Abstract: A direct-foam cleaning product comprising a foam having a compression force of about 2.4 gf*mm to about 4.3 gf*mm. Such direct-foam cleaning product provides good foaming properties and surface coverage when the composition is sprayed directly onto soiled dishware. This leads to efficient cleaning of soiled dishes via a direct-foam and rinse action, which avoids traditional methods of soaking soiled dishes in detergent baths and/or scrubbing soiled dishware with a sponge or cleaning implement.

Abstract: A method of cleaning dishware with a direct-foam cleaning product is provided. The product comprises a foam having a compression force of about 2.4 gf*mm to about 4.3 gf*mm. Such direct-foam cleaning product provides good foaming properties and surface coverage when the composition is sprayed directly onto soiled dishware. This leads to efficient cleaning of soiled dishes via a direct-foam and rinse action, which avoids traditional methods of soaking soiled dishes in detergent baths and/or scrubbing soiled dishware with a sponge or cleaning implement.

Abstract: A cleaning product including a spray dispenser and a cleaning composition suitable for spraying and foaming, the composition housed in the spray dispenser wherein the composition includes: i) from about 5 to about 15% by weight of the composition of a surfactant system; and ii) a glycol ether solvent selected from the group consisting of glycol ethers of Formula I: R1O(R2O)nR3, Formula II: R4O(R5O)nR6 and mixtures thereof wherein R1 is a linear or branched C4, C5 or C6 alkyl or a substituted or unsubstituted phenyl, R2 is ethyl or isopropyl, R3 is hydrogen or methyl and n is 1, 2 or 3 R4 is n-propyl or isopropyl, R5 is isopropyl, R6 is hydrogen or methyl and n is 1, 2 or 3 and a specific cyclic diamine.

Abstract: Caustic-free detergent compositions are provided. Detergent compositions including an aminocarboxylate, water conditioning agent, non-caustic source of alkalinity and water beneficially do not require the use of additional surfactants and/or polymers to provide suitable detergency and prevent scale build-up on treated surfaces. The detergent compositions are used with a sanitizer to employ the caustic-free detergent compositions are particularly suitable for use as low temperature ware wash detergents that beneficially reduce scale build-up. Methods of employing the caustic-free detergent compositions are also provided.

Abstract: Objects of the present invention are to provide a beer-taste beverage excellent in “umami (taste)”, refreshing feeling and “kire (sharpness)”, a method for producing the same, and a method for improving taste of the same. In the beer-taste beverage according to the present invention, use ratio of malt is at least 50%, alcohol content is at least 4%, bitterness value is at least 10, and total polyphenol is 140 ppm or less. The method for producing the beer-taste beverage includes a fermentation step of obtaining fermentation liquid by alcohol fermentation, and a post-fermentation step after alcohol fermentation, wherein in the post-fermentation step, fermentation liquid obtained in the fermentation step is treated with polyvinylpolypyrrolidone, to reduce the total polyphenol to 140 ppm or less.

Abstract: Systems and methods for improved flow properties in fluidic and microfluidic systems are disclosed. The system includes a microfluidic device having a first microchannel, a fluid reservoir having a working fluid and a pressurized gas, a pump in communication with the fluid reservoir to maintain a desired pressure of the pressurized gas, and a fluid-resistance element located within a fluid path between the fluid reservoir and the first microchannel. The fluid-resistance element includes a first fluidic resistance that is substantially larger than a second fluidic resistance associated with the first microchannel.

Abstract: Systems and methods for improved flow properties in fluidic and microfluidic systems are disclosed. The system includes a microfluidic device having a first microchannel, a fluid reservoir having a working fluid and a pressurized gas, a pump in communication with the fluid reservoir to maintain a desired pressure of the pressurized gas, and a fluid-resistance element located within a fluid path between the fluid reservoir and the first microchannel. The fluid-resistance element includes a first fluidic resistance that is substantially larger than a second fluidic resistance associated with the first microchannel.

Abstract: A blade tip-provided micropipette holding apparatus has a tubular micropipette holder so constructed that a blade tip-provided micropipette to be inserted into a living cell can be mounted on a front-end portion thereof and a holder-holding device for holding the micropipette holder. The holder-holding device has a base member and a tubular rotating member which is rotatably held by the base member and into which the tubular micropipette holder can be penetrated. The tubular rotating member has holder position adjusting mechanisms each composed of a plurality of side holes formed on a side surface of the tubular rotating member at different positions of the side surface thereof in a circumferential direction thereof and holder position adjusting members each capable of entering into the tubular rotating member from the side holes and contacting an outer surface of the micropipette holder penetrating through the tubular rotating member.

Abstract: Device for treating at least one biological cell comprising: at least one treatment support comprising a receiving surface enabling the adhesion of the at least one biological cell, at least one actuator capable of deforming said support in order to apply a stress to said at least one cell, and means of controlling said at least one actuator such that the actuator deforms the treatment support according to a given amplitude of deformation and for a given duration so as to generate at least one transitory pore in a membrane of the biological cell.

Abstract: The invention relates to an incubator (10) for creating and maintaining a microclimate with controlled air humidity and temperature conditions. The incubator has a tray opening (12) for inserting trays (16) into an internal space which is bounded by walls (14a, 14b, 14c, 14d), as well as telescopic rails (20) having a first rail part (34) which can be connected to a side wall (14a, 14b) and a further second rail part (35) which can be connected to the tray (16). The invention is characterized in that the telescopic rails (20) are formed as components separately from the internal space of the incubator and from the trays (16), in that supports (22, 28) for the telescopic rails (20) are provided on the side walls (14), and in that the trays (16) can be detached from the telescopic rails (20) and the telescopic rails (20) can be detached from the supports (22, 28).

Abstract: Systems and methods for improved flow properties in fluidic and microfluidic systems are disclosed. The system includes a microfluidic device having a first microchannel, a fluid reservoir having a working fluid and a pressurized gas, a pump in communication with the fluid reservoir to maintain a desired pressure of the pressurized gas, and a fluid-resistance element located within a fluid path between the fluid reservoir and the first microchannel. The fluid-resistance element includes a first fluidic resistance that is substantially larger than a second fluidic resistance associated with the first microchannel.

Abstract: A cell separation device imparts vibration to a cell culture film having a front surface to which cells adhere, from a rear surface side of the film, and consequently separates the cells from the film. The device includes an ultrasonic probe having an ultrasonically-vibrating vibrator, a frame disposed to surround the vibrator and a first detection part configured to detect a force applied to the frame in a specified direction. The vibrator selectively imparts ultrasonic vibration to specified cells adhering to the front surface of the film. Based on a detection result obtained by the first detection part, the frame is pushed until the force applied from the rear surface of the film to the frame reaches a specified magnitude. Thereafter, the vibrator ultrasonically vibrates in a state in which the frame makes contact with the rear surface of the film.

Abstract: A sorting device is provided. The sorting device includes: a carrier substrate; an input unit disposed on the carrier substrate for inputting a biological sample into the sorting device; a porous material disposed on the carrier substrate and adjacent to the input unit, wherein the porous material contains antigen molecules having specificity to a target biological analyte; a driving module generating at least one driving force in the porous material so as to sort the biological sample based on the affinity for the antigen and the driving force; and an output unit disposed on the carrier substrate and adjacent to the porous material for collecting the sorted target biological analyte. A sorting method is also provided.

Abstract: A device for preparing adipose tissue for transplantation, from lobular fat extracted, for instance, by liposuction. The device comprises at least one washing and separating container (1) having a washing chamber (11) for washing the liposuctioned material, which washing and separating container (1) has at least one inlet (12) and at least one outlet (13) for the liposuctioned material to enter the washing chamber (11) through the inlet (12) and for at least part of said material, the fluid component, to exit said chamber 11 through the outlet 13. The washing and separating container (1) is coupled to stirring means via coupling means (2) for releasably coupling said washing and separating container (1) to said stirring means, drive means being provided for driving said stirring means.

Abstract: Cells in a test liquid are captured and observed easily and accurately. A cell capture filter 30 is fixed by a first protrusion part 125 of a lid member 100 and a second protrusion part 225 of a housing member 200 in a cell capture device 1, and a first fitting part 130 and a second fitting part 230 are fitted with each other. This allows the cell capture filter 30 to be fixed between the lid member 100 and the housing member 200 in a state where a filter region of the cell capture filter 30 is surrounded by the looped first protrusion part 125 and the looped second protrusion part 225.

Abstract: A method of precipitating cell membrane fragments from a cell lysate is disclosed. The method comprises contacting the cell lysate with a hydrophobic chelator and a metal ion under conditions that allow precipitation of the cell membrane fragments. Kits for precipitating cell membrane fragments are also disclosed.

Abstract: A magnetic separation system configured to separate with high qualitative and quantitative yield magnetized cells from cell mixtures, comprising at least one electromagnet structured to generate a magnetic field flux about a plurality of separation zones and sufficient to attract a majority of the magnetized cells in the mixture, and a pump to drive the cell mixture at a controlled flow rate through a tube disposed within the zones thereby separating a majority of the magnetized cells from the mixture. The system is particularly useful to retrieve rare cells from a fluid mixture of cells having low abundance of the rare cells relative to the rest of the cells while sustaining viability of the cells.

Abstract: The invention pertains to a method to purify coccidial oocysts having dimensions between Dmin and Dmax from faeces comprising the steps of collecting the faeces containing the coccidial oocysts from host animals, diluting the faeces in an aqueous medium, separating a coarse fraction comprising macroscopic particulate matter from the diluted faeces and collecting an aqueous fraction containing the oocysts, characterised in that the method further comprises sieving the aqueous fraction over a first sieve deck having mesh openings to let the oocysts pass, to obtain an aqueous filtrate comprising the oocysts and a first residue comprising particles larger than the oocysts,and sieving the aqueous filtrate over a second sieve deck having mesh openings to obstruct passing of the oocysts through this sieve deck, to obtain a second residue comprising the purified oocysts and a waist filtrate comprising particles smaller than the oocysts.

Abstract: A stable emulsion, comprising oil, water and a stabilizer, wherein said stabilizer is selected from a single-cell microorganism.

Abstract: The invention is in the field of growth of Mycobacteria. In particular, agents have been identified which enhance the growth of Mycobacterial species, which are naturally slow-growing. Such agents can therefore be used in the identification of Mycobacteria and in the diagnosis of Mycobacterial infections.

Abstract: Compositions for and methods of manufacturing a fucosylated cell population are provided. The method may include expansion of the cells and/or cryopreservation of the cells under conditions that retain optimum levels of cell surface fucosylation.

Abstract: The present invention relates to a method for producing a cell mass structure, which produces a cell mass structure (adhesion pad 4) in which a plurality of cell aggregates 2 arranged inside a culture container 3 accommodating a culture liquid are fused to each other by being cultured. A plurality of pins 16 are erected inside the above-described culture container 3, the above-described cell aggregates 2 are accommodated by the plurality of pins 16 with parts between the plurality of pins 16 and pins 16 as accommodating portions H, and the cell aggregates 2 are cultured in a state of being accommodated in the plurality of adjacent accommodating portions H. It is possible to obtain a required shape and dimension, and the cell mass structure can be obtained without giving damage to cells further.

Abstract: Aspects of the present disclosure include methods for separating components having different densities from a biological sample droplet. Methods according to certain embodiments include contacting a surface of a support with a biological sample droplet that includes components of different densities; subjecting the biological sample droplet to a gravitational force to produce two or more regions in the biological sample droplet on the support surface, where each region in the biological sample droplet includes a component from the biological sample droplet having a different density; separating the biological sample droplet into two or more product droplets, wherein each product droplet includes a different region of the biological sample droplet; and collecting the one or more product droplets. Systems for practicing the subject methods are also described. Computer systems and kits are also provided.

Abstract: The invention provides methods, compositions and kits for the identification and enrichment of progenitor cell lines obtained from pluripotent stem cells.

Abstract: The invention provides methods and compositions relating to differentiated cell populations that derive from pluripotent stem cells. The methods relate to reducing the number of residual stem cells present in such populations. The compositions include differentiated cell populations that contain reduced number of stem cells or that contain no stem cells. Pluripotent stem cells may be reduced in number and/or function through exposure to low oxygen levels.

Abstract: Provided herein are methods and compositions for maintaining human stem cells in either a nave state or a primed state, inducing, promoting, inhibiting, or controlling the transition from one state to another, and detecting the state of a stem cell. Also disclosed are compositions comprising substantially homogenous populations of primed state or nave state stem cells.

Abstract: The present invention is a method that can promote the differentiation of cultured cells, and a cultured cell differentiation promoter that may suitably be used for the method. The differentiation of cultured cells is induced by bringing an alicyclic structure-containing polymer formed article into contact with cells that are being cultured.

Abstract: The present invention provides a method for preparing a cell culture medium comprising a mixture of blood products from two or more donors, comprising the steps of: a) providing at least a first blood product from a first donor; b) measuring the concentration of at least one quality factor in the first blood product; c) comparing the measured concentration of a quality factor to a concentration range predefined for the quality factor; d) selecting the first blood product for the cell culture medium if the concentration measured for the quality factor is in the predefined range and optionally converting the first selected blood product into a first processed blood product or else unselecting the first blood product.

Abstract: Methods for generating enucleated erythroid cells using pluripotent stem cells are provided. The methods permit the production of large numbers of cells. The cells obtained by the methods disclosed may be used for a variety of research, clinical, and therapeutic applications. Methods for generating megakaryocyte and platelets are also provided.

Abstract: Methods for obtaining purified populations of megakaryocytes and platelets by ex vivo culture of stem cells are provided herein.

Abstract: Methods for enhancing and disrupting activity of the aryl hydrocarbon receptor (AHR) and cells having disrupted or enhanced AHR activity. The methods and cells may be used to enhance production of specific cell populations during hemato-endothelial cell or hemato-lymphoid cell development.

Abstract: Human progenitor T cells that are able to successfully engraft a murine thymus and differentiate into mature human T and NK cells are described. The human progenitor T cells have the phenotype CD34+CD7+CD 1a?CD5? or CD34+CD7+CD1a?CD5+ and are derived from human hematopoietic stem cells, embryonic stem cells and induced pluripotent stem cells by coculture with cells expressing a Notch receptor ligand (OP9-DL1 or OP9-DL4). Such cells are useful in a variety of applications including immune reconstitution, the treatment of immunodeficiencies and as carriers for genes used in gene therapy.

Abstract: The invention relates to exosomes derived from mesenchymal stem cells and well as isolated populations of said exosomes and method for preparing said isolated exosome populations. The invention also relates to a pharmaceutical composition comprising said exosome or isolated exosome population and their use in a method of treating an immune-mediated inflammatory disease in a subject.

Abstract: Methods for producing hepatocytes from pluripotent human stem cells are disclosed herein. The stem cells are plated on a cell culture substrate comprising two laminins. The stem cells are then exposed to different cell culture mediums to induce differentiation. The resulting hepatocytes have higher metabolic capacity compared to hepatocytes cultured on different substrates.

Abstract: The present invention relates to a method for reprogramming a first cell type to an intermediate cell of a second cell type comprising the step of contacting the first cell with a first agent to modulate an integrin profile in the first cell type to provide an intermediate cell of the second cell type. The present invention also relates to a reprogrammed cell obtained by the method of the invention, a kit for reprogramming a first cell type to a second cell type as well as methods for treating a patient in need of cell based therapy, tissue replacement and cancer therapy.

Abstract: Disclosed herein are methods and systems for rapid detection of microorganisms in a sample. A genetically modified bacteriophage is also disclosed which comprises an indicator gene in the late gene region. The specificity of the bacteriophage, such as CBA120, allows detection of a specific microorganism, such as E. coli O157:H7, and an indicator signal may be amplified to optimize assay sensitivity.

Abstract: The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds.

Abstract: The present application relates to laccase variants and uses thereof as eco-friendly biocatalysts in various industrial processes. More in particular, the application relates to a polypeptide with laccase activity comprising an amino acid sequence that is at least 60% identical to the amino acid sequence according to SEQ ID NO: 1, wherein the polypeptide comprises an alanine residue at a position corresponding to amino acid 260 of SEQ ID NO: 1.

Abstract: Described herein are methods of inserting nucleic acid sequences into host cells. Also described herein are genetically stable host cells comprising inserted nucleic acid sequences and methods of using such host cells in the generation of proteins.

Abstract: The present invention concerns methods and compositions for stably tethered structures of defined compositions, which may have multiple functionalities and/or binding specificities. Particular embodiments concern homodimers comprising monomers that contain a dimerization and docking domain attached to a precursor. The precursors may be virtually any molecule or structure, such as antibodies, antibody fragments, antibody analogs or mimetics, aptamers, binding peptides, fragments of binding proteins, known ligands for proteins or other molecules, enzymes, detectable labels or tags, therapeutic agents, toxins, pharmaceuticals, cytokines, interleukins, interferons, radioisotopes, proteins, peptides, peptide mimetics, polynucleotides, RNAi, oligosaccharides, natural or synthetic polymeric substances, nanoparticles, quantum dots, organic or inorganic compounds, etc. Other embodiments concern tetramers comprising a first and second homodimer, which may be identical or different.

Abstract: Some aspects of this disclosure provide strategies, systems, reagents, methods, and kits that are useful for the targeted editing of nucleic acids, including editing a single site within the genome of a cell or subject, e.g., within the human genome. In some embodiments, fusion proteins of Cas9 and nucleic acid editing proteins or protein domains, e.g., deaminase domains, are provided. In some embodiments, methods for targeted nucleic acid editing are provided. In some embodiments, reagents and kits for the generation of targeted nucleic acid editing proteins, e.g., fusion proteins of Cas9 and nucleic acid editing proteins or domains, are provided.

Abstract: The present disclosure provides DNA-guided CRISPR systems; polynucleotides comprising DNA, RNA and mixtures thereof for use with CRISPR systems; and methods of use involving such polynucleotides and DNA-guided CRISPR systems.

Abstract: The present invention relates to variants having alpha-amylase activity and polynucleotides encoding the variants. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides, compositions comprising the variants, as well as methods of producing and using the variants.

Abstract: The present invention relates to oxidation stable alpha-amylase variants. The present invention also relates to poly-nucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.

Abstract: The present invention relates to a method for stabilising an aqueous solution of ?-amylase, in particular by the use of glycerol, potassium sorbate and sodium carbonate. The cocktail of additives is particularly effective at maintaining the enzymatic activity of ?-amylase over time. Another aim of the present invention consists of using the cocktail for the specific function of maintaining the enzymatic activity of the ?-amylase. Another aim of the present invention is to provide an aqueous solution of ?-amylase containing the aforementioned cocktail. A final aim of the present invention consists of using the ?-amylase aqueous solution in bread-making, in malting, as a food additive, as a digestive agent, for sweetener production, in pharmacy and, finally, for maltose and maltose-enriched syrup production.

Abstract: The present invention relates to Acanthophysium sp. KMF001 strain having high cellulose activity, and more particularly, to a novel Acanthophysium sp. KMF001 strain having the ability to produce endo-?-1,4-glucanase, ?-glucosidase, and cellobiohydrolase and to a culture of the strain. The novel Acanthophysium sp. KMF001 strain according to the present invention can produce highly active cellulases, including endo-?-1,4-glucanase, ?-glucosidase, and cellobiohydrolase, and thus can be advantageously used not only for enzymatic saccharification of lignocellulosic biomass, but also in various industrial fields, including the pulp and paper making industry, the detergent industry, the agricultural product processing industry, the fiber industry, and the livestock industry, in which the degradation of cellulose is required.

Abstract: A pectinase having improved thermostability is disclosed. The pectinase has a modified amino acid sequence of SEQ ID NO: 2, wherein the modification is a substitution of serine at position 181 with phenylalanine, methionine or leucine.

Abstract: The present invention relates to an improved method for preparing hydrogels, such as alginate hydrogels. The hydrogels of the present invention is in particular useful for immobilisation and preservation of biological material, such as cellular material, e.g. spermatozoa.

Abstract: An approach for preparing a vaccine using ultraviolet radiation is described. Aspects of this approach involve multiple iterations of inactivation of the vaccine using an ultraviolet radiation source at a set of different wavelengths and dosages. A recognition test of the vaccine using the set of different wavelengths and dosages is performed after the multiple iterations of inactivation. A controller compares results from the inactivation test and the recognition test to determine an area of acceptable radiation dosages and wavelengths generated from the ultraviolet radiation source that irradiate the live organisms without affecting efficacy and safety of the vaccine. The area of acceptable ultraviolet radiation dosages and wavelengths is representative of a difference between an ultraviolet radiation dosage that is required for inactivation and an ultraviolet radiation dosage that leads to a loss of recognition.

Abstract: The subject innovation relates to a RNA binding buffer comprising (a) at least one chaotropic agent; and (b) an organic solvent selected from the group consisting of ethylene carbonate, ethylene glycol diacetate and 2-pyrrolidone or combinations thereof. The subject innovation further relates to a method of binding RNA to a solid support and to a method of isolating RNA both making use of the binding buffer of the subject innovation. The subject innovation finally relates to a kit comprising the RNA binding buffer or the organic solvent as relevant substance therein.