Patents Issued in December 24, 2020
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Publication number: 20200399636Abstract: Disclosed is an enclosed magnetic beads-based reaction system for automation of multi-step DNA preparation process involving DNA purification, modification and amplification. It is especially suitable for making DNA library preparation for next-generation sequencing. It uses modules to perform steps of the multi-step reaction and uses magnetic beads to carry DNA to travel through modules to accomplish the multi-step process. The operation can be fully automated, saving time and repetitive hands-on operations and reducing human errors. Using the enclosed reaction system allows parallel processing of different samples and avoids cross-contamination.Type: ApplicationFiled: June 20, 2020Publication date: December 24, 2020Inventors: Yan Wang, Lili Chen
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Publication number: 20200399637Abstract: The present disclosure provides genetically modified plants, plant cells and plant tissues that show reduced lignin content as compared to a control plant which was not genetically modified. In addition, the disclosure provides methods of regulating lignin content in a plant. The disclosure also provides methods of producing bioproducts using the genetically modified plants of the instant disclosure.Type: ApplicationFiled: June 19, 2019Publication date: December 24, 2020Inventors: Meng Xie, Jin-Gui Chen, Lee E. Gunter, Sara S. Jawdy, Wellington Muchero, Gerald Tuskan, Jin Zhang
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Publication number: 20200399638Abstract: Antisense oligonucleotides capable of preventing or reducing exon 73 inclusion into the human COL7A mRNA are characterized in various ways: (a) the oligonucleotide's sequence includes at most two CpG sequences; (b) the oligonucleotide has a length of no more than 24 nucleotides; (c) the oligonucleotide is capable of annealing to the (SRp40/SC35 binding/ESE) element in exon73. These oligonucleotides can usefully be oligoribonucleotides with modified internucleosidic linkages e.g. phosphorothioate linkages.Type: ApplicationFiled: January 17, 2020Publication date: December 24, 2020Inventors: Elisabeth Marlene Haisma, Marko Potman, Wouter Beumer, Vera Brinks
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Publication number: 20200399639Abstract: The present invention is directed to an aptamer composition comprising at least one oligonucleotide consisting of: deoxyribonucleotides, ribonucleotides, derivatives of deoxyribonucleotides, derivatives of ribonucleotides, and mixtures thereof; wherein said aptamer composition has a binding affinity for one or more bacterial species from the genera Prevotella and Porphyromonas.Type: ApplicationFiled: May 6, 2020Publication date: December 24, 2020Inventors: Sancai Xie, Thomas Glenn Huggins, JR., Cheryl Sue Tansky, Ping Hu, Susan Ellen Forest
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Publication number: 20200399640Abstract: Provided herein are oligonucleotide compositions that inhibit ANGPTL7 and reduce intraocular pressure when administered to an eye. The oligonucleotide compositions contain nucleoside modifications.Type: ApplicationFiled: September 4, 2020Publication date: December 24, 2020Inventors: Omri GOTTESMAN, Shannon BRUSE, Paul BUSKE, Brian CAJES, David LEWIS, David ROZEMA
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Publication number: 20200399641Abstract: The present invention relates to antisense oligonucleotides (oligomers) that are complementary to HTRA1, leading to modulation of the expression of HTRA1. Modulation of HTRA1 expression is beneficial for a range of medical disorders, such as macular degeneration, e.g. age-related macular degeneration.Type: ApplicationFiled: December 30, 2019Publication date: December 24, 2020Inventors: Roberto Iacone, Peter Hagedorn, Susanne Kammler, Soren Ottosen, Sindri Traustason, Heidi Hudlebusch, Lykke Pedersen
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Publication number: 20200399642Abstract: Some embodiments of the methods and compositions provided herein relate to the treatment and amelioration of metastatic tumors and to the prevention of distant metastasis. In some embodiments, a metastatic tumor, such as a melanoma, can be treated by reducing the activity of NOX2 in a cell of a subject. In some embodiments, the activity of NOX2 can be reduced by administering a NOX2 inhibitor, such as histamine dihydrochloride (HDC).Type: ApplicationFiled: January 27, 2020Publication date: December 24, 2020Inventors: Anna Martner, Kristoffer Hellstrand
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Publication number: 20200399643Abstract: Provided herein, inter alia, are p38 mitogen-activated protein kinase inhibitors and methods of treating cancer using p38 mitogen-activated protein kinase inhibitorsType: ApplicationFiled: June 15, 2020Publication date: December 24, 2020Inventors: John J. Rossi, Sorah Yoon
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Publication number: 20200399644Abstract: Methods of treating an adult mammal for an aging-associated impairment are provided. Aspects of the methods include modulating CCR3, e.g., by modulating eotaxin-1/CCR3 interaction, in the mammal in a manner sufficient to treat the mammal for the aging-associated impairment. A variety of aging-associated impairments may be treated by practice of the methods, which impairments include cognitive impairments.Type: ApplicationFiled: April 7, 2020Publication date: December 24, 2020Inventors: Anton Wyss-Coray, Thomas A. Rando, Markus Britschgi, Kaspar Rufibach, Saul A. Villeda
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Publication number: 20200399645Abstract: The present invention relates to a vector, preferably included in a delivery vehicle, comprising no more than 100, preferably no more than 10, restriction sites recognized by the restriction enzymes encoded by each bacterium of a group of bacteria of interest. The invention also relates to the use of said vector, preferably included in a delivery vehicle, as a drug, especially in the treatment of a disease in a patient in need thereof.Type: ApplicationFiled: September 10, 2020Publication date: December 24, 2020Inventors: Antoine DECRULLE, Jesus FERNANDEZ RODRIGUEZ, Xavier DUPORTET, David BIKARD
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Publication number: 20200399646Abstract: Described are expression constructs, cells, and methods of producing proteins in Pichia pastoris.Type: ApplicationFiled: January 10, 2018Publication date: December 24, 2020Applicant: Massachusetts Institute of TechnologyInventors: Kerry R. Love, J. Christopher Love, Charles Whittaker, Joseph Brady, Catherine Bartlett Matthews, Noelle Colant, Neil C. Dalvie
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Publication number: 20200399647Abstract: Disclosed herein are methods of genome-editing transformation that delivers to the cell the RNA-guided endonucleases as well as the required guide RNA, without the need of tissue culture to regenerate the plant. Thus, methods as described herein enable any plant species to have its genome edited in a process that is simple and efficient. This process utilizes genome-editing pollen cells, and delivering the required components for the editing by combining them with cell-penetrating peptides.Type: ApplicationFiled: December 4, 2018Publication date: December 24, 2020Inventors: Marcio Fernando RESENDE, Jr., Kelly Mayrink BALMANT, Kristen LEACH, Matias KIRST, Christopher DERVINIS
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Publication number: 20200399648Abstract: Methods and compositions to modify the fatty acid profiles of seeds and the oils produced therefrom include the modification of four or more alleles of fatty acid desaturases in the genome of a plant. Compositions, polynucleotide constructs, transformed and modified host cells, and plants and seeds exhibiting altered fatty characteristics are provided.Type: ApplicationFiled: March 1, 2019Publication date: December 24, 2020Applicant: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: JOHN D EVERARD, ANTHONY J KINNEY, ZHAN-BIN LIU
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Publication number: 20200399649Abstract: The invention provides recombinant organisms containing a nucleic acid encoding a heterologous glycomolecule that has a low sulfation profile or that is unsulfated. In one embodiment the heterologous glycomolecule is an immunoglobulin molecule. The recombinant organisms have a genetic modification to at least one sulfotransferase gene, such as a deletion, disruption, or other genetic modification. The cells advantageously produce and, optionally secrete, the heterologous glycomolecule. Thus, the invention provides recombinant organisms that provide glycomolecules having a glycosylation profile that is more similar to the glycosylation profile produced in a mammalian cell, and therefore may be safer and more effective for use as a therapeutic in humans or animals. The glycomolecules can be a glycoprotein, glycopeptide, or a glycolipid.Type: ApplicationFiled: September 4, 2020Publication date: December 24, 2020Applicant: Conagen Inc.Inventors: Nicky C. Caiazza, Jun Urano
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Publication number: 20200399650Abstract: The present technology relates to a method for generating crop yield traits. The method comprises identifying a multi-genic system associated with one or more gene of interest, wherein the one or more gene of interest is operatively linked to yield of crop. Identifying at least one combination of genes within the multi-genic system that yields an active multi-protein system of interest and generating mutational diversity on the at least one combination of genes to generate a library. The method further comprises screening the library to identify mutations yielding an improved activity of multi-protein system of interest in an expression system and introducing the screened mutations into the crop.Type: ApplicationFiled: June 19, 2020Publication date: December 24, 2020Inventors: Cedric BOISART, Nicolas CHABOT
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Publication number: 20200399651Abstract: Provided is a plant or plant part comprising a polynucleotide encoding a mutant TriA polypeptide, the expression of the polynucleotide confers to the plant or plant part tolerance to herbicidesType: ApplicationFiled: January 15, 2019Publication date: December 24, 2020Inventors: Doreen Schachtschabel, Eva Hollenbach, Mihiret Tekeste Sisay, Thomas Zierke, Danny Geerdink
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Publication number: 20200399652Abstract: The invention relates to a Solanum lycopersicum plant that is resistant to TBRFV, which plant comprises a QTL on chromosome 11 between SEQ ID NO: 1 and 53. The presence of the QTL on chromosome 11 is identified by use of at least one of the markers selected from the group comprising SEQ ID NOS: 4 to 52. The QTL is as comprised in the genome of a Solanum lycopersicum plant representative seed of which was deposited with the NCIMB under deposit number NCIMB 42882, NCIMB 42885, NCIMB 42887, or NCIMB 42890.Type: ApplicationFiled: May 13, 2020Publication date: December 24, 2020Inventors: Roel Hamelink, Jonathan Kalisvaart, Hamed Rashidi
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Publication number: 20200399653Abstract: Compositions and methods for controlling pests are provided. The methods involve transforming organisms with a nucleic acid sequence encoding an insecticidal protein. In particular, the nucleic acid sequences are useful for preparing plants and microorganisms that possess insecticidal activity. Thus, transformed bacteria, plants, plant cells, plant tissues and seeds are provided. Compositions are insecticidal nucleic acids and proteins of bacterial species. The sequences find use in the construction of expression vectors for subsequent transformation into organisms of interest including plants, as probes for the isolation of other homologous (or partially homologous) genes. The pesticidal proteins find use in controlling, inhibiting growth or killing Lepidopteran, Coleopteran, Dipteran, fungal, Hemipteran and nematode pest populations and for producing compositions with insecticidal activity.Type: ApplicationFiled: July 1, 2020Publication date: December 24, 2020Applicants: PIONEER HI-BRED INTERNATIONAL, INC., E. I. DU PONT DE NEMOURS AND COMPANYInventors: JENNIFER KARA BARRY, DEBORAH CLARK, JAMES J ENGLISH, AZALEA S ONG, ERIC SCHEPERS, JULIE QI, JANET RICE
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Publication number: 20200399654Abstract: The disclosure presents a platform for discovering novel insecticidal proteins from highly heterogeneous environmental sources. The methodology utilizes metagenomic enrichment procedures and unique genetic amplification techniques, which enables access to a broad class of unknown microbial diversity and their resultant proteome. The disclosed insecticidal protein discovery platform (IPDP) can be computationally driven and is able to integrate molecular biology, automation, and advanced machine learning protocols. The platform will enable researchers to rapidly and accurately access the vast repertoire of untapped insecticidal proteins produced by uncharacterized and complex microbial environmental samples. Also presented herein are a group of newly discovered pore-forming toxins (PFT) from a rare class of insecticidal proteins, which were discovered utilizing the insecticidal protein discovery platform.Type: ApplicationFiled: September 1, 2020Publication date: December 24, 2020Inventors: William W. HWANG, Jeffrey Kim, Oliver Liu, Jennifer Shock
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Publication number: 20200399655Abstract: The invention features lentiviral transfer vectors that include heterologous nucleic acids to be introduced into a cell. The lentiviral transfer vector may be characterized by the following features: (a) including a cytomegalovirus (CMV) promoter; (b) including a polynucleotide encoding a partial gag protein that includes a mutated INS1 inhibitory sequence that reduces restriction of nuclear export of RNA; (c) not including a polynucleotide encoding the INS2, INS3, and INS4 inhibitory sequences of gag; (d) not including an SV40 origin of replication and/or an f1 origin of replication; (e) including a cPPT sequence that contains splice site; (f) including an EF1alpha promoter with intact splice donor and acceptor sites; and (g) including hepatitis B PRE with mutation in start codon of X protein ORF.Type: ApplicationFiled: May 22, 2020Publication date: December 24, 2020Inventors: Vladimir SLEPUSHKIN, Dmitriy LUKASHEV
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Publication number: 20200399656Abstract: Disclosed herein are nucleic acid regulatory elements, expression cassettes, expression vectors, and methods for using the nucleic acid regulatory elements, expression cassettes, expression vectors for treating cone cell disorders.Type: ApplicationFiled: December 5, 2018Publication date: December 24, 2020Inventors: Maureen NEITZ, Jay NEITZ
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Publication number: 20200399657Abstract: One disadvantage of adeno-associated virus (AAV) is low gene expression efficiency due to delayed expression of the inserted gene. Provided is a vector plasmid for producing a vector genome to be inserted into an adeno-associated virus (AAV). The vector plasmid is provided with a vector genome cassette as a template of the vector genome, and the vector genome cassette is provided with: (1) an expression cassette including a nucleic acid molecule encoding a target gene and (b) a nucleic acid molecule encoding an expression regulatory region that allows expression of the target gene; and (2) two nucleic acid molecules positioned on both sides of the expression cassette and encoding inverted terminal repeats (ITRs). Also provided is a pharmaceutical composition containing the AVV vector, for treating or preventing an eye disease or disease associated with same.Type: ApplicationFiled: January 16, 2019Publication date: December 24, 2020Inventors: Takashi OKADA, Tsutomu IGARASHI, Asaka SHIOZAWA
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Publication number: 20200399658Abstract: The present disclosure relates to an electroporation device that may include many electroporation units and electroporation systems that can be used in an automated environment, e.g., as one station or module in a multi-station or multi-module cell processing environment.Type: ApplicationFiled: September 4, 2020Publication date: December 24, 2020Inventors: Jorge Bernate, Phil Belgrader, Don Masquelier, Vlorent Morina
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Publication number: 20200399659Abstract: A stable transformant, in which a gene of interest is incorporated into the genome, is simply and efficiently produced. A method for producing a transformant, comprising a step of introducing into a host, linear genome-introduced nucleic acid fragment(s) comprising a gene of interest and a helper plasmid for transformation having a pair of homologous recombination sequences for incorporation of the linear genome-introduced nucleic acid fragment(s), and then selecting a transformant, in which the gene of interest is incorporated into the predetermined position in the host genome and the gene of interest is expressed therein.Type: ApplicationFiled: June 23, 2020Publication date: December 24, 2020Applicant: TOYOTA JIDOSHA KABUSHIKI KAISHAInventors: Toru Onishi, Nobuki Tada
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Publication number: 20200399660Abstract: Provided herein are compounds, compositions, and methods for delivering biomolecules to cells. In particular, the present disclosure provides modified polyamine polymers, including polyamine polymers having fluorinated substituents.Type: ApplicationFiled: June 23, 2020Publication date: December 24, 2020Inventors: Wenhui Zhou, Trish Hoang, Christopher Todd Eggers, Hui Wang, Thomas Machleidt, Brock Binkowski, Poncho Meisenheimer, Frank Fan, Keith Wood
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Publication number: 20200399661Abstract: Provided herein are material and methods for changing gene expression in select sex chromosomes. The materials and methods of the subject invention can be used to produce non-human transgenic animals that produce progeny of a predetermined gender and to generate non-human transgenic animals that produce single-sexed semen.Type: ApplicationFiled: February 26, 2019Publication date: December 24, 2020Applicant: Aggenetics, Inc.Inventor: James WEST
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Publication number: 20200399662Abstract: This invention relates to modified hydroxylases. The invention further relates to cells expressing such modified hydroxylases and methods of producing hydroxylated alkanes by contacting a suitable substrate with such cells.Type: ApplicationFiled: May 11, 2020Publication date: December 24, 2020Inventors: Frances Arnold, Peter Meinhold, Matthew W. Peters, Rudi Fasan, Mike M.Y. Chen
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Publication number: 20200399663Abstract: Methods and systems for isolating a high purity starch stream are provided. The methods provide an initial treatment step in advance of traditional grinding or fractionation operations (such as dry milling or wet milling operations) that facilitate separation of starch from other components of starch-based grains, for example facilitate separation of soft endosperm from other components of corn kernels including hard endosperm. The systems include a first treatment system for separating a high purity starch stream from other components of the grain stream, wherein the system can be configured as a bolt on for traditional milling operations such as wet milling and dry milling operations.Type: ApplicationFiled: September 1, 2020Publication date: December 24, 2020Inventors: Steven Redford, Todd Peterson
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Publication number: 20200399664Abstract: The invention is directed to a process for producing one or more fermentation product in a multi-stage process including an inoculation reactor and at least one bioreactor. The inoculation reactor is fed a C1-containing gaseous substrate containing a reduced amount of hydrogen. The hydrogen is reduced to increase the proportion of CO in the C1-containing gaseous substrate being provided to the inoculation reactor. The inoculation reactor ferments the CO-rich C1-containing gaseous substrate and produces an inoculum, which is fed to at least one bioreactor. The bioreactor receives the C1-containing gaseous substrate, which may or may not contain reduced amounts of hydrogen, to produce one or more fermentation product. By providing a CO-rich C1-containing gaseous substrate to the inoculation reactor, both the inoculation reactor and the subsequent bioreactor(s), are able to have increased stability and product selectivity.Type: ApplicationFiled: September 8, 2020Publication date: December 24, 2020Inventors: Robert John Conrado, Guy William Waters, Matthew Puglisi, Joshua Jeremy Conolly
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Publication number: 20200399665Abstract: The present invention generally relates to the field of biotechnology as it applies to the production of hydroxycinnamic acids using polypeptides having tyrosine ammonia lyase activity. More particularly, the present invention pertains to polypeptides having tyrosine ammonia lyase activity and high substrate specificity towards tyrosine, which makes them particularly suitable in the production of p-coumaric acid and other hydroxycinnamic acids. The present invention thus provides processes for the production of p-coumaric acid and other hydroxycinnamic acids employing these polypeptides as well as recombinant host cells expressing same.Type: ApplicationFiled: July 24, 2020Publication date: December 24, 2020Inventors: Christian Bille JENDRESEN, Solvej SIEDLER, Steen Gustav STAHLHUT, Alex Toftgaard NIELSEN
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Publication number: 20200399666Abstract: Methods for the preparation of bio-based malonic acid and diester derivatives of malonic acid are provided. The disclosed methods produce diester derivatives of malonic acid with fewer impurities, which is useful for many industrial processes. The diester derivatives of malonic acid can be purified from existing sources of malonic acid, or from malonic acid made from a renewable carbon source.Type: ApplicationFiled: February 12, 2019Publication date: December 24, 2020Inventors: Jeffrey A. Dietrich, Eric Steen, Johan van Walsem
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Publication number: 20200399667Abstract: The present application relates to a method for extracting coenzyme Q10 and a phospholipid from a coenzyme Q10 fermentation bacterium powder. The method is characterized in that the fermentation bacterium powder of a coenzyme Q10 production strain is subjected to extraction with a mixed solvent of which the three-dimensional Hansen solubility parameter is between 21 and 23 (J/cm3)1/2 and the hydrogen bonding solubility parameter thereof is between 10 and 12 (J/cm3)1/2. The present invention can efficiently extract two products, namely coenzyme Q10 and a phospholipid, from the coenzyme Q10 fermentation bacterium powder; the process thereof is highly operable, easy to be industrialized, and can provide a product with high purity and yield, having great economic benefit.Type: ApplicationFiled: May 23, 2018Publication date: December 24, 2020Inventors: Shenfeng YUAN, Yang YOA, Yi MIN, Baishan HU, Zhaofeng CHEN, Yong LI, Qichuan LI, Yunxuan LUO, Jinyang ZHANG, Xuguang LI, Shichun TU
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Publication number: 20200399668Abstract: The present invention relates to methods of releasing galactose from legumes using polypeptides having alpha-galactosidase activity. The invention also relates to polypeptides having alpha-galactosidase activity, polynucleotides encoding the polypeptides, nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing the polypeptides. The invention also relates to compositions comprising the polypeptides of the invention and the use of the polypeptides in animal feed.Type: ApplicationFiled: September 8, 2020Publication date: December 24, 2020Applicant: Novozymes A/SInventors: Lone Carstensen, Nikolaj Spodsberg, Morten Gjermansen, Jesper Salomon, Kristian B.R.M. Krogh, Eduardo Antonio Della Pia
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Publication number: 20200399669Abstract: The present invention relates to methods of releasing galactose from legumes using polypeptides having alpha-galactosidase activity. The invention also relates to polypeptides having alpha-galactosidase activity, polynucleotides encoding the polypeptides, nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing the polypeptides. The invention also relates to compositions comprising the polypeptides of the invention and the use of the polypeptides in animal feed.Type: ApplicationFiled: September 8, 2020Publication date: December 24, 2020Applicant: Novozymes A/SInventors: Lone Carstensen, Nikolaj Spodsberg, Morten Gjermansen, Jesper Salomon, Kristian B.R.M. Krogh, Eduardo Antonio Della Pia
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Publication number: 20200399670Abstract: Herein is reported a method for the recombinant production of a polypeptide in a eukaryotic cell comprising the steps of (i) cultivating a eukaryotic cell comprising a nucleic acid encoding the polypeptide in a cultivation medium comprising a compound selected from the group consisting of trans-2-methyl 2-pentenoic acid, the broad-spectrum HDAC inhibitor Quisinostat, and the subtype-specific HDAC inhibitor Romidepsin, and (ii) recovering the polypeptide from the cell or the cultivation medium and thereby producing the polypeptide in a eukaryotic cell.Type: ApplicationFiled: July 8, 2020Publication date: December 24, 2020Applicant: Hoffmann-La Roche Inc.Inventors: Tobias KILLIAN, Markus NEUBAUER
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Publication number: 20200399671Abstract: The purpose of the present invention is to provide a fungus-derived FADGDH that has a direct electron transfer ability. Provided is a fusion protein comprising a fungus-derived FADGDH or a mutant thereof and a cytochrome molecule connected to the N-terminus of the FADGDH or variant thereof.Type: ApplicationFiled: March 8, 2019Publication date: December 24, 2020Inventors: Koji SODE, Youngjea KANG, Kazushige MORI, Katsuhiro KOJIMA, Junko SHIMAZAKI
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Publication number: 20200399672Abstract: The present invention relates to an immunochromatographic analysis device for detecting chikungunya virus, including a sample loading part, a labeling substance retaining part, a chromatographic medium part having a detection part, and an absorption part, wherein the labeling substance retaining part contains a first antibody against the chikungunya virus, including a specific heavy chain variable region and a specific light chain variable region or an antigen-binding fragment thereof, and the detection part contains a second antibody against the chikungunya virus, including a specific heavy chain variable region and the light chain variable region or an antigen-binding fragment thereof, and a third antibody against the chikungunya virus, including a specific heavy chain variable region and the light chain variable region or an antigen-binding fragment thereof.Type: ApplicationFiled: February 8, 2019Publication date: December 24, 2020Inventors: Tatsuo SHIODA, Emi NAKAYAMA, Keita SUZUKI, Hisahiko IWAMOTO
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Publication number: 20200399673Abstract: This document relates to methods and materials involved in assessing and/or treating a mammal having a functional gastrointestinal disorder (FGID) such as irritable bowel syndrome (IBS). For example, methods and materials provided herein can be used for determining if a mammal having a FGID is likely to respond to a particular FGID treatment. This document also provides methods and materials for treating a mammal having a FGID.Type: ApplicationFiled: April 24, 2020Publication date: December 24, 2020Applicant: Mayo Foundation for Medical Education and ResearchInventor: Purna C. Kashyap
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Publication number: 20200399674Abstract: The present disclosure provides a device that includes a base comprising a substrate having a first major surface, a pressure sensitive adhesive adhered to at least a portion of the first major surface, a polymeric cover film coupled to the substrate via the adhesive, a plurality of isolated closed compartments disposed between the substrate and the cover film, and an aqueous liquid disposed in two or more of the closed compartments. The cover film is a composite film comprising ethylene vinyl acetate copolymer, a linear copolymer of ethylene and a higher alkene, and a tackifier. Each compartment of the plurality is defined by a seal that prevents liquid communication with at least one other compartment of the plurality. Methods of using the device for partitioning a sample, for analyzing a sample, and for culturing a microorganism are also provided.Type: ApplicationFiled: September 3, 2020Publication date: December 24, 2020Inventors: Evan D. Brutinel, Kurt J. Halverson
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Publication number: 20200399675Abstract: The present invention provides devices, systems, and methods, for performing biological and chemical assays.Type: ApplicationFiled: August 1, 2018Publication date: December 24, 2020Applicant: Essenlix CorporationInventors: Stephen Y. CHOU, Wei DING
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Publication number: 20200399676Abstract: An isolated polynucleotide encoding a modified luciferase polypeptide and substrates. The OgLuc variant polypeptide has at least 60% amino acid sequence identity to SEQ ID NO: 1 and at least one amino acid substitution at a position corresponding to an amino acid in SEQ ID NO: 1. The OgLuc variant polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the corresponding polypeptide of the wild-type Oplophorus luciferase.Type: ApplicationFiled: September 3, 2020Publication date: December 24, 2020Inventors: Brock Binkowski, Lance P. Encell, Mary Hall, Matthew B. Robers, Michael R. Slater, Keith V. Wood, Monika G. Wood
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Publication number: 20200399677Abstract: Methods for differentially quantifying nucleic acids in a biological sample using differentially methylated loci include forming a digested biological sample in a solution of a methylation-sensitive restriction enzyme to cleave an unmethylated group of nucleic acids, forming a reaction mixture, amplifying the reaction mixture to generate a first signal from the a group of detection probes and a second signal from a second group of detection probes, determining a ratio comprising a first value derived from the first signal to a second value derived from the second signal, and identifying the proportion of nucleic acid in the biological sample originating from the first plurality of nucleic acids.Type: ApplicationFiled: June 24, 2020Publication date: December 24, 2020Inventors: Christopher MacDonald, Lucien Jacky, Aditya Rajagopal
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Publication number: 20200399678Abstract: This invention provides a technology of DNA methylation analysis including: (1) a step of digesting DNA to be analyzed with a restriction enzyme(s) containing methylated cytosine or possibly methylated cytosine in a recognition sequence(s), wherein the recognition site is affected by the methylation; (2) a step of treating the mixture of DNA fragments obtained in the step (1) with ligase to ligate them; (3) a step of determining the base sequence of each DNA constructs included in the mixture of DNA constructs obtained in the step (2); and (4) a step of comparing the base sequence information of each recognition sites and its surrounding sequences, obtained in the step (3), to a known genome sequence; determining whether said each recognition site is not cleaved with said restriction enzyme or cleaved with said restriction enzyme then regenerated by ligation with said ligase; and finally, determining each methylation states of each recognition sites.Type: ApplicationFiled: October 30, 2018Publication date: December 24, 2020Inventor: Naomi YAMAKAWA
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Publication number: 20200399679Abstract: Disclosed herein is a method of utilizing an enzyme in a nucleic acid manipulation process, the method comprising: a) transforming a microorganism with a non-native enzyme; b) inducing expression of the enzyme in the microorganism, thereby producing the non-native enzyme; c) adding the microorganism of step b) directly to a non-naturally occurring nucleic acid manipulation process, wherein the non-native enzyme is not purified from the microorganism prior to addition to the nucleic acid manipulation process; and carrying out the nucleic acid manipulation process using the enzyme. Importantly, this method can be carried out without the need to purify the enzyme from the cell producing it before it is used in the nucleic acid manipulation method. Also disclosed herein is a kit for carrying out a nucleic acid manipulation process, the kit comprising a) a microorganism expressing a non-native enzyme; b) nucleic acids of interest; and c) reagents for use in the nucleic acid manipulation process.Type: ApplicationFiled: November 15, 2018Publication date: December 24, 2020Inventors: Andrew ELLINGTON, Sanchita BHADRA, Jared ELLEFSON, Jimmy GOLLIHAR, Arti POTHUKUCHY, Michelle BYROM, Raghav SHROFF
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Publication number: 20200399680Abstract: The present invention pertains to an in vitro method in which a targeted DNA molecule containing a DNA sequence of interest is enriched by a) general amplification of DNA molecules in a multiple of droplets each containing less than 0.5 target DNA molecule on average (404), b) specific detection of the target DNA molecule in each of the droplets (405), and c) physically selecting droplets containing target DNA molecules (406).Type: ApplicationFiled: June 9, 2020Publication date: December 24, 2020Inventors: Marie Just Mikkelsen, Thomas Kvist
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Publication number: 20200399681Abstract: Sensitive assay methods are described herein that involve (a) capturing circulating cancer cells (e.g., by any method); (b) extracting mRNA and (c) detecting and quantifying each of full-length androgen receptor (AR-FL), androgen receptor variant 7 (AR-V7), and androgen receptor variant 5,6,7 (AR-V567) in parallel digital droplet PCR assays. Additional methods are described herein for detecting and/or isolating circulating cancer cells by selecting for cells that express transferrin receptor.Type: ApplicationFiled: February 22, 2019Publication date: December 24, 2020Inventors: Paraskevi Giannakakou, Ada Gjyrezi, Seaho Kim
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Publication number: 20200399682Abstract: The present invention provides a real-time PCR method that allows in a single step the simultaneous detection of etiological agents of bacterial meningitis, more specifically, Neisseria meningitidis, Streptococcus pneumoniae and Haemophilus influenzae. For this, primers were used to amplify particular regions of the genomes of said bacteria. The presence of bacteria in a sample is indicated by the presence of amplicon, which is detected by means of detection methods appropriate to the PCR methodology employed.Type: ApplicationFiled: February 20, 2019Publication date: December 24, 2020Applicant: FUNDAÇÃO OSWALDO CRUZInventors: IVANO RAFAELLE VICTORIO DE FILIPPIS CAPASSO, ANTONIO EUGENIO CASTRO CARDOSO DE ALMEIDA
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Publication number: 20200399683Abstract: Compositions and methods for the selective extraction of rare molecular species from a mixture containing a predominance of related molecular species, and their subsequent release are provided. Compositions include a segregating probe having a target-specific targeting portion, a protecting portion, a scissile or cleavable site or portion, and a capture portion. Complex formation between the targeting portion and the target rare species results in protection of the cleavable site. Such complexes remain intact through a cleavage step directed to the cleavable site or portion of the segregating probe. While complex formation can occur between a segregating probe and a related, but non-target, species the cleavable site is not protected in such complexes. A subsequent capture step directed to the capture portion selectively captures or segregates the target rare species from the general milieu, which can be removed by washing.Type: ApplicationFiled: February 26, 2019Publication date: December 24, 2020Inventors: William L. Hanna, Alexander Yum
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Publication number: 20200399684Abstract: Provided is a surface having metal regions and an interstitial region having a composition that differs from the metal regions, wherein a continuous gel layer coats the surface across the metal regions and the interstitial regions. Nucleic acids or other analytes can be attached to the continuous gel layer such that a greater amount is attached over the metal regions than over the interstitial region. Also provided are methods for making such surfaces. Methods are also provided for making an array of nucleic acids or other analytes using such surfaces.Type: ApplicationFiled: September 2, 2020Publication date: December 24, 2020Applicant: ILLUMINA, INC.Inventors: Shengrong LIN, Yir-Shyuan WU, Kevin GUNDERSON, John A. MOON
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Publication number: 20200399685Abstract: The present disclosure provides methods and materials for determining if antibiotic resistant H. pylori is present in a sample. The methods may comprise obtaining a threshold level of H. pylori DNA from the sample, amplifying a region of the H. pylori DNA to generate multiple copies of the region of the H. pylori DNA, sequencing the multiple copies of the region of the H. pylori DNA, comparing sequences of multiple copies of the region of the H. pylori DNA to a reference sequence, identifying the presence of a mutation in multiple copies of the region of the H. pylori DNA, and determining a number of the multiple copies of the region of the H. pylori DNA with the mutation, wherein antibiotic resistant H. pylori is present in the sample when the number of the multiple copies of the region of the H. pylori DNA with the mutation is above a predetermined amount.Type: ApplicationFiled: November 16, 2018Publication date: December 24, 2020Inventors: Hongjun ZHANG, Yi ZHOU, Rajarao KAKUTURU