Abstract: The present disclosure relates to the co-expression of an endonuclease with an end-processing enzyme for the purpose of enhanced processing of the polynucleotide ends generated by endonuclease cleavage.

Abstract: The present disclosure relates to the co-expression of an endonuclease with an end-processing enzyme for the purpose of enhanced processing of the polynucleotide ends generated by endonuclease cleavage.

Abstract: The present disclosure relates to the co-expression of an endonuclease with an end-processing enzyme for the purpose of enhanced processing of the polynucleotide ends generated by endonuclease cleavage.

Abstract: Provided is a genome shuffling technology suited to creating a useful next-generation plant body. Genome shuffling is performed using a polypeptide comprising a first polypeptide region for double-stranded DNA breakage activity and a second polypeptide region for intertissue migration activity.

Abstract: An object of the present invention is to provide a method of producing a peptide containing a charged non-proteinogenic amino acid in a cell-free translation system, and the like. The present invention provides a method of producing a peptide containing a charged non-proteinogenic amino acid. It includes a step of expressing a peptide in a cell-free translation system including (i) at least one tRNA to which a non-proteinogenic amino acid having a protecting-group-introduced charged group has been bound and (ii) a nucleic acid that encodes the peptide and contains at least one codon corresponding to an anticodon of the tRNA; and a step of removing the protecting group of the non-proteinogenic amino acid residue contained in the peptide.

Abstract: Described herein is a method for identifying synthetic inducible promoters that have specified induction and/or repression for DNA binding proteins such as an allosteric transcription factor and an inducer molecule. The method includes an in vitro selection from an unselected polynucleotide library comprising a plurality of random degeneracies, and an in vivo selection to produce an induced promoter library. Produced is an induction table, which allows the selection of a promoter with specific induction and/or repression properties. Also included are biosensors containing the synthetic inducible promoters.

Abstract: The present disclosure provides a HTP microbial genomic engineering platform that is computationally driven and integrates molecular biology, automation, and advanced machine learning protocols. This integrative platform utilizes a suite of HTP molecular tool sets to create HTP genetic design libraries, which are derived from, inter alia, scientific insight and iterative pattern recognition. The HTP genomic engineering platform described herein is microbial strain host agnostic and therefore can be implemented across taxa. Furthermore, the disclosed platform can be implemented to modulate or improve any microbial host parameter of interest.

Abstract: The invention generally relates to compositions and methods for designing and producing functional DNA binding effector molecules and associated customized services, tool kits and functional assays. In some aspects, the invention provides methods and tools for efficient assembly of customized TAL effector molecules. Furthermore, the invention relates to uses of TAL effector molecules and functional evaluation of such TAL by, for example, customized assays.

Abstract: The present invention provides for a library of vectors comprising human HC-CDR3 regions of varying length, wherein the diversity of said library is focused on the HC-CDR3 region only and diversity has been optimized such that redundancy is reduced for short HC-CDR3 loops and coverage of HC-CDR3 region variants for longer loop lengths has been increased. The library of the present invention is displayed on phage for selection against target antigens.

Abstract: The invention relates to a method for genotyping individuals, through multiplexing genotyping, when samples carrying different loci of various individuals are pooled and genotyping is performed on this pool.

Abstract: The invention relates to a polyribonucleotide with a sequence that codes a protein or protein fragment, wherein the polyribonucleotide comprises a combination of unmodified and modified nucleotides, wherein 5 to 50% of the uridine nucleotides and 5 to 50% of the cytidin nucleotides are modified uridine nucleotides or modified cytidin nucleotides.

Abstract: The invention relates to the fields of medicine and immunology. In particular, it relates to novel antisense oligonucleotides that may be used in the treatment, prevention and/or delay of Usher syndrome type 2A and/or USH2A-associated non syndromic retina degeneration.

Abstract: The present invention relates to a nucleic acid conjugate represented by the following formula 1: wherein X is an oligonucleotide, L1 and L2 are each independently sugar ligand, and S1, S2 and S3 are each independently a linker.

Abstract: The present invention relates generally to methods for identifying cancer patients with a poor prognosis, and to therapeutic modalities for improving prognosis by combating metastasis and abrogating chemoresistance in cancer cells. Embodiments of the present invention provide an objective means of prognostication regarding the long-term outcome of an incident of cancer, breast cancer in particular. Therapeutic modalities include immunotherapy and anti-sense therapy. Prognosis is determined by measuring the number of copies of the metadherin gene in the patient's cells.

Abstract: The invention relates to antisense polynucleotide agents targeting the LECT2 gene, and methods of using such antisense polynucleotide agents to inhibit expression of LECT2 and to treat subjects having a LECT2-associated disease, e.g., amyloidosis.

Abstract: A method of one or more of, inhibiting motility, migration, dispersal, and metastasis of a cell that expresses an RPTP, which is proteolytically cleaved to form intracellular fragments in the cell includes administering to the cell an amount of an agent effective to inhibit one or more of the catalytic activity and function of the proteolytically cleaved intracellular domain-containing fragments of the RPTP.

Abstract: The invention relates to double-stranded ribonucleic acid (dsRNA) compositions targeting the CD274/PD-L1 gene, and methods of using such dsRNA compositions to inhibit expression of CD274/PD-L1.

Abstract: Modified ribosomes that were selected using a dipeptidyl-puromycin aminonucleoside are used to mediate site-specific incorporation of one or more peptides and peptidomimetics into protein in a cell free translation system. In addition, new fluorescent dipeptidomimetics have been synthesized and incorporated into proteins, as well as modified proteins containing one or more non-naturally occurring dipeptides.

Abstract: The problem to be solved by this invention is to provide a method of gene introduction for a genus Sorghum plant and a method for producing a transformed genus Sorghum plant with a higher efficiency than the conventionally known Agrobacterium method. This invention provides a gene introduction method of a genus Sorghum plant characterized by using a medium with an increased concentration of a nitrogen source and/or an increased concentration of inorganic ions selected from magnesium ion, potassium ion, calcium ion and sodium ion, in a step of preparing a plant tissue material of a genus Sorghum plant, a step of inoculation with Agrobacterium and/or a co-cultivation step. This invention also provides a method for producing a transformed plant using the gene introduction method of this invention.

Abstract: Plants described herein have increased biomass and are more readily digested into fermentable sugars when the plants express increased levels of one or more types of CGR2 and/or CGR3 enzymes.

Abstract: Embodiments of the present invention provide for a transgenic plan, methods of making and DNA constructs for use in the transgenic plant which transgenic plant is capable of modulating its photosynthetic antenna complex composition in response to increases or decreases in light intensity by modulation of the ratio of chlorophyll a to chlorophyll b such that there is an increase in the Chl a/b ratio at high light intensity and a decrease in the Chl a/b ratio at low light intensity versus wild-type plants grown in the same conditions.

Abstract: Methods and compositions for the production of monocot plants with increased starch content in stems are provided. In accordance with the invention, novel promoters and regulatory elements with specific temporal and spatial expression patterns are disclosed together with methods for the production of plants having desirable stem composition at harvest.

Abstract: The present invention relates to a plant having an improved sugar content in fruit compared with its wild type plant, which has a mutant cyclin F-box gene comprising a nucleotide mutation that causes a non-conservative amino acid substitution in the cyclin F-box protein. The present invention also relates to a parthenocarpic plant having a mutant cyclin F-box gene comprising a nucleotide mutation that causes a non-conservative amino acid substitution in the cyclin F-box protein.

Abstract: Compositions and methods related to transgenic high oleic acid/ALS inhibitor-tolerant soybean plants are provided. Specifically, the present invention provides soybean plants having a DP-305423-1 event which imparts a high oleic acid phenotype and tolerance to at least one ALS-inhibiting herbicide. The soybean plant harboring the DP-305423-1 event comprises genomic/transgene junctions having at least the polynucleotide sequence of SEQ ID NO:8, 9, 14, 15, 20, 21, 83 or 84. The characterization of the genomic insertion site of the DP-305423-1 event provides for an enhanced breeding efficiency and enables the use of molecular markers to track the transgene insert in the breeding populations and progeny thereof. Various methods and compositions for the identification, detection, and use of the soybean DP-305423-1 events are provided.

Abstract: The invention provides recombinant DNA molecules useful for providing efficient expression of proteins in transgenic plants, as well as compositions and methods for using the recombinant DNA molecules. In particular embodiments, the invention provides recombinant DNA molecules and constructs comprising sequences encoding transit peptides and operably linked sequences conferring herbicide tolerance.

Abstract: Methods and compositions for deploying refuge seeds together with transgenic crop seeds are provided. The refuge seeds can be non-transgenic seeds of a similar variety to that of the transgenic crop seeds, or the refuge seeds can be a transgenic variety, but in either case lacking a transgenic trait conferring pest protection found in the transgenic crop seeds.

Abstract: Disclosed herein are Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-associated (Cas) 9-based system related compositions and methods of using said CRISPR/Cas9-based system related compositions for altering gene expression and genome engineering. Also disclosed herein are compositions and methods of using said compositions for altering gene expression and genome engineering in muscle, such as skeletal muscle and cardiac muscle.

Abstract: The present invention relates to a producer cell which expresses a tagging protein at the cell surface, such that retroviral vectors produced by the cell are tagged with the tagging protein, wherein the tagging protein comprises: i) a binding domain which binds to a capture moiety ii) a spacer; and iii) a membrane targeting domain such that, when incorporated a retroviral vector, the tagging protein facilitates purification of the retroviral vector from cellular supernatant via binding of the tagging protein to the capture moiety. The present invention also relates to a retroviral vector comprising such a producer cell-derived tagging protein.

Abstract: The present invention provides RNA-guided endonucleases, which are engineered for expression in eukaryotic cells or embryos, and methods of using the RNA-guided endonuclease for targeted genome modification in eukaryotic cells or embryos. Also provided are fusion proteins, wherein each fusion protein comprises a CRISPR/Cas-like protein or fragment thereof and an effector domain. The effector domain can be a cleavage domain, an epigenetic modification domain, a transcriptional activation domain, or a transcriptional repressor domain. Also provided are methods for using the fusion proteins to modify a chromosomal sequence or regulate expression of a chromosomal sequence.

Abstract: The invention relates to a vector comprising: a 5? nucleic acid that is homologous to a genomic sequence 5? of a stop codon of a constitutively expressed gene; an exogenous nucleic acid; a 3? nucleic acid that is homologous to a genomic sequence 3? of the stop codon of the constitutively expressed gene; a translation interruption-reinitiation signal operably linked to the 5? nucleic acid and the exogenous nucleic acid, wherein the translation interruption-reinitiation signal is capable of replacing the stop codon of the constitutively expressed gene.

Abstract: In ethyl alcohol production using the self-fermentation of a microalga, a step of concentrating or collecting an algal body by centrifugal treatment, filtering treatment or the like is made unnecessary or simple to save labor for effort and equipment therefor is saved. The microalga belongs to Chlamydomonas sp., and is a variant strain which has an ability to produce ethyl alcohol under dark and anaerobic conditions and has acquired an ability to proliferate while aggregating. The microalga is proliferated and maintained under dark and anaerobic conditions to generate ethyl alcohol in this method for producing ethyl alcohol.

Abstract: Disclosed herein are enzymes and organisms useful for the dealkylation of products derived from lignin depolymerization, including the conversion of guaiacol or guaethol to catechol or the conversion of anisole to phenol. Methods of converting guaiacol or guaethol to catechol or anisole to phenol using enzymes or organisms expressing the same are also disclosed.

Abstract: A method for producing tagatose comprises: a) performing epimerization of fructose using hexuronate C4-epimerase to obtain an epimerized product comprising tagatose; b) purifying the epimerized product; and c) crystallizing the purified epimerized product. The hexuronate C4-epimerase is an enzyme derived from Thermotoga maritima, Thermotoga neapolitana, Thermotoga thermarum or mutants thereof. The hexuronate C4-epimerase is produced from strains Escherichia coli, Corynebacterum glutamicum, Aspergillus oryzae, or Bacillus subtilis.

Abstract: The invention provides a process for reacting a carboxylic acid ester of the formula (I) R1-A-COOR2??(I), wherein R1 is hydrogen, —CH2OH, —CHO, —COOR3, —CH2SH, —CH2OR3 or —CH2NH2, R2 is an alkyl group, R3 is hydrogen or an alkyl group, and A is a substituted, unsubstituted, linear, branched and/or cyclic alkylene, alkenylene, arylene or aralkylene radical having at least 4 carbons, in the presence of a cell. The process comprises a) contacting the cell with said carboxylic acid ester in an aqueous solution, wherein the cell is a recombinant cell which has reduced activity of a polypeptide comprising SEQ ID NO: 2 or a variant thereof over the wild-type cell.

Abstract: Methods and systems for producing prescribed unit size poly(3-hydroxyalkanoate) (PHA) polymers and copolymers are provided. The methods and systems can employ recombinant bacteria that are not native producers of PHA or lack enzymes to degrade PHA once synthesized, metabolize short to long chain fatty acids without induction, and express an (R)-specific enoyl-CoA hydratase and a PHA synthase, the (R)-specific enoyl-CoA hydratase and PHA synthase having wide substrate specificities. The recombinant bacteria are fed at least one fatty acid substrate that is equal in carbon length to the prescribed or desired unit size of the PHA polymer to be produced. The prescribed unit size PHA that is produced is then isolated and/or purified.

Abstract: The present invention relates to a method for increasing polyhydroxyalkanoates (PHAs) content of waste sludge, by taking the fermentation liquid fermented from waste sludge as a carbon source, and performing ADF domestication process, thereby quickly raising PHAs contentin the waste sludge, in accordance with the method of the present invention, the conventional complicated domestication steps is simplified and a higher content PHAs can be produced, additionally the added carbon source of the present invention is the VFA fermented by the waste sludge, so it's unnecessary to add extra material, therefore including more industrial utilization.

Abstract: Disclosed are techniques and systems for producing microbials having anaplerotic oils that are rich in odd-chain fatty acids, and other beneficial components, at higher concentrations than those present in other natural dietary sources of OCFA, at lower cost, and higher production yield. Such compositions can comprise pentadecanoic and heptadecanoic fatty acids. The techniques described herein include methods for producing and deriving such compositions rich in odd-chain fatty acids from microbials, including microalgae and yeasts/fungi.

Abstract: A method for producing an L-amino acid such as L-glutamic acid is provided. An L-amino acid is produced by culturing a bacterium having an ability to produce an L-amino acid, which has been modified so that the activity of a non-PTS fructose-uptake carrier and the activity of fructokinase are both increased, in a medium containing fructose, and collecting the L-amino acid from the medium or cells of the bacterium.

Abstract: Disclosed herein are methods for improving ethanol production from biomass sources by blocking cellulose from binding to lignin.

Abstract: The invention relates to the use of specific terminal deoxynucleotidyl transferase (TdT) enzymes in a method of nucleic acid synthesis, to methods of synthesizing nucleic acids, and to the use of kits comprising said enzymes in a method of nucleic acid synthesis. The invention also relates to the use of terminal deoxynucleotidyl transferases and 3?-blocked nucleotide triphosphates in a method of template independent nucleic acid synthesis.

Abstract: This invention relates to the use of analogs of purines that have two tautomeric forms, one formally complementary to thymidine, the other formally complementary to cytidine, and analogs of pyrimidines that have two tautomeric forms, one formally complementary to guanosine, the other formally complementary to adenosine. These uses include the polymerase chain reaction amplification of targets having polymorphisms in primer binding sites and ligation.

Abstract: Genetically engineered host animal cells capable of producing glycoproteins having modified glycosylation patterns, e.g., defucosylation and/or monoglycosylation. Such host animal cells can be engineered to express fucosidase, endoglycosidase or both.

Abstract: The present invention describes host cells for reliable, high yield recombinant protein production, including unstable proteins. The present host cell (e.g., a bacterial cell) is deficient in at least one protease (or a subunit of a protease) such as Clp or ClpP. The host cell may also contain an expression vector that encodes a protein or polypeptide for overexpression.

Abstract: Herein is reported a method for the recombinant production of a polypeptide in a eukaryotic cell comprising the steps of (i) cultivating a eukaryotic cell comprising a nucleic acid encoding the polypeptide in a cultivation medium comprising a compound selected from the group consisting of trans-2-methyl 2-pentenoic acid, the broad-spectrum HDAC inhibitor Quisinostat, and the subtype-specific HDAC inhibitor Romidepsin, and (ii) recovering the polypeptide from the cell or the cultivation medium and thereby producing the polypeptide in a eukaryotic cell.

Abstract: The present disclosure relates to genetic markers for discrimination and detection of bacteria causing Edwardsiellosis and Streptococcosis in fish, and a method for discriminating and detecting the bacteria using the same. A genetic marker for discrimination and/or detection of each of Edwardsiella tarda, Streptococcus iniae, Streptococcus parauberis and Lactococcus garvieae, which cause fish diseases, is selected and a peptide nucleic acid and a primer pair, which are specific for the genetic marker, are used to amplify and obtain melting curves having different fluorescences depending on bacterial species. Thus, bacteria that cause fish diseases can be discriminated and whether or not fish would be infected with the bacteria can be detected in a simple, rapid and accurate manner.

Abstract: The present invention concerns a kit and methods for the detection of absence or presence of a ?-lactamase, for example a penicillinase, in a sample comprising or derived from a body fluid taken from a patient suffering from an infection. In some embodiments, the goal of the invention is to rapidly identify infections which can be treated effectively with a penicillin compound. The invention contributes to slowing down the occurrence of resistances to last generation antibiotics. This is achieved by using penicillin compounds for treating infections whenever this can be done efficiently. In brief, the present invention provides a tool for maximizing the use and efficiency of penicillin antibiotics. In other embodiments, the test kit of the invention can be used to detect cephalosporinases (including ESBLs) or carbapenemases.

Abstract: What is disclosed relates to the detection and identification of bacteria of the genera Salmonella and Shigella. It relates more precisely to the methods of microbiology and the culture media used for the detection, identification, isolation and/or analytical investigation of these bacteria. Relating to a method for enrichment and selective culture of bacteria of the genera Salmonella and/or Shigella contained in a biological sample. In the method, some or all of the sample is seeded in/on a culture medium including a nutrient component that favors the development and growth of the bacteria, and includes L-ornithine as a selective agent. It also covers a culture medium suitable for carrying out this method.

Abstract: A method of selectively identifying live microorganisms in a sample involves contacting a sample suspected of containing a live microorganism of interest with a biomolecular precursor labeled with a reactive chemical label. The live microorganism of interest is grown under conditions that promote selective growth of the microorganism to permit the microorganism to utilize the labeled biomolecular precursor to synthesize labeled biomolecules on a cell surface of the live microorganism. Labeled live microorganisms are contacted with a reporter and/or capture element bearing a functional group that reacts with the reactive chemical label. The labeled live microorganism is analyzed to identify it. The method can specifically isolate live microorganisms from dead ones thereby reducing the possibility of false identification of contaminating microorganisms and is able to detect specific strains using tailored metabolites that are specific for a given microorganism.

Abstract: Disclosed is a method for diagnosing a mood disorder or susceptibility to a mood disorder, including depressive disorders and bipolar disorder, from a biological sample taken from a subject. The method includes detecting markers of monoamine oxidase-A (MAO-A) in the biological sample; determining MAO-A concentration from the markers; and correlating the MAO-A concentration in the biological sample to a control group which does not have a mood disorder in order to diagnose or determine susceptibility to the mood disorder in the subject. Also disclosed is a method of detecting peripheral markers of MAO-A for the diagnosis of a mood disorder or susceptibility to a mood disorder. Also provided are polypeptide markers.

Abstract: Methods, reagents, and kits for the reversible immobilization of glycoproteins to a solid support, the release and capture of a glycan portion of the glycoprotein, and the subsequent release and capture of the polypeptide portion of the glycoprotein are provided. The disclosure also provides suitable solid support materials, surface chemistries, and devices for use in the disclosed methods. The methods, reagents, kits, and devices provided herein are useful for the analysis of protein glycosylation, for example, in a diagnostic context, in the context of proteoglycomics, and in the context of producing glycosylated proteins for therapeutic purposes.