Abstract: Described herein are cell-based analytic methods, including a method of incorporating nucleic acid sequences into reaction products from a cell population, wherein the nucleic acid sequences are incorporated into the reaction products of each cell individually or in small groups of cells individually. Also described herein is a matrix-type microfluidic device that permits at least two reagents to be delivered separately to each cell or group of cells, as well as primer combinations useful in the method and device.

Abstract: Methods for preparing sequencing libraries from a DNA-containing test sample, as well as methods for correcting sequencing-derived errors in sequence reads, and methods for identifying rare variants in a test sample, are provided.

Abstract: Aspects of the present invention include analyzing nucleic acids from single cells using methods that include using tagged polynucleotides containing multiplex identifier sequences.

Abstract: Provided is a method for preparing libraries for massively parallel sequencing. The method includes: providing two or more double-stranded nucleic acid molecules; ligating adaptors to both ends of each of the nucleic acid molecules; providing a pair of primers for amplifying each nucleic acid molecule wherein each of the paired primers includes i) a 3?-end having a nucleotide sequence complementary to the corresponding adaptor, ii) a 5?-end having a common primer sequence for massively parallel sequencing, and iii) an index sequence located between the 3?-end and the 5?-end, one of the two index sequences is a sequence specific to the corresponding nucleic acid molecule, and the other index sequence is a sequence indexing a sample from which the nucleic acid molecule is derived; and performing amplification using the paired primers to obtain amplification products of the nucleic acid molecules including the molecule-specific sequences and the sample indexing sequences.

Abstract: This disclosure describes methods for enabling accurate detection and quantitation of rare alleles within a DNA sample using low-depth sequencing, through the use of allele-specific enrichment and/or depletion hybridization probes. For example, methods are provided for using competitive probes to apply allele-specific enrichment or depletion to amplicons from multiplex PCR on a biological DNA sample.

Abstract: The present invention relates to novel nucleic acid molecules, called aptamers, that bind specifically to a small molecule fluorophore and thereby enhance the fluorescence signal of the fluorophore upon exposure to radiation of suitable wavelength. Molecular complexes formed between the novel fluorophores, novel nucleic acid molecules, and their target molecules are described, and the use of multivalent aptamer constructs as fluorescent sensors for target molecules of interest are also described.

Abstract: The invention provides methods and compositions for treating various degenerative diseases (e.g., AMD) with a factor D inhibitor (e.g., anti-factor D antibody or antigen-binding fragment thereof). Also provided are methods of selecting or identifying patients for treatment with a factor D inhibitor. Methods include the use of prognostic and/or predictive biomarkers.

Abstract: The invention provides methods for simultaneously amplifying multiple nucleic acid regions of interest in one reaction volume as well as methods for selecting a library of primers for use in such amplification methods. The invention also provides library of primers with desirable characteristics, such as minimal formation of amplified primer dimers or other non-target amplicons.

Abstract: The present invention is drawn to a method for in vitro predicting the risk for a patient to present a pathology or to identify whether a patient is at risk to develop a pathology, such as psychiatric disorder, associated to an alteration of A-to-I editing on PDE8A transcripts, from body fluids such as a blood, urine or saliva sample of said patient. The present invention also relates to kits for the implementation of the method.

Abstract: A method for determining the epigenetic age acceleration of an individual comprising measuring a methylation level of a set of methylation markers in genomic DNA of an individual. An epigenetic age of the individual is determined based on the measured methylation level. An epigenetic age of the individual is further determined based on a methylation derived weighted average cell count of naive cytotoxic T cells and exhausted cytotoxic T cells in the individual. The determined epigenetic age is then compared to a chronological age of the individual to determine an epigenetic age acceleration of the individual. Instances wherein the epigenetic age is greater than the chronological age of the individual is an indication of an increased risk of all-cause mortality.

Abstract: This document provides methods and materials involved in assessing samples (e.g., cancer cells) for the presence of homologous recombination deficiency (HRD) or an HRD signature. For example, methods and materials for determining whether or not a cell (e.g., a cancer cell) contains an HRD signature are provided. Materials and methods for identifying cells (e.g., cancer cells) having a deficiency in homology directed repair (HDR) as well as materials and methods for identifying cancer patients likely to respond to a particular cancer treatment regimen also are provided.

Abstract: The present disclosure provides a system and method for the detection of rare mutations and copy number variations in cell free polynucleotides. Generally, the systems and methods comprise sample preparation, or the extraction and isolation of cell free polynucleotide sequences from a bodily fluid; subsequent sequencing of cell free polynucleotides by techniques known in the art; and application of bioinformatics tools to detect rare mutations and copy number variations as compared to a reference. The systems and methods also may contain a database or collection of different rare mutations or copy number variation profiles of different diseases, to be used as additional references in aiding detection of rare mutations, copy number variation profiling or general genetic profiling of a disease.

Abstract: The present disclosure provides a system and method for the detection of rare mutations and copy number variations in cell free polynucleotides. Generally, the systems and methods comprise sample preparation, or the extraction and isolation of cell free polynucleotide sequences from a bodily fluid; subsequent sequencing of cell free polynucleotides by techniques known in the art; and application of bioinformatics tools to detect rare mutations and copy number variations as compared to a reference. The systems and methods also may contain a database or collection of different rare mutations or copy number variation profiles of different diseases, to be used as additional references in aiding detection of rare mutations, copy number variation profiling or general genetic profiling of a disease.

Abstract: The invention provides PIK3CA (phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit alpha) gene fusions, and fragments of those gene fusions. The invention further provides methods of diagnosing and treating diseases or disorders associated with PIK3CA fusions, such as conditions mediated by PIK3CA aberrant expression or activity, or overexpression of PIK3CA.

Abstract: The present disclosure provides methods for detecting a high-risk phenotype of a mast cell tumor (MCT) in a biological sample from a mammal, preferably a dog. Kits, PCR probes and primers to detect high-risk MCT are also provided.

Abstract: The current disclosure relates to therapeutic treatments and diagnostic methods for esophageal cancer based on the expression level of biomarker miRNAs.

Abstract: Provided herein are methods for detecting a head and neck cancer of the oral cavity or throat, optionally oral squamous cell carcinoma, comprising executing the step of determining the expression of two or more miRNA in a biological sample obtained from a subject, wherein the two or more miRNA are selected from the group consisting of hsa-let-7a, hsa-miR-16, hsa-miR-21, hsa-miR-451, hsa-miR-486-5p and hsa-miR-92a-3p, and wherein the level of expression of said two or more miRNAs in the biological sample relative to the level of expression of said two or more miRNAs in one or more cancer-free reference samples is indicative of the presence of a head and neck cancer of the oral cavity or throat of the subject.

Abstract: The present disclosure provides methods and kits for characterizing Cannabis plants. Methods and kits of the present disclosure include detection/amplification of one or more enzymes involved in the production of cannabinoids, such as, for example, tetrahydrocannabinolic acid (THCA) synthase and/or cannabidiolic acid (CBDA) synthase.

Abstract: The present invention relates to methods and compositions for identifying, selecting and/or producing a maize plant or plant part having increased fertility. A maize plant or plant part that has been identified, selected and/or produced by any of the methods of the present invention is also provided.

Abstract: Methods and systems are provided for packaging reporter nucleic acid molecules into non-replicative transduction particles for use as reporter molecules. The non-replicative transduction particles can be constructed from viruses and use viral transduction and replication systems. The reporter nucleic acid molecules include a reporter gene, such as a reporter molecule or selectable marker, for detecting target genes or cells. Methods and systems are provided for detection of cells and target nucleic acid molecules using the non-replicative transduction particles as reporter molecules.

Abstract: A structure is heated in a tool. The structure is quenched in the tool such that a shape of the structure is maintained, in which quenching is performed by contacting the structure with a quenching medium.

Abstract: A method for producing a soft magnetic material having both high saturation magnetization and low coercive force, including: preparing an alloy having a composition represented by Compositional Formula 1 or 2 and having an amorphous phase, and heating the alloy at a rate of temperature rise of 10° C./sec or more and holding for 0 to 80 seconds at a temperature equal to or higher than the crystallization starting temperature and lower than the temperature at which Fe—B compounds start to form wherein, Compositional Formula 1 is Fe100-x-yBxMy, M represents at least one element selected from Nb, Mo, Ta, W, Ni, Co and Sn, and x and y are in atomic percent (at %) and satisfy the relational expressions of 10?x?16 and 0?y?8, and Compositional Formula 2 is Fe100-a-b-cBaCubM?c, M? represents at least one element selected from Nb, Mo, Ta, W, Ni and Co, and a, b and c are in atomic percent (at %) and satisfy the relational expressions 10?a?16, 0<b?2 and 0?c?8.

Abstract: A steel strip is produced by melting a steel melt containing (In wt. %): C: 0.1 to <0.3; Mn: 4 to <8; Al: >1 to 2.9; P: <0.05; S: <0.05; N: <0.02; remainder iron including unavoidable steel-associated elements. The steel melt is cast to form a pre-strip or to form a slab and heated to a rolling temperature of 1050 to 1250° C. or in-line rolling out of the casting heat. The pres-strip or slab is hot rolled into a hot strip having a thickness of 12 to 0.8 mm, at a final rolling temperature of 1050 to 800° C. The hot strip is reeled at a temperature of more than 200 to 800° C., pickled, annealed for an annealing time of 1 min to 48 h and at a temperature of 540 to 840° C., and cold rolled at room temperature or elevated temperature in at least one rolling pass.

Abstract: A press hardening steel by a thin slab casting and direct rolling has a tensile strength of 1500 MPa or more. The press hardening steel has a components by weight percent: C: 0.21-0.25%, Si: 0.26-0.30%, Mn: 1.0-1.3%, P?0.01%, S?0.005%, Als: 0.015-0.060%, Cr: 0.25-0.30%, Ti: 0.026-0.030% or Nb: 0.026-0.030% or V: 0.026-0.030%, or a mixture of two or more of the above in any proportion; B: 0.003-0.004%, and N?0.005%. A method for producing the press hardening steel includes following steps: hot metal desulphurization; electric-furnace or converter smelting and refining; continuous casting; descaling, then entering a soaking furnace; heating and soaking; high-pressure water descaling, then entering a rolling mill; hot rolling; cooling; coiling; austenitizing; die deforming and quenching.

Abstract: A press hardening steel by a thin slab and having a tensile strength of 1900 MPa or more includes following components by weight: C: 0.31-0.40%, Si: 0.36-0.44%, Mn: 1.6-2.0%, P?0.006%, S?0.004%, Als: 0.015-0.060%, Cr: 0.36-0.49%, Ti: 0.036-0.045% or Nb: 0.036-0.045% or V: 0.036-0.045% or a mixture of any two or more of the above in any proportion, B: 0.004-0.005%, Mo: 0.26-0.35%, and N?0.005%. A method for producing the press hardening steel includes following steps: molten iron desulphurization; smelting and refining by an electric furnace or converter; continuous casting; descaling treatment before entering a soaking furnace; heating and soaking; high pressure water descaling before entering a rolling mill; hot rolling; cooling; coiling; austenitizing; die deforming and quenching.

Abstract: Provided are a steel sheet having a tensile strength of 950 MPa or more and good toughness and a method for manufacturing the same. The steel sheet has a specific composition and a metallographic structure containing: a ferrite area fraction of 30% or less (including 0%), a tempered martensite area fraction of 70% or more (including 100%), and a retained austenite area fraction of 4.5% or less (including 0%), wherein the average aspect ratio of an iron based carbide, precipitated in tempered martensite grains, having a grain size in the largest 10% is 3.5 or more.

Abstract: Provided are a high-strength steel sheet having high strength of a yield strength of 550 MPa or more and a method for manufacturing the same. The high-strength steel sheet has a specified chemical composition and a microstructure, where observed in a cross section in a thickness direction perpendicular to a rolling direction, including a martensite phase having a volume fraction of 50% to 80%, and a ferrite phase having an average grain diameter of 13 ?m or less, wherein a volume fraction of ferrite grains having an aspect ratio of 2.0 or less with respect to the whole ferrite phase is 70% or more, and wherein an average length in a longitudinal direction (in a width direction of the steel sheet) of the ferrite grains is 20 ?m or less, and a yield strength (YP) of 550 MPa or more.

Abstract: The invention relates to a charging apparatus for the dimensionally stable heat treatment of workpieces (1), in particular those of a disk or ring shape, having a charging rack (10) for horizontally mounting the workpiece (1), wherein at least s three screw-type mandrels or ball joint mandrels (2) are arranged on the charging rack (10), the ball joint mandrels (2) having a base plate (3) with a female thread (7), a screw element (4) with a male thread (7) matched to the female thread (7) and a head that has a spherical segment surface, as well as a pressure piece (5) matched precisely to the spherical segment surface of the head and arranged movably thereon.

Abstract: A method of producing a steel plate member includes: heating a steel plate member to a temperature higher than an austenite transformation finish temperature and subsequently cooling the steel plate member at a cooling rate higher than an upper critical cooling rate; and softening the steel plate member by reheating the steel plate member after the cooling of the steel plate member.

Abstract: Disclosed is a high-strength sheet including: C: 0.15% by mass to 0.35% by mass, total of Si and Al: 0.5% by mass to 3.0% by mass, Mn: 1.0% by mass to 4.0% by mass, P: 0.05% by mass or less, and S: 0.01% by mass or less, with the balance being Fe and inevitable impurities, wherein the steel structure satisfies that: a ferrite fraction is 5% or less, the total fraction of tempered martensite and tempered bainite is 60% or more, the amount of retained austenite is 10% or more, MA has an average size of 1.0 ?m or less, a half-width of the concentration distribution of Mn in the carbon-concentrated region that is equal to the amount of retained austenite is 0.3% by mass or more, and a scattering intensity at the q value of 1 nm?1 in X-ray small angle scattering is 1.0 cm?1 or less.

Abstract: A part obtained by austenitization followed by hot stamping and hardening by holding in the stamping tool a sheet or blank is provided, including, a steel substrate, a precoating having at least one layer of aluminum or aluminum alloy, a microstructure of a steel substrate including martensite and/or bainite, a coating on at least one face of the steel substrate resulting from the interdiffusion between the steel substrate and the pre-coating and an oxide layer overlying the coating, an average content by weight of oxygen between 0 and 0.01 ?m below a surface of the part being less than 25%, and the average percent by weight of oxygen between 0.1 and 0.2 ?m below the surface being less than 10%.

Abstract: A forging method is provided. The forging comprises determining plans of second and third processes for each of a plurality of ingots, categorizing the plurality of ingots into first and second ingot sets, based on the plans of the second and third processes, evaluating the first and second ingot sets using a scoring function, determining an ingot set to be provided to a first heating furnace, based on the evaluating of the first and second ingot sets, and performing a first process, different from the second and third processes, on the ingot set provided to the first heating furnace.

Abstract: Ultrasonic probes containing a plurality of gas delivery channels are disclosed, as well as ultrasonic probes containing recessed areas near the tip of the probe. Ultrasonic devices containing these probes, and methods for molten metal degassing using these ultrasonic devices, also are disclosed.

Abstract: A method of melting an aluminum charge having no more that 4% salt by mass, including during a melting phase, introducing fuel and oxidant via a burner operating at a first firing rate, the fuel and oxidant reacting to form a combustion zone above the aluminum charge, terminating the melting phase and commencing a transition phase when the aluminum charge is nearly completely molten, during the transition phase, reducing the firing rate of the burner to a second firing rate lower than the first firing rate, introducing a non-oxidizing gas at a first velocity to form a non-oxidizing zone between the combustion zone and the aluminum charge, and allowing the aluminum charge to become completely molten, and terminating the transition phase and commencing a tapping phase after the aluminum charge has become completely molten, and during the tapping phase, pouring the molten aluminum charge out of the furnace.

Abstract: A process for the recovery of lithium from minerals rich in lithium and phosphate, the process comprising passing an ore containing one or more minerals rich in lithium and phosphate to an acid leach step thereby producing a pregnant leach solution, subjecting the pregnant leach solution to a series of process steps by which one or more impurity elements are removed, and recovering lithium as a lithium containing salt product, wherein the series of process steps by which one or more impurity elements are removed includes a low pH impurity removal step conducted at an elevated temperature for the precipitation of one or more impurities.

Abstract: Provided is a method for efficiently purifying scandium by separating scandium and impurities from an acidic solution which contains scandium and impurities that include iron. A method for purifying scandium according to the present invention subjects an acidic solution, which contains an element component including at least ion, while containing scandium, to solvent extraction by means of a mixed extractant containing a phosphoric acid-based extractant and a neutral extractant, thereby extracting scandium from the acidic solution. It is preferable that the phosphoric acid-based extractant is contained in the mixed extractant at a mixing molar ratio within the range of from 5% to 50% (inclusive). It is also preferable that the pH of the acidic solution is adjusted to a value within the range of from 0.0 to 2.0 (inclusive) before the solvent extraction.

Abstract: A nickel-free and cobalt-free platinum alloy including, expressed by weight, from 95.0% to 96.0% of Pt, from 0.5% to 4.5% of Ir, from 0.01% to 2% of Au, from 0 to 2% of Ge, and from 0 to 1% of at least one of the alloying elements Ru, Rh, Pd, Sn, Ga, Re, the respective percentages of all of the elements of the alloy adding up to 100%.

Abstract: It is an object of the present invention to provide a copper alloy foil that achieves both electrical conductivity and heat resistance to solve the above-mentioned problem. It is another object of the present invention to provide a copper alloy foil that, when used for a negative electrode current collector of a secondary battery, exhibits improved adhesion to an active material layer. A copper alloy foil contains 0.005 to 0.015 wt % of Sn and has an oxygen content of 0.0020 wt % or less, the balance being Cu and inevitable impurities. A Sn-rich layer is formed on at least one surface layer of the copper alloy foil.

Abstract: An aluminum alloy suitable for casting transmission clutch housings, the aluminum alloy includes about 12 to about 16 weight percent silicon; about 0.0 to about 6.0 weight percent copper; about 0.2 to about 0.5 weight percent magnesium; about 0.1 to about 0.5 weight percent chromium; about 0 to about 1.0 weight percent each of iron, manganese, and zinc; less than 0.1 weight percent each of strontium and phosphorous; and about 0.0 to about 0.25 weight percent other trace elements. Also disclosed is a high pressure die cast article, such as a transmission clutch housing.

Abstract: An Al casting alloy contains the following alloy components: Si: >3.8 to 5.8 wt.-%, Mg: 0.45 to 0.55 wt.-%, Cr: 0.05 to 0.5 wt.-%, Sr: 0.010 to 0.030, Contaminants: <0.1 wt.-%, and is supplemented to 100 wt.-% with Al, in each instance. Preferably, Si is contained at a content of more than 4.0 wt.-%, Mg is contained with a content of at least 0.47 wt.-%, Cr is contained with a content of at least 0.07 wt.-%, and Sr is contained with a content of at least 0.015 wt.-%.

Abstract: An aluminum alloy sheet that exhibits excellent surface quality after anodizing, includes a peritectic element that undergoes a peritectic reaction with at least aluminum, and requires an anodic oxide coating is characterized in that the concentration of the peritectic element in a solid-solution state that is present in the outermost surface area of the aluminum alloy sheet varies in the widthwise direction of the aluminum alloy sheet in the form of a band having a width of 0.05 mm or less, and the difference in the concentration of the peritectic element between adjacent bands is 0.008 mass % or less.

Abstract: This disclosure provides a hot-dip Al—Zn alloy coated steel sheet with excellent corrosion resistance after coating. The presently disclosed hot-dip Al—Zn alloy coated steel sheet has a hot-dip coating layer containing, by mass %, Al: 25% to 80% and Ce: 0.1% to 3%, where the balance is Zn and inevitable impurities.

Abstract: A sintered R-T-B based magnet according to the present disclosure is a sintered R-T-B based magnet containing a main phase crystal grain and a grain boundary phase, the sintered R-T-B based magnet containing: R: not less than 27.5 mass % and not more than 35.0 mass % (R is at least one rare-earth element which always includes Nd and Pr); B: not less than 0.80 mass % and not more than 1.05 mass %; Ga: not less than 0.05 mass % and not more than 1.0 mass %; M: not less than 0 mass % and not more than 2 mass % (where M is at least one of Cu, Al, Nb and Zr); and a balance T (where T is Fe, or Fe and Co) and impurities. A Pr/Nd which is a ratio of a concentration of Pr to a concentration of Nd in a central portion of a main phase crystal grain that is located at a depth of 300 ?m from the magnet surface is lower than 1; and a Pr/Nd which is a ratio of a concentration of Pr to a concentration of Nd in an intergranular grain boundary that is located at a depth of 300 ?m from the magnet surface is higher than 1.

Abstract: The invention is concerned with the fields of cemented carbide materials and ceramic and/or powder-metallurgical process engineering and relates to a sintered cemented carbide granulate such as that which can, for example, be used for the production of wear parts or tools with cemented carbides, and to its use. The object of the present invention is to specify a cemented carbide granulate with which cemented carbide green bodies and cemented carbide sintered bodies that exhibit a high green density and high green strength can be produced, and to specify the use thereof. The object is attained with a sintered cemented carbide granulate which, for the majority of granules, has an inhomogeneous distribution of hard material and metallic binder in the individual granule, wherein the concentration of the metallic binder at the surface of the individual granule is, in total, at least 25% greater than in the interior of the granule.

Abstract: A cast nickel-base superalloy that includes iron added substitutionally for nickel. The cast nickel-base superalloy comprises, in weight percent about 1-6% iron, about 7.5-19.1% cobalt, about 7-22.5% chromium, about 1.2-6.2% aluminum, optionally up to about 5% titanium, optionally up to about 6.5% tantalum, optionally up to about 1% Nb, about 2-6% W, optionally up to about 3% Re, optionally up to about 4% Mo, about 0.05-0.18% C, optionally up to about 0.15% Hf, about 0.004-0.015 B, optionally up to about 0.1% Zr, and the balance Ni and incidental impurities. The superalloy is characterized by a ?? solvus temperature that is within 5% of the ?? solvus temperature of the superalloy that does not include 1-6% Fe and a mole fraction of ?? that is within 15% of the mole fraction of the superalloy that does not include 1-6% Fe.

Abstract: A method for preparing nano-SiO2 reinforced aluminum matrix composites, includes the following: Step-1, powder mixing: mixing aluminum matrix powder with nano-SiO2 powder to obtain raw material powder, wherein the aluminum matrix powder has an average particle size between 30 ?m to 100 ?m, the nano-SiO2 powder has an average particle size between 5 nm to 145 nm, mass percentage of nano-SiO2 in the raw material powder is 0.01% to 5% and the remaining raw material powder is the aluminum matrix powder; Step-2, shaping: press shaping the powder obtained in the Step-1 to obtain base bodies; Step-3, sintering: sintering the base bodies obtained in the Step-2 in an atmosphere of N2 at 550° C. to 660° C., preserving the temperature for a period of 5 min to 60 min, and cooling in a furnace at end of the period under protection of N2 for 0.5 h to 3 h; and Step-4, heat treatment.

Abstract: A casting of a hypereutectic white iron that, in an as-cast form of the casting, has a microstructure that includes a ferrous matrix that contains 12-20 wt. % chromium in solution in the matrix, eutectic chromium carbides dispersed in the matrix, primary chromium carbides dispersed in the matrix, and optionally secondary carbides dispersed in the matrix. The eutectic carbides are 15-25 vol. % of the casting and the primary carbides are 25-35 vol. % of the casting. When present, the secondary carbides are up to 6 vol. % of the casting.

Abstract: A steel material produced via powder metallurgy having a matrix that includes (in wt %) C: 3.9-5.0%, Si: 0.3-2.0%, Mn: 0.3-2.0%, P: 0-<0.035%. S: 0-<0.35%, N: 0-<0.12%. Cr: 11.0-15.0%, Mo: 0.5-2.0%, V: 13.5-16.5%. optionally at lease one element from the group of Nb, Ni, Co, and W, wherein the content of each of Ni, Co, and W is at most 1.0% and the content of Nb is at most 2.0% with the remainder being iron and unavoidable impurities. Hard material panicles may be present in contents of up to 30 wt % in the matrix. A solid semi-finished product is formed by a sintering process or an additive process from a sled alloy powder having this composition that undergoes a heal treatment and is then finished to form component.

Abstract: The invention relates to metal industry and more particularly to alloy for producing steel wheels, as well as to steel wheels made from such alloy for railway transport. The steel comprises the following components, in % wt.: from 0.45% wt. to 0.60% wt. of carbon, from 0.38% wt. to 0.50% wt. of silicon, from 0.80% wt. to 1.00% wt. of manganese, up to 0.15% % wt. of vanadium, from 0.80% wt. to 1.00% wt. of chromium, up to 0.02% wt. of phosphorus, up to 0.015% wt. of sulfur, up to 0.3% wt. of copper, up to 0.25% wt. of nickel, up to 0.04% wt. of aluminum, a balance of iron. The claimed steel alloy for use in producing railway wheels, either by metal working process or by casting methods, has improved mechanical and operational properties.

Abstract: The present invention describes an aluminum rolling method comprising a stage a) of hot rolling at an initial temperature ranging from 450 to 500° C. up to a final temperature ranging from 360 to 400° C., obtaining an aluminum sheet with a thickness between 8 and 12 mm; and a stage b) of cold rolling wherein rolling passes at a temperature ranging from 75 to 120° C. are intercalated with an intermediate sub-stage of heat treatment at a temperature ranging from 340 to 390° C., until obtaining an aluminum sheet with a final thickness between 0.3 and 1 mm. Another object of the invention is the rolled aluminum obtained from the method explained in the present application and its use in manufacturing containers for the cosmetic and perfume industry.