Patents Issued in February 26, 2019
  • Patent number: 10214744
    Abstract: The present invention relates to an RNAi-inducing nucleic acid molecule having a new structure and the use thereof, and more particularly to a novel nucleic acid molecule having a structure comprising a first strand, which is 24-121 nt in length and comprises a region complementary to a target nucleic acid, and a second strand which is 13-21 nt in length and has a region that binds complementarily to the region of the first strand, which is complementary to the target nucleic acid, so that the nucleic acid molecule inhibits the expression of a target gene with increased efficiency, and to a method of inhibiting the expression of a target gene using the nucleic acid molecule. The nucleic acid molecule structure of the present invention increases the efficiency with which the nucleic acid molecule inhibits the target gene.
    Type: Grant
    Filed: March 30, 2017
    Date of Patent: February 26, 2019
    Assignee: Sungkyunkwan University Foundation For Corporate Collaboration
    Inventor: Dong Ki Lee
  • Patent number: 10214745
    Abstract: The present invention relates to antisense oligonucleotides that modulate the expression of and/or function of Brain derived neurotrophic factor (BDNF), in particular, by targeting natural antisense polynucleotides of Brain derived neurotrophic factor (BDNF). The invention also relates to the identification of these antisense oligonucleotides and their use in treating diseases and disorders associated with the expression of BDNF.
    Type: Grant
    Filed: January 4, 2017
    Date of Patent: February 26, 2019
    Assignees: The Scripps Research Institute, Curna, Inc.
    Inventors: Mohammad Ali Faghihi, Carlos Coito
  • Patent number: 10214746
    Abstract: The present disclosure relates in part to a method for identifying a soybean germline transformant from a population of soybean transformants by incorporating a selection agent within rooting medium used in tissue culture during the soybean transformation process. The soybean germline transformants are selected from a population of soybean transformants which are comprised of a combination of non-germline and germline soybean transformants. The soybean non-germline transformants are identified and eliminated early in the transformation process. The soybean germline transformants are identified and selected for culturing into mature soybean plants. The method is readily applicable for screening and obtaining a soybean germline transformant at an early stage in the soybean transformation process.
    Type: Grant
    Filed: October 2, 2014
    Date of Patent: February 26, 2019
    Assignee: Dow AgroSciences LLC
    Inventors: Sivarama Reddy Chennareddy, Dayakar Pareddy, Jayakumar Pon Samuel, Rodrigo Sarria-Milan, Toby Cicak
  • Patent number: 10214747
    Abstract: A new platform method to purify plant-based monoclonal antibodies is provided. Such a method includes an antibody purification platform that involves a standardized procedure for the production of a wide array of different antibodies within a simplified context. The versatility of the overall purification process accords a one-size-fits-all approach for myriad antibody products and includes plant tissue harvesting, extraction and clarification, filtrate generation, a succession of column chromatography procedures, and buffer exposure to provide the desired monoclonal antibodies in proper filtered and purified form for further incorporation and/or use within medicaments and other formulations. Thus, the purified monoclonal antibodies produced thereby such a method are also encompassed within this invention.
    Type: Grant
    Filed: October 3, 2014
    Date of Patent: February 26, 2019
    Assignee: KENTUCKY BIOPROCESSING, INC.
    Inventors: Josh Morton, Barry Bratcher, Kelsi Swope, Emmett Ernest Hiatt, III, Steven D. Hume, Larry Zeitlin
  • Patent number: 10214748
    Abstract: Provided are isolated polypeptides which are at least 80% homologous to SEQ ID NO: 474-643, 645-679, 681-755, 757-760, 4806-6390, 6395-6396, 6401-6895, 6897-7249, 7251-7685, 7687-7693, 7695-7700, 7702-7708, 7710-7796, 7798-7816, 7818, 7820-7837, 7839-7840, 7842-7861, 7863-8134, 8136-8163 or 8164, isolated polynucleotides which are at least 80% identical to SEQ ID NOs: 1-170, 172-267, 269-424, 426-473, 761-2486, 2489-2494, 2496-4803 or 4804, nucleic acid constructs comprising same, transgenic cells expressing same, transgenic plants expressing same and method of using same for increasing yield, harvest index, abiotic stress tolerance, growth rate, biomass, vigor, oil content, photosynthetic capacity, seed yield, fiber yield, fiber quality, fiber length, and/or nitrogen use efficiency of a plant.
    Type: Grant
    Filed: January 9, 2018
    Date of Patent: February 26, 2019
    Assignee: Evogene Ltd.
    Inventors: Ilya Pankratov, Hagai Karchi
  • Patent number: 10214749
    Abstract: Isolated polynucleotides having a nucleic acid sequence at least 80% homologous to SEQ ID NO:1, 3, 5, 7, 9, 11, 158, 159, 160, 161, 162-204, 206-211, 214-287 and/or encoding polypeptides having an amino acid sequence at least 80% homologous to SEQ ID NO: 2, 4, 6, 8, 10, 12, 13-56, 58-63, 66-121, 141-156 or 157 are provided. Also provided are methods of utilizing same for increasing the tolerance of a plant to abiotic stresses and/or increasing the biomass, vigor and/or yield of a plant.
    Type: Grant
    Filed: September 5, 2016
    Date of Patent: February 26, 2019
    Assignee: Evogene Ltd.
    Inventors: Hagai Karchi, Gil Ronen, Rodrigo Yelin, Larisa Rabinovich
  • Patent number: 10214750
    Abstract: Systems and methods are disclosed herein for use in transducing, activating, and otherwise treating cells. Cells are introduced into an inner layer of a multi-layered stack that defines at least one flow chamber and a plurality of cell entrainment regions. Vertical flow through the stack entrains the cells in the cell entrainment regions along with genetic information introduction agents or other additives, before the cells are washed using a reverse vertical flow and are collected from the device.
    Type: Grant
    Filed: June 5, 2017
    Date of Patent: February 26, 2019
    Assignee: The Charles Stark Draper Laboratory, Inc.
    Inventors: Jeffrey T. Borenstein, Joseph L. Charest, Christopher M. DiBiasio, Dorit Berlin, Jenna Balestrini, Jose A. Santos, Vishal Tandon
  • Patent number: 10214751
    Abstract: The present invention provides a method for treatment of biomass material by fermentation, said method comprises a pre-treatment of the biomass material by thermal hydrolysis and wet explosion, resulting in an intermediate product having a dry matter concentration above 25% and temperature above 90° C. which is to be introduced to the fermentation, wherein a part of the content of the digestion tank used for the fermentation is recirculated and mixed with a part of the intermediate product from the pre-treatment.
    Type: Grant
    Filed: October 29, 2015
    Date of Patent: February 26, 2019
    Assignee: CAMBI TECHNOLOGY AS
    Inventors: Paal Jahre Nilsen, Hans Rasmus Holte
  • Patent number: 10214752
    Abstract: This document describes biochemical pathways for producing 1,3-butanediol using a polypetide having ?-ketothiolase activity to form a 3-oxo-5-hydroxypentanoyl-CoA intermediate that can be enzymatically converted to 1,3-butanediol, as well as recombinant hosts producing 1,3-butanediol.
    Type: Grant
    Filed: November 13, 2015
    Date of Patent: February 26, 2019
    Assignee: INVISTA NORTH AMERICA S.A.R.L.
    Inventor: Pawel Mordaka
  • Patent number: 10214753
    Abstract: The present invention provides methods for producing cannabinoids and cannabinoid analogs as well as a system for producing these compounds. The inventive method is directed to contacting a compound according to Formula I or Formula II with a cannabinoid synthase. Also described is a system for producing cannabinoids and cannabinoid analogs by contacting a THCA synthase with a cannabinoid precursor and modifying at least one property of the reaction mixture to influence the quantity formed of a first cannabinoid relative to the quantity formed of a second cannabinoid.
    Type: Grant
    Filed: May 12, 2017
    Date of Patent: February 26, 2019
    Assignee: TEEWINOT TECHNOLOGIES LIMITED
    Inventors: Richard Peet, Malcolm J. Kavarana, Robert Winnicki, Marc Donsky, Mingyang Sun
  • Patent number: 10214754
    Abstract: Provided is a transformant which can produce lactic acid with a high productivity without requiring neutralization with an alkali and is excellent in both of lactic acid production capability and growth ability and its production process, and a method for producing lactic acid by using the transformant. A transformant comprising 3 copies of a human lactate dehydrogenase gene that is introduced into a Schizosaccharomyces pombe host, wherein a gene encoding pyruvate decarboxylase 2 of the Schizosaccharomyces pombe host is deleted or inactivated.
    Type: Grant
    Filed: October 6, 2015
    Date of Patent: February 26, 2019
    Assignee: JMTC Enzyme Corporation
    Inventors: Futoshi Hara, Ayako Kashima, Shuichiro Kimura
  • Patent number: 10214755
    Abstract: A method to prepare functional polyester polyols by using micro-reaction device, wherein mixing ?-caprolactone/?-valerolactone monomer with mercapto alcohol evenly with appropriate organic solution under moistureless conditions, and continuously transferring the prepared mixing solution into a micro-reaction device supported with an immobilized enzyme for polymerization to synthetize a poly (?-caprolactone/?-valerolactone). Compared with the prior art, the present invention achieves a continuous production by using immobilized lipase Novozyme435 as a catalyst.
    Type: Grant
    Filed: November 16, 2016
    Date of Patent: February 26, 2019
    Assignee: NANJING TECH UNIVERSITY
    Inventors: Kai Guo, Weijun Huang, Ning Zhu, Xin Hu, Zheng Fang, Yihuan Liu
  • Patent number: 10214756
    Abstract: There is provided a method for generating an oil/fat component by means of culturing algae, in which marine algae belonging to chlamydomonas are cultured in a culture medium containing sea salt.
    Type: Grant
    Filed: March 17, 2014
    Date of Patent: February 26, 2019
    Assignees: National University Corporation KOBE University, Inter-University Research Institute Corporation National Institutes of Natural Sciences, DIC Corporation
    Inventors: Akihiko Kondo, Tomohisa Hasunuma, Shih-hsin Ho, Jun Minagawa, Haruo Nishie, Hiroyuki Taroda, Jo-Shu Chang
  • Patent number: 10214757
    Abstract: A photosynthetic device includes a main body defining a microfluid chamber for causing photosynthesis therein, and a light source, wherein the microfluid chamber is constituted by at least one communication room, a plurality of micro channels respectively and spatially communicated with the communication room, at least one micro injection duct spatially communicated with the communication room, and a plurality of filter plugs spatially connected to the micro channels respectively and the micro injection duct at free ends thereof in order to filter fluid backflow in the micro channels and the micro injection duct. The light source radiates ceaselessly one of the communication room, the plurality of micro channels and the micro injection duct. The photosynthesis is resulted once chloroplasts and normal saline solution are injected into one of the communication room, the plurality of micro channels and the micro injection duct.
    Type: Grant
    Filed: April 10, 2017
    Date of Patent: February 26, 2019
    Inventor: Po-Kang Lin
  • Patent number: 10214758
    Abstract: A method of digesting a lignocellulosic material is disclosed. In one embodiment, the method comprises the step of exposing the material to an effective amount of Streptomyces sp. ActE secretome such that at least partial lignocellulosic digestion occurs.
    Type: Grant
    Filed: December 10, 2012
    Date of Patent: February 26, 2019
    Assignee: Wisconsin Alumni Research Foundation
    Inventors: Brian Grant Fox, Taichi Takasuka, Adam Joel Book, Cameron Robert Currie
  • Patent number: 10214759
    Abstract: The present invention pertains to an in vitro method in which the frequency of the targeted nucleotide sequence containing the DNA fragment of interest is increased stepwise, by several rounds of 1) dilution of a sample containing the DNA fragment of interest into several replicates (separation), 2) randomly amplifying DNA in the replicates (concentration), 3) detecting the DNA fragment of interest in at least one of the diluted and amplified replicates (selection) and repeating steps 1) through 3) until the DNA fragment of interest can be sequenced by standard sequencing techniques.
    Type: Grant
    Filed: December 20, 2013
    Date of Patent: February 26, 2019
    Assignee: Samplix ApS
    Inventors: Thomas Kvist, Marie Just Mikkelsen
  • Patent number: 10214760
    Abstract: Disclosed are methods for amplifying a nucleic acid target region using an amplification oligomer comprising a target-binding segment and a heterologous displacer tag situated 5? to the target-binding segment. Initiation of an amplification reaction from the tagged amplification oligomer produces an amplicon comprising the displacer tag, such that once the complement of the displacer tag has been incorporated into a second amplicon, a displacer oligonucleotide having a sequence substantially corresponding to the displacer tag sequence is used to participate in subsequent rounds of amplification for displacement of an extension product primed from a site within the second amplicon 5? to the displacer priming site. Also disclosed are related kits and reaction mixtures comprising the displacer-tagged amplification oligomer and corresponding displacer oligonucleotide.
    Type: Grant
    Filed: October 30, 2015
    Date of Patent: February 26, 2019
    Assignee: GEN-PROBE INCORPORATED
    Inventors: Norman C. Nelson, Margarita Batranina-Kaminsky
  • Patent number: 10214761
    Abstract: There are provided amino acid derivatives of formula V and VI as defined herein which are pyrrolysine analogs for use in bioconjugation processes.
    Type: Grant
    Filed: June 1, 2017
    Date of Patent: February 26, 2019
    Assignee: MEDIMMUNE LIMITED
    Inventors: Kenneth H. Grabstein, Michael Van Brunt, Marcello Marelli
  • Patent number: 10214762
    Abstract: Methods, devices, and kits are provided for identifying, selecting, and/or manipulating live cells in a sample comprising a suspected mixed population of cells by subjecting the sample located in a first position to a linear gradient of a cellular stimulus to induce a change in a live cell sufficient to induce movement of the cell from a first position to a second position, wherein a dead cell, if present in the sample, is not induced to move to the second position in the presence of the cellular stimulus; and identifying or selecting the live cell in the second position.
    Type: Grant
    Filed: October 28, 2015
    Date of Patent: February 26, 2019
    Assignee: Georgia Tech Research Corporation
    Inventors: Peter J. Hesketh, Douglas Britton, Wayne D. Daley, Alireza Mahdavifar, Jie Xu
  • Patent number: 10214763
    Abstract: A system and method for antibiotic susceptibility testing efficiently determines whether bacteria are alive or have been killed by antibiotic treatment. The antibiotic susceptibility testing device includes at least one reservoir into which a bacteria solution is introduced and a microfluidic channel connected to the reservoir, wherein the cross-sectional size of the microfluidic channel is selected to be comparable to the size of the bacterium to be tested. Furthermore, the electrical resistance or voltage signal across the microchannel is monitored as bacteria swim into and out of the channel. Alternatively, a small population of bacteria can be immobilized in the microchannel. The resistance or voltage signal fluctuates when the bacteria are alive and moving in and out of the channel or wiggling on the microchannel walls. If the bacteria are dead, they have limited motility and the signal fluctuations are significantly smaller.
    Type: Grant
    Filed: February 2, 2018
    Date of Patent: February 26, 2019
    Assignee: TRUSTEES OF BOSTON UNIVERSITY
    Inventors: Kamil Ekinci, Le Li, Chuanhua Duan, Vural Kara, Deborah Stearns-Kurosawa
  • Patent number: 10214764
    Abstract: The present invention is directed to a separation device or container that can be used in the separation, isolation or pelleting of microorganisms from a test samples known to contain or suspected of containing said microorganisms. Subsequently, the separated, isolated or pelleted microorganism sample can undergo one or more interrogation steps to provide measurements useful for the characterization and/or identification of microorganism. In one aspect of the present invention, the interrogation steps can occur in situ in the separation device or container described herein.
    Type: Grant
    Filed: October 13, 2015
    Date of Patent: February 26, 2019
    Assignee: BIOMERIEUX, INC.
    Inventors: John Walsh, Jones M. Hyman, Christopher Ronsick, John Link, Ron Robinson, Mark Wilson
  • Patent number: 10214765
    Abstract: Provided herein are compounds for use as sialidase inhibitors, including alkynyl-3-fluorosialyl fluoride. The compounds, which include the compound DFSA, function by trapping a 3-fluorosialylenzyme intermediate (reporter-inhibitor-enzyme conjugate). These compounds can be conjugated with a detectable tagging moiety for isolation and identification of sialidases.
    Type: Grant
    Filed: January 26, 2018
    Date of Patent: February 26, 2019
    Assignee: Academia Sinica
    Inventors: Chi-Huey Wong, Jim-Min Fang, Yih-Shyun E. Cheng, Chamg-Sheng Tsai
  • Patent number: 10214766
    Abstract: The invention relates to a bioluminescent substrate suitably usable in a series of artificial luciferases (ALuc), and the invention provides a wavelength-shifted spectrum with a selective high intensity luminescence and high luminescence stability obtained by the use of the substrate together with ALuc. The luminescent substrate for ALuc obtained by the invention can be included together with a suitable luminescence solution in a luminescence kit. The bioluminescent substrate for ALuc of the invention can exhibit unprecedented excellent luminescence specificity and functionality in the conventional bioluminescence probe, two-hybrid assay, bioluminescent capsule, and reporter gene assay.
    Type: Grant
    Filed: October 16, 2014
    Date of Patent: February 26, 2019
    Assignee: NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY
    Inventors: Sung Bae Kim, Hiroshi Izumi, Hiroaki Tao, Masaki Torimura, Akihiro Wakisaka
  • Patent number: 10214767
    Abstract: A parallel processing system for processing samples is described. In one embodiment, the parallel processing system includes an instrument interface parallel controller to control a tray motor driving system, a close-loop heater control and direction system, a magnetic particle transfer system, a reagent release system, a reagent pre-mix pumping system and a wash buffer pumping system.
    Type: Grant
    Filed: August 8, 2018
    Date of Patent: February 26, 2019
    Assignee: LUMINEX CORPORATION
    Inventors: Steve Jia Chang Yu, Jesus Ching, Phillip You Fai Lee, David Hsiang Hu
  • Patent number: 10214768
    Abstract: The present application relates to new coumarin compounds and their uses as fluorescent labels. The compounds may be used as fluorescent labels for nucleotides in nucleic acid sequencing applications.
    Type: Grant
    Filed: December 21, 2017
    Date of Patent: February 26, 2019
    Assignee: Illumina Cambridge Limited
    Inventor: Nikolai Romanov
  • Patent number: 10214769
    Abstract: Provided is a probe to be used in a DNA microarray having an excellent detection rate of a polymorphism such as SNP contained in genomic DNA. A method for designing a probe according to the invention includes the steps of: specifying one or more regions covering at least a part of fragments flanked by restriction enzyme recognition sites recognized by a restriction enzyme, contained in genomic DNA derived from an organism to be tested; and designing a probe for the specified one or more regions for detecting the fragment in the organism to be tested.
    Type: Grant
    Filed: February 28, 2017
    Date of Patent: February 26, 2019
    Assignee: TOYOTA JIDOSHA KABUSHIKI KAISHA
    Inventors: Hiroyuki Enoki, Satoru Nishimura, Aya Murakami
  • Patent number: 10214770
    Abstract: Compositions and methods are described for standardizing the DNA amplification efficiencies of a highly heterogeneous set of oligonucleotide primers as may typically be used to amplify a heterogeneous set of DNA templates that contains rearranged lymphoid cell DNA encoding T cell receptors (TCR) or immunoglobulins (IG). The presently disclosed embodiments are useful to overcome undesirable bias in the utilization of a subset of amplification primers, which leads to imprecision in multiplexed high throughput sequencing of amplification products to quantify unique TCR or Ig encoding genomes in a sample. Provided is a composition comprising a diverse plurality of template oligonucleotides in substantially equimolar amounts, for use as a calibration standard for amplification primer sets. Also provided are methods for identifying and correcting biased primer efficiency during amplification.
    Type: Grant
    Filed: May 10, 2016
    Date of Patent: February 26, 2019
    Assignee: ADAPTIVE BIOTECHNOLOGIES CORP.
    Inventors: Harlan Saul Robins, Christopher Scott Carlson, Robert J. Livingston, Ryan O. Emerson, Anna M. Sherwood
  • Patent number: 10214771
    Abstract: The present invention is directed to methods to prepare a DNA molecule or a plurality of DNA molecules by random fragmentation. In some embodiments, the present invention regards preparing a template for DNA sequencing by random fragmentation. In specific embodiments, the random fragmentation comprises chemical fragmentation, mechanical fragmentation, or enzymatic fragmentation. In further specific embodiments, a universal sequence is attached to the 3? end of the DNA fragments, such as by ligation of an adaptor sequence or by homopolymeric tailing with terminal deoxynucleotidyltransferase. In other embodiments, a library is prepared with methods of the present invention.
    Type: Grant
    Filed: August 5, 2016
    Date of Patent: February 26, 2019
    Assignee: Takara Bio USA, Inc.
    Inventors: Vladimir L. Makarov, Irina Sleptsova, Emmanuel Kamberov, Eric Bruening
  • Patent number: 10214772
    Abstract: A disposable test card configured to accept a fluid sample for an assay, and a method of manufacturing same, is disclosed herein. In a general example embodiment, a test card for analyzing a fluid sample includes a first substrate layer including an inlet port and an outlet port, a channel layer bonded to the first substrate layer, the channel layer including a microchannel placing the inlet port in fluid communication with the outlet port, and a second substrate layer bonded to the channel layer, the second substrate layer having electrodes printed adjacent to a target zone of the microchannel of the channel layer, wherein the electrodes are configured to raise the temperature of the fluid sample within the target zone of the microchannel when a current is applied thereto.
    Type: Grant
    Filed: June 17, 2016
    Date of Patent: February 26, 2019
    Assignee: FluxErgy, LLC
    Inventors: Ryan Alan Revilla, Roy James Heltsley, Steve Hoe Lee, Tej Rushikesh Patel
  • Patent number: 10214773
    Abstract: Methods, reagents, and kits for detection and analysis of nucleic acids are provided. The methods can be used in conjunction with a proximity extension assay for protein detection to provide a multiplex assay to detect both nucleic acids (e.g., RNA) and proteins.
    Type: Grant
    Filed: October 3, 2017
    Date of Patent: February 26, 2019
    Assignee: Fluidigm Corporation
    Inventor: Robert C. Jones
  • Patent number: 10214774
    Abstract: The present invention relates to a novel method for analyzing nucleic acid sequences based on real-time detection of DNA polymerase-catalyzed incorporation of each of the four nucleotide bases, supplied individually and serially in a microfluidic system, to a reaction cell containing a template system comprising a DNA fragment of unknown sequence and an oligonucleotide primer. Incorporation of a nucleotide base into the template system can be detected by any of a variety of methods including but not limited to fluorescence and chemiluminescence detection. Alternatively, microcalorimetic detection of the heat generated by the incorporation of a nucleotide into the extending template system using thermopile, thermistor and refractive index measurements can be used to detect extension reactions.
    Type: Grant
    Filed: May 2, 2014
    Date of Patent: February 26, 2019
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventors: Peter Williams, Daniel J. B. Williams, Linda Bloom, Thomas J. Taylor
  • Patent number: 10214775
    Abstract: The invention provides methods and reagents for diagnosing prostate cancer that are based on the detection of biomarkers in the circulating nucleic acids from a patient to be evaluated.
    Type: Grant
    Filed: December 7, 2012
    Date of Patent: February 26, 2019
    Assignee: Chronix Biomedical
    Inventors: Ekkehard Schutz, Julia Beck, Howard Urnovitz
  • Patent number: 10214776
    Abstract: The present application relates to methods of detecting a mutation in a target nucleic acid molecule. Two phosphorodiamidate morpholino oligomer probes that differ by at least one base are each covalently coupled to a nano article and hybridized to a target sequence. The melting temperature of the complexes between each of the two probes and the target nucleic acid are measured and compared to determine whether the sample contains a nucleic acid with the mutation. Further, the present invention relates to kits comprising a first and second conjugate as described herein and to the use of such kits for the detection of mutations in a target nucleic acid molecule or for assigning a genotype to a target nucleic acid molecule.
    Type: Grant
    Filed: April 4, 2014
    Date of Patent: February 26, 2019
    Assignee: Agency for Science, Technology and Research
    Inventors: Yanbing Zu, Jackie Y. Ying
  • Patent number: 10214777
    Abstract: Methods and compositions are provided for the identification of a molecular diagnostic test for cancer. The test defines a novel DNA damage repair deficient molecular subtype and enables classification of a patient within this subtype. The present invention can be used to determine whether patients with cancer are clinically responsive or non-responsive to a therapeutic regimen prior to administration of any chemotherapy. This test may be used in different cancer types and with different drugs that directly or indirectly affect DNA damage or repair, such as many of the standard cytotoxic chemotherapeutic drugs currently in use. In particular, the present invention is directed to the use of certain combinations of predictive markers, wherein the expression of the predictive markers correlates with responsiveness or non-responsiveness to a therapeutic regimen.
    Type: Grant
    Filed: August 13, 2015
    Date of Patent: February 26, 2019
    Assignee: Almac Diagnostics Limited
    Inventors: Jude O'Donnell, Max Bylesjo, Fionnuala Patterson, Steve Deharo, Laura A. Hill, Katherine E. Keating, Timothy Davison, Vitali Proutski, Denis Paul Harkin, Richard Kennedy, Nicolas Goffard
  • Patent number: 10214778
    Abstract: Provided in the present invention are a method using in vitro measurement of the content of methylation or demethylation of GFRa1 CpG islands to estimate a risk of tumorigenesis and of tumor metastasis, or postoperative life expectancy, and a nucleotide sequence used.
    Type: Grant
    Filed: February 21, 2017
    Date of Patent: February 26, 2019
    Assignee: Beijing Institute For Cancer Research
    Inventors: Dajun Deng, Jun Zhang, Zhaojun Liu, Jing Zhou, Liankun Gu, Baozhen Zhang
  • Patent number: 10214779
    Abstract: The present invention relates to methods for the identification of anti-HIV miRNAs and anti-HIV pharmaceutical compounds using high-throughput screening methods, comprising: transfecting reporter cells with a panel of miRNAs, infecting the reporter cells with HIV, screening the cells to identify miRNAs that modulate HIV infection and identifying the specific pathways, nucleic acids and/or polypeptides that are targeted by the miRNAs. The invention further provides for the identification and screening of anti-HIV pharmaceutical compounds having known activity against the specific pathways, nucleic acids and/or polypeptides that are targeted by the miRNAs for efficacy in the treatment of HIV. The invention also provides for the use of miRNA mimics, miRNA inhibitors and pharmaceutical compounds (including oncology drugs and kinase inhibitors) in the treatment and/or prevention of HIV infection.
    Type: Grant
    Filed: September 1, 2017
    Date of Patent: February 26, 2019
    Assignee: CSIR
    Inventors: Jerolen Naidoo, Samantha Barichievy, Rethabile Khutlang, Musa M Mhlanga
  • Patent number: 10214780
    Abstract: The present invention is concerned with a method of rapid identification of a mammalian species origin or mammalian species origins of a sample.
    Type: Grant
    Filed: November 10, 2016
    Date of Patent: February 26, 2019
    Assignee: CITY UNIVERSITY OF HONG KONG
    Inventors: Shuk Han Cheng, Chun Chi Lin
  • Patent number: 10214781
    Abstract: A method of identifying a subject having a urinary tract infection posing a significant risk of dangerous sequalae is described. The method includes obtaining a urine or fecal sample from the subject; determining the predominant LPS O-antigen serotype in the sample; and comparing the predominant LPS O-antigen serotype to a set of febrile UTI LPS serotypes. The method can also include treating the subject for UTI if the predominant O-antigen LPS serotype is a febrile UTI LPS serotype.
    Type: Grant
    Filed: June 13, 2016
    Date of Patent: February 26, 2019
    Assignee: RESEARCH INSTITUTE AT NATIONWIDE CHILDREN'S HOSPITAL
    Inventors: Sheryl S. Justice, Kevin M. Mason
  • Patent number: 10214782
    Abstract: Embodiments of the invention provide a method of detecting one or more strains of Klebsiella pneumoniae. The method may include forming a plurality of mixtures for nucleic amplification. The method can include amplification of specific sequences within the K. pneumonia genome that can provide definitive information to distinguish between one or more types or strains of K. pneumonia.
    Type: Grant
    Filed: July 20, 2016
    Date of Patent: February 26, 2019
    Assignees: The Translational Genomics Research Institute, Arizona Board of Regents on Behalf of Northern Arizona University
    Inventors: Jolene Bowers, Elizabeth Driebe, David Engelthaler, Paul Keim
  • Patent number: 10214783
    Abstract: A method is provided for determining the zygosity of an Rf4 gene in a corn plant. A method may include performing a first PCR assay, a second PCR assay, quantifying probes used in the first and second PCR assays, and comparing the quantified probes to determine zygosity.
    Type: Grant
    Filed: July 23, 2013
    Date of Patent: February 26, 2019
    Assignee: Dow AgroSciences LLC
    Inventors: Ruihua Ren, Peizhong Zheng, Siva Prasad Kumpatla
  • Patent number: 10214784
    Abstract: The present invention relates to methods and compositions for identifying, selecting and/or producing a maize plant or plant part having increased fertility. A maize plant or plant part that has been identified, selected and/or produced by any of the methods of the present invention is also provided.
    Type: Grant
    Filed: February 20, 2015
    Date of Patent: February 26, 2019
    Assignee: Syngenta Participations AG
    Inventors: Steven William Ritchie, Satya P. Chintamanani, Molly Dunn, Elhan Sultan Ersöz, David Jay Foster, Nicolas Federico Martin, David Stewart Skibbe, Dominic Michael Tucker
  • Patent number: 10214785
    Abstract: The present disclosure provides adeno-associated virus (AAV) virions with altered capsid protein, where the AAV virions exhibit greater infectivity of retinal cells, when administered via intravitreal injection, compared to wild-type AAV. The present disclosure further provides methods of delivering a gene product to a retinal cell in an individual, and methods of treating ocular disease.
    Type: Grant
    Filed: August 23, 2016
    Date of Patent: February 26, 2019
    Assignee: The Regents of the University of California
    Inventors: David V. Schaffer, Ryan R. Klimczak, James T. Koerber, John G. Flannery, Deniz Dalkara Mourot, Meike Visel, Leah C. T. Byrne
  • Patent number: 10214786
    Abstract: A method of producing microbial biomass include providing a wastewater stream and determining the concentration of micronutrients selected from the group consisting of aluminum, boron, cobalt, magnesium, manganese, and zinc, and any combination thereof, in the wastewater stream. The method also includes determining the biological oxygen demand (BOD) normalized dose of the micronutrients, and modulating the concentration of at least one micronutrient in the wastewater stream to provide a micronutrient-modulated wastewater stream. The method further includes growing microbial biomass in the micronutrient-modulated wastewater stream.
    Type: Grant
    Filed: October 4, 2013
    Date of Patent: February 26, 2019
    Assignee: PROCELL INVESTMENTS LIMITED
    Inventors: Andrew J. Logan, Kathryn R. Spangler
  • Patent number: 10214787
    Abstract: The invention relates to a hot-briquetting or hot-compacting installation, in particular for directly reduced iron, having at least one first row (1) of presses with one or more first roll presses (2), and having at least one second row (3) of presses with one or more second roll presses (4), having at least one first cooling conveyor (7) beneath the first row (1) of presses, and a second cooling conveyor (8) beneath the second row (3) of presses, and having a plurality of material lines (6), which are assigned in each case to the individual roll presses (2, 4) and via which the briquetted or compacted material exiting from the roll presses (2, 4) is discharged to the cooling conveyors (7, 8). The material lines (6) are provided with one or more diverters (9), which can be adjusted such that the material from the roll presses (2, 4) of the first row (1) of presses and of the second row (3) of presses is discharged optionally in each case to the first cooling conveyor (7) or to the second cooling conveyor (8).
    Type: Grant
    Filed: May 13, 2015
    Date of Patent: February 26, 2019
    Assignee: MASCHINENFABRIK KOEPPERN GMBH & CO. KG
    Inventors: Christopher Schaefer, Eggert De Weldige
  • Patent number: 10214788
    Abstract: Disclosed herein are methods and compositions for producing composite iron pellets comprising an inner core comprising iron ore and a reducing agent comprising a carbonaceous material; and an outer shell comprising unreduced iron ore. The resulting composite iron pellets can be used to produce direct reduced iron (DRI) with improved productivity while reducing gas consumption.
    Type: Grant
    Filed: January 28, 2015
    Date of Patent: February 26, 2019
    Assignee: SAUDI BASIC INDUSTRIES CORPORATION
    Inventor: Mohamed Bahgat Saddik
  • Patent number: 10214789
    Abstract: Quench plug systems and their use in heat treating a workpiece. The quench plug system includes a tapered plug having a longitudinal core axis and a mandrel configured to be interposed between the tapered plug and the workpiece. The tapered plug is configured to allow the mandrel to translate along the core axis of the tapered plug when the workpiece is heated.
    Type: Grant
    Filed: September 16, 2015
    Date of Patent: February 26, 2019
    Assignee: The Boeing Company
    Inventors: Matthew D. Carter, Richard G. Kummerle, David Salber
  • Patent number: 10214790
    Abstract: A method is disclosed for producing components from an austenitic lightweight steel which is metastable in its initial state, by forming of a sheet, a circuit board or a pipe in one or more steps, exhibiting a temperature-dependent TRIP and/or TWIP effect during forming. To obtain a component with, in particular, high toughness, the forming is carried out at a temperature above room temperature, at 40 to 160° C., which avoids the TRIP/TWIP effect, and to achieve in particular high component strength, the forming is carried out at a temperature below room temperature, at ?65 to 0° C., which enhances the TRIP/TWIP effect.
    Type: Grant
    Filed: May 6, 2013
    Date of Patent: February 26, 2019
    Assignee: Salzgitter Flachstahl GmbH
    Inventors: Thomas Evertz, Manuel Otto, Bianca Springub, Zacharias Georgeou
  • Patent number: 10214791
    Abstract: A non-oriented electrical steel sheet containing: in mass%, C: 0.005% or less; Si: 0.1% to 2.0%; Mn: 0.05% to 0.6%; P: 0.100% or less; and Al: 0.5% or less, in which 10 pieces/?m3 or less in number density of non-magnetic precipitate AlN having an average diameter of 10 nm to 200 nm are contained, and an average magnetic flux density B50 in a rolling direction and in a direction perpendicular to rolling is 1.75 T or more. This non-oriented electrical steel sheet can be manufactured by two methods of a method of performing hot rolling annealing at a temperature of 750° C. to an Ac1 transformation point and a method of setting a coil winding temperature to 780° C. or higher and performing self annealing.
    Type: Grant
    Filed: May 30, 2017
    Date of Patent: February 26, 2019
    Assignee: NIPPON STEEL & SUMITOMO METAL CORPORATION
    Inventors: Masahiro Fujikura, Yoshiyuki Ushigami, Tesshu Murakawa, Shinichi Kanao, Makoto Atake, Takeru Ichie, Kojiro Hori
  • Patent number: 10214792
    Abstract: The invention relates to a hot-rolled steel sheet having a tensile strength greater than 800 MPa and an elongation at break greater than 10%, the composition of which comprises, the contents being expressed by weight: 0.050%?C?0.090%, 1%?Mn?2%, 0.015%?Al?0.050%, 0.1%?Si?0.3%, 0.10%?Mo?0.40%, S?0.010%, P?0.025%, 0.003%?N?0.009%, 0.12%?V?0.22%, Ti?0.005%, Nb?0.020% and, optionally, Cr?0.45%, the balance of the composition consisting of iron and inevitable impurities resulting from the smelting, the microstructure of the sheet or the part comprising, as a surface fraction, at least 80% upper bainite, the possible complement consisting of lower bainite, martensite and residual austenite, the sum of the martensite and residual austenite contents being less than 5%.
    Type: Grant
    Filed: December 18, 2014
    Date of Patent: February 26, 2019
    Assignee: ArcelorMittal France
    Inventors: Pascal Drillet, Damien Ormston
  • Patent number: 10214793
    Abstract: Provided is a method for nitriding a grain-oriented electrical steel sheet which is very useful in obtaining excellent magnetic properties with no variation by immersing a strip in a molten salt bath after cold rolling and before secondary recrystallization annealing during a production process of a grain-oriented electrical steel sheet, to subject the strip to continuous nitriding to uniformly disperse inhibitor forming elements over the full length and full width of the strip.
    Type: Grant
    Filed: February 18, 2014
    Date of Patent: February 26, 2019
    Assignee: JFE STEEL CORPORATION
    Inventors: Hiroshi Matsuda, Hideyuki Takahashi, Hiroi Yamaguchi, Yukihiro Shingaki, Yasuyuki Hayakawa, Takashi Terashima