Abstract: Provided herein are methods of diagnosing or monitoring the treatment of abnormal glycan accumulation or a disorder associated with abnormal glycan accumulation.

Abstract: In one form, a diagnostic element for determining at least one analyte is provided. In a further form, an analytical measuring device includes the diagnostic element. Still, other forms are related to methods for the determination of an analyte, correcting a signal generated by an analyte, and/or checking the detection optics of an analytical measuring device using the diagnostic element. Another form is related to a system for the controlled release of a reagent and the use of such a system as a circuit element. Other aspects include, but are not limited to, unique methods, techniques, products, systems and devices involving diagnostic elements.

Abstract: The invention relates to a nanoscale antenna including a nucleic acid scaffold having a structure selected from the group consisting of a Holliday junction, a star, and a dendrimer; and a plurality of fluorophores attached to the scaffold and configured as a FRET cascade comprising at least three different types of fluorophores including at least one quantum dot, arranged with (a) a plurality of initial donor fluorophores fixed in exterior positions on the structure, (b) a terminal acceptor fluorophore fixed in a central position on the structure, and (c) a plurality of intermediate fluorophores fixed in positions on the scaffold between the initial acceptor fluorophores and the terminal acceptor fluorophores.

Abstract: The present invention relates to methods for the identification of methylated cytosines in a population of double stranded DNA molecules. The invention also relates to adapters and kits for synthesizing said adapters as well as to double stranded DNA libraries obtained by the methods of the invention.

Abstract: Methods and compositions are provided for identifying any of the presence, location and phasing of methylated and/or hydroxymethylated cytosines in nucleic acids including long stretches of DNA. In some embodiments, the method may comprise reacting a first portion (aliquot) of a nucleic acid sample with a dioxygenase and optionally a glucosyltransferase in a reaction mixture containing the nucleic acid followed by a reaction with a cytidine deaminase to detect and optionally map 5mC in a DNA. Optionally, a second portion can be reacted with glucosyltransferase followed by reaction with a cytidine deaminase to detect and optionally map 5hmC in a DNA.

Abstract: Peptide nucleic acids containing thymidine and 2-aminopyridine (M) nucleobases formed stable and sequence selective triple helices with double stranded RNA at physiologically relevant conditions. The M-modified PNA displayed unique RNA selectivity by having two orders of magnitude higher affinity for the double stranded RNAs than for the same DNA sequences. Preliminary results suggested that nucleobase-modified PNA could bind and recognize double helical precursors of microRNAs.

Abstract: A method of synthesizing a nucleic acid complementary to a target nucleic acid sequence in a template nucleic acid includes annealing a swarm primer to a target nucleic acid, the swarm primer overlapping an F1 site of the target nucleic acid and extends toward the F2 site of the target nucleic acid. An inner primer may also be annealed to the target nucleic acid to produce a complimentary nucleic acid having a single-strand loop onto which further primers may anneal. A plurality of amplicons may be reproduced, many of which may have further primers annealed thereto to generate more complementary nucleic acids.

Abstract: An analysis unit for performing a polymerase chain reaction includes a cover element, a bottom element, at least one fluid channel, at least one pressure channel, and a film. The bottom element has at least one bottom hollow that defines a fluid-accommodating surface and an arrangement of microcavities, and that faces toward the cover element. The fluid channel is formed between the cover element and the bottom element in order to conduct a fluid onto the fluid-accommodating surface. The pressure channel is formed in the cover element in order to conduct a pressure into a region of the fluid-accommodating surface. The film is positioned between the cover element and the bottom element in a region of the bottom hollow, and is configured to deform in response to the pressure conducted through the pressure channel such that the fluid is moved through the fluid-accommodating surface into the arrangement of microcavities.

Abstract: Systems and methods of polynucleotide sequencing are provided. Systems and methods optimize control, speed, movement, and/or translocation of a sample (e.g., a polynucleotide) within, through, or at least partially through a nanopore or a type of protein or mutant protein in order to accumulate sufficient time and current blocking information to identify contiguous nucleotides or plurality of nucleotides in a single-stranded area of a polynucleotide.

Abstract: This invention provides a process for sequencing nucleic acids using 3? modified deoxynucleotide analogs or 3? modified deoxyinosine triphosphate analogs, and 3? modified dideoxynucleotide analogs having a detectable marker attached to a base thereof.

Abstract: The invention relate to systems and methods for sequencing polynucleotides, as well as detecting reactions and binding events involving other biological molecules. The systems and methods may employ chamber-free devices and nanosensors to detect or characterize such reactions in high-throughput. Because the system in many embodiments is reusable, the system can be subject to more sophisticated and improved engineering, as compared to single use devices.

Abstract: Disclosed herein is a system and method for increasing the fidelity of measured genetic data, for making allele calls, and for determining the state of aneuploidy, in one or a small set of cells, or from fragmentary DNA, where a limited quantity of genetic data is available. Poorly or incorrectly measured base pairs, missing alleles and missing regions are reconstructed using expected similarities between the target genome and the genome of genetically related individuals. In accordance with one embodiment, incomplete genetic data from an embryonic cell are reconstructed at a plurality of loci using the more complete genetic data from a larger sample of diploid cells from one or both parents, with or without haploid genetic data from one or both parents. In another embodiment, the chromosome copy number can be determined from the measured genetic data, with or without genetic information from one or both parents.

Abstract: Methods and compositions are provided for the identification of a molecular diagnostic test for cancer. The test identifies cancer subtypes that are responsive to anti-angiogenesis therapeutics and enables classification of a patient within this subtype. The present invention can be used to determine whether patients with cancer are clinically responsive or non-responsive to a therapeutic regimen prior to administration of any anti-angiogenic agent. This test may be used in different cancer types and with different drugs that directly or indirectly affect angiogenesis or angiogenesis signalling. In addition, the present invention may be used as a prognostic indicator for certain cancer types. In particular, the present invention is directed to the use of certain combinations of predictive markers, wherein the expression of the predictive markers correlates with responsiveness or non-responsiveness to a therapeutic regimen.

Abstract: The present invention relates to neurological disease, in particular to mutations in the FIG4 gene. The present invention also provides assays for the detection of variant FIG4 alleles, and assays for detecting FIG4 polymorphisms and mutations associated with disease states such as ALS.

Abstract: Compositions and methods for diagnosis or treatment of epilepsy disease with EFHC1, EFHC1 agonists, or EFHC1 analogs are provided. Compositions and methods for diagnosis or treatment of epilepsy disease with EFHC1a, EFHC1a agonists, or EFHC1a analogs are provided.

Abstract: The present invention relates to neuropathy, in particular to mutations in the FIG4 gene. The present invention also provides assays for the detection of variant FIG4 alleles, and assays for detecting FIG4 polymorphisms and mutations associated with disease states.

Abstract: The present invention provides methods of detecting c-KIT-dependent-melanoma for diagnostic and prognostic purposes. The invention further provides methods of treating such melanoma by inhibiting c-KIT.

Abstract: The invention comprises reagents and methods for detecting cancer-associated mutations in the human EZH2 gene. Further, a method of detecting the mutations and a method of treatment are disclosed.

Abstract: The present invention provides dignostic assays for identifying thyroid cancer in a biological sample, including a fine needle aspirate, as well as related compositions and kits useful in practicing the methods of the invention.

Abstract: The present disclosure includes assays that involve measurement of expression levels of prognostic biomarkers, or co-expressed biomarkers, from a biological sample obtained from a prostate cancer patient, and analysis of the measured expression levels to provide information concerning the likely prognosis for the patient, and likelihood that the patient will have a recurrence of prostate cancer, or to classify the tumor by likelihood of clinical outcome or TMPRSS2 fusion status.

Abstract: Embodiments provide methods and compositions related to determining treatments for colorectal cancer patients by detection and analysis of the expression level of miRNA such as miR-320e in the patients. Embodiments provide predictive, prognostic and/or diagnostics methods by identifying miRNAs that are useful for clinical management of cancer patients, particularly colorectal cancer patients or patients at risk or determined to have colorectal cancer. Methods and compositions are based, in part, on the discovery that expression of certain miRNAs in cancer patients is associated with advancing cancer stages and/or can predict the responsiveness of cancer therapy, and can, therefore, provide basis for designing treatment strategies. In particular embodiments, the miRNA molecule is miR-320, particularly miR-320e.

Abstract: A method for diagnosing and treating prostate cancer in a human subject is provided, the method comprises: a. providing a sample of prostate tissue, blood, or urine from the subject; b. determining the level of expression of SEQ ID NO: 1 and the expression of SEQ ID NO: 5 in the sample, wherein an increased level of expression of SEQ ID NO:1 and a reduced level of expression of SEQ ID NO: 5 in the sample is indicative of a diagnosis that the subject has prostate cancer; and c. administering a therapeutically effective prostate cancer treatment selected from the group consisting of an androgen receptor (AR)-targeted therapy, an antimicrotubule agent, an alkylating agent and an anthracenedione to the subject to treat the prostate cancer in the human subject diagnosed with prostate cancer.

Abstract: Methods of screening a sample for a predisposition for forming an EGFR-associated cancer and the presence of EGFR (v3) contained therein are described. The methods include the steps of obtaining a sample containing a plurality of cells and hybridizing a first set of labeled chromosomal probes and a second set of labeled chromosomal probes to the sample. The first set of labeled chromosomal probes specific to chromosome 7, and the second set of labeled chromosomal probes is a labeled EGFR(v3)-probe. The first and second sets of labeled chromosomal probes include different labels to permit identification of the first and second sets of labeled chromosomal probes hybridized to the sample. The method includes calculating the number of signals of the first and second sets labeled chromosomal probes on an individual cell basis and determining a predisposition for forming an EGFR-associated cancer and the presence of EGFR (v3) contained therein based upon those calculations.

Abstract: A method is provided for selecting a subject diagnosed with cancer as a candidate for treatment with a PARP1 inhibitor, or both a PARP1 inhibitor and a chemotherapeutic agent such as an alkylating agent. The method includes detecting the presence or absence of a mutation in a non-coding region of a PARP1 gene, wherein the presence of a mutation in the PARP1 gene indicates that the cancer can be treated with the PARP1 inhibitor, optionally in conjunction with the chemotherapeutic agent. In a specific, non-limiting example, the PARP1 inhibitor is verliparib (ABT-888).

Abstract: This disclosure is in the field of molecular biology and medical diagnosis and provides means and methods for determining the prognosis and disease outcome of a subject having a melanoma. More in particular, this disclosure provides a method for determining whether a subject having a melanoma has a poor prognosis, the method comprising the step of determining in a sample from the subject whether the LY75 promoter is methylated and if the LY75 promoter is methylated, classifying the subject as having a poor prognosis.

Abstract: The present invention provides an artificial exogenous reference molecule for type and abundance comparison among different species of microorganisms. The artificial exogenous reference molecule includes several species detection regions and several linker sequence regions, where the species detection regions and the linker sequence regions are arranged alternately; at least two species detection regions are provided and are species detection regions for different species; and the linker sequence regions are random sequences different from species sequences to be detected. The species detection region includes a primer sequence region and a specific recognition region; the sequence length of the specific recognition region simulates the characteristic detection sequence length of the corresponding species to be detected; and the sequence of the specific recognition region has obviously specific difference from the DNA sequence of a genome in the species to be detected without homology.

Abstract: This disclosure provides a method for improving the efficiency and timing of detecting whether sepsis-related microorganisms are present in a fluid sample. The method comprises the steps of: collecting the fluid sample from a patient; fractioning the fluid sample to isolate a quantity of microorganism cells; extracting a portion of the microorganism cells from the fluid sample; lysing a portion of the microorganism cells extracted from the fluid sample to extract microorganism DNA; amplifying the microorganism DNA from the microorganism cells from a predetermined set of DNA primers to determine whether sepsis-related microorganisms are present within the fluid sample.

Abstract: The present invention discloses novel oligonucleotide primer sequences BC1, BC2 and BC3 for the identification of Bacillus coagulans. The invention also discloses a PCR based method for the identification of Bacillus coagulants using the aforesaid primers, wherein positive amplification with primer sets BC1, BC2 and negative amplification with primer set BC3 confirms the presence of Bacillus coagulans.

Abstract: The present invention generally relates to a molecular test of Influenza A, Influenza B, Respiratory Syncytial Virus A, and Respiratory Syncytial Virus B in order to identify patients with a viral infection. Accordingly methods and compositions are disclosed to determine the presence or absence of a viral pathogen in a sample containing one or more target nucleic acids from the M gene of Influenza A, Influenza B, Respiratory Syncytial Virus A, and/or Respiratory Syncytial Virus B.

Abstract: The present invention relates in a first aspect to a method of preparing a crystalline lanthanum-carboxylate coordination polymer and the crystalline lanthanum-carboxylate coordination polymer obtained or obtainable by the method. In another aspect of the present invention, also provides a method of detecting a target nucleic acid sequence in a sample.

Abstract: A process for leather production from a raw animal hide includes the steps of pre-soaking, soaking, unhairing and liming, re-liming, deliming and bating, pickling and tanning, degreasing, re-tanning, neutralizing, dyeing and fatliquoring, and washing the processed hide. Waste liquid is collected from at least one of the foregoing steps is recycled to at least one of the foregoing steps. The collected waste liquid can be recycled to the same step, to a different step, or to two of more steps in the process.

Abstract: An apparatus for shaving a tap hole surface of a blast furnace or the like wherein efficiency can be improved, and a worker can be freed from physically burdensome work in the vicinity of the lower furnace wall of a blast furnace under high temperature. The apparatus includes a surface shaving unit having smoothing bits, and a rotating mechanism unit that, when the surface shaving unit is pressed against the tap hole surface, rotates the surface shaving unit, by utilizing the pressing force, by a predetermined angle in a predetermined direction substantially parallel to the surface, and holds the surface shaving unit at the rotated position. The rotating mechanism unit eliminates the need for work such that every time a conventional surface shaving unit is separated from the tap hole surface after being pressed against the surface, a worker manually rotates the surface shaving unit by a predetermined angle.

Abstract: In one aspect, the invention relates to a system for producing direct reduced iron wherein a portion of the top gas from a first module for reducing iron oxide by a direct reduction process is utilized as fuel in the thermal equipment of a second module for reducing iron oxide by a direct reduction process, wherein the second module comprises a process gas heating unit. In various aspects, the thermal equipment of the second module is a reducing gas heater and/or a steam boiler. In a further aspect, the top gas from multiple instances of the first module can be utilized collectively as fuel in the thermal equipment of the second module. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present invention.

Abstract: A formed and hardened component made from a metallic material is post-treated by a device for electric resistance heating that has at least one first pair of contact pieces and at least one second pair of contact pieces; contacting a first partial region of the component with the contact pieces of the first pair such that the first partial region is arranged between the contact pieces of the first pair; contacting the second partial region of the component with the contact pieces of the second pair such that the second partial region is arranged between the contact pieces of the second pair; heating of the first partial region of the component to a first temperature by conducting electric current through the component by the first pair of contact pieces; setting the second partial region of the component to a second temperature by the second pair of contact pieces, which is set independently of the first temperature.

Abstract: The present invention relates to a maraging steel containing, in terms of mass %, 0.20?C?0.35, 9.0?Co?20.0, 1.0?(Mo+W/2)?2.0, 1.0?Cr?4.0, and a certain amount of Ni, with the balance being Fe and inevitable impurities, in which in a case where the contents of V and Nb satisfy V+Nb?0.020 mass %, the amount of Ni is 6.0?Ni?9.4, and in which in a case where the contents of V and Nb satisfy 0.020 mass %<V+Nb?0.60 mass %, the amount of Ni is 6.0?Ni?16.0.

Abstract: A process for reducing flatness deviations in an alloy article is disclosed. An alloy article may be heated to a first temperature at least as great as a martensitic transformation start temperature of the alloy. A mechanical force may be applied to the alloy article at the first temperature. The mechanical force may tend to inhibit flatness deviations of a surface of the alloy article. The alloy article may be cooled to a second temperature no greater than a martensitic transformation finish temperature of the alloy. The mechanical force may be maintained on the alloy article during at least a portion of the cooling of the alloy article from the first temperature to the second temperature.

Abstract: In a method for increasing steel impact toughness, the steel composition contains from about 5 wt % to about 10 wt % manganese and has a martensite finish temperature (Mf) below room temperature. The steel composition is exposed to hot forming to form a steel part. During hot forming, the steel composition is subjected to a heat treatment temperature above its fully austenite formed temperature, is transferred to a die, and while in the die, is simultaneously formed and quenched. In one example, quenching cools the steel composition to room temperature, and the steel part is removed from the die and reheated to a baking temperature ranging from about 120° C. to about 400° C. In another example, quenching is interrupted at an interruption temperature ranging from about 120° C. to about 400° C., and the steel composition is maintained at the interruption temperature for a predetermined time and then is cooled to room temperature.

Abstract: This invention pertains to plastic injection mold tooling, and also large forgings, formed from a low carbon mold steel having markedly increased hardening and hardenability properties in large sections as contrasted to currently available commercial products. The above attributes are obtained together with equal or better machinability and improved mold parting line wear. When manufactured in conjunction with a double melt process, this invention can improve significantly polishing characteristics and other attributes of molded parts in tooling sets.

Abstract: A rolled steel bar for machine structural use includes a predetermined chemical composition comprising, by mass %, C: 0.45% to 0.65%, Si: higher than 1.00% to 1.50%, Mn: higher than 0.40% to 1.00%, P: 0.005% to 0.050%, S: 0.020% to 0.100%, V: 0.08% to 0.20%, Ti: 0% to 0.050%; Ca: 0% to 0.0030%, Zr: 0% to 0.0030%, Te: 0% to 0.0030%, and a remainder including Fe and impurities, wherein the impurities include: Cr: 0.10% or lower, Al: lower than 0.01%, and N: 0.0060% or lower. In the rolled steel bar for machine structural use, K1 obtained from “K1=C+Si/7+Mn/5+1.54×V” is 0.95 to 1.05, K2 obtained from “K2=139?28.6×Si+105×Mn?833×S?13420×N” is more than 35, K3 obtained from “K3=137×C?44.0×Si” is 10.7 or more, a Mn content and a S content satisfy Mn/S?8.0, and a total decarburized depth in a surface layer is 500 ?m or less.

Abstract: There is provided a hot-rolled steel sheet in which a composition contains: in mass %, C: 0.01% to 0.2%; Si: 2.5% or less; Mn: 4.0% or less; P: 0.10% or less; S: 0.03% or less; Al: 0.001% to 2.0%; N: 0.01% or less; O: 0.01% or less; Ti: 0.01 to 0.30%; and the balance being composed of iron and impurities and a structure is composed of by volume fraction, 90% or more of tempered martensite with an average aspect ratio of 2 or less, or 90% or more in total of both tempered martensite and lower bainite.

Abstract: There is provided an austenitic stainless steel for high-pressure hydrogen gas consisting, by mass percent, of C: 0.10% or less, Si: 1.0% or less, Mn: 3% or more to less than 7%, Cr: 15 to 30%, Ni: 10% or more to less than 17%, Al: 0.10% or less, N: 0.10 to 0.50%, and at least one kind of V: 0.01 to 1.0% and Nb: 0.01 to 0.50%, the balance being Fe and impurities, wherein in the impurities, the P content is 0.050% or less and the S content is 0.050% or less, the tensile strength is 800 MPa or higher, the grain size number (ASTM E112) is No. 8 or higher, and alloy carbo-nitrides having a maximum diameter of 50 to 1000 nm are contained in the number of 0.4/?m2 or larger in cross section observation.

Abstract: In accordance with certain embodiments, patterns are formed in composite materials by selectively urging one or more regions of the composite material toward an abrasion head, whereby the abrasion head mechanically removes portions of a patternable material from the composite material in each region.

Abstract: A method for isolating scandium values is provided. The method includes providing a scandium-bearing ore; subjecting the scandium-bearing ore to an acid leaching process, thereby obtaining a leachate, wherein the pH of the leachate is less than 1.

Abstract: Provided is a copper-base alloy with excellent wear resistance. The wear-resistant copper-base alloy includes, by mass %: 5.0 to 30.0% nickel; 0.5 to 5.0% silicon; 3.0 to 20.0% iron; less than 1.0% chromium; less than or equal to 5.0% niobium; less than or equal to 2.5% carbon; 3.0 to 20.0% of at least one element selected from the group consisting of molybdenum, tungsten, and vanadium; 0.5 to 5.0% manganese and/or 0.5 to 5.0% tin; balance copper; and inevitable impurities, and has a matrix and hard particles dispersed in the matrix, when niobium is contained, the hard particles contain niobium carbide and at least one compound selected from the group consisting of Nb—C—Mo, Nb—C—W, and Nb—C—V around the niobium carbide, and when niobium is not contained, the hard particles contain at least one compound selected from the group consisting of molybdenum carbide, tungsten carbide, and vanadium carbide.

Abstract: A high temperature, high strength Ni—Co—Cr alloy is provided. The alloy includes, in weight percent (wt. %): 23.5 to 25.5% Cr, 15.0 to 22.0% Co, 1.1 to 2.0% Al, 1.0 to 1.8% Ti, 0.95 to 2.2% Nb, less than 1.0% Mo, less than 1.0% Mn, up to 0.24% Si, less than 3.0% Fe, less than 0.3% Ta, less than 0.3% W, 0.005 to 0.08% C, 0.01 to 0.3% Zr, 0.0008 to 0.006% B, up to 0.05% rare earth metals, and a balance of Ni plus trace impurities.

Abstract: Disclosed is a magnesium alloy material having excellent tensile strength and favorable ductility. Therefore, the magnesium alloy sheet material formed by rolling a magnesium alloy having a long period stacking order phase crystallized at the time of casting includes in a case where a sheet-thickness traverse section of an alloy structure is observed at a substantially right angle to the longitudinal direction by a scanning electron microscope, a structure mainly composed of the long period stacking order phase, in which, at least two or more ?Mg phases having thickness in the observed section of 0.5 ?m or less are laminated in a layered manner with the sheet-shape long period stacking order phase.

Abstract: In an example of a method for forming a high-strength, lightweight alloy, starting materials are provided. The starting materials include aluminum, iron, and silicon. The starting materials are ball milled to generate the high-strength, lightweight alloy of a stable AlxFeySiz phase, wherein x ranges from about 3 to about 5, y ranges from about 1.5 to about 2.2, and z is about 1.

Abstract: In an embodiment, a magnetic body includes soft magnetic alloy grains 11 containing Fe, element L, and element M (where element L is Si or Zr and element M is a metal element other than Si or Zr that oxidizes more easily than Fe), as well as oxide film produced from oxidization of part of these grains 11; wherein at least some of the bonds between adjacent soft magnetic alloy grains 11 are by way of the oxide film; the oxide film has an inner film 12a, and an outer film 12b positioned on the outer side of the inner film 12a; and the inner film 12a contains more of element L than element M, while the outer film 12b contains more of element M than element L.

Abstract: There is provided a high-strength steel sheet having a tensile strength (TS) of 900 MPa or more and excellent elongation, stretch flangeability, and bendability in a component system in which an expensive alloy element is not intentionally added. A high-strength steel sheet includes a composition including, on a mass % basis, C: 0.15 to 0.40%, Si: 1.0 to 2.0%, Mn: 1.5 to 2.5%, P: 0.020% or less, S: 0.0040% or less, Al: 0.01 to 0.1%, N: 0.01% or less, and Ca: 0.0020% or less, with the balance including Fe and incidental impurities. The high-strength steel sheet has a microstructure including 40% to 70% of a ferrite phase and a bainite phase in total, 20% to 50% of a martensite phase, and 10% to 30% of a retained austenite phase in terms of an area fraction relative to the entire microstructure.

Abstract: A heat-resistant cold rolled ferritic stainless steel sheet containing, in mass %, 0.02% or less of C, 0.1% to 1.0% of Si, greater than 0.6% to 1.5% of Mn, 0.01% to 0.05% of P, 0.0001% to 0.0100% of S, 13.0% to 20.0% of Cr, 0.1% to 3.0% of Mo, 0.005% to 0.20% of Ti, 0.3% to 1.0% of Nb, 0.0002% to 0.0050% of B, 0.005% to 0.50% of Al, 0.02% or less of N, with the balance being Fe and inevitable impurities, in which {111}-oriented grains are present at an area ratio of 20% or greater in a region from a surface layer to t/4 (t is a sheet thickness), {111}-oriented grains are present at an area ratio of 40% or greater in a region from t/4 to t/2, and {011}-oriented grains are present at an area ratio of 15% or less in the entire region in a thickness direction.