Patents Issued in April 18, 2019
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Publication number: 20190112538Abstract: Disclosed is a solvent composition and use thereof for removal of one or more acid gas from a fluid stream comprising: i) a physical solvent, ii) an alkali metal organoborate, where R3 and R4 may be the same or different and are independently an alkylene group comprising 2 to 6 carbons, R5 and R6 may be the same or different and are independently H or a linear or branched alkyl group comprising from 1 to 18 carbons, and Z is an alkali metal ion; and iii) optionally an amine compound.Type: ApplicationFiled: March 16, 2017Publication date: April 18, 2019Inventor: Christophe Laroche
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Publication number: 20190112539Abstract: The composition of the present invention related to a quaternary ammonium salt detergent and the use of such quaternary ammonium salt detergents in a fuel composition to reduce diesel injector deposits and remove or clean up existing deposits on the diesel injectors.Type: ApplicationFiled: December 18, 2018Publication date: April 18, 2019Inventors: James H. Bush, Robert H. Barbour, David J. Moreton, Hannah Greenfield, Paul R. Stevenson, David C. Arters
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Publication number: 20190112540Abstract: A water based dispersion that includes lubricating nanoparticles. The lubricating nanoparticles can be provided by at least one intercalation nanoparticle of a metal chalcogenide in dispersion with the water base. The at least one intercalation nanoparticle may have a geometry that is fullerene-like, tubular-like structure or be substantially spherical or the intercalation nanoparticles may include particles have each of the aforementioned geometries. The intercalation nanoparticle is surface treated with a dispersant that is water soluble and includes a polar functional group.Type: ApplicationFiled: January 4, 2017Publication date: April 18, 2019Inventors: George Diloyan, Girija S. Chaubey, Debapriya Das
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Publication number: 20190112541Abstract: Provided are a lubricating oil composition containing a synthetic oil (1) having a kinematic viscosity at 100° C. of 2.5 mm2/s or less, and a polymethacrylate (2) having a monovalent functional group containing an oxygen atom in the molecule while having a specific structural unit, which satisfies both high viscosity index and high shear stability, and a lubrication method and a transmission using the lubricating oil composition.Type: ApplicationFiled: March 2, 2017Publication date: April 18, 2019Applicant: IDEMITSU KOSAN CO., LTD.Inventor: Tatsuya HAMACHI
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Publication number: 20190112542Abstract: A lubricating composition includes an oil of lubricating viscosity and an oil-soluble dispersant viscosity modifier. The dispersant viscosity modifier includes an olefin-based polymer backbone and at least one pendent group derived from a functionalized aliphatic amine. Each pendent group is independently attached to the olefin-based polymer backbone. The pendent group is non-ionic, non-basic, and non-acidic and includes a functional group which includes at least one heteroatom other than nitrogen.Type: ApplicationFiled: December 10, 2018Publication date: April 18, 2019Applicant: The Lubrizol CorporationInventors: ADAM PRESTON, Yanshi Zhang, Sona S. Slocum, Matthew .D. Gieselman
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Publication number: 20190112543Abstract: An aircraft hydraulic system utilizes a hydraulic fluid that includes a heat transfer fluid and a friction modifier additive.Type: ApplicationFiled: October 13, 2017Publication date: April 18, 2019Inventor: Marc E. Gage
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Publication number: 20190112544Abstract: The present invention relates to a lubricating oil composition containing: a base oil, (A) a benzotriazole derivative having a particular structure, (B) an epoxy compound, and (C) an ashless friction modifier, the ashless friction modifier (C) being contained in an amount of 0.20% by mass or more based on the total amount of the lubricating oil composition.Type: ApplicationFiled: March 27, 2017Publication date: April 18, 2019Applicant: IDEMITSU KOSAN CO., LTD.Inventor: Nao YOSHIMURA
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Publication number: 20190112545Abstract: A lubricating oil composition which comprises a sulphur containing anti-oxidant and an alkene which exhibits improved nitrile elastomer seal compatibility.Type: ApplicationFiled: October 12, 2018Publication date: April 18, 2019Applicant: Infineum International LimitedInventors: Oliver J. Delamore, Nigel A. Male, Alastair A. Cant, Philip J. Woodward
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Publication number: 20190112546Abstract: The present invention relates to a lubricated system comprising: a) a component comprising a first surface which is a diamond-like carbon (DLC) surface; b) a component comprising a second surface; and c) a lubricant formulation interposed between the first surface and second surface. The lubricant formulation comprises: i) a base stock; ii) a molybdenum compound; iii) an organic polymer comprising a hydrophobic polymeric sub unit selected from polyesters and functionalised polyolefins and a hydrophilic polymeric sub unit selected from polyethers; and iv) optionally, other additives. The combination of the molybdenum compound and the organic polymer in the lubricant formulation reduces the wear on one or more of the surfaces in the lubricated system when compared with using the molybdenum containing friction modifier alone.Type: ApplicationFiled: March 14, 2017Publication date: April 18, 2019Applicant: Croda International PlcInventors: Gareth Moody, John Eastwood, Aitziber Viadas Cienfuegos
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Publication number: 20190112547Abstract: A hybrid mint plant characterized by an essential oil composition profile, methods of cultivating the hybrid mint plant, and methods of producing an essential oil composition with the essential oil composition profile using the hybrid mint plant are disclosed.Type: ApplicationFiled: May 15, 2018Publication date: April 18, 2019Inventor: Donald D. Roberts
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Publication number: 20190112548Abstract: Highly concentrated liquid detergents with a surfactant content of 30 wt. % and more, also containing polymers in concentrations of 2 wt. % or more and displaying optimised product stability. The detergent having a specifically adapted surfactant system containing alkyl ether alkoxylates, alkyl(poly)gylcosides and additional surfactants. The invention also relates to methods for washing textiles using the described detergents and to the use thereof.Type: ApplicationFiled: December 13, 2018Publication date: April 18, 2019Applicant: Henkel AG & Co. KGaAInventors: Ulrich Pegelow, Sabine Schuemann, Simon Pluszynski, Anna Klemmer, Thorsten Bastigkeit
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Publication number: 20190112549Abstract: A detergent composition comprises a trisiloxane having at least one carbinol functional group. The trisiloxane comprises the reaction product of (A) an initial trisiloxane and (B) an organic compound. Component (A) has a pendant silicon-bonded functional group selected from a hydrogen atom, an epoxy-containing group, an ethylenically unsaturated group, and an amine group. Typically, component (A) is free of certain terminal silicon-bonded functional groups and is free of polyoxyalkylene groups. Component (B) has a functional group reactive with the pendant silicon-bonded functional group of component (A), and has at least one hydroxyl functional group. The trisiloxane may be of the following general formula (I): (R13SiO1/2)(R1R3SiO2/2)(R13SiO1/2) (I). In formula (I), each R1 is an independently selected hydrocarbyl group. R3 may be selected from the groups (i) to (x) described herein, each of the groups having at least one carbinol group. Typically, the trisiloxane has 1-6 carbinol groups.Type: ApplicationFiled: April 26, 2017Publication date: April 18, 2019Inventors: Paula Michele BAUER, Severine CAUVIN, Eric J. JOFFRE, Lenin J. PETROFF, Martin K. SEVERANCE, Tsunehito SUGIURA, Seiki TAMURA, Zachary R. WENZLICK
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Publication number: 20190112550Abstract: A laundry care composition including (a) at least one laundry care ingredient and (b) a leuco composition. The leuco composition has a first color state and a second color state and the mole ratio of second color state to first color state is from 2:98 to 5:95. Also disclosed are methods of determining the approximate functional age of a laundry care composition comprising the steps of (a) providing a laundry care composition that includes at least one laundry care ingredient and a leuco composition, (b) providing a functional age scale that includes a plurality of distinct colors that correspond to a unique functional age and (c) comparing the color of the laundry care composition with the functional age scale.Type: ApplicationFiled: October 11, 2018Publication date: April 18, 2019Inventors: Gregory Scot MIRACLE, Daniel Dale DITULLIO, JR., Wesley A. FREUND, Haihu QIN, Sanjeev Kumar DEY
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Publication number: 20190112551Abstract: The present disclosure provides a laundry care composition having at least one laundry care ingredient and at least one leuco composition conforming to Formula (I): Ar1Ar2Ar3CH??(I) wherein Ar2 and Ar3 are independently a carbocyclic aryl or heteroaryl, and Ar1 is selected from the group consisting of: unsubstituted phenyl, electron deficient carbocyclic aryl, and heteroaryl. The present disclosure also provides a method of determining an authentic laundry care composition and test kits for detecting authentic laundry care compositions.Type: ApplicationFiled: October 11, 2018Publication date: April 18, 2019Inventors: Gregory Scot MIRACLE, Daniel Dale DITULLIO, JR., Sanjeev Kumar DEY, Haihu QIN
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Publication number: 20190112552Abstract: The present disclosure relates to proteases having an amino acid sequence with at least 70% sequence identity to the amino acid sequence given in SEQ ID No. 1, across its whole length, and comprising an amino acid substitution on at least one of the positions P9, Q10, Q62, L82, P86, N130, T141, N187, S236 or T253, relating in each case to the numbering according to SEQ ID No. 1. The present disclosure also relates to the production and use thereof. Said type of proteases have a very good cleaning performance.Type: ApplicationFiled: March 7, 2017Publication date: April 18, 2019Applicant: Henkel AG & Co. KGaAInventors: Nina Mussmann, Timothy O'Connell, Daniela Herbst, Inken Prueser, Christian Degering, Sabine Griemert, Thorsten Eggert, Christian Leggewie
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Publication number: 20190112553Abstract: A disinfecting solution includes hydrogen peroxide at a concentration of from about 1.5% w/w to 8% w/w; at least one readily biodegradable anionic surfactant in a concentration of from about 10% w/w to about 25% w/w; at least one of salicylic acid and salts thereof, in a concentration of from about 1% w/w to about 8% w/w; at least one chelating agent in a concentration of from about 0.005% w/w to about 10% w/w; and at least one low-corrosive non-surfactant sulfonic acid selected from benzenesulfonic acid, benzene disulfonic acid, xylene sulfonic acid, toluene sulfonic acid, cumene sulfonic acid, sulfosalicylic acid, naphthalene mono- or di-sulfonic acid, methane sulfonic acid, and isethionic acid in a concentration of from about 1% w/w to about 15% w/w. The solution is free of acetic acid, furoic acid, phenylacetic acid, cationic surfactants, amphoteric surfactants, aromatic alcohols, halogen acids, phenols, peracid activators, bleaches, and (optionally) benzoic acid.Type: ApplicationFiled: October 18, 2017Publication date: April 18, 2019Inventor: Randall PILON
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Publication number: 20190112554Abstract: A method for treating textile articles including the steps of: (a) providing a laundry care composition comprising a leuco composition; (b) adding the laundry care composition to a liquid medium; (c) placing the textile articles in contact with the liquid medium; (d) depositing at least some portion of the leuco composition onto the textile articles; (e) optionally, rinsing the textile; and (f) drying the textile articles, wherein the leuco composition comprises a leuco colorant with a Photoresistance Index of less than or equal to 30.Type: ApplicationFiled: October 11, 2018Publication date: April 18, 2019Inventors: Gregory Scot MIRACLE, Daniel Dale DITULLIO, JR., Wesley A. FREUND, Haihu QIN, Sanjeev Kumar DEY
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Publication number: 20190112555Abstract: A laundry care composition including (a) at least one laundry care ingredient and (b) at least one fluorescent moiety covalently bound to at least one second moiety selected from the group consisting of leuco moieties and chromophore moieties. A method of treating a textile includes the steps of (a) providing such a laundry care composition; (b) adding the laundry care composition to a liquid medium; (c) placing textile articles in the liquid medium; (d) optionally, rinsing the textile; and (e) drying the textile articles.Type: ApplicationFiled: October 11, 2018Publication date: April 18, 2019Inventors: Gregory Scot MIRACLE, Dominick Joseph VALENTI, Sanjeev Kumar DEY, Haihu QIN, Wesley A. FREUND
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Publication number: 20190112556Abstract: A laundry care composition including laundry care ingredient and a plurality of whitening agents.Type: ApplicationFiled: October 11, 2018Publication date: April 18, 2019Inventors: Gregory Scot MIRACLE, Sanjeev Kumar DEY, Haihu QIN
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Publication number: 20190112557Abstract: A laundry care composition including (a) at least one laundry care ingredient and (b) a compound of Formula (XL) A method of treating a textile comprises the steps of (a) providing such a laundry care composition; (b) adding the laundry care composition to a liquid medium; (c) placing textile articles in the liquid medium; (d) optionally, rinsing the textile; and (e) drying the textile articles.Type: ApplicationFiled: October 11, 2018Publication date: April 18, 2019Inventors: Gregory Scot MIRACLE, Sanjeev Kumar DEY, Wesley A. FREUND
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Publication number: 20190112558Abstract: A laundry care composition including (a) at least one laundry care ingredient and (b) a leuco composition including at least one leuco compound. The leuco compound including a leuco moiety and a polyalkyleneoxy moiety covalently bound to the leuco moiety. The polyalkyleneoxy moiety includes at least one ethylene oxide group and at least one propylene oxide group. A method of treating a textile includes the steps of (a) providing such a laundry care composition; (b) adding the laundry care composition to a liquid medium; (c) placing textile articles in the liquid medium; (d) optionally, rinsing the textile; and (e) drying the textile articles.Type: ApplicationFiled: October 11, 2018Publication date: April 18, 2019Inventors: Gregory Scot MIRACLE, Daniel Dale DITULLIO, JR., Haihu QIN, Sanjeev Kumar DEY, Wesley A. FREUND, Randy D. PETREA
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Publication number: 20190112559Abstract: A method for pre-treating textile articles includes the steps of: (a) providing a laundry care composition comprising a laundry care ingredient and a leuco composition comprising at least 0.001 wt % leuco colorant, based on the weight of the laundry care composition; (b) placing the laundry care composition in contact with a stain on the textile article in a pretreat step; (c) placing the textile articles in contact with a liquid medium optionally further comprising a converting agent; (d) optionally converting at least some portion of the leuco composition to form an oxidized leuco composition; (e) depositing at least a portion of the leuco composition and/or the oxidized leuco composition onto the textile; (f) optionally, rinsing the textile; and (g) drying the textile articles.Type: ApplicationFiled: October 11, 2018Publication date: April 18, 2019Inventors: Gregory Scot MIRACLE, Daniel Dale DITULLIO, JR., Wesley A. FREUND, Diana C. BALASCA, Sanjeev Kumar DEY
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Publication number: 20190112560Abstract: A laundry care composition including (a) at least one laundry care ingredient and (b) a leuco compound including an antioxidant moiety covalently bonded to a leuco moiety.Type: ApplicationFiled: October 11, 2018Publication date: April 18, 2019Inventors: Gregory Scot MIRACLE, Sanjeev Kumar DEY, Haihu QIN, Dominick Joseph VALENTI
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Publication number: 20190112561Abstract: A laundry care composition including at least one laundry care ingredient, a leuco composition, from 0.05 wt % to 15 wt % of a primary amine compound and from 0.005 wt % to 5 wt % of an antioxidant.Type: ApplicationFiled: October 11, 2018Publication date: April 18, 2019Inventors: Gregory Scot MIRACLE, Brian Joseph LOUGHNANE, Samantha Jo COST, Wesley A. FREUND, Haihu QIN, Sanjeev Kumar DEY
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Publication number: 20190112562Abstract: The present disclosure relates to a composition for cleaning or sanitizing a surface, methods of using the same for cleaning and sanitizing a surface, and kits comprising the same. The composition includes an enzyme isolated from a hyperthermophilic and/or acidophilic organism and optionally, an acid and an additive. The composition can be used at temperatures ranging from about 50° C. to 110° C., preferably at temperatures ranging from about 70° C. to 100° C. In addition, the composition can be used at pH values ranging from 0.5 to 7, preferably at pH values ranging from 2 to 5.Type: ApplicationFiled: April 6, 2017Publication date: April 18, 2019Applicant: Cinder Biological, Inc.Inventors: Steven M. Yannone, Jill O. Fuss, Adam Barnebey
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Publication number: 20190112563Abstract: Provided herein are detergent formulations which can effectively suppress efflorescence in unit dose detergent packs. In accordance with one embodiment, the detergent formulations comprise an alkyl ethoxysulfate component having an alkyl chain length of about 12-18 carbon atoms and about 2-9 moles of ethylene oxide and a cation, an alcohol ethoxylate component having an alkyl chain length of about 12-18 carbon atoms and about 5-9 moles of ethylene oxide, high fructose corn syrup, and a liquid carrier.Type: ApplicationFiled: October 12, 2017Publication date: April 18, 2019Applicant: Henkel IP & Holding GmbHInventor: Daniel Thomas Piorkowski
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Publication number: 20190112564Abstract: The present invention relates to a ready-to-drink alcoholic beverage comprising a distilled spirit and at least one Aloaceae source.Type: ApplicationFiled: October 12, 2018Publication date: April 18, 2019Inventor: Keith Cyzen
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Publication number: 20190112565Abstract: The present disclosure provides a membrane separation method of a cell suspension which can appropriately separate cells from debris, and a cell culture device. That is, membrane separation processing of the cell suspension is performed using a filtration membrane which includes an inlet-side opening formed on one surface and an outlet-side opening, which is formed on the other surface and communicates with the inlet-side opening, and in which the inlet-side opening and the outlet-side opening are disposed at positions deviated in a direction parallel to the surfaces of the membrane.Type: ApplicationFiled: December 13, 2018Publication date: April 18, 2019Inventors: Hideaki KAGAWA, Yoichi NAGAI, Shinichi NAKAI, Souichi KOHASHI, Toshiki TAKEI
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Publication number: 20190112566Abstract: A perfusion manifold assembly is described that allows for perfusion of a microfluidic device, such as an organ on a chip microfluidic device comprising cells that mimic cells in an organ in the body, that is detachably linked with said assembly so that fluid enters ports of the microfluidic device from a fluid reservoir, optionally without tubing, at a controllable flow rate. A culture module is contemplated that allows the perfusion and optionally mechanical actuation of one or more microfluidic devices, such as organ-on-a-chip microfluidic devices comprising cells that mimic at least one function of an organ in the body.Type: ApplicationFiled: December 17, 2018Publication date: April 18, 2019Inventors: Christopher David Hinojosa, Guy Robert Thompson, II, Joshua Gomes, Jacob Freake, Doug Sabin
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Publication number: 20190112567Abstract: A vessel for culturing cells is provided that includes a vessel body having a top portion, a bottom portion comprising a bottom interior surface, and a cylindrical sidewall. The vessel additionally has an impeller assembly inside the vessel body having a top portion rotatably coupled to the top portion of the vessel body, the impeller assembly having a plurality of planar blades, a central axis, a flexible shaft extending down from the top portion of the impeller assembly, a magnet receptacle molded within the plurality of planar blades, a magnet within the magnet receptacle, and an impeller o-ring coupled to a bottom surface of the planar blades. The vessel also includes a plurality of positioning nubs coupled to the bottom interior surface of the vessel body, spaced from an inside edge of the impeller o-ring.Type: ApplicationFiled: May 31, 2017Publication date: April 18, 2019Inventors: Jeffery Joseph Scibek, James Clark Walck, JR.
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Publication number: 20190112568Abstract: The present invention relates to methods and systems of pitching yeast to fermentation reactors. More particularly, the present invention involves pitching yeast from one fermentation tank to at least one additional fermentation tank. Advantageously, yeast can be continuously pitched from fermentor to fermentor for as long as practically desirable.Type: ApplicationFiled: December 13, 2018Publication date: April 18, 2019Inventors: Neelakantam V. Narendranath, Stephen M. Lewis, Neil D. Anderson
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Publication number: 20190112569Abstract: The present invention provides in situ Raman spectroscopy methods and systems for monitoring and controlling one or more process variables in a bioreactor cell culture in order to improve product quality and consistency. The methods and systems utilize in situ Raman spectroscopy and chemometric modeling techniques for real-time assessments of cell cultures, combined with signal processing techniques, for precise continuous feedback and model predictive control of cell culture process variables. Through the use of real-time data from Raman spectroscopy, the process variables within the cell culture may be continuously or intermittently monitored and automated feedback controllers maintain the process variables at predetermined set points or maintain a specific feeding protocol that delivers variable amounts of agents to the bioreactor to maximize bioproduct quality.Type: ApplicationFiled: October 15, 2018Publication date: April 18, 2019Inventors: Mark Czeterko, Anthony DeBaise, William Pierce, Matthew Conway
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Publication number: 20190112570Abstract: Cell separation systems and methods of separating cells are disclosed. In an embodiment, a cell separation system is described that comprises a non-transitory storage device that executes a centrifugation program to separate cell volume from biologic material volume; a heating mechanism; a containment mechanism; and an assembly comprised of a single-walled centrifugation bowl. In an embodiment, methods of separating cells are disclosed whereby cells are separated by agitating a volume of biologic material and a volume digestion media to form a digested volume of biologic material; centrifuging the digested volume of biologic material; removing a portion of a resulting waste via at least one fluid outlet; isolating a different portion of the waste, and removing the concentrated cell volumes from the reservoir.Type: ApplicationFiled: December 13, 2018Publication date: April 18, 2019Applicant: TISSUE GENESIS, LLCInventors: Rolf WOLTERS, Jules VALENTI, Tom GURSKI
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Publication number: 20190112571Abstract: The use of cell matter in fermentation mixtures for producing a product is disclosed. In embodiments, the product comprises carbohydrates, alcohols, or organic acids (e.g., lactic acid or succinic acid), or mixtures thereof.Type: ApplicationFiled: March 30, 2016Publication date: April 18, 2019Inventors: Marshall Medoff, Thomas Craig Masterman, Michael W. Finn
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Publication number: 20190112572Abstract: A cell culture medium comprising tyrosine at a concentration of at least 3 mM and polyvinylalcohol (PVA).Type: ApplicationFiled: March 23, 2017Publication date: April 18, 2019Applicant: Pfizer Inc.Inventors: Bruno FIGUEROA, Yen-Tung Luan, Wenge Wang
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Publication number: 20190112573Abstract: A method of differentiating pluripotent stem cells into mesenchymal stem cells, a culture medium used in the method of differentiating pluripotent stem cells into mesenchymal stem cells and a method of performing tissue and organ regeneration by using the mesenchymal stem cells obtained by differentiation using the method of differentiating pluripotent stem cells into mesenchymal stem cells are provided. The method of differentiating pluripotent stem cells into mesenchymal stem cells comprises differentiating, completely under 3D suspension conditions, pluripotent stem cells into trophoblast-like cells using BMP4 and A8301, and then differentiating the trophoblast-like cells into mesenchymal stem cells. Neither of two differentiation processes needs passaging or replacement of a culture container.Type: ApplicationFiled: July 20, 2018Publication date: April 18, 2019Applicant: University of MacauInventors: Ren-He Xu, Bin Jiang, Li Yan
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Publication number: 20190112574Abstract: A culture method for enhancing the activity of stem cells, and a method of preparing a formulation for treating central nervous diseases, and a method of treating central nervous diseases are provided. In some aspects, stem cell clusters are cultured for 3 to 11 days in a sealed container filled with a culture medium, under a condition in which no oxygen and carbon dioxide are supplied. By placing stem cells in a sealed container to stabilize the properties and enhance the functions thereof under a room temperature condition, the stemness and apoptosis resistance of the stem cells are improved, thereby significantly improving the therapeutic effect of the stem cells after transplantation into a human body.Type: ApplicationFiled: July 27, 2018Publication date: April 18, 2019Inventors: Ren-He Xu, Bin Jiang, Li Yan, Enqin Li
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Publication number: 20190112575Abstract: A cell population comprising Corin- and/or Lrtm1-positive cells was produced by the following steps (1) and (2), from which Corin positive and/or Lrtm1 positive cells are collected using a substance that binds to Corin and/or a substance that binds to Lrtm1, and dopaminergic neuron progenitor cells are produced by performing suspension culture of the Corin positive and/or Lrtm1 positive cells in a culture solution containing one or more nutritional factors: (1) a step of performing adhesion culture of pluripotent stem cells in a medium for maintaining undifferentiated state containing a Sonic hedgehog (SHH) signal stimulant, and an undifferentiated state-maintaining factor in the absence of feeder cells but in the presence of an extracellular matrix, and (2) a step of culturing the cell population obtained in the step (1) in a culture solution containing one or more differentiation-inducing factors.Type: ApplicationFiled: April 24, 2017Publication date: April 18, 2019Applicants: KYOTO UNIVERSITY, SUMITOMO DAINIPPON PHARMA CO., LTD.Inventors: Jun TAKAHASHI, Daisuke DOI, Kenji YOSHIDA, Atsushi KUWAHARA, Masayo TAKAHASHI
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Publication number: 20190112576Abstract: Provided herein are methods for culturing cells, including stimulating or expanding (proliferating), a plurality of cells in a composition of cells such as a population of lymphocytes. In some aspects, provided methods and reagents for the culturing, such as stimulation or expansion (proliferation), of cell populations involve binding of agents to a molecule on the surface of the cells, thereby providing one or more signals to the cells. In some cases, the reagents are multimerization reagents and the one or more agents are multimerized by reversibly binding to the reagent. In some aspects, the multimerized agent can provide for expansion or proliferation or other stimulation of a population of cells, and then such stimulatory agents can be removed by disruption of the reversible bond. Also provided are compositions, apparatus and methods of use thereof.Type: ApplicationFiled: October 20, 2016Publication date: April 18, 2019Applicant: Juno Therapeutics GmbHInventors: Lothar GERMEROTH, Christian STEMBERGER, Patricia GRÄF
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Publication number: 20190112577Abstract: Provides an ex vivo expansion method for a cord blood NK cell, and a kit and application thereof. The ex vivo expansion method for the cord blood NK cell comprises: separating a cord blood monocyte; activating, culturing, and proliferation; and culturing of the cord blood NK cell. The invention does not require cell sorting and does not require a trophoblast. A reagent in the kit does not contain animal-based component. The invention provides a high production volume of the NK cell. The cord blood NK cell obtained has at least 90% purity, a total cell count of up to 1010-11 per part of cord blood, and can kill a tumor cell effectively.Type: ApplicationFiled: May 6, 2016Publication date: April 18, 2019Inventors: Xiaoyun WU, Xuemin LIU, Huiyan KANG, Yunhong WANG, Xiaoxiang FAN, Zhigang LI, Wei DING, Lihua WU, Jinyan LI, Dianfu SHEN, Huizhen ZHANG
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Publication number: 20190112578Abstract: The present invention pertains inter alia to novel efficient methods for culturing mesenchymal stem (stromal) cells (MSCs), wherein the MSCs have maintained and/or enhanced multipotency and/or maintain and/or enhanced immunomodulatory potential. The invention also pertains to cells obtainable by such methods, kits and compositions for carrying out the methods in accordance with the invention, and also medical applications and treatments using said cells.Type: ApplicationFiled: April 26, 2016Publication date: April 18, 2019Inventors: Oscar Simonson, Karl-Henrik Grinnemo
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Publication number: 20190112579Abstract: The current invention provides for methods of promoting differentiation of human pluripotent stem cells into esophageal progenitor cells as well as the cells obtained from the methods, solutions, compositions, and pharmaceutical compositions comprising such cells. The current invention also provides for methods of using the esophageal progenitor cells for treatment and prevention of disease, and kits.Type: ApplicationFiled: October 10, 2018Publication date: April 18, 2019Inventors: Jianwen Que, Yongchun Zhang
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Publication number: 20190112580Abstract: This disclosure relates to the use of DNA methyltransferase inhibitors, alone or in combination with Aurora kinase inhibitors, to treat muscle cells isolated from contractured muscles or to prepare isolated and treated muscle cell compositions. The analogs or drug combinations or cell compositions can be used to treat or to prevent skeletal muscle contractures, skeletal muscle atrophy, and muscle tissue remodeling in patients such as those with cerebral palsy. A method is provided for the treatment of the conditions listed above using muscle cells treated with DNA methyltransferase inhibitors in vitro, alone or along with other active agents, cells or biocompatible materials, and transplanted into patients with cerebral palsy or other muscle conditions.Type: ApplicationFiled: October 16, 2018Publication date: April 18, 2019Applicant: Rehabilitation Institute of Chicago d/b/a Shirley Ryan AbilityLabInventors: Andrea Alberto DOMENIGHETTI, Richard L. LIEBER
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Publication number: 20190112581Abstract: The present invention is directed generally to host cells with artificial endosymbionts, wherein the artificial endosymbiont and the host cell communicate with each other to alter a phenotype of the host cell. In some embodiments, the communication comprises the secretion of a polypeptide from the artificial endosymbiont into the host cell. The secreted polypeptide can be a selectable marker, a reporter protein, a transcription factor, a signal pathway protein, a receptor, a growth factor, a cytokine, an effector molecule or other factors that can produce a phenotype in the host cell.Type: ApplicationFiled: December 19, 2018Publication date: April 18, 2019Applicant: BELL BIOSYSTEMS, INC.Inventors: Caleb B. Bell, III, Alexey Bazarov, Abdul Wakeel, Joyce Barrozo
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Publication number: 20190112582Abstract: The present invention refers to an In vitro method for screening for subjects at risk of developing thoracic aortic aneurysm (TAA) or a disease causing TAA comprising: (a) measuring the expression pattern or level of at least A Disintegrin And Metalloproteinase with Thrombospondin Motifs 1 (ADAMTS1) obtained from an isolated biological sample of the subjects to be screened; and (b) comparing said expression pattern or level of at least ADAMTS1 of the subjects to be screened with an already established expression pattern or level, wherein reduced expression of at least ADAMTS1 is indicative of a thoracic aortic aneurysm (TAA).Type: ApplicationFiled: December 30, 2016Publication date: April 18, 2019Inventors: Juan Miguel REDONDO MOYA, Nerea MÉNDEZ-BARBERO, Jorge OLLER PEDROSA, Miguel Ramón CAMPANERO GARCÍA
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Publication number: 20190112583Abstract: The present invention is directed to all aspects of novel methyl transferase enzymes that methylate backbone amides of (poly)peptides. The present invention also relates to nucleic acids encoding these enzymes as well as corresponding vectors and host cells comprising these. Moreover, the present invention encompasses the use of said enzymes for modifying (poly)peptides as well as corresponding methods. Also, the present invention pertains to further novel enzymes for modifying (poly)peptides derived from the omphalotin gene cluster of O. olearius and the homologous gene clusters from D. bispora, L. edodes and F. mediterranea as well as related aspects.Type: ApplicationFiled: April 7, 2017Publication date: April 18, 2019Inventors: Markus AEBI, Markus KUNZLER, Jorn PIEL, Michael FREEMAN, Niels VAN DER VELDEN, Noemi KALIN
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Publication number: 20190112584Abstract: In one aspect, the invention relates to an immunogenic composition that includes a mutant Clostridium difficile toxin A and/or a mutant Clostridium difficile toxin B. The mutant toxin may include a glucosyltransferase domain having at least one mutation and a cysteine protease domain having at least one mutation, relative to the corresponding wild-type C. difficile toxin. The mutant toxins may include at least one amino acid that is chemically crosslinked. In another aspect, the invention relates to methods and compositions for use in culturing Clostridium difficile and in producing C. difficile toxins.Type: ApplicationFiled: August 19, 2018Publication date: April 18, 2019Inventors: Jason Arnold Lotvin, Annaliesa Sybil Anderson, Robert G. K. Donald, Michael James Flint, Narender Kumar Kalyan, Kathrin Ute Jansen, Maninder K. Sidhu, Justin Keith Moran, Mark Edward Ruppen, Weiqiang Sun
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Publication number: 20190112585Abstract: Reactions comprising water, sucrose, and one or more glucosyltransferase enzymes are disclosed herein. Glucosyltransferase enzymes used in these reactions comprise certain motifs allowing production of insoluble poly alpha-1,3-glucan having at least 95% alpha-1,3 glycosidic linkages.Type: ApplicationFiled: November 14, 2018Publication date: April 18, 2019Inventors: Mark S. Payne, Yefim Brun, Richard R. Bott
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Publication number: 20190112586Abstract: Aspects of the invention provide engineered endonucleases that are characterized by both a long recognition sequence and specific cleavage outside of the recognition site. Engineered endonucleases of the invention are useful for manipulating long pieces of DNA.Type: ApplicationFiled: May 17, 2018Publication date: April 18, 2019Inventors: Shaun M. Lippow, Dasa Lipovsek, Patricia M. Aha
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Publication number: 20190112587Abstract: The present disclosure provides engineered cross-type-nucleic-acid targeting nucleic acids and compositions thereof. Nucleic acid sequences encoding the engineered cross-type-nucleic-acid targeting nucleic acids, as well as expression cassettes, vectors and cells comprising such nucleic acid sequences, are described. Also, methods are disclosed for making and using the engineered cross-type-nucleic-acid targeting nucleic acids and compositions thereof.Type: ApplicationFiled: December 18, 2018Publication date: April 18, 2019Inventors: Paul Daniel Donohoue, Andrew Paul May