Abstract: A process for increasing alcohol yield from biomass (the form or agro- or forest residue, grains, hops, etc.), involving multiple hydrodynamic cavitation treatments of biomass filtrate—both before and after fermentation. Carbohydrates extracted from biomass are subjected to a first cavitation treatment to promote additional conversion into carbohydrates. The carbohydrates are then combined with bacterial species and nutrients, and allowed to ferment. The fermentation product is subjected to a second hydrodynamic cavitation treatment to promote further conversion of carbohydrates into bioalcohol. After distillation, the bioalcohol is subjected to a second hydrodynamic cavitation treatment to increase its purity.

Abstract: The present invention relates to methods for production of 2?Fucosyllactose by a microbial system comprising a ?-1,2 fucosyltransferase (FucT2) polynucleotide and a Guanosine 5?-diphospho-?-L-fucose (GDP-L-fucose) synthesis pathway using lactose as a substrate. Furthermore, the present invention relates to compositions comprising the microbial system.

Abstract: The invention relates to a method for producing a lysate used for cell-fee protein biosynthesis, comprising the following steps: a) a genomic sequence in an organism, which codes for an essential translation product that reduces the yield of cell-fee protein biosynthesis, is replaced by the foreign DNA located under a suitable regulatory element, said foreign DNA coding for the essential translation product that additionally contains a marker sequence; b) the organism cloned according to step a) is cultivated; c) the organisms from the culture obtained in step b) are lysed; and d) the essential translation product is eliminated by means of a separation process that is selective for the marker sequence. Also discussed are said lysate and the use thereof.

Abstract: The present disclosure relates to methods of producing antibodies with increased levels of non-fucosylated glycoforms by culturing mammalian cells in culture media with enhanced concentrations of glycine relative to traditional basal media.

Abstract: A method for preparing a methionine-containing peptide or protein for analysis by mass spectrometry includes incubating the methionine-containing peptide or protein with a heterologous methionine sulfoxide reductase A (MsrA), MsrB, or MsrAB enzyme resulting in the reduction of oxidized methionine-containing peptides or proteins.

Abstract: The present invention relates to a process for preparing a yeast hydrolysate comprising less than 100 ppm gluten and at least 1 ppm gluten based on salt-free yeast dry matter.

Abstract: The present disclosure provides a method of identifying a substrate for a protease including contacting a protease to one of a first array and a second array, each array having the same plurality of features, each feature including at least one sequence linked to a solid support. The at least one sequence includes a candidate protease substrate linked to a reporter. The method further includes contacting a detectable element to each of the arrays to allow binding of the detectable element to the reporter, and detecting first and second signals resulting from binding of the detectable element to the each of the reporters in the first and second arrays. The method further includes comparing the first signal and the second signal to identify a difference in the first signal and the second signal, and identifying at least one candidate protease substrate as a substrate for the protease.

Abstract: Provided is a process for producing a substrate solution for measuring lipase activity including, as a substrate for measuring lipase activity, 1,2-o-dilauryl-rac-glycero-3-glutaric acid (6?-methylresorufin) ester, wherein the production process does not necessitate cumbersome or special processing such that skill is required, or does not necessitate a special apparatus, instruments, or other items. This production process is a process for producing a substrate solution for measuring lipase activity, and is characterized by including the steps of (1) mixing the substrate for measuring lipase activity and a side-chain-type nonreactive polyether-modified-type modified silicone oil or a polyoxyethylene/polyoxypropylene condensate to prepare a mixture, and (2) mixing all or a portion of the mixture of step (1) with water or an aqueous solution.

Abstract: The present invention relates to compositions and methods for preserving and extracting nucleic acids from saliva. The compositions include a chelating agent, a denaturing agent, buffers to maintain the pH of the composition within ranges desirable for DNA and/or RNA. The compositions may also include a reducing agent and/or antimicrobial agent. The invention extends to methods of using the compositions of the invention to preserve and isolate nucleic acids from saliva as well as to containers for the compositions of the invention.

Abstract: The present invention relates to compositions, methods and kits which can be used to amplify nucleic acids with the advantage of decreasing user time and possible contamination. The dried reagent composition of the invention can be used for easy processing and amplification of nucleic acid samples.

Abstract: Provided herein are methods and kits directed to a method of assaying a sample to determine the presence of a source of pink discoloration defect of a dairy product, such as cheese. In some embodiments, the method comprises the steps of assaying the sample to detect the presence of a Thermus species of bacteria, such as Thermus thermophilus bacteria, wherein detection of Thermus thermophilus bacteria in the sample indicates that the sample contains a source of pink discoloration of a dairy product.

Abstract: The present invention, SMASH (Short Multiply Aggregated Sequence Homologies), is a technique designed to pack multiple independent mappings into every read. Specifically, the invention relates to a composition comprising a first mixture of different chimeric genomic nucleic acid fragments, wherein each different fragment in the mixture comprises randomly ligated DNA segments, wherein each DNA segment in the fragment is a nucleic acid molecule at least 27 base pairs in length resulting from random fragmentation of a single genome. The invention also relates to methods for generating said composition and use of said composition to obtain genomic information, for example, copy number variation.

Abstract: A methodology for assays and diagnostics utilizes a nanoporous or corrugated metal-containing surface, fiber or particle which enhances or suppresses the optical detectability of a label. The resulting optical, electromagnetic, or imaging signal signals the presence of a pathogen or analyte of interest. Preferred embodiments pertain to label-free, in situ monitoring of individual DNA hybridization in microfluidics using molecular sentinel probes immobilized on nanoporous gold disks. By immobilizing molecular sentinel probes on nanoporous gold disks, single-molecule sensitivity is demonstrated via surface-enhanced Raman scattering which provides robust The described methodology is generally applicable to most amplification independent assays and molecular diagnostics.

Abstract: Method of detecting a signal from droplets for instrument calibration. In the method, a calibration standard for a droplet detection instrument may be received. The calibration standard may have been shipped at least one kilometer when received. The calibration standard may include a mixture of first droplets and second droplets. Each of the first and second droplets may be encapsulated by an immiscible carrier liquid and may have a stabilizing droplet skin that is formed by heating and that includes a skin-forming protein. Each the first droplets may contain a fluorescent dye, and each of the second droplets may contain a fluorescent dye. A fluorescence signal may be detected from a plurality of the first and second droplets with a droplet detection instrument. The fluorescence signal may be stronger for the first droplets than the second droplets.

Abstract: A new kit is provided for detecting or quantifying the amount of one or multiple nucleic acid targets in a biological sample, and a detection or quantification method using this kit. said the present kit is constituted by a universal energy donor probe, a universal energy acceptor probe and a couple of adaptors.

Abstract: Disclosed is a cell selection method including a sample preparation step of preparing a sample by performing staining of nucleic acid in each of cells by a first fluorescent dye; and hybridization with respect to an evaluation target region in DNA in each cell by an evaluation probe including a second fluorescent dye; a light receiving step of applying light to the sample and receiving fluorescence from the first fluorescent dye and fluorescence from the second fluorescent dye; and a selection step of selecting an analysis target cell on the basis of intensity of the fluorescence from the first fluorescent dye and intensity of the fluorescence from the second fluorescent dye, wherein the first fluorescent dye is a dye that emits fluorescence having a first wavelength, and the second fluorescent dye is a dye that emits fluorescence having a second wavelength different from the first wavelength.

Abstract: The present invention relates to assays, including amplification assays, conducted in the presence of modulators. These assays can be used to detect the presence of particular nucleic acid sequences. In particular, these assays can allow for genotyping or other genetic analysis.

Abstract: A high density DNA array comprising a patterned surface, said surface comprising a pattern of small DNA binding regions separated by a non-DNA binding surface, wherein the DNA binding regions comprise DNA capture chemistry and the non-DNA binding surface does not have the DNA capture chemistry wherein more than 50% of the DNA binding regions in the array have single informative DNA species.

Abstract: A method for detecting the presence or absence of heteroploidy of fetal chromosomes includes: obtaining a primary amplification products by amplifying chromosomal DNA obtained from a biological sample collected from a pregnant mother; performing multiplex amplification of a plurality of target regions to obtain a secondary amplification product using the primary amplification product as a template; adding a label to both terminals of the secondary amplification product to obtain the labeled secondary amplification product; performing amplification using a primer pair annealing to the label using the labeled secondary amplification product as a template to obtain a tertiary amplification product; and determining the amplification amount and base sequences of the plurality of target regions from the tertiary amplification product. The primary amplification amplifies the total amount of DNA by 6000 to 30000 times, and the secondary amplification step amplifies the total amount of DNA by 3 to 150 times.

Abstract: Provided are novel methods useful for the screening and generation of potential cosmetic agents that work in synchronization with the circadian rhythm of the skin for the treatment of aged skin. These novel methods allow for identification of new cosmetic agents that can be screened for their selective treatment of skin aging conditions and for the specific targeting of particular skin cell types, such as keratinocytes or fibroblasts.

Abstract: The present invention relates generally to the fields of reproductive medicine. More specifically, the present invention relates to in vitro non-invasive methods for determining the quality of an embryo by determining the level of the cell free nucleic acids or miR-29a or let7-b in the nucleic acid extract from a follicular fluid sample.

Abstract: Described herein are the association BV677278 (READ1) with reading disability and language impairment, as well as the synergistic interaction of DCDC2 risk haplotypes or alleles with KIAA0319 risk allele.

Abstract: The present invention provides methods and tests that allow for the establishment of personalized weight-management programs for an individual based upon the individual's genotype in the glutathione S-transferase pi gene and/or the interleukin-6 gene. Methods are disclosed for determining the individual's genotype, which may be used to select an appropriate therapeutic/dietary program or lifestyle recommendation. Such a personalized weight-management program will have obvious benefits (e.g., yield better results in terms of weight loss and weight maintenance) over traditional weight-management programs that do not take into account genetic information.

Abstract: Disclosed herein is a method for diagnosing a renal allograft recipient's risk for developing fibrosis of the allograft and allograft loss. The method includes determining the expression levels of certain microRNAs, which have been determined to be predictive of an allograft recipient's risk. Also disclosed herein is a method of treating a renal allograft recipient to inhibit fibrosis of the allograft and allograft loss, as well as kits for use in the methods disclosed herein.

Abstract: The present invention relates generally to the fields of reproductive medicine. More specifically, the present invention relates to methods for determining the ovarian reserve and ovarian function by determining the level of cell-free nucleic acids or the level of a miRNA species selected from miR_30_a or miR_140 or let_7_b or miR_191 or miR_320_a or miR_21 or miR_30_d or miR_574_3p. The method may also be used in predicting the efficacy of controlled ovarian hyperstimulation.

Abstract: The Invention relates to an ex vivo method of diagnosing or predicting a hereditary spastic paraplegias (HSP), in a subject, which method comprises detecting a mutation in the ZFYVE26 gene or protein (spastizin), wherein said mutation is indicative of a hereditary spastic paraplegias (HSP).

Abstract: The invention described herein relates to methods of treating emphysema and COPD with a GHK tripeptide. The invention further relates to methods of determining the state of the lungs using biomarkers described herein.

Abstract: The invention is a novel MECP2E1 splice variant and its corresponding polypeptide. The invention also includes methods of using these nucleic acid sequences and proteins in medical diagnosis and treatment of neuropsychiatric disorders or development disorders.

Abstract: The invention provides a method of diagnosing a disease or a predisposition to contract a disease by assaying for mutations of uromodulin (UMOD) within a test subject or patient. The presence of a mutation in the UMOD supports a diagnosis of a disease or a predisposition to contract a disease within the patient.

Abstract: The present disclosure describes the use of genetic variance information for folate transport or metabolism genes or pyrimidine transport or metabolism genes in the selection of effective methods of treatment of a disease or condition. The variance information is indicative of the expected response of a patient to a method of treatment. Methods of determining relevant variance information and additional methods of using such variance information are also described.

Abstract: The present invention provides novel mitochondrial fusion transcripts and the parent mutated mtDNA molecules that are useful for predicting, diagnosing and/or monitoring cancer. Hybridization probes complementary thereto for use in the methods of the invention are also provided.

Abstract: The present invention provides a method of identifying a subject for whom a prostate biopsy is indicated, comprising: a) determining, from a nucleic acid sample obtained from the subject, a genotype for the subject at a plurality of biallelic polymorphic loci, wherein each of said plurality has an associated allele and an unassociated allele, wherein the genotype is selected from the group consisting of homozygous for the associated allele, heterozygous, and homozygous for the unassociated allele; b) calculating a genetic risk score (GRS) for the subject based on the genotype determined in step (a); and c) analyzing the GRS of the subject in combination with a prostate specific antigen (PSA) level of the subject to identify a prostate cancer detection rate for the subject, whereby a prostate cancer detection rate of greater than or equal to a reference value identifies the subject as a subject for whom a prostate biopsy is indicated.

Abstract: Provided are methods useful for determining the expected efficacy of certain chemotherapy treatments and methods of treating cancer based on said determination. In particular, provides are methods and procedures of predicting the efficacy of paclitaxel treatments in cancer patients.

Abstract: The present invention relates to the discovery of a method for identifying a treatment regimen for a patient diagnosed with cancer, predicting patient resistance to therapeutic agents and identifying new therapeutic agents. Specifically, the present invention relates to the use of an algorithm to identify a mutation in a kinase, determine if the mutation is an activation or resistance mutation and then to suggest an appropriate therapeutic regimen. The invention also relates to the use of a pattern matching algorithm and a crystal structure library to predict the functionality of a gene mutation, predict the specificity of small molecule kinase inhibitors and for the identification of new therapeutic agents.

Abstract: Biomarkers and methods using the biomarkers for classifying cancer in a patient (e.g., predicting the risk of cancer-specific death or cancer recurrence) are provided.

Abstract: Described herein are methods, compositions and kits directed to the detection of gene dysregulations such as those arising from gene fusions and/or chromosomal abnormalities, e.g., translocations, insertions, inversions and deletions. Samples containing dysregulated gene(s) of interest may show independent expression patterns for the 5? and 3? regions of the gene. The methods, compositions and kits are useful for detecting mutations that cause the differential expression of a 5? portion of a target gene relative to the 3? region of the target gene.

Abstract: Single-stranded oligonucleotide probes, systems, kits and methods for chromosome enumeration, gene copy enumeration, or tissue diagnostics. The probes are particularly suited for detecting gene amplification, deletion, or rearrangement in tissue samples in a single, dual, or multiplexed assay. The probes exhibit improved performance compared to industry leading dual-stranded probes; particularly in terms of the rate of hybridization and the ability to achieve specific hybridization without blocking DNA.

Abstract: The present invention provides an artificial exogenous reference molecule for type and abundance comparison among different species of microorganisms. The artificial exogenous reference molecule includes several species detection regions and several linker sequence regions, where the species detection regions and the linker sequence regions are arranged alternately; at least two species detection regions are provided and are species detection regions for different species; and the linker sequence regions are random sequences different from species sequences to be detected. The species detection region includes a primer sequence region and a specific recognition region; the sequence length of the specific recognition region simulates the characteristic detection sequence length of the corresponding species to be detected; and the sequence of the specific recognition region has obviously specific difference from the DNA sequence of a genome in the species to be detected without homology.

Abstract: Markers associated with Sclerotinia whole plant field resistance are provided. Methods of identifying Sclerotinia resistant and susceptible plants, using the markers are provided. Methods for identifying and isolating QTLs are a feature of the invention, as are QTLs associated with Sclerotinia whole plant field resistance.

Abstract: Systems and methods for detecting and/or identifying target cells (e.g., bacteria) using engineered transduction particles are described herein. In some embodiments, a method includes mixing a quantity of transduction particles within a sample. The transduction particles are associated with a target cell. The transduction particles are non-replicative, and are engineered to include a nucleic acid molecule formulated to cause the target cell to produce a series of reporter molecules. The sample and the transduction particles are maintained to express the series of the reporter molecules when target cell is present in the sample. A signal associated with a quantity of the reporter molecules is received. In some embodiments, a magnitude of the signal is independent from a quantity of the transduction particle above a predetermined quantity.

Abstract: Provided herein are a GeXP rapid detection primer set for simultaneously identifying gene HA of eight different human-infected subtypes of avian influenza virus, a kit, and use thereof. Disclosed are 9 pairs of specific primer and 1 pair of universal primer, for a GeXP rapid detection primer kit for simultaneously identifying gene HA of eight different human-infected subtypes of avian influenza virus. Eight different human-infected subtypes HA of avian influenza virus from the nine genes of M, H1, H2, H3, H5, H6, H7, H9 and H10 can be identified simultaneously with a sensitivity of 102 copies/?L.

Abstract: A rolled steel bar for machine structural use includes a predetermined chemical composition. In the rolled steel bar for machine structural use, K1 obtained from “K1=C+Si/7+Mn/5+1.54×V” is 0.95 to 1.05, K2 obtained from “K2=139?28.6×Si+105×Mn?833×S?13420×N” is more than 35, a Mn content and a S content satisfy Mn/S?8.0, and a total decarburized depth of a surface layer is 500 ?m or less.

Abstract: A method for producing a high strength coated steel sheet having a yield stress YS>800 MPa, a tensile strength TS>1180 MPa, and improved formability and ductility. The steel contains: 15%?C?0.25%, 1.2%?Si?1.8%, 2%?Mn?2.4%, 0.1% 23 Cr?0.25%, Al?0.5%, the remainder being Fe and unavoidable impurities. The sheet is annealed at a temperature higher than Ac3 and lower than 1000° C. for a time of more than 30 s, then quenched by cooling it to a quenching temperature QT between 250° C. and 350° C., to obtain a structure consisting of at least 60% of martensite and a sufficient austenite content such that the final structure contains 3% to 15% of residual austenite and 85% to 97% of martensite and bainite without ferrite, then heated to a partitioning temperature PT between 430° C. and 480° C. and maintained at this temperature for a partitioning time Pt between 10 s and 90 s, then hot dip coated and cooled to the room temperature.

Abstract: A method for manufacturing a high-strength galvanized steel sheet includes performing hot rolling, cold rolling, first annealing, pickling, and second annealing. The first annealing is performed to obtain a steel sheet having a steel microstructure including ferrite in an amount of 10% or more and 60% or less in terms of area ratio, and martensite, bainite, and retained austenite in a total amount of 40% or more and 90% or less in terms of area ratio. The second annealing includes heating to an annealing temperature of 750° C. or higher and 850° C. or lower, holding at the annealing temperature for 10 seconds or more and 500 seconds or less, cooling at an average cooling rate of 1° C./s or more and 15° C./s or less, performing a galvanizing treatment, and cooling to a temperature of 150° C. or lower at an average cooling rate of 5° C./s or more and 100° C./s or less.

Abstract: A multipurpose continuous processing line able for heat treating and hot dip coating a steel strip comprising: —an annealing section (1) for heating the steel strip to a predetermined annealing temperature and for maintaining the steel strip at said annealing temperature, —a first transfer section (2), —an overaging section (3) able to maintain the temperature of the steel strip between 300° C. and 700° C., —a second transfer section (4) able to adjust the temperature of the steel strip to allow the hot dip coating of the strip and, —a hot dip coating section (5), wherein the first transfer section (2) comprises, in sequence, cooling means (21) and heating means (22).

Abstract: In a method of treating a nitrided/nitrocarburized workpiece, at least a portion of the workpiece is subjected to a first step in which at least one laser beam is moved in at least one pass over the portion, until the surface layer of the portion is transformed in part or in full, and until the distribution of the nitrogen concentration in the diffusion zone is modified. In a second step at least one laser beam is moved in at least one pass over said portion so as to enable the nitrogen concentration in the underlying diffusion layer to be reduced.

Abstract: A method of recovering a metal from a low-grade ore which is subjected to cyanide leaching to produce a PLS which contains a metal cyanide which is removed from the PLS by ultrafiltration and nano-filtration, and then acidified and sulphidised to produce a metal sulphide from which the metal is extracted, and hydrogen cyanide which is recycled to the cyanide leaching step.

Abstract: Provided is a method for producing a pellet capable of suppressing heat shock-induced crack occurrence, when nickel oxide ores are made into pellets and placed in a reducing furnace in a smelting process. In the method for producing a pellet from a nickel oxide ore, a nickel oxide ore, a binder and a carbonaceous reducing agent are mixed, the mixture is made into a lump, and then the resulting lump is subjected to a preheat treatment at a temperature of 350° C. to 600° C. In this preheat treatment, the lump more preferably undergoes the preheat treatment at a temperature of 400° C. to 550° C.

Abstract: Provided is a production method for producing pellets that are used for producing an iron-nickel alloy and that are produced by mixing at least a nickel oxide ore, a carbonaceous reducing agent, and an iron oxide and agglomerating the obtained mixtures, the method comprising: a step S11 for producing at least two types of mixtures having different mixing ratios of said nickel oxide ore, said carbonaceous reducing agent, and said iron oxide; and a step S12 for forming pellets, which are agglomerates having a layered structure, by using said two or more types of mixtures such that the mixture with the highest content ratio of said iron oxide, among said two or more types of mixtures that have been obtained, forms the outermost layer.

Abstract: Compositions for forming Au-based bulk-solidifying amorphous alloys are provided. The Au-based bulk-solidifying amorphous alloys of the current invention are based on ternary Au—Cu—Si alloys, and the extension of this ternary system to higher order alloys by the addition of one or more alloying elements. Additional substitute elements are also provided, which allow for the tailoring of the physical properties of the Au-base bulk-solidifying amorphous alloys of the current invention.