Abstract: An electrochemical sensor (1, 101) has a sensor element (15, 115) with a measuring surface (8, 108) that faces a measuring medium (5) during use. The sensor element has a planar measuring element (2, 102). A sensor shaft (4, 104) has an aperture (13, 113) with a bezel (11, 111) at an end which, during use, faces the measuring medium. The sensor element is installed in the area of the aperture. The electrochemical sensor also has an annular sealing element (9, 109), which is arranged between the sensor element and the bezel. An insulator element (10, 110) is firmly and inseparably connected to the measuring element, exposing or recessing the measuring surface. Thus, the sealing element, which protects the electrochemical sensor against the ingress of measuring medium, is arranged sealingly between the insulator element and the bezel.

Abstract: Voltammetric sensors prepared from composite materials and optionally using microfabrication techniques enable detection of analyte in sample volumes under ten microliters.

Abstract: In general, a liquid phase ion mobility spectrometer is described in which ions in a drift chamber are pushed against an increasing retarding electric field by means of liquid flow. In some examples, a method comprises ionizing an analyte to generate ions; introducing the ions into a separation region of a drift chamber; directing, at a velocity, a liquid flow through the separation region to apply first forces to the ions within the separation region of the drift chamber; using an electric field with a non-zero gradient, applying second forces to the ions within a separation region of a drift chamber, the second forces counter the first forces; modifying at least one of the velocity or the electric field; and after modifying at least one of the velocity or the electric field, detecting the ions at an ion detector to generate a signal.

Abstract: A method for inspecting a test object for defects by using an ultrasonic probe including a transmitter, a wedge, and a receiver, the method comprising: placing the ultrasonic probe on the test object; transmitting ultrasound from the transmitter into the wedge such that surface waves propagate along a surface area of the test object; and determining that there is a defect in a part of the test object overlapped with the wedge when the receiver receives an ultrasonic echo, after the transmission of the ultrasound by the transmitter, in a time shorter than a time required to receive a front end-reflected ultrasonic echo produced by reflection of the surface waves at a front end of the wedge.

Abstract: This present disclosure relates to sensors capable of sensing mass, stiffness, and chemical or biological substances. More specifically, this disclosure provides the design and implementation of a piecewise-linear resonator realized via diode- and integrated circuit-based feedback electronics and a quartz crystal resonator. The proposed system is fabricated and characterized, and the creation and selective placement of the bifurcation points of the overall electromechanical system is demonstrated by tuning the circuit gains. The demonstrated circuit operates around at least 1 MHz.

Abstract: A variable value calculating process includes: measuring a propagation time of the propagation of an ultrasound wave through a measurement sector inside a housing; obtaining a temperature calculated value on the basis of the measured value of the propagation time and a reference distance for the measurement sector; obtaining a temperature measured value by measuring the temperature inside the housing; and obtaining a temperature replacement fluctuation value indicating a difference between the temperature calculated value and the temperature measured value. The variable value calculating process is executed for each of a plurality of temperature conditions under which the temperature of a reference gas inside the housing differs. A temperature compensation table in which the temperature of a gas to be measured is associated with a temperature compensation value relating to the temperature is obtained on the basis of the temperature replacement fluctuation values obtained under each temperature condition.

Abstract: Described is a fluidic manifold that includes a block formed of multiple layers each bonded to at least one adjacent layer at a layer interface. Bonding may be achieved using a diffusion bonding process. The block includes one or more attachment surfaces and at least two fluidic channels. Each fluidic channel is at least partially disposed at one of the layer interfaces and has a first end at one of the attachment surfaces. Each attachment surface includes an attachment feature at the first end of one of the fluidic channels to enable a fluidic coupling of the two fluidic channels through a fluidic component. Attachment features include, for example, a compression fitting coupling body adapted to receive a conventional seal, such as a ferrule and compression screw, and a fitting body that permits a face seal or gasket seal between the fluidic component and the block.

Abstract: Described are stator arrays for multi-valve systems used in different chromatographic applications. Also described is a mounting assembly for a multi-valve system that includes the stator array. Each stator array has a stator body having a first stator surface and a second stator surface. Each stator surface has a plurality of stator ports and is configured to engage a rotor surface of a rotary valve actuator. A fluid channel inside the stator body couples one of the stator ports of the first stator surface to one of the stator ports of the second stator surface. The fluid channel may be a microfluidic channel. A solid-state diffusion bonding process in which two or more parallel layers of material are joined together may be used to fabricate the stator body. Complex fluid channel networks may be formed using many fluid channels formed in multiple layers within the stator body.

Abstract: A sample injector is disclosed comprising a switching valve comprising an input port, an aspiration/dispensing port, a loop input port and a loop output port, a pump port and a column port, an aspiration pump connected to the aspiration/dispensing port via a buffer loop for aspirating a sample into the buffer loop when the aspiration/dispensing port is connected to the loop input port, a sample loop connected to the switching valve between the loop input port and the loop output port, for receiving the sample aspirated into the buffer loop when the loop input port is connected to the aspiration/dispensing port, a column connected to the column port, and a pump connected to the pump port, for injecting the sample received into the sample loop into the column when the pump port is connected to the loop output port and the column port is connected to the loop input port.

Abstract: A fraction collector controller includes a threshold storing part, a peak detecting part, a number-of-points-of-data setting part, and a control part. The peak detecting part retrieves, at regular intervals, signals that are from a detector of a liquid chromatograph and are used as points of data, and detects a start point and an end point of a peak by comparing a signal strength and/or an inclination of each of the points of data with the threshold(s). The number-of-points-of-data setting part is configured to set a number of the points of data with which the peak detecting part compares the threshold(s) to detect a start point and an end point of a peak.

Abstract: A variable fluidic restrictor of a liquid chromatography system including a stator body, the stator body include a plurality of fluidic channels located within the stator body, wherein each fluidic channel of the plurality of fluidic channels includes a restrictor element, wherein a flow of a fluid through the variable fluidic restrictor is selectively restricted based on a position of an external element coupled to the stator body is provided. Furthermore, an associated method is also provided.

Abstract: The present disclosure relates to a test device for the pH-dependent detection of urine by the combined immobilization on the cellulose-containing support matrix of the pH indicator dye and the acid/base reacted with it. In addition, the present disclosure relates to a sanitary article comprising the test device according to the present disclosure, and a process for preparing the test device according to the present disclosure.

Abstract: This patent discloses methods, systems and computer program product for locally or remotely requesting, displaying and communicating real time vehicle pollution or air quality index determination of vehicles in operation, from a mobile device or non mobile device such as a smart phone or tablet; or non mobile device such as a computer; and communicating over a data network with other vehicles or transport systems or traffic management systems or devices for measuring, monitoring and controlling of vehicle pollution and air quality index parameters.

Abstract: A gas sensing device and a gas monitoring system for sensing ground gas is disclosed. The device comprises a power source, data processing means and memory means operably interfaced with the data processing means, which store sampling configuration data and sensed data. The device further comprises sensing means operably interfaced with the power source and the data processing means. The sensing means samples ambient gas adjacent the device according to first sampling configuration data and outputs sensed gas concentration data and sensed gas flow data. The device further comprises a telemetry module operably interfaced with the power source and the data processing means. The telemetry module communicates the sensed data to a remote terminal and receives second sampling configuration data from the remote terminal for updating the first sampling configuration data. The system comprises the terminal and at least one gas sensing device remote from the terminal.

Abstract: An analysis unit includes a frontal plane to access the analysis unit, a mounting plane perpendicular to the frontal plane, and a gas measurement device. The gas measurement device includes a connection housing part and a main housing part. The connection housing part includes an inlet channel and an outlet channel which extend from the mounting plane to a first connection plane. The main housing part includes a second connection plane which corresponds to the first connection plane. The main housing part rests on the first connection plane. The main housing part further includes an inflow channel which fluidically communicates with the inlet channel via the first connection plane, and a recirculation channel which fluidically communicates with the outlet channel via the first connection plane. The first and second connection planes are tilted at an angle of 45° from the mounting plane in a direction of the frontal plane.

Abstract: A nanomaterial-based gas sensor system comprising a low voltage circuitry which includes a transducer to detect changes in electrical properties of a multi-channel gas sensor array, analog signal conditioning, and an A/D conversion to provide a signal to a digital back-end.

Abstract: An electronic device can comprise: a sensor module; and a processor for acquiring periphery state information indicating information on the outside of the electronic device and/or device state information indicating information on the inside of the electronic device, acquiring a measurement profile including information regarding target gas to be measured and a detection period of the target gas on the basis of the periphery state information and/or the device state information, and detecting the target gas through the sensor module according to the measurement profile.

Abstract: A nanomaterial-based gas sensor system comprising a specific Platinum (Pt) patterned electrode structure is built on a Silicon (Si)/Silicon Dioxide (SiO2) substrate. The pattern is compatible with high volume manufacturing and nano gas sensor development requirements.

Abstract: A process for making highly sensitive nano-nucleated structures for use in room temperature nanohybrid gas sensors which utilize high surface area nanomaterials (carbon nanotubes, dichalcogenides, graphene, metal-organic frameworks, metal oxides, etc.) functionalized with atomically dispersed metal catalysts for sensing airborne environmental pollutants.

Abstract: The present disclosure relates to a measuring arrangement for measuring an ozone content in a measured medium, including: a first sensor surface and a second sensor surface; a first cover element adjacent the first sensor surface and including an ozone binder that binds ozone without releasing oxygen or any species further reacting to form oxygen; a second cover element adjacent the second sensor surface and including an ozone converter that reacts with ozone to form oxygen; a measuring sensor configured to generate a first measurement signal dependent on the oxygen concentration at the first sensor surface and a second measurement signal dependent on the oxygen concentration at the second sensor surface; and an electronic evaluation unit configured to determine the ozone content in the measured medium based on the first and the second measurement signals.

Abstract: A slag volume evaluation method for a molten metal surface includes calculating an approximation curve indicating a correspondence between a thickness of slag and a density parameter in advance by measuring thicknesses of a plurality of pieces of the slag which float on a surface of a molten metal in a container and differ from each other in thickness, and calculating a value of the density parameter which is correlated to a density in a pixel region corresponding to the plurality of pieces of the slag in a captured image of a molten metal surface in the container; and calculating a volume of the slag by calculating and integrating the thickness of the slag for each of pixels constituting the captured image obtained by capturing an image of the molten metal surface which is an evaluation target, according to a value of the density parameter of each of the pixels and the approximation curve.

Abstract: An electrical stimulation and monitoring device that includes multiple signal paths that are connected in parallel with each other, and each containing a stimulation or sensing electrode, a DC-blocking capacitor and a stimulation or sensing channel. A semiconductor substrate provided for hosting the DC-blocking capacitors is connected electrically to a DC voltage source through a substrate holding capacitor. Such substrate holding capacitor reduces a blanking time between stimulation and sensing periods, and also reduces cross-couplings between different ones of the signal paths while all the DC-blocking capacitors are provided on one and same semiconductor substrate.

Abstract: Systems and methods for controlling molecular translocation in solid-state nanopores by edge field leakage. The system dramatically reduces (by orders of magnitude) and controls the fast electrophoretic velocity of molecules to realize sensitive and selective solid-state nanopore sensors for polynucleotides and sequencing platforms.

Abstract: A device for separating blood plasma from whole blood includes a first reservoir and a second reservoir. The first reservoir is configured to receive a sample of whole blood including red blood cells and includes a collection region and a constricted region. The second reservoir is fluidically connected to the constricted region of the first reservoir, such that, responsive to centrifugal force applied to the device, the sample of whole blood disposed within the first reservoir separates into a first fraction and a second fraction. The first fraction is located in the collection region and includes blood plasma from which substantially all red blood cells have been removed. The second fraction is located in the second reservoir and includes blood plasma and red blood cells that have been removed from the first fraction by the centrifugal force. The constricted region inhibits the second fraction from entering the collection region.

Abstract: Described herein are methods of detecting levels hydrogen sulfide (H2S) to diagnose H2S positive disease or conditions. Examples of H2S positive diseases and conditions include small intestinal bacterial overgrowth, diarrhea, fatigue, bowel urgency and abdominal pain. The H2S level can guide treatments for subjects who have high levels of H2S.

Abstract: An exhaled gas detector includes: a gas detection element configured to measure a specific gas; and an analysis unit configured to analyze a signal output from the gas detection element. The gas detection element measures the specific gas in an exhalation of non-drinking time and generates a first reference signal value. The analysis unit calculates a first threshold value on the basis of the first reference signal value. The gas detection element measures the specific gas in an inspection gas and generates a first measurement signal value. The analysis unit determines that the inspection gas is identified with an exhalation of a person by determining that the first measurement signal value has exceeded the first threshold value.

Abstract: Disclosed are: a method for activating a helper T cell, which comprises the step of adding a WT1 peptide to an antigen-presenting cell to activate the helper T cell, wherein the WT1 peptide is capable of binding to any one selected from an HLA-DRB1*1501 molecule, an HLA-DPB1*0901 molecule and an HLA-DPB1*0501 molecule; a composition for use in the method; a therapeutic and/or prophylactic method for cancer by activating a helper T cell; a pharmaceutical composition for use in the therapeutic and/or prophylactic method; and others.

Abstract: The present disclosure relates to an Extra Cellular Matrix composition specific for cancer type and a tumor microenvironment platform for long term culturing of tumor tissue, wherein said culturing provides human ligands and tumor tissue micro-environment to mimic physiologically relevant signalling systems. The present disclosure further relates to the development of a Clinical Response Predictor and its application in the prognostic field (selection of treatment option for the patient) and translational biology field (development of anticancer drugs). The disclosure further relates to a method of predicting clinical response of a tumor patient to drug(s). The disclosure further relates to a method for screening tumor cells for the presence of specific markers for determining the viability of said cells for indication of tumor status.

Abstract: An in silico screening method generated compounds that are against laminin receptor 37LR and their anti-cancer functions in prostate cancer cell lines are disclosed herein. A group of derivatives based on the hit compound from the in silico screening are synthesized and tested with improved IC50 value that can have relevant clinical use for prostate cancer or osteoarthritis.

Abstract: Systems and methods relate to transgenic organisms and their use as biosensors are described. In some embodiments, the systems and methods include a first population of transgenic organisms that includes a first constitutively expressed reporter gene, and a first transgene that includes a first inducible promoter from a response pathway gene, wherein the first inducible promoter is coupled to a first reporter gene. Other embodiments are described.

Abstract: A testing system includes a well cover portion, a sensor portion extending from the well cover portion, a sensing surface disposed on the sensor portion, a conducting wire extending through the sensor portion and contacting the sensing surface, a transducer connected to the conducting wire, and a reference electrode extending through the well cover portion.

Abstract: Methods are described herein for screening an antibody producing cell within a microfluidic environment. The antibody producing cell may be a B cell lymphocyte, which may be a memory B cell or a plasma cell. An antigen of interest may be brought into proximity with the antibody producing cell and binding of the antigen by an antibody produced by the antibody producing cell may be monitored. Methods of obtaining a sequencing library from an antibody producing cell are also described.

Abstract: A method for measuring kinetics of Ca2+ flux in differentially responding T cells that form monolayer on the glass surface in response to antigenic peptides or live target cells comprising: immobilizing T cells labeled with Ca2+ sensitive fluorophore on the glass bottom of a well, covered with capturing antibody or a capturing protein that bind to non-stimulatory T-cell surface receptor; adding to the well a single or multiple peptide epitopes that binds to the cell surface MHC molecules to be presented for recognition by cognate T cells; the stimulatory signal could also be delivered by live target cells that display peptide epitope(s); wherein the recognition of stimulatory of pMHC by the peptide specific T cells leads to increase of intracellular Ca2+ level and fluorescence intensity in the responding T cells.

Abstract: Methods of minimizing hook effect interference in an immunoassay are disclosed. Also disclosed are reagents, kits, and immunoassay devices that may be utilized in accordance with the method.

Abstract: The present disclosure provides, inter alia, methods and compositions (e.g., conjugates) for imaging, at high spatial resolution, targets of interest.

Abstract: The invention relates to methods of detecting a target antigen by optical detection, isotopic detection, or detection by electron microscopy, comprising contacting a first or second antibody with at least one monovalent antibody that specifically binds said first or second antibody, wherein the monovalent antibody has one or more fluorescent label(s), chromophore label(s), isotope label(s), or metal label(s) attached to it. The invention also relates to complexes formed by the methods of the invention, kits for performing the method of the invention, monovalent antibodies useful for conducting the methods of the invention, as well as uses of the monovalent antibody according to the methods of the invention.

Abstract: A process for monitoring binding events, a coating compound, and a device for monitoring binding events are disclosed. The process includes providing two reservoirs that contain an electrically conductive fluid, wherein the reservoirs are separated by a membrane having a nanopore. The process also includes selecting a target compound, selecting a ligand based on the selection of the target compound, and preparing a coating that includes the coating compound, wherein the coating compound includes the ligand and a moiety that immobilizes the ligand. Additionally, the process includes applying the coating to the inner surface of the nanopore, adding the target compound to a conductive liquid in the first chamber, establishing a voltage gradient across the membrane, and electrically monitoring translocation of the target molecule. The device includes the reservoirs and the membrane having the coated nanopore.

Abstract: A sealed lateral flow device includes a test strip, a base structure, and a removable cover fixed to the base structure. The test strip includes one or more of a sample application zone, a detection zone, and a liquid collection zone. The test strip is disposed within a bottom section of the base structure. The removable cover fixed to the base structure sealingly encloses the test strip within the base structure and forms an airtight seal of the base structure.

Abstract: The present invention relates to the use of at least two of 2-Cys-PRDXs selected from a group consisting of PRDX1, PRDX2, PRDX3, PRDX4 and PRDX5 as a biomarker to diagnose, differentiate and/or assess the risk of chronic kidney disease (CKD). The present invention also relates to a method of diagnosis and a method of determining an increased risk of chronic kidney disease (CKD). A kit suitable for diagnosis and differentiation of chronic kidney disease (CKD) selected from the group comprising lupus nephritis (LN), IgA nephropathy (IgAN) and autosomal-dominant polycystic kidney disease (ADPKD) in a subject is also provided.

Abstract: The disclosed embodiments concern non-invasive methods, and apparatus, and systems for identifying infections. The methods are predicated on identifying discriminating peptides present on a peptide array, which are differentially bound by the different mixtures of antibodies present in samples from subjects consequent to an infection relative to binding of mixtures of antibodies present in reference subjects.

Abstract: The present invention relates to a novel MMP-8 activation product such as a MMP-8 middle-part activation product. The invention also relates to detecting such a MMP-8 activation product or activated MMP-8 fragments in a biological sample derived from a subject and to the use thereof for diagnosing diseases which relate to abnormal or elevated levels of activated MMP-8.

Abstract: Methods and compositions are provided for assessing (e.g., diagnosing), treating, and preventing diseases, especially cancer, and particular lung cancer, using lung cancer markers (LCM). Individual LCM and panels comprising multiple LCM are provided for these and other uses. Methods and compositions are also provided for determining or predicting the effectiveness of a treatment or for selecting a treatment using LCM. Methods and compositions are further provided for modulating cell function using LCM. Also provided are compositions that modulate LCM (e.g., antagonists or agonists), such as antibodies, proteins, small molecule compounds, and nucleic acid agents (e.g., RNAi and antisense agents), as well as pharmaceutical compositions thereof. Further provided are methods of screening for agents that modulate LCM, and agents identified by these screening methods.

Abstract: The present invention relates to methods for determining the propensity of cells to interact in a population of cells comprising at least two distinguishable subpopulations of cells. In addition, the present invention provides methods for diagnosing a disease or predisposition to a disease of a cell donor, wherein the method comprises the determination of the propensity of cells to interact in a population of cells obtained from the donor, wherein the population of cells comprises at least two distinguishable subpopulations of cells. The invention also provides methods for determining whether a subject suffering from or predisposed to a disease will respond or is responsive to treatment with a therapeutic agent by determining the alteration of the propensity of cells to interact in a population of ceils obtained from the subject, wherein the population of cells comprises at least two distinguishable subpopulations of cells.

Abstract: Disclosed is a prostate cancer diagnostic biomarker composition including, as an active ingredient, at least one kynurenine pathway metabolite selected from kynurenine and anthranilate. Also disclosed are a prostate cancer diagnostic composition including an agent capable of measuring the level of the biomarker and a prostate cancer diagnostic kit including the prostate cancer diagnostic composition. The use of the prostate cancer diagnostic biomarker composition can significantly improve the accuracy of prostate cancer diagnosis as an alternative or supplement to the use of PSA levels for prostate cancer diagnosis according to the prior art, which has difficulty in ensuring accurate diagnosis. Therefore, the biomarker composition can be widely used in a variety of industrial applications, including medical applications.

Abstract: A method for selective labelling of necrotic cells including incubating the cells in a solution including a Ln(III)Zn16(HA ligand)16 metallacrown complex, wherein the HA ligand is a hydroximate ligand.

Abstract: Devices, kits, and assays are provided for the testing and monitoring of branched-chain amino acids in biological fluids.

Abstract: Research tool assay for identifying a specific modulator of GNAI, compositions and methods of use. The assay includes combining a drug candidate with GNAI and a C-terminal GIV peptide and determining if the drug candidate inhibits GNAI interaction with the C-terminal GIV peptide; and combining the drug candidate with GNAI and a DAPLE peptide and determining if the drug candidate inhibits GNAI interaction with DAPLE peptide. Also provided are compositions and methods of treatment relating to the same.

Abstract: A method of determining the effect of non-specific interactions in simulated in vivo conditions is presently disclosed. The method includes (a) contacting a solution comprising a biologically relevant molecular crowding agent and a target molecule with a biosensor, wherein the surface of the biosensor comprises a capture molecule that specifically binds the target molecule; (b) allowing the target molecule to bind to the capture molecule; and (c) determining an amount of the target molecule bound to capture molecule using biolayer interferometry.

Abstract: Methods, devices, and systems are disclosed for performing high throughput analysis of conformational change in biological molecules or other biological entities using surface-selective nonlinear optical detection techniques.

Abstract: The present disclosure provides methods and compositions for the determining the abundance and/or concentration of protein biomarkers in a biological sample.