Patents Issued in May 12, 2020
  • Patent number: 10647975
    Abstract: Disclosed herein are modified strains for reducing degradation of recombinantly expressed products secreted from a host organism and methods of using the modified strains. In some embodiments, to attenuate a protease activity in Pichia pastoris, the genes encoding enzymes the degrade proteases are inactivated or mutated to reduce or eliminate activity. In preferred strains, the protease activity of proteases encoded by PAS_chr4_0584 (YPS1-1) and PAS_chr3_1157 (YPS1-2) (e.g., polypeptides comprising SEQ ID NO: 66 and 67) is attenuated.
    Type: Grant
    Filed: October 3, 2017
    Date of Patent: May 12, 2020
    Assignee: Bolt Threads, Inc.
    Inventors: Matthew Scott Gamboa, Joshua Tyler Kittleson
  • Patent number: 10647976
    Abstract: The expression plasmid vectors comprise a polynucleotide sequence encoding Ldc2 polypeptide, a fragment, and/or a mutant. A backbone plasmid is capable of autonomous replication in a host cell. The host cell is not a P. aeruginosa cell. Transformants are transformed with expression plasmid vector. The transformants are not P. aeruginosa. Mutant host cells comprise a polynucleotide sequence encoding Ldc2 polypeptide, a fragment and/or a mutant that has been integrated into the host cell chromosome. A polypeptide, a fragment and/or a mutant comprise Ldc2. A non-naturally occurring polynucleotide, and/or a mutant encodes polypeptide comprising Ldc2. Biobased cadaverine is produced using the transformants and the biobased cadaverine is prepared by the method. Polyamides are formed using the biobased cadaverine and compositions.
    Type: Grant
    Filed: June 26, 2014
    Date of Patent: May 12, 2020
    Assignees: Cathay Biotech Inc., CIBT America Inc
    Inventors: Howard Chou, Naiqiang Li, Xiucai Liu
  • Patent number: 10647977
    Abstract: This invention relates to an engineered leader-independent heterocyclase (also known as a cyclodehydratase) comprising a defined cyanobactin leader sequence which drives the efficient conversion of heterocyclisable amino acids, such as Ser, Thr and Cys, within a peptide substrate lacking a leader sequence into heterocycles produce a homogenous heterocycle-containing product. This may be useful in biotechnology and chemical synthesis.
    Type: Grant
    Filed: November 4, 2015
    Date of Patent: May 12, 2020
    Assignees: THE UNIVERSITY COURT OF THE UNIVERSITY OF ABERDEEN, THE UNIVERSITY COURT OF THE UNIVERSITY OF ST. ANDREWS
    Inventors: James Naismith, Jesko Koehnke, Andrew Bent, Nicholas Westwood, Greg Mann, Wael Houssen Ibrahim, Marcel Jaspars, Ying Ge
  • Patent number: 10647978
    Abstract: A solution for extracting substantially pure RNA from a biological sample is disclosed. The solution for extracting RNA from a biological sample containing RNA and at least DNA comprises: (a) phenol in an amount of more than 50% by volume based on the total amount of the solution; (b) a polyol in an amount of 3 to 10% by volume based on the total amount of the solution; (c) a guanidinium salt at a concentration of 0.5 to 2.0 M based on the total amount of the solution; (d) a thiocyanate at a concentration of 0.1 to 0.5 M based on the total amount of the solution; and (e) a buffer for maintaining the pH of the solution at 4 to 6.
    Type: Grant
    Filed: April 28, 2017
    Date of Patent: May 12, 2020
    Assignee: Toray Industries, Inc.
    Inventors: Makiko Yoshimoto, Hideo Akiyama, Hitoshi Nobumasa
  • Patent number: 10647979
    Abstract: Provided are nucleic acid extraction apparatuses and operation methods thereof. The apparatus may include at least one cyclically moveable annular structure, at least one pipetting mechanism, at least one injection mechanism and a driving mechanism. The annular structure may be provided with a plurality of cuvette positions and a plurality of operation positions. The pipetting mechanism and the injection operation may be arranged along the annular structure. The driving mechanism may drive the annular structure to move cyclically.
    Type: Grant
    Filed: December 9, 2016
    Date of Patent: May 12, 2020
    Assignee: Shenzhen Mindray Bio-Medical Electronics Co., Ltd.
    Inventors: Hai Wang, Yanwen Weng, Yanning Leng, Chuanfen Xie
  • Patent number: 10647980
    Abstract: The present disclosure provides a HTP microbial genomic engineering platform that is computationally driven and integrates molecular biology, automation, and advanced machine learning protocols. This integrative platform utilizes a suite of HTP molecular tool sets to create HTP genetic design libraries, which are derived from, inter alia, scientific insight and iterative pattern recognition. The HTP genomic engineering platform described herein is microbial strain host agnostic and therefore can be implemented across taxa. Furthermore, the disclosed platform can be implemented to modulate or improve any microbial host parameter of interest.
    Type: Grant
    Filed: July 1, 2019
    Date of Patent: May 12, 2020
    Assignee: Zymergen Inc.
    Inventors: Zach Serber, Erik Jedediah Dean, Shawn Manchester, Katherine Gora, Michael Flashman, Erin Shellman, Aaron Kimball, Shawn Szyjka, Barbara Frewen, Thomas Treynor, Kenneth S. Bruno
  • Patent number: 10647981
    Abstract: Methods of forming a library of polynucleotide templates for DNA sequencing are disclosed. The methods include distributing a nucleic acid sample into different droplets in a set of emulsion droplets. The droplets into which the polynucleotide species are distributed include primers which binds to the polynucleotide species and are extended by a polymerase contained within the droplet. A set of extended primers are created simultaneously in a large number of droplets. An enriched set of extended primers are distributed into a second set of emulsion droplets containing a second primer which is extended by a polymerase contained within the second set of droplets.
    Type: Grant
    Filed: September 8, 2016
    Date of Patent: May 12, 2020
    Assignee: Bio-Rad Laboratories, Inc.
    Inventor: John A. Luckey
  • Patent number: 10647982
    Abstract: In an illustrative embodiment, automated multi-module cell editing instruments are provided to automate multiple edits into nucleic acid sequences inside one or more cells.
    Type: Grant
    Filed: January 23, 2020
    Date of Patent: May 12, 2020
    Assignee: Inscripta, Inc.
    Inventors: Don Masquelier, Phillip Belgrader, Jorge Bernate, Ryan Gill, Kevin Ness
  • Patent number: 10647983
    Abstract: The present disclosure relates to compositions and methods for inhibiting nonsense-mediated mRNA decay in a gene-specific manner, for example in the treatment of diseases or disorders caused by nonsense mutations.
    Type: Grant
    Filed: September 4, 2014
    Date of Patent: May 12, 2020
    Assignee: Cold Spring Harbor Laboratory
    Inventors: Adrian Krainer, Isabel Aznarez, Tomoki Nomakuchi
  • Patent number: 10647984
    Abstract: The present invention relates to the use of the miRNA expression profile, particularly of miR-199a-5p, and the target genes regulated thereby for the diagnosis, prognosis and use of miR-199a-5p inhibitors for treating fibroproliferative disorders.
    Type: Grant
    Filed: January 3, 2017
    Date of Patent: May 12, 2020
    Assignees: CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE, CENTRE HOSPITALIER REGIONAL UNIVERSITAIRE DE LILLE
    Inventors: Nicolas Pottier, Bernard Mari, Brice Marcet, Pascal Barbry
  • Patent number: 10647985
    Abstract: The present invention relates to the fields of medicine and immunology. In particular, it relates to novel antisense oligonucleotides that may be used in the treatment, prevention and/or delay of Leber congenital amaurosis.
    Type: Grant
    Filed: November 21, 2018
    Date of Patent: May 12, 2020
    Assignee: STICHTING KATHOLIEKE UNIVERSITEIT
    Inventors: Robert Wilhelmus Johanna Collin, Franciscus Peter Maria Cremers, Antonia Ingrid Den Hollander
  • Patent number: 10647986
    Abstract: The present disclosure relates to an inhibitor of Dynamin 2 for use in the treatment of centronuclear myopathies. The present disclosure relates to pharmaceutical compositions containing Dynamin 2 inhibitor and to their use for the treatment of centronuclear myopathies. It also deals with a method for identifying or screening molecules useful in the treatment of a centronuclear myopathy.
    Type: Grant
    Filed: October 20, 2014
    Date of Patent: May 12, 2020
    Assignees: UNIVERSITE DE STRASBOURG, CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE, INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE
    Inventors: Jocelyn Laporte, Belinda Cowling, Hichem Tasfaout
  • Patent number: 10647987
    Abstract: The present invention relates to a novel approach for treating inflammatory disorders and other diseases, which is based on targeting ROR gamma t mRNA. The invention is directed to oligonucleotides comprising 10 to 22 modified or unmodified nucleotides complementary to specifically selected regions of the RORC, transcript variant 2 mRNA (RORC2), which codes for the ROR gamma t protein.
    Type: Grant
    Filed: February 24, 2017
    Date of Patent: May 12, 2020
    Assignee: Secama Pharmaceuticals GmbH & Co KG
    Inventors: Frank Jaschinski, Ksenija Schirduan, Sven Michel
  • Patent number: 10647988
    Abstract: Described are methods for promoting increase in plant biomass and yield. This increase has its visible effects in organs such as leaf, stem, root and production of fruits and seeds. Further described is the increase in tolerance of those plants to drought, generating plants better adapted to the environmental changes, improving their growth, biomass and yield.
    Type: Grant
    Filed: March 8, 2016
    Date of Patent: May 12, 2020
    Assignee: Universidade Federal Do Rio De Janeiro
    Inventors: Adriana Silva Hemerly, Carinne de Nazaré Monteiro Costa, Luiz Mors Cabral, Vanessa Costa Lurif, Paulo Cavalcanti Gomes Ferreira
  • Patent number: 10647989
    Abstract: The present disclosure provides approaches for reducing tobacco-specific nitrosamines (TSNAs) in tobacco. Some of these approaches include genetically engineering tobacco plants to increase one or more antioxidants, increase oxygen radical absorbance capacity (ORAC), or reduce nitrite. Also provided are methods and compositions for producing modified tobacco plants and tobacco products therefrom comprising reduced TSNAs.
    Type: Grant
    Filed: October 6, 2017
    Date of Patent: May 12, 2020
    Assignee: Altria Client Services LLC
    Inventors: Chengalrayan Kudithipudi, Dongmei Xu, James Strickland
  • Patent number: 10647990
    Abstract: The present invention provides a new plant gene-rice high temperature resistance 1 gene (Rice High Temperature Resistance 1, HTR1) and encoded protein thereof. Also disclosed is the use of the high temperature resistance gene, especially for the enhancement of high-temperature resistance of plants in plant variety improvement and cross breeding.
    Type: Grant
    Filed: May 28, 2015
    Date of Patent: May 12, 2020
    Assignee: SHANGHAI INSTITUTES FOR BIOLOGICAL SCIENCES, CHINESE ACADEMY OF SCIENCES
    Inventors: Hongxuan Lin, Xinmin Li, Jiping Gao, Junxiang Shan, Min Shi
  • Patent number: 10647991
    Abstract: The present invention provides for compositions and methods for producing sorghum crop plants that are resistant to herbicides. In particular, the present invention provides for sorghum plants, plant tissues and plant seeds that contain altered acetolactate synthase (ALS) genes and proteins that are resistant to inhibition by herbicides that normally inhibit the activity of the ALS protein.
    Type: Grant
    Filed: January 26, 2017
    Date of Patent: May 12, 2020
    Assignee: KANSAS STATE UNIVERSITY RESEARCH FOUNDATION
    Inventors: Mitchell R. Tuinstra, Kassim Al-Khatib
  • Patent number: 10647992
    Abstract: The present invention relates to new transporter polypeptides, and genes encoding therefor, which can be used to confer upon a plant resistance to one or more biotrophic fungal pathogens.
    Type: Grant
    Filed: May 3, 2018
    Date of Patent: May 12, 2020
    Assignee: COMMONWEALTH SCIENTIFIC AND INDUSTRIAL RESEARCH ORGANISATION
    Inventors: Evans Lagudah, John Wallace Moore
  • Patent number: 10647993
    Abstract: The present invention belongs to an insecticide compound derived from a strain of Bacillus thuringiensis and refers to the field of controlling plant pests, particularly to the control of the boll weevil—Anthonomus grandis. More specifically, the object of the invention refers to a new gene for the new delta-endotoxin designated Cry8Ha and to the cloning and expression of the gene that encodes for the protein Cry8Ha in Escherichia coli. There is provided the nucleotide sequence and encoding protein of the new delta-endotoxin, recombinant vectors and host cells. There is also provided processes and means for recombinant production and the use of the new delta-endotoxin for application in the control of the boll weevil. Additionally, the invention also provides an optimized synthetic gene for expression in cotton plants. Using the gene described herein, it is possible to transform plants based on techniques known by specialists in the art, for the expression of the endotoxin active against the boll weevil.
    Type: Grant
    Filed: July 26, 2010
    Date of Patent: May 12, 2020
    Assignee: EMPRESA BRASILEIRA DE PESQUISA AGROPECUARIA—EMBRAPA
    Inventors: Maria Fatima Grossi De Sa, Gustavo Ramos De Oliveira, Maria Cristina Mattar Da Silva, Thales Lima Rocha, Mariana Torquato Quezado De Magalhaes
  • Patent number: 10647994
    Abstract: This disclosure concerns nucleic acid molecules and methods of use thereof for control of coleopteran and/or hemipteran pests through RNA interference-mediated inhibition of target coding and transcribed non-coding sequences in coleopteran and/or hemipteran pests. The disclosure also concerns methods for making that express nucleic acid molecules useful for the control of coleopteran and/or hemipteran pests, and the plant cells and plants obtained thereby.
    Type: Grant
    Filed: December 19, 2014
    Date of Patent: May 12, 2020
    Assignee: Dow AgroSciences LLC
    Inventors: Kenneth E. Narva, Huarong Li, Chaoxian Geng, Kanika Arora, Balaji Veeramani, Premchand Gandra, Sarah Worden, Andreas Vilcinskas, Eileen Knorr, Elane Fishilevich, Murugesan Rangasamy, Meghan Frey
  • Patent number: 10647995
    Abstract: The present invention relates generally to methods of molecular biology and gene silencing to control pests.
    Type: Grant
    Filed: June 16, 2016
    Date of Patent: May 12, 2020
    Assignees: PIONEER HI-BRED INTERNATIONAL, INC., E I DU PONT DE NEMOURS AND COMPANY
    Inventors: Xu Hu, Bliss Marie Kernodle, Molly Mary McMahon, Adane Kassa, Xiping Niu, James Kevin Presnail, Albert L. Lu, Nina Richtman, Jonathan William Robeson
  • Patent number: 10647996
    Abstract: Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. The toxin coding sequences can be used in DNA constructs or expression cassettes for expression in plants and bacteria. Compositions include transformed bacteria, plants, plant cells, tissues, and seeds. In particular, polynucleotide sequences and the toxin proteins encoded thereby are provided. Also provided are antibodies specifically binding to those amino acid sequences. In particular, the invention encompasses nucleotide sequences encoding fusion proteins, as well as biologically active variants and fragments thereof, wherein the fusion protein contains the C-terminal portion of SEQ ID NO:43. The fusion protein may also contain the N-terminal portion of SEQ ID NO:45.
    Type: Grant
    Filed: April 24, 2018
    Date of Patent: May 12, 2020
    Assignee: BASF Agricultural Solutions Seed US LLC
    Inventors: Nalini Manoj Desai, Volker Heinrichs, Duane Lehtinen
  • Patent number: 10647997
    Abstract: A modified effector cell includes a non-reversibly produced vector-encoded anti-GD2-BB-? chimeric receptor for use in disialoganglioside GD2-expressing neoplasia, which is inserted in the cell, to obtain an effector cell that stably produces the anti-GD2-BB-? chimeric receptor, the chimeric receptor having two distinct mutually fused portions, i.e. an intra-cytoplasmic portion and an extra-cytoplasmic portion.
    Type: Grant
    Filed: October 25, 2012
    Date of Patent: May 12, 2020
    Assignee: St. Jude Children's Research Hospital, Inc.
    Inventors: Massimo Dominici, Sara Caldrer, Maria Carlotta Spano, Paolo Paolucci, Marco Bestagno, Dario Campana
  • Patent number: 10647998
    Abstract: Described herein are synonymously altered gene sequences which express protein in differing levels within secretory as compared to non-secretory target tissue. An expression cassette comprising an open reading frame (ORF) for a protein under the control of regulatory sequences which direct expression of the product in cell, which ORF has been modified to preferentially increase expression levels in a selected tissue, wherein the modified ORF is characterized by a triplet frequency of any one of Tables 3-12, 16 or 17.
    Type: Grant
    Filed: June 20, 2017
    Date of Patent: May 12, 2020
    Assignee: The Trustees of the University of Pennsylvania
    Inventors: James M. Wilson, Anna Tretiakova
  • Patent number: 10647999
    Abstract: The present invention relates to nucleic acid molecules which are capable of promoting transcription of operably-linked heterologous polynucleotides in mammalian cells. The invention also relates to expression vectors and host cells which comprise the nucleic acid molecules of the invention. Such expression vectors may be used to produce recombinant proteins, e.g. antibodies and lentiviral polypeptides.
    Type: Grant
    Filed: February 28, 2017
    Date of Patent: May 12, 2020
    Assignee: Oxford Genetics Limited
    Inventors: Ryan Cawood, Richard Parker-Manuel, Weiheng Su
  • Patent number: 10648000
    Abstract: Disclosed are capsid-modified rAAV expression vectors, as well as infectious virions, compositions, and pharmaceutical formulations that include them. Also disclosed are methods of preparing and using novel capsid-protein-mutated rAAV vector constructs in a variety of diagnostic and therapeutic applications including, inter alia, as delivery agents for diagnosis, treatment, or amelioration of one or more diseases, disorders, or dysfunctions of the mammalian vascular system, and complications from Type I diabetes. Also disclosed are methods for systemic and tissue-localized delivery of therapeutic rAAV-based gene expression cassettes to vascular endothelial cells, tissues, and organs, as well as use of the disclosed compositions in the manufacture of medicaments for a variety of in vitro and/or in vivo applications including the treatment of vasculitis, and complications arising from Type I diabetes, such as macular edema, nephropathy, diabetic retinopathy, and the like.
    Type: Grant
    Filed: February 16, 2016
    Date of Patent: May 12, 2020
    Assignee: University of Florida Research Foundation, Incorporated
    Inventors: William W. Hauswirth, Sanford L. Boye, Daniel M. Lipinski, Michael E. Boulton, Shannon E. Boye
  • Patent number: 10648001
    Abstract: Nucleases and methods of using these nucleases for inserting a sequence encoding a therapeutic protein such as an enzyme into a cell, thereby providing proteins or cell therapeutics for treatment and/or prevention of a lysosomal storage disease.
    Type: Grant
    Filed: October 1, 2015
    Date of Patent: May 12, 2020
    Assignee: Sangamo Therapeutics, Inc.
    Inventor: Edward J. Rebar
  • Patent number: 10648002
    Abstract: Methods of gene correction, methods of generating induced pluripotent stem cells (iPSCs), and methods of deriving multi-lineage cell types with therapeutic value. In some embodiments, the gene correction affects the expression and/or function of the functional type VII collagen protein (C7).
    Type: Grant
    Filed: November 22, 2017
    Date of Patent: May 12, 2020
    Assignee: REGENTS OF THE UNIVERSITY OF MINNESOTA
    Inventors: Beau R. Webber, Mark J. Osborn, Jakub Tolar, Bruce R. Blazar
  • Patent number: 10648003
    Abstract: The present invention provides a method for obtaining site-specific recombination in a eukaryotic cell, the method comprising providing a eukaryotic cell that comprises a first recombination attachment site and a second recombination attachment site; contacting the first and second recombination attachment sites with a prokaryotic recombinase polypeptide, resulting in recombination between the recombination attachment sites, wherein the recombinase polypeptide can mediate recombination between the first and second recombination attachment sites, the first recombination attachment site is a phage genomic recombination attachment site (attP) or a bacterial genomic recombination attachment site (attB), the second recombination site is attB or attP, and the recombinase is selected from the group consisting of a Listeria monocytogenes phage recombinase, a Streptococcus pyogenes phage recombinase, a Bacillus subtilis phage recombinase, a Mycobacterium tuberculosis phage recombinase and a Mycobacterium smegmatis pha
    Type: Grant
    Filed: June 8, 2018
    Date of Patent: May 12, 2020
    Assignee: Intrexon Corporation
    Inventor: Malla Padidam
  • Patent number: 10648004
    Abstract: The invention features compositions and methods for the increased production of mevalonate, isoprene, isoprenoid precursor molecules, isoprenoids, and/or acetyl-CoA-derived products in recombinant microorganisms by engineering the microorganisms to comprise one or more acetylating proteins such that the expression and/or activity of the one or more acetylating proteins is modulated.
    Type: Grant
    Filed: February 20, 2015
    Date of Patent: May 12, 2020
    Assignees: Danisco US Inc., The Goodyear Tire & Rubber Company
    Inventors: Marguerite A. Cervin, Michael C. Miller, Derek H. Wells, Alan J. Wolfe, Madhukar S. Dasika
  • Patent number: 10648005
    Abstract: The present disclosure relates to the transformed Synechococcus elongatus strain of capable of mass production of farnesene. The transformed Synechococcus elongatus strain of the present disclosure is characterized by having the ability to mass produce farnesene using carbon dioxide as an independent carbon source. In particular, the Synechococcus elongatus strain is economically effective because it uses carbon dioxide present in light and air as a carbon source. There is an eco-friendly effect since it can be used for eliminating or reducing carbon dioxide in the atmosphere using microorganisms. Further, the strain of the present disclosure has a rapid growth rate and excellent ability to fix carbon dioxide compared with other microorganisms, thereby being utilized in various fields such as food, medicine, pharmacy, biofuel, and chemistry.
    Type: Grant
    Filed: October 2, 2018
    Date of Patent: May 12, 2020
    Assignee: Research & Business Foundation Sungkyunkwan University
    Inventors: Han-Min Woo, Hyun-Jeong Lee
  • Patent number: 10648006
    Abstract: This invention relates to modified hydroxylases. The invention further relates to cells expressing such modified hydroxylases and methods of producing hydroxylated alkanes by contacting a suitable substrate with such cells.
    Type: Grant
    Filed: March 30, 2018
    Date of Patent: May 12, 2020
    Assignee: California Institute of Technology
    Inventors: Frances Arnold, Peter Meinhold, Matthew W. Peters, Rudi Fasan, Mike M. Y. Chen
  • Patent number: 10648007
    Abstract: Provided is an enzymatic process for the preparation of a mercaptan of formula R—SH from disulfides utilizing hydrogen.
    Type: Grant
    Filed: September 29, 2016
    Date of Patent: May 12, 2020
    Assignee: ARKEMA FRANCE
    Inventors: Georges Fremy, Arnaud Masselin, Hugo Brasselet
  • Patent number: 10648008
    Abstract: The present invention relates to a process for the production of second generation biofuels and/or sugar based chemicals—for example ethanol, butanol etc—and/or materials—for example plastics, single cell proteins etc.—together with sulfonated lignin from lignocellulosic biomass, in particular from lignocellulosic biomass comprising, among others, annual plants, agricultural waste, or wood. In particular, the present invention relates to a process for the production of sugar based chemicals, biofuels or materials together with sulfonated lignin from lignocellulosic biomass comprising the pretreatment of a lignocellulosic biomass in a sulfite cooking step.
    Type: Grant
    Filed: December 16, 2009
    Date of Patent: May 12, 2020
    Assignee: Borregaard Industries, Limited, Norge
    Inventors: Anders Sjoede, Anders Froelander, Martin Lersch, Gudbrand Roedsrud
  • Patent number: 10648009
    Abstract: The present invention relates to enzyme compositions for high temperature saccharification of cellulosic material and to uses thereof.
    Type: Grant
    Filed: August 29, 2018
    Date of Patent: May 12, 2020
    Assignee: Novoyzmes, Inc.
    Inventors: Brett McBrayer, Tarana Shaghasi, Elena Vlasenko
  • Patent number: 10648010
    Abstract: This invention provides for an improved method of stabilizing freeze dried bacterial extracts with a carbohydrate lyoprotectant such that the extracts can be for use in cell free protein synthesis. Also provided herein are formulations for stable, freeze dried bacterial extracts that when stored at room temperature retain at least about 70% protein synthesis activity compared to undried frozen bacterial extracts.
    Type: Grant
    Filed: January 8, 2016
    Date of Patent: May 12, 2020
    Assignee: SUTRO BIOPHARMA, INC.
    Inventors: Martin Chandler, Kirk Hayenga, Henry Heinsohn
  • Patent number: 10648011
    Abstract: The present invention provides members that produce on a large scale a coenzyme-linked glucose dehydrogenase which has excellent substrate-recognizing ability toward glucose while providing low action on maltose. The present invention relates to a polynucleotide encoding a soluble coenzyme-linked glucose dehydrogenase that catalyzes the oxidation of glucose in the presence of an electron acceptor and has an activity toward maltose of 5% or lower; a polypeptide encoded by the nucleotide sequence of the polynucleotide; a recombinant vector carrying the polynucleotide; a transformed cell produced using the recombinant vector; a method for producing a polypeptide comprising culturing the transformed cell and collecting from the cultivated products a polypeptide that links to FAD to exert the glucose dehydration activity; a method for determination of glucose using the polypeptide; a reagent composition for determination of glucose; and a biosensor.
    Type: Grant
    Filed: March 21, 2018
    Date of Patent: May 12, 2020
    Assignees: Ikeda Food Research Co., ltd., PHC Corporation
    Inventors: Hironori Omura, Hirokazu Sanada, Takako Yada, Ayaka Atsumi, Tetsunari Morita, Emi Ishimaru
  • Patent number: 10648012
    Abstract: Methods and systems for identifying inhibitors of Equilibrative Nucleoside Transporters are provided. Methods and systems for identifying inhibitors of Concentrative Nucleoside Transporters are also provided.
    Type: Grant
    Filed: January 8, 2014
    Date of Patent: May 12, 2020
    Assignee: Albert Einstein College of Medicine
    Inventors: Myles Akabas, Ithiel James Frame, Roman Deniskin
  • Patent number: 10648013
    Abstract: Provided is a compound that comprises the structure: where SIG is a signaling molecule and R3 is a formyl, a succinyl, a methyl succinyl, or a myristoyl. Also provided is a kit is provided that comprises the above compound, with instructions for determining the presence of the enzyme. Additionally, a method is provided for determining whether a sample has an enzyme that removes a succinyl, a methyl succinyl, a formyl, or a myristoyl moiety from an ?-amino of a lysine. Also provided is a method of determining whether a molecule inhibits an enzyme that removes a succinyl, a methyl succinyl, a formyl, or a myristoyl moiety from an ?-amino of a lysine.
    Type: Grant
    Filed: April 18, 2019
    Date of Patent: May 12, 2020
    Assignee: Enzo Life Sciences, Inc.
    Inventors: Konrad T. Howitz, Zhongda Zhang, Anne Kisielewski, Elizabeth Dale, Wayne Forrest Patton
  • Patent number: 10648014
    Abstract: Disclosed are compositions and methods for measuring tyrosine kinase activity using degradation resistant biosensors comprising a reporter sequence, a target sequence, and a transduction sequence. In certain embodiments, the invention includes a biosensor comprising a peptide comprising a reporter sequence, i.e., an amino acid sequence including a tyrosine residue that can be phosphorylated by a tyrosine kinase. In certain embodiments, a biosensor comprises a reporter sequence configured to resist degradation from proteases, a targeting sequence coupled to the reporter sequence and configured to bind to a protein interaction domain of a kinase, and a transduction sequence coupled to the targeting sequence.
    Type: Grant
    Filed: April 29, 2015
    Date of Patent: May 12, 2020
    Assignee: Purdue Research Foundation
    Inventors: Laurie L Parker, Robert John Schuster
  • Patent number: 10648015
    Abstract: A blood glucose measurement reagent includes: an enzyme having glucose as a substrate; a mediator; a chromogenic indicator; and an aromatic hydrocarbon having at least one sulfonic acid group. A sensor chip includes: a supply port through which blood is suppliable; a flow path having the supply port formed at one end thereof; and a reagent located in the flow path, the reagent being the blood glucose measurement reagent; wherein the sensor chip is mountable on a blood glucose meter for measuring blood glucose in the blood.
    Type: Grant
    Filed: June 29, 2018
    Date of Patent: May 12, 2020
    Assignee: TERUMO KABUSHIKI KAISHA
    Inventors: Hiroya Sato, Takeyuki Moriuchi, Yusuke Komata
  • Patent number: 10648016
    Abstract: A method of detecting a state of a lipid membrane in a cell of a nanopore based sequencing chip is disclosed. A lipid membrane is coupled with an integrating capacitor, wherein the lipid membrane is between a working electrode and a counter electrode. An alternating current (AC) voltage is applied to the counter electrode. A voltage across the integrating capacitor is periodically sampled by an analog-to-digital converter (ADC). A change in the sampled voltage across the integrating capacitor in response to an intermediate change in the AC voltage is determined. A state of the lipid membrane is determined based on the determined change in the sampled voltage across the integrating capacitor in response to the intermediate change in the AC voltage.
    Type: Grant
    Filed: January 29, 2019
    Date of Patent: May 12, 2020
    Assignee: Roche Sequencing Solutions, Inc.
    Inventor: Ashraf Wahba
  • Patent number: 10648017
    Abstract: The present invention relates to the field of RNA analysis. In particular, the invention concerns the use of a catalytic nucleic acid molecule for the analysis of an RNA molecule. The invention concerns methods for analyzing the 5? terminal structures of an RNA molecule having a cleavage site for a catalytic nucleic acid molecule.
    Type: Grant
    Filed: June 28, 2016
    Date of Patent: May 12, 2020
    Assignee: CureVac Real Estate GmbH
    Inventor: Aniela Wochner
  • Patent number: 10648018
    Abstract: Aspects of the present disclosure include systems that include reaction vessels and reaction vessel caps. In certain aspects, the reaction vessels include a reaction chamber and a groove disposed around a top opening of a reaction chamber. The system also includes a RV cap that includes a cap body, a RV plug, and a lower wall that includes an outer radial groove disposed above an outward projecting ridge of the lower wall. When the cap is inserted into the RV, the RV plug of the RV cap is sealingly inserted into the reaction chamber of the RV, a ridge of the RV mates with the outer radial groove of the RV cap, and an outward projecting ridge of the RV cap mates with the radial groove of the RV. Also provided are methods and sample analysis systems, which may employ the RV/RV cap systems of the present disclosure.
    Type: Grant
    Filed: March 15, 2017
    Date of Patent: May 12, 2020
    Assignee: Abbott Molecular Inc
    Inventors: Sonal Sadaria Nana, Paul M. Grippo, Eric D. Yeaton, Jason E. Bryant, Mark Talmer, Kavankumar B. Patel, Wesley W. Addison, II
  • Patent number: 10648019
    Abstract: Disclosed is a probe mixture for real-time detection of target nucleic acids comprising at least one detection probe and at least one clamping probe for inhibiting amplification of wild type genes or unwanted genes, a kit using the same and a method for real-time detection of target nucleic acids using the mixture and the kit.
    Type: Grant
    Filed: October 14, 2014
    Date of Patent: May 12, 2020
    Assignee: PANAGENE INC.
    Inventors: Goon Ho Joe, Yong Tae Kim, Sung Kee Kim, Jin Woo Kim, Jae Jin Choi, In Seong Wo, Ji Hye Yoon, Su Nam Kim, Jun Ho Park
  • Patent number: 10648020
    Abstract: The invention provides for systems, methods, and compositions for targeting nucleic acids. In particular, the invention provides non-naturally occurring or engineered DNA-targeting systems comprising a novel DNA-targeting CRISPR effector protein and at least one targeting nucleic acid component like a guide RNA. Methods for making and using and uses of such systems, methods, and compositions and products from such methods and uses are also disclosed and claimed.
    Type: Grant
    Filed: December 17, 2017
    Date of Patent: May 12, 2020
    Assignees: THE BROAD INSTITUTE, INC., MASSACHUSETTS INSTITUTE OF TECHNOLOGY, PRESIDENT AND FELLOWS OF HARVARD COLLEGE
    Inventors: Feng Zhang, Bernd Zetsche, Jonathan S. Gootenberg, Omar O Abudayyeh, Ian Slaymaker
  • Patent number: 10648021
    Abstract: Disclosed are methods and compositions for detection and amplification of nucleic acids, wherein two DNA strands hybridized to an RNA strand are ligated. In one aspect, the disclosed methods include removal of an energy source, such as ATP, upon charging a ligase to form an enzyme-AMP intermediate, and then adding substrate, which results in one complete round of RNA-templated DNA ligation. In another aspect, the ligation reaction is accomplished by use of a mixture of at least two different ligase enzymes. The disclosed methods and compositions for RNA-templated DNA ligation may be particularly useful for detection of RNA sequence variants, for example RNA splice variants, and for quantitative expression analysis.
    Type: Grant
    Filed: October 30, 2017
    Date of Patent: May 12, 2020
    Assignee: AFFYMETRIX, INC.
    Inventors: Eugeni A. Namsaraev, Xin Miao, John E. Blume
  • Patent number: 10648022
    Abstract: A composition includes a nanopore including first and second sides and an aperture, nucleotides each including an elongated tag, and a first polynucleotide that is complementary to a second polynucleotide. A polymerase can be disposed adjacent to the first side of the nanopore and configured to add nucleotides to the first polynucleotide based on a sequence of the second polynucleotide. A permanent tether can include a head region anchored to the polymerase, a tail region, and an elongated body disposed therebetween that occurs in the aperture of the nanopore. A first moiety can be disposed on the elongated body that binds to the elongated tag of a first nucleotide upon which the polymerase is acting. A reporter region can be disposed on the elongated body that indicates when the first nucleotide is complementary or is not complementary to a next nucleotide in the sequence of the second polynucleotide.
    Type: Grant
    Filed: June 2, 2016
    Date of Patent: May 12, 2020
    Assignee: Illumina, Inc.
    Inventors: Kevin L. Gunderson, Jeffrey G. Mandell
  • Patent number: 10648023
    Abstract: The invention provides systems and methods for processing samples. In a method, a reaction card is provided that has a channel network, a valve, and a micropump, all disposed within the card. The reaction card also has a collection well disposed on a surface of the card and a tubular member extending out from the card. A reaction vessel is provided and affixed to the reaction card such that the tubular member is inserted into the reaction vessel. Amplification reaction reagents and a sample are delivered into the reaction vessel, and an amplification reaction is initiated within the reaction vessel, resulting in an amplification product being disposed within the reaction vessel. The valve is opened to atmosphere, and the first micropump is activated to pump an aliquot of reaction product from the reaction vessel into the tubular member, through the channel network, and into the collection well.
    Type: Grant
    Filed: June 27, 2017
    Date of Patent: May 12, 2020
    Assignee: CALIPER LIFE SCIENCES, INC.
    Inventors: Edward H. Thorne, IV, Abbie L. Esterman
  • Patent number: 10648024
    Abstract: A porous structure according to the present invention has a polymerase chain reaction (PCT) primer inside pores thereof, and hence, even an inner portion thereof can be used unlike general structures of which only surfaces are used for amplification and detection, thereby maximizing reactivity. In addition, the differentiating of the kinds of primers contained in respective structures leads to detection of several kinds of target nucleic acids at the same and real-time analysis thereof at the same time, and thus is useful for multiplex real-time PCR.
    Type: Grant
    Filed: October 28, 2014
    Date of Patent: May 12, 2020
    Assignee: Korea Institute of Science and Technology
    Inventors: Sang Kyung Kim, Nakwon Choi, Dong Jin Lee, Seungwon Jung