Abstract: The present invention features methods for selective lysis of endogenous cells in a biological sample. In preferred embodiments, the methods of the invention comprise contacting the biological sample with lysis solution, and subjecting the mixture to ultrasound, thereby selectively lysing the endogenous cells in the biological sample. The invention also features a lysis solution comprising Saponin and Proteinase.

Abstract: Provided is a device that collects vesicles and vesicle-like materials from biological fluids. Such devices comprise at least one sample loading region; at least one corresponding vesicle-capture material, wherein said vesicle-capture material comprises glass-like materials; and at least one corresponding sample receiving region, wherein passage of the biological fluid from the sample loading region through the vesicle capture material and into the sample receiving region results in the capture of vesicles. Additional methods provide for a method of isolating vesicles and vesicle-like materials from biological fluids are also provided.

Abstract: Devices, methods, and kits are disclosed for collection, labeling and analysis of samples containing a substance of interest. Such devices and methods are used in forensic, human identification, access and importation control, and other investigative technologies to collect even limited amounts of a substance of interest that may be present on a substrate and to facilitate analysis to identify the substance of interest.

Abstract: A method is described for the detection of the degree of methylation of a specific cytosine in the sequence context 5?-CpG-3? of a genomic DNA sample. In the first step, the genomic DNA is chemically treated in such a way that the cytosine bases are converted to uracil, but not the 5-methylcytosine bases. Then segments of the genomic DNA which contain the said specific cytosine are amplified, whereby the amplified products are given a detectable label and in the following steps the extent of hybridization of the amplified products on two classes of oligonucleotides is determined by detection of the label of the amplified products, and a conclusion is made on the extent of methylation of said specific cytosine in the genomic DNA sample from the ratio of the labels detected on the two classes of oligonucleotides as a consequence of the hybridization.

Abstract: A process includes providing a mixture that includes a recombinase, a single-strand binding protein, and one or more oligonucleotides; and detecting particles in the reaction mixture.

Abstract: The present invention relates to new methods and uses for the qualitative and quantitative detection of microbial nucleic acids using at least a first control nucleic acid, or a first and a second control nucleic acid in different concentrations. The method is based on amplification of nucleic acids, for example the polymerase chain reaction. Further provided are kits comprising components for performing said methods and uses.

Abstract: Disclosed herein are automated systems for performing various biochemical and molecular biological procedures, including processor-controlled execution of protocols involving multiple steps performed in, on, or with liquid microdroplets. Example protocols are the various Polymerase Chain Reaction (PCR) protocols, but the subject systems are not limited to performing PCR protocols. Formation of a microdroplet of the sample for use in the described systems is achieved by bringing an amount of the sample into contact with a hydrophobic milieu, such as a superhydrophobic surface or hydrophobic liquid.

Abstract: This invention relates to the production and genotyping of mice lacking both Angiopoietin 1 and Angiopoietin 2. This invention also relates to the use of Tie2 receptor activation for treatment of open angle glaucoma, congenital glaucoma and cystic kidney disease, and more specifically to the use of angiopoietin 1 recombinant proteins, peptides, VE-PTP phosphatase inhibitors, and Tie2— peptomimetics to improve lymphatic drainage in the Schlemm's canal and corneal limbal lymphatic system for open angle glaucoma and congenital glaucoma patients, and to slow and/or reduce the growth of cysts in patients with cystic kidney disease.

Abstract: Provided are methods of adding adapters to nucleic acids. The methods include combining in a reaction mixture a template nucleic acid, a template switch oligonucleotide, a polymerase, and dNTPs. The reaction mixture components are combined under conditions sufficient to produce a product nucleic acid that includes the template nucleic acid and the template switch oligonucleotide each hybridized to adjacent regions of a single product nucleic acid including a region polymerized from the dNTPs by the polymerase. The methods further include attaching sequencing platform adapter constructs to ends of the product nucleic acid or a derivative thereof. Aspects of the invention further include compositions and kits.

Abstract: The present invention relates to amplification primers with a universal tag and sequencing primers comprising at least one non-natural nucleobase capable of hybridizing to a complementary non-natural nucleobase. The present invention further relates to amplification methods of nucleic acid amplification and sequencing using an amplification primer with a universal tag and sequencing primers, as well as kits and solid supports comprising such primers and tags.

Abstract: This invention provides substrates for use in various applications, including single-molecule analytical reactions. Methods for propagating optical energy within a substrate are provided. Devices comprising waveguide substrates and dielectric omnidirectional reflectors are provided. Waveguide substrates with improved uniformity of optical energy intensity across one or more waveguides and enhanced waveguide illumination efficiency within an analytic detection region of the arrays are provided.

Abstract: This invention provides methods for attaching a nucleic acid to a solid surface and for sequencing nucleic acid by detecting the identity of each nucleotide analog after the nucleotide analog is incorporated into a growing strand of DNA in a polymerase reaction. The invention also provides nucleotide analogs which comprise unique labels attached to the nucleotide analog through a cleavable linker, and a cleavable chemical group to cap the —OH group at the 3?-position of the deoxyribose.

Abstract: Methods and materials for detection of aneuploidy and other chromosomal abnormalities using fetal tissue are disclosed. Results can be obtained rapidly, without cell culture. The method uses digital PCR for amplification and detection of single target sequences, allowing an accurate count of a specific chromosome or chromosomal region. Specific polynucleic acid primers and probes are disclosed for chromosomes 1, 13, 18, 21, X and Y. These polynucleic acid sequences are chosen to be essentially invariant between individuals, so the test is not dependent on sequence differences between fetus and mother.

Abstract: The invention provides methods and compositions for early diagnosis and treatment of a disease associated with a specific antibody by employing the detection of a cross-idiotypic epitope on the specific antibody to detect the cells that produce the antibody before the development of clinical symptoms of the disease.

Abstract: Methods and kits for diagnosis, prognosis and treatment of metastatic tumors are provided where the metastatic tumor is characterized by changes in expression of +++, ++ and/or 11a variants of Mena.

Abstract: Compositions and methods associated with recurrent MIPOL1-ETV1 genetic rearrangements that are useful for cancer diagnosis and therapy are disclosed.

Abstract: The present disclosure provides for characterization of normal flora and identifying biomarkers in the gut of healthy, neruotypical subjects. Aspect of the disclosure provide for the characterization of the gut microbiome in ADS subjects, characterized by reduced richness and significant loss of the ‘Prevotella-like enterotype’ compared to neurotypical subjects. The relative abundance of genera Prevotella, Coprococcus, Prevotellaceae and Veillonellaceae are significantly lower in autistic children than in neurotypical children. Further, Prevotella, is one of the three main classifiers for the human enterotypes, along with Bacteroides and Ruminococcus. These three core genera are among main contributors in the principle component analysis. ‘Prevotella-like enterotype’ was absent in the autistic group, while neurotypical samples showed an even distribution among the three enterotypes.

Abstract: The invention provides a transgenic Glycine max event MON87751, plants, plant cells, seeds, plant parts, progeny plants, and commodity products comprising event MON87751. The invention also provides polynucleotides specific for event MON87751, plants, plant cells, seeds, plant parts, and commodity products comprising polynucleotides for event MON87751. The invention also provides methods related to event MON87751.

Abstract: An expression vector, comprising a first reporter nucleic acid sequence operably linked to a first expression control sequence comprising a promoter; and a second reporter nucleic acid sequence operably linked to a second expression control sequence that comprises a response element that is activated or inactivated as one or more of the cells differentiate or dedifferentiate. Methods and kits for imaging and monitoring stem cells comprising the expression vector are also provided.

Abstract: BioMEMS/NEMS appliance biologically monitors an individual, using biosensors to detect cellular components. Data is simulated or analyzed using systems-biology software, which provides diagnostic or therapeutic guidance.

Abstract: The furnace of the present invention includes a body of a furnace having a cylindrical shape; a steel shell which is arranged at an inside surface of the furnace; and a lining refractory which is arranged at an inside of the steel shell and includes a plurality of refractory blocks, wherein: each of the refractory blocks includes a hot-face end surface which has a hexagonal shape exposed to a middle of the furnace, and a cold-face end surface which has a hexagonal shape larger than the hot-face end surface, the cold-face end surface being arranged at an outer periphery side of the furnace; the refractory blocks are arranged such that each position of the hot-face end surface is positioned along the radial direction of the furnace at a predetermined reference position; and the refractory blocks are arrayed along the circumferential direction of an inside surface of the steel shell, thereby being stacked in a honeycomb manner.

Abstract: A load transport mechanism for moving a heat treating load in a multi-station heat treating system is disclosed. The transport mechanism has a compact construction that allows it to fit in a centrally located stationary transport chamber. The transport chamber is adapted to provide ready access to multiple treating chambers arrayed around the chamber. The transport mechanism includes a load translation mechanism for moving the load linearly and a load rotation mechanism for rotating the load within the transport chamber. A multi-station heat treating system having a centrally located quenching chamber that includes the load transport mechanism is also disclosed.

Abstract: A method of forming a workpiece having an initial heat treatment and made of a naturally aging alloy to a final shape using an incremental sheet forming (ISF) machine having a coordinate system and a tool path corresponding to the final shape of the workpiece is disclosed. The method comprises positioning the workpiece in the ISF machine; performing an initial forming operation on the workpiece using the ISF machine; performing a final heat treatment on the workpiece; repositioning the workpiece in the ISF machine; and, with the workpiece in a final workpiece orientation in the ISF machine and the tool path of the ISF machine in a final tool-path orientation in the ISF machine, performing a final forming operation on the workpiece using the ISF machine to achieve the final shape of the workpiece. Intermediate heat treatments and intermediate forming operations in the ISF machine may also be performed.

Abstract: A steel sheet includes, by mass %: C: 0.020% to 0.080%; Si: 0.01% to 0.10%; Mn: 0.80% to 1.80%; and Al: more than 0.10% and less than 0.40%; and further includes: Nb: 0.005% to 0.095%; and Ti: 0.005% to 0.095%, in which a total amount of Nb and Ti is 0.030% to 0.100%, and the steel sheet includes, as a metallographic structure, ferrite, bainite, and other phases, an area fraction of the ferrite is 80% to 95%, an area fraction of the bainite is 5% to 20%, a total fraction of the other phases is less than 3%, a tensile strength is 590 MPa or more, and a fatigue strength ratio as a fatigue strength to the tensile strength is 0.45 or more.

Abstract: A method includes the introduction of a first medium into the compartment through an inlet and the heating of the first medium when it is present in the inlet. The heating takes place through the use of a combustion arrangement that is arranged in the inlet and that comprises fuel. The heating, the use of the combustion arrangement, includes in turn the ignition of the fuel, combustion of the fuel, and the transfer of the combustion heat to the first medium that is present at the combustion arrangement in the inlet. The combustion arrangement, is arranged in a region in the inlet, which in turn is arranged outside of the direct passage of the first medium in and through the inlet, such that the ignition of the fuel, the combustion of the fuel and the transfer of combustion heat to the first medium take place in this region.

Abstract: This invention provides a method for shortening operation shutdown time of high pressure acid leach equipment in a hydrometallurgical process, wherein the high pressure acid leach equipment comprises (i) means to transfer an ore slurry into the high pressure acid leach equipment;(ii) means to increase temperature and pressure of an ore slurry before leaching; (iii) means to add sulfuric acid into the high pressure acid leach equipment and to leach the ore slurry to obtain a leached slurry at high temperature under high pressure; (iv) means to adjust the pressure of the leached slurry; and (v) means to discharge the leached from the high pressure acid leach equipment; wherein, upon operation shutdown of the high pressure acid leach equipment, the leached slurry is subjected to self-circulation inside the high pressure acid leach equipment.

Abstract: The present invention provides a titanium slab for hot rolling which can be fed into a general purpose hot-rolling mill for producing strip coil, without passage through a breakdown process such as blooming or a straightening process, and can further suppress surface defect occurrence of the hot-rolled strip coil, and a method of producing and a method of rolling the same, characterized in that in the cast titanium slab an angle ? formed by the crystal growth direction (solidification direction) from the surface layer toward the interior and a direction parallel to the slab casting direction (longitudinal direction) is 45 to 90°, and moreover, there is a surface layer structure of 10 mm or greater whose ? is 70 to 90°, and further characterized in that a crystal grain layer of 10 mm or greater is formed whose C-axis direction inclination of a titanium ? phase is, as viewed from the side of the slab to be hot rolled, in the range of 35 to 90° from the normal direction of the surface to be hot rolled.

Abstract: A composite metal where a phase of particles of solid solution is uniformly dispersed in a Cu phase, the solid solution containing a solid solution of a heat resistant element selected from Mo, W, Ta, Nb, V and Zr and Cr. The composite metal is provided to contain: 20-70% of Cu; 1.5-64% of Cr; and 6-76% of a heat resistant element by weight relative to the composite metal, wherein a remainder is comprised of inevitable impurities. In the composite metal, the particles of the solid solution, contained in the composite metal, are provided to have an average particle diameter of not larger than 20 ?m and to uniformly disperse in the Cu phase with an index of the dispersion state of not higher than 1.0.

Abstract: The present invention relates to an aluminum alloy product for use as a finstock material within brazed heat exchangers and, more particularly, to a finstock material having high strength and conductivity after brazing. The invention is an aluminum alloy finstock comprising the following composition in weight %: Fe ?0.8-1.25; Si ?0.8-1.25; Mn 0.70-1.50; Cu 0.05-0.50; Zn up to 2.5; other elements less than or equal to 0.05 each and less than or equal to 0.15 in total; and balance aluminum. The invention also relates to a method of making the finstock material.

Abstract: The present disclosure relates to a manufacturing method of high-strength flake graphite cast iron, the high-strength flake graphite cast iron manufactured by the method, and an engine body including the cast iron, and more particularly, to flake graphite cast iron and a manufacturing method thereof, wherein the flake graphite cast iron has a uniform graphite shape and low probability of forming chill and has high tensile strength of at least 350 MPa and excellent workability and fluidity by controlling the content of manganese (Mn) and a trace of strontium (Sr), which are included in the cast iron, within a specific ratio.

Abstract: A method of making a crawler shoe for a mobile machine having a track assembly is disclosed. The method may include casting a crawler shoe from manganese steel, the crawler shoe having at least one wear surface that engages the track assembly. The method may further include solution treating the crawler shoe to form fully austenitic manganese steel, applying an explosive material to the at least one wear surface, and detonating the explosive material.

Abstract: Mixed magnetic powders for making a magnetic core or body is disclosed, wherein the mixed magnetic powders comprises: a first magnetic powder; a second magnetic powder, wherein the first magnetic powder and the second magnetic powder are made of a same soft magnetic material, wherein the ratio of the D50 of the first magnetic powder to the D50 of the second magnetic powder is in the range of 5 to 12, wherein the first magnetic powder weighs 50 to 90 percent of the total weight of the first magnetic powder and the second magnetic powder; and the second magnetic powder weighs 10 to 50 percent of the total weight of the first magnetic powder and the second magnetic powder.

Abstract: An austenitic antibacterial stainless steel formulation provides a high strength, highly corrosion resistant, antimicrobial product at a relatively low cost wherein antimicrobial performance is dramatic and greater mechanical properties and corrosion resistance are achieved as well. The alloy may comprise key constituents of Fe, Cr, Ni, and C plus a mischmetal having Ce and La components.

Abstract: A method for producing a motor vehicle component includes the steps of providing a precipitation-hardenable blank composed of a 6000 or 7000 grade aluminum alloy, solution-annealing the blank at a temperature between 350° C. and 550° C. for a time period of 2 to 30 min., in particular 3 to 20 min. and preferably 5 to 15 min., in particular at a temperature between 440° C. and 480° C. in the case of a 7000 grade aluminum alloy, and in particular at a temperature between 490° C. and 545° C. in the case of a 6000 grade aluminum alloy, subjecting the solution-annealed blank to partially different quenching, a first region being quenched to a temperature between 150° C. and 250° C., and a further region being quenched to a temperature below 150° C., deforming the blank during or after the partially different quenching.

Abstract: A method for hot-dip coating of a steel strip running in a bath of liquid metal such as zinc, or metal alloy contained in a pan is provided. Dross which are formed during the coating and float at the surface of the bath are moved away from the surface of the strip by at least one inductor. Each inductor produces a sliding electromagnetic field oriented along a given direction and generates a magnetomotive force, and the magnetomotive forces displaced the dross towards a container intended to collect them and/or towards an area of the surface of the bath from which they are discharged. For at least one of the inductors, the direction of the respective sliding electromagnetic field is reversed intermittently so as to modify the flows of the dross inside the pan. A hot dip coating facility is also provided.

Abstract: A method of manufacturing a deposition mask is disclosed. In one aspect, the method includes depositing a first photoresist layer on a substrate, aligning a first photomask over the first photoresist layer and developing the first photoresist layer to form a plurality of first photoresist patterns having sides that gradually narrow toward the substrate. The method also includes forming a metal layer over the first photoresist patterns and a portion of the substrate exposed by the first photoresist patterns, depositing a second photoresist layer over the metal layer and aligning a second photomask over the second photoresist layer and developing the second photoresist layer to form a plurality of second photoresist patterns between the first photoresist patterns. The method further includes etching the metal layer to form a pattern hole, removing the first and second photoresist patterns and separating the substrate so as to form a deposition mask.

Abstract: An amount of nitrogen in a compound film is controlled. A method of manufacturing compound film comprising forming films laminated on a substrate placed at a film forming chamber is provided. According to the method of manufacturing compound film, a first compound layer including one or more elements selected from metal elements and semimetal elements and oxygen element and a second compound layer including one or more elements and nitrogen element are laminated alternately. The first compound layer is formed by a Filtered Arc Ion Plating method and the second compound layer is formed by a sputtering method.

Abstract: A method is provided for manufacturing ceramic glass, including sapphire glass, for use in display covers in smartphones, computers, and watches, as well as for use as substrates on which semiconductor films can be deposited for a wide range of electronic applications, including solar cells, LEDs, and FETs.

Abstract: Methods and related apparatus support a work piece during a physical vapor deposition. An aluminium support having a support surface coated with a heat absorbent coating is provided. The support is cooled to around 100° C. and a PVD process is performed such that, with cooling, the work piece temperature is between 350° C. and 450° C. The coating is inert and/or ultra-high voltage compatible.

Abstract: Methods of depositing Co-containing layers on substrates are disclosed. The vapor of a Co-containing film forming composition is introduced into a reactor having a substrate disposed therein. The Co-containing film forming compositions comprise a silylamide-containing precursor selected from Co[N(SiMe3)2]2(NMe2Et), Co[N(SiMe3)2]2(NMeEt2), or combinations thereof. At least part of the silylamide-containing precursor is deposited onto the substrate to form the Co-containing layer using a vapor deposition method.

Abstract: Disclosed are devices, materials, systems, and methods, including a device that includes one or more structural components, at least one of the one or more structural components comprising substantially HfO2—TiO2 material. Also disclosed is a hemispherical resonator that includes a hemisphere including one or more structural components with at least one of the one or more structural components comprising substantially HfO2—TiO2 material, a forcer electrode configured to apply an electrical force on the hemisphere to cause the hemisphere to oscillate, and one or more sensor electrodes disposed in proximity to the hemisphere and configured to sense an orientation of a vibration pattern of the hemispherical resonator gyroscope.

Abstract: Electronic device fabrication processes, apparatuses and systems for flowable gap fill or flowable deposition techniques are described. In some implementations, a semiconductor fabrication chamber is described which is configured to maintain a semiconductor wafer at a temperature near 0° C. while maintaining most other components within the fabrication chamber at temperatures on the order of 5-10° C. or higher than the wafer temperature.

Abstract: Block copolymers (BCPs) and synthetic infiltration synthesis (SIS) are used to double the line density on a substrate. The BCP comprises first and second interconnected BCP components with a functional group at the junction or interface of the components. After deposition of the BCP on the substrate and annealing, a pattern of parallel stripes of first and second BCP components is formed with a pattern of functional group interfaces between the components. Each of the BCP components is non-reactive with atomic layer deposition (ALD) precursors, while the functional group is reactive with the ALD precursors. The ALD results in the infiltration of inorganic material into the interfaces where the reactive functional groups are located but without affecting the BCP components. After removal of the organic material, a pattern of parallel lines of inorganic material remains with a pitch half that of the stripes of BCP components.

Abstract: A roll-to-roll electroless plating system including a sump and a pan containing a plating solution. A web advance system advances a web of substrate though the plating solution in the pan along a web advance direction, wherein a plating substance in the plating solution is plated onto predetermined locations on a surface of the web of substrate. A pan-replenishing pump moves plating solution from the sump to an inlet of the pan through a pipe connected to an outlet of the pan-replenishing pump, the inlet of the pan being located below the web of substrate. A spreader duct includes a channel that is in fluidic communication with the inlet of the pan, wherein the channel is positioned below the web of substrate and includes at least one outlet disposed beyond the first edge or the second edge of the web of substrate.

Abstract: The present invention relates to a method for treating a metal surface, comprising (A) providing an ionic liquid solution and a substrate of a first metal, wherein the ionic liquid solution comprises an ionic liquid and an ion of a second metal; and (B) immersing the substrate of the first metal in the ionic liquid solution to form a coating layer of the second metal on a surface of the substrate of the first metal by reducing the ion of the second metal. The surface of the substrate of the first metal is protected by the coating layer of the second metal, thereby improving the corrosion resistance.

Abstract: Coated cutting implements having increased longevity, corrosion and stain resistance, a smooth and uniform appearance and color, and/or reduced friction between cutting blades are provided. The coatings on the cutting implements have at least two layers. The first layer is a metal-based layer that imparts hardness or wear-resistance to the cutting implement. The second layer is comprised of an organic polymer.

Abstract: A composite article includes a substrate and a powder-derived composite coating on the substrate. The composite coating includes discrete regions of a first material and discrete regions of a second material. At least one of the first material or the second material is a chemical precursor.

Abstract: Cutting tools are described having coatings which can demonstrate desirable wear resistance and increased cutting lifetimes. A coated cutting tool described herein has a substrate and a coating with a plurality of alternating layers of a first layer of Al2O3 and a second layer of at least one of MeAl2O3 and MeAl2O3/MeO2 composite, wherein Me is Zr, Hf, Ti or a combination thereof. The coating has a superlattice-like structure.

Abstract: Thermal barrier materials are provided that possess low heat capacity and low thermal conductivity, while at the same time, high structural integrity and robustness. In some embodiments, the disclosed coating comprises metal-containing spheres that are sintered or glued together and/or embedded in a matrix. The coating has at least 60% void volume fraction and closed porosity. The coating thickness is from 50 microns to 500 microns, and the metal spheres have an average diameter that is from about 5% to about 30% of the coating thickness. In some embodiments, the metal spheres have an average diameter that is 4-10 times smaller than the coating thickness. Thermal barrier materials with these coatings can be beneficial in engine applications, for example.

Abstract: An etching chamber is equipped with an actively-cooled element preferentially adsorbs volatile compounds that are evolved from a polymeric layer of a wafer during etching, which compounds will act as contaminants if re-deposited on the wafer, for example on exposed metal contact portions where they may interfere with subsequent deposition of metal contact layers. In desirable embodiments, a getter sublimation pump is also provided in the etching chamber as a source of getter material. Methods of etching in such a chamber are also disclosed.