Abstract: A method for measuring concentration of dehydrogenase and substrate thereof in the presence of living cells, wherein an electron mediator that is hard to be reduced by living cells, for example, a compound represented by Formula (I) shown below, an electron mediator represented by the Formula (I) shown below and a measurement reagent for dehydrogenase and substrate thereof comprising the electron mediator are disclosed. A?L?M ??(I) [In the Formula (I), A represents a structural part containing at least one anionic group, M represents a structural part for mediating electron transfer and L represents a linker part for linking the A of the M.

Abstract: Embodiments of the present disclosure relate to an electrochemical test strip comprising a reagent system for assaying activity of an enzyme that may be present is a sample. The present disclosure also provides a meter with a port for receiving the test strip and electronics for determining activity of the enzyme, if present, in the sample. Method for using the test strip for determining activity of the enzyme is also described.

Abstract: Coelenterazine analogs having luminescence properties different from those of known coelenterazine analogs are desired for various luciferases. The invention provides compounds represented by general formula (1) below.

Abstract: The methods described herein, referred to as PCR-Activated Sorting (PAS), allow nucleic acids contained in biological systems to be sorted based on their sequence as detected with nucleic acid amplification techniques, e.g., PCR. The nucleic acids can be free floating or contained within living or nonliving structures, including particles, viruses, and cells. The nucleic acids can include, e.g., DNA or RNA. Systems and devices for use in practicing methods of the invention are also provided.

Abstract: Provided herein is technology relating to the amplification-based detection of bisulfite-treated DNAs and particularly, but not exclusively, to methods and compositions for multiplex amplification of low-level sample DNA prior to further characterization of the sample DNA. The technology further provides methods for isolating DNA from blood or blood product samples, e.g., plasma samples.

Abstract: The present disclosure relates to devices and systems for separating motile pathogenic bacterial cells from samples. The disclosure also provides for methods of determining whether a sample is contaminated by pathogenic bacteria. The devices and systems disclosed herein are useful for screening water sources, environmental testing sites, food sources, and bodily fluids for the presence, absence, or quantity of bacterial cells in a sample representative of the screened sources.

Abstract: A method for detecting and characterizing a microorganism in a clinical sample includes introducing a clinical sample to a first culture vessel containing the culture medium; removing a test aliquot; separating DNA from the test aliquot; and performing nucleic acid tests on the DNA to identify the microorganism and to detect the presence or absence of one or more genetic antimicrobial resistance markers in the microorganism. If a microorganism is identified, an antimicrobial susceptibility test is performed wherein microbial growth in the antimicrobial susceptibility test is monitored by accessing growth or markers for growth and wherein the type and concentration of antimicrobial agents used in the antimicrobial susceptibility test is determined by the identity of the microorganism and the antimicrobial resistance markers detected. A device for performing the method is also provided.

Abstract: The object of the present invention is to provide a novel primer for use in the detection of Mycobacterium avium (M. avium), and a method for detection of M. avium conveniently, rapidly and with high precision by using the primer. More specifically, disclosed are an oligonucleotide which comprises a part or an entire of the nucleotide sequence selected from SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:37, SEQ ID NO:38, SEQ ID NO:39, SEQ ID NO:40, SEQ ID NO:41, SEQ ID NO:42, SEQ ID NO:130, SEQ ID NO:131, SEQ ID NO:132, SEQ ID NO:133, SEQ ID NO:134, SEQ ID NO:135 and SEQ ID NO:136, or a part or an entire of the sequence complementary to the nucleotide sequence, and which is capable of hybridizing with the nucleotide sequence for a Mycobacterium avium gene; a primer and a probe for use in the detection of M. avium, which comprises the oligonucleoride; and a method for detection of M. avium using the primer and/or probe.

Abstract: The invention concerns a system, device, kit and method for detecting the presence, or concentration of a target in a sample. An assay set comprising at least two spaced apart electrodes is used, comprising a recognition moiety, capable of specific binding to the target, which is attached to at least one of the electrodes or the substrate therein between. If the recognition moiety binds the target then a conductive bridge can be formed between the electrodes, based on the complex between the recognition moiety and the target. The conductive bridge is formed by using nucleation-center forming entities attached to the complexes or to the targets from which a conductive substance is substantially grown. Alternatively the conducting bridge forms a conductive polymer between the electrodes.

Abstract: Disclosed herein are compositions and methods for the processing, amplification and detection of polynucleotides using target-specific oligonucleotides (TSOs). Hybridization of TSOs to target polynucleotides guides target processing into and purification of small target fragments that then can be amplified and detected with high sensitivity and reproducibility. The method is specifically beneficial for highly degraded polynucleotides found in biological samples.

Abstract: Disclosed herein are data processing and calculating annotation systems and devices, and corresponding methods, for nucleic acid analysis. In particular, disclosed herein are methods for sizing a repeat region of a nucleic acid sample. For example, the methods disclosed herein use a ladder of amplification products to determine nucleic acid size.

Abstract: Methods, devices, and systems for performing intermittent detection during analytical reactions are provided. Such methods facilitate collection of reaction data from disparate reaction times. Further, such methods are useful for reducing photo-induced damage of one or more reactants in an illuminated analytical reaction at a given reaction time. In preferred embodiments, the reaction mixture is subjected to at least one illuminated and non-illuminated period and allowed to proceed such that the time in which the reaction mixture is illuminated is less than a photo-induced damage threshold period.

Abstract: The present disclosure relates to a primer set for preparation of a library used for next generation sequencing (NGS), and a method and a kit for making an NGS library using the same. The method for making the next generation sequencing (NGS) library using the primer set according to the present disclosure is able to simply analyze large amounts of samples as compared to the existing method for making a library, which is effectively usable for analysis of target DNA sequences or for database construction, in large amounts of samples.

Abstract: A method of sequencing a polynucleotide strand can include providing the polynucleotide strand with a primer annealed thereto and a polymerase operably bound to the polynucleotide strand; successively exposing the polynucleotide strand to the flow of four different dNTPs according to a first predetermined ordering; and successively exposing the polynucleotide strand to the flow of four different dNTPs according to a second predetermined ordering, wherein the second predetermined ordering is different from the first predetermined ordering.

Abstract: A method of detecting death of a cell type or tissue in a subject is disclosed. The method comprises determining whether cell-free DNA comprised in a fluid sample of the subject is derived from the cell type or tissue, wherein the determining is effected by ascertaining the methylation status of at least four methylation sites on a continuous sequence of the cell-free DNA, the sequence comprising no more than 300 nucleotides, wherein a methylation status of each of the at least four methylation sites on the continuous sequence of the DNA characteristic of the cell type or tissue is indicative of death of the cell type or tissue. Kits for detecting cell death are also disclosed.

Abstract: Provided is a method of facilitating the determination of treatment of a subject displaying atrial fibrillation, the method comprising determining a level of pitx2c expression in a sample from the subject and selecting a treatment based upon the level of pitx2c expression. Treatment with a sodium channel blocker may be selected if the level of pitx2c expression is determined to be reduced or below a predetermined threshold. Also provided are an assay system and a kit for use in the methods of the invention.

Abstract: The present invention provides assay systems and methods for detection of copy number variation at one or more loci and polymorphism detection at one or more loci in a mixed sample from an individual.

Abstract: A method that includes measuring the expression level of at least one transposon in a biological sample from a subject; and determining whether the measured transposon expression exceeds a predetermined level, and if so, administering to the subject a transposon inhibitor in an amount effective to reduce the expression level of a transposon.

Abstract: The present invention encompasses type-I IFN and IFN?-induced PD marker expression profiles, kits, and methods for identifying such IFN?-induced PD marker expression profiles. The type-I IFN and IFN?-induced PD marker expression profiles may also be used in, for example, methods of treating patients having a type-I IFN or IFN?-mediated disorder, methods of monitoring disease progression of patients receiving treatment with a therapeutic agent that binds to and modulates IFN? activity, identifying patients as candidates to receive a therapeutic that binds to and neutralizes IFN? activity, and in diagnosing or providing a prognosis to patients having IFN?-induced disorders.

Abstract: The invention features methods to predict the response to a cardiac therapy in a patient suffering from a cardiac disease, e.g., heart failure. The invention features measurement expression of biomarkers that help in this prediction. The invention also features methods for treatment of cardiac diseases. These methods include cardiac resynchronization therapy and miRNA based therapeutics.

Abstract: Described herein are methods, assays, and systems related to the prognosis, diagnosis, and treatment of Hermansky Pudlak Syndrome (HPS) and, e.g., pulmonary fibrosis in a subject. As described herein, subjects with HPS who have, will develop, Cor are most at risk or developing pulmonary fibrosis have elevated levels of CHI3L1. In some embodiments, the methods comprise administering an agonist of IL-13R?2 or an inhibitor of CRTH2 to the subject.

Abstract: Methods, systems (e.g., computer systems), compositions, and kits are provided for predicting whether an individual will respond to treatment with a TNF inhibitor, for determining a treatment regimen for an individual (e.g. a therapy that does or does not include administration of a TNF inhibitor), and for treating an individual. The subject methods include measuring an expression level of an RGS1 expression product and/or an expression level of an IL11 expression product in a biological sample from an individual. In some cases, the methods include a step of calculating a TNF inhibitor signature score from measured expression levels (e.g., calculating a geometric mean of the expression levels of an RGS1 expression product and an IL11 expression product). After comparing measured expression levels and/or a calculated TNF inhibitor signature score with a reference, one can predict whether an individual will respond to treatment with a TNF inhibitor.

Abstract: Methods and tools are provided for detecting and predicting lung cancer. The methods and tools are based on epigenetic modification due to methylation of genes in lung cancer or pre-lung cancer. The tools can be assembled into kits or can be used separately. Genes found to be epigenetically silenced in association with lung cancer include ACSL6, ALS2CL, APC2, ARTS-1, BEX1, BMP7, BNIP3, CBR3, CD248, CD44, CHD5, DLK1, DPYSL4, DSC2, EDNRB, EPB41L3, EPHB6, ERBB3, FBLN2, FBN2, FOXL2, GNAS, GSTP1, HS3ST2, HPN, IGFBP7, IRF7, JAM3, LOX, LY6D, LY6K, MACF1, MCAM, NCBP1, NEFH, NID2, PCDHB15, PCDHGA12, PFKP, PGRMC1, PHACTR3, PHKA2, POMC, PRKCA, PSEN1, RASSF1A, RASSF2, RBP1, RRAD, SFRP1, SGK, SOD3, SOX17, SULF2, TIMP3, TJP2, TRPV2, UCHL1, WDR69, ZFP42, ZNF442, and ZNF655.

Abstract: The present invention concerns differential therapeutic treatment of cancer patients based on prognostic antigen/antibody profiles used for predicting (prognosticating) a clinical response (efficacy) and/or adverse event to an immunotherapy for treatment of a malignancy in a subject, and for treating or delaying the onset or relapse of a malignancy in a subject.

Abstract: The present invention relates to a method for the diagnosis or the prognosis of metastasis, relapse or recurrence in prostate cancer which comprises determining if the c-MAF gene is amplified in a primary tumor sample. Likewise, the invention also relates to a method for the diagnosis or the prognosis of metastasis, relapse or recurrence in prostate cancer, as well as to a method for determining the tendency to develop bone metastasis with respect to metastasis in other organs, which comprise determining the c-MAF expression level. Finally, the invention relates to the use of a c-MAF inhibitor as therapeutic target for treating the prostate cancer.

Abstract: Methods for treating breast and bladder cancer patients based upon E2F4 regulatory activity as a predictor of relapse of a patient with estrogen receptor positive breast cancer and in bladder cancer stratification are provided. The methods involve determining gene expression profiles for substances linked to activity of E2F4 in cancer cells and tissue. Signature gene expression profiles are provided for identifying breast cancer samples and bladder cancer samples that would be responsive to certain therapies and also provide for prognosis based on these profiles.

Abstract: An object of the present invention is to provide a kit or a device for the detection of prostate cancer and a method for detecting prostate cancer. The present invention provides a kit or a device for the detection of prostate cancer, comprising a nucleic acid capable of specifically binding to a miRNA in a sample of a subject, and a method for detecting prostate cancer, comprising measuring the miRNA in vitro.

Abstract: The present disclosure provides gene and gene sets, the expression of which is important in the classification and/or prognosis of cancer, in particular of renal cell carcinoma.

Abstract: An apparatus to move and preheat metal material (M) to be fed to a container comprises a containing structure, having an internal compartment and provided with a support wall, a conveyor for the material (M), a fume transit section whose volume reduces as it is distanced from said container along the longitudinal development of said containing structure, and a collector for hot fumes (F) whose volume increases in a manner correlated to said reduction in the fume transit section. The collector is located below said conveyor inside the internal compartment essentially along the entire longitudinal development of said containing structure. Moreover, one or more through apertures are made in said support wall to put the conveyor and the collector into fluidic connection.

Abstract: Steel compositions are provided. A steel composition may include iron; from 0.015 to 0.06 wt. % carbon; from 9 to 12 wt. % chromium; from 0.75 to 1.5 wt. % manganese; from 0.08 to 0.18 wt. % molybdenum; from 0.10 to 0.30 wt. % silicon; from 0.2 to 1.0 wt. % vanadium; from 0.05 to 1.2 wt. % niobium; and optionally, an amount of an additional precipitate forming alloying element. Methods of making the steel compositions are also provided.

Abstract: Provided is an Alnico alloy that is lean (i.e., lower) in Co content and yet at least retains substantially the same or better magnetic properties as the corresponding more costly, commercially available Alnico grade alloy with higher Co content, such as grades 8 and 9, which include 31.5 atomic % or more Co, in order to save significant material costs.

Abstract: A method of hardening a clothing wire for processing textile fibres and to an apparatus system therefor. The clothing wire has a succession of teeth arranged in its longitudinal direction, and the clothing wire is guided through a heating region in a pass-through direction for contact with at least one open flame. The heating region is followed by a quenching bath having a quenching liquid and by a subsequent tempering apparatus. The clothing wire moving in the pass-through direction is flushed around with a protective medium in a transition region between the region of contact with the open flame and the entry into the quenching liquid.

Abstract: A primary recrystallization annealed sheet obtainable after an intermediate step of nitriding treatment in a grain-oriented electrical steel sheet production process using, as a material, a steel slab contains, in mass %, 0.001% to 0.10% of C, 1.0% to 5.0% of Si, 0.01% to 0.5% of Mn, 0.002% to 0.040% of one or two selected from S and Se, 0.001% to 0.050% of sol.Al, and 0.0010% to 0.020% of N, the remainder being Fe and incidental impurities. A nitrogen increase ?N due to the nitriding treatment is 1000 ppm or less, and N intensity according to X-ray fluorescence at the steel sheet surface is 0.59 or greater. As a result, uniform sheet thickness direction dispersion of nitrides as inhibitors can be achieved with industrial reliability in a grain-oriented electrical steel sheet production process including nitriding, thereby enabling reliable production of grain-oriented electrical steel sheets having good magnetic properties.

Abstract: A high-carbon hot-rolled steel sheet having a chemical composition containing, by mass %, C: more than 0.40% and 0.63% or less, Si: 0.10% or less, Mn: 0.50% or less, P: 0.03% or less, S: 0.010% or less, sol.Al: 0.10% or less, N: 0.0050% or less, B: 0.0005% or more and 0.0050% or less, and at least one of Sb, Sn, Bi, Ge, Te, and Se in an amount of 0.002% or more and 0.030% or less in total. The steel sheet has a microstructure including ferrite and cementite, in which the density of cementite in ferrite grains is 0.13 pieces/?m2 or less. Additionally, the steel sheet has a hardness of 81 or less in terms of HRB and a total elongation of 33% or more.

Abstract: A high-carbon hot-rolled steel sheet having a chemical composition containing, by mass %, C: 0.20% or more and 0.40% or less, Si: 0.10% or less, Mn: 0.50% or less, P: 0.03% or less, S: 0.010% or less, sol.A1: 0.10% or less, N: 0.0050% or less, B: 0.0005% or more and 0.0050% or less, and at least one of Sb, Sn, Bi, Ge, Te, and Se in an amount of 0.002% or more and 0.030% or less in total. The steel sheet has a microstructure including ferrite and cementite, in which the density of cementite in the ferrite grains is 0.08 pieces/m2 or less. Additionally, the steel sheet has a hardness of 73 or less in terms of HRB and a total elongation of 39% or more.

Abstract: A hot-rolled steel sheet having a composition comprising, in mass %, C: 0.06 to 0.3%, Si: 0.4 to 2.5%, Mn: 0.6 to 3.5%, P: at most 0.1%, S: at most 0.05%, Ti: 0 to 0.08%, Nb: 0 to 0.04%, a total content of Ti and Nb: 0 to 0.10%, sol.Al: 0 to 2.0%, Cr: 0 to 1%, Mo: 0 to 0.3%, V: 0 to 0.3%, B: 0 to 0.005%, Ca: 0 to 0.003%, REM: 0 to 0.003%, the remainder Fe and impurities, is cold rolled, then heated at an average heating rate of at least 15° C./sec so that a proportion of an unrecrystallization of a region not transformed to austenite at a time of reaching (Ac1 point+10° C.) is at least 30 area %, and then held for 30 seconds at temperature of at least (0.9×Ac1 point+0.1×Ac3 point) and at most (Ac3 point+100° C.).

Abstract: A method for manufacturing a plate, a band, or a coil of hot-rolled steel is provided. The method includes providing an ingot or a slab of steel with a desired composition and a microstructure composed of austenite and 35 to 65% ferrite by volume and hot rolling the ingot or slab at a temperature between 1150 and 1280° C. to obtain a plate, a band or a coil. A method for manufacturing a hot-rolled bar or wire of steel, a steel profile and a forged steel piece are also provided.

Abstract: A method of extracting germanium from a germanium deposit using a thermal reduction process is disclosed. The present disclosure relates to a method of extracting germanium, which belongs to the field of metallurgy technologies of nonferrous metal, and particularly relates to a method of extracting germanium from a germanium deposit through thermal reduction, volatilization and concentration using sodium monophosphate as a reducing agent. The method of the present disclosure includes: adding sodium monophosphate to a germanium deposit; isolating from air; increasing the temperature and baking the germanium deposit; and obtaining a germanium concentrate after volatilization and concentration of the germanium deposit. Through baking the germanium deposit at 1,000° C., volatilization and concentration of the germanium deposit, the germanium recycling rate exceeds 96% when sodium monophosphate weighing 2.5% of the germanium depositis added.

Abstract: An object of the present invention is to provide a brass alloy, in which the content of Bi is reduced to secure a good recyclability while maintaining the dezincification corrosion resistance required for a member for water works, and which is capable of exhibiting an erosion-corrosion resistance and excellent mechanical properties to be used as a member for water works. This brass alloy contains: 24% by mass or more and 34% by mass or less of Zn; 0.5% by mass or more and 1.7% by mass or less of Sn; 0.4% by mass or more and 1.8% by mass or less of Al; 0.005% by mass or more and 0.2% by mass or less of P; and 0.01% by mass or more and 0.25% by mass or less of Pb; with the balance being copper and an unavoidable impurity(ies).

Abstract: Processes for producing low nitrogen, essentially nitride-free chromium or chromium plus niobium-containing nickel-based alloys include charging elements or compounds which do not dissolve appreciable amounts of nitrogen in the molten state to a refractory crucible within a vacuum induction furnace, melting said elements or compounds therein under reduced pressure, and effecting heterogeneous carbon-based bubble nucleation in a controlled manner. The processes also include, upon cessation of bubble formation, adding low nitrogen chromium or a low nitrogen chromium-containing master alloy with a nitrogen content of below 10 ppm to the melt, melting and distributing said added chromium or chromium-containing master alloy throughout the melt, bringing the resulting combined melt to a temperature and surrounding pressure to permit tapping, and tapping the resulting melt, directly or indirectly, to a metallic mold and allowing the melt to solidify and cool under reduced pressure.

Abstract: Provided are an aluminum alloy for die casting, suitable for important safety-related components in automobiles without significantly worsening corrosion resistance even though containing Cu at a ratio capable of providing an effect of improving mechanical characteristics, and an aluminum alloy die cast obtained through die-casting the alloy. More specifically, the present invention is directed to an aluminum alloy for die casting containing, in wt %, 0.03%<Cu?0.7%, 6.0%<Si?11.0%, 0.15%?Mg?0.50%, 0.05%?Fe?0.6%, 0.05%?Ti?0.25%, Mn?0.8%, 0.10%?Cr?0.40%, and for the remaining portion, Al and unavoidable impurities.

Abstract: This relates to an aluminium alloy, a method for producing a lightweight metal workpiece, a lightweight metal workpiece comprising the aluminium alloy, and also the use of the aluminium alloy for producing heat-resistant lightweight metal workpieces by means of additive layer manufacturing (ALM) and/or spraying methods or for engine components, cylinder heads, crank housings, heat-resistant safety component parts, air-conditioning system components, structural aircraft component parts, in particular in the case of supersonic aircraft, power unit segments, pylons, or as coating material for component parts.

Abstract: New wrought 7xxx aluminum alloys are disclosed. The new wrought 7xxx aluminum alloys generally include from 3.75 to 8.0 wt. % Zn, from 1.25 to 3.0 wt. % Mg, where the wt. % Zn exceeds the wt. % Mg, from 0.35 to 1.35 wt. % Cu, from 0.04 to 0.20 wt. % V, from 0.06 to 0.20 wt. % Zr, where V+Zr?0.23 wt. %, from 0.01 to 0.25 wt. % Ti, up to 0.50 wt. % Mn, up to 0.40 wt. % Cr, up to 0.35 wt. % Fe, and up to 0.25 wt. % Si, the balance being aluminum and impurities, wherein the wrought 7xxx aluminum alloy include not greater than 0.10 wt. % each of any one impurity, and wherein the wrought 7xxx aluminum alloy includes not greater than 0.35 wt. % in total of the impurities.

Abstract: An austenitic alloy comprising (in weight %): C: 0.01-0.05 Si: 0.05-0.80 Mn: 1.5-2 Cr: 26-34.5 Ni: 30-35 Mo: 3-4 Cu: 0.5-1.5 N: 0.05-0.15 V: ?0.15 the balance being Fe and unavoidable impurities, characterized in that 40?% Ni+100*% N?5 50.

Abstract: The invention relates to an austenitic high-manganese stainless steel having high strength and ductility. The stainless steel which has in weight % 0.03-0.1% carbon, 0.08-1.0% silicon, 14-26% manganese, 10.5-18% chromium, less than 0.8% nickel, 0.05-0.60% copper, 0.1-0.8% nitrogen and 0.0008-0.0050% boron, with the rest being iron and inevitable impurities occurred in stainless steels, and the stainless steel is cold deformable utilizing the TWIP (TWinning Induced Plasticity) mechanism.

Abstract: The present disclosure is directed at alloys and method for layer-by-layer deposition of metallic alloys on a substrate to produce a metallic part. Applications for the metallic parts include pumps, pump parts, valves, molds, bearings, cutting tools, filters or screens.

Abstract: Systems and methods in accordance with embodiments of the invention implement copper-based materials in applications where resistance to wear is desired. In one embodiment, a wear-resistant gear includes a gear defined by a rotatable body having teeth disposed on an outer surface of the rotatable body, where the gear body is formed at least in part from a material including copper and X, where X is one of zirconium, titanium, hafnium, rutherfordium, and mixtures thereof and where the atomic ratio of copper to X is approximately between 2:3 and 3:2.

Abstract: Disclosed herein is a composite comprising a metal alloy matrix; where the metal alloy matrix comprises aluminum in an amount greater than 50 atomic percent; a first metal and a second metal; where the first metal is different from the second metal; and where the metal alloy matrix comprises a low temperature melting phase and a high temperature melting phase; where the low temperature melting phase melts at a temperature that is lower than the high temperature melting phase; and a contracting constituent; where the contracting constituent exerts a compressive force on the metal alloy matrix at a temperature between a melting point of the low temperature melting phase and a melting point of the high temperature melting phase or below the melting points of the high and low temperature melting phases.

Abstract: Provided is a 6000-series aluminum alloy sheet suitable for a vehicle structural component. The Mg content and the Si content of an Al—Mg—Si aluminum alloy sheet are balanced in a special relationship, particularly cube orientation is increased as a texture in a surface region of the sheet, and the yield ratio of the sheet is reduced, thereby high strength of 0.2% proof stress of 220 MPa or more required for the vehicle structural component is ensured, and crashworthiness evaluated by a VDA bending test is improved.

Abstract: Described herein are novel 7xxx series aluminum alloys. The alloys exhibit high strength. The alloys can be used in a variety of applications, including automotive, transportation, electronics, aerospace, and industrial applications. Also described herein are methods of making and processing the alloys. Further described herein are methods of producing a metal sheet, which include casting an aluminum alloy as described herein to form an ingot, homogenizing the ingot, hot rolling the ingot to produce a hot band, and cold rolling the hot band to a metal sheet of final gauge.