Abstract: A three-compartment water-soluble capsule, each compartment containing a part of a liquid detergent composition, the compartments being arranged side-by-side to provide a central compartment flanked on respective sides by a first and a second side compartment, the capsule being formed from two sheets of water-soluble film, the two sheets of film being sealed together to form a sealing web around each compartment, the sealing web lying in a sealing plane, each of the three compartments extending at a maximum the same distance above and below the sealing plane, wherein the sealing web comprises an annular sealing web defining the periphery of the capsule, and two internal sealing webs which each extend across the capsule, each internal sealing web serving to connect the central compartment with an adjacent side compartment and to separate their contents, wherein each internal sealing web has a continuously curved shape.

Abstract: Photobioreactor devices and units for the production of biomass and remediation of environmental contamination are provided. The bioreactor devices comprise a membrane photobioreactor (PBR), the PBR comprising a liquid medium, at least one photosynthetic microorganism, and at least one outer membrane layer, wherein the membrane layer is comprised of a material that is permeable to gas transfer across the membrane layer; and further comprise a chamber defining a gaseous atmosphere enclosed within, wherein the PBR is located inside the chamber. The devices also comprise a control system which controls the composition of the atmosphere within the chamber. Gas transfer occurs across the membrane layer of the PBR, between the PBR and the atmosphere comprised within the chamber. Systems comprising the devices are provided as well as methods of using the devices for the production of biomass, remediation of wastewater and removal of atmospheric pollutants.

Abstract: Described herein are a system, device, methods and compositions related to generating 3-dimensional cardiac tissues. Also described herein are a system, device, and methods of maturing 3-dimensional cardiac tissues and maintaining their viability in culture.

Abstract: The present invention relates to artificial antigen presenting cell (aAPC) scaffolds to provide cells with specific functional stimulation to obtain phenotypic and functional properties ideal to mediate tumor regression or viral clearance. In particular, the scaffolds of the present invention comprise antigens, such as peptide-MHC (pMHC) class I molecules, and specific combinations of cytokines and co-stimulatory molecules to allow effective expansion and functional stimulation of specific T cells.

Abstract: Disclosed herein are systems and methods that provide for increased carrying capacity of bioreactors using silica polymers. Disclosed is a method that includes supplying nutrients and silica polymers containing microorganisms to a bioreactor to form a first suspension and controlling temperature, pressure, and nutrient conditions in the bioreactor to produce a second suspension with increased carrying capacity as compared to a control bioreactor containing microorganisms without the silica polymers.

Abstract: A perfusion device for a cell culture container, particularly a petri dish, includes an inlet opening for supplying nutrient medium, and an outlet opening for discharging unused nutrient medium. A particularly plate-shaped perfusion region dispenses nutrient medium to cell cultures, and is connected to the inlet opening via a supply line and is connected to the outlet opening via a discharge line. The perfusion region can be inserted into the cell culture container and comprises a geometric structure which connects the supply line and the discharge line in the form of an at least partially, preferably entirely spiral-shaped channel, through which nutrient medium can flow. The geometric structure is spaced apart from the floor of the cell culture container in the inserted state.

Abstract: Provided herein are systems and methods for recycling and supplementing off-gas from a gas fed reaction process. The systems and methods are particularly useful for bioprocesses that convert hydrogen gas into one or more biosynthetic products. By maintaining separate hydrogen and oxygen feed gas streams, and forming a recycle gas that introduces a target component of the supply gas to the bioreactor within a target concentration range, the yields, productivities, and safety profiles of the bioprocess can be enhanced.

Abstract: A fermentation tank includes a tank body, enclosing a cavity therein, having a material inlet and a material outlet; a heat exchange structure, disposed on a wall of the cavity for heat exchanging with the tank body; at least one flow disturbing plate, being an elongated plate, the width direction of the flow disturbing plate being parallel to a radial direction of the tank body, the longitudinal direction of the flow disturbing plate being parallel to a vertical direction, a length of the flow disturbing plate being larger than a width thereof, the flow disturbing plate being arranged in the cavity and connected to the tank body, the flow disturbing plate having a heat exchange channel therein, two ends of the heat exchange channel communicating an exterior respectively so that heat is exchanged between the at least one flow disturbing plate and the cavity.

Abstract: The present invention provides a microorganism inspection system that is capable of instantly inspecting individual containers for microorganism contamination of the containers that are filled with or accommodate medicine and the like. The present invention has a supply means for supplying a predetermined amount of clean air to the inside or the outer surface of a container, a collecting means for collecting the air supplied to the container by the supply means, and a detecting means for detecting microorganisms included in the air collected by the collecting means.

Abstract: This invention concerns an integrated microfluidic system that utilizes microfluidic chip technology to receive a patient sample including cells, expand the cells, reprogram the expanded cells and then store the reprogrammed cells in a microfluidic chip. These microfluidic chips with stored reprogrammed cells may then be used in scenarios of genetic differentiation into specific cell types. Overall this system and workflow is suitable as a hospital based device that will allow the generation of iPSCs from every isolating patient for downstream diagnostic or therapeutic use.

Abstract: The present invention relates generally to microalgae strains that are tolerant to high salinity, the products derived from the high salinity tolerant microalgae strains, and methods of producing high salinity tolerant microalgae strains and their products.

Abstract: A novel method of growing fungi is disclosed which uses an engineered artificial media and produces high density filamentous fungi biomats that can be harvested with a minimum of processing and from which fungal products such as antibiotics, proteins, and lipids can be isolated, the method resulting in lowered fungus cultivation costs for energy usage, oxygenation, water usage and waste stream production.

Abstract: A novel method of growing fungi is disclosed which uses an engineered artificial media and produces high density filamentous fungi biomats that can be harvested with a minimum of processing and from which fungal products such as antibiotics, proteins, and lipids can be isolated, the method resulting in lowered fungus cultivation costs for energy usage, oxygenation, water usage and waste stream production.

Abstract: A method for growing probiotic organisms wherein the growth media includes prebiotics especially selected and prepared to be paired with the probiotic organisms being grown. The prebiotic formula is optimized to grow the desired probiotic organisms, as well as important byproducts of the growth process. Specialized freeze-drying buffers may also be paired with certain probiotic organisms for the freeze-drying process.

Abstract: Disclosed are date palm compositions and methods of use of such compositions. Such compositions may be used as part of a growth medium for culture of microorganisms. In certain embodiments, date palm extracts of the disclosure are used as part a culture medium to grow Lactobacilli.

Abstract: The present disclosure provides methods and reagents for culturing pluripotent stem cells. The method comprises a first step of culturing in a medium comprising inhibitors of GSK-3?, JNK, p38, PKC and Erk1/2, followed by a second step of culturing in a medium comprising inhibitors of GSK-3?, JNK, p38 and PKC, and not comprising an inhibitor of Erk1/2. The medium of the second step may also further comprise dextran sulfate. The pluripotent stem cells produced using the methods and reagents provided may also be differentiated into a cell type of interest. The methods and reagents provided in this disclosure offer robust and scalable technologies for manufacturing the quantities of cells anticipated to be required for widespread patient access.

Abstract: Well-defined, xeno-free culture media which comprise a TGF-beta isoform or the chimera formed between IL6 and the soluble IL6 receptor (IL6RIL6), which are capable of maintaining stem cells, and particularly, human embryonic stem cells, in an undifferentiated state are provided. Also provided are cell cultures comprising the culture media and the stem cells and methods of expanding and deriving embryonic stem cells in such well-defined, xeno-free culture media. In addition, the present invention provides methods of differentiating ESCs or EBs formed therefrom for the generation of lineage specific cells.

Abstract: A method for culturing hepatocytes involves maintenance, proliferation, and passaging of the hepatocytes. The method includes culturing the hepatocytes in a culture medium, which includes a hepatocyte basic medium; CHIR99021, which is a GSK-3 beta inhibitor at a concentration of 0.5-10 ?M, SB 431542 or A83-01, which are RGF beta inhibitors, at a concentration of 0.5-10 ?M, N-acetyl-cysteine at a concentration of 0.25-25 mM; and Oncostatin M at a concentration of 1-100 ng/mL There is no exogenous gene introduced into the hepatocytes, and the genetic background of the hepatocytes is not changed.

Abstract: A method for sorting motile cells includes introducing an initial population of motile cells into an inlet port of a microfluidic channel, the initial population of motile cells having a first average motility; incubating the population of motile cells in the microfluidic channel; and collecting a sorted population of motile cells at an outlet port of the microfluidic channel. The sorted population of motile cells has a second average motility higher than the first average motility.

Abstract: This invention relates to methods for the expansion of T-lymphocytes with a memory-like phenotype in which the intracellular concentration of a memory induction compound, such as 2-hydroxyglutarate (2HG), is increased in order to facilitate the maintenance of a memory-like phenotype. This may be useful for example, in cellular immunotherapy. The memory induction compound may the formula (I) wherein: p is 0 or 1, and when p is 0, Y is —CH2— or —C?, and when p is 1, Y is selected from —CH—, CH2, —NH—, —S, and -0-; —R1 is —H, —(CH2)nCH3, —(CH2)nCH2CO2H, —CH2Ph or —CH2PhOCH2Ph; and when Y is —CH—, CH2, —NH—, —S, or —O—, X is a single bonded group selected from —H, —OH, —NH2, —SH, —(CH2)nCH3 —(CH2)nCH2CO2H, —F, —Cl, —Br, and —I, or a double bonded group selected from ?O and ?S; and when Y is a double bonded —C?, X is —H; and each n is independently 0 to 12.

Abstract: The present disclosure relates to compositions and methods for reducing immune tolerance associated with CAR T cell therapy. Embodiments of the present disclosure include isolated nucleic acid sequence comprising a nucleic acid sequence that encodes modified programmed cell death protein 1 (PD-1) and a nucleic acid sequence that encodes chimeric antigen receptor (CAR).

Abstract: A method of generating an isolated population of non graft versus host disease (GvHD) inducing cells comprising a central memory T-lymphocyte (Tcm) phenotype, the cells being tolerance inducing cells and/or endowed with anti-disease activity, and capable of homing to the lymph nodes following transplantation is disclosed. The method comprising: (a) providing a population of at least 70% memory T cells; (b) contacting the population of memory T cells with an antigen or antigens so as to allow enrichment of antigen reactive cells; and (c) culturing the cells resulting from step (b) in the presence of cytokines so as to allow proliferation of cells comprising the Tcm phenotype. Cells generated by the method, pharmaceutical compositions and methods of treatment are also disclosed.

Abstract: Methods for preparing ex vivo T cell cultures using IL-21 compositions for use in adoptive immunotherapy are described. Addition of IL-21 to cultures of non-terminally differentiated T cells population, either isolated or present in peripheral blood mononuclear cells are exposed to one or more tumor antigens, and in the presence of IL-21 compositions and antigen presenting cells (APCs), the resulting T cell population has an enhanced antigen-specificity, and can be reintroduced into the patient. Methods are also disclosed for identifying tumor antigens by culturing T cell populations exposed to IL-21 compositions and APCs in the presence of tumor material.

Abstract: Compositions comprising synthetic membrane-receiver complexes, methods of generating synthetic membrane-receiver complexes, and methods of treating or preventing diseases, disorders or conditions therewith.

Abstract: Compositions comprising synthetic membrane-receiver complexes, methods of generating synthetic membrane-receiver complexes, and methods of treating or preventing diseases, disorders or conditions therewith.

Abstract: Compositions comprising synthetic membrane-receiver complexes, methods of generating synthetic membrane-receiver complexes, and methods of treating or preventing diseases, disorders or conditions therewith.

Abstract: Provided are methods of isolating and culturing various cells in a living state including peripheral blood mononuclear cells (PBMCs) isolated from peripheral blood, which use a cell membrane permeabilization method. The methods use a streptococcal hemolytic exotoxin, which binds to cholesterol present in a cell membrane so as to make a pore therein, thereby allowing an exogenous protein to be permeated into the cell, and is a technique of isolating and culturing desired cells in a living state by probing a specific intracellular protein and performing flow cytometry (FACS). According to the methods, various intracellular proteins that could not previously be isolated and cultured from a patient's blood as well as various tissues may be targeted, and homogeneous cells may be obtained with high purity and high efficiency.

Abstract: The present invention relates to an in vitro testing platform for the study of metabolic liver disease and related therapeutic strategies, composed of an artificial spheroidal microtissue comprising at least hepatocytes and hepatic stellate cells, and at least one type of hepatic inflammatory cells and further medium and reagents capable to establish and simulate different stages of metabolic liver disease and their progression and testing preventive and therapeutic strategies by pharmacological and/or dietary interventions.

Abstract: The present invention provides methods of differentiating pericytes from pluripotent stem cells comprising culturing steps in chemically defined culture medium. A population of exogenously derived pericytes from PSCs are also contemplated. Further uses of the exogenously cultured pericytes for an in vitro disease model or in vitro angiogenesis assay are contemplated, including an in vitro 3D model of vasculature.

Abstract: Methods are disclosed for reprogramming a somatic cell, including an adherent cell and a cell in suspension, into an induced pluripotent stem comprising expressing exogenous Sox-2, exogenous Klf-4, exogenous Oct3/4 from DNA that has not integrated into the genome of the somatic cell, suppressing p53 activity within the somatic cell, and exposing the somatic cell to reprogramming-assistance factors comprising an exogenous Alk-5 inhibitor, an exogenous histone deacetylase inhibitor, and an exogenous activator of glycolysis. Compositions and kits for use in such methods are also disclosed as are cells made by such a method.

Abstract: An object of the present invention is to provide a method for selecting a cell strain in which reduction of a recombinant protein is suppressed. The present invention relates to a method for selecting a cell, comprising a first step: measuring the expression level of a gene in a cell, the gene being at least one gene selected from genes comprising any one of the base sequences represented by SEQ ID NOS: 1 to 16 or orthologous genes thereof; and a second step: comparing the expression level of the gene measured in the first step with a control value of the expression level of the gene in a control cell, and evaluating the expression level capable of suppressing reduction of a recombinant protein based on a difference therebetween.

Abstract: Genetically modified proteins with uricolytic activity are described. Proteins comprising truncated urate oxidases and methods for producing them, including PEGylated proteins comprising truncated urate oxidase are described.

Abstract: DGA1 catalyzes the final enzymatic step for converting acyl-CoA and 1,2-diacylglycerol to triacylglycerols (TAG) and CoA in yeast. Disclosed are methods for expression in an oleaginous yeast host of polynucleotide sequences encoding DGA1 from Rhodosporidium toruloides, Lipomyces starkeyi, Aurantiochytrium limacinum, Aspergillus terreus, or Claviceps purpurea. Also described herein are engineered recombinant host cells of Yarrowia lipolytica comprising heterologous DGA1 polynucleotides encoding DGA1 proteins, or functionally active portions thereof, having the capability of producing increased lipid production and possessing the characteristic of enhanced glucose consumption efficiency.

Abstract: Methods and compositions for producing fatty acid derivatives, for example, fatty esters, and commercial fuel compositions comprising fatty acid derivatives are described.

Abstract: The present invention relates to new Transcription Activator-Like Effector proteins and more particularly new Transcription Activator-Like Effector Nucleases (TALENs) that can efficiently target and process nucleic acids. The present invention also concerns methods to use these new Transcription Activator-Like Effector proteins. The present invention also relates to vectors, compositions and kits in which Transcription Activator-Like Effector proteins of the present invention are used.

Abstract: This disclosure provides methods and compositions for genetically manipulating genes and cells.

Abstract: Paired CRISPR nickase ribonucleoproteins engineered to target immune-related genomic loci and methods of using said ribonucleoproteins to modify the immune-related genomic loci.

Abstract: The present invention relates to GH61 polypeptide variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.

Abstract: In certain embodiments, the present disclosure provides compositions and methods for treating Lafora Disease.

Abstract: The present invention relates to alpha-amylase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.

Abstract: The present invention relates to isolated polypeptides having cellulolytic enhancing activity, catalytic domains, cellulose binding domains and polynucleotides encoding the polypeptides, catalytic domains or cellulose binding domains. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains or cellulose binding domains.

Abstract: The present invention relates to polypeptides having xanthan degrading activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

Abstract: The present invention relates to mixtures comprising a polypeptide or a plurality of polypeptides having biomass-degrading activity that is solubilized from an inclusion body, and retaining biomass-degrading activity, and methods for producing and using the same. The invention described herein provides methods for increasing the yield of recombinant protein with biomass-degrading activity that can be isolated from host cells.

Abstract: Polypeptides, viruses, methods and compositions provided herein are useful for the selective elimination of senescent cells. Method aspects include methods for inducing apoptosis in a senescent cell comprising administering to the cell a polynucleotide, virus, host cell, or pharmaceutical composition described herein. Other methods include expressing a pro-apoptotic gene in a senescent cell comprising administering to the cell the polynucleotide, virus, or pharmaceutical composition as described herein.

Abstract: Composition comprising purified recombinant selenoproteins, such an antibodies and enzymes, are provided. Method of producing such recombinant polypeptides and bacterial strains for the same are likewise provided.

Abstract: The present invention relates to novel nucleic acid sequences encoding bacterial xylose isomerases that upon transformation of a eukaryotic microbial host cell, such as yeast, to confer to the host cell the ability of isomerising xylose to xylulose. The nucleic acid sequences encode xylose isomerases that originate from bacteria such as Eubacterium sp., Clostridium cellulosi and others. The invention further relates to fermentation processes wherein the transformed host cells ferment a xylose-containing medium to produce ethanol or other fermentation products.

Abstract: Provided herein are methods of capturing a nucleic acid comprising a target oligonucleotide sequence from a library of nucleic acid that include: contacting a library of nucleic acids comprising a nucleic acid comprising a target oligonucleotide sequence with a probe comprising a sequence that is complementary to the target oligonucleotide sequence, wherein the contacting is performed in a tetramethylammonium chloride (TMAC)-based buffer at a temperature of about 60° C. to about 70° C., and the contacting results in the hybridization of the target oligonucleotide sequence to the sequence that is complementary to the target oligonucleotide sequence, to thereby generate a hybridization product; and isolating the hybridization product from nucleic acids in the library that do not comprise the target oligonucleotide sequence. Also provided are compositions useful for performing these methods.

Abstract: Provided is a method for depleting host nucleic acid in a biological sample, said sample having been previously obtained from an animal host, said method comprising the steps of (a) adding a cytolysin, or an active variant thereof, to said sample; and (b) carrying-out a process to physically deplete nucleic acid released from host cells within said sample or otherwise render such nucleic acid unidentifiable.

Abstract: A system for extracting analytes from a biological sample, which includes: an electronic pipette having pipette cones with a tip; a well support; a pipette holder including: a base which can removably house each well support; a pipette support into which the pipette is inserted, and which can move relative to the base between a first position in which the tips of the cones are inserted in a well of the support and at least one second position in which the tips are outside the wells; a housing facing the pipette cones above their tip when the pipette support is in the first position, and facing the tips of the pipette cones when the pipette support is in the second position; and a magnetized part removably inserted in the housing.

Abstract: The present disclosure is directed to systems, devices and methods for nucleic acid or protein purification and imaging. A system is provided including a cartridge comprising a sample input area configured to hold a sample, comprising a plurality of hybridized complexes comprising a plurality of target molecules each hybridized with probes and a plurality of non-hybridized probes. The cartridge may also include a first binding chamber configured with first magnetic beads to receive and bind the sample, a first elution channel configured to receive the first magnetic beads and elute the sample from the first magnetic beads, a second binding chamber configured with second magnetic beads to receive and bind the sample, a second elution channel configured to receive the second magnetic beads and elute the sample from the second magnetic beads, and a binding area configured to receive the eluted sample and hold molecules for imaging.