Patents Issued in May 2, 2017
-
Patent number: 9637740Abstract: Described herein are methods of inhibiting mitosis, treating cancer and/or treating immune disorders through the use of agents that inhibit FAT 10 and/or the FAT 10 pathway.Type: GrantFiled: April 2, 2013Date of Patent: May 2, 2017Assignee: President and Fellows of Harvard CollegeInventors: Yifat Merbl, Marc W. Kirschner
-
Patent number: 9637741Abstract: This invention provides a method for modulating the expression of a first gene in a cell wherein the first gene is one containing more than 36 CAG trinucleotide repeats and encoding a protein that form polyglutamine-mediated protein aggregation. Suppression of the first gene is achieved by reducing the expression of SPT4 gene or SUPT4H gene. It can also be achieved by inhibiting the formation of a Spt4/Spt5 complex or a Supt4h/Supt5h complex. Also provided is a method for identifying an agent useful for modulating the expression and aggregation of CAG-expanded gene product, or treating a polyglutamine disease such as Huntington's disease.Type: GrantFiled: December 2, 2015Date of Patent: May 2, 2017Assignees: National Yang Ming University, The Board of Trustees of the Leland Stanford Junior UniversityInventors: Tzu-Hao Cheng, Chia-Rung Liu, Tzu-Han Wang, Stanley N. Cohen
-
Patent number: 9637742Abstract: The present invention relates to an RNAi-inducing nucleic acid molecule having a new structure and the use thereof, and more particularly to a novel nucleic acid molecule having a structure comprising a first strand, which is 24-121 nt in length and comprises a region complementary to a target nucleic acid, and a second strand which is 13-21 nt in length and has a region that binds complementarily to the region of the first strand, which is complementary to the target nucleic acid, so that the nucleic acid molecule inhibits the expression of a target gene with increased efficiency, and to a method of inhibiting the expression of a target gene using the nucleic acid molecule. The nucleic acid molecule structure of the present invention increases the efficiency with which the nucleic acid molecule inhibits the target gene.Type: GrantFiled: September 7, 2011Date of Patent: May 2, 2017Assignee: Sungkyunkwan University Foundation for Corporate CollaborationInventor: Dong Ki Lee
-
Patent number: 9637743Abstract: Provided is siRNA effective for the treatment of fibrosis and a pharmaceutical containing the siRNA.Type: GrantFiled: January 19, 2016Date of Patent: May 2, 2017Assignees: MIE UNIVERSITY, OTSUKA PHARMACEUTICAL CO., LTD.Inventors: Esteban C Gabazza, Tetsu Kobayashi, Hidekazu Toyobuku, Ayako Fukuda, Tetsuya Hasegawa
-
Patent number: 9637744Abstract: The present invention relates to methods and pharmaceutical compositions for the treatment of Erythropoietic Protoporphyria. In particular, the present invention relates to a method for increasing the amount of functional FECH in a erythroid cell carrying the hypomorphic allele IVS3 48C/T (rs2272783) in trans to a deleterious mutation in the FECH gene comprising the step of consisting of bringing the erythroid cell into contact with at least one antisense oligonucleotide (ASO) comprising the sequence as set forth by SEQ ID NO: 2 (5? gcagcctgagaaatgtttt 3?) to prevent splicing of the cryptic exon inserted into the mutant IVS3 48C/T (rs2272783) FECH mRNA.Type: GrantFiled: June 13, 2014Date of Patent: May 2, 2017Assignees: INSERM (INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE), UNIVERSITE PARIS DIDEROT—PARIS 7, UNIVERSITE DE VERSAILLES SAINT-QUENTIN-EN-YVELINES, ASSISTANCE PUBLIQUE-HOPITAUX DE PARIS (APHP)Inventors: Laurent Gouya, Jean-Charles Deybach, Herve Puy, Vincent Oustric
-
Patent number: 9637745Abstract: Connexin modulation for the treatment of wounds that do not heal at expected rates, including delayed healing wounds, incompletely healing wounds, and chronic wounds, and associated methods, compositions and articles.Type: GrantFiled: May 11, 2015Date of Patent: May 2, 2017Assignee: CoDa Therapeutics, Inc.Inventors: David Lawrence Becker, Colin Richard Green, Bradford James Duft
-
Patent number: 9637746Abstract: The invention pertains to a method for preparing cells that can be used as biocatalysts by inducing in them a growth-decoupled state, in which interferase inhibits the expression of genes except the ones that code for the pathway enzymes of interest. mRNAs that code for interferase-resistant products are overexpressed in the background of a metabolically-frozen cell. Enzymes that compete for a substrate or product of the pathway of interest may be altered such that the enzyme is sensitive to a site-specific protease, which protease is inducible in the host cell.Type: GrantFiled: December 14, 2009Date of Patent: May 2, 2017Assignee: GreenLight Biosciences, Inc.Inventor: Daniel Klein-Marcuschamer
-
Patent number: 9637747Abstract: Eukaryotic protein-coding messenger RNAs and non-coding microRNAs are naturally transcribed by type II RNA polymerases (pol-2) but not prokaryotic RNA polymerases. As a result, current eukaryotic RNA and protein production is performed either using eukaryotic pol-2 promoters in hybridomas or mammalian cells or using prokaryotic promoters in bacterial cells. However, because prokaryotic RNA transcription tends to be error-prone, frequent mutation is a big problem. Also, growing hybridomas or mammalian cells is relatively laborious and costly. To overcome these problems, the present invention provides a novel inducible composition and method for producing eukaryotic RNAs and/or their related peptides/proteins directly using eukaryotic pol-2 promoter-driven gene expression in fast growing bacteria, without the need of changing to prokaryotic promoters or growing hybridomas/mammalian cells.Type: GrantFiled: October 29, 2014Date of Patent: May 2, 2017Assignee: MELLO BIOTECHNOLOGY, INC.Inventors: Shi-Lung Lin, Donald C. Chang
-
Patent number: 9637748Abstract: The present invention provides novel C. botulinum conjugatively transmissible plasmids and methods of use thereof. Specifically, described herein are novel, conjugatively transmissible clostridial plasmids which are capable of being transferred among and between clostridial species. The novel plasmids of the present invention therefore permits the delivery of heterologous clostridial genes into a clostridial host, such as C. botulinum, and the expression of genes of interest in that host, including clostridial toxins and the nontoxigenic components of the toxin complex, toxin fragments, or antigenic portions thereof, in a way both that ensures abundant expression and facilitates purification. Furthermore, toxins with altered structures, chimeric, hybrid toxins, and other toxin derivatives valuable in medicine could be synthesized in this system.Type: GrantFiled: December 4, 2013Date of Patent: May 2, 2017Assignee: Wisconsin Alumni Research FoundationInventors: Eric A. Johnson, Kristin M. Marshall, Marite Bradshaw
-
Patent number: 9637749Abstract: Isolated mutant Mycobacterium tuberculosis bacteria comprising a deletion in the ESAT-6 gene cluster region 3 (esx-3 region) are provided, as well as compositions comprising such, methods of production thereof and methods of use thereof.Type: GrantFiled: May 10, 2013Date of Patent: May 2, 2017Assignee: Albert Einstein College of Medicine, Inc.Inventors: William R. Jacobs, JoAnn M. Tufariello
-
Patent number: 9637750Abstract: The present disclosure relates to inducible hybrid plant 5S rRNA mimic (P5SM) RNA elements that regulate gene expression by alternative splicing, and to methods of using such RNA elements for regulating transgene expression in eukaryotic host cells and plants.Type: GrantFiled: January 22, 2013Date of Patent: May 2, 2017Assignee: THE REGENTS OF THE UNIVERSITY OF CALIFORNIAInventors: Ming Chen Hammond, Alexander J. Westermann, Qian Qin
-
Patent number: 9637751Abstract: Polypeptide sequences for inducing recombinant protein bodies are described. The sequences comprise a polyproline II (PPII) structure and/or a proline-rich sequence between two cysteine residues on either end. Recombinant protein bodies are useful for protein production because they allow for simple and efficient purification of high quantities of recombinant protein. In addition, other methods of using recombinant protein bodies, for example, in vaccination and food products, are also described.Type: GrantFiled: May 31, 2011Date of Patent: May 2, 2017Assignee: ERA BIOTECH, S.A.Inventors: Maria Dolores Ludevid Múgica, Maria Immaculada Llop Tous, Pablo Marzábal Luna, Minu Joseph, Blanca Llompart Royo, Margarita Torrent Quetglas, Miriam Bastida Virgili
-
Patent number: 9637752Abstract: The present invention provides novel regulatory elements for use in plants. The present invention also provides DNA constructs containing these novel regulatory elements; transgenic cells, plants, and seeds containing these novel regulatory elements; and methods for preparing and using the same.Type: GrantFiled: September 13, 2013Date of Patent: May 2, 2017Assignee: Monsanto Technology LLCInventors: Stanislaw Flasinski, Charles R. Dietrich
-
Patent number: 9637753Abstract: The present invention encompasses a method for the production of Apolipoproteins in dimeric and/or oligomeric forms (comprising three or more units) of muteins of the human ApoA-1 protein in plant seeds, novel variants of these proteins having an increased stability and capacity to transport cholesterol compared to the native ApoA-1 protein, expression systems for the production thereof, plants transformed capable of producing said proteins in seeds in dimeric and/or oligomeric forms, and nutraceutical derivatives of these plants.Type: GrantFiled: December 19, 2006Date of Patent: May 2, 2017Assignee: PLANTECHNO S.R.L.Inventors: Corrado Fogher, Serena Reggi, Kiril Perfanov
-
Patent number: 9637754Abstract: Disclosed are mutant CDK inhibitor (CKI) polypeptides having dominant negative antagonist activity against wild-type CKI proteins, as well as related compositions, including nucleic acids and vectors encoding the mutant CKI polypeptides and transformed host cells and transgenic plants comprising such nucleic acids and vectors. Also disclosed are related methods for using the mutant proteins to modulate cell division in cells, particularly plant cells.Type: GrantFiled: April 15, 2014Date of Patent: May 2, 2017Assignee: Targeted Growth, Inc.Inventors: Paul Olivier, Jay Derocher, James Roberts
-
Patent number: 9637755Abstract: The invention relates to the field of plant improvement, in particular of the improvement of yield for plants, by using a transgene containing the BETL9 promoter driving expression of the MRP1 protein.Type: GrantFiled: October 22, 2013Date of Patent: May 2, 2017Assignee: BIOGEMMAInventors: Gregorio Hueros Soto, Wyatt Paul, Pascual Perez
-
Patent number: 9637756Abstract: The present invention relates to isolated nucleic acid molecules and their corresponding encoded polypeptides able confer the trait of improved plant size, vegetative growth, growth rate, seedling vigor and/or biomass in plants challenged with saline conditions. The present invention further relates to the use of these nucleic acid molecules and polypeptides in making transgenic plants, plant cells, plant materials or seeds of a plant having plant size, vegetative growth, growth rate, seedling vigor and/or biomass that are improved in saline conditions with respect to wild-type plants grown under similar conditions.Type: GrantFiled: October 29, 2012Date of Patent: May 2, 2017Assignee: Ceres, Inc.Inventors: Fasong Zhou, Kenneth A. Feldmann, Julissa Sosa
-
Patent number: 9637757Abstract: The present invention relates to a tomato plant (Solanum lycopersicum L.) which may comprise a genetic determinant that confers resistance to Pepino Mosaic Virus (PepMV), wherein the resistance is characterized by the presence of at least QTL1 and/or QTL2. The invention also relates to sources for obtaining said genetic determinant, representative seed of which were deposited with the NCIMB under accession numbers NCIMB 41927, NCIMB 41928, NCIMB 42068, and NCIMB 42069. The invention further relates to seeds and progeny of the plant and to its fruits and processed fruits. In addition the invention relates to molecular markers linked to PepMV resistance conferring QTLs and the use thereof.Type: GrantFiled: February 22, 2013Date of Patent: May 2, 2017Assignee: RIJK ZWAAN ZAADTEELT EN ZAADHANDEL B.V.Inventors: Arie Vogelaar, Evert Willem Gutteling, Dorthe Bettina Drager, Rudolf Verhoef, Zeger Otto Van Herwijnen
-
Patent number: 9637758Abstract: The present invention relates to a Sendai virus or recombinant Sendai virus vector. In particular the present invention provides methods, vectors, formulations, compositions, and kits for a modified Enders strain Sendai viral vector. An immunogenic vector can be used in any in vitro or in vivo system. Moreover, some embodiments include vectors for imaging virus growth, location and transmission.Type: GrantFiled: April 13, 2012Date of Patent: May 2, 2017Assignee: St. Jude Children's Research HospitalInventors: Julia Lea Hurwitz, Toru Takimoto, Charles John Russell, Allen Portner, Karen Slobod
-
Patent number: 9637760Abstract: This invention refers to a procedure for obtaining a biocrude from greenhouse gases, a procedure which is advantageous industrially and continuous. By means of said procedure it is possible to capture, convert and revalue CO2, among other greenhouse gases, in an efficient manner, in such a way that a net negative balance is obtained, which in other words means that with this procedure it is possible to capture more CO2 than is generated which makes it beneficial and sustainable in the environment.Type: GrantFiled: June 23, 2010Date of Patent: May 2, 2017Inventor: Bernard A. J. Stroïazzo Mougin
-
Patent number: 9637761Abstract: The present invention relates to a recombinant microorganism metabolizing 3,6-anhydro-L-galactose and a use thereof, and, more particularly, can produce ethanol from a recombinant microorganism expressing an enzyme group involved in a metabolic pathway of 3,6-AHG.Type: GrantFiled: May 7, 2014Date of Patent: May 2, 2017Assignee: Korea University Research and Business FoundationInventors: Kyoung Heon Kim, In-Geol Choi, Eun-Ju Yun, Sae Young Lee, Hee Taek Kim
-
Patent number: 9637762Abstract: A system and a process for the production of ethanol and related products from lignocellulosic biomasses (second generation—2G-ethanol), particularly from sugarcane bagasse and straw is provided, however not limited thereto, integrated with conventional processes for the production of ethanol (first generation—1G-ethanol) such as, for example, from sugarcane juice and/or molasses (either in sugar and ethanol plants or in autonomous distilleries), corn, grain, wheat, sugary sorghum, white beetroot, among others, including the recovery/reuse of streams and effluents. More specifically, an integrated process for the production of ethanol and related products where the process warrants increased efficiency particularly in the use of the raw material, steam, electric power and treated water is provided.Type: GrantFiled: March 27, 2013Date of Patent: May 2, 2017Assignee: CTC—CENTRO DE TECNOLOGIA CANAVIEIRA S.A.Inventors: José Ricardo Medeiros Pinto, Oswaldo Godoy Neto, Juliana Conceição Teodoro, Danilo Ribeiro De Lima, Liliane Pires Andrade, José Augusto Travassos Rios Tomé, Célia Maria Araújo Galvão
-
Patent number: 9637763Abstract: The invention relates to the production of aromatic molecules in prokaryotic and eukaryotic hosts such as E. coli, yeasts, filamentous fungi, algae, microalgae, other plant cells.Type: GrantFiled: June 22, 2012Date of Patent: May 2, 2017Assignee: RHO Renewables, Inc.Inventor: Philip J. Barr
-
Patent number: 9637764Abstract: This document describes biochemical pathways for producing pimelic acid, 7-aminoheptanoic acid, 7-hydroxyheptanoic acid, heptamethylenediamine or 1,7-heptanediol by forming two terminal functional groups, comprised of carboxyl, amine or hydroxyl group, in a C7 aliphatic backbone substrate. These pathways, metabolic engineering and cultivation strategies described herein rely on the carbon chain elongation enzymes or homologs thereof associated with the cyclohexane carboxylate biosynthesis from Syntrophus aciditrophicus or 2-aminoadipate lysine biosynthesis.Type: GrantFiled: December 23, 2013Date of Patent: May 2, 2017Assignee: INVISTA NORTH AMERICA S.A.R.L.Inventors: Adriana Leonora Botes, Alex Van Eck Conradie, Changlin Chen, Paul S. Pearlman
-
Patent number: 9637765Abstract: A process for pretreatment of a biomass material, comprising subjecting a biomass material to a biphasic mixture of water and supercritical CO2 at a temperature in the range of 150° C. to 250° C. under high pressure for a time of from 10 seconds to 100 minutes. In particular embodiments, the process is performed as a two-stage temperature process wherein an initial short high-temperature stage is conducted at a temperature of at least 200° C. for up to 20 minutes and a subsequent longer low-temperature stage is conducted at a temperature of at least 140° C. and up to 190° C. for 10-120 minutes.Type: GrantFiled: March 10, 2011Date of Patent: May 2, 2017Assignee: CORNELL UNIVERSITYInventors: Jeremy Luterbacher, Larry P. Walker, Jefferson W. Tester
-
Host cells and methods for producing 1-deoxyxylulose 5-phosphate (DXP) and/or a DXP derived compound
Patent number: 9637766Abstract: The present invention provides for a genetically modified host cell capable of producing 1-deoxyxylulose 5-phosphate or 1-deoxy-D-xylulose 5-phosphate (DXP) (12), and optionally one or more DXP derived compounds, comprising: (a) a mutant RibB, or functional variant thereof, capable of catalyzing xylulose 5-phoshpate and/or ribulose 5-phospate to DXP, or (b) a YajO, or functional variant thereof, and a XylB, or functional variant thereof.Type: GrantFiled: June 17, 2016Date of Patent: May 2, 2017Assignee: The Regents of the University of CaliforniaInventors: James Kirby, Jeffrey L. Fortman, Minobu Nishimoto, Jay D. Keasling -
Patent number: 9637767Abstract: Disclosed is a method for preparing a mixture of malto-oligosaccharides. Generally, a dry-milled corn fraction, such as a corn flour from which germ and fiber have been removed, is subjected to hydrolysis, typically catalyzed with acid or an enzyme such as an ?-amylase enzyme, under conditions suitable to form a mixture of malto-oligosaccharides. A gluten fraction is removed and the enzyme is inactivated, such as with heat. The mixture of malto-oligosaccharides then may be recovered from remaining solids and purified.Type: GrantFiled: August 14, 2015Date of Patent: May 2, 2017Assignee: Grain Processing CorporationInventors: Steven L. Ramsden, Zachary J. Halloran, Albert J. Pollmeier, Jeff M. Underwood
-
Patent number: 9637768Abstract: The present invention relates to a method for preparing a sialic acid derivative characterized by performing both of a process for preparing CMP-N-acetylneuraminic acid using N-acetyl-D-glucosamine and a process for preparing the sialic acid (neuraminic acid) derivative that combines a sialic acid with a galactose derivative or a lactose derivative, together, in one reactor. According to the method for preparing a sialic acid derivative of the present invention, expensive cytidine 5?-monophosphate (CMP) is capable of being recycled in a reactor, such that an amount of the CMP introduced into the reactor may be reduced, and the sialic acid derivative is capable of being prepared at a significantly high efficiency by using cheap N-acetyl-D-glucosamine, and pyruvate as substrates.Type: GrantFiled: May 30, 2014Date of Patent: May 2, 2017Assignee: GENECHEM INC.Inventors: Jin Suk Woo, Byung-Gee Kim, Dae Hee Kim, Yun Hee Choi, Jae-Kyung Song, Sun Youp Kang, Won Min Seo, Ji Young Yang, Sang Mi Lee
-
Patent number: 9637769Abstract: The present invention is related to a method to reduce peptide amidation activity in a given cell line, cell lines with reduced peptide amidation activity, and uses thereof.Type: GrantFiled: April 16, 2013Date of Patent: May 2, 2017Assignee: LEK PHARMACEUTICALS D.D.Inventors: Mihaela {hacek over (S)}kulj, Dominik Gaser
-
Patent number: 9637770Abstract: Provided is a biosensor including a scaffold and a dye complex, the dye complex including a clay material associated with a dye material, where the dye complex is at least partially embedded in the scaffold. Also provided is a process for preparing the biosensor, an apparatus including the biosensor and methods for detecting viable microorganisms in an aqueous fluid test sample making use of the biosensor, for predicting biofouling in a fluid flowing system and for determining a concentration of disinfectant required to disinfect an aqueous fluid.Type: GrantFiled: March 7, 2012Date of Patent: May 2, 2017Assignee: MEKOROT WATER COMPANY, LTD.Inventors: Eli Kotzer, Robert Armon
-
Patent number: 9637771Abstract: Particular aspects provide a method of sampling, testing and validating test lots (e.g., single-unit production lots), comprising: assembling a plurality of product portions from each of a plurality of test lots and combining the collected product portions to provide a corresponding set of test lot samples (wherein each test lot sample is attributed to a particular corresponding test lot); enriching the set of test lot samples; removing equal portions of each enriched sample, and combining the removed portions to provide a modular composite sample; and testing of the modular composite sample for the target agent/organism, wherein where such testing is positive, individual test lots may nonetheless yet be validated by further testing of a respective enriched test lot sample and obtaining a negative test result. The methods have broad utility for monitoring all sorts of test lots (e.g., environmental lots, production lots, pharmaceutical lots, etc.Type: GrantFiled: August 28, 2014Date of Patent: May 2, 2017Assignee: Institute for Environmental Health, Inc.Inventor: Mansour Samadpour
-
Patent number: 9637772Abstract: Organic-inorganic nanoflowers, methods of synthesis, and uses of the nanoflowers are described. It has been found that organic-inorganic nanoflowers can be grown in the presence of a solid substrate containing copper without the requirement for added copper ion. The method includes exposing bacteria to a solid substrate containing copper in the presence of an aqueous solution that contains phosphate ions. The aqueous solution can additionally contain chloride ions, similar to that of a phosphate-buffered saline composition. The solid substrate can be an alloy of copper and tin, and the substrate can have phosphorus incorporated into it.Type: GrantFiled: May 27, 2016Date of Patent: May 2, 2017Assignee: AEREUS TECHNOLOGIES INC.Inventors: Maurice Ringuette, Valerian Pershin, Heldder Gutierrez
-
Patent number: 9637773Abstract: Provided is a compound that comprises the structure: where SIG is a signaling molecule and R3 is a formyl, a succinyl, a methyl succinyl, or a myristoyl. Also provided is a kit is provided that comprises the above compound, with instructions for determining the presence of the enzyme. Additionally, a method is provided for determining whether a sample has an enzyme that removes a succinyl, a methyl succinyl, a formyl, or a myristoyl moiety from an ?-amino of a lysine. Also provided is a method of determining whether a molecule inhibits an enzyme that removes a succinyl, a methyl succinyl, a formyl, or a myristoyl moiety from an ?-amino of a lysine.Type: GrantFiled: January 13, 2011Date of Patent: May 2, 2017Assignee: Enzo Life Sciences, Inc.Inventors: Konrad T. Howitz, Zhongda Zhang, Anne Kisielewski, Elizabeth Dale, Wayne Forrest Patton
-
Patent number: 9637774Abstract: Compositions, devices, kits and methods are disclosed for assaying glucose with a glycosylated, modified flavin adenine dinucleotide-dependent glucose dehydrogenase (FAD-GDH), variant thereof or an active fragment thereof, where at least one asparagine residue at positions N2, N168 and N346 of mature, wild-type A. oryzae FAD-GDH according to SEQ ID NO:2 is substituted by one or more amino acids not suitable for glycosylation, thereby eliminating or inactivating, respectively, a potential glycosylation site at this position.Type: GrantFiled: October 30, 2014Date of Patent: May 2, 2017Assignee: Roche Diabetes Care, Inc.Inventors: Hartmut Duefel, Thomas Meier, Michael Tacke
-
Patent number: 9637775Abstract: A system and method for processing and detecting nucleic acids from a set of biological samples, comprising: a molecular diagnostic module configured to receive nucleic acids bound to magnetic beads, isolate nucleic acids, and analyze nucleic acids, comprising a cartridge receiving module, a heating/cooling subsystem and a magnet configured to facilitate isolation of nucleic acids, a valve actuation subsystem including an actuation substrate, and a set of pins interacting with the actuation substrate, and a spring plate configured to bias at least one pin in a configurations, the valve actuation subsystem configured to control fluid flow through a microfluidic cartridge for processing nucleic acids, and an optical subsystem for analysis of nucleic acids; and a fluid handling system configured to deliver samples and reagents to components of the system to facilitate molecular diagnostic protocols.Type: GrantFiled: March 28, 2014Date of Patent: May 2, 2017Assignee: NeuMoDx Molecular, Inc.Inventors: Michael T. Kusner, Jeffrey Williams
-
Patent number: 9637776Abstract: This disclosure features methods of identifying an organism. The methods include: (a) obtaining a nucleic acid sample from an organism; (b) imaging said nucleic acid; (c) obtaining a restriction map of said nucleic acid; and (d) correlating the restriction map of said nucleic acid with a restriction map database, thereby identifying the organism.Type: GrantFiled: May 14, 2008Date of Patent: May 2, 2017Assignee: OpGen, Inc.Inventor: Adam M. Briska
-
Patent number: 9637777Abstract: Disclosed are methods of multiplexed analysis of oligonucleotides in a sample, including a method of preventing a significant reduction in duplexes detectable in a hybridization assay involving (i) selecting probe lengths for sets of oligonucleotide probes, wherein probes include different subsequences such that at least one subsequence is complementary to a subsequence in a cognate target; wherein probes for longer cognate targets are longer in length than probes for shorter cognate targets, (ii) selecting, for each set of probes, a density of oligonucleotides probes attached per unit area on a solid phase carrier which is below a limit at which the significant reduction in detectable duplexes is predicated to take place, (iii) producing the probes and affixing them to different solid phase carriers at the selected density, and (iv) annealing targets to the probes, wherein signal intensities of probes and targets of different lengths are about the same.Type: GrantFiled: April 21, 2014Date of Patent: May 2, 2017Assignee: BioArray Solutions, Ltd.Inventors: Michael Seul, Sukanta Banerjee, Jiacheng Yang, Tatiana Vener
-
Patent number: 9637778Abstract: This invention provides novel compositions and processes for analyte detection, quantification and amplification. Nucleic acid arrays and libraries of analytes are usefully incorporated into such compositions and processes. Universal detection elements, signaling entities and the like are employed to detect and if necessary or desirable, to quantify analytes. Amplification of target analytes are also provided by the compositions and processes of this invention.Type: GrantFiled: July 29, 2015Date of Patent: May 2, 2017Assignee: Enzo Biochem, Inc.Inventors: Elazar Rabbani, Jannis G. Stavrianopoulos, James J. Donegan, Jack Coleman
-
Patent number: 9637779Abstract: Transcription in mammalian cells can be assessed at a genome-wide level, but it has been difficult to reliably determine whether individual transcripts are derived from the Plus- or Minus-strands of chromosomes. This distinction can be critical for understanding the relationship between known transcripts (sense) and the complementary antisense transcripts that may regulate them. Here we describe a technique that can be used to (i) identify the DNA strand of origin for any particular RNA transcript and (ii) quantify the number of sense and antisense transcripts from expressed genes at a global level. We examined five different human cell types and in each case found evidence for antisense transcripts in 2900 to 6400 human genes. The distribution of antisense transcripts was distinct from that of sense transcripts, was non-random across the genome, and differed among cell types.Type: GrantFiled: December 2, 2009Date of Patent: May 2, 2017Assignee: The Johns Hopkins UniversityInventors: Bert Vogelstein, Kenneth W. Kinzler, Yiping He, Victor Velculescu, Nickolas Papadopoulos
-
Patent number: 9637780Abstract: A hot start enzyme composition is described that includes a hot start nuclease, a nucleic acid polymerase, and a substantially double-stranded oligonucleotide that inhibits the catalytic activity of the nucleic acid polymerase at temperatures lower than the melting temperature of the oligonucleotide.Type: GrantFiled: June 11, 2013Date of Patent: May 2, 2017Assignee: Hanwha Techwin Co., Ltd.Inventors: Win D. Cheung, Jason A. Opdyke
-
Patent number: 9637781Abstract: The present invention provides a PCR-free, multiplexed ligation assay for miRNA expression analysis that produces highly quantitative, 10-100 plex miRNA profiling in a single reaction. The inventive methods use a 2-step ligation assay to generate an array of miRNA specific ligation products that can be decoded and quantified by a size discrimination method such as gel electrophoresis or single molecule separation. One embodiment is a low-cost assay that can be performed using standard tools available in nearly all molecular biology laboratories. This assay requires nothing more than a gel apparatus and reader for detection. Other embodiments include use of magnetic beads and other size exclusion apparatus which give increasingly higher sensitivity, lower sample consumption, and reduced processing steps.Type: GrantFiled: February 13, 2013Date of Patent: May 2, 2017Assignee: The Johns Hopkins UniversityInventors: Tza-Huei Wang, Kelvin J. Liu, Yunke Song
-
Patent number: 9637782Abstract: Mitigation of photo-induced damage in excitation illuminated reactions and analyses utilizing such reactions results in an enhanced performance for the reactions and the analyses. There is provided a novel class of triplet-state quenchers for mitigating photo-induced damage which are both simple in structure and effective at preventing and/or reducing photo-induced damage to reaction components of excitation illuminated reaction mixtures. Also provided are methods of using the compounds of the invention, devices and kits incorporating the compounds of the invention.Type: GrantFiled: September 27, 2013Date of Patent: May 2, 2017Assignee: Pacific Biosciences of California, Inc.Inventors: Gene Shen, Andrei Fedorov, Wei-Chuan Sun, Stephen Yue
-
Patent number: 9637783Abstract: This invention relates to processes that amplify, in a polymerase chain reaction architecture, oligonucleotide analogs that incorporate non-standard nucleobase analogs from an artificially expanded genetic information system. These pair in DNA duplexes via patterns of hydrogen bonds that are different from patterns that join the thymine-adenine and guanine-cytosine nucleobase pairs.Type: GrantFiled: June 22, 2015Date of Patent: May 2, 2017Inventors: Steven Benner, Roberto Laos, Nicole Leal, Nilesh Karalkar, Zunyi Yang
-
Patent number: 9637784Abstract: The invention provides methods and kits for ordering sequence information derived from one or more target polynucleotides. In one aspect, one or more tiers or levels of fragmentation and aliquoting are generated, after which sequence information is obtained from fragments in a final level or tier. Each fragment in such final tier is from a particular aliquot, which, in turn, is from a particular aliquot of a prior tier, and so on. For every fragment of an aliquot in the final tier, the aliquots from which it was derived at every prior tier is known, or can be discerned. Thus, identical sequences from overlapping fragments from different aliquots can be distinguished and grouped as being derived from the same or different fragments from prior tiers. When the fragments in the final tier are sequenced, overlapping sequence regions of fragments in different aliquots are used to register the fragments so that non-overlapping regions are ordered.Type: GrantFiled: August 20, 2013Date of Patent: May 2, 2017Assignee: Complete Genomics, Inc.Inventor: Radoje Drmanac
-
Patent number: 9637785Abstract: The invention provides methods and kits for ordering sequence information derived from one or more target polynucleotides. In one aspect, one or more tiers or levels of fragmentation and aliquoting are generated, after which sequence information is obtained from fragments in a final level or tier. Each fragment in such final tier is from a particular aliquot, which, in turn, is from a particular aliquot of a prior tier, and so on. For every fragment of an aliquot in the final tier, the aliquots from which it was derived at every prior tier is known, or can be discerned. Thus, identical sequences from overlapping fragments from different aliquots can be distinguished and grouped as being derived from the same or different fragments from prior tiers. When the fragments in the final tier are sequenced, overlapping sequence regions of fragments in different aliquots are used to register the fragments so that non-overlapping regions are ordered.Type: GrantFiled: August 20, 2013Date of Patent: May 2, 2017Assignee: Complete Genomics, Inc.Inventor: Radoje Drmanac
-
Patent number: 9637786Abstract: The invention relates to methods for pairwise sequencing of a double-stranded polynucleotide template, which permit the sequential determination of nucleotide sequences in two distinct and separate regions on complementary strands of the double-stranded polynucleotide template. The two regions for sequence determination may or may not be complementary to each other.Type: GrantFiled: February 17, 2016Date of Patent: May 2, 2017Assignee: Illumina Cambridge LimitedInventor: Geoffrey Paul Smith
-
Patent number: 9637787Abstract: A 3D organotypic culture which phenocopies aggressive, invasive cancer and methods of use thereof are provided.Type: GrantFiled: September 4, 2014Date of Patent: May 2, 2017Assignee: The Trustees of the University of PennsylvaniaInventor: Anil K. Rustgi
-
Patent number: 9637788Abstract: The present invention provides a method for detecting the presence or absence of, or for discriminating between, blood type variants, including RHD*r?s, RHD*DIIIa, RHD*DIVa-2, RHCE*css and RHCE*ce733G. The method comprises genotyping a sample obtained from a human subject at one or more positions in intron 7 of the RHD gene and/or in intron 7 of the RHCE gene. The invention also provides products, in particular, probes, primers and kits for use in the method of the invention.Type: GrantFiled: June 14, 2012Date of Patent: May 2, 2017Assignee: Progenika Biopharma, S.A.Inventors: Jorge Ochoa, Monica Lopez, Sergio Escorza, Diego Tejedor, Antonio Martinez, Laureano Simon
-
Patent number: 9637789Abstract: The present invention provides methods for predicting the risk of an individual developing antibodies to factor VIII by identifying a single nucleotide polymorphism of an immune response or immune modifier gene. The invention further provides oligonucleotides, diagnostic kits, microarrays, and isolated nucleic acids comprising single nucleotide polymorphisms of immune response or immune modifier genes.Type: GrantFiled: April 21, 2011Date of Patent: May 2, 2017Assignee: Jan Astermark and Erik BerntorpInventors: Jan Astermark, Erik Berntorp
-
Patent number: 9637790Abstract: Provided herein are methods for detecting mutations in nucleic acid, and compositions and kits for performing such methods. In particular, nucleic acid amplification and fluorescence detection methods are provided to detect mutations and assess the mutational load. The methods are based on a set of adjacently binding probes wherein one probe is labelled with a quencher and the other is a self-indicating probe labelled with fluorophore and quencher. The binding of the probes is analyzed by melting curve analysis.Type: GrantFiled: December 1, 2011Date of Patent: May 2, 2017Assignee: Brandeis UniversityInventors: Adam E. Osborne, Lawrence J. Wangh, John E. Rice