Abstract: A liquid concentrate comprising essentially: (A) 0.1 to 10 wt. % of one or more amphoteric emulsifying agents; (B) 30 to 95 wt. % of one or more nonionic alkoxylated surfactants; (C) 0 to 20 wt. % of one or more glycol-based solubilizers; and (D) 0 to 65 wt. % of one or more organic solvents; wherein component (B) comprises a mixture of C6-C15-alkanol ethoxylates with different carbon numbers for the alkanol unit species, the carbon numbers for the two C6-C15-alkanol ethoxylates which have the highest share in weight in the mixture being at least 1.5 carbon numbers distant from each other, is useful for reducing or eliminating the formation in a liquid hydrocarbon fuel of ice particles having a weight average particle size greater than 1 ?m when said liquid hydrocarbon fuel is cooled to temperatures in the range of from 0 to ?50° C.

Abstract: A process and system for recovering natural gas liquids (NGL) using a combination of J-T cooling and membrane separation. The process involves compressing, separating, and cooling a flare gas stream comprising at least methane and C3+ hydrocarbons prior to being introduced to a J-T valve. The cooled stream exiting the J-T valve is further separated, producing a NGL product stream and an uncondensed gas stream. The uncondensed gas stream is directed to a membrane separation step, which results in a C3+ hydrocarbon enriched stream and a C3+ hydrocarbon depleted stream. The C3+ hydrocarbon enriched stream may be recycled back to the process to recover more NGL.

Abstract: Methods and compositions for treating coal are provided. A nitroxyl radical compound is added to the coal in order to inhibit coal autooxidation. Additionally, the nitroxyl radical compound can be conjointly used with conventional dust control agents, in order to suppress fugitive coal dust dissemination. In those cases in which foamed treatment is desired to treat coal dust, the nitroxyl radical compound can be conjointly used in the foamable compositions, along with a variety of foam building surfactants such as the amphoteric, nonionic and anionic surfactants.

Abstract: Methods of producing clean (e.g., low sulfur and metal ion content, and free of fermentation inhibitors) sugar and lignin-rich streams, and downstream conversion products, from lignocellulosic biomass, may include obtaining non-chemically pretreated, milled lignocellulosic biomass, reacting the milled lignocellulosic biomass with an enzymatic agent to produce a slurry that includes converted monomeric lignocellulosic sugars and lignin-rich residuals, and separating the slurry into a sugar stream that includes the converted monomeric lignocellulosic sugars and a lignin-rich stream that includes the lignin-rich residuals. The sugar stream, not including water, includes at least 75% monomeric lignocellulosic sugar, less than 0.20% sulfur, and less than 3.0% metal ion content, and the lignin-rich stream includes at least 35% lignin and less than 0.50% sulfur.

Abstract: Methods of producing clean (e.g., low sulfur and metal ion content, and free of fermentation inhibitors) sugar and lignin-rich streams, and downstream conversion products, from lignocellulosic biomass, may include obtaining non-chemically pretreated, milled lignocellulosic biomass, reacting the milled lignocellulosic biomass with an enzymatic agent to produce a slurry that includes converted monomeric lignocellulosic sugars and lignin-rich residuals, and separating the slurry into a sugar stream that includes the converted monomeric lignocellulosic sugars and a lignin-rich stream that includes the lignin-rich residuals. The sugar stream, not including water, includes at least 75% monomeric lignocellulosic sugar, less than 0.20% sulfur, and less than 3.0% metal ion content, and the lignin-rich stream includes at least 35% lignin and less than 0.50% sulfur.

Abstract: Gasoline fuel composition suitable for use in an internal combustion engine comprising: (a) Fischer-Tropsch derived naphtha at a level from 2 to 20% v/v; (b) at least one aromatic octane booster present at a level of 0.75 to 8% v/v or less; and (c) a gasoline base fuel; wherein the gasoline fuel composition comprises 40% v/v or less of aromatics. In a preferred embodiment, the Research Octane Number (RON) of the gasoline fuel composition is increased while maintaining the aromatic content of the gasoline fuel composition at a level of 40% v/v or less, based on the gasoline fuel composition.

Abstract: A process for preparing a gasoline octane boosting composition, including contacting a pyrolysis oil with a non-thermal oxygen plasma to produce an oxidized pyrolysis oil; and mixing the oxidized pyrolysis oil with a gasoline additive to produce the gasoline octane boosting composition. The gasoline octane boosting composition can be used in a gasoline blend.

Abstract: A method for reducing timing chain stretch in an engine comprising a step of lubricating said timing chain with a lubricating oil composition that includes a major amount of a base oil; and a minor amount of an additive package including at least one overbased calcium phenate detergent having a total base number of at least 150 mg KOH/g, measured by the method of ASTM D-2896, at least one calcium sulfonate detergent; and at least one magnesium-containing detergent. The lubricating oil composition has a weight ratio of total calcium from the at least one calcium sulfonate detergent to total calcium and magnesium in the lubricating oil composition of from about 0.06 to less than about 0.45.

Abstract: There is provided a flow-resistance reducing laminate comprising: a substrate; and a flow-resistance reducing layer formed on the substrate, wherein the flow-resistance reducing layer has a surface portion facing a liquid, wherein a flow interface is formed between the liquid and the laminate upon relative movement between the liquid and the laminate, wherein the flow-resistance reducing layer is configured such that an air layer defines the flow interface.

Abstract: There are provided a solvent composition containing tDCE, which does not exert an adverse effect on the global environment, has high solubility and incombustibility, and can maintain initial incombustibility even in use accompanied by a phase change, a cleaning method using the solvent composition, a coating film-forming composition including the solvent composition, and a method of forming a homogeneous coating film using the coating film-forming composition. A solvent composition includes: tDCE; a first HFE having a boiling point of 40 to 65° C.; and a second HFE having a boiling point of 70 to 120° C., in which a ratio of tDCE is 65 to 80 mass %, a ratio of the first HFE is 5 to 25 mass %, and a ratio of the second HFE is 5 to 25 mass % with respect to a total amount of tDCE, the first HFE, and the second HFE.

Abstract: (1) A cylinder lubricating oil composition for a crosshead diesel engine, the composition having a sulfated ash content of 2.0 to 5.5 mass %; a base number of 15 to 45 mgKOH/g; and an autoignition temperature of no less than 262° C. (2) A cylinder lubricating oil composition for a crosshead diesel engine, comprising: a lubricant base oil; (B) a Ca sulfonate detergent having a base number of no less than 10 mgKOH/g and less than 60 mgKOH/g; (C) a Ca phenate detergent having a base number of 55 to 200 mgKOH/g; (D?) an amine antioxidant; and (E?) a zinc dithiophosphate, wherein the composition has a base number of no less than 15 mgKOH/g and less than 120 mgKOH/g.

Abstract: The invention is directed to a method for reducing low speed pre-ignition events in a spark-ignited direct injection internal combustion engine by supplying to the sump a lubricant composition which contains an oil of lubricating viscosity and a metal overbased detergent. The metal overbased detergent may be selected from sulfonate detergents, phenate detergents, and salicylate detergents, especially sulfonate detergents with a metal ratio of at least 5.

Abstract: The application relates to compositions comprising compounds of Formula I, Formula II, or Formula III and the use of these compositions in the fragrance industry, i.e., for the production of perfumes, air fresheners, laundry detergents, household cleaning products, liquid or bar soaps, shampoos, conditioners, hair sprays, cosmetics, deodorants, insect repellants, insecticides, and pet litter.

Abstract: The present Invention provides a formulation for use in domestic laundry comprising anionic surfactant and or nonionic surfactant and stearyl ether carboxylic acid.

Abstract: Compositions and methods to improve the cleaning performance of washing agents during laundering of textiles are disclosed. Compositions described use 6-desoxy-6-amino-celluloses. Methods for laundering textiles, in which a detergent and a soil release active ingredient are used in the form of a cellulose derivative as defined above, are also included. These methods can be performed manually or as appropriate with the aid of a conventional domestic washing machine.

Abstract: A gel composition having high adhesion, high whitening resistance, and high high-temperature stability is provided. The gel composition includes a polyoxyalkylene alkyl ether (A) represented by general formula (1) and water (B). R—O—(PO)m-(EO)n—H ??(1) In the formula (1), R is a linear alkyl group having 12 to 22 carbon atoms, PO represents an oxypropylene group, EO represents an oxyethylene group, m and n each represent an average number of moles added, m is a number of 1 to 20, and n is a number of 1 to 80. PO and EO are added in a configuration of block addition.

Abstract: A detergent composition comprising a subtilisin variant having the amino acid sequence set forth in SEQ ID NO: 1, and at least one additional ingredient selected from i) bleaches selected from percarbonates, persulphates and organic peracids, ii) aminocarboxylates, or iii) sulphonated polymers, or iv) organophosphoric acids or salts thereof and mixtures thereof is provided. Also provided is a detergent composition comprising a subtilisin variant having the amino acid sequence set forth in SEQ ID NO: 1, wherein the detergent composition is at least partially enveloped in a water soluble or water dispersible package. The compositions exhibit good performance on proteinaceous stains, even when formulated at alkaline pHs. A method of removing proteinaceous stains from surfaces comprising such stains is also provided.

Abstract: Composition for the removal of biofilms present on a substrate, characterized in that it comprises at least one detergent component comprising a sequestrant and also a wetting agent and a dispersant and at least one enzymatic component containing at least one protease, at least one laccase and at least one polysaccharidase, method of implementation thereof and uses thereof.

Abstract: Provided herein are solid compositions for fabric treatment; unit dose laundry detergent compositions containing solid compositions for fabric treatment; and process of their making and using, e.g., for treating textiles.

Abstract: A method for fabricating an electronic device including a semiconductor memory may include: forming a material layer over a substrate; forming a material pattern by etching the material layer, the etching providing an etch residue on sidewalls of the material pattern; and removing the etch residue, wherein removing of the etch residue includes performing a cleaning process using a cleaning composition including water and a fluorine-containing compound or an amine, and having a pH in a range of 7 to 14.

Abstract: A fermentation basket for suspension in a fermentation tank during wine fermentation which includes a perforated bottom side, at least one perforated vertical side, an open top side having an upper edge with a circumference or perimeter, cable or chain connecting elements disposed around said upper edge for connecting a hoist cable or chain to hoist said filtration basket, and hanging structure disposed around said upper edge for suspending said filtration basket in the interior of a fermentation tank such that said bottom side is spaced apart from the bottom of the fermentation tank.

Abstract: A method of introducing yeast into a fruit juice such as grape must to be fermented is disclosed. The method comprises feeding dry yeast into a flowing stream of the fruit juice and delivering the flowing stream of fruit juice with the yeast in it to a mixer (10). The fruit juice and yeast emerging from the mixer is fed into a fermentation tank. The yeast can include nutrients selected from inactivated yeasts, yeast autolysates, yeast cell hulls, yeast extracts, amino acids, peptides, proteins, sterols, ergosterol, thiamin, biotin, pantothenic acid, niacin, riboflavin, pyridoxine, minerals and inorganic nitrogen in the form of ammonium salts.

Abstract: The present disclosure provides fluids, such as a distilled spirit, comprising at least about 25 ppm of an infused gas. Systems and methods for producing such gas-infused fluids are also provided.

Abstract: An improved system and process for rapidly producing distilled spirits having characteristics associated with a much longer maturation process is provided. The method involves contacting wood with an amount of unmatured distilled spirit under heated conditions and contacting the resulting heat-treated spirit with actinic light. The disclosure provides embodiments where a spirit is sequentially processed through heat and actinic light treatment. The disclosure also provides embodiments where a heat-treated spirit is mixed with a spirit that has been separately treated with light to give characteristics of a mature spirit. Air may also be percolated through a mature spirit in a container with a headspace at a gauge pressure between about ?25 inHg and about ?30 inHg, until the alcohol concentration of the mature spirit is reduced by between about 1% to about 2% by volume, and until the total volume of the mature spirit is reduced by about 10% or less.

Abstract: The present invention is directed to a process for making a concentrated vinegar product by simple evaporation, which concentrated vinegar product can also be used as intermediate in the production of products that can be listed on food labels as “vinegar”.

Abstract: The present invention is directed to a process for making a concentrated vinegar product by simple evaporation, which concentrated vinegar product can also be used as intermediate in the production of products that can be listed on food labels as “vinegar”.

Abstract: An organic waste treatment apparatus according to the present disclosure includes an acid fermenter 140, a methane fermenter 150, a thickener tank 300, and a separation membrane device 400, and includes: a first circulation line 141 in which a first circulation pump 141a is installed in a linked manner so that a part of organic waste being acid-fermented in the acid fermenter 140 is supplied to the methane fermenter 150; and a second circulation line 151 in which a second circulation pump 151a is installed in a linked manner so that a part of a anaerobic digestive fluid being methane-fermented in the methane fermenter 150 is supplied to the acid fermenter 140, in which the thickener tank 300 is installed between the methane fermenter 150 and the separation membrane device 400, supplied with concentrated circulating water from the separation membrane device 400, and supplies at least a part of the supplied concentrated circulating water to the methane fermenter 150 in an indirect injection manner, such that a

Abstract: A device for simulating a function of a tissue includes a first structure, a second structure, and a membrane. The first structure defines a first chamber. The first chamber includes a matrix disposed therein and an opened region. The second structure defines a second chamber. The membrane is located at an interface region between the first chamber and the second chamber. The membrane includes a first side facing toward the first chamber and a second side facing toward the second chamber. The membrane separates the first chamber from the second chamber.

Abstract: A microfabricated device having at least one gas-entrapping feature formed therein in a configuration that entraps air bubbles upon wetting the feature with a solvent or solution is described. The device includes a sacrificial residue in contact with the gas-entrapping feature, the dissolution of which guides the wetting of the gas-entrapping feature.

Abstract: Disclosed is a hollow fiber filter arrangement and a bioreactor system comprising such a hollow fiber filter arrangement. The hollow fiber filter arrangement comprising: an inlet for receiving a sample to be filtered in the hollow fiber filter arrangement; an outlet for delivering of the filtered sample out from the hollow fiber filter arrangement; a bundle of elongate hollow fibers connected at a first end to the inlet and connected at an opposite second end to the outlet; and an outer housing enclosing the bundle, wherein a filtrate/waste collection space is provided between the outer housing and the at least one elongated hollow fiber, and wherein the outer housing is made from a flexible material.

Abstract: Polymeric substrates, optionally in sheet form, treated with a thin layer of photoresist are perforated by laser ablation. Following removal of the photoresist the polymer substrates perforated with holes are patterned in stripes by photolithography, which is followed by synthesis of a cell-adhesive organometallic/self-assembled monolayer of phosphonate (SAMP) interface in the exposed regions, providing well-aligned continuous stripes for various levels of perforation. Cells plated on each of these 2-dimensional (2D) perforated surfaces attach to the interface and spread in alignment with pattern fidelity that is as high as that measured on a non-perforated, patterned substrate. A stack of such 2D patterned polymers yields a 3-dimensional (3D) device which facilitates cell growth and viability via the perforations.

Abstract: According to various implementations, the bioreactor system includes a bioreactor vessel, an impeller for stirring contents within the vessel, a temperature control source for controlling the temperature of the contents of the vessel, an aeration system for supplying air to the vessel, and one or more data loggers. According to certain implementations, the impeller is a novel impeller design that spins more smoothly and rapidly than known impellers. In addition, the bioreactor system is modular, durable, and relatively inexpensive compared to existing bioreactor systems, which allows for bench-scale implementation, use with differently sized bioreactor vessels, and accessibility to more educational programs, according to some implementations.

Abstract: A filter holding device (1; 41; 75; 81) arranged for providing at least one filter (7; 43a, 43b; 85) inside a flexible bag (3; 71), said filter holding device comprising attaching means (19a-d, 21a-d; 79; 83) for attaching the filter holding device to an inner surface of the flexible bag such that the at least one filter held by the filter holding device is provided at a distance from the inner surface of the flexible bag.

Abstract: Methods for introducing exogenous material into a cell are provided, which include exposing the cell to a transient decrease in pressure in the presence of the exogenous material. Also provided are devices for performing the method of the invention.

Abstract: The disclosure provides systems and methods for producing a cannabinoid product, which comprises contacting a cannabinoid precursor in a first phase with a cannabinoid synthase in a second phase, wherein the first phase and the second phase are substantially immiscible or immiscible. The disclosure also provides a composition comprising the cannabinoid precursor in a first phase and a cannabinoid synthase in a second phase, wherein the first phase and the second phase are substantially immiscible or immiscible.

Abstract: A low-oxygen system is directed to culturing a diverse microbiome and includes an anaerobic chamber and at least one microfluidic device removably inserted in the anaerobic chamber. The microfluidic device includes a first microchannel in which a first level of oxygen is maintained, and a second microchannel in which a second level of oxygen is maintained, the second level of oxygen having a greater oxygen concentration than the first level of oxygen. The microfluidic device further includes a membrane located at an interface region between the first microchannel and the second microchannel, the membrane further having a plurality of pores via which oxygen flows from the second microchannel to the first microchannel to form an oxygen gradient in the first microchannel. The system further includes a culture system provided in the first microchannel and including oxygen-sensitive anaerobic bacteria.

Abstract: Aspects relate to automated cell culture incubators comprising imaging systems, e.g., holographic or phase-contrast imagers, configured for imaging said cells within the incubators.

Abstract: A cell culture monitoring system includes measuring units housed in an incubator and each equipped with an indicator, the measuring units each measuring a state of cells in a culture container mounted therein; and an administration unit. The administration unit includes a receiver that receives, from each of the measuring units, a state of the cells measured, the state being associated with identification information of the measuring unit; an analyzer that analyzes the state of the cells and computes information indicating a culture state; a storage that stores, in association with each other, the information indicating the culture state and the identification information; a display that displays, in association with each other, the information indicating the culture state and an identification based on the identification information; and a controller that controls the indicators so that each of the indicators displays the identification corresponding to the identification displayed on the display.

Abstract: The invention relates to biotechnology. The plankton strain of the unicellular green alga Chlorella vulgaris GKO, which has a thin cell wall, deposited in the Russian National Collection of Industrial Microorganisms under the registration number of VKPM A1-24. The strain VKPM A1-24 of the unicellular green alga Chlorella vulgaris can be used to produce food biomass intended for preparation of a beverage, concentrate, paste or dry powder. The invention makes it possible to shorten the period of cultivation of the biomass of unicellular algae.

Abstract: Methods and compositions herein provide non-naturally occurring ?-butyrolactones (GBLs) in racemic mixtures that increase efficiency and effectiveness of screening for production of antibiotics, and enhance yields and express silent pathways. Non-naturally occurring GBLs were synthesized and found to stimulate antibiotic production in several different streptomycete strains. Antibiotic production by Streptomyces coelicolor was induced by a racemic mixture of non-cognate stereoisomers of VB-D, seven of which are non-naturally occurring. Further, novel A-factor-type GBL analogs stimulated antibiotic production in S. coelicolor. Synthesis in response to the treatment with the non-cognates GBL was observed for known compounds including undecylprodigiosin, desferrioxamine and streptorubin B, as was synthesis of a compound of unknown structure.

Abstract: Compositions and methods are described for preparing media, feeds, and supplements. Such methods and medias may display increased stability of labile components and may use, for example, microsuspension and/or encapsulation technologies, chelation, and optionally, coating and/or mixing the labile compounds with anti-oxidants. The compositions may withstand thermal and/or irradiation treatment and have reduced virus number. These techniques may result in product with extended shelf-life, extended release of their internal components into culture, or in product that can be added aseptically into a bioreactor using minimal volumes. The compositions and methods may optimize the bioproduction workflow and increase efficiency.

Abstract: A micro-scale artificial gland is disclosed in the form of an independent unit for promoting biological activity. The artificial gland includes cells formed in a membrane enclosing a reservoir. The reservoir is a bio-reactor capable of containing a product of activity of the cells. The reservoir comprises a gas, a liquid, and a gel and preferably also contains nanoparticles, a buffer, a surfactant, and, a gel precursor. The reservoir may also contain cells. Nanoparticles may also surround the artificial gland to form a protective coating. A variety of methods are disclosed for making the artificial gland by directed assembly of cells into the artificial micro-gland by gel, liquid or bubble templating. All involve coating the surface of gel, droplet or bubble with the living cells and the stabilizing the cells on the surface of gels, droplets or bubbles.

Abstract: The invention provides for methods of obtaining cells from mammalian, preferably human, postpartum umbilical cord arterial tissue and use of these cells to treat ocular degenerative conditions. In particular, methods of the invention result in the isolation of a homogenous population of human postpartum umbilical cord arterial tissue-derived cells. In certain embodiments, the cells: express CD13, CD90, NG2, and HLA-ABC; do not express one or more of CD31, CD34, CD45, CD117, FSP1, and E-cadherin. In other embodiments, the cells secrete one or more of thrombospondin-1, BDNF and sVEGFR1.

Abstract: The presently disclosed subject matter provides for in vitro methods of inducing differentiation of human stem cells into cortical neurons, and cortical neurons generated by such methods. The presently disclosed subject matter also provides for uses of such cortical neurons for treating neurodegenerative CNS disorders.

Abstract: Embodiments described herein relate to compositions including genetically modified CAR cells and uses thereof for treating cancer. Some embodiments of the present disclosure relate to compositions and methods for T cell response enhancement and/or CAR cell preparation. For example, a method may include obtaining cells comprising a CAR and culturing the cells in the presence of an agent that is recognized by the extracellular domain of the CAR.

Abstract: Provided herein are methods of producing natural killer (NK) cells and/or ILC3 cells using a three-stage expansion and differentiation method with media comprising stem cell mobilizing factors. Also provided herein are methods of suppressing tumor cell proliferation using the NK cells and/or ILC3 cells and the NK cell and/or ILC3 cell populations produced by the three-stage methods described herein, as well as methods of treating individuals having cancer or a viral infection, comprising administering the NK cells and/or ILC3 cells and the NK cell and/or ILC3 cell populations produced by the three-stage methods described herein to an individual having the cancer or viral infection.

Abstract: The present invention relates to novel methods of growing at least 100 million minimally-passaged fibroblasts from a small biopsy specimen. The invention includes methods wherein a small biopsy specimen is seeded directly into a large tissue culture flask, and passaged no more than three times.

Abstract: Methods and compositions for producing cells expressing CD56/Pax3, CD56Pax7, and/or Pax3/Pax7 are provided herein. In some instances, the method involves contacting a pluripotent stem cell in an in vitro culture with one compound, or with two or more compounds at the same time, wherein the contacting the pluripotent stem cell in the in vitro culture with one compound, or with two or more compounds at the same time, directly results in generation of cells expressing CD56/Pax3, CD56/Pax7, or Pax3/Pax7. Also provided are methods of using the generated cells. Typically one of the compounds is a wnt pathway activator, such as the GSK3 inhibitor CHIR99021.

Abstract: It is an object of the present invention to provide a method for producing frozen mesenchymal cells maintaining a viability and an adhesion rate similar to those of cells obtained through a step of culture, without undergoing a step of culturing cells before cryopreservation step. The method comprises dissociating cells by treating a tissue with proteolytic enzymes and cryopreserving the cells. The present invention provides a method for producing frozen mesenchymal cells maintaining a viability and an adhesion rate similar to those of culturing cells without a step of culturings cells before cryopreserving.

Abstract: The present invention provides a basal cell culture medium and a feed medium with novel amino acid ratios and/or iron choline citrate as iron carrier that result in improved performance of mammalian cell culture processes, such as CHO cultivation and protein production processes, in particular in increased product titer (e.g. of monoclonal antibodies). Also provided are methods for culturing mammalian cells and producing a protein of interest using said basal cell culture medium and optionally feed medium. The invention also provides for a medium platform that comprises (i) the basal cell culture medium and (ii) the feed medium.