Abstract: This disclosure relates to containers (e.g., in the form of bags) comprising a fluoropolymer and containing a degradable carrier comprising biological agent-capturing moieties, to cell isolation systems comprising such containers, and to methods for cultivating a biological agent using such containers. In one embodiment, the disclosure provides a container having an outer surface and an inner surface, the inner surface comprising a fluoropolymer, and contained in the container, a degradable carrier; wherein the carrier comprises a plurality of biological agent-capturing moieties.

Abstract: The invention relates to methods for conducting binding assays in an assay device that includes one or more storage and use zone. The storage zones of the assay device are configured to house one or more reagents used in an assay conducted in the use zone of the device.

Abstract: Provided herein are devices, systems, and methods for purification of sample components. In particular, provided herein are lateral flow devices, systems, and methods that utilize flow control of sample handling.

Abstract: The disclosed embodiments concern methods, devices, and systems for identifying candidate biomarkers useful for the diagnosis, prognosis, monitoring and screening and/or as targets for the treatment of diseases and conditions in subjects, in particular autoimmune and infectious diseases. The identification of candidate biomarkers is predicated on identifying discriminating peptides present on a peptide array, which can distinguish samples from different subjects having different health conditions by the binding patterns of antibodies present in the samples.

Abstract: Disclosed herein are methods of treatment of autoimmune diseases such as systemic lupus erythematosus (SLE) as well as clinical assays for detection of autoimmune disease activity in patients utilizing a PD1 ligand.

Abstract: Disclosed herein are methods of detecting microbial infection in mammalian subjects comprising treatment of a sample and detection of polysaccharide antigenic components. The methods disclosed provide for pretreatment of biological samples, such as urine samples, to maximize detection of galF—containing antigens and improvement of sensitivity of galF antigen detection assays. The methods include minimizing Intelectin-1 binding to galF—containing antigens and improvement of monoclonal antibody binding. The detection methods are useful for identifying the presence of microbial antigens related to Streptococcus pneumoniae, Klebsiella pneumonia, Escherichia coli, Mycobacteria species, Malassezia species, Aspergillus species, Fusarium species, Alternaria species, Coccidioides species, Cryptococcus species, Mucormycetes, Histoplasma species, Neosartorya species, Fusarium species, Paracoccidioides species, or combinations thereof.

Abstract: Light-generating fusion proteins having a ligand binding site and a light-generating polypeptide moiety and their use as diagnostics, in drug screening and discovery, and as therapeutics, are disclosed. The light-generating fusion protein has a feature where the bioluminescence of the polypeptide moiety changes upon binding of a ligand at the ligand binding site. The ligand may be, for example, an enzyme present in an environment only under certain conditions, e.g., ubiquitin ligase in a hypoxic state, such that the light-generating fusion protein is “turned on” only under such conditions.

Abstract: The invention generally relates to immunotherapy with chimeric antigen receptor (CAR}- engineered T-cells. In particular, the invention relates immunotherapy with chimeric antigen receptor (CAR)-engineered T-cells to target sub-populations of cancer cells that are characterized by low expression of a cancer cell surface antigen, more particular the invention relates to immunotherapy with chimeric antigen receptor (CAR)-engineered T-cells targeting CD19 (CD19CART) in multiple myeloma, a clonal proliferation of plasma cells.

Abstract: Provided herein are methods for classifying how a subject having a cancer will respond to immunotherapeutic (IT) therapy based on the subject's immunosignature or frameshift signature. Also provided herein are methods for classifying a subject having a cancer as having a good prognosis or a poor prognosis based on the subject's immunosignature or frameshift signature.

Abstract: The present invention provides methods and compositions for detection, diagnosis, prognosis, monitoring treatment and monitoring progression of cancer by detecting the level of Ki-67 protein in an individual.

Abstract: The present invention, in some embodiments thereof, generally relates to methods and devices for determining the health status of a subject, e.g., whether the subject has a disease or other condition. In some embodiments, a plurality or mixture of species may be differentially solubilized in a single two-phase aqueous system, or other multi-phase aqueous system. The nature or degree of the solubilization of the species may be used to determine the health status of a subject. For example, some embodiments are directed to devices and methods for determining a disease or other condition as a function of the changes to the structure of two or more species. The species may be selected based on their differential solubility behavior in a two-phase or other multi-phase aqueous system.

Abstract: The present invention provides a tumor blood marker and a use thereof. Specifically, the present invention provides a use of a reagent, which is used to detect GAPDH in a blood sample, in a preparation of a detecting composition for tumor screening, risk evaluation of tumor development in subjects, distinction of tumor progression stages, identification of therapeutic efficacy of tumor and/or risk analysis of tumor progression. The present invention also provides a kit and a method for detecting GAPDH concentrations in blood samples.

Abstract: This document relates to materials and methods for assessing and/or treating subjects (e.g., subjects having autoimmune diseases). For example, materials and methods for determining if a subject (e.g., a human having an autoimmune disease) has one or more antibodies that can be used to identify the subject as having a lower risk of cancer or as having a higher risk of cancer are provided. Materials and methods for treating a subject (e.g., a human) identified as having a higher cancer risk for cancer are also provided.

Abstract: The present invention relates to the field of production, identification and selection of biological binding molecules such as antibodies or fragments thereof, as well as to diagnostic procedures using them in the diagnosis of cancers, in particular malignant B-cell neoplasia. The binding molecules are able to selectively bind to autonomously active or autonomously activated B-cell receptors, the autonomously active or autonomously activated B-cell receptors being characterized by the presence of structural domains to which the binding molecule selectively binds and which are responsible for the autonomously active or autonomously activated state of the B-cell receptors.

Abstract: In certain embodiments, the present invention provides a method of treating cancer in a patient comprising administering an effective amount of a therapeutic agent to the patient, wherein the cancer was determined to contain cells comprising cell marker CD24+. In certain embodiments, the cancer cells also comprise cell markers CD38+ and/or CD45?.

Abstract: Methods for diagnosing cancer in a subject are disclosed. The method includes detecting the level of expression of one or more cancer markers in a biological sample obtained from the subject; and comparing the level of expression of the one or more cancer markers in the biological sample to a normal level of expression of the one or more cancer markers. The one or more cancer markers comprises CXCL13 or CXCR5 or both CXCL13 and CXCR5. Also disclosed is a kit for detecting cancer or monitoring cancer progression.

Abstract: Aspects of the application provide methods of identifying and sequencing proteins, polypeptides, and amino acids, and compositions useful for the same. In some aspects, the application provides methods of obtaining data during a degradation process of a polypeptide, and outputting a sequence representative of the polypeptide. In some aspects, the application provides amino acid recognition molecules comprising a shielding element that enhances photostability in polypeptide sequencing reactions.

Abstract: The disclosure provides methods of analyzing an analyte of interest in a biological sample using fluorescent agents and macroconjugates which comprise a core containing a cross-linked polymer or protein, tags, specific binding members or fragments thereof, and optionally carrier proteins. Also provided are methods of analyzing two or more analytes of interest in a biological sample in a single assay using microparticles and detection conjugates comprising different fluorophore labels, acquiring transmitted light and fluorescent images of the microparticles, and using a customized image analysis process to analyze the acquired images.

Abstract: The present invention relates to methods for predicting whether peptides or polypeptides comprising disease specific amino acid modifications, in particular tumor-associated neo-antigens, comprise epitopes, in particular tumor-associated neo-epitopes, which are useful for immunotherapy such as for vaccination. The methods of the invention may be used, in particular, for the provision of vaccines which are specific for a patient's tumor and, thus, in the context of personalized cancer vaccines.

Abstract: A method comprising the steps of: (a) collecting a stool sample with the analyte and transferring a defined amount of stool sample into a prepared vessel having a sieve filter and a predetermined amount of extraction solution; (b) suspending and extracting the stool sample in the extraction solution so that the analyte goes into solution; (c) filtering the extraction solution through the sieve filter and transferring a defined amount of extraction solution to a cellulosic fibrous web having predetermined absorbency; (d) rapid drying of the extraction solution on the cellulose fibrous web at ambient temperature by the capillary action of the fibrous web, wherein the fibrous web with the sample extraction solution represents a storage and transport form stable over days and weeks, on which analyte and digestive enzymes are physically separated from each other; (e) collecting and extracting the analyte from the fibrous web in a predetermined amount of assay buffer; (f) separating the fibrous web from the assay b

Abstract: Aspects of the application provide methods of identifying and sequencing proteins, polypeptides, and amino acids, and compositions useful for the same. In some aspects, the application provides methods of obtaining data during a degradation process of a polypeptide, and outputting a sequence representative of the polypeptide. In some aspects, the application provides amino acid recognition molecules comprising a shielding element that enhances photostability in polypeptide sequencing reactions.

Abstract: Aspects of the application provide methods of identifying and sequencing proteins, polypeptides, and amino acids, and compositions useful for the same. In some aspects, the application provides methods of obtaining data during a degradation process of a polypeptide, and outputting a sequence representative of the polypeptide. In some aspects, the application provides amino acid recognition molecules comprising a shielding element that enhances photostability in polypeptide sequencing reactions.

Abstract: Aspects of the application provide methods of identifying and sequencing proteins, polypeptides, and amino acids, and compositions useful for the same. In some aspects, the application provides methods of obtaining data during a degradation process of a polypeptide, and outputting a sequence representative of the polypeptide. In some aspects, the application provides amino acid recognition molecules comprising a shielding element that enhances photostability in polypeptide sequencing reactions.

Abstract: Aspects of the application provide methods of identifying and sequencing proteins, polypeptides, and amino acids, and compositions useful for the same. In some aspects, the application provides methods of obtaining data during a degradation process of a polypeptide, and outputting a sequence representative of the polypeptide. In some aspects, the application provides amino acid recognition molecules comprising a shielding element that enhances photostability in polypeptide sequencing reactions.

Abstract: Aspects of the application provide methods of identifying and sequencing proteins, polypeptides, and amino acids, and compositions useful for the same. In some aspects, the application provides methods of obtaining data during a degradation process of a polypeptide, and outputting a sequence representative of the polypeptide. In some aspects, the application provides amino acid recognition molecules comprising a shielding element that enhances photostability in polypeptide sequencing reactions.

Abstract: The invention relates to a method for binding or capturing autoantibodies directed to various Glycoprotein 2 (GP2) isoforms. In particular the invention provides an in vitro method for the diagnosis of an autoimmune disorder by the detection of autoantibodies that bind one or more isoforms of GP2. The invention is characterized by the provision of multiple isoforms of GP2 as autoantibody targets and encompasses the practical utilization of the finding that the isoform specificity of anti-GP2 autoantibodies enables determination of particular autoimmune diseases. The invention also provides a system and kit developed for carrying out the claimed method. The present invention is useful for determining whether a sample from an individual comprises autoantibodies associated with an autoimmune disease, and for differentiating between multiple autoimmune diseases that exhibit similar symptoms, such as Celiac disease (CeD), Crohn's disease (CD), primary sclerosing cholangitis (PSC), and/or ulcerative colitis (UC).

Abstract: Methods for identifying compounds that modulate polycystin-1/polycystin-2 (PKD1/PKD2) ion channel activity or cilium/plasma membrane trafficking are provided. Related reagents and uses of the compounds are also provided.

Abstract: The invention relates to a process for the in vitro diagnosis or prognosis of pre-eclampsia, comprising determining an increase or decrease in the concentration of one or more markers in a sample of biological fluid.

Abstract: Embodiments of the disclosure include systems, methods, and compositions for detection of imminent onset of a symptom of a gut inflammation medical condition. The disclosure also concerns microbial biosensors that detect a marker in the gut that is predictive of onset of at least one symptom of inflammatory bowel disease (IBD), for example, and such a sensor may include a promoter sensitive to the marker that is linked to expression of a detectable readout, such as in the feces of the individual with IBD.

Abstract: A method for determining healing status of a wound is provided comprising: i) quantifying the expression level of GM-CSF or MMP-13, and optionally one or more additional biomarkers, in a wound tissue sample or wound fluid sample from a wound of a mammalian subject; and ii) comparing the expression level of GM-CSF or MMP-13 of the wound tissue sample or wound fluid sample to a threshold level and determining that the wound is non-healing if the level of GM-CSF or MMP-13 exceeds the threshold level.

Abstract: The present invention provides compositions and methods directed to incompatible blood group antigens. In particular, the present invention relates to anti-incompatible BG-A antibody molecules targeting a novel immune epitope in the incompatible BG-A antigen, such as the epitope bound by CRC-A1. The invention also relates to nucleic acids encoding such antibody molecules; to host cells expressing or capable of expressing such antibody molecules; to compositions comprising such antibody molecules or fragments thereof; and to uses of such antibody molecules or such compositions, in particular for therapeutic and detection purposes in the field of cancer diseases.

Abstract: Provided herein are compositions, systems, kits, and methods for detecting cardiovascular disease, risk of cardiovascular disease, and/or reverse cholesterol transport potential in a subject based on the levels of PIP2 phospholipid in the subject.

Abstract: The surface of an analysis port of an automatic analyzer, in which a reaction vessel is placed, is configured so as to reflect at least some of the light emitted from a light source. In a first state before the transfer of the reaction vessel to the analysis port by a reaction vessel transfer mechanism, the control unit causes the light source to irradiate light and causes a detector to detect light. In a second state after the transfer of the reaction vessel to the analysis port by the reaction vessel transfer mechanism, the control unit causes the light source to irradiate light and causes the detector to detect light. If the difference between the amount of light detected in the first state and the amount of light detected in the second state is less than or equal to a standard value, the control unit produces a warning.

Abstract: A system for detecting mold includes a housing defining a chamber and an opening through a surface. The system includes a movable grate for selectively covering the opening. The system includes a substrate treated to promote mold growth. The system includes a mechanism for moving the substrate to move previously unexposed portions into the chamber. The system includes a thermal control system to maintain predetermined environmental conditions in the chamber. The system includes a sensor configured to detect mold growth in the chamber. The system includes a mold suppressor to kill mold in the chamber when activated. The system includes a controller to coordinate operation of the components to detect mold growth in an environment.

Abstract: A method and system for analyzing a sample potentially comprising a target, comprising: (i) activating one or more components of the sample analysis system in preparation for a sample, wherein the sample analysis system comprises a heating element, a centrifuge, a thermocycler, an imager, a user interface, and a processor; (ii) obtaining a sample potentially comprising a target; (iii) adding the obtained sample to a sample preparation receptacle, the sample preparation receptacle comprising one or more reagents configured to maximize success of the sample analysis; (iv) heating the sample preparation receptacle, with the added sample, in the heating element of the sample analysis system at a first temperature for a first heating period of time; (v) centrifuging the sample preparation receptacle, after heating, for a first period of time using the centrifuge of the sample analysis system; (vi) transferring at least a portion of the sample, after centrifugation, from the sample preparation receptacle to a sampl

Abstract: A specimen treating and measuring system according to the present invention comprises: a treatment part having a plurality of lanes for carrying out parallel processing of a plurality of specimens, and mounting cartridges in each of the plurality of lanes; a cartridge storing unit storing a plurality of types of cartridge to be used for different processes corresponding to the plurality of specimens; a sample storing unit storing and conveying sample tubes containing the plurality of specimens; a pickup unit transferring each of the plurality of types of cartridge to each lane, and transferring each of the plurality of specimens from the sample tubes to each of the plurality of lanes; and a control unit controlling the transfer of the plurality of types of cartridge and the transfer of the plurality of specimens, performed by the pickup unit.

Abstract: The present disclosure relates to a cuvette box conveying device, a cuvette box conveying method, and a sample analyzer. The cuvette box conveying device includes a frame. The frame includes a new box placing position and a waste box recycling position. The new box placing position and the waste box recycling position are arranged on different layers in the direction of Z axis, so as to reduce the space occupied by the new box placing position and the waste box recycling position on an XY plane. In the cuvette box conveying device, the space occupied by the new box placing position and the waste box recycling position on the XY plane is reduced by arranging the new box placing position and the waste box recycling position on different layers in the direction of Z axis.

Abstract: A sample container carrier, a laboratory sample distribution system comprising such a sample container carrier and a laboratory automation system comprising such a laboratory sample distribution system are presented.

Abstract: The present invention relates to novel modules for transferring magnetic beads, an automated system comprising the same and a method for extracting nucleic acids using the same. The specifically designed magnet module and cover module of the present invention can be employed in the automated liquid handling apparatus by means of pre-existing moving modules (e.g., pipettor module) of the apparatus. The present invention enables a bead transfer-type method for extracting nucleic acids to be performed in an automated manner on the automated liquid handling apparatus. The present invention provides advantages of higher level of automation, more reduced cost and no need for another separate liquid handling apparatus compared to the conventional bead transfer-type method usually performed in the small apparatus designed to be used only for this bead transfer-type method. Also, the present method has the merits of more shortened reaction time compared to the conventional liquid transfer-type method.

Abstract: The object of the invention is to realize an automatic analyzer capable of suppressing an increase in cost without greatly changing the configuration even when the configuration of an inspection system is changed, and reducing burden of an operator. A pin to be fitted into a hole formed in a specimen container conveying unit is formed on a specimen container conveying unit mounting surface at a position below a specimen suction position, and it is possible to make relative positions between components of a specimen dispensing unit and the specimen container conveying unit at the time of a specimen dispensing operation, as well as driving conditions when moving each axis of the specimen dispensing unit identical even when the types of the specimen container conveying unit are different, and it is possible to ensure stability of the same specimen dispensing operation before and after changing the specimen container conveying unit.

Abstract: The object of the invention is to provide an automatic analysis apparatus capable of setting a dispensing mechanism for measuring dispensing accuracy and a dispensing volume, and capable of performing dispensing accuracy measurement without absorbance measurement. Regarding a dispensing accuracy, a dispensing mechanism to be measured is designated. Parameters including a dispensing volume and the like are input. A dispensing operation of the input dispensing volume is automatically performed for each designated dispensing mechanism. A reaction vessel contains, for example, a reagent of the input dispensing volume which has been dispensed by the designated dispensing mechanism. By measuring a weight of the reagent in the reaction vessel, or the like, it is possible to measure the dispensing accuracy of the designated dispensing mechanism.

Abstract: The invention relates to methods and to pipetting apparatuses for detecting at least one performance state of the suction mechanism of a pipetting device used for pipetting.

Abstract: An unmanned aircraft includes a circuit board with an inertia sensor, and a weight block configured to have a flat surface and a recess formed on the flat surface, and a housing assembly configured to form an inner chamber to accommodate the circuit board and the weight block. The circuit board is embedded in the recess by fixedly bonding to the flat surface through adhesion.

Abstract: Using a global optimization, a cycle within a frame buffer including frames corresponding to one or more cycles of query activity sequences is detected. The detection includes creating a plurality of cycle segmentations by recursively iterating through the frame buffer to identify candidate cycles corresponding to cycles of a reference activity sequence until the frame buffer lacks sufficient frames to create additional cycles, computing segmentation errors for each of the plurality of cycle segmentations, and identifying the detected cycle as the one of the plurality of cycle segmentations having a lowest segmentation error. Cycle duration data for the detected cycle is generated. Frames belonging to the detected cycle are removed from the frame buffer. The cycle duration data is output.

Abstract: Motion windows are generated from a query activity sequence. For each of the motion windows in the query activity sequence, a corresponding motion window in the reference activity sequence is found. One or more difference calculations are performed between the motion windows of the query activity sequence and the corresponding motion windows in the reference activity sequence based on at least one criterion associated with physical meaning. Abnormality of the motion windows is determined based on the one or more difference calculations. A standardized evaluation result of the query activity sequence is output based on the detected abnormal motion windows in the query activity sequence.

Abstract: Disclosed is a charge output element, comprising: a support comprising a connecting part; a piezoelectric element, which is an annular structural body and is sleeved on the connecting part, wherein the piezoelectric element is provided with a first deformation groove, and the first deformation groove passes through a side wall of the piezoelectric element to disconnect the piezoelectric element in a circumferential direction; and a mass block, which is an annular structural body and is sleeved on the piezoelectric element, wherein the piezoelectric element is in interference fit with the connecting part and the mass block, and the piezoelectric element, the mass block and the support of the charge output element are in rigid contact with each other. Further disclosed are a method for assembling the charge output element and a piezoelectric accelerometer.

Abstract: A wafer probe station includes a first shielding box, a chuck, a stage, a second shielding box, an electronic testing instrument, a manipulator and a cable. The first shielding box has a first chamber. The chuck is located in the first chamber to hold a device under test. The stage connects to the chuck to move the chuck. The second shielding box is outside the first shielding box and forms a second chamber with the first shielding box. The first and the second shielding boxes shield against an electromagnetic field. The electronic testing instrument is inside the second chamber. The manipulator is outside the first shielding box and has a probe arm penetrating into the first chamber. The probe arm is movable by the manipulator to hold a probe to contact the device under test. The cable connects between the electronic testing instrument and the probe.

Abstract: A prober head to interface an E-testing apparatus to a device under test, which may be an unpackaged die, for example. In some embodiments, the prober head includes an array of conductive pins, each of the pins extending outwardly from a first pin end anchored to a substrate. At least a partial length of each of the pins is coated with a hydrophobic monolayer. The conductive pins may be composite metal wires including a core metal encased by one or more peripheral metal. At a tip of the pins, opposite the first pin end anchored to the substrate, the peripheral metals are recessed from the core metal. In further embodiments, the hydrophobic monolayer is disposed on an outer surface of the peripheral metals, but is substantially absent from a surface of the core metal exposed at the tip.

Abstract: The present invention relates to an analysis of a waveform of a signal. A waveform of the signal is divided into multiple sections and a signal integrity identifier is assigned to each section. Accordingly, a representation of the respective signal integrity identifier may be provided for each section of the waveform. The representation of the signal integrity identifier may comprise an abstract representation, for example a graphical element, an alphanumeric element, a color or even an audio signal.

Abstract: An oscilloscope including an input port for receiving training data including waveforms and corresponding known classifications and a processor for training a plurality of classifiers on the training data. Training includes iteratively applying each classifier to each waveform of the training data to obtain corresponding predicted waveform classifications and comparing the predicted waveform classifications with the known classifications. Classifiers are corrected when predicted waveform classifications does not match the known classifications. Models for each classification are constructed with suggested measurements or actions. Subsequently, live waveform data is captured by the oscilloscope and the classifiers are applied to the live data. When a confidence value for a single classification exceeds a threshold, the waveform data is classified, and suggested measurements or actions are implemented in the oscilloscope based on the classification.