Abstract: A microfluidic ion detector for detecting heavy metal ions in liquid and particulate matter from gas samples is described. The microfluidic ion detector includes a sample extraction structure for extracting sample ions from a sample liquid or extracting sample ions from the particulate matter of a gas sample, a separation structure for separating sample ions of different types once extracted, and a detection structure for detecting the sample ions. The microfluidic ion detector also includes a reference reservoir providing a reference ion against which the sample may be calibrated based on the operation of the separation structure. A portable, self-calibrating ion detector may be realized by including the described components on a single substrate.

Abstract: A method of employing an eddy current sensor to perform an inspection of a tube of a steam generator involves accelerating and decelerating the eddy current sensor in a predetermined fashion that substantially reduces the likelihood of damage to the eddy current sensor. The reduction of probe stresses reduces radiological waste and reduces radiation exposure to workers involved in changing damaged probes The recording of an eddy current signal at each of a plurality of equally spaced apart locations along the tube permits the velocity of the eddy current sensor to be varied without compromising the validity of the data that has been recorded. The inspection system employs an encoder that outputs a series of signals as the probe is advanced incremental distances within the tube of the steam generator, and eddy current sensor signals are recorded responsive to at least some of the signals from the encoder.

Abstract: Apparatuses and methods for inspecting a section of piping are disclosed. In one example embodiment, an apparatus includes first and second excitation coils, a plurality of magnetometers, and a data acquisition system. The first excitation coils are disposed at a first axial location and are energized with a first swept frequency waveform and the second excitation coils are disposed at a second axial location and are energized with the swept frequency waveform. The plurality of magnetometers are disposed at an axial location between the first and second axial locations and are positioned to detect magnetic fields generated by eddy currents induced in the section of piping by the first and second excitation coils. The data acquisition system is operatively connected to receive output data from the plurality of magnetometers.

Abstract: A fluid sensor including a sensing area configured to receive a fluid. The fluid sensor includes a transducer and a capacitive sensor. The transducer is configured to output an ultrasonic wave through the fluid. The capacitive sensor includes a capacitive plate configured to reflect the ultrasonic wave toward the transducer.

Abstract: A system and method are described for determining properties of particulates. The device collects particulates on the surface of an oscillating balance and compares the natural resonant frequency of the balance with the particulates with a balance without particulates. In addition, the collected particulates are illuminated with light that is absorbed by the particulates. This causes the balance to heat up, affecting the natural resonant frequency. A comparison of the natural resonant frequencies reveals optical properties of the collected particulates, which may be used to speciate the particulates.

Abstract: A device and method for performing ultra-sound scanning of a substantially cylindrical object. The device comprises a cuff adapted to fit around a circumference of the object, an ultrasound probe mounted about the inner circumference of the cuff and positioned to scan the circumference of the object, and one or more data connections providing control information for the ultrasound probe and receiving scanning data from the ultrasound probe. The probe includes one or more sensors to determine its orientation or location, and this data is used to control the operation of the device and to process data from the array.

Abstract: Method of tuning parameter settings for performing acoustic scanning probe microscopy for subsurface imaging, scanning probe microscopy system, and computer program product. This document relates to a method of tuning a scanning probe microscopy system. The method comprises: a) applying an acoustic vibration signal comprising a first frequency and a second frequency to a sample; b) at a first position of the probe tip, sweeping the first frequency across a first frequency range, and obtaining a first signal; c) at a second position of the probe tip, sweeping the first frequency across at least said first frequency range, and obtaining a second signal; d) analyzing the first and second signals to obtain a difference characteristic dependent on the first frequency. The first and second position are selected such that a subsurface structure of the sample at the first and second position is different.

Abstract: The present disclosure can provide for an ultrasonic welding method for a pair of workpieces. The method can include first pressing an ultrasonic welding stack against a first workpiece in the pair so that the first workpiece comes into contact with a second workpiece in the pair. The method can then provide for initiating a weld phase by outputting energy from the ultrasonic welding stack to the first workpiece. The method can provide for monitoring, with at least one sensor, a sensed parameter. The sensed parameter can be, for example, weld force and/or weld force rate of change. The method can provide for determining whether the sensed parameter has reached a predetermined level. Based on determining that the sensed parameter has reached the predetermined level, the method can provide for ending the weld phase.

Abstract: Generation of feedback for a part production process based on vibrational testing of parts produced by the part production process. A response characteristic may be identified from vibrational data regarding the parts that is correlated to a process variable of the part production process. The response characteristic may relate to a state of the process variable such that identification of the response characteristic may allow for generation of feedback regarding adjustment of a process control. Such response characteristic may relate to a vibrational metric regarding vibrational data and may comprise identifying a trend in data between a plurality of parts. Also presented are approaches to evaluation of parts, including batch evaluation of parts in which collective vibrational data regarding a plurality of parts belonging to a batch are analyzed. The process control aspects may be performed independently or in combination with part evaluation.

Abstract: A method for evaluating noise of a glass run of an automobile door includes: detecting oscillation of the door glass by a sensor for the door glass and then storing the oscillation as an oscillatory wave form of the door glass and detecting oscillation of the frame by a sensor for the frame and then storing the oscillation as an oscillatory wave form of the frame, with the glass run coupling to the automobile door; synthesizing the oscillatory wave form of the door glass and the oscillatory wave form of the frame, picking out relative oscillation of the door glass in relation to the frame and storing the relative oscillation as a synthesized oscillatory wave form; making oscillation corresponding to the synthesized oscillatory wave form by an oscillator and oscillating the door glass with the automobile stopped; and detecting sound of the oscillation by a microphone.

Abstract: A method of performing a photoacoustic physio-chemical analysis is provided. The method includes performing one or more photoacoustic scans on a tissue to generate a plurality of photoacoustic signals. The photoacoustic signals are transformed into a frequency domain to form a power spectra. The method also includes generating a two dimensional (2D) physio-chemical spectrogram from the power spectra. A probe for performing a photoacoustic physio-chemical analysis is also provided.

Abstract: A UT method includes steps of: placing multiple sensors on a test object, the sensors each having multiple elements; connecting the sensors to a UT device such that multiple elements each selected one by one from each of the sensors are set as one group, and the multiple elements belonging to a same group are selectively connected to a common connector of the UT device via a switcher; inputting to the UT device a collection of UT conditions used for the multiple sensors; causing the UT device to perform UT operations sequentially while switching the sensors connected to the UT device with the use of the switcher; and storing UT data in which the sensors used for UT match UT conditions on the basis of the order of execution of UT conditions included in the collection of UT conditions.

Abstract: The present invention relates to a rotary valve and to a chromatography system comprising such a rotary valve. The rotary valve comprising a stator with an inner stator face, and a rotor with an inner rotor face arranged in sealing contact with the inner stator face, the rotor (1) is rotatably movable to a plurality of rotor positions about a rotational axis (RA) relative to the inner stator face (202), wherein the stator comprises ports and orifices and the rotor comprises two or more rotor interconnection paths for selective fluidic interconnection of said orifices with respect to the rotor position and wherein the rotor interconnection paths are arranged to in a rotary position connect the first orifice (S) to the fourth orifice (LF), the second orifice (W) to the third orifice (LE). And in a rotary position connect the eight orifice (SP) to the seventh orifice (PCFP). And in a rotary position connect the eight orifice (SP) to the fifth orifice (TC), the sixth orifice (FC) to the seventh orifice (PCFP).

Abstract: A chromatograph device capable of correct measurement even if the retention times of target components change. This chromatograph device is provided with: a storage unit for storing measurement parameters for a plurality of target components, measurement conditions including measurement parameter switching times for each of two target components eluted in succession, and formulas for determining the switching times from predetermined retention times for the target components; a measurement data accumulation unit; a preceding-measurement-data determination unit for determining, at the time of sample measurement, whether there is preceding measurement data for the same column type, mobile phase type, and flow velocity; and a measurement execution unit for carrying out measurement on the basis of the measurement conditions if there is no preceding measurement data.

Abstract: Certain embodiments described herein are directed to systems and methods that can be used to analyze species in a fluid stream. In some configurations, a sorbent tube effective to directly sample aromatics and/or polyaromatics in a fluid stream is described.

Abstract: A method of measuring a quantity of moisture in an electrode includes at least three steps as follows: disposing an electrode, which is a measurement target sample, inside a container provided with a gas introduction pipe and a gas discharge pipe; heating the electrode by supplying inert gas heated to a predetermined temperature in advance to the inside of the container through the gas introduction pipe, and vaporizing moisture adsorbed to the electrode; and collecting the moisture vaporized from the electrode, together with the inert gas through the gas discharge pipe and determining the quantity of the collected moisture. In addition, a moisture quantity measuring apparatus includes a container that has a gas introduction pipe and a gas discharge pipe, a heating unit, and a moisture quantity measuring unit that collects moisture vaporized from a sample, through the gas discharge pipe and determines the quantity of collected moisture.

Abstract: A process for operating a gas sensor (10), which process includes a calibration of the gas sensor (10). A test gas (16) is drawn in via a pump (12) coupled with the gas sensor (10), whereby the test gas (16) is supplied to the gas sensor (10). Different volume flows are generated by the pump (12) and respective volume flow-dependent measured values (26) are detected by the pump (12). The volume flow-dependent measured values (26) are recorded together with the respective volume flow. A sensor control unit and a computer program functioning as a control program (48) are provided to implement the process.

Abstract: Contaminant cleaning systems and related methods are provided. Exemplary embodiments include a reactive substance generator to produce or transfer reactive substance(s) that react with contaminant(s) on an item into a cleaning chamber. An analysis section can be attached to the cleaning chamber to perform gas analysis on gas samples brought into the analysis chamber that measure reaction byproducts from the reactive substance(s) interaction with the contaminants. An exemplary valve system can selectively couple the reactive substance generator, the analysis section, and the cleaning chamber. An exemplary pumping system, in combination with the valve system, can selectively generate differential pressure/vacuum levels between the reactive substance generator vs cleaning chamber as well as between the cleaning chamber and analysis section. For example, the analysis chamber can be configured to have a higher vacuum than the cleaning chamber to facilitate passage of gas test samples into the analysis chamber.

Abstract: A processor-implemented method receives a plurality of pollution measurement data, a plurality of geodetic data, and a plurality of meteorological data associated with a geographical area. The processor-implemented method generates a grid of the geographical area, whereby the generated grid virtually divides the geographical area into a plurality of cells. The processor-implemented method associates the received plurality of measurement data, the received plurality of geodetic data, and the received plurality of meteorological data with a cell in the generated grid. The processor-implemented method determines a pollutant contribution value of the cell based on the pollution measurement data associated with the cell and a physical contribution of a plurality of pollutants. The processor-implemented method identifies the pollution source based on a paired comparison of the plurality of cells and displays the location on the geographical area associated with the identified pollution source.

Abstract: An air monitoring system for analysing an air sample including: a duct arranged such that the air sample flows therethrough from an inlet to an outlet; a relative humidity sensor arranged to measure a relative humidity of the air sample; a heating module including a first heater arranged to heat the air sample; a temperature sensor arranged to measure a temperature of the air sample; a controller arranged selectively to operate the first heater if the measured relative humidity is above an upper threshold; and an analysis module including at least one chemical analysis transducer providing an output corresponding to a presence or concentration of a predetermined substance in the air sample.

Abstract: A system and method are disclosed for determining a constituent and/or concentration of a solid dissolved in a liquid. In one embodiment, a method is provided to interrogate and identify a liquid in a medical container.

Abstract: A wipe for detecting the presence of an explosive substance is composed of an absorbent or adsorbent substrate and a chemical detection solution impregnated within the substrate. In one embodiment the chemical detection solution includes a combination of reagents operable, when contacted with a particular explosive substance to undergo a chemical reaction or a series of chemical reactions to produce a compound having a visible color. In another embodiment, the chemical detection solution includes a redox color indicating agent that is operable to exhibit a color change upon reacting with the explosive substance.

Abstract: The disclosed apparatus, systems and methods relate to estimating the rate of biodegradation of contaminants in the ground by measuring thermal gradients in the vadose zone where heat is produced by biodegradation reactions, and averaging over a full seasonal period, such as one year or one seasonal cycle, in which the groundwater temperatures and surface temperatures vary in a cyclical manner. Exemplary embodiments correct for the delay required by the heat being produced in the ground to reach the locations where the temperature gradients are measured, and also cancel out the signal noise caused by the changing surface and groundwater temperatures. In further embodiments, a mathematical model is provided to test the validity of the invention on two example sites.

Abstract: A soil analyzing device includes: a sensor having an excitation coil and a detection coil; a measurement unit that generates an excitation signal to be input to the excitation coil, and processes a detection signal output from the detection coil; a storage unit that stores data concerning the correlation between a quantitative value of the soil fertility characteristics including CEC of two or more types of the soil having different components and an estimated value of the soil fertility characteristics including CEC found from the processed detection signal measured by the sensor and the measurement unit; and an estimation unit that estimates the soil fertility characteristics based on the detection signal generated by allowing the sensor to apply the alternating magnetic field to the soil to be analyzed and processed by the measurement unit, and by using the data stored in the storage unit.

Abstract: Soil moisture monitoring systems and methods for measuring mutual inductance of area of influence using radio frequency stimulus are disclosed herein. An example method includes receiving a first inductance frequency sample from a first oscillator circuit, the first oscillator circuit being coupled with one or more reference inductors; receiving a second inductance frequency sample from a second oscillator circuit, the second oscillator circuit being coupled with one or more mutual inductors that sense soil moisture inductance in an area of soil; receiving a temperature reading from a thermometer, the temperature reading being obtained when the first inductance frequency sample and the second inductance frequency sample were obtained. The example method further includes transmitting the first inductance frequency sample, the second inductance frequency sample, and the temperature reading to a receiver.

Abstract: The present invention provides a method for testing an allergy capable of rapidly and highly accurately testing an allergic reaction. The method can determine whether or not a patient has an allergy or whether or not an agent that may be allergenic to a patient has an allergenicity (an allergic reactivity) in the patient. The method may comprise the steps of causing migration of leukocytes separated from a healthy human or cells of an established cell line with a chemotactic factor contained in a sample such as body fluid or blood of the patient to be tested or a sample stimulated with the agent that may be allergenic to the patient and analyzing the cell kinetics such as migration velocity, migration distance, and migration direction.

Abstract: The present invention provides a method for testing an allergy capable of rapidly and highly accurately testing an allergic reaction. The method can determine whether or not a patient has an allergy or whether or not an agent that may be allergenic to a patient has an allergenicity (an allergic reactivity) in the patient. The method may comprise the steps of causing migration of leukocytes separated from a healthy human or cells of an established cell line with a chemotactic factor contained in a sample such as body fluid or blood of the patient to be tested or a sample stimulated with the agent that may be allergenic to the patient and analyzing the cell kinetics such as migration velocity, migration distance, and migration direction.

Abstract: An object of the present invention is to provide a blood test kit of which a component in a small volume of blood can be mailed in a safe state by controlling the influence of the outside temperature during the mailing, and a highly accurate blood analysis method in which coefficient of variation of a measurement value is reduced. According to the present invention, a blood test kit including a diluent solution for diluting a blood sample; a separating means for recovering blood plasma components from the diluted blood sample; a container for storing the blood plasma components recovered from the diluted blood sample; and a cold insulation means for cold-insulating the container, is provided.

Abstract: In the various illustrative embodiments herein, test devices are described with opposing sensor arrays and same side contacts.

Abstract: Endotoxin is detected by electrochemical measurement. A detection device 15 of endotoxin detects endotoxin contained in a fluid to be tested (for example, a dialysate) specifically in the following manner. First, the fluid to be tested is brought into contact with an adsorbent 17 that adsorbs endotoxin to cause the adsorbent 17 to adsorb the endotoxin. Then, a basic solution is brought into contact with the adsorbent 17 to desorb the endotoxin. Electrochemical measurement of the basic solution containing the desorbed endotoxin is performed. The endotoxin can be detected by the electrochemical measurement with high sensitivity at low cost.

Abstract: Means that enables monitoring of an anticancer effect of an anti-CD4 antibody or an anticancer drug targeting an immune checkpoint is disclosed. The method for testing a therapeutic effect of a cancer therapy of the present invention is a method for testing a therapeutic effect of an anticancer drug comprising as an effective ingredient an anti-CD4 antibody or an anticancer drug targeting an immune checkpoint, which method comprises investigation of expression of (1) at least one immune checkpoint receptor, (2) CD8, and (3) at least one cell surface molecule selected from the group consisting of CD44 and CD45RO, on T cells using a sample derived from a patient who received the anticancer drug. Induction of a T cell population which is positive for the immune checkpoint molecule (1) and positive for CD8, and which shows high expression of CD44 and/or high expression of CD45RO, indicates that said anticancer drug is producing a therapeutic effect in said patient.

Abstract: The present invention relates to a use of leucine-zipper protein for diagnosis or treatment of fatty liver. According to the present invention, it could be confirmed that a particular fragment present at the leucine-zipper protein, especially, the N-terminal region thereof, plays an important role in the lipid metabolism in liver tissue by regulating transcriptional activity of Apolipoprotein A4, and therefore, the protein of the present invention or a fragment thereof can be utilized as a target for diagnosis, prevention, or treatment of fatty liver.

Abstract: Existence of human trophoblast stem (hTS) cells has been suspected but unproved. The isolation of hTS cells is reported in the early stage of chorionic villi by expressions of FGF4, FGFR-2, Oct4, Thy-1, and stage-specific embryonic antigens distributed in different compartments of the cell. hTS cells are able to derive into specific cell phenotypes of the three primitive embryonic layers, produce chimeric reactions in mice, and retain a normal karyotype and telomere length. In hTS cells, Oct4 and fgfr-2 expressions can be knockdown by bFGF. These facts suggest that differentiation of the hTS cells play an important role in implantation and placentation. hTS cells could apply to human cell differentiation and for gene and cell-based therapies.

Abstract: The invention relates to a method for predicting the clinical response to a therapy based on the administration of mesenchymal stem cells (MSCs) in a patient suffering from an immune-mediated inflammatory disease. The invention also relates to methods of personalised medicine as well as to therapeutic uses of MSCs in a patient suffering from an immune-mediated inflammatory disease.

Abstract: A method for characterizing extracellular vesicles at an individual level is described. It comprises obtaining a sample comprising extracellular vesicles to be characterized and functionalizing the extracellular vesicles with plasmonic nanoparticles or a plasmonic coating. The method further comprises irradiating the individual extracellular vesicles with a laser beam and detecting a surface enhanced Raman spectroscopy signal from said individual extracellular vesicle.

Abstract: A method for immobilizing an analyte-recognizing molecule (1) on a surface (2?) functionalized with chemical groups Y1 suitable for reacting with a chemical group X2 of a coupling molecule (7) to form a reaction product comprising a chemical group Y2 suitable for reacting with the analyte-recognizing molecule (1), the method comprising the steps of: a) Providing the functionalized surface (2?), b) Contacting the functionalized surface (2?) with a solution (6) comprising simultaneously: i) The coupling molecule (7), and ii) The analyte-recognizing molecule (1).

Abstract: Disclosed herein are formulations, substrates, and arrays. Also disclosed herein are methods for manufacturing and using the formulations, substrates, and arrays. Also disclosed are methods for identifying peptide sequences useful for diagnosis and treatment of disorders, and methods for using the peptide sequences for diagnosis and treatment of disorders, e.g., celiac disorder. In certain embodiments, substrates and arrays comprise a porous layer for synthesis and attachment of polymers or biomolecules.

Abstract: A microfluidic device for conducting a fluid assay includes an injection-molded (or “molded”) fluidics layer having at least one microfluidic channel configured to allow assay fluids to flow there-along, the channel having channel side walls, a channel bottom, and a channel 3D geometry, and the fluidics layer being made from injection-molded liquid silicone (or PDMS). Having the fluidics layer made from injection molded liquid silicone enables smaller-sized channel features, such as microfluidic valves and pistons, smaller channel dimensions and spacing (providing smaller device footprint, higher device capacity and other benefits), and various geometries for the channels and channel features.

Abstract: The present invention relates to methods and systems for testing for the presence of a material such as one or more analyte types within a sample and more particularly, for improved single enzyme-linked immunosorbent assay (sELISA) testing as well as other variants of single-enzyme linked molecular analysis (SELMA). The invention involves flow systems for digital counting of analytes with at least one opening (inlet/outlet). A support with hydrophilic and hydrophobic patches preferably harbours capture probes immobilised on the hydrophilic features. Nano-to-attoliter droplets are formed on the hydrophilic features. A gas phase (called gas phase seal) is applied to prevent/reduce evaporation from the droplets.

Abstract: The present invention relates to methods for identifying markers for systemic lupus erythematosus (SLE) and to the markers identified with the aid of this method, which can differentiate between SLE and other autoimmune diseases on the one hand and between different SLE subgroups on the other hand. The invention also relates to panels, diagnostic devices and test kits which comprise these markers, and to the use and application thereof, for example for the diagnosis, prognosis and therapy control in SLE. The invention also relates to methods for screening and validating active substances for application in SLE subgroups.

Abstract: Presented herein are methods of evaluating cellular activity by: placing a cell population on an area; assaying for a dynamic behavior of the cell population as a function of time; identifying cell(s) of interest based on the dynamic behavior; characterizing a molecular profile of the cell(s); and correlating the obtained information. The assayed dynamic behavior can include cellular activation, cellular inhibition, cellular interaction, protein expression, protein secretion, cellular proliferation, changes in cellular morphology, motility, cell death, cell cytotoxicity, cell lysis, and combinations thereof. Sensors associated with the area may be utilized to facilitate assaying. Molecular profiles of the cell(s) can then be characterized by various methods, such as DNA analysis, RNA analysis, and protein analysis.

Abstract: There are provided methods, and devices for assaying for a binding interaction between a protein, such as a monoclonal antibody, produced by a cell, and a biomolecule. The method may include retaining the cell within a chamber having an aperture; exposing the protein produced by the cell to a capture substrate, wherein the capture substrate is in fluid communication with the protein produced by the cell and wherein the capture substrate is operable to bind the protein produced by the cell; flowing a fluid volume comprising the biomolecule through the chamber via said aperture, wherein the fluid volume is in fluid communication with the capture substrate; and determining a binding interaction between the protein produced by the cell and the biomolecule.

Abstract: The present invention is directed to claudin-1-specific peptide reagents, methods for detecting pre-cancer (dysplasia), early cancer and/or cancer using the peptide reagents, and methods for targeting pre-cancerous (dysplastic) cells, and/or cancer cells using the peptide reagents.

Abstract: The disclosure relates to identifying novel therapeutic targets and/or diagnostic and/or prognostic markers by correcting abnormal RhoH expression in a hematopoietic cell.

Abstract: There is provided a biological substance quantitation method of quantitating a biological substance in a sample stained with a staining reagent including a fluorescent particle encapsulating a fluorescent substance, based on a fluorescence of the fluorescent substance. The method includes inputting a fluorescent image representing expression of the biological substance in the sample by a fluorescent bright spot; and quantitating an expression amount of the biological substance based on a fluorescence of the fluorescent bright spot. The biological substance is a nucleoprotein expressed at a cell nucleus. The fluorescent particle binds to the biological substance through a primary antibody which is directed against the biological substance as an antigen.

Abstract: The present disclosure relates to compounds that are useful as near-infrared fluorescence probes, wherein the compounds include i) a ligand that binds to the active site of carbonic anhydrase, ii) a dye molecule, and iii) a linker molecule that comprises an amino acid, amide, ureido, or polyethylene glycol derivative thereof. The disclosure further describes methods and compositions for making and using the compounds, methods incorporating the compounds, and kits incorporating the compounds.

Abstract: Disclosed herein are methods of high-throughput mapping of viral neutralizing antibody epitopes. Also disclosed are in vitro immunoprecipitation-based adenoassociated virus Barcode-Seq-based methods of mapping viral neutralizing antibody epitopes. In some embodiments, a method of high-throughput mapping of viral NtAb conformational epitopes can be utilized, which may comprise HP scanning of mutant viral libraries, immunoprecipitation (IP), and/or next-generation sequencing (NGS) technology. In some embodiments, a method of identifying one or more dominant epitopes in a viral vector may comprise contacting a mutant capsid of a virus with serum from a subject previously exposed to the virus and immunopredpitating serum immunoglobulins from the serum. In various embodiments, the viral vector may be an AAV vector.

Abstract: There is provided herein a method for identifying and/or recovering at least one genetically encoded affinity reagent specific for a target molecule by screening using molecular display in conjunction with the sequencing of positive and negative selection pools from the screen.

Abstract: Provided are methods for determining the presence or absence of donor specific antibodies in a biological sample. The methods include mixing a cellular sample from a donor with a biological sample from a recipient under conditions sufficient for recipient immune antibodies, if present, to bind to donor cell surface antigen (Ag) to form an immune antibody-Ag complex, contacting the mixture with beads comprising an antibody that specifically binds the immune antibody-Ag complex (e.g., the Ag or immune antibody) on a surface thereof, adding under lysis conditions a detectably-labeled antibody that specifically binds the immune antibody-Ag complex bound to the beads, and detecting the presence or absence of the detectably-labeled antibody bound to the immune antibody-Ag complex to determine the presence or absence of donor specific antibodies in the biological sample from the recipient. Systems and kits for practicing the subject methods are also provided.

Abstract: The present invention relates to the identification of chemokine biomarkers predictive of future acute coronary syndromes including unstable angina pectoris (UAP). The present invention also identifies particular chemokines as potential therapeutic targets for intervention in cardiovascular diseases.