Abstract: Provided is a method that can efficiently produce a transgenic Taraxacum plant in a short period. A method of producing a transgenic Taraxacum plant, including: an infection step of infecting a tissue fragment from a Taraxacum plant with an Agrobacterium tumefaciens containing a plasmid containing a target gene or fragment thereof and hygromycin-resistance gene; a selective culture step of selecting the tissue fragment that has acquired the target gene from the tissue fragment obtained in the infection step by using hygromycin; a callus-inducing step of culturing the tissue fragment obtained in the selective culture step in a callus-inducing medium to form callus; a regeneration-inducing step of culturing the callus obtained in the callus-inducing step in a regeneration-inducing medium to form an adventitious embryo, an adventitious bud, and a shoot; and a rooting step of culturing the shoot obtained in the regeneration-inducing step in a rooting medium to root the shoot.

Abstract: The present invention provides methods for improving competency of plant cells for bacterial-mediated transformation comprising contacting the plant cells with an effective amount of polyethylene glycol (PEG) for a period of time prior to transformation. The ability to store and maintain competent plant cells for transformation and tissue culture allows more efficient planning and execution of large-scale experiments by providing flexibility of peak production hours, or during unplanned disruptions in the production process. These methods are useful in preserving the viability of plant cells in various storage conditions, thus improving their competency for transformation and tissue culture.

Abstract: The present invention relates to a method for changing the intercellular mobility of an min RNA of a gene in an organism, comprising: modifying a t RNA-like structure present in the m RNA by mutating the gene from which the m RNA is transcribed, or including the sequence of at RNA-like structure in the transcribed part of the gene. The method is in particular suited for plants. The intercellular mobility can be between different organs.

Abstract: Herein is disclosed synthetic oligonucleotides comprising 2?F-ANA nucleosides that can be utilized to control plant-chewing and phloem-feeding insects, bacteria present in such insects, and bacteria present in plants. The novel approaches and materials provided herein allow for reduction of pesticide and antibiotic use without the need to create genetically modified plants.

Abstract: The present invention relates to the development of expression cassettes to achieve tapetum specific reversible expression of transgene in female heterotic parent and in F1 progeny in the plants. The expression cassettes are based on the transcription regulation and light signaling. It includes two vector construct; female expression construct and male regulatory construct. The female expression construct was used to achieve tapetum specific expression of reporter gene while when cross is made with male plants having regulatory cassette, F1 plants were formed with abolished expression of reporter gene. Further, the system was deployed to achieve complete male sterility using candidate gene of male sterility; BECLIN1/ATG6 and successfully restoring the fertility of F1 by crossing with pollen of male regulatory transgenic. Here, we claim the expression system using which reversible male sterility can be achieved by expressing candidate gene for male sterility like BECLIN1 or other.

Abstract: The invention relates to nucleic acid sequences defining a genomic region conferring high silicon (Si) accumulation as discovered in the soybean (Glycine max) cultivar Hikmok sorip. Plants having this region, named HiSil, introduced in its nucleic acid exhibit increased Si uptake. Furthermore, markers associated with high Si accumulation and 5 methods of identifying high Si accumulating plants using the markers are provided. The method provided by the invention can be used to develop new plants with high Si accumulation capacity, through breeding, genetic modification or any other forms of plant propagation.

Abstract: Two novel cDNAs for two different genes, HDT1 and HDT2, are isolated from red clover and sequenced. Both HDT1 and HDT2 encode hydroxycinnamoyl-CoA:L-DOPA/tyrosine hydroxycinnamoyl transferase (HDT) which enzymatically produces clovamide and/or related hydroxycinnamoyl amides. Clovamide and related hydroxycinnamoyl amides reduce post-harvest protein degradation. Genetically altered alfalfa plants containing an expression cassette containing a cDNA encoding HDT1 or HDT2 are generated. These genetically altered alfalfa plants produce hydroxycinnamoyl-CoA:L-DOPA/tyrosine hydroxycinnamoyl transferase, which in turn produces clovamide and/or related hydroxycinnamoyl amides.

Abstract: The application describes producing polynucleotide variants of the AtHB 17 clade members and introducing the mutant variants into plants to improve plant traits. The mutant polynucleotides encode polypeptides that comprise mutations in the EAR motifs.

Abstract: This disclosure provides tobacco plants that exhibit altered photosynthesis as well as methods of making and using such plants.

Abstract: The present invention provides the specific expression promoters in rice and the application. The present invention applies the promoter in the plant genetic engineering. The sequence of the promoter provided in the present invention is composed of the nucleotide sequence shown in SEQ ID No. 11; the nucleotide sequence shown in SEQ ID No. 2; Or the nucleotide sequence shown in SEQ ID No. 3.

Abstract: Compositions, systems and methods are provided for conferring disease resistance to plant pathogens that use proteases to target plant substrate proteins inside plant cells. Briefly, the compositions, systems and methods are based upon plant substrate proteins that are targeted by pathogen-specific proteases and that activate nucleotide binding site-leucine rich repeat (NB-LRR) disease resistance proteins when cleaved by the protease. These substrate proteins are modified such that the endogenous protease recognition sequence is replaced by a protease recognition sequence specific to a different pathogen protease (i.e., a heterologous protease recognition sequence). The modified plant substrate protein therefore can be used in connection with its corresponding NB-LRR protein to activate resistance in response to cleavage by the heterologous pathogen-specific protease. When activated by the plant pathogen-specific protease, the pair initiates host defense responses thereto, including programmed cell death.

Abstract: Provided herein are carrot plants resistant to powdery mildew, and especially powdery mildew caused by the plant pathogen Erysiphe heraclei, wherein the powdery mildew resistance is provided by one or two dominant powdery mildew resistance genes. Also provided herein are molecular markers genetically linked to the present powdery mildew, and especially powdery mildew caused by the plant pathogen Erysiphe heraclei, resistance providing genes and the use thereof for identifying carrots plants, or Daucus carota plants, being resistant to powdery mildew, and especially powdery mildew caused by the plant pathogen Erysiphe heraclei. Also provided herein are seeds, plant parts, pollen, egg cells, callus, suspension culture, somatic embryos and edible plant parts of the present plants.

Abstract: Disclosed herein are chimpanzee adenoviral vectors that include neoantigen-encoding nucleic acid sequences derived from a tumor of a subject. Also disclosed are nucleotides, cells, and methods associated with the vectors including their use as vaccines.

Abstract: Methods of preparing papillomavirus nucleic acid transfer vectors, in-disassembled cluding by disassembly/reassembly of papillomavirus L1 and L2 virus-like particles, in a defined, cell-free high-efficiency production protocol. These methods may be used to efficiently encapsidate desired moieties, e.g., toxic or therapeutic nucleic acids such as DNA and RNA, and the resultant pseudovirus particles may be used as in vivo delivery vehicles.

Abstract: The present disclosure provides polynucleotide cassettes, expression vectors and methods for the expression of a gene in mammalian cells.

Abstract: The present invention relates to compositions and methods, in particular to methods based on systemic injection of rAAV, for delivering genes to cells of the central nervous system in mammals, such as brain neurons or glial cells, and in particular to motor neurons or glial cells of the spinal cord The invention also relates to methods of treating motor neuron disorders in mammals by expression of therapeutic genes. The invention stems from the unexpected discovery that peripheral injection of AAV vectors leads to a bypass of the blood brain barrier and a massive infection of motor neurons. The invention may be used in any mammal, including human subjects.

Abstract: A method is provided for integrating a DNA fragment of a desired base sequence into a site located adjacent to a binding region of a DNA-binding protein bound to a DNA molecule, the method including bringing the DNA fragment having a base sequence including a transposase-binding sequence and the desired base sequence close to the binding region using a specific binding substance to the DNA-binding protein, binding transposase to the transposase-binding sequence, and activating the transposase such that the DNA fragment of the desired base sequence is integrated into the site located adjacent to the binding region.

Abstract: Described herein are methods for treating disorders affecting ocular and non-ocular tissue, such as corneal dystrophies and microsatellite expansion diseases. The methods use a nuclease system, such as Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR associated (Cas) 9 (CRISPR-Cas9), to cut and/or repair genomic DNA. Such methods may further comprise a DNA double-stranded break (DSB) repair system comprising a repair template in combination with a Non-Homologous End-Joining (NHEJ) or Homology Directed Repair (HDR) targeted to the one or more CRISPR-Cas9 cleavage sites.

Abstract: The present invention relates to a long-chain dibasic acid with low content of hydroxyl acid impurity and a production method thereof, in particular to a method for producing a long-chain dibasic acid with low content of hydroxyl acid impurity by fermenting a long-chain dibasic acid producing strain prepared by homologous recombination method. The present invention relates to a recombinant long-chain dibasic acid producing microorganism, having increased alcohol dehydrogenase activity and optionally decreased acetyl-CoA oxidase activity. The present invention also relates to a method of producing a long-chain dibasic acid by the recombinant long-chain dibasic acid producing microorganism and use thereof.

Abstract: Provided is a method for altering a targeted site of a DNA in a cell, including a step of stimulating the cell with a factor inducing a DNA modifying enzyme endogenous to the cell, and bringing a complex of a nucleic acid sequence-recognizing module specifically binding to a target nucleotide sequence in a given DNA and a DNA modifying enzyme-binding module bonded to each other into contact with the DNA to convert one or more nucleotides in the targeted site to other one or more nucleotides or delete one or more nucleotides, or insert one or more nucleotides into the targeted site.

Abstract: The present disclosure relates generally to the production of tellurium and selenium nanostructures in bacteria. The nanostructures are unique in size, shape, length and stability.

Abstract: Described is a method for the production isoamyl alcohol (3-methylbutan-1-ol) comprising the enzymatic conversion of 3-methylbutyryl-CoA (isovaleryl-CoA) into isoamyl alcohol comprising: (a) two enzymatic steps comprising (i) first the enzymatic conversion of 3-methylbutyryl-CoA into 3-methylbutyraldehyde (3-methylbutanal or isovaleraldehyde); and (ii) then enzymatically converting the thus obtained 3-methylbutyraldehyde into said isoamyl alcohol; or (b) a single enzymatic reaction in which 3-methylbutyryl-CoA is directly converted into isoamyl alcohol by making use of an alcohol-forming short chain acyl-CoA dehydrogenase/fatty acyl-CoA reductase or an alcohol-forming fatty acyl-CoA reductase (long-chain acyl-CoA:NADPH reductase) (EC 1.2.1.84). Further, described is the above method wherein the 3-methylbutyryl-CoA can be provided by the enzymatic conversion of 3-methylcrotonyl-CoA into said 3-methylbutyryl-CoA.

Abstract: The present invention relates to a long-chain dibasic acid with low content of long-chain dibasic acid impurity of shorter carbon chain, to the preparation of a long-chain dibasic acid producing strain by directed evolution of POX gene and homologous recombination, and to the production of a long-chain dibasic acid with low content of long-chain dibasic acid impurity of shorter carbon chain by using the strain. The present invention also relates to a strain containing a mutated promoter, wherein, when a long-chain dibasic acid is produced by fermentation of this strain, the content of the acid impurity of shorter carbon chain in the fermentation product is significantly reduced.

Abstract: This invention relates to a process for preparing succinate ester from a succinic acid salt present in a fermentation broth. In the first stage of this invention, renewable carbon resources are utilized to produce succinic acid in the form of a succinic acid salt through biological fermentation. The succinic acid salt present in the fermentation broth is subjected to double displacement reaction with a strong acid leading to the release of succinic acid. Succinic acid is recovered by fractional crystallization integrated with an alcohol washing step and subjected to esterification reaction to produce succinate ester which is purified by fractional distillation. The succinate ester thus obtained is converted into 1,4-butanediol, gamma-butyrolactone and tetrahydrofuran through hydrogenation reactions. The succinate ester can also be hydrolyzed to yield highly pure succinic acid.

Abstract: A process for producing at least one polyhydroxyalkanoate through gas fermentation may include: providing at least one gas fermentation vessel having a volume partially filled with a liquid fermentation broth that includes: water, suspended gas-fermenting microorganisms capable of producing the at least one polyhydroxyalkanoate, and nutrients for the gas-fermenting microorganisms, a remaining part of the volume of the at least one gas fermentation vessel being filled with a gas phase; continuously withdrawing an aliquot of the liquid fermentation broth from the at least one gas fermentation vessel; supplying a gaseous substrate comprising CO2, H2, and O2; cultivating the gas-fermenting microorganisms to form a cell mass containing the at least one polyhydroxyalkanoate; and recovering the at least one polyhydroxyalkanoate from the cell mass.

Abstract: The invention relates to a long-chain dibasic acid with low content of fatty acid impurity and a method of producing it, in particular to the preparation of a long-chain dibasic acid producing strain by using directed evolution and homologous recombination, and to the fermentation production of a long-chain dibasic acid with low content of fatty acid impurity by using said strain. The invention relates to an isolated mutated CPR-b gene, homologous gene or variant thereof, relative to the GenBank Accession Number AY823228, taking the first base upstream of the start codon ATG as ?1, comprising a base mutation ?322G>A, and taking the first base downstream of the stop codon TAG as 1, comprising mutations 3TUTR.19C>T and 3?UTR.76_77insT.

Abstract: The invention relates to a long-chain dibasic acid with low content of monobasic acid impurity and a production method thereof, in particular to the preparation of a long-chain dibasic acid producing strain by means of directed evolution and homologous recombination, and to the production of a long-chain dibasic acid with low content of monobasic acid impurity by fermentation of said strain. The invention relates to a mutated CYP52A12 gene, homologous gene or variant thereof, which, relative to GenBank Accession Number AY230498 and taking the first base upstream of the start codon ATG as ?1, comprises a mutation. The invention relates to a strain comprising said mutated CYP52A12 gene, homologous gene or variant thereof wherein when the strain is fermented to produce a long-chain dibasic acid, the content of monobasic acid impurity in the fermentation product is significantly reduced.

Abstract: The present application discloses a method for producing piperonal by using a recombinant engineered bacterium co-expressing trans-anethole oxygenase and formate dehydrogenase, and an engineered bacterium thereof, including constructing a formate dehydrogenase gene fdh and trans-anethole oxygenase gene tao or trans-anethole oxygenase mutant gene co-expression recombinant vector; inductively expressing recombinant genetically engineered bacterium; and producing piperonal by using the recombinant genetically engineered bacterium. 15.91 g/L of piperonal with a transformation rate of 79.55% and a time-space transformation rate of 2.27 g/L/h can be finally obtained during catalysis, and the yield is significantly improved compared with the existing piperonal, thereby being more conducive to the smooth realization of industrial production.

Abstract: The subject invention concerns materials and methods for alkene reduction of compounds, such as levoglucosenone (LGO) and (,S)-Y-hydroxymethyl-a,P-butenolide (HBO), using an alkene reductase enzyme. In one embodiment, a method of the invention comprises alkene reduction of a target compound by reacting the compound with an Old Yellow Enzyme (OYE) that reduces alkene bonds. In one embodiment, the OYE is OYE 2.6 from Pichia stipites and comprises the amino acid sequence of SEQ ID NO: 1. In a specific embodiment, the enzyme is an Old Yellow Enzyme (OYE) 2.6 mutant having an amino acid substitution at position 78 in the sequence, wherein the tyrosine at position 78 is substituted with a tryptophan amino acid (Y78W) and is designated as OYE 2.6 Y78W (SEQ ID NO:2).

Abstract: The application relates to the production of RNA of interest, more specifically of messenger RNA of interest or of long non-coding RNA of interest, by yeasts with recombinant pseudo-viral particles. The recombinant yeasts have been genetically modified in order to produce the RNA of interest in the form of a complex, particularly in the form of recombinant pseudo-viral particles. These recombinant pseudo-viral particles are produced from certain elements of yeast Ty retrotransposon, but do not retrotranscribe the RNA that they contain. Thus, the application relates to the components that are thus capable of being implemented or produced, and particularly to the nucleic acid constructs, kits, bacteria cells, yeast cells, culture or transfection media containing them, as well as to a method for producing a pharmaceutical composition, particularly for medical applications, more specifically for vaccines, anti-tumour and pro-regenerative applications.

Abstract: A method of homologous recombination, including in vitro joining two or more target nucleic acid molecules with a first exonuclease, and recombining the two or more target nucleic acid molecules in the presence of a second exonuclease and an annealing protein. The recombined target nucleic acid molecules share at least one homologous sequence.

Abstract: The present invention provides engineered deoxyribose-phosphate aldolase polypeptides useful under industrial process conditions for the production of pharmaceutical and fine chemical compounds.

Abstract: The present invention provides engineered galactose oxidase (GOase) enzymes, polypeptides having GOase activity, and polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. Methods for producing GOase enzymes are also provided. The present invention further provides compositions comprising the GOase enzymes and methods of using the engineered GOase enzymes. The present invention finds particular use in the production of pharmaceutical and other compounds.

Abstract: Devices, methods and systems are for identifying the cell type of an unknown microorganism. The device includes: an apparatus for culturing unknown organism(s), a diagnostic data acquisition tool and a computer program. The method includes: incubation of the sample with a growth medium (with or without toxins), and an analysis of the metabolites detected in the sample. The computer system compares the results collected from the device to reference metabolite profiles.

Abstract: The present invention relates to a method, a medium and a kit for the enrichment and detection of Listeria species, especially Listeria monocytogenes. The medium is an enrichment medium comprising C12 to C16 fatty acids and/or derivatives thereof.

Abstract: A microfluidic processing device includes a substrate defining a microfluidic network. The substrate is in thermal communication with a plurality of N independently controllable components and a plurality of input output contacts for connecting the substrate to an external controller. Each component has at least two terminals. Each terminal is in electrical communication with at least one contact. The number of contacts required to independently control the N components is substantially less than the total number of terminals. Upon actuation, the components typically heat a portion of the microfluidic network and/or sense a temperature thereof.

Abstract: A droplet generating method includes: providing a micro-pipe for dispensing a first liquid and a container containing a second liquid; providing a moving and locating device for positioning the micro-pipe over the container; providing a liquid driving device connecting to the micro-pipe through a connecting tube; providing a vibrating equipment connected to the micro-pipe for vibrating the micro-pipe; forming a relative periodic vibration between the micro-pipe and the container so that the outlet end of the micro-pipe is displaced to touch the second liquid in the container during a relative periodic vibration; and dispensing the first liquid in the micro-pipe out from the outlet end of the micro-pipe during the relative periodic vibration to generate a plurality of droplets of the first liquid in the second liquid which is induced by a force of the second liquid imposed on the first liquid at the outlet end.

Abstract: Described are methods for identifying antibiotic resistant bacteria, quantifying bacteria growth, and applying an antibiotic susceptibility test (AST) in one or more biological samples containing a bacteria and chips used in these methods.

Abstract: The invention comprises a novel method and compositions for sequencing library preparation, which increases the throughput of single-molecule sequencing (SMS) platforms by generating long concatenated templates from pools of short DNA molecules.

Abstract: The present disclosure provides methods and compositions for the multiplexed detection of multiple analytes from a sample. Analytes may be nucleic acid analytes. Detection of analytes may comprise contacting one or more sample subsets with hybridization probes, thereby generating one or more cumulative signal measurements capable of detecting the presence of absence of a plurality of analytes.

Abstract: Time-resolved nucleic acids include a long-lifetime FRET donor with an emission lifetime of at least one millisecond (such as a terbium complex), configured as a donor in a first FRET process, and at least one fluorescent dye with an emission lifetime of less than 100 nanoseconds configured as an acceptor in the FRET process. They can be configured as photonic wires, hybridization probes or beacons, and/or systems for computing logical operations.

Abstract: The present disclosure provides methods for detecting a single-stranded target RNA. The present disclosure provides methods of cleaving a precursor C2c2 guide RNA array into two or more C2c2 guide RNAs. The present disclosure provides a kit for detecting a target RNA in a sample.

Abstract: The present disclosure provides methods for detecting a single-stranded target RNA. The present disclosure provides methods of cleaving a precursor C2c2 guide RNA array into two or more C2c2 guide RNAs. The present disclosure provides a kit for detecting a target RNA in a sample.

Abstract: A method is provided for probabilistically assigning a tissue of origin to a nucleic acid in a sample, e.g., DNA in a cell-free fluid sample obtained from a human subject. A hydroxymethylation profile is generated for the sample DNA and then compared across a reference data set of hydroxymethylation profile vectors, where each hydroxymethylation profile vector identifies the hydroxymethylation profile at a specific reference locus, the tissue-specific gene associated with the reference locus, and the tissue with which the gene and reference locus are associated. A tissue of origin can be probabilistically assigned to the sample nucleic acid using the results of the comparison. Other methods of use are also provided.

Abstract: The present invention concerns a method of identifying a patient that is likely to be responsive to treatment with a protein arginine N-methyltransferase 5 (PRMT5) inhibitor comprising: evaluating a biological sample from the patient for the presence of a spliceosome alteration, wherein the presence of any said alteration indicates a higher likelihood for said patient to be responsive to treatment with said PRMT5 inhibitor than in the absence of any said mutation or alteration.

Abstract: The invention provides methods for amplifying nucleic acids, particularly methods for reducing density-dependent GC bias and for reducing nucleic acid damage in a bridge amplification of a nucleic acid template. The invention also provides methods for evaluating the effect of reagents and/or additives on nucleic acid damage during bridge amplification of nucleic acid template strands. The methods are suited to solid phase amplification, for example, utilizing flow cells.

Abstract: In some aspects, the present disclosure provides methods for enriching amplicons, or amplification products, comprising a concatemer of at least two or more copies of a target polynucleotide. In some embodiments, a method comprises sequencing the amplicons comprising at least two or more copies of a target polynucleotide. In some embodiments, the target polynucleotides comprise sequences resulting from chromosome rearrangement, including but not limited to point mutations, single nucleotide polymorphisms, insertions, deletions, and translocations including fusion genes. In some aspects, the present disclosure provides compositions and reaction mixtures useful in the described methods.

Abstract: In some aspects, the present disclosure provides methods for identifying sequence variants, as well as methods of determining copy number of a genetic locus in a sample. Systems and kits for performing methods of the disclosure, as well as compositions produced by or useful in methods of the disclosure are also provided. In some embodiments, methods comprise extending 3? ends of polynucleotides by adding one or more pre-determined nucleotides. In some embodiments, methods comprise use of a strand-tagging sequence.

Abstract: A method for determining the presence of an allele, including (a) binding a polymerase to a double stranded nucleic acid that includes a primer hybridized to a template, the template including a first allele of a locus; (b) adding a nucleotide to the primer via catalytic activity of the polymerase, thereby producing an extended nucleic acid; (c) dissociating the polymerase from the extended nucleic acid; (d) detecting dissociation of the polymerase from the extended nucleic acid; and (e) comparing the dissociation of the polymerase from the extended nucleic acid to dissociation of the polymerase from a second double stranded nucleic acid, the second double stranded nucleic acid including a primer hybridized to the same position of the locus as the primer of the extended nucleic acid.

Abstract: The invention is directed to methods and apparatus for detecting sequences of optical signals from parallel reactions on an array of nanostructures, such as nanopores, nanowells, or nanoparticles. In accordance with the invention, an array of nanostructures is provided, each nanostructure comprising a reaction site and each capable of confining a reaction that generates a sequence of optical signals, and the nanostructures of the array being arranged in clusters each comprising a number of nanostructures. Each different cluster is disposed within a different resolution limited area and the number of nanostructures in each cluster is either greater than one or a random variable with an average value greater than zero. Optical signals from reactions in the nanostructures are detected by an optical system operatively associated with the array.