Abstract: A device for detecting a defect on a surface including an optical device (14) suitable for acquiring an image of the surface in a given optical direction, a lighting device (15) comprising a plurality of light sources (16), the light sources (16) being arranged in a hemisphere (20), each light source (16) having an off and an on state, an electronic calculation device (18). The electronic calculation device (18) is able to control the optical device (14) and the lighting device (15) so that the optical device (14) acquires a plurality of images of the surface, different light sources (16) or different combinations of light sources (16) being switched on for each image, the hemisphere (20) having a diameter D smaller than or equal to 50 mm.

Abstract: An attached substance determination method causes a computer to determine whether or not an attached substance is attached to an inspection target object, in which the computer includes at least one processor, and the at least one processor is configured to (a) acquire, as learning data, a spectroscopic image obtained by imaging a first type sample having the attached substance attached to a base with a spectroscopic camera and a spectroscopic image obtained by imaging a second type sample having no attached substance attached to the base with the spectroscopic camera, in which spectroscopic images of a plurality of kinds of the first type samples having different kinds of the bases and different kinds of the attached substances and spectroscopic images of a plurality of kinds of the second type samples having different kinds of the bases are acquired as the learning data, (b) generate, based on the learning data, a determination model with a spectroscopic image of the inspection target object as an input and

Abstract: A method of synchronizing a line scan camera. The method comprises: obtaining line scan data of a region of interest (ROI) of a travelling surface from the line scan camera, the line scan camera being oriented perpendicular to a direction of travel of the travelling surface, the line scan data comprising a plurality of lines; identifying occurrences of a major frequency of a repeated texture on the travelling surface using characterized line scan data for each line in the plurality of lines of the line scan data; determining a period of the major frequency; and changing a line rate of the line scan camera when the determined period is different than a reference period.

Abstract: Methods and systems are disclosed for analyzing one or more images of a textile to determine a presence or absence of defects. In one example, an image of at least a portion of a textile may be obtained and compared to a reference image of a reference textile. Based on the comparison, one or more areas indicative of a height variation between the textile and the reference textile may be determined. An action may be performed based on the one or more areas indicative of the height variation.

Abstract: An adaptor for a turntable of a CT system includes at least two object turntables for at least two objects and a rotation transmission element configured to be coupled to the turntable of the CT system. The two object turntables are coupled to each other using a coupler such that the at least two object turntables rotate simultaneously. The rotation transmission element is coupled to the coupler and configured to transmit a rotation from the turntable of the CT system to the coupler in order to drive the at least two object turntables.

Abstract: When measuring a mass-produced work piece using a measuring X-ray CT apparatus, which is configured to emit X-rays while rotating a work piece that is arranged on a rotary table and to reconstruct a projection image thereof to generate volume data of the work piece, the present invention assigns values to volume data for a predetermined work piece and stores the same as master data; obtains volume data for a mass-produced work piece under identical conditions to the predetermined work piece; measures the volume data and obtains an X-ray CT measured value for the mass-produced work piece; and corrects the X-ray CT measured value for the mass-produced work piece using the master data.

Abstract: A mercury emissions monitor includes a mercury sensor tape configured to be fed in a reel-to-reel manner between first and second tape reels, wherein the mercury sensor tape includes a thin metallic film configured to form an amalgam with detected mercury. A mercury collection unit is configured to receive into a chamber a sample of a gas containing mercury, wherein the mercury collection unit is further configured to permit passage of portions of the mercury sensor tape through the chamber containing the gas sample so that the amalgam is formed with the thin metallic film. A mercury analysis unit includes a total reflection x-ray fluorescence (“TXRF”) system configured to perform a TXRF analysis of the amalgam, wherein the mercury analysis unit is configured to permit passage of the mercury sensor tape within a proximity of an XRF detector of the TXRF system.

Abstract: A system for determining one or more properties of one or more gases. The system comprises sensors configured to measure thermal conductivity and exothermic responses of a sample at multiple temperatures. Sensor responses to exposure to a gas sample at two or more temperatures are compensated and analyzed by a subsystem. The subsystem is configured to determine a thermal conductivity of the gas sample at each of the two or more temperatures and determine at least one component of the gas sample based at least in part on the thermal conductivity value of the sample at each of the two or more temperatures. Related systems and methods of determining one or more properties of a sample are also disclosed.

Abstract: A thermal insulation property measuring device, including a tank, a thermal insulator, a cuboidal frame, a support bracket, and a data collector. The tank includes an upper body, a first lower body, and a second lower body. The thermal insulator includes a first thermal insulation layer, a second thermal insulation layer, a first membrane, a second membrane, and a third membrane. The support bracket includes a trapezoidal support and a transition support. The tank is disposed on the support bracket. The support bracket is disposed in the cuboidal frame. The upper body, the first lower body, and the second lower body are spherical and communicate with each other. A filling tube, a pressure relief tube and a piezometer tube are disposed on the upper body. The first membrane is disposed on the tank. The first thermal insulation layer is coated on the first membrane.

Abstract: A method for identifying and characterizing a condensate entrained in a fluid using time domain analysis and frequency domain analysis to identify individual volume fraction constituents and condensates within a pipe on a real time basis and to measure the volume of the individual volume fraction constituents and condensates flowing through the pipe on a real time basis.

Abstract: A gas sensor includes: a substrate; a first conductor and a second conductor that are disposed on the substrate; an insulating layer; and an adsorbent layer. The insulating layer covers the first conductor and the second conductor, and has a first opening that allows a part of a surface of the first conductor to be exposed therethrough and a second opening that allows a part of a surface of the second conductor to be exposed therethrough. The adsorbent layer contains a conductive material and an organic adsorbent that can adsorb a gas. The adsorbent layer is in contact with the first conductor and the second conductor respectively through the first opening and the second opening.

Abstract: A sensor semiconductor device comprises a transducer which comprises a capacitor with at least two electrodes. The transducer further comprises a polymer which is arranged between at least two electrodes of the capacitor, and a top surface of the transducer. The polymer is able to absorb water and the top surface is arranged such that it is exposed to the environment of the sensor semiconductor device. Furthermore, at least a part of the top surface is superhydrophobic and the sensor semiconductor device is capable of measuring the humidity of the environment of the sensor semiconductor device.

Abstract: Disclosed are devices for determining an analyte concentration (e.g., glucose). The devices comprise a sensor configured to generate a signal associated with a concentration of an analyte and a sensing membrane located over the sensor. The sensing membrane comprises a biointerface layer which interfaces with a biological fluid containing the analyte to be measured. The biointerface layer can comprises a biointerface polymer, wherein the biointerface polymer comprises polyurethane and/or polyurea segments and one or more zwitterionic repeating units. The sensing membrane can also comprise an enzyme layer, wherein the enzyme layer comprises an enzyme and a polymer comprising polyurethane and/or polyurea segments and one or more zwitterionic repeating units. The sensing membrane can also comprise a diffusion-resistance layer, which can comprise a base polymer having a lowest Tg of greater than ?50 C.

Abstract: Methods for fabricating a biochip for detecting or sequencing biomolecules are shown. Such a biochip may for instance include: a base member; a dielectric layer deposited on the base member and having at least two rows of discrete recesses formed thereon; and two or more electrodes sandwiched between the base member and the dielectric layer and running under respective row of discrete recesses, the two or more electrodes separated from each other along lengths thereof by a portion of the dielectric layer; wherein the dielectric layer defines a continuous operation surface above the electrodes and on which the discrete recesses are deposited for detecting or sequencing of biomolecules, when an electric field is applied through the electrodes, a field gradient is created to draw a biomolecule towards a preferred part of the operation surface.

Abstract: A measuring element is disclosed for an ion-sensitive solid-contact electrode for measuring ion activity in a measurement medium. An ion-sensitive solid-contact electrode having such a measuring element and an electrochemical sensor having such a solid-contact electrode are also disclosed. The measuring element can include an ion-sensitive layer arranged to contact a measurement medium when in operation, and conductive to lithium ions; and a single-phase electrically conductive layer, which includes metallic lithium or a lithium-(0)-alloy. A solid-state electrolyte layer can be arranged between the ion-sensitive layer and the electrically conductive layer.

Abstract: Disclosed herein is a method of testing a corrosion resistance of a coated metal material including a surface treatment film on a metal substrate. The method includes: treatment of interposing a water-containing electrolyte material containing water, a supporting electrolyte, and a water penetration enhancer, between a surface of the surface treatment film of the coated metal material and an electrode; holding of the water-containing electrolyte material on the surface of the surface treatment film for one minute to one day; and electrical conduction from the electrode through the water-containing electrolyte material to the coated metal material.

Abstract: An electrophoresis apparatus is generally disclosed for sequentially analyzing a single sample or multiple samples having one or more analytes in high or low concentrations. The apparatus comprises a relatively large-bore transport capillary which intersects with a plurality of small-bore separation capillaries and includes a valve system. Analyte concentrators, having antibody-specific (or related affinity) chemistries, are stationed at the respective intersections of the transport capillary and separation capillaries to bind one or more analytes of interest. The apparatus allows the performance of two or more dimensions for the optimal separation of analytes. The apparatus may also include a plurality of valves surrounding each of the analyte concentrators to localize each of the concentrators to improve the binding of one or more analytes of interest.

Abstract: An ion filter for filtering ions in a gas sample. The ion filter has a first ion channel for filtering ions from a target chemical in the gas sample. The ion filter has a second ion channel for filtering ions from the target chemical in the gas sample. The second ion channel is separated from the first ion channel. A temperature control region is in thermal contact with the first and second ion channels for controlling a difference in temperature between the first and second ion channels. A method of filtering ions from a target chemical in a gas sample is also provided.

Abstract: A measuring device, in particular a flow meter, has a measurement tube for receiving or conveying a fluid, first and second oscillation transducers on a side wall of the measurement tube, and a control device. An operating method has the control device drive the first oscillation transducer chronologically in succession for respective mode-selective excitation of a first and a second oscillation mode of a wave conducted in the side wall of the measurement tube. The second oscillation transducer is driven similarly for a second measurement direction. The excited waves are conducted directly in the side wall or indirectly through the fluid and recorded in the other oscillation transducer, resulting in measurement data for each measurement direction and oscillation mode. Result information relates to a property of the fluid or a state of the measuring device determined from the measurement data for both measurement directions and both respective oscillation modes.

Abstract: A method for concentrating electrically charged objects in a non-Newtonian liquid medium comprises: feeding a sample containing electrically charged objects into a channel having a flow axis, a first transverse cross-section orthogonal to the flow axis, and at least one second transverse cross-section orthogonal to the flow axis, one dimension of the second cross-section being less than the corresponding dimension of the first cross-section; and applying a hydrodynamic flow in a direction of the channel together with the application, in the opposite direction, of an electric field in the channel, thus making it possible to move the electrically charged objects in the channel along the flow axis from the first cross-section to the second cross-section, stop the objects, and concentrate the objects in at least one area upstream from the second transverse cross-section.

Abstract: A liquid chromatography monitoring system comprises a computer or electronic controller comprising computer-readable instructions operable to: (a) draw a fluid into a syringe pump; (b) configure a valve so as to fluidically couple the pump to either a fluidic pathway through a fluidic system or to a plug that prevents fluid flow; (c) cause the syringe pump to progressively compress the fluid therein or expel the fluid to the fluidic pathway, while measuring a pressure of the fluid; (d) determine a profile of the variation of the measured pressure; (e) compare the determined profile to an expected profile that depends upon the fluid; and (f) provide a notification of a sub-optimal operating condition or malfunction if the determined profile varies from the expected profile by greater than a predetermined tolerance.

Abstract: A chromatography detector comprises: a flow cell with a flow path where the sample and a solution flow, the flow cell including outlet tubing that discharges the sample and the solution from the flow path; a connecting member configured to connect the outlet tubing of the flow cell and external tubing outside the flow cell; a wall member including a through-hole; a securing member configured to secure the connecting member to the wall member; and a tray below the securing member. The securing member includes a holder configured to hold the connecting member, an attachment configured to be inserted through the through-hole of the wall member, and a fluid guide formed in such a manner as to, upon the solution leaking out of the connecting member, guide the leaked solution to the tray.

Abstract: A method for analyzing aromatic compounds, and a reagent kit for LC-MS analysis of aromatic compounds. The method includes preparing a diazonium reagent, contacting aromatic compounds in a sample with the diazonium reagent to form an analyte; and measuring an amount or ratio of the analyte. The reagent kit includes a diazonium reagent, wherein the diazonium reagent includes (i) a diazonium salt that contains a diazonium ion; (ii) an amine and nitrous acid; and/or (iii) a nitrite and an acid.

Abstract: Provided is a chromatography mass spectrometry capable of peak detection that can deal with a wide concentration range of a sample component and providing an evaluated value for the result. A plurality of samples having different known concentrations of a component are measured to detect a start point, an apex, and an end point of a peak. Regarding the start point, the apex, and the end point of the detected peak, an evaluated value such as probability is provided as a score to determine a score function. A component having an unknown concentration is measured to detect a start point, an apex, and an end point of a peak. Regarding the start point, the apex, and the end point of the detected peak, the score function is applied to evaluate peak detection results, and a result having a high evaluated value is selected as a peak.

Abstract: Modified thermoplastic hydrocarbon thermoplastic resins are provided, as well as methods of their manufacture and uses thereof in rubber compositions. The modified thermoplastic resins are modified by decreasing the relative quantity of the dimer, trimer, tetramer, and pentamer oligomers as compared to the corresponding unmodified thermoplastic resin polymers, resulting in a product that exhibits a greater shift in the glass transition temperature of the elastomer(s) used in tire formulations. This translates to better viscoelastic predictors of tire tread performance, such as wet grip and rolling resistance. The modified thermoplastic resins impart remarkable properties on various rubber compositions, such as tires, belts, hoses, brakes, and the like. Automobile tires incorporating the modified thermoplastic resins are shown to possess excellent results in balancing the properties of rolling resistance, tire wear, snow performance, and wet braking performance.

Abstract: A remote sensor calibration system remotely calibrates sensors of various types in an automated fashion. The remote sensor calibration system receives sensor output, associated with one or more locations and one or more times, from a sensor and its peer sensor population. The sensor output from the sensor and its peer sensor population is compared to generate a correction factor. The correction factor is transmitted to the sensor and used to calibrate the sensor. Communication will typically occur via one or more wireless links allowing the sensors to be remotely calibrate while maintaining their mobility.

Abstract: The present application is directed to a multi-sensor gas sampling detection system and method for detecting and measuring the radicals in a radical gas stream and includes at least one radical gas generator in communication with at least one gas source. The radical gas generator may be configured to generate at least one radical gas stream which may be used within a processing chamber. As such, the processing chamber is in fluid communication with the radical gas generator. At least one analysis circuit in fluid communication with the radical gas generator may be used in the detection and measurement system. The analysis may be configured to receive a defined volume and/or flow rate of the radical gas stream. In one embodiment, the analysis circuit may be configured to react at least one reagent with radicals within the defined volume of the radical gas stream thereby forming at least one chemical species within at least one compound stream.

Abstract: A method for non-destructive detection of egg freshness based on centroid measurement. A real-time distance between the centroid position of an egg and the top of a small end of the egg is measured, the ratio of the distance to the length of the egg is defined as a centroid ratio, models are respectively established regarding a centroid ratio change and the number of days the egg has been stored, and regarding the centroid ratio change and the egg freshness, and the number of the days the egg has been stored and the egg freshness are predicted by means of the established models.

Abstract: Methods, systems, and apparatus, including computer programs encoded on computer storage media, for molecular content analysis. One of the methods includes receiving molecular content data that indicates a molecular content of a portion of a batch of sludge; determining, by a machine learning module included in the system using the molecular content data and multiple parameters, a predicted value for a property of the portion of the batch of sludge; and providing the predicted value for the property of the portion of the batch of sludge.

Abstract: Systems and methods of measuring properties of water in a water distribution system are provided. An analysis system, according to one embodiment, comprises a plurality of water sensors connected at various points to the water distribution system, each of the plurality of water sensors configured to measure a property of water. The analysis system also includes a computer server configured to communicate with the plurality of water sensors via a network and receive water measurement data from the plurality of water sensors. The computer server comprises a processor, a database configured to store the water measurement data, and a system health monitoring module configured to evaluate the health of the water distribution system to obtain health data. The analysis system further includes at least one client device configured to communicate with the computer server via the network and receive the health data from the computer server.

Abstract: In one aspect, the present subject matter is directed to a system for managing material accumulation relative to a ground engaging assembly of an agricultural implement. The system may include one or more ground engaging tools of the ground engaging assembly, a sensor detecting material accumulation relative to the ground engaging tools, and a controller communicatively coupled to the sensor. The controller may receive an input from the sensor associated with material accumulation relative to the ground engaging tools. The controller may further access data associated with an amount of crop residue present and data associated with a moisture content of soil within the field. Additionally, the controller may determine an accumulation type of the material accumulation based at least in part on the crop residue data and the soil moisture data.

Abstract: A method for measuring a glycated protein in a sample, the method comprising (1) a step of allowing a sample in which a degradation product has been generated from a glycated protein by a protease to react with an oxidoreductase in the presence of an electron mediator to generate a reduced electron mediator; and (2) a step of detecting the reaction state in the step (1) by an electrochemical technique using an interdigitated electrode.

Abstract: A blood coagulation analyzer and analyzing method perform following: (a) preparing a measurement specimen by dispensing a blood specimen and a reagent into a reaction container; (b) emitting light of a plurality of wavelengths to the measurement specimen in the reaction container, the wavelengths comprising a first wavelength for use in a measurement by a blood coagulation time method, and at least one of a second wavelength for use in a measurement by a synthetic substrate method and a third wavelength for use in a measurement by an immunoturbidimetric method; (c) detecting light of a plurality of wavelengths corresponding to the light emitted in (b), from the measurement specimen, by a light receiving element, and acquiring data corresponding to each wavelength; and (d) conducting an analysis based on the data corresponding to one of the wavelengths among the acquired data, and acquiring a result of the analysis.

Abstract: A chip for blood plasma separation includes: (i) a body part, in which a sealed space through which blood can flow is integrally formed and the channel part and a ridge are alternately and continuously formed; (ii) an inflow part, which is disposed at an upper region of the body part into which the blood inflows; (iii) an outlet for discharging blood cells located at one side surface of the body part; and (iv) an outlet for discharging blood plasma located at the other side surface of the body part, in which the ridge is formed discretely, a chip array for blood plasma separation including the chip for blood plasma separation, a device for blood plasma separation including the chip for blood plasma separation and/or the chip array for blood plasma separation, and a method for blood plasma separation using the device.

Abstract: The present invention relates to cell-based methods for screening body fluids or tissues for factors that prevent cellular uptake of lysosomal enzymes, including neutralizing factors such as neutralizing antibodies, that arise as a result of lysosomal enzyme replacement therapy.

Abstract: A multiplexed method of monitoring immune-cell responses to different ligands using a micropillar/microwell plate platform is disclosed. The method may include dispensing immune cells onto at least one micropillar chip and inserting the at least one micropillar into at least one microwell on a microwell chip, in which the at least one microwell contains at least one test compound. The method may also include immobilizing antibodies onto at least one micropillar on a micropillar chip and inserting the at least one micropillar into at least one microwell on a microwell chip, in which the at least one microwell contains a test compound. The method may also include treating the micropillar chip with at least one reactive polymer.

Abstract: Disclosed herein are methods of detecting ascorbic acid in a urine sample from a subject, including contacting at least a portion of the urine sample with a test strip including a reagent pad and detecting whether ascorbic acid is present in the urine sample by measuring an intensity of color on the reagent pad, wherein a reduction in the intensity of color on the reagent pad indicates the presence of ascorbic acid.

Abstract: Provided is a method that utilises linear dichroism (LD) to identify the presence of a target molecule (L) in a sample. The method comprises providing an alignable scaffold (20), preferably biomolecular fibre M13, comprising a first binding region and having a high aspect ratio of greater than 5:1, providing a substrate (e.g. a substantially spherical non-alignable moiety (12)) comprising a second binding region which binds the first binding region in the absence of the target molecule in such a way that the LD signal of the alignable scaffold is reduced or minimised relative to the unbound and aligned scaffold, wherein one of the first and second binding regions is a receptor capable of binding the target molecule, exposing the substrate-bound scaffold to the sample such that binding of the target molecule, if present, to the receptor releases the scaffold from the substrate, and measuring the LD signal of the scaffold before and after exposure to the sample.

Abstract: The present disclosure relates to, inter alia, devices, systems, and methods for use in the magnetic separation of biological entities from fluid samples. This device includes a magnetic separation chamber configured to receive a fluid sample for magnetic separation, where the magnetic separation chamber includes at least two magnets mounted on the surface or in the wall of the magnetic separation chamber. The device also includes a force provider configured to move the magnetic separation chamber in a side-to-side motion to mix and/or magnetize the fluid sample. In one embodiment, the magnetic separation chamber is in a form of a sleeve and comprises a substantially central channel for loading a vessel containing the fluid sample therein. The systems and methods of the present disclosure involve the use of this device to separate biological entities from fluid samples.

Abstract: Materials and methods for the design of hybrid materials comprising a conducting matrix, organic modifiers/linkers and modifying molecules.

Abstract: Embodiments include disposable sensor elements, systems including the same and related methods. In an embodiment, a disposable sensor element is included having a substrate and a first measurement zone comprising a plurality of discrete binding detectors. The first measurement zone can define a portion of a first gas flow path. In some embodiments the disposable sensor element can further include a second measurement zone, separate from the first measurement zone. The second measurement zone can include a plurality of discrete binding detectors. The second measurement zone can be disposed outside of the first gas flow path. Other embodiments are also included herein.

Abstract: A system for measuring analyte concentrations has porous-walled nanocontainers containing multiple magnetic nanoparticles, the magnetic nanoparticles coated with a selective binder that is analyte-responsive and binds a the analyte, an indicator substance releasable from the selective binder by the analyte, or an indicator substance cleavable by the analyte, apparatus for exposing the nanocontainers to a fluid potentially containing the analyte, and magnetic spectroscopy of Brownian motion sensing apparatus for detecting agglutination of the nanoparticles or binding of analyte to the nanoparticles.

Abstract: A sample analysis substrate includes a substrate; a first holding chamber; a reaction chamber; a first flow path having a first opening and a second opening respectively connected with the first holding chamber and reaction chamber; a main chamber; a second flow path having a third opening and a fourth opening respectively connected with the reaction chamber and the main chamber; and a magnet accommodation chamber capable of accommodating a magnet. The first opening is located closer to a rotation shaft than the second opening. The second opening is located closer to the rotation shaft than the third opening. The magnet accommodation chamber is located at a position at which, in the case where the magnet is accommodated in the magnet accommodation chamber, the magnet captures magnetic particles in the main chamber. The sample analysis substrate is rotatable to transfer a liquid.

Abstract: The present invention relates to a functional, easily automatable assay for establishing a heparin-induced thrombocytopenia (HIT). What is measured is the secretion of PF4 (platelet factor 4) from activated thrombocytes.

Abstract: Provided are methods of predicting responsiveness of a subject having an inflammatory bowel disease (IBD) to a tumor necrosis factor (TNF)-alpha inhibitor, by analyzing a frequency of at least one subpopulation of immune cells in a tissue biopsy of the subject. Also provided are methods of selecting a treatment for a subject and kits for determining responsiveness of the subject to treatment with a TNF-alpha inhibitor.

Abstract: A spectral reflectance imaging device for detecting nanoparticle exosome biomarker targets includes an illumination source that illuminates a substrate with a plurality of separate wavelengths of incoherent light. The substrate includes an oxide layer and a binding agent to selectively bind nanoparticle exosome biomarker targets to the substrate. An imaging device bindings the light reflected from or transmitted through the substrate and an image processing system detects the nanoparticle exosome biomarker targets a function of the change in reflective properties of the substrate.

Abstract: The present invention relates to methods for predicting the probability of a treatment response of a human cancer patient to an immune checkpoint blocker treatment e.g. with anti PD-1, and to methods for predicting the probability of survival of a human cancer patient following an immune checkpoint blocker treatment, and to apparatuses and kits which can be used in these methods.

Abstract: Disclosed herein are photodegradable hydrogels and associated kits for selectively capturing and releasing cells. The hydrogels result from cross linking in the presence of a photoinitiator (1) a macromer having a polymeric backbone structure, a photo labile moiety, and a first linking moiety, and (2) a cell-binding moiety having a second linking moiety. These two components are cross-linked by a polymerization reaction of the linking moieties to form a photodegradable hydrogel incorporating the cell-binding moiety within the hydrogel. Also disclosed are methods of making the hydrogels, and methods of using the hydrogels for selectively capturing and releasing cells and for detecting cells in a fluid. Such methods can be used to detect the presence and quantity of certain rare cell types in a biological fluid.

Abstract: The present invention provides biomarkers for sensitive, specific, accurate and quantitative diagnosis and assessment of chronic hypoxia. In particular, the present invention provides 2-hydroxyglutarate as a biomarker that is differentially produced in chronic hypoxia. Furthermore, embodiments of the invention are able to differentiate between chronic and acute hypoxia. Assays for levels of 2-hydroxyglutarate may be used alone or in conjunction with additional biomarkers of hypoxia to increase the precision of analysis. In particular embodiments of the invention, the level of 2-hydroxyglutarate and at least one second biomarker may be assayed to generate a hypoxic profile that can be compared to a reference or control profile, thereby diagnosing a subject as normoxic, chronically hypoxic, or acutely hypoxic.

Abstract: The present invention relates to methods and compositions for monitoring, diagnosis, prognosis, and determination of treatment regimens in subjects suffering from or suspected of having a renal injury.