Abstract: This invention provides a drug-inducible promoter that can be used for sugarcane plants and plants related thereto. Such drug-inducible promoter comprises a polynucleotide comprising a first nucleotide sequence having 90% or higher identity to the nucleotide sequence as shown in SEQ ID NO: 1, a second nucleotide sequence having 90% or higher identity to the nucleotide sequence as shown in SEQ NO: 2, a third nucleotide sequence having 90% or higher identity to the nucleotide sequence as shown in SEQ ID NO: 3, a fourth nucleotide sequence having 90% or higher identity to the nucleotide sequence as shown in SEQ ID NO: 4, and a fifth nucleotide sequence having 90% or higher identity to the nucleotide sequence as shown in SEQ ID NO: 5 in such an order from the 3 terminal side toward the 5? terminal side.

Abstract: Provided herein are Solanum lycopersicum plants with increased fruit yield and to seeds or fruits of the present Solanum lycopersicum plants. Also provided herein are methods for increasing the fruit yield of Solanum lycopersicum plants. Further provided are fruits and seeds of the present Solanum lycopersicum plants. Solanum lycopersicum plant with increased fruit yield including the SP3D and SP gene, or at least the promoter sequence thereof, of Solanum pennelli or another Solanum species such as Solanum neorickii, Solanum chmielewskii, Solanum chilense, Solanum parviflorum, Solanum pimpinellifolium, and Solanum peruvianum.

Abstract: Disclosed herein are transgenic cells expressing a heterologous nucleic acid encoding a prephenate dehydrogenase (PDH) protein, a heterologous nucleic acid encoding a homogentisate solanesyl transferase (HST) protein, a heterologous nucleic acid encoding a deoxyxylulose phosphate synthase (DXS) protein, or a combination of two or more thereof. In particular examples, the disclosed transgenic cells have increased plastoquinone levels. Also disclosed are methods of increasing cell growth rates or production of biomass by cultivating transgenic cells expressing a heterologous nucleic acid encoding a PDH protein, a heterologous nucleic acid encoding an HST protein, a heterologous nucleic acid encoding a DXS protein, or a combination of two or more thereof under conditions sufficient to produce cell growth or biomass.

Abstract: Compositions and methods for increasing disease resistance in plants, particularly tomato plants are disclosed.

Abstract: A protein conferring resistance to Fusarium Head Blight disease (FHB) is described, along with the DNA sequence of the corresponding gene and mRNA copy (cDNA). The cDNA of Fhb1 gene can be used to produce genetically-modified plants having increased resistance to FHB, particularly in wheat, barley and other plants affected by the disease. The protein has antifungal properties and inhibits fungal growth, thereby providing a means for reducing DON toxin in grains. Conserved functional domains are identified in the protein. Genetically-modified plants having increased resistance FHB are also described, along with methods for producing such genetically-modified plants.

Abstract: A virus-resistant tobacco in accordance with the present invention is such that it includes a mutation in a translation initiation factor eIF(iso)4E gene, the mutation causing production of an eIF(iso)4E protein which is non-functional with respect to a virus or suppressing expression of the eIF(iso)4E gene, or a virus-resistant tobacco in accordance with the present invention is such that an expression level of the eIF(iso)4E gene is 20% or lower as compared to a wild type.

Abstract: The present invention relates to novel gene sequences encoding insecticidal proteins produced by Bacillus thuringiensis strains. Particularly, new chimeric genes encoding a CryIC, CryIB or CryID protein are provided which are useful to protect plants from insect damage. Also included herein are plant cells or plants comprising such genes and methods of making or using them, as well as plant cells or plants comprising one of such chimeric gene and at least one other such chimeric genes.

Abstract: This disclosure concerns nucleic acid molecules and methods of use thereof for control of insect pests through RNA interference-mediated inhibition of target coding and transcribed non-coding sequences in insect pests, including coleopteran pests. The disclosure also concerns methods for making transgenic plants that express nucleic acid molecules useful for the control of insect pests, and the plant cells and plants obtained thereby.

Abstract: The invention provides for delivery, engineering and optimization of systems, methods, and compositions for manipulation of sequences and/or activities of target sequences. Provided are delivery systems and tissues or organ which are targeted as sites for delivery. Also provided are vectors and vector systems some of which encode one or more components of a CRISPR complex, as well as methods for the design and use of such vectors. Also provided are methods of directing CRISPR complex formation in eukaryotic cells to ensure enhanced specificity for target recognition and avoidance of toxicity and to edit or modify a target site in a genomic locus of interest to alter or improve the status of a disease or a condition.

Abstract: The present invention provides an HSV vector comprising an envelope comprising one or more mutant gB and/or gH envelope glycoproteins, whereby the HSV vector exhibits at least 25% increased rate-of-entry after 20 minutes when assayed at 30° C. or 37° C. in Vero cells after first incubating at 4° C. relative to a control HSV comprising wild-type gB and gH glycoproteins. The invention also provides a viral stock comprising the inventive HSV vector.

Abstract: This application discloses the compositions comprising biologically active synthetic nanoparticle constructs and methods of use thereof to modify gene expression including transcriptional activation and transcriptional repression.

Abstract: Provided herein are compositions and methods useful, inter alia, for the delivery of ribonucleoprotein complexes (e.g., Cas9/guide RNA complexes) into cells. The compositions and methods provided herein are particularly useful for the delivery of ribonucleoprotein complexes into pluripotent cells and lymphatic cells.

Abstract: A transgenic cat with a phenotype characterized by the substantial absence of the major cat allergen, Fel d I. The phenotype is conferred in the transgenic cat by disrupting the coding sequence of the target gene with a specialized construct. The phenotype of the transgenic cat is transmissible to its offspring.

Abstract: Disclosed are compositions and methods for directing proteins to specific loci in the genome and uses thereof. In one aspect, the disclosed methods allow for directing proteins to specific loci in the genome of an organism, including the steps of providing a DNA localization component and an effector molecule, wherein the DNA localization component and the effector molecule are capable of being operatively linked via a non-covalent linkage.

Abstract: Provided herein are plants having altered expression of an IRX10 or an IRX10-L protein. Such plants have phenotypes that may include decreased recalcitrance, increased growth, decreased lignin content, or a combination thereof. Also provided herein are methods of making and using such plants.

Abstract: A method for enhancing yeast growth for bioproduct production is described. A method for fermentative bioproduct production also is provided. A nutrient composition used in the methods also is described. A liquid mixture containing the nutrient composition, yeast culture (or fungi, algae, or bacteria culture), and sugars also is provided.

Abstract: A microorganism capable of producing 1,4-butanediol and a method of producing 1,4-butanediol using the same.

Abstract: [Problems] To provide a method of producing a medium chain fatty acid or a lipid containing this fatty acid as a component by using a ?-ketoacyl-ACP synthase, and a gene, a protein and a transformant for use in this method. [Means to solve] A method of producing a medium chain fatty acid or a lipid containing this fatty acid as a component, containing the steps of: introducing a gene encoding the following protein (A) or (B) into a host, and thereby obtaining a transformant, and collecting a medium chain fatty acid or a lipid containing this fatty acid as a component from the resulting transformant; and a gene, a protein and a transformant for use in this method: (A) A protein consisting of the amino acid sequence set forth in SEQ ID NO: 1; and (B) A protein consisting of an amino acid sequence having 90% or more identity with the amino acid sequence set forth in SEQ ID NO: 1, and having medium chain acyl-ACP-specific ?-ketoacyl-ACP synthase activity.

Abstract: Described is a method for the enzymatic production of acetyl phosphate from formaldehyde using a phosphoketolase or a sulfoacetaldehyde acetyltransferase.

Abstract: The present invention provides a process for the treatment of a composition comprising yeast cell walls comprising ?-1,3-glucans which are insoluble when extracted with water and partially soluble when extracted with DMSO, the process comprising contacting said composition with laminaripentaose-producing-?-1,3-glucanase and inactivating the laminaripentaose-producing-?-1,3-glucanase to result in a composition comprising yeast cell walls wherein the ?-1,3-glucans have an improved solubility in DMSO and the ratio of ?-glucans soluble in DMSO compared to water is greater than or equal to 2.

Abstract: The present teachings provide methods of converting cellulosic materials to soluble sugars. Methods for increasing the yield of glucose from the enzymatic saccharification of cellulosic materials is also provided. The present teachings further provide methods of increasing the yield of cellobiose from the enzymatic saccharification of cellulosic materials.

Abstract: A method of synthesizing a nucleic acid complementary to a target nucleic acid sequence in a template nucleic acid includes annealing a swarm primer to a target nucleic acid, the swarm primer overlapping an F1 site of the target nucleic acid and extends toward the F2 site of the target nucleic acid. An inner primer may also be annealed to the target nucleic acid to produce a complimentary nucleic acid having a single-strand loop onto which further primers may anneal. A plurality of amplicons may be reproduced, many of which may have further primers annealed thereto to generate more complementary nucleic acids.

Abstract: The current invention is directed to a method for obtaining a nucleic acid encoding an immunoglobulin variable domain from a single cell comprising the following steps: performing a first polymerase chain reaction with three to six 5?-primer and one 3?-primer, performing with the product of the first polymerase chain reaction a second polymerase chain reaction with thirteen to sixteen 5?-primer and one 3?-primer, whereby the distance of the binding locations of the primer employed in the second polymerase chain reaction is reduced compared to the first polymerase chain reaction.

Abstract: Provided are a method of preparing an antibody with a regulated, sugar chain content, the method including the step of culturing antibody-expressing cells in a medium including glycerol as an additive for regulating the antibody sugar chain content, a method of preparing a high-quality population of antibodies by regulating the sugar content of the antibody to a desired content, and a population of antibodies prepared by the method. Further, provided is a method of regulating the antibody sugar chain content, the method including the step of culturing antibody-expressing cells in a medium including glycerol as an additive for regulating the antibody sugar chain content. Furthermore, provided is a medium composition, for regulating the antibody sugar chain content, the medium composition including glycerol as an additive for regulating the antibody sugar chain content.

Abstract: A method of cell culture comprising providing cells in a cell culture medium to start a cell culture process, and, maintaining at least one metabolite selected from 3-(4-hydroxyphenyl)lactate, 4-hydroxyphenylpyruvate, phenyllactate, indolelactate, indolecarboxylic acid, homocysteine, 2-hydroxybutyric acid, isovalerate and formate below a concentration C1 in the cell culture medium, wherein C1 is 3 mM and/or (ii) maintaining at least one amino acid selected from phenylalanine, tyrosine, tryptophan, methionine, leucine, serine, threonine and glycine below a concentration C2 in the cell culture medium, wherein C2 is 2 mM.

Abstract: The invention provides the mammalian cell culture process for the production of monoclonal antibodies and fusion proteins wherein the mammalian cell is cultured in suitable cell culture conditions specifically maintaining monophasic temperature.

Abstract: A method for increasing efficiency of a method for producing astaxanthin by culturing a microalga. A method for producing astaxanthin in which astaxanthin is produced in an algal body by culturing a microalga, wherein photoirradiation is performed using both a blue LED of peak wavelength from 420 to 500 nm and a red LED of peak wavelength from 620 to 690 nm, at least during an astaxanthin-producing culturing phase of a culturing period. The ratio of the blue LED of peak wavelength from 420 to 500 nm and the red LED of peak wavelength from 620 to 690 nm is preferably from 1:19 to 19:1 by photon flux density, and the photon flux densities are each preferably not less than 20 ?mol/m2/s.

Abstract: An enzyme electrode includes an electrode, and a detection layer which contacts the electrode and contains a crosslinking agent, an electrically conductive macromolecule and an enzyme transferring and receiving electrons to and from the electrode and does not contain an electron transfer subunit.

Abstract: Methods for diagnosing, treating, and monitoring the treatment of invasive aspergillosis (IA) are described. The methods can include detecting the presence of one or more volatile organic compounds (VOCs) in the breath of subjects suspected of having IA.

Abstract: Methods, apparatuses, and systems for microorganism strain analysis of complex heterogeneous communities, predicting and identifying functional relationships and interactions thereof, and selecting and synthesizing microbial ensembles based thereon are disclosed. Methods for identifying and determining the absolute cell count of microorganism types and strains, along with identifying the network relationships between active microorganisms and metadata, are also disclosed.

Abstract: An apparatus and method of using an optical coherence elastography (OCE) under acoustic radiation force (ARF) excitation includes the steps of inducing an excitation wave in a blood sample by use of an ultrasound beam from an ultrasonic transducer; measuring an elastic property of the blood sample by use of an optical coherence tomography (OCT) beam transverse to the ultrasound beam to dynamically measure the elastic property of the blood sample during coagulation and assessing the clot formation/dissolution kinetics and strength.

Abstract: A nucleic acid amplification reagent includes a first probe which anneals to a target nucleic acid in a region sandwiched between a site to which the 3? end of a forward primer anneals and a site to which the 3? end of a reverse primer anneals in a template nucleic acid, and a second probe which anneals to a nucleic acid other than the target nucleic acid in the region. Each of the first probe and the second probe includes a dye which emits light in a wavelength band mutually overlapping partially.

Abstract: A method of enriching for a fragment of a genome, as well as corresponding compositions and kits, are provided. In certain embodiments, the method comprises: (a) contacting a sample comprising fragmented DNA with a Cas9-gRNA complex comprising mutant Cas9 protein that has inactivated nuclease activity and a Cas9-associated guide RNA that is complementary to a site in the DNA, to produce a Cas9-fragment complex that comprises a fragment of the fragmented DNA; and (b) isolating the complex. In addition, other methods and compositions for Cas9/CRISPR-mediated nucleic acid manipulation are also provided.

Abstract: The present invention relates to a scalable multiplex PCR method that can simultaneously amplify overlapping amplicons without the drawbacks of conventional multiplex PCR. The method selectively amplifying target nucleic acid fragments having an overlapping region. The method comprises the steps of: obtaining a first nucleic acid sequence comprising a first tag t2 and a first forward primer F1, obtaining a second nucleic acid sequence comprising a second tag t1 and a first reverse primer R1, obtaining a third nucleic acid sequence comprising the second tag t1 and a second forward primer F2, obtaining a fourth nucleic acid sequence comprising a third tag t3 and a second reverse primer R2, wherein each primer is a gene-specific primer; performing initial cycles of PCR; and then performing later cycles of PCR at higher annealing temperatures to obtain amplification products.

Abstract: Methods of sequencing molecules based on luminescence lifetimes and/or intensities are provided. In some aspects, methods of sequencing nucleic acids involve determining the luminescence lifetimes, and optionally luminescence intensities, of a series of luminescently labeled nucleotides incorporated during a nucleic acid sequencing reaction.

Abstract: A method for analyzing planar sample is provided. In some cases the method comprises: (a) labelling the planar sample with a capture agent that is linked to a nucleic acid, wherein the capture agent specifically binds to complementary sites in the planar sample; (b) reading a fluorescent signal caused by extension of a primer that is hybridized to the nucleic acid, using fluorescence microscopy. Several implementations of the method, and multiplexed versions of the same, are also provided.

Abstract: The present invention relates to compositions which may be used as controls and/or references for quantitative and/or semi-quantitative detection methods, in particular for digital PCR or next generation sequencing assays. The present invention also describes synthetic nucleic acid constructs, in particular plasmids which are present in said compositions, kits, their uses and a method involving the use of the compositions according to the present invention. Furthermore, methods for providing the compositions according to the present invention are described herein.

Abstract: In one embodiment, 3-Aminopropyltriethoxysilane (APTES) functionalized graphene oxide (APTES-GO) wrapped SiO2 particle composite (SiO2@APTES-GO) was prepared via the self-assembly process of APTES-GO sheets and SiO2 particles. Transmission electron microscopy (TEM) and Attenuated Total Reflectance-Fourier Transform Infrared spectroscopy (ATR-FTIR) confirmed wrapping of the SiO2 particles by the APTES-GO sheets. A biosensor based on electrochemical impedance spectroscopy (EIS) was constructed and used to sensitively detect dengue DNA and dengue RNA via primer hybridization using different oligonucleotide sequences. The results demonstrated that the SiO2@APTES-GO electrode material led to enhanced sensitivity, selectivity and detection limit, compared to both APTES-GO and APTES-SiO2.

Abstract: Methods for attaching barcodes to polypeptides are provided. Methods for detecting molecular interactions at the single molecule level are provided. Embodiments of the invention are directed to a ONA barcoded protein array technology for parallel protein interaction profiling on a single molecule basis. DNA barcodes are attached to proteins collectively via ribosome display or individually via enzymatic conjugation. Novel methods are described herein that measure protein interactions based on the statistical analysis of co-localized polonies arising from barcoding DNAs of interacting proteins.

Abstract: The present disclosure relates to the amplification of target nucleic acid sequences for various sequencing and/or identification techniques. The use of these primers, as described herein, allows for the reduction in the amplification of nonspecific hybridization events (such as primer dimerization) while allowing for the amplification of the target nucleic acid sequences.

Abstract: An oligonucleotide having a novel structure and a method of synthesizing nucleic acid uses the same as a primer. This oligonucleotide is provided at the 5?-side of the primer with a nucleotide sequence substantially the same as a region synthesized with this primer as the origin of synthesis. The present invention realizes synthesis of nucleic acid based on an isothermal reaction with a simple constitution of reagents. Further, the method synthesizes highly specific nucleic acid on the basis of this method of synthesizing nucleic acid.

Abstract: The invention relates to new methods of attaching one or more polynucleotide binding proteins to a target polynucleotide. The invention also relates to new methods of characterising target polynucleotides.

Abstract: Compositions, methods and systems are provided for isolating DNA having a modified or unnatural base. Circular DNA fragments, each comprising a double stranded DNA central region and single stranded regions on the ends of the double stranded regions, are obtained. Some of the fragments have one or more modified or unnatural base. The DNA fragments are treated with a primer and a polymerase such that the polymerase extends the primer to copy at least one of the strand of the double stranded region. This results in rendering the other strand single stranded. A binding protein or antibody that is specific to the modified or unnatural base is then used to isolate strands containing the modified or unnatural bases. Methods for loading such complexes onto substrates and for single molecule sequencing of such complexes are also provided.

Abstract: Materials and methods provided herein are useful for determining an implantation threshold for a euploid embryo and for determining the potential of a euploid embryo to implant and initiate a pregnancy.

Abstract: The invention encompasses methods for increasing genetic progress in livestock, and for genetic dissemination, including the use of amniocentesis to obtain fetal amniocytes for use in genomic evaluation and cloning.

Abstract: The present invention relates to methods and systems for assessing ovarian reserve and function in a female subject and informing course of treatment thereof. The invention provides methods for assessing ovarian reserve and function by analyzing both clinical and genetic data/characteristics from a female subject. These methods involve the determination of the presence of one or more mutations in a gene, the gene being associated with fertility and/or ovarian reserve or function. In certain aspects the methods also involve the determination of one or more clinical characteristics associated with fertility and/or ovarian reserve or function. In certain embodiments, the clinical and genetic characteristics obtained from a female subject can be used as data to be input to an ovarian reserve predictor, such that a probability of the female subject suffering from ovarian reserve dysfunction or premature decline can be generated.

Abstract: The present invention relates to in vitro methods for the diagnosis of chronic obstructive pulmonary disease (COPD), wherein the expression of the marker gene DMBT1 is determined. In particular, the invention relates to an in vitro diagnostic method of assessing the susceptibility of a subject to develop progressive COPD involving the appearance of irreversible lung damage, wherein the expression of the marker gene DMBT1 and optionally one or more further marker genes selected from KIAA1199, TMSB15A, DPP6, SLC51B, NUDT11, ELF5, AZGP1, PRRX1, AQP3, SFN, GPR110, GDF15, RASGRF2, RND1, PLA1A, FGG, CEACAM5, HYAL2, AHRR, CXCL3, CYP1A1, CYP1B1, CYP1A2, CST6, NTRK2, COMP, ITGA10, CTHRC1, TAL1, FIBIN, BEX5, BEX1, ESM1 and GHRL is determined.

Abstract: Described are methods for early diagnosis and progression monitoring of Mild Cognitive Impairment (MCI) and Alzheimer's Disease (AD) by quantifying neurite and/or synapse miRNAs in bodily fluids.

Abstract: The invention provides methods, systems, and computer readable medium for detecting ploidy of chromosome segments or entire chromosomes, for detecting single nucleotide variants and for detecting both ploidy of chromosome segments and single nucleotide variants. In some aspects, the invention provides methods, systems, and computer readable medium for detecting cancer or a chromosomal abnormality in a gestating fetus.

Abstract: The invention provides methods of determining the aggressiveness, prognosis and response to therapy for particular cancers, which include comparing the expression levels of one or a plurality of differentially expressed genes from one or more 5 functional metagenes, including a Carbohydrate/Lipid Metabolism metagene, a Cell Signalling metagene, a Cellular Development metagene, a Cellular Growth metagene, a Chromosome Segregation metagene, a DNA Replication/Recombination metagene, an Immune system metagene, a Metabolic Disease metagene, a Nucleic Acid Metabolism metagene, a Post-Translational Modification metagene, a Protein 10 Synthesis/Modification metagene and a Multiple Networks metagene. The method disclosed herein may be particularly suitable as a companion diagnostic for cancer therapies.