Patents Issued in January 9, 2020
  • Publication number: 20200010787
    Abstract: An apparatus for transferring bio-ink onto a target having a slide defining a receiving area of a film of fluid containing inhomogeneities, a laser source associated with controlled diversion means and an optical block for focusing in a plane of the fluid film in order to apply a local pulse, wherein the apparatus also comprises imaging means and means for analyzing images in order to recognize the geometric positions of the inhomogeneities in the film, and an observable feature of each of the inhomogeneities (size, shape factor, type of particles, age of the particle, density, type of biomaterial, molecule, etc.) recognized by the appropriate analysis, means. The apparatus further comprises selection means for selecting at least one of the inhomogeneous areas, and means for controlling the diversion in order to direct the laser beam toward the position of the inhomogeneous area and trigger the firing of the laser.
    Type: Application
    Filed: March 8, 2018
    Publication date: January 9, 2020
    Applicants: Poietis, Poietis
    Inventors: Bertrand Viellerobe, Romain Vaucelle, Fabien Guillemot
  • Publication number: 20200010788
    Abstract: A method, apparatus, and system are provided for the printing and maturation of living tissue in an Earth-referenced reduced gravity environment such as that found on a spacecraft or on other celestial bodies. The printing may be three-dimensional structures. The printed structures may be manufactured from low viscosity biomaterials.
    Type: Application
    Filed: July 19, 2019
    Publication date: January 9, 2020
    Inventors: John C. Vellinger, Eugene Boland, Michael A. Kurk, Krystal Milliner, Nester Samuel Logan, Carlos Chang
  • Publication number: 20200010789
    Abstract: Systems and methods for the efficient agitation of tissue samples. A device may include a plurality of chambers that each receives samples therein. The plurality of chambers may be uniformly spaced with respect to a least one dimension, to form a one dimensional or two dimensional array. Each of the chambers may include an opening and an agitator device in fluid contact with the sample disposed within the chamber. The agitator devices may include a micromotor which provides rotational motion to a shaft and an impeller fixed to the shaft such that the impeller and the shaft rotate together upon provision of the rotational motion by the micromotor. The system may include an electrical energy source electrically coupled to the plurality of micromotors to rotate the impellers sufficient to agitate the sample as required for a particular activity (e.g., homogenization, lysis).
    Type: Application
    Filed: February 1, 2018
    Publication date: January 9, 2020
    Inventors: Robert W. Doebler, Mark Brown, Tanya Ferguson, Bruce Irvine, James D. Sterling
  • Publication number: 20200010790
    Abstract: The present invention provides for a method and transport system for conditioning micro algae grown in a commercial farm and transporting to a final destination while maintaining conditions that allow the microalgae to remain alive and healthy during transit.
    Type: Application
    Filed: July 3, 2019
    Publication date: January 9, 2020
    Inventor: MARTIN L. JOHNSON
  • Publication number: 20200010791
    Abstract: In one aspect, a method of cell processing is disclosed, which includes disposing a plurality of cells on a substrate across which a plurality of projections are distributed and an electrically conductive layer at least partially coating said projections, exposing the cells to a cargo to be internalized by the cells, irradiating the substrate surface (and in particular the projections) with continuous wave or pulsed laser radiation. For example, one or more laser pulses having a pulse width in a range of about 1 ns to about 1000 ns can be applied so as to facilitate uptake of the cargo by at least a portion of the cells (e.g., the cells positioned in the vicinity of the projections (e.g., within hundreds of nanometer (such as less than 100 nm) of the projections)). In some embodiments, the laser pulses have a pulse width in a range of about 10 ns to about 500 ns, e.g., in a range of about 5 ns to about 50 ns.
    Type: Application
    Filed: January 17, 2019
    Publication date: January 9, 2020
    Inventors: Eric Mazur, Nabiha Saklayen, Marinna Madrid, Marinus Huber, Valeria Nuzzo
  • Publication number: 20200010792
    Abstract: A storage assembly for storing a plurality of specimens includes a frame, a plurality of modular storage units for perfusion and/or incubation of one or more specimens removably coupled to the frame, a sample transfer apparatus configured to retrieve a specimen holder from a chosen modular storage unit of the plurality of modular storage units, and a control unit communicatively coupled to the sample transfer apparatus. The control unit is configured to cause the sample transfer apparatus to retrieve a specimen from a modular storage unit of the plurality of modular storage units and deliver the specimen to a delivery position.
    Type: Application
    Filed: July 3, 2019
    Publication date: January 9, 2020
    Applicant: Advanced Solutions Life Sciences, LLC
    Inventors: Michael W. Golway, Scott Douglas Cambron
  • Publication number: 20200010793
    Abstract: The present invention relates to a method of preparing a strain of sugar fermenting Saccharomyces cerevisiae with capability to ferment xylose, wherein said method comprises different procedural steps. The method comprises mating a first sporulated Saccharomyces cerevisiae strain with a second Saccharomyces cerevisiae haploid strain. Thereafter, screening for mated cells is performed, growing such mated cells, and verifying that mated cells exhibit basic morphology by microscopic inspection. Thereafter, creation of a mixture of the mated cells is performed, subjecting the mixture to continuous chemostat lignocellulose cultivation and obtaining the sugar fermenting Saccharomyces cerevisiae cells with capability to ferment xylose is performed. The invention also comprises strains obtained by said method.
    Type: Application
    Filed: July 3, 2019
    Publication date: January 9, 2020
    Inventor: Nicklas Bonander
  • Publication number: 20200010794
    Abstract: The invention relates to methods for inducing sporulation in Bacillus coagulans strains wherein excessive sporulation is induced by presence of certain nutrients and minerals up to a level of 109 spores/ml. The spores are tadpole-like endospores and the method discloses specific parameters of growth medium and incubation conditions to be used to induce sporulation in Bacillus coagulans strains.
    Type: Application
    Filed: March 8, 2018
    Publication date: January 9, 2020
    Inventor: Vinay RALE
  • Publication number: 20200010795
    Abstract: Provided herein are engineered cells, comprising: a chemical or biological moiety covalently bound to a cell surface glycan, wherein the chemical or biological moiety is selected from the group consisting of small molecule, polynucleotide, polypeptide, and antibody. Also provided are compositions comprising these engineered cells and methods of making and using the same.
    Type: Application
    Filed: February 1, 2018
    Publication date: January 9, 2020
    Inventors: Peng Wu, Jie Li, Yiran Zhou, Mingkuan Chen
  • Publication number: 20200010796
    Abstract: The present invention relates to a culture medium, preferably a cell culture medium, comprising at least one dipeptide, the dipeptide comprising asparagine (Asn) and glutamine (Gln). The invention further relates to the use of a culture medium of the invention for culturing cells, preferably plant cells, animal cells or mammalian cells. Another aspect of the invention relates to a method of manufacturing a cell culture product comprising the steps of (i) providing a cell capable of producing said cell culture product; (ii) contacting said cell with a culture medium according to the invention; and (iii) obtaining said cell culture product from said culture medium or from said cell.
    Type: Application
    Filed: March 2, 2018
    Publication date: January 9, 2020
    Applicant: Evonik Degussa GmbH
    Inventors: Friedhelm MERZ, Andreas KARAU, Thomas HERMANN, Guenter KNAUP
  • Publication number: 20200010797
    Abstract: The invention provides methods for producing arrays of organoids, the arrays thereof and uses of such arrays.
    Type: Application
    Filed: September 15, 2017
    Publication date: January 9, 2020
    Inventors: Sylke HOEHNEL, Nathalie BRANDENBERG, Matthias LUTOLF
  • Publication number: 20200010798
    Abstract: Microgel particles for use in a three-dimensional cell growth medium are described. The microgel particles may be swellable and may have properties conducive to improved function and health of cells distributed within the three-dimensional cell growth medium. Related compositions, systems, and methods are also described. Also provided is a plurality of microgel particles and a liquid cell culture medium, wherein the microgel particles are swelled with the liquid cell culture medium to form a granular gel. Also provided is a method of preparing a three-dimensional cell growth medium is disclosed. The method may comprise: mixing a plurality of microgel particles, such as those described above, in a liquid cell culture medium. Also provided is a method of placing cells in a three-dimensional cell growth medium is disclosed. Also provided is a method of synthesizing a protein is disclosed.
    Type: Application
    Filed: March 9, 2018
    Publication date: January 9, 2020
    Inventors: Wallace Gregory Sawyer, Sangwoo Park, Christopher S. O'Bryan, Christopher P. Kabb, Brent S. Sumerlin, Thomas Ettor Angelini
  • Publication number: 20200010799
    Abstract: Photoreceptor scaffolds that can be used for transplantation of organized photoreceptor tissue, with or without retinal pigment epithelial cells, which may improve grafted cell survival, integration, and functional visual rescue are disclosed herein. The scaffolds include a cell support layer having at least one cube-shaped reservoir fluidly connected to a plurality of through-holes and at least one cell in the at least one cube-shaped reservoir.
    Type: Application
    Filed: July 9, 2019
    Publication date: January 9, 2020
    Inventors: Zhenqiang Ma, Juhwan Lee, Yei Hwan Jung, Michael Phillips, David Gamm, Shaoqin Gong, Inkyu Lee
  • Publication number: 20200010800
    Abstract: The present invention relates to a method for controlling differentiation of pluripotent stem cells, which method comprises selecting a laminin or a fragment thereof based on binding affinity for the pluripotent stem cells and inducing differentiation of the pluripotent stem cells in the presence of the laminin or a fragment thereof. Here, the binding affinity for cells can be assessed by time-course observation of the survival rate and motility of the cells. According to the present invention, a cell population containing any desired proportion of differentiated cells can be produced from pluripotent stem cells in a simple manner. The cell population obtained by this production method is very useful for cell therapy-based treatment strategies for diseases.
    Type: Application
    Filed: January 31, 2018
    Publication date: January 9, 2020
    Inventors: Kohji Nishida, Kiyotoshi Sekiguchi, Ryuhei Hayashi, Shun Shibata
  • Publication number: 20200010801
    Abstract: The present invention provides a production method of a retinal pigment epithelial cell containing the following steps: (1) a first step for culturing a pluripotent stem cell in a medium containing at least one kind selected from the group consisting of an FGF receptor inhibitor and an MEK inhibitor for a period of not more than 30 days, and (2) a second step for culturing the cell obtained in the first step in a medium containing at least one kind selected from the group consisting of a Rho signal transduction pathway inhibitor and an apoptosis inhibitor to form a retinal pigment epithelial cell. The method can produce retinal pigment epithelial cells from pluripotent stem cells more efficiently and conveniently.
    Type: Application
    Filed: March 8, 2018
    Publication date: January 9, 2020
    Applicants: SUMITOMO DAINIPPON PHARMA CO., LTD., HEALIOS K.K.
    Inventor: Takao KURODA
  • Publication number: 20200010802
    Abstract: A method for preparing a supplement from mesenchymal cell cultures of Wharton's jelly including factors to favor the culture growth of cells from cutaneous system, in vitro, and to methods for producing epidermal, dermal, or cutaneous equivalents, and which may also be used as supplement for the proliferation and activation of autologous fibroblasts for subsequent intradcrmal use.
    Type: Application
    Filed: September 25, 2017
    Publication date: January 9, 2020
    Inventors: Andrés Eliú CASTELL RODRÍGUEZ, Miguel Ángel HERRERA ENRÍQUEZ, Gabriela PINÓN ZÁRATE, Katia JARQUÍN YÁNEZ, Casandra CHAIRES ROSAS, Rosa María ARELLANO OLIVARES
  • Publication number: 20200010803
    Abstract: Disclosed herein are immunostimulatory cells recombinantly engineered for adoptive cellular therapy. Additionally provided are pharmaceutical compositions comprising such immunostimulatory cells and methods of using such immunostimulatory cells to treat cancer or pathogen infections in a subject in need thereof.
    Type: Application
    Filed: March 7, 2018
    Publication date: January 9, 2020
    Applicant: Memorial Sloan Kettering Cancer Center
    Inventor: Prasad S. Adusumilli
  • Publication number: 20200010804
    Abstract: Provided is a T cell receptor containing, as ? chain complementarity determining regions, respective amino acid sequences of CDR1 to CDR3 respectively shown in SEQ ID NOs: 1 to 3, or respective amino acid sequences of CDR1 to CDR3 respectively shown in SEQ ID NOs: 4 to 6, as ? chain complementarity determining regions, respective amino acid sequences of CDR1 to CDR3 respectively shown in SEQ ID NOs: 7 to 9, or respective amino acid sequences of CDR1 to CDR3 respectively shown in SEQ ID NOs: 10 to 12, and capable of binding to a peptide having the amino acid sequence shown in SEQ ID NO: 27 or a complex of the peptide and HLA-A24.
    Type: Application
    Filed: February 5, 2018
    Publication date: January 9, 2020
    Inventors: Tetsuya Nakatsura, Toshiaki Yoshikawa, Yasushi Uemura, Kyoko Fukuda, Shin Kaneko, Atsutaka Minagawa, Yoshiaki Kassai, Atsushi Matsuda
  • Publication number: 20200010805
    Abstract: Disclosed are a medium composition for culturing mesenchymal stem cells for the treatment of cancer which can inhibit proliferation of cancer cells, while maintaining differentiation capability and activity thereof, and a method for producing mesenchymal stem cells for the treatment of cancer using the composition. More particularly, disclosed are a medium composition containing vitamin C and aspirin for producing mesenchymal stem cells having improved inhibitory activity against proliferation of cancer cells, and a method for producing mesenchymal stem cells for the treatment of cancer using the composition.
    Type: Application
    Filed: July 28, 2017
    Publication date: January 9, 2020
    Inventor: Jeong Chan RA
  • Publication number: 20200010806
    Abstract: Provided are methods and compositions for co-culturing hepatocytes in the presence of growth-arrested stromal cells, resulting in maintenance of hepatocyte function and phenotype for an extended period of time in culture comprising at least 30 days.
    Type: Application
    Filed: January 24, 2018
    Publication date: January 9, 2020
    Applicant: PhoenixSongs Biologicals, Inc.
    Inventors: Marsha Lynn Roach, Richard Harold Malavarca, Shiloh Jesse Barfield
  • Publication number: 20200010807
    Abstract: Provided are: a method for producing biliary epithelial progenitor cells, said method comprising a step for culturing hepatoblasts in a medium containing TGF? and EGF; and a method for constructing a three-dimensional duct-like structure of biliary epithelial progenitor cells, said method comprising a step for culturing hepatoblasts or biliary epithelial progenitor cells in a medium containing HGF, EGF, a Notch inhibitor and a GSK3 inhibitor in the presence of a three-dimensional scaffold material.
    Type: Application
    Filed: November 21, 2017
    Publication date: January 9, 2020
    Inventors: Kenji OSAFUNE, Satoshi MATSUI
  • Publication number: 20200010808
    Abstract: Disclosed is a new process for the production of recombinant proteins, by transient transfection of suspension-grown human embryonic kidney cells (293 cell line and its genetic variants) with an expression vector, using polyethylenimine (PEI) as a transfection reagent. In a preferred embodiment, the process uses 293E cells expressing the Epstein-Barr virus (EBV) EBNA 1 protein, in combination with an oriP-based episomal expression vector having an improved cytomegalovirus expression cassette comprising the CMV5 promoter. The process combines in a single step the cell growth, transfection and protein expression, is carried out without changing the culture medium, and allows to achieve high expression levels in a short period of time. The process may be carried out in a serum-free, low-protein culture medium, is easily scalable, compatible with continuous production processes, and fully adapted to high-throughput production of milligram quantities of recombinant proteins.
    Type: Application
    Filed: August 27, 2019
    Publication date: January 9, 2020
    Applicant: NATIONAL RESEARCH COUNCIL OF CANADA
    Inventors: Yves Durocher, Amine Kamen, Sylvie Perret, Phuong Pham
  • Publication number: 20200010809
    Abstract: Methods for mimicking a tumor microenvironment in vitro are provided. The methods comprise indirectly applying a shear stress upon at least one tumor cell type plated on a surface within a cell culture container. Methods for mimicking tumor metastasis and methods for testing drugs or compounds in such systems are also provided.
    Type: Application
    Filed: February 22, 2019
    Publication date: January 9, 2020
    Inventors: Brian R. Wamhoff, Brett R. Blackman, Robert A. Figler, Daniel G. Gioeli, Michael B. Simmers
  • Publication number: 20200010810
    Abstract: Compositions and methods are provided for making and using a cell having expressed on its outer plasma membrane surface an Anchor designed to form a first complex with a selected Linker. The Linker is designed to form a second complex with in a target protein that is not recognized by the Anchor. These cells can be primary cells or immortalized cells, they function as fusion partners to create hybridoma cells. The cells can be designed to bind a secreted targeted protein, e.g., a secreted antibody, to the cell surface via an immune complex formed by the Anchor-Linker and permit the identification of the existence, antigen specificity, and antigen binding affinity, titer, amount, or biological activity of the target protein. In certain embodiments, the Linker is of a species heterologous to the species of the Anchor; and the target protein is of a species heterologous to the Linker and to the Anchor.
    Type: Application
    Filed: March 23, 2018
    Publication date: January 9, 2020
    Inventors: Scott K. Dessain, Rama Devudu Puligedda
  • Publication number: 20200010811
    Abstract: The invention concerns a modified enveloped virus wherein said virus has at least one anti-tumor, tumor-specific peptide non-genetically attached to or inserted in/through the viral envelope; a pharmaceutical composition comprising same; and a method of treating cancer using same.
    Type: Application
    Filed: September 6, 2017
    Publication date: January 9, 2020
    Applicant: VALO THERAPEUTICS OY
    Inventors: Vincenzo Cerullo, Cristian Capasso, Erkko Ylosmaki
  • Publication number: 20200010812
    Abstract: An alcohol dehydrogenase of sequence ID numbers 2, 3 or 4 containing metal ions and a quinone cofactor, or in addition, a functional variant exhibiting a sequence identity of at least 80%, preferably at least 86%, especially preferred at least 89% and at least one redox cofactor for the transformation of at least one trichothecene exhibiting a hydroxyl group on the C-3 atom, as well as a method for the enzymatic transformation of trichothecenes and a trichothecene-transforming additive.
    Type: Application
    Filed: September 20, 2019
    Publication date: January 9, 2020
    Applicant: Erber Aktiengesellschaft
    Inventors: Eva-Maria BINDER, Barbara Weber, Claudia Bernard
  • Publication number: 20200010813
    Abstract: The disclosure relates to acyl-ACP reductase (AAR) enzyme variants that result in improved fatty aldehyde and fatty alcohol production when expressed in recombinant host cells. The disclosure further relates to methods of making and using such AAR variants for the production of fatty alcohol compositions having particular characteristics.
    Type: Application
    Filed: February 11, 2019
    Publication date: January 9, 2020
    Inventors: Mathew Rude, Na TRINH, Andreas Schirmer, Jacob Gano
  • Publication number: 20200010814
    Abstract: An enzyme protein that allows production of an optically active ?-hydroxyamino acid, specifically a monooxygenase having two kinds of hetero (different) subunits. Also, systems and methods using the monooxygenases.
    Type: Application
    Filed: September 26, 2019
    Publication date: January 9, 2020
    Applicants: KANEKA CORPORATION, Kyoto University
    Inventors: Masutoshi NOJIRI, Yoshihiko YASOHARA, Makoto HIBI, Jun OGAWA
  • Publication number: 20200010815
    Abstract: The present invention provides engineered pantothenate kinase (PanK) enzymes, polypeptides having PanK activity, and polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. Methods for producing PanK enzymes are also provided. The present invention further provides compositions comprising the PanK enzymes and methods of using the engineered PanK enzymes. The present invention finds particular use in the production of pharmaceutical compounds.
    Type: Application
    Filed: July 2, 2019
    Publication date: January 9, 2020
    Inventors: Margie Tabuga Borra-Garske, Oscar Alvizo, Lillian Jasmine Miller, Jesse B. Slaton, Aksiniya Lyubenova Petkova, Nandhitha Subramanian, Joshua Kolev, Anna Fryszkowska, Agustina Rodriguez-Granillo, Grant Murphy
  • Publication number: 20200010816
    Abstract: The present disclosure relates to the seminal discovery of a generation and use of genetically engineered Vibrio sp. Provided is the use of the genetically engineered bacteria for the construction, maintenance, manipulation, and/or propagation of DNA constructs; protein expression; protein secretion; vectors and other metabolic tools; metabolic engineering; expression of cellular extracts for cell-free biology; shuttle vectors; cloning vectors; and for synthetic biology applications. The disclosure also relates to the use of the replication machinery of Vibrio sp. as a cloning or expression vector for replication of recombinant DNA constructs. The disclosure also relates to methods of use of the above.
    Type: Application
    Filed: July 29, 2019
    Publication date: January 9, 2020
    Inventors: Matthew T Weinstock, Christopher M. Wilson, Eric D. Hesek
  • Publication number: 20200010817
    Abstract: The invention relates to the field of genetic engineering tools, methods and techniques for gene or genome editing. Specifically, the invention concerns isolated polypeptides having nuclease activity, host cells and expression vectors comprising nucleic acids encoding said polypeptides as well as methods of cleaving and editing target nucleic acids in a sequence-specific manner. The polypeptides, nucleic acids, expression vectors, host cells and methods of the present invention have application in many fields of biotechnology, including, for example, synthetic biology and gene therapy.
    Type: Application
    Filed: April 30, 2019
    Publication date: January 9, 2020
    Applicant: Wageningen Universiteit
    Inventor: John VAN DER OOST
  • Publication number: 20200010818
    Abstract: Engineered nucleases are promising tools for genome manipulation and determining off-target cleavage sites of these enzymes is of great interest. This disclosure provides in vitro selection methods that interrogate 1011 DNA sequences for their ability to be cleaved by active nucleases, e.g., ZFNs and TALENs. The method revealed hundreds of thousands of DNA sequences that can be cleaved in vitro by two ZFNs, CCR5-224 and VF2468, which target the endogenous human CCR5 and VEGF-A genes, respectively. Analysis of the identified sites in cultured human cells revealed CCR5-224-induced mutagenesis at nine off-target loci. This disclosure provides an energy compensation model of ZFN specificity in which excess binding energy contributes to off-target ZFN cleavage. It was also observed that TALENs can achieve cleavage specificity similar to or higher than that observed in ZFNs.
    Type: Application
    Filed: June 14, 2019
    Publication date: January 9, 2020
    Applicant: President and Fellows of Harvard College
    Inventors: David R. Liu, John Paul Guilinger, Vikram Pattanayak
  • Publication number: 20200010819
    Abstract: The present invention relates to a novel cellulase, the use of the novel cellulase in various applications such as detergent compositions and a composition comprising the novel cellulase.
    Type: Application
    Filed: March 22, 2018
    Publication date: January 9, 2020
    Inventors: Claudia JAKOB, Timothy O'CONNELL, Helge JOCHENS, Frank WALLRAPP, Michael HOESL, Andreas KOHL, Thomas EISELE, Jonathan BEST, Andrew Thomas COOK, Panagiotis KOTSAKIS, Dietmar Andreas LANG, Neil James PARRY, Ilaria SAMBI
  • Publication number: 20200010820
    Abstract: The present invention relates to novel human coagulation Factor VIIa variants having coagulant activity as well as polynucleotide constructs encoding such variants, vectors and host cells comprising and expressing the polynucleotide, pharmaceutical compositions, uses and methods of treatment.
    Type: Application
    Filed: September 20, 2019
    Publication date: January 9, 2020
    Inventors: Henrik Oestergaard, Ole Hvilsted Olsen, Katrine Skaarup Larsen, Henning Stennicke
  • Publication number: 20200010821
    Abstract: Cell-targeted serine protease constructs are provided. Such constructs can be used in methods for targeted cell killing such as for treatment cell of proliferative diseases (e.g., cancer). In some aspects, recombinant serine proteases, such as Granzyme B polypeptides, are provided that exhibit improved stability and cell toxicity. Methods and compositions for treating lapatinib or trastuzumab-resistant cancers are also provided.
    Type: Application
    Filed: September 19, 2019
    Publication date: January 9, 2020
    Applicant: Research Development Foundation
    Inventors: Michael G. ROSENBLUM, Khalid Amanali MOHAMEDALI, Lawrence H. CHEUNG
  • Publication number: 20200010822
    Abstract: The invention is directed to a santalene synthase, to a nucleic acid encoding said santalene synthase, to an expression vector comprising said nucleic acid, to a host cell comprising said expression vector, to a method of preparing santalene, to a method of preparing santalol and to a method of preparing a santalene synthase. The invention is further directed to an antibody specific for the santalane synthase.
    Type: Application
    Filed: March 2, 2018
    Publication date: January 9, 2020
    Inventors: Martinus Julius BEEKWILDER, Adèle Margaretha Maria Liduina VAN HOUWELINGEN, Hendrik Jan BOSCH, Georg Friedrich LENTZEN, Elena MELILLO, Hendrik Wouter WISSELINK
  • Publication number: 20200010823
    Abstract: The present invention provides engineered phosphopentomutase (PPM) enzymes, polypeptides having PPM activity, and polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. Methods for producing PPM enzymes are also provided. The present invention further provides compositions comprising the PPM enzymes and methods of using the engineered PPM enzymes. The present invention finds particular use in the production of pharmaceutical compounds.
    Type: Application
    Filed: July 2, 2019
    Publication date: January 9, 2020
    Inventors: Scott J. Novick, Xiang Yi, Nikki Dellas, Oscar Alvizo, Jovana Nazor, Da Duan, Vesna Mitchell, Jonathan Vroom, Santhosh Sivaramakrishnan, Nandhitha Subramanian, Jeffrey C. Moore, Mark Huffman, Agustina Rodriguez-Granillo, Deeptak Verma, Grant Murphy, Nicholas M. Marshall, Jay Russell, Keith A. Canada
  • Publication number: 20200010824
    Abstract: Disclosed herein are methods of producing hexoses from saccharides by enzymatic processes. The methods utilize fructose 6-phosphate and at least one enzymatic step to convert it to a hexose.
    Type: Application
    Filed: August 16, 2019
    Publication date: January 9, 2020
    Applicant: BONUMOSE LLC
    Inventors: Daniel Joseph WICHELECKI, Edwin O. ROGERS
  • Publication number: 20200010825
    Abstract: The invention provides methods for the synthesis of oligosaccharides comprising an aminooxy group. The invention further provides oligosaccharides comprising an aminooxy group, methods for coupling oligosaccharides comprising an aminooxy group to glycoproteins, and oligosaccharide-protein conjugates. Also provided are methods of treating a lysosomal storage disorder in a mammal by administration of an oligosaccharide-protein conjugate.
    Type: Application
    Filed: February 6, 2019
    Publication date: January 9, 2020
    Inventors: Yunxiang ZHU, Seng H. CHENG, Canwen JIANG, Luis Z. AVILA
  • Publication number: 20200010826
    Abstract: Embodiments disclosed herein relate to methods for purifying, activating, and expanding T cells, and subsets thereof.
    Type: Application
    Filed: July 26, 2017
    Publication date: January 9, 2020
    Applicant: Biomagnetic Solutions LLC
    Inventors: Paul A. LIBERTI, Dustin W. RITTER, Todor R. KHRISTOV
  • Publication number: 20200010827
    Abstract: The disclosure generally relates to compositions and methods for the production of nucleic acid molecules. In some aspects, the invention allows for the microscale generation of nucleic acid molecules, optionally followed by assembly of these nucleic acid molecules into larger molecules. In some aspects, the invention allows for efficient production of nucleic acid molecules (e.g., large nucleic acid molecules such as genomes).
    Type: Application
    Filed: July 22, 2019
    Publication date: January 9, 2020
    Inventors: Thomas Poehmerer, Phillip Kuhn, Frank Notka, Andreas Zeidler, Korbinian Heil, Axel Trefzer, Geir Fonnum, Federico Katzen, Kristian Andersson, Xiquan Liang
  • Publication number: 20200010828
    Abstract: Antibody libraries comprising a plurality of heavy chain variable domains and/or a plurality of light chain variable domains, which comprise complementary determining regions (CDRs) found in naturally-occurring human antibodies, and methods of making such antibody libraries. The antibody libraries are free of members that comprise one or more liabilities affecting one or more features of such members. Further, the antibody libraries comprise members having heavy chain and/or light chain CDRs not found in the same naturally-occurring human antibody.
    Type: Application
    Filed: July 8, 2019
    Publication date: January 9, 2020
    Applicant: SPECIFICA INC.
    Inventors: Andrew Raymon Morton BRADBURY, Michael Frank ERASMUS, Andre TEIXEIRA
  • Publication number: 20200010829
    Abstract: Disclosed is a method for synthesizing cDNA by mixing a target RNA, a first oligonucleotide molecule, a second oligonucleotide molecule, and a reverse transcriptase, using the target RNA as a template, the first oligonucleotide molecule having a first region that hybridizes to the target RNA at 3? end, and having a second region that hybridizes to the second oligonucleotide molecule on 5? side of the first region.
    Type: Application
    Filed: May 29, 2019
    Publication date: January 9, 2020
    Applicant: SYSMEX CORPORATION
    Inventors: Kanako MASUMOTO, Yuki AIHARA
  • Publication number: 20200010830
    Abstract: Disclosed are compositions and methods for increasing virus production.
    Type: Application
    Filed: March 8, 2018
    Publication date: January 9, 2020
    Inventor: Ralph A Tripp
  • Publication number: 20200010831
    Abstract: The present invention relates to antisense oligonucleotides that are capable of modulating expression of Tau in a target cell. The oligonucleotides hybridize to MAPT mRNA. The present invention further relates to conjugates of the oligonucleotide and pharmaceutical compositions and methods for treatment of Tauopathies, Alzheimzer's disease, fronto-temporal dementia (FTD), FTDP-17, progressive supranuclear palsy (PSP), chronic traumatic encephalopathy (CTE), corticobasal ganglionic degeneration (CBD), epilepsy, Dravet syndrome, depression, seizure disorders and movement disorders.
    Type: Application
    Filed: July 3, 2019
    Publication date: January 9, 2020
    Applicant: Hoffmann-La Roche, Inc.
    Inventors: Peter HAGEDORN, Anja Mølhart HØG, Marianne L. JENSEN, Richard E. Olson
  • Publication number: 20200010832
    Abstract: Provided herein are neuroprotective molecules containing a sequence of 25-35 contiguous nucleotides between nucleotide 1 and nucleotide 50 of a mature human tRNA and at least four contiguous guanosine-containing nucleotides, where the sequence of 25-35 contiguous nucleotides contains a D-loop stem structure, the at least four contiguous guanosine-containing nucleotides are located at the 5? end of the neuroprotective molecule, and the neuroprotective molecule contains at least one deoxyribonucleotide. Also provided are neuroprotective molecules containing a sequence of 25-35 contiguous nucleotides between nucleotide 1 and nucleotide 50 of a mature human tRNACYS; and at least four contiguous guanosine-containing nucleotides, where the sequence of 25-35 contiguous nucleotides contains a D-loop stem structure and the at least four contiguous guanosine-containing nucleotides are located at the 5? end of the neuroprotective molecule.
    Type: Application
    Filed: September 20, 2019
    Publication date: January 9, 2020
    Inventors: Paul Anderson, Pavel Ivanov, Mohammed Emara
  • Publication number: 20200010833
    Abstract: A modified L-nucleic acid, containing an L-nucleic acid part conjugated to a non-L-nucleic acid part is described. The conjugate has extended retention time in and demonstrates a delayed elimination from an organism.
    Type: Application
    Filed: September 18, 2019
    Publication date: January 9, 2020
    Applicant: NOXXON Pharma AG
    Inventors: Christian Lange, Bernd Eschgfaller, Sven Klussmann
  • Publication number: 20200010834
    Abstract: The present invention provides engineered purine nucleoside phosphorylase (PNP) enzymes, polypeptides having PNP activity, and polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. Methods for producing PNP enzymes are also provided. The present invention further provides compositions comprising the PNP enzymes and methods of using the engineered PNP enzymes. The present invention finds particular use in the production of pharmaceutical compounds.
    Type: Application
    Filed: July 2, 2019
    Publication date: January 9, 2020
    Inventors: Scott J. Novick, Nikki Dellas, Vesna Mitchell, Da Duan, Jovana Nazor, Oscar Alvizo, Auric Anthony Sowell-Kantz, Jeffrey C. Moore, Mark Huffman, Agustina Rodriguez-Granillo, Deeptak Verma, Nicholas M. Marshall, Jay Russell, Keith A. Canada
  • Publication number: 20200010835
    Abstract: Provided herein are systems, compositions, and methods for the incorporation of unnatural amino acids into proteins via nonsense suppression or rare codon suppression. Nonsense codons and rare codons may be introduced into the coding sequence of a protein of interest using a CRISPR/Cas9-based nucleobase editor described herein. The nucleobase editors are able to be programmed by guide nucleotide sequences to edit the target codons in the coding sequence of the protein of interest. Also provided are application enabled by the technology described herein.
    Type: Application
    Filed: August 24, 2017
    Publication date: January 9, 2020
    Applicant: President and Fellows of Harvard College
    Inventors: Juan Pablo Maianti, David R. Liu, Luke W. Koblan
  • Publication number: 20200010836
    Abstract: The invention provides polynucleotide constructs for the regulation of gene expression by aptamer-based modulation of the accessibility of one or more polyadenylation signals and methods of using the constructs to regulate gene expression in response to the presence or absence of a ligand that binds the aptamer. The polynucleotide construct contains a riboswitch comprising an aptamer and an effector stem loop, wherein the effector stem loop comprises a polyadenylation signal sequence.
    Type: Application
    Filed: February 21, 2018
    Publication date: January 9, 2020
    Inventors: Xuecui Guo, Joonhee Han, Zhaojing Zhong