Abstract: The present invention relates to recombinant filamentous fungal host cells producing cellulolytic enzyme compositions and methods of producing and using the compositions.

Abstract: The present invention provides methods of amplifying a target nucleic acid utilizing a circularized template. Circularization may be achieved utilizing a bridging oligonucleotide or an inverter primer. The bridging oligonucleotide or inverted primer is extended forming a concatemeric amplicon that can then be used as a template to provide exponential amplification of the target nucleic acid.

Abstract: Recombinant polypeptides having UDP-glycosyltransferase activities, including a 1,2-19-O-glucose glycosylation activity and a 1,2-13-O-glucose glycosylation activity for synthesizing of steviol glucosides, are provided. A method of producing a steviol glycoside composition using such recombinant polypeptide is also provided. Also disclosed are steviol glycosides referred to as rebaudioside Z1 and rebaudioside Z2.

Abstract: Methods, compositions and kits for selectively altering and detecting modified cytosine residues are provided.

Abstract: A microfluidic apparatus, method, and associated applications utilize and apply to a formalin-fixed paraffin-embedded (FFPE) tissue sample and performing a liquid-liquid extraction to remove the paraffin from the tissue sample prior to a nucleic acid purification step. A microfluidic device includes a dedicated liquid-liquid extraction process vessel, a nucleic acid purification process component, and a nucleic acid amplification reactor. A liquid-liquid extraction and nucleic acid purification kit includes a microfluidic device capable of performing both a liquid-liquid extraction process and a nucleic acid purification process, including a dedicated liquid-liquid extraction process vessel, an immiscible liquid or a precursor phase thereof disposed in the vessel, a nucleic acid purification process component, a nucleic acid amplification reactor fluidically, and a supply of reagents suitable to enable the liquid-liquid extraction process and the nucleic acid purification process.

Abstract: Provided herein are methods of detecting nucleic acids. The nucleic acid of interest may be detected by using Cas endonuclease to degrade substantially all nucleic acid in a sample except for the nucleic acid of interest, leaving the nucleic acid of interest isolated and amenable to detection. In related methods, Cas endonuclease complexes are used to protect the nucleic acid of interest while unprotected nucleic acid is digested, e.g., by exonuclease, after which the isolated nucleic acid of interest is detected.

Abstract: [Object] A method of detecting nucleic acids easily without requiring complicated operations such as mixing of liquids and cleaning within micro-scale flow channels. [Solving Means]A method of detecting nucleic acids including the steps of bringing a sample containing the nucleic acids into contact with copper, and detecting fluorescence emitted from the sample is provided. According to the method of detecting nucleic acids, only by bringing the sample containing nucleic acids into contact with copper, the fluorescence derived from the composite of the nucleic acids and copper can be easily detected.

Abstract: A method is disclosed for calibrating a sensor element, which has an immobilized probe oligonucleotide, via which the bonding of a target nucleic acid (Z) can be detected by the sensor. In at least one embodiment, the method includes: a) bringing the sensor element into contact with a control nucleic acid (K), the melting temperature Tm(K) of which is less than the melting temperature Tm(Z) of the target nucleic acid (Z); b) hybridizing the control nucleic acid (K) to the probe oligonucleotide at a temperature T[p]<Tm(K), and detecting a positive control signal; and optionally c) modifying the stringent conditions such that T[n]>Tm(K) and detecting a negative control signal at a temperature T[n]. According to a refinement of at least one embodiment of the invention, a measuring signal of the target nucleic acid (Z) is measured at a measuring temperature T [mess], where Tm(K)<T[mess]<Tm(Z). The method is suited in particular for the calibration and quality control of microarrays.

Abstract: The present invention relates to methods for the design and/or production of a probe or primer that is capable of hybridizing to a plurality of sites in a sample comprising nucleic acid. Furthermore, the present invention provides methods for detecting and amplifying nucleic acid using such a probe or primer, for example, for identification of a strain, species or genera. Probe or primer sequences are determined by reference to codon usage bias of a target nucleic acid. In addition, the present invention provides methods for determining codon distribution and/or base pair distance between codons in a nucleic acid.

Abstract: In some embodiments, the present inventions relates generally to compositions, methods and kits for use in discriminating sequence variation between different alleles. More specifically, in some embodiments, the present invention provides for compositions, methods and kits for quantitating rare (e.g., mutant) allelic variants, such as SNPs, or nucleotide (NT) insertions or deletions, in samples comprising abundant (e.g., wild type) allelic variants with high specificity and selectivity. In particular, in some embodiments, the invention relates to a highly selective method for mutation detection referred to as competitive allele-specific TaqMan PCR (“cast-PCR”).

Abstract: The present invention is directed to sequencing of nucleic acids. A method is provided for sequencing based on immobilized nucleic acid on a surface. Advantageously, a long range detection mechanism is used for detecting, whether a nucleotide provided to the substrate of a biochip has been incorporated into the immobilized template nucleic acid. Various different alignment means are provided by the present invention which can be used for facilitating a rigidly locking of the orientation of the DNA complex, which complex comprises the template nucleic acid, the primer and the capture nucleic acid. Various different linker systems may be used to immobilize the DNA complex at a first and a second strand end, such that the desired alignment of the DNA complex is achieved. Also co-adsorbed molecules on the substrate surface can be used for such an aligning measure.

Abstract: Devices and methods that can detect and control an individual polymer in a mixture is acted upon by another compound, for example, an enzyme, in a nanopore are provided. The devices and methods also determine (˜>50 Hz) the nucleotide base sequence of a polynucleotide under feedback control or using signals generated by the interactions between the polynucleotide and the nanopore. The invention is of particular use in the fields of molecular biology, structural biology, cell biology, molecular switches, molecular circuits, and molecular computational devices, and the manufacture thereof.

Abstract: Methods, compositions, and systems are provided that allow for reliable sequencing of the initial sequence region of a sequence of interest. The methods of the invention allow for more reliable barcoding of subpopulations of nucleic acids to be sequenced.

Abstract: In some embodiments, methods for obtaining sequence information from a nucleic acid template linked to a support include hybridizing a first primer to a template strand linked to a support, sequencing a portion of the nucleic acid template, thereby forming an extended first primer product that is complementary to a portion of the nucleic acid template, In some embodiments, the method further includes introducing a nick into a portion of the template strand that is hybridized to the extended first primer product, degrading a portion of the template strand from the nick using a degrading agent, where a portion of the extended first primer remains hybridized to an undegraded portion of the template strand, and sequencing at least some of the single-stranded portion of the extended first primer by synthesis.

Abstract: The present invention relates to a set of biomarkers, microarrays that provide for detection thereof, an expression signature comprising 16 genes or a subset thereof, and the use thereof in determining the regulation status of IL-6/STAT3 signaling pathway in a cell sample or subject, as well as compositions for the detection thereof. The regulation status of IL-6/STAT3 signaling pathway in a cell sample or subject may be assayed based on the level of expression of one or more of these genes. The methods and compositions provided herein may be used to evaluate IL-6/STAT3 pathway regulation status in a sample; classify a cell sample as having a deregulated or regulated IL-6/STAT3 signaling pathway; determine whether an agent modulates the IL-6/STAT3 signaling pathway; predict the response of a subject to an agent that modulates the IL-6/STAT3 signaling pathway; assign treatment to a subject; and/or evaluate the pharmacodynamic effects of therapies designed to regulate IL-6/STAT3 pathway signaling.

Abstract: Disclosed herein is a system and method for increasing the fidelity of measured genetic data, for making allele calls, and for determining the state of aneuploidy, in one or a small set of cells, or from fragmentary DNA, where a limited quantity of genetic data is available. Poorly or incorrectly measured base pairs, missing alleles and missing regions are reconstructed using expected similarities between the target genome and the genome of genetically related individuals. In accordance with one embodiment, incomplete genetic data from an embryonic cell are reconstructed at a plurality of loci using the more complete genetic data from a larger sample of diploid cells from one or both parents, with or without haploid genetic data from one or both parents. In another embodiment, the chromosome copy number can be determined from the measured genetic data, with or without genetic information from one or both parents.

Abstract: The present invention relates to determining the receptivity of human endometrium from a gene expression profile. More specifically, the invention consists of developing a specific expression microarray of endometrial receptivity (Endometrial Receptivity Array or ERA) which allows evaluating the receptive state of a human endometrium, as well as assessing said state for diagnostic and therapeutic purposes.

Abstract: The invention provides tandem single nucleotide polymorphisms and methods for their use, for example, in diagnosing Down Syndrome.

Abstract: The present disclosure provides gene expression profiles that are associated with prostate cancer. The gene expression profiles can be used to detect prostate cancer cells in a sample and to distinguish between well differentiated (WD) prostate cancer and poorly differentiated (PD) prostate cancer. Also provided is an array comprising oligonucleotide probes for detecting the unique gene signature associated with WD and/or PD prostate cancer.

Abstract: Animal husbandry has always been susceptible to the ravages of pathogenic infections. Poultry flus and cattle diseases are but two examples that have dire consequences for animals and adversely affect the economic fortunes of farmers. A testing and culling methodology is presented that can eliminate pathogens from an infected herd. The sensitivity of PCR to detect very low levels of nucleic acid of an infecting pathogen is utilized to identify infected animals. In addition, it has been discovered that PCR analysis of manure samples can accurately identify infected animals and offspring for those species that consume placental remains after birth. Mink Aleutian Disease Virus (mADV) is one of several deadly DNA parvoviruses that can quickly reach epidemic proportions in a mink herd. PCR primers have been developed that generate amplicons to detect and identify the mADV. In addition, a previously unidentified strain of mADV has been discovered, genomically sequenced, and substantially detailed.

Abstract: Methods of detecting at least one genetic variation in a polynucleotide analyte in a sample. A fluorophore is attached to a first primer, a quencher is attached to a second primer, the first primer and the second primer are specific for the polynucleotide analyte. A signal generated by the fluorophore and quencher is measured. PCR is performed with the first primer and the second primer using the polynucleotide analyte as a template, thereby amplifying the template. A signal generated by the fluorophore and quencher from the PCR amplification product is measured. Comparison is made of the signals; and a determination is made of the presence or absence of the at least one genetic variation based i) on the change in signal as determined; and ii) by comparing said change to the change in signal observed upon PCR amplification for a corresponding polynucleotide analyte lacking the at least one genetic variation.

Abstract: A process for hardening tubulars and increasing their surface area for heat transfer can be performed in place in a borehole or on the surface. A pattern is applied to an interior wall with at laser, electron beam or radiation source that is remotely controlled to apply the hardening pattern to the inside or outside wall as inert gas or clean fluid is applied. Pressure differential is applied to the wall so that the non-hardened portions or the negative of the hardened pattern plastically or elastically deform to increase surface area and enhance load resistance of tubular or sheets. Alternatively, wall differential pressure is applied with an insert having a raised pattern on its exterior surface causing the spaces where the pattern is absent to plastically deform to enhance surface area. When done in a borehole annulus pressure or stand pipe pressure is applied or a vacuum is pulled inside the tubular to generate differential pressure for hydro-forming or switching dents in an opposite stable condition.

Abstract: A steel wire according to an aspect of the present invention includes a predetermined chemical composition, in which a wire diameter R of the steel wire is 1.0 mm to 3.5 mm, a soft portion is formed along an outer circumference of the steel wire, the Vickers hardness of the soft portion is lower than that of a position of the steel wire at a depth of ¼ of the wire diameter R by Hv 30 or higher, the thickness of the soft portion is 5 ?m to 0.1×R mm, the metallographic structure of a center portion of the steel wire contains 95% to 100% of pearlite by area %, the average lamellar spacing of pearlite in a portion from a surface of the steel wire to a depth of 5 ?m is less than that of pearlite at the center of the steel wire, the difference between the average lamellar spacing of pearlite in the portion from the surface of the steel wire to the depth of 5 ?m and the average lamellar spacing of pearlite at the center of the steel wire is 3 nm to 60 nm, and the tensile strength is 1100 MPa or higher.

Abstract: The invention provides a method for the production of a metal, the method comprising the steps of mixing an oxide of the metal with a reducing agent comprising a Group II metal or a hydride thereof in the presence of water and/or an organic solvent, heating the mixture of oxide and reducing agent, leaching the resulting material with water; and washing the leached material with a dilute aqueous acid. Typically, the metal is a transition or rare earth metal, the oxide of the metal is an oxide of a transition or rare earth metal, and the reducing agent is selected from calcium or magnesium or the hydrides of calcium and magnesium. The metal is generally obtained at a purity of around 98.5-99.1%, and the method is much quicker than the methods of the prior art and has a much lower carbon footprint, thereby providing an option which is more sustainable, environmentally friendly, and accommodative for industries.

Abstract: A method for the treatment of flue dusts containing arsenic and/or antimony from pyrometallurgical methods, wherein a reducing agent is added to the flue dusts, the flue dusts are heated together with the reducing agent, and volatile components are separated from a slag. The reducing agent is a carbonaceous compound.

Abstract: A method of operating a concentrator may comprise producing a final copper concentrate and periodically or continuously analyzing the produced final copper concentrate to obtain a grade value of the produced final copper concentrate. The produced final copper concentrate may be sent to a downstream smelting operation if/when the grade value of the produced final copper concentrate is at or above a minimum acceptable grade threshold and may be sent to a downstream hydrometallurgical operation capable of producing copper cathode, if/when the grade value of the produced final copper concentrate is below or falls below the minimum acceptable grade threshold.

Abstract: A cation adsorbent of an embodiment includes tungsten oxide particles having a BET specific surface area in a range of 0.82 m2/g or more and 820 m2/g or less. The cation adsorbent is added to a solution to be treated containing cations being recovery objects, and the cation adsorbent adsorbing the cations is precipitated. The generated precipitate is separated from the solution to recover the cations.

Abstract: Provided is a method for recovering scandium, with which it is possible to easily and efficiently recover high-purity scandium from nickel oxide ores. This method for recovering scandium involves passing a solution containing scandium through an ion exchange resin, then subjecting the eluant eluted from the ion exchange resin to solvent extraction and separating the extraction residual liquid and the extraction agent after extraction, then performing an oxalation process on the extraction residual liquid to obtain a scandium oxalate precipitate, and roasting the precipitate to obtain scandium oxide, wherein the method is characterized in that an amine-based extraction agent is used as the extraction agent for solvent extraction.

Abstract: A mixed mother alloy is prepared from a solder mixture comprising a pyrolyzable flux and high melting point metal particles, the mixed mother alloy is charged into a large amount of molten solder and stirred, and a billet is prepared. The billet can then be extruded, rolled, and punched to form a pellet or a washer, for example.

Abstract: A magnesium alloy is suitable for use as a corrodible downhole article, wherein the alloy includes: (a) 11-15 wt % Y, (b) 0.5-5 wt % in total of rare earth metals other than Y, (c) 0-1 wt % Zr, (d) 0.1-5 wt % Ni, and (e) at least 70 wt % Mg. It has been surprisingly found by the inventors that by increasing the Y content of the alloy to the range specified above, increased age hardening response and hence increased 0.2% proof stress can be achieved.

Abstract: Manufacturing a cold-rolled steel plate by smelting a steel slab having the chemical composition containing on the basis of percent by mass, C from 0.03 to 0.12%, Si from 0 to 1.0%, Mn from 0.2 to 0.8%, P at 0.03% or less, S at 0.03% or less, Ti from 0.04 to 0.3%, and Al at 0.05% or less, with a residue being formed of Fe and unavoidable impurities, the chemical composition satisfying 5*C %?Si %+Mn %?1.5 *Al %<1, and an average diameter of particles of a Ti-based carbide as a precipitate is from 20 to 100 nm; heating the slab to 1200° C. or more and hot rolling, forming a hot-rolled steel plate; winding the hot-rolled steel plate from 500 to 700° C. to form a hot-rolled coil; and cold rolling or annealing and cold rolling the coil obtaining cross-sectional hardness from 200 to 400 HV.

Abstract: The present invention is a heat-resistant Ni-base alloy including a Ni—Ir—Al—W alloy having essential additive elements of Ir, Al, and W added to Ni, wherein the heat-resistant Ni-base alloy includes Ir: 5.0 to 50.0 mass %, Al: 1.0 to 8.0 mass %, and W: 5.0 to 20.0 mass %, the balance being Ni, and a ?? phase having an L12 structure disperses in a matrix as an essential strengthening phase. The heat-resistant material including the Ni-base alloy may contain one or more additive elements selected from B: 0.001 to 0.1 mass %, Co: 5.0 to 20.0 mass %, Cr: 1.0 to 25.0 mass %, Ta: 1.0 to 10.0 mass %, Nb: 1.0 to 5.0 mass %, Ti: 1.0 to 5.0 mass %, V: 1.0 to 5.0 mass %, and Mo: 1.0 to 5.0 mass %, or 0.001 to 0.5 mass % of C.

Abstract: A method for the practice-oriented, operationally reliable production of castings of an AlCu alloy which consists of Cu:, Mn:, Zr:, Fe:, Si:, Ti:, V:, remainder Al and unavoidable impurities. A melt which has been melted according to this alloy formula is kept at temperature for several hours and then mixed vigorously at least once. Thereafter, the melt is cast in portions into the respective casting which is then solution annealed at temperature for several hours. The casting is quenched from the solution anneal temperature to a maximum temperature of 300° C., at a specified cooling rate which the casting passes through during quenching. The casting is then artificially aged for several hours at 150-300° C. Finally, the casting is cooled to room temperature.

Abstract: A metallic flat product is disclosed which is subjected to surface finishing by hot-dip coating and which is preferably composed of steel. The metallic flat product includes a metallic alloy layer (11) and a metallic surface layer (12?), which metallic alloy layer and metallic surface layer differ from one another in terms of their chemical composition. The two layers (11, 12?) are produced in a one process step and define a continuous transition region (13) in which a mixture of the two different chemical compositions is present. The metallic alloy layer (11) has a thickness of less than 8 ?m, preferably less than 6 ?m, and the surface layer (12?) is formed from aluminum or zinc. The flat product has an improved coating, by means of which the flat product more effectively satisfies the requirements with regard to good deformability and has good anti-corrosion protection.

Abstract: The invention pertains to the domain of metallurgy in particular, thermochemical surface treatment of products made of metals, mainly steels, and their alloys and it can be used for products hardening for the purpose of their service durability increase. The method of improvement of mechanical properties of products made of metals, mainly steels and alloys on their basis includes products nitriding in the gas atmosphere containing nitrogen and-or its compounds in the presence of a catalyst. Together the product and the catalyst are subject to hot isostatic pressing with observation of conditions of the barometric and temperature impact that provides achievement of dislocations density in the product's volume that satisfies conditions of transition of a part of the product substance into the positron state of the Dirac matter.

Abstract: A plate flatness adjusting device includes at least one tendon, an accommodation portion, and a tensile strength adjuster. The at least one tendon penetrates a through-hole of a plate and has lateral ends protruding outside of the plate. The accommodation portion accommodates the lateral ends of the at least one tendon. The tensile strength adjuster is coupled to the accommodation portion to adjust a tensile strength of the at least one tendon. The adjusted tensile strength adjusts a degree of flatness of the plate.

Abstract: The present disclosure relates to the field of display technology, particularly to a vacuum deposition apparatus and a vapor deposition method. The vacuum deposition apparatus includes a vacuum chamber and a rotary base, an evaporation source, and a plurality of vapor deposition zones arranged in series from bottom to top in the vacuum chamber, wherein the shape of the rotary base is a Reuleaux triangle, and the trajectories of movement of its vertices in the horizontal plane is a rounded square, the vapor deposition zones are arranged at intervals along the trajectories of movement of the vertices of the rotary base, the evaporation source is driven by the rotary base to pass below the vapor deposition zones sequentially, so that the evaporation source can be used to perform the vapor deposition operation in multiple directions simultaneously, thus improving the uniformity of film formation and utilization of the evaporation material.

Abstract: Methods for the selective processing of the outer portion of a workpiece are disclosed. The outer portion is processed by directing an ion beam toward the workpiece, where the ion beam extends beyond the outer edge of the workpiece at two locations. The workpiece is then rotated relative to the ion beam about the center so that all regions of the outer portion are exposed to the ion beam. The workpiece may be rotated an integral number of rotations. The ion beam may perform any process, such as ion implantation, etching or deposition. The outer portion may be an annular ring having an outer diameter equal to that of the workpiece and having a width of 1 to 30 millimeters. The rotation of the workpiece may be aligned with a notch on the outer edge of the workpiece.

Abstract: A deposition apparatus configured to perform a deposition process on a substrate, the deposition apparatus including a chamber having an exhaust opening in a surface, a deposition source in the chamber configured to eject one or more deposition materials toward the substrate, a cooling plate corresponding to an inner surface of the chamber, at which the exhaust opening is formed, a refrigerator contacting the cooling plate, and a pump coupled to the exhaust opening.

Abstract: A thin-film depositing apparatus including a mask, and a chucking unit for adhering the mask to a surface of a substrate, wherein the chucking unit includes a plurality of magnet units that contact another surface of the substrate by independently rising or falling by using their weight and thus are magnetically combined with the mask.

Abstract: An apparatus for treating the interior of containers includes a chamber for holding a container and provides precursor materials via an annulus formed by coaxially arranged electrodes at which plasma is created upon application of voltage and the container is treated.

Abstract: The present invention provides various passive electronic components comprising a layer of coated nanoparticles, and methods for producing and using the same. Some of the passive electronic components of the invention include, but are not limited to conductors, resistors, capacitors, piezoelectronic devices, inductors and transformers.

Abstract: An evaporation apparatus for depositing material on a substrate by a drum is described. The evaporation apparatus includes a first set of evaporation crucibles aligned in a first line a first direction for depositing evaporated material on the substrate; a first gas supply pipe extending in the first direction being arranged between at least one of the evaporation crucibles of the first set of evaporation crucibles and the drum; and a second gas supply pipe extending in the first direction for providing a gas between the first set of evaporation crucibles and the drum with openings shaped and positioned to improve the uniformity of the deposition of the material.

Abstract: A linear evaporation source and a deposition apparatus having the same are disclosed. In one embodiment, the linear evaporation source includes i) a crucible being open on one side thereof and configured to store a deposition material and ii) a plurality of partitions dividing an internal space of the crucible, wherein each of the partitions has at least one opening in a lower portion thereof. The source further includes i) a nozzle section located on the open side of the crucible and comprising a plurality of nozzles, ii) a heater configured to heat the crucible and iii) a housing configured to accommodate the crucible, the nozzle section, and the heater.

Abstract: Provided is a technology including a nozzle base end portion which is provided in a processing chamber processing a substrate to extend in a vertical direction and into which a processing gas processing the substrate is introduced, a nozzle distal end portion which is configured in a U shape and in which a gas supply hole supplying the processing gas is provided to a side surface of the substrate, and a gas residence suppressing hole which is provided in a downstream end of the nozzle distal end portion and has a diameter larger than that of the gas supply hole.

Abstract: A substrate processing system includes a processing chamber and an upper electrode arranged in the processing chamber. A pedestal is configured to support a substrate during processing and includes a lower electrode. An RF generating system is configured to generate RF plasma between the upper electrode and the lower electrode by supplying an RF voltage. A bias generating circuit is configured to selectively supply a DC bias voltage to one of the upper electrode and the lower electrode. A start of the DC bias voltage is initiated one of a first predetermined period before the RF plasma is extinguished and a second predetermined period after the RF plasma is extinguished. A substrate movement system is configured to move the substrate relative to the pedestal while the DC bias voltage is generated.

Abstract: There is disclosed a plasma enhanced chemical vapor deposition apparatus including a chamber in which plasma reaction is performed to provide a functional film to an object received therein, a pallet mechanically and electrically connected with the object, a conveyer to convey the pallet to an inside from an outside of the chamber, and a power supplier to supply an electric power to the pallet, the power supplier comprising a moving contact distant from the pallet when the pallet is conveyed and contacting with the pallet when the pallet is stopped.

Abstract: The present invention relates to a metal oxide layer structure formed on the surface of a substrate, in which the atomic numbers of the element metal and the element oxygen in the metal oxide layer are in a non-stoichiometric ratio, so that the metal oxide layer has a high density corresponding to 90-100% of the metal oxide before coating and is free of cracks and pores. The metal oxide layer structure is formed of a metal oxide represented by XaYb (X: a metal element, Y: the element oxygen, a: the atomic number of the metal element, and b: the atomic number of the element oxygen), and the atomic percent of the metal element is greater than {a/(a+b)}×100(%). The layer structure is formed of nano-crystalline particles and nano-amorphous particles, and the particles forming the layer structure do not undergo heat-induced growth and heat-induced conversion to crystalline particles.

Abstract: A hydrogen production system that achieves a highly-efficient hydrogen production operation even when a time-varying electric power source is used is provided. A hydrogen production system includes a capacitor inputting electric power energy from a renewable power supply, and storing electric power, a pulse voltage generation unit generating a pulse voltage having a set amplitude and a set cyclic period by using the electric power stored in the capacitor, and an electrolytic cell applying the generated pulse voltage, and generating hydrogen by high temperature steam electrolysis by using steam supplied into the electrolytic cell.

Abstract: Electrochemically reacting a lanthanide or actinide in solvent at a working electrode; wherein the solvent comprises an organic solvent such as acetonitrile which have a dielectric constant of at least three; wherein the solvent system further comprises an electrolyte; wherein the working electrode comprises an ionically conducting or permeable film such as a fluorosulfonate film; wherein at least one ligand such as triflate distinct from the ionically conducting or permeable film is present; wherein the ligand is chemically similar to a structure in the ionically conducting or ionically permeable film; and optionally wherein the electrochemical oxidation or reduction is carried out under the influence of a magnetic field which favorably enhances the reaction. Improved electrochemical methods, identification, and separation can be achieved.