Abstract: Disclosed are a device and a method for manufacturing a natural gas hydrate. Provided is the device for manufacturing a natural gas hydrate comprising: an ice slurry generation unit for preparing ice slurry having 13-20% of ice at normal pressure; a first pipe, having one end connected to the ice slurry generation unit for withdrawing the ice slurry from the ice slurry generation unit, and in which a high-pressure pump for increasing pressure on the ice slurry is interposed; a hydrate preparation reactor, which is connected to the other end of the first pipe so as to receive the pressurized ice slurry, and to which natural gas is supplied and mixed, for generating natural gas hydrate slurry; a second pipe, having one end connected to the hydrate preparation reactor, for withdrawing the natural gas hydrate slurry; and a dehydrating portion, which is connected to the other end of the second pipe, for dehydrating the natural gas hydrate slurry.

Abstract: A dispersant includes the reaction product of an amine and at least one equivalent of glycidyl ether where the amine is selected from a group consisting of aminoethylpiperazine, bis(2-(piperazin-1-yl)ethyl)amine, 4,4?-methylenebiscyclohexylamine, m-xylenediamine, diethylenetriamine, and triethylenetetramine and where the glycidyl ether has the structure (A), where R is selected from aromatic carbon chains, non-aromatic carbon chains and polyalkylene glycol groups. The dispersant is useful in a lubricant with a base oil for increasing soot dispersibility of the lubricant.

Abstract: Lubricant compositions including an additive composition and methods for its use in engines that produce soot. The lubricant composition contains a base oil and an additive composition having (a) at least 0.05 percent by weight of a first dispersant that is a reaction product of A) a hydrocarbyl-dicarboxylic acid or anhydride, and B) at least one polyamine; and (b) at least 0.05 percent by weight, both based on a total weight of the lubricant composition, of a second dispersant that is a reaction product of A?) a hydrocarbyl-dicarboxylic acid or anhydride, and B?) at least one polyamine, wherein the reaction product is post-treated with C) an aromatic carboxylic acid, an aromatic polycarboxylic acid, or an aromatic anhydride wherein all carboxylic acid or anhydride groups are attached directly to an aromatic ring, and/or D) a non-aromatic dicarboxylic acid or anhydride having a number average molecular weight of less than about 500.

Abstract: The present invention relates to novel compounds and their use as fragrance materials.

Abstract: Lipid-based wax compositions and their methods of making are provided for compositions substantially free of fat bloom. The compositions comprise 0.1-10 percent by weight triacylglycerides, 30-95 percent by weight monoacylglycerides and diacylglycerides combined, and 0.1-65 percent by weight fatty acids. The methods comprise blending the monoacylglycerides, diacylglycerides, triacylglycerides, and fatty acids by heating the lipid-based wax composition at a sufficiently high temperature to destroy substantially all crystal structure within the lipid-based wax composition. The methods further comprise pouring the lipid-based wax composition into a mold or a container having a surface and a core, wherein the pouring is conducted at a temperature at least 15° C. greater than the congeal point of the lipid-based wax composition. The methods further comprise cooling the lipid-based wax composition under conditions sufficient to cool the core to at least 5° C.

Abstract: The invention discloses detergent compositions containing acrylic acid polymers, including methacrylic acid/ethyl acrylate polymers. In certain embodiments compositions employ an acrylic acid polymer comprising at least 40 wt-% polymerized residues of acrylic monomers, at least 50 wt-% of at least one surfactant, a solvent, at least one water conditioning polymer, and water. The detergent compositions provide increased soil removal and soil suspension when treating textiles, namely through use of industrial laundering machinery. The detergent compositions and methods of employing the same beneficially clean and prevent redeposition of soils containing high concentrations of oil and metal particulates, as are customary in industrial laundering soils.

Abstract: An aqueous cleaning composition, which may be suitable for use in cleaning hard surfaces, is described. The aqueous cleaning composition includes an ionic liquid solvent, an amino alcohol, and a substantial amount of water. The cleaning compositions may also include a disinfecting quaternary surfactant, a nonionic surfactant, such as an ethoxylated alcohol and/or alkyl polyglycoside, and/or a chelating agent, such as an aminopolycarboxylate chelating agent.

Abstract: The present invention relates to the use of branched polyesters obtainable by polycondensation of citric acid with at least one polyalcohol, and optionally with polycarboxylic acid component as additive in washing compositions, cleaners, detergents or a formulation for water treatment and to mixtures comprising such branched polyesters. The invention further relates to the use of hydrophobically modified branched polyesters, and to the method for cleaning, washing or water treatment using such branched polyesters.

Abstract: Stabilized use solutions of low phosphorus, alkali metal carbonate detergents employing enzymes for cleaning compositions are disclosed. In particular, the present invention is a composition for, and method of, removing soils, preventing redeposition of protein soils and reducing foam, using stabilized enzyme cleaning compositions, namely use solutions of the same.

Abstract: A cleaning method and a cleaning fluid are provided. The cleaning method includes accessing a plurality of turbine components attached to a turbine assembly, the turbine assembly being a portion of a turbomachine, positioning at least one cleaning vessel over at least one of the turbine components, forming a liquid seal with a sealing bladder, providing a cleaning fluid to the cleaning vessel, and draining the cleaning fluid from the cleaning vessel. The cleaning fluid includes a carrier fluid and a solvent additive for removing fouling material from the turbine component. An alternative cleaning method is also provided.

Abstract: A beer filter and a method of brewing beer is disclosed, the beer filter including an outer sheath, an outer permeable sheath disposed in the outer sheath, and an inner permeable sheath disposed in the outer permeable sheath. The method includes steps of disposing an outer permeable sheath within an outer sheath, filling an outer permeable sheath with hops, disposing an inner permeable sheath within the outer permeable sheath, and causing beer to flow from a vessel through and out of the inner permeable sheath, through the hops in the outer permeable sheath, out of the outer permeable sheath and into the outer sheath, and from the outer sheath and back into the vessel.

Abstract: A process and apparatus for converting sequestered carbon to fuel, such as methane, and/or materials, such as fermentation substrates, biopolymers, bioplastics, oils, pigments, fibers, proteins, vitamins, fertilizers and animal feed. The apparatus comprises a deep well carbon-sequestering bioreactor coaxially located within a deep well anaerobic bioreactor. Carbon is sequestered into a photosynthetic biomass or a heterotrophic biomass, which is subsequently digested by an anaerobic biomass containing methanogenic microbes, whereby methane is a digestion product.

Abstract: Methods and systems are provided for a stem cell organ device including an array of alternating stem cell channels and fluid channels. In one example, a method may include loading a stem cell channel with stem cells and flowing blood through a fluid channel in order to allow an exchange of molecules between the stem cells and the blood.

Abstract: A number of novel improved microfluidic configurations and systems and methods of manufacture and operation for a microfluidic invasion assay system.

Abstract: The present invention relates to large-scale bioreactors having at least two impellers, large-scale bioreactor systems and methods for the large scale cultivation and propagation of mammalian cells using these bioreactors.

Abstract: Methods and systems for magnetic mixing. Particular embodiments relate to applying a magnetic field to move a magnetically responsive component in a chamber.

Abstract: A stable and scalable process is provided to manufacture reduced serum umbilical cord tissue-derived (UTC)-conditioned media. The method includes the culture of a UTC under reduced serum conditions. Subsequently, the UTC is washed and grown in serum-free basal media. After approximately 24 hours, the conditioned media is collected, filtered and concentrated by use of an approximately 5 kDa or similar cut-off membrane.

Abstract: The invention provides methods and compositions relating to differentiated cell populations that derive from pluripotent stem cells. The methods relate to reducing the number of residual stem cells present in such populations. The compositions include differentiated cell populations that contain reduced number of stem cells or that contain no stem cells. Pluripotent stem cells may be reduced in number and/or function through exposure to low oxygen levels.

Abstract: This disclosure relates to compositions comprising human preprimitive streak cells and/or human mesendoderm cells as well as methods for their production. Additionally, disclosed herein are methods of identifying factors useful in the further differentiation of preprimitive streak and mesendoderm cell types.

Abstract: The present invention relates to a microorganism having improved L-lysine-producing ability and an L-lysine-producing method using the same. More specifically, the present invention relates to a microorganism of the genus of Corynebacterium, in which acetate kinase activity is further enhanced over inherent activity, and an L-lysine-producing method using the same.

Abstract: The present invention relates to isolated polypeptides having cellobiohydrolase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

Abstract: The present invention relates to the conjugation of Factor VII polypeptides with heparosan polymers. The resultant conjugates may be used to deliver Factor VII, for example in the treatment or prevention of bleeding disorders.

Abstract: Provided are compositions comprising newly identified protein fragments of aminoacyl-tRNA synthetases, polynucleotides that encode them and complements thereof, related agents, and methods of use thereof in diagnostic, drug discovery, research, and therapeutic applications.

Abstract: The present disclosure is directed to genetically engineered bacteria strains with enhanced biofuel-producing capabilities from cellulosic substrates. The bacteria strains of the present disclosure comprise an inactivated Type I glutamine synthetase gene. The present disclosure is also directed to methods of producing biofuels from cellulosic biomass using the genetically engineered bacteria strains.

Abstract: Provided are compositions comprising newly identified protein fragments of aminoacyl-tRNA synthetases, polynucleotides that encode them and complements thereof, related agents, and methods of use thereof in diagnostic, drug discovery, research, and therapeutic applications.

Abstract: The invention provides methods and compositions for a mutein aminoacyl-tRNA synthetase that preferentially charges a tRNA with a non-natural amino acid. Also provided are methods for incorporating the non-natural amino acids, pyridinyl-amino tetrazine amino acids, into a protein, and further conjugating a biologically active adduct to the pyridinyl-amino tetrazine.

Abstract: A rack for holding samples and various reagents, wherein the rack may be used for loading the samples and reagents prior to using the reagents. The rack accepts complementary reagent holders, each of which contain a set of reagents for carrying out a predetermined processing operation, such as preparing biological samples for amplifying and detecting polynucleotides extracted from the samples.

Abstract: Strategies, systems, methods, reagents, and kits for phage-assisted continuous evolution are provided herein. These include strategies, systems, methods, reagents, and kits allowing for stringency modulation to evolve weakly active or inactive biomolecule variants, negative selection of undesired properties, and/or positive selection of desired properties.

Abstract: The current invention provides an improved oligonucleotide and its use for treating, ameliorating, preventing and/or delaying DMD or BMD.

Abstract: The present invention is directed to anti-sense microRNA-328 in a form of oligodeoxyribonucleotides, or locked nucleic acid (LNA)-modified, and phosphorothioated (PS) bond-modified oligonucleotides. The present invention is also directed to a pharmaceutical composition comprising the anti-sense microRNA-328 composition and a pharmaceutically acceptable carrier. The present invention is further directed to a method for preventing or treating myopia by administering to a subject the anti-sense microRNA-328 composition. A preferred route of administration is topical administration to the eyes.

Abstract: The present invention concerns a CDC25A phosphatase inhibitor for use in the treatment of a drug resistant cancer and/or in prevention of tumor relapse in a patient suffering or having suffered from cancer.

Abstract: The present invention provides a cocaine aptamer represented by the following chemical formula (CI), R-DNA-L-Fc??(CI) where R represents R1-PO4—(CH2)n1—; R1 is selected from the group consisting of a hydrocarbon group and the derivative thereof; n1 represents a natural number; DNA consists of a gene sequence capable of binding to cocaine; L is a linker represented by -L1-(CH2)n2-L2-; L1 is absent or an optional linker; L2 is absent or an optional linker; n2 represents a natural number; and Fc represents a ferrocene group. The present invention provides a cocaine aptamer capable of detecting cocaine with high sensitivity.

Abstract: Transgenic plants are provided comprising a plurality of transgenes comprised in a single locus. In certain aspects, 7 or more transgenes may be expressed from a first locus. Methods are provided for transformation of plant cells with a plurality of transgenes. Also provided are methods for expressing and enhancing the expression of one or more transgenes in a plant.

Abstract: The present invention provides an isolated nucleic acid molecule comprising, or consisting of, the nucleic acid sequence of SEQ ID NO:1 or a nucleic acid sequence of at least 400 bp having at least 80% identity to said sequence of SEQ ID NO:1, wherein said isolated nucleic acid molecule specifically leads to the expression in Muller cells of a gene when operatively linked to a nucleic acid sequence coding for said gene.

Abstract: Methods and compositions for increasing transgene expression and/or activity, including for increasing nuclease-mediated genomic modifications.

Abstract: The present invention provides novel multigenome retroviral vectors, methods and packaging systems for making such retroviral vectors and methods of use.

Abstract: Engineered microorganisms are provided that convert gaseous substrates, such as producer gas, into limonene. In some embodiments, limonene is pumped out of the cell via an efflux pump. In some embodiments, limonene, produced as described herein, is converted through catalytic dimerization into jet fuel. Producer gas used in the processes described herein for production of limonene may be derived from sources that include gasification of waste feedstock and/or biomass residue, waste gas from industrial processes, or natural gas, biogas, or landfill gas.

Abstract: The present invention relates to a method for producing polyphenol compounds, i.e. phloroglucinol or one of its derivatives, with a polyketide synthase of type III (PKSIII) from a brown marine alga. The invention also relates to recombinant nucleic acids coding for a polyketide synthase of type III (PKSIII) from the brown alga Ectocarpus siliculosus (E. siliculosus), to recombinant vectors comprising these nucleic acids, as well as to host cells comprising these vectors. Finally, the invention relates to a method for preparing of various compounds by means of polyphenol compounds produced according to the aforementioned method.

Abstract: The present invention provides methods of processing a biomass, e.g., for producing lipid and non-lipid materials, by inducing autolysis of a biomass. The biomass may be generated using any species of microorganism and any available biodegradable substrate, including waste materials. The lipid and non-lipid materials are present in two separate layers of the autolysate, and they can be used to generate valuable products such as biofuels and nutritional supplements. The present invention further provides a processing apparatus useful for practicing the methods of the present invention.

Abstract: The present invention relates to a novel process for cyclizing geranyllinalool using the squalene-hopene cyclase from Zymomonas mobilis (Zm-SHC) or a cyclase with at least 80% sequence identity to the Zm-SHC, and cyclization products obtained in this process.

Abstract: A method of producing a sugar liquid includes hydrolyzing with a filamentous fungus-derived cellulase a product obtained by adding a water-soluble inorganic salt(s) to a pretreated product of cellulose to a final concentration of 5 to 35 g/L; and subjecting the hydrolysate to solid-liquid separation and filtering the obtained solution component through an ultrafiltration membrane to recover the filamentous fungus-derived cellulase as a non-permeate and to obtain a sugar liquid as a permeate.

Abstract: The present invention relates to methods and compositions for preventing incorporation of norleucine into proteins during recombinant protein production in bacteria. The present invention also provides microorganism host cells and nucleic acid molecules for use with the methods and compositions provided herein.

Abstract: Devices, methods, and kits for easy, accurate, and fast estimation of soil microbial load are provided. The devices include membranes that are rapidly wettable with aqueous fluids and retain soil microorganisms of up to 200 micrometers in size, without retaining soil pigments. The method requires reconstituting a soil sample in an extraction fluid so that the microorganisms in the soil sample are released from particles of the soil sample and into the extraction fluid. The method does not require a filtration step. The extraction fluid containing the microorganisms suspended therein is then pipetted onto the membrane device, or the membrane is dipped into the extraction fluid. The color appearing on the membrane is then compared to color intensity gray scale strips to determine the soil microbial load.

Abstract: The present invention provides a method for determining the presence of a microorganism in a sample using an electrochemically active reporter, wherein the method comprises (a) contacting the sample with an electrochemically active reporter, wherein the electrochemically active reporter is a conjugate comprising a sugar moiety and a redox active reporter moiety that are covalently linked such that the covalent bond can be enzymatically cleaved in the presence of the microorganism by an enzyme expressed by the microorganism, wherein the redox active reporter moiety is selected from the group consisting of resorufin and compounds of formula (I) as defined herein, under conditions that allow enzymatic cleavage of the covalent bond between the sugar moiety and the redox active reporter moiety and reduction of the redox active report moiety in the presence of the microorganism; (b) electrochemically determining the released redox active reporter moiety; and (c) determining the presence of the microorganism and, op

Abstract: In a first aspect, the present invention relates to a method for screening bacteria on their susceptibility against candidate compounds. In a further aspect, the present invention relates to a method for screening the antibiotic efficacy of candidate compounds suppose to have an antibiotic activity on bacteria. Moreover, the present invention relates to a method for forming a bacterial biofilm on a support, a system allowing in vitro and in vivo evaluation of biofilms formed by bacteria as well as methods for the stratification of the treatment regimen against bacterial infections.

Abstract: The invention provides probes with formula (I): in which: X1?NH, O, or S; X2?O or S; SE is a labile group, which may be eliminated under the action of a stimulus that may in particular be the presence of an enzyme, a chemical compound, or a physicochemical characteristic of the medium in which the probe is located; A is an aromatic group that, following cleavage of the C(X2)—O bond in aqueous solution, leads to the liberation of a chromophore or a fluorophore, R represents a hydrogen atom or -(L)n-GP, in which n is equal to 0 or 1, L is a linker arm, and GP is a hydrosolubilizing group; as well as their physiologically acceptable salts, solvates, or hydrates. The invention also provides intermediates for the synthesis thereof and detection methods employing them.

Abstract: The present application relates to detection units and methods for detecting one or more target analytes in a sample using a complex formed by a target and first and second probes, wherein the complex comprises an elongated region, a particle that is coupled to the first probe, and a solid support that is coupled to the second probe. Specific binding of a target analyte can be distinguished from non-specific binding of the particle by measuring the displacement of the particle.

Abstract: The present invention provides methods for immobilizing target nucleic acids on a solid support utilizing combinatorial capture probe pairs. These pairs contain first and second capture oligonucleotides that each comprise a target binding region, a capture region and a stem region positioned between the target binding and capture regions. The target binding regions comprise nucleic acid sequences that allow them to hybridize to adjacent regions on the target nucleic acid. The stem regions have nucleic acid sequences that are complementary to each other and the capture regions each comprise a sequence that when positioned adjacent to one another produce a combined nucleic acid sequence that is complementary to a portion of an oligonucleotide bound to a solid support.

Abstract: Methods of labelling one or more subcellular components (e.g., an organelle and/or subcellular region) in vivo are provided. Methods of labelling a protein in vivo are provided. Methods of determining a nucleic acid sequence in situ are also provided.

Abstract: The invention relates to a hybrid structure including perforated solid substrate having at least one nanopore perforating therethrough, and devices and uses thereof.