Abstract: The invention provides a fungal cell, such as a yeast cell, capable of growing in co-cultivation with Propionibacterium. Also provided are methods of producing such cells and fermentation processes using the fungal cell of the invention and Propionibacterium in co-cultivation. Such co-cultivation significantly reduces the chemical oxygen demand load of the waste fermentation broth.

Abstract: For amperometric determination of the hydrogen peroxide concentration in a fluid sample, different electrical voltage steps are applied to the electrodes. In this process, at least one voltage is suitable for measuring the hydrogen peroxide concentration in the fluid sample, and at least one additional voltage is suitable for measuring the concentration of a substance in the fluid sample that acts as an interfering substance during measurement of the hydrogen peroxide concentration.

Abstract: This invention discloses a reagent composition for a biosensor having high sensitivity which is capable of improving analysis linearity of an analyte such as glucose by reacting an oxidoreductase, a metal-containing complex, and Naphthol Green B, and minimizing blood necessary for measuring blood glucose since it is possible to detect a concentration of a small amount of the analyte, and a biosensor having the same. The reagent composition for a biosensor includes at least two kinds of electron transfer mediators including Naphthol Green B, and an enzyme.

Abstract: Methods for identifying patients with anemia, distinguishing thalassemia-trait anemia from iron-deficiency anemia, and identifying pre-anemic patients several weeks before anemia becomes clinically detectable. Also, methods for detecting blood doping in athletes and for optimizing therapy with erythropoiesis stimulating agents or iron supplementation. Computer-readable storage devices and systems, e.g., for use in the described methods.

Abstract: Methods, apparatuses, and systems for screening, analyzing and selecting microorganisms from complex heterogeneous communities, predicting and identifying functional relationships and interactions thereof, and synthesizing microbial ensembles based thereon are disclosed. Methods for identifying and determining the absolute cell count of microorganism types and strains, along with identifying the network relationships between active microorganisms and environmental parameters, are also disclosed.

Abstract: A bismuth-containing concentration agent for microorganisms is provided. Additionally, articles that include the concentration agent and methods of concentrating a microorganism using the concentration agent are provided.

Abstract: Provided herein are compositions and methods for identifying and detecting modulators of Ras protein conformational states through the use of second harmonic generation (SHG) technology. Also provided herein are methods for detecting a conformational changes in the three dimensional structure of a protein bound to a supported lipid bilayer.

Abstract: Provided is a method for diagnosing cancer through a difference with a control group in view of the ratio of a deglycosylated peptide fragment and a non-glycosylated peptide fragment in a protein including an N-linked glycosylation motif. Further provided is a method for diagnosing cancer through the detection of the glycosylation ratio in the protein according to the subject matter enables the diagnosis of cancer with high specificity and sensitivity using at least one existing marker, and can be useful in discovering new markers for diagnosing cancer.

Abstract: Conjugates of 2,3-dihydroxynaphthalene and its derivatives with enzyme cleavable groups are chromogenic substrates that form colored compounds when complexed with metal ions, e.g. iron ions, on cleavage by enzymes, and are useful in microbial detection and identification. The cleavage products form purple or red-brown colored complexes, that can easily be observed by the naked eye. Microbes can be grown in the presence of the substrates and the metal salts that provide the metal ion for complexing with the 2,3-dihydroxynaphthalene product. Substituents in the naphthalene ring may affect the solubility of the substrates and also the diffusibility and color of the metal complexes. Some of the substrates yield soluble complexes on cleavage and are of particular value in liquid growth media. Other substrates produce less soluble complexes that are more suitable for use in solid agar media. Methods of synthesizing the substrates are described.

Abstract: The invention relates to the field of biotechnology. The method for determining the spatial and temporal distribution of the activity of a proteolytic enzyme in an in vitro heterogeneous system, such as blood or blood plasma, involves the introduction of a luminescent, fluorogenic or chromogenic substrate into a sample with the subsequent release of a detectable tag as the proteolytic enzyme cleaves the substrate, and the recording of the optical characteristics of the sample, which makes it possible to assess the spatial and temporal distribution of the activity of the enzyme. The device for the implementation of the above method comprises an in vitro system, a means for illuminating the sample, a recording means and a control means. A method for diagnosing homeostatic imbalances according to a change in the spatial and temporal distribution of the activity of a proteolytic enzyme in a blood sample is also proposed.

Abstract: The present invention provides, among other things, imidazo[1,2-?]pyrazine derivatives including The derivatives are useful in any method which other coelenterazines have been used. For example, the derivatives may be used in a bioluminogenic method to detect the presence of certain compounds or molecules.

Abstract: The invention described herein provides reagents (e.g., kits), compositions, and methods for carrying out an unbiased genome-wide strategy to identify the functional targets for all ncRNAs.

Abstract: Ligation assays for detecting and profiling expression products at transcriptome scale.

Abstract: The present invention is directed to a method for detecting an interaction between a ribonucleic acid (RNA) molecule and a second molecule. This method involves providing a nucleic acid construct that contains a promoter sequence, a nucleotide sequence encoding the RNA molecule, and an RNA polymerase blocking site. The nucleotide sequence encoding the RNA molecule is transcribed in vitro to produce an RNA transcript corresponding to the RNA molecule. Transcription is halted by the RNA polymerase blocking site under conditions effective for the RNA transcript to remain tethered to the nucleic acid construct. The tethered RNA transcript is contacted with the second molecule and any interaction between the tethered RNA transcript and the second molecule is detecting and identified based on said contacting.

Abstract: Provided herein are methods for generation and amplification of a single-stranded DNA circle in a single reaction vessel from a linear DNA without any intervening purification steps. The single-stranded DNA circle is generated via a template-independent single-stranded DNA ligation. Whole-genome amplification of linear chromosomal DNA in a single tube using ligation-assisted DNA amplification is also provided.

Abstract: A method includes performing a nucleic acid amplification of a nucleic acid sample using a detection probe, wherein the nucleic acid amplification occurs over one or more interrogation periods, and, from the nucleic acid amplification, acquiring amplification data that indicates an amount of nucleic acid present for each of the one or more interrogation periods. The method also includes, based on the amplification data, determining a crosstalk correction value associated with a spectral neighbor to the probe to reduce spectral crosstalk from the spectral neighbor; and applying the crosstalk correction value to amplification data collected from multiplex nucleic acid amplifications of nucleic acid samples.

Abstract: Provided are methods and compositions for detecting the presence or amount of one or more target nucleic acids in a sample. Methods of the present invention include linking universal nucleic acid segments into a single molecule in a linking reaction dependent on a target nucleic acid of interest. A variety of universal segment linking strategies are provided, including preamplification by polymerase chain reaction, ligation-based strategies, reverse transcription and linear polymerase extension. Linking the universal segments into a single molecule generates a tagged target nucleic acid which is detected in a manner dependent on an intramolecular interaction between one universal segment and a second portion of the tagged target nucleic acid. In certain embodiments, the intramolecular interaction includes the formation of a hairpin having a stem between a universal segment at one end of the tagged target nucleic acid and a second universal segment at the opposite end of the tagged target nucleic acid.

Abstract: Manufacturing methods and compositions are described for production of self-contained microfluidic cartridge devices with on-board reagents for molecular biological testing. Sensitive reagents are stored in dry form without lyophilization or freezing, and reconstituted at the point of use with either a biological sample or a sample eluate at the point of use. Manufacturing methods include sheet and roll fabrication processes where the reagents are printed in place and sealed within individual microfluidic cartridges before gel vitrification.

Abstract: Systems and methods are provided for forming emulsions from an aqueous fluid and oil. The aqueous fluid-in-oil emulsions are useful for numerous molecular biology techniques. The system is configured to cyclically translate a chamber containing the emulsion components, along a cycle path, while maintaining the axial orientation of the chamber with respect to a horizontal plane, and without rotating the chamber.

Abstract: Various embodiments of the present disclosure generally relate to molecular biological protocols, equipment and reagents for the sequencing of target nucleic acid (DNA, RNA, cDNA, etc) molecules.

Abstract: The present invention relates to a method for detecting a first and/or a second target DNA sequence from a DNA library, differing in that a mutation generates/eliminates a restriction site for a restriction endonuclease, comprising the steps of: (a) providing the DNA library, in which each of the DNA sequences comprises a first sequence segment, a second sequence segment of genomic DNA as cleaved by the restriction endonuclease, and a third sequence segment reverse complementary to the union of said first sequence segment and 5? overhang, if any, of the restriction endonuclease; (b) amplifying the library of DNA sequences by PCR using: a first reverse primer which hybridizes to the 3? end region of the second sequence segment of the first or second target sequence positive strand; a second forward primer which hybridizes to the 3? end region of the second sequence segment of the first target sequence antipositive strand; a third forward primer comprising a first portion hybridizing to the 5? end region of the

Abstract: Methods for improved quality control of nucleic acid containing biological samples utilized in high-throughput situations such as biorepositories engaged in the collection, processing, storage, distribution and analysis of such samples are disclosed.

Abstract: The present invention provides methods and materials useful for determining metabolizer status. Embodiments of the present invention provide an approach using a genotyping panel and integration of genotypes of CYP3A4 and CYP3A5 to assess CYP3A metabolizer status, applicable to all CYP3A substrates, including approximately 40% of all drugs. Algorithms for CYP3A metabolizer status are described. Where the contribution ratios of CYP3A4 and CYP3A5 to overall drug levels or drug effects are known, the algorithm can be used to calculate optimal dosing. Where the contributory ratios to overall drug effects are not available, the contributory ratios can be calculated with use of the genotypes for use in drug development. Embodiments of the present invention can be used in optimizing drug treatments, selecting dose, designing therapeutics, and predicting efficacy.

Abstract: A method of conjugating a substrate includes exchanging a counter ion associated with a biomolecule with a lipophilic counter ion to form a biomolecule complex, dispersing the biomolecule complex in a nonaqueous solvent, and coupling the biomolecule complex to a substrate in the presence of the nonaqueous solvent.

Abstract: The present disclosure generally pertains to a multiplex method for analyzing samples comprising using polynucleotide amplification to produce amplified products wherein one or more target sequences are tagged with a non-interfering, non-canceling target-specific polynucleotide identification tag, pyrosequencing the amplified products through the non-canceling target-specific polynucleotide identification tag sequence to detect the presence of one or more specific polynucleotide identification tags. The presence of a specific polynucleotide identification tag being correlated with the presence of a specific target sequence.

Abstract: Methods are provided for monitoring a subject having a graft for an acute rejection (AR) response, e.g., to predict, to diagnose, and/or to characterize an AR response. In practicing the subject methods, the expression level of at least one gene in a sample from the subject, e.g., a blood or biopsy sample, is evaluated, e.g., at the nucleic acid and/or protein level, to monitor the subject. Also provided are compositions, systems, kits and computer program products that find use in practicing the subject methods.

Abstract: The present invention relates to the use of DNA methylation profiles of patient samples for the diagnosis, prognosis and/or therapy monitoring of a heart disease in a patient, wherein the DNA methylation profile of the patient sample is compared with the DNA methylation profile of a control sample, and wherein a difference in the DNA methylation profile of the patient sample compared to the control sample is indicative of a heart disease or of the risk for developing a heart disease or for a prediction of therapy effects or therapy outcome. The present invention further relates to methods for the diagnosis, prognosis and/or therapy monitoring of a heart disease in a patient, comprising determining the DNA methylation profile in a patient sample comprising genomic DNA from heart cells, heart tissue or peripheral blood; and comparing the DNA methylation profile in the patient sample with the DNA methylation profile from a normal subject not having a heart disease or having a normal heart function.

Abstract: The present invention relates to a real-time PCR primer pair and probe for diagnosing Avellino corneal dystrophy, and more particularly to a real-time PCR primer pair and probe for diagnosing Avellino corneal dystrophy, which can accurately diagnose the presence or absence of a mutation in exon 4 of BIGH3 gene, which is responsible for Avellino corneal dystrophy. The use of the primer pair and probe according to the invention can diagnose Avellino corneal dystrophy in a more rapid and accurate manner than a conventional method that uses a DNA chip or PCR.

Abstract: The present invention relates to compositions and methods for cancer diagnosis, research and therapy, including but not limited to, cancer markers. In particular, the present invention relates to recurrent gene fusions as diagnostic markers and clinical targets for prostate cancer.

Abstract: The methods described herein allow for the classification of patients into groups for receiving optimized radiation treatment based on patient specific biomarker signature. The biomarker signature includes markers that have been shown to correlate with TGF-? expression and to be associated with tumor aggressiveness, radioresistance and poor prognosis. The markers play a key role in the epithelial-mesenchymal transition. The methods described herein provide the dual benefits of anti-tumor efficacy plus normal tissue protection when combining TGF-? inhibitors with ionizing radiation to treat cancer patients.

Abstract: Provided are oligonucleotides that are capable of detecting KRAS and PIK3CA mutations in both cancer patients and healthy individuals with high specificity in kPCR assays. When the oligonucleotides are used as forward primers in conjunction with a defined genotyping algorithm spreadsheet, the primers are capable of enhancing detection of KRAS codon 12, 13, and 61 and PIK3CA codon 542, 545, and 1047 single nucleotide polymorphisms (SNPs) in a background of wild-type sequences. The oligonucleotides of the present invention are also capable of preventing pseudogene amplification when the oligonucleotides are hybridized as reverse primers or detection probes to the mismatch sequences.

Abstract: The present invention provides a method for screening for iPS cells exhibiting differentiation resistance using a marker identified as lincRNA or mRNA that is specifically expressed in an iPS cell line exhibiting differentiation resistance, and such markers.

Abstract: Methods for distinguishing between cotton species by analyzing a sample of mature cotton fibers from raw cotton materials or from textile goods are disclosed. DNA is extracted from the mature cotton fiber sample and subjected to PCR techniques which enable the identification of the species of cotton utilized in the textile or cotton material of interest.

Abstract: In one aspect, the disclosure provides methods, compositions, vectors and kits for determining DNA repair capacity in cells or a subject.

Abstract: Methods and compositions for detection of enterovirus D in a sample, particularly detection of enterovirus D68, are provided. The methods include contacting a sample with at least one primer (such as a forward primer and/or a reverse primer) capable of specifically amplifying an EV-D68 viral protein 1 (VP1) nucleic acid or a portion thereof and/or a detectably labeled probe capable of specifically hybridizing to an EV-D68 VP1 nucleic acid, under conditions sufficient for specific amplification of the EV-D68 VP1 nucleic acid by the at least one primer and/or under conditions sufficient for specific hybridization of the probe to the EV-D68 nucleic acid. The amplification of the EV-D68 VP1 nucleic acid and/or the hybridization of the probe to the EV-D68 VP1 nucleic acid is detected, thereby identifying presence of EV-D68 in the sample.

Abstract: Provided herein are compositions, methods, and kits for detecting human Pegivirus 2 (HPgV-2). In certain embodiments, provided herein are HPgV-2 specific nucleic acid probes and primers, and methods for detecting HPgV-2 nucleic acid. In other embodiments, provided herein are HPgV-2 immunogenic composition compositions, methods of treating a subject with immunogenic HPgV-2 peptides, and methods of detecting HPgV-2 subject antibodies in a sample.

Abstract: The invention provides chimeric capture probes immobilizable via an L-nucleic acid tail that can bind to a complementary L-nucleic acid in an immobilized probe. The capture probes are useful for capturing a target nucleic acid from a sample. The L-nucleic acid in the tail of the capture probe bind to the complementary L-nucleic acid in the immobilized probe with similar affinity as would otherwise equivalent D-nucleic acids. However, the L-nucleic acid of the capture probe tail and immobilized probes do not form stable duplexes with D-nucleic acids present in the sample containing the target nucleic acid. Binding of nucleic acids in the sample directly to immobilized probe or to the tail of the capture probe is reduced or eliminated increasing the sensitivity and/or specificity of the assay.

Abstract: The present application discloses a method for carbonation with CO2. The method now disclosed describes the use of a static or dynamic mixer to react the CO2 with the incoming of nation liquor to whom Ca(OH)2 was previously added and readily starts the precipitation of tiny carbonate crystals. This solution can be advantageously used to compensate the deficit of CO2 in the carbonation process. This method for carbonation can be applied for example in the sugar refining industry.

Abstract: A liquid smoke tanning method uses liquid smoke as a tanning agent for tanning an animal skin, such as a hide or fur. The liquid smoke is used a natural source of aldehydes, which are commonly used in the tanning process and known to introduce toxicity into the animal skin. After the skin is prepared and cleaned for tanning, it is tanned in a liquid smoke solution, which may also include alum in an alternative embodiment. The liquid smoke is efficacious for crosslinking collagen proteins in the animal skin during the tanning process. The liquid smoke provides substantially the same collagen crosslinking capacity of aldehydes such as glutaraldehyde, formaldehyde, or glyoxol. The liquid smoke is, however, organic and does not contain the level of toxins found in chemically produced aldehydes. After the tanning, the skin is dried through known means for production as an organic, nontoxic leather.

Abstract: A method is provided for operating a blast furnace by blowing at least a solid reducing material and a combustible gas into the furnace through tuyeres with a lance inserted into a blowpipe, wherein a tube-bundle type lance obtained by bundling a plurality of blowing tubes is used and when only a solid reducing material or two kinds of a solid reducing material and a combustible gas or three kinds of a solid reducing material, a combustible gas and a gaseous reducing material is simultaneously blown into an inside of the blast furnace through a tube for blowing the solid reducing material, a tube for blowing the combustible gas and a tube for blowing the gaseous reducing material in the tube-bundle type lance, two or more tube-bundle type lances are inserted into the blowpipe to approximate their front ends to each other and blowing is performed so that the respective blowout streams interfere with each other in the blowpipe.

Abstract: A process for producing reducing gas for use in the production of direct reduced iron (DRI) and fuel gas for use in a steel mill, including: compressing a coke oven gas (COG) stream in a compressor; passing the compressed coke oven gas stream through an activated charcoal bed to remove tars from the compressed coke oven gas stream; separating a hydrogen-rich gas stream from the compressed cleaned coke oven gas stream using a pressure swing absorption unit; providing the hydrogen-rich gas stream to a direct reduction shaft furnace as reducing gas; and providing a remaining gas stream from the pressure swing absorption unit to a steel mill as fuel gas. Both once-through and recycle options are presented. Optionally, basic oxygen furnace gas (BOFG) is added to the reducing gas.

Abstract: A direct reduction process producing DRI from iron oxide particles by reduction at a about 750° C. with a reducing gas mainly H2 and CO, that also includes CO2, H20, and methane, a the reduction reactor and the top gas effluent from the reduction reaction after cooling/scrubbing is split. The resulting first top gas portion with a first hydrocarbon-containing make-up gas passes through a catalytic reformer yielding an improved hot reducing gas first effluent. The second top gas portion passes through a CO2 removal unit and then with the second hydrocarbon-containing make-up gas passes through a heater yielding a hot CO2-lean recycle reducing gas second effluent. The first and second effluents are fed to the reducing zone of the reduction reactor as the reducing gas reactant. The flow rate of at least the second of the two make-up gases is regulated to control the carbon content of the DRI produced.

Abstract: A turbine induction temper system. In one embodiment, an induction temper system for a turbine includes: an induction member; a control system operably connected to the induction coil; and a temperature sensor operably connected with the control system, wherein the control system is configured to control an electrical current supplied induction member in response to a temperature indicator about a component of the turbine obtained from the temperature sensor.

Abstract: In the present invention, a press-formed product is manufactured by heating a steel sheet for hot pressing use to a temperature of 900° C. or above and 1,100° C. or below, the steel sheet for hot pressing use having a predetermined chemical component composition, some of Ti-containing precipitates contained in the steel sheet, each of which having an equivalent circle diameter of 30 nm or less, having an average equivalent circle diameter of 6 nm or less, and the precipitated Ti amount and the total Ti amount in the steel fulfilling the relationship represented by formula (1) shown below, thereafter starting press-forming, and holding at the bottom dead point and cooling to a temperature lower than the martensite transformation starting temperature Ms while securing the average cooling rate of 20° C./s or more within a tool. Precipitated Ti amount(mass %)?3.4[N]<0.5×[total Ti amount (mass %)?3.4[N]]??(1) (In the formula (1), [N] represents the content (mass %) of N in the steel.).

Abstract: The present invention provides a low-alloy high-purity ferritic stainless steel sheet provided with improved oxidation resistance and high-temperature strength by utilizing Sn addition in trace amounts without relying on excessive alloying of Al and Si which reduces fabricability and weldability or addition of rare elements such as Nb, Mo, W, and rare earths, and a process for producing the same. The high-purity ferritic stainless steel sheet includes C: 0.001 to 0.03%, Si: 0.01 to 2%, Mn: 0.01 to 1.5%, P: 0.005 to 0.05%, S: 0.0001 to 0.01%, Cr: 16 to 30%, N: 0.001 to 0.03%, Al: 0.05 to 3%, and Sn: 0.01 to 1% (% by mass), with the remainder being Fe and unavoidable impurities. A stainless steel slab having such steel components is heated, wherein an extraction temperature is 1100 to 1250° C., and a winding temperature after hot rolling is 650° C. or lower. A hot-rolled sheet is annealed at 900 to 1050° C., and cooled at 10° C./sec or less over a temperature range of 550 to 850° C.

Abstract: An abrasion resistant steel plate or steel sheet suitable for use in construction machines, industrial machines, and the like and a method for manufacturing the same. In particular, a steel plate or steel sheet has a composition containing 0.20% to 0.30% C, 0.05% to 1.0% Si, 0.40% to 1.20% Mn, P, S, 0.1% or less Al, 0.01% or less N, and 0.0003% to 0.0030% B on a mass basis, the composition further containing one or more of Cr, Mo, and W, the composition further containing one or more of Nb, Ti, Cu, Ni, V, an REM, Ca, and Mg as required, the remainder being Fe and inevitable impurities. A semi-finished product having the above steel composition is heated, hot rolling is performed, air cooling is performed, reheating is performed, and accelerated cooling is then performed or accelerated cooling is performed immediately after hot rolling.

Abstract: A hardened formed part is manufactured with the steps: producing a blank from a hardenable strip material; heating of the blank to an austenitization temperature; forming and hardening of the blank to a hardened formed part; cleaning the hardened formed part; coating the hardened formed part with a metallic coating in an dipping bath with an electrolyte solution, wherein during the coating process, at least one auxiliary element is used in the dipping bath, such, that the deposition of the coating is partially influenced. A plant is used for manufacturing a hardened formed part.

Abstract: A method of forming a golf club head assembly includes aligning a faceplate with a recess of a club head; welding the faceplate to the club head; then, after welding the faceplate, heating the club head and the faceplate to at least a solvus temperature of the faceplate for a predetermined amount of time; and then, after heating the club head and the faceplate, allowing the club head and the faceplate to air cool.

Abstract: A method of producing a metal pipe which can suppress quenching defects is provided. In the method, a bend of the metal pipe is straightened by a straightening machine 10. Next, both pipe end portions of the metal pipe whose bend has been straightened are cut off by a pipe cutting apparatus 20. Next, a plurality of the metal pipes whose both pipe end portions have been cut off are aligned in the axial direction thereof to be conveyed to a quenching apparatus 30, and the metal pipes are quenched by being cooled after being heated by induction heating.

Abstract: The present invention relates to new compositions of matter, particularly metals and alloys, and methods of making such compositions. The new compositions of matter exhibit long-range ordering and unique electronic character.