids-enriched or phospholipid-enriched fluid phase, and collecting, separately, the solid phase of the texturized matrix enriched with the protein and the lipids-enriched or phospholipid-enriched fluid phase.

Abstract: The present invention relates to a composition comprising at least one phospholipid-splitting enzyme and at least one protease. The invention further relates to a method for degumming triglyceride-containing compositions by use of the composition according to the invention and to the use of said composition for degumming triglyceride-containing compositions.

Abstract: The present invention relates to compositions comprising polypeptides having phospholipase C activity and to the use of such compositions for hydrolysing a phospholipid or lysophospholipid, The invention also relates to processes for hydrolysing a phospholipid or lysophospholipid, comprising contacting said phospholipid or lysophospholipid with a composition or a pluralities of polypeptides having Phospholipase C activity according to the invention.

Abstract: The present invention relates to a compound of formula (I) in the form of any one of its stereoisomers or a mixture thereof which is useful as perfuming ingredients.

Abstract: Provided is a lens solution which has excellent lipid detergency, exhibits high hydrophilization performance, and exhibits an excellent lipid adhesion preventive effect and an excellent lubricity-imparting effect when the solution is used to coat a lens. Disclosed is a lens solution containing a polymer having 2.5% to 95% by mass of the following repeating unit (A) and 2.5% to 95% by mass of the following repeating unit (B): (A) a hydrophilic repeating unit; and (B) a repeating unit having a polyoxyalkylene group in a side chain and having the end of the side chain formed from an alkyl group having 5 to 30 carbon atoms, an alkanoyl group having 5 to 30 carbon atoms, or an aryl group.

Abstract: A composition for waste drain maintenance and the method of use thereof is presented. A composition of dry ingredients, including worm grind, vinegar, citrus rind, citric acid, brown sugar, baking soda, and fruit extract is provided. When placed in a drain and activated by water, enzymatic action occurs that breaks down and dissolves many of the greases, fats, and particulate matter that tends to adhere to the inner surface of drain pipes, slowing discharge and eventually leading to clogs. Placement of a couple of tablespoons of the composition into a drain, followed by about a quart of water, and leaving the same to stand for a period of time, such as overnight, allows for the decomposition of the offensive material on the drain walls. The dissolved, decomposed material can then be flushed away as an effluent by the addition of an adequate quantity, such as a gallon, of fresh water.

Abstract: The present invention is directed towards a process for making a powder or granule containing (A) at least one chelating agent selected from methyl glycine diacetic acid (MGDA) and glutamic acid diacetate (GLDA) and iminodisuccinic acid (IDS) and their respective alkali metal salts, (B) at least one homo- or copolymer of (meth)acrylic acid, partially or fully neutralized with alkali, said process comprising the steps of (a) mixing the at least one chelating agent (A) and the at least one homo- or copolymer (B) in the presence of water, (b) removing most of said water by spray-drying or spray granulation using a gas with an inlet temperature of at least 125° C.

Abstract: A composition for use on a hard surface. The composition has: (i) at least 7.5 wt. % of at least one surfactant selected; (ii) a transport rate factor of less than about 55 seconds; and (iii) an adhesion time of greater than about 8 hours.

Abstract: A container assembly (10) for retaining a liquid (14) during aging of the liquid (14) includes a container (12), a first flavor insert (16), and an insert retainer assembly (18). The container (12) defines a chamber (12A) which receives and retains the liquid (14). The first flavor insert (16) imparts a flavor on the liquid (14). The insert retainer assembly (18) is positioned within the chamber (12A) and is secured to the container (12). The insert retainer assembly (18) includes an alignment rack (30) and a retainer rack (32). The alignment rack (30) includes a first alignment guide (30C) that guides the first flavor insert (16) during positioning of the first flavor insert (16) within the container (12). The retainer rack (32) has a first attacher (32C) that selectively and individually engages the first flavor insert (16) to inhibit large scale movement of the first flavor insert (16) relative to the retainer rack (32).

Abstract: A multilayered cell culture apparatus for the culturing of cells is disclosed. The cell culture apparatus is defined as an integral structure having a plurality of cell culture chambers in combination with tracheal space(s). The body of the apparatus has imparted therein gas permeable membranes in combination with tracheal spaces that will allow the free flow of gases between the cell culture chambers and the external environment. The flask body also includes an aperture that will allow access to the cell growth chambers by means of a needle or cannula. The size of the apparatus, and location of an optional neck and cap section, allows for its manipulation by standard automated assay equipment, further making the apparatus ideal for high throughput applications.

Abstract: Drop-to-drop connection schemes are described for putting a microfluidic device in fluidic communication with a fluid source or another microfluidic device, including but not limited to, putting a microfluidic device in fluidic communication with the perfusion manifold assembly.

Abstract: The present set of embodiments relate to a system, method, and apparatus for hydrating and mixing a liquid medium from a dry medium. Such systems, methods, and apparatuses can be used in the biotech industry because they can be used to provide prepackaged dry media in a format that can be quickly rehydrated free of issues with operator error found in more conventional media rehydration systems using mixing tanks and reactors.

Abstract: A fluid-containing cartridge for a tissue processing apparatus includes a body defining a plurality of discrete fluid passageways, and a plurality of fluid-containing wells that are disposed on the body, wherein each fluid passageway of the body defines a fluid path between one of the fluid-containing wells and a fluid exit port that is configured to dispense fluid onto a laboratory slide.

Abstract: Devices, systems, and methods for facilitating placement of cells and materials in culture plates configured for high-throughput applications are provided. A culture system is provided with a culture plate having a lid for guiding placement of cells and materials in each individual culture well of a culture plate. The lid may provide for coupling to an electrophysiology culture plate comprising a biosensor plate and a biologic culture plate, where the biosensor plate underlies and is coupled to the culture well plate such that each biosensor is operatively coupled to one culture well of the plurality of culture wells. A containment device that physically influences the positioning of fluid received in the culture plate is also provided herein.

Abstract: An anaerobic fermentation with venturi stirring equipment has a container, a circulation unit, and a spray pipe. The container has at least one opening. The circulation unit communicates with the container and has a pump, a pumping pipe, a conveying pipe, and at least one storage tank. The pump is disposed out of the container. The pumping pipe communicates with the pump and a top of the container. The conveying pipe communicates with the pump and is mounted through one of the at least one opening, and the conveying pipe is located in the container. A gas valve is mounted on the conveying pipe. The at least one storage tank is mounted on the conveying pipe. The spray pipe is mounted in the container and communicates with the conveying pipe. The anaerobic fermentation with venturi stirring equipment can promote the fermenting rate and the ecological distribution of the anaerobic bacteria.

Abstract: A perfusion manifold assembly is described that allows for perfusion of a microfluidic device, such as an organ on a chip microfluidic device comprising cells that mimic cells in an organ in the body, that is detachably linked with said assembly so that fluid enters ports of the microfluidic device from a fluid reservoir, optionally without tubing, at a controllable flow rate. A culture module is contemplated that allows the perfusion and optionally mechanical actuation of one or more microfluidic devices, such as organ-on-a-chip microfluidic devices comprising cells that mimic at least one function of an organ in the body.

Abstract: An optofluidic photoreactor including an optical waveguide having an input, characterized by an evanescent optical field confined along an outer surface of the optical waveguide produced by radiation propagating in the optical waveguide, means for inputting light to the input of the optical waveguide, and a photoactive material disposed substantially only within the evanescent field. A method for optically activating a photoactive material in an optofluidic photoreactor to convert carbon dioxide and water into other molecules that may be useful as a fuel or a chemical feedstock.

Abstract: Provided is a cell stimulating system including an oscillator, in an ultrasound probe array type, including a plurality of ultrasound devices disposed in a matrix structure, the oscillator being produced using a micro electro mechanical system (MEMS), a plurality of cell containers configured to each contain a cell that is selectively stimulated by the ultrasound devices, the cell containers being disposed on a top of the oscillator to correspond to each of the ultrasound devices, and a device operator configured to operate an ultrasound device selected from among the ultrasound devices. According to an embodiment of the present invention, it is possible to selectively and simultaneously stimulate the cell of each of the cell containers based on a plurality of parameters, by selective operation of the ultrasound devices of the oscillator by the device operator, such that various experiments are simultaneously performed while reducing cost and an amount of time used for the experiments.

Abstract: A system including one or more rotatable bodies having an underlying structure resembling a double helix may be arranged and used, to produce useful electromagnetic effects for various applications.

Abstract: System and methods are provided for initiating a cell culture. The systems and methods include a first enclosure containing a thermal mass positioned on a surface area of a platform. The systems and methods include a second enclosure, containing a culture fluid and a gas mixture, placed on the first enclosure such that the first enclosure is positioned between the second enclosure and the platform. The systems and methods further include a cover secured on the second enclosure, and generating first thermal energy on the surface area of the platform and second thermal energy from the cover.

Abstract: Functionalized substrate materials, for example inorganic particles and/or synthetic polymeric particles, are used to enhance bioprocesses such as saccharification and fermentation.

Abstract: The present invention relates to a method for producing biomass feedstock, by cultivating green microalgae cells in which the expression and/or the activity of the DYRKP-1 protein is altered, inducing reserve accumulation and/or increase in bio-mass production by said microalgae.

Abstract: The invention relates to a bacterial strain belonging to the genus Rhodococcus which is a producer of a nitrile hydratase. The invention also relates to a method for producing acrylamide by hydration of acrylonitrile using a biomass of the bacterial strain and to a method of culturing the bacterial strain.

Abstract: Cell culture media, concentrated media and feeds, methods of manufacturing cell culture media and feeds, and methods of culturing cells are provided. One or more small peptides, including dipeptides are added to the cell culture media to provide improved stability and improved conditions for culturing cells.

Abstract: A culture module is contemplated that allows the perfusion and optionally mechanical actuation of one or more microfluidic devices, such as organ-on-a-chip microfluidic devices comprising cells that mimic at least one function of an organ in the body. A method for pressure control is contemplated to allow the control of flow rate (while perfusing cells) despite limitations of common pressure regulators. The method for pressure control allows for perfusion of a microfluidic device, such as an organ on a chip microfluidic device comprising cells that mimic cells in an organ in the body, that is detachably linked with said assembly, so that fluid enters ports of the microfluidic device from a fluid reservoir, optionally without tubing, at a controllable flow rate.

Abstract: The present invention relates to a composition for inducing direct transdifferentiation into oligodendrocyte progenitor cells (OPCs) from somatic cells, the composition containing at least one protein selected from the group consisting of direct transdifferentiation factors OCT4, SOX1, SOX2, SOX10, OLIG2, NKX2.2, and NKX6.

Abstract: A cell culture medium comprising adenosine triphosphate; a carrier protein; cholesterol, linoleic acid, and lipoic acid; glutathione; at least one nucleotide salvage pathway precursor base; phosphoethanolamine; selenium; transferrin; triiodothyronine; all-trans-retinoic acid (ATRA) and vitamin C; zinc, magnesium, and copper; an agent that increases intracellular cAMP; epidermal growth factor (EGF); hydrocortisone; insulin; and charcoal stripped fetal bovine serum, wherein said cell culture medium is substantially free, if not entirely free, of vitamin D, androgenic hormones, androgenic ligands, estrogenic hormones, estrogenic ligands, and/or androgenic receptors.

Abstract: The present invention relates to a method for polypeptide transfer into cells. The present invention further relates to the detection of polypeptide-specific immune cells and the priming, expansion and reactivation of polypeptide-specific T cells. Moreover the present invention relates to polypeptides of the methods of the present invention in combination with urea and their use for research, diagnosis or treatment and prevention of diseases in animals and humans.

Abstract: Disclosed are methods, compositions of matter, and kits useful for augmentation of cells through modification of cellular membrane properties following ex vivo treatment.

Abstract: Applications in transfusion medicine requiring platelets, and hematopoietic stem-cell transplantations require either platelets or enhancement of in vivo platelet biogenesis. Gene therapy applications of hematopoietic stem and progenitor cells (HSPCs) require effective and specific modification of HSPCs by DNA, RNA or other biological molecules. Here we disclose methods for the generation, and modification of megakaryocytic microparticles (MkMPs) or microvesicles, that can be used in the aforementioned transfusion and transplantation medicine applications and in gene therapy applications involving hematopoietic stem cells. The biological effects of modified or unmodified MkMPs have never been previously disclosed and thus, this invention claims all biological applications of MkMPs in in vivo therapeutic applications or ex vivo applications to produce various cells and cell parts, modify various target cells or deliver molecules including drugs to HSPCs and related cells.

Abstract: Human somatic cells obtained from individuals with a genetic heart condition are reprogrammed to become induced pluripotent stem cells (iPS cells), and differentiated into cardiomyocytes for use in analysis, screening programs, and the like.

Abstract: Described herein are compositions and methods of forming multi-cellular tumoroids. Also described herein are methods of using the multi-cellular tumoroids.

Abstract: Compositions and methods to prepare influenza virus-like particles (VLPs) are provided.

Abstract: The present invention relates to a vaccine for protecting a piglet against diseases associated with a novel pestivirus. The vaccine commonly includes a pestivirus antigen and, optionally an adjuvant. Methods for protecting pigs against diseases associated with pestivirus, including but not limited to congenital tremors and methods of producing the pestivirus vaccine are also provided.

Abstract: This disclosure relates to the isolation of a nucleic acid molecule[s] that encode a novel cytochrome P450 and an oxidoreductase from a Papaver somniferum [P. somniferum] cultivar, transgenic cells transformed with said nucleic acid molecule and sequence variants thereof; and including methods for the production of intermediates in the production of morphinans.

Abstract: The present invention provides an in vitro directed evolution selection system to create modified methyltransferases which improve methyltransferase specificity and use it to optimize and provide fusion proteins comprising a zinc finger methyltransferase derived from M.SssI. The resulting fusion proteins show increased target methylation specificity and greatly decreased non-target methylation compared to wild-type enzyme activity. Methods of use of such fusion proteins in both prokaryotic and eukaryotic cells are also provided.

Abstract: Provided is a polynucleotide for encoding a protein that exhibits activity for transferring a sugar to a hydroxyl group at position 7 of a flavone, particularly flavone 4?-glucoside. A polynucleotide selected from the group consisting of: (a) polynucleotides comprising a base sequence of SEQ ID NO.: 1 or SEQ ID NO.: 5; (b) polynucleotides that hybridize, under stringent conditions, with polynucleotides comprising a base sequence complementary to a base sequence of SEQ ID NO.: 1 or SEQ ID NO.: 5, wherein the polynucleotides encode a protein that exhibits activity for transferring a sugar to a hydroxyl group at position 7 of a flavone; (c) polynucleotides for encoding proteins comprising an amino acid sequence of SEQ ID NO.: 2 or SEQ ID NO.: 6; (d) polynucleotides for encoding proteins comprising an amino acid sequence in which one or more amino acids have been deleted, substituted, inserted, and/or added in an amino acid sequence of SEQ ID NO.: 2 or SEQ ID NO.

Abstract: A formulation comprising an ingestible carrier and an isolated strain of Bifidobacterium longum.

Abstract: Engineered CRISPR-Cas9 nucleases with improved specificity and their use in genomic engineering, epigenotnic engineering, genome targeting, and genome editing.

Abstract: The present disclosure provides compositions and methods for enhancing directed nucleic acid repair, which are useful in the area of genome engineering.

Abstract: The present invention relates to methods for producing N-terminal derivatives of proteins in which a polysaccharide, preferably having at least terminal sialic units, and preferably consisting essentially only of sialic acid units, is reacted at the N-terminus of a protein or peptide under controlled conditions to produce an N-terminal derivative. The controlled conditions include use of acidic pH for the derivatisation step and a higher pH for purification. The derivatives are useful for improving pharmacokinetics and pharmacodynamics of proteins and peptides.

Abstract: The disclosure provides a nitrilase from Arabis alpina, which belongs to genus Arabis, family brassicaceae. The disclosure further provides the encoding gene, vector, recombinant bacterial strain, and the application in the manufacturing of (S)-3-cyano-5-methylhexanoic acid. The wet resting cells containing nitrilase Aa-Nit can kinetically resolve racemic IBSN at 1.2 M with a 42% conversion rate in 15 hr and >99% ee value. The disclosure provides a regio- and stereoselective method for the preparation of (S)-3-cyano-5-methylhexanoic acid. This method provides an atom economical, mild, environmental friendly industrial method to manufacture (S)-3-cyano-5-methylhexanoic acid.

Abstract: A method for determining past exposure to chemical agents or heavy metals may include coating a capture material with a capture reagent. The capture reagent may be selected based on an ability of the capture reagent to bind with a target antibody, and the target antibody may be an indicator associated with a particular chemical agent or heavy metal. The method may further include interrogating a clinical sample associated with an individual by forming a mixture of the capture material and the clinical sample, and determining an exposure status of the individual to the particular chemical agent or heavy metal based on whether the capture material demonstrates capture of the indicator.

Abstract: A library of replicating entities, each entity comprises a recombinant vector comprising a randomized nucleic acid sequence, having the reading frame structure [NXX]n [CorAA] [NXX]m [NZZ]o, or [NZZ]o [NXX]m [CorAA] [NXX]n. Each NXX is independently a codon encoding for any amino acid except cysteine, CorAA is a codon encoding for cysteine or at least one other amino acid, each NZZ is independently a codon encoding for any amino acid, and n is an integer from 0 to 40, m is an integer from 1 to 20, o is an integer from 1 to 40, and at least 20 percent of CorAA encode for cysteine. The invention further relates to a set of recombinant vectors and to a set of randomized oligonucleotides, each oligonucleotide having said structure, as well as to a method for generating a library of replicating entities and to a method for identifying an amino acid polymer.