Abstract: This disclosure provides methods, systems, compositions, and kits for the multiplexed detection of a plurality of analytes in a sample. In some examples, this disclosure provides methods, systems, compositions, and kits wherein multiple analytes may be detected in a single sample volume by acquiring a cumulative measurement or measurements of at least one quantifiable component of a signal. In some cases, additional components of a signal, or additional signals (or components thereof) are also quantified. Each signal or component of a signal may be used to construct a coding scheme which can then be used to determine the presence or absence of any analyte.

Abstract: An affinity reagent, having: (a) a retaining component such as a structured nucleic acid particle; and (b) one or both of (i) one or more label components attached to the retaining component, and (ii) one or more binding components attached to the retaining component.

Abstract: Provided herein are methods for spatial analysis using targeted RNA depletion.

Abstract: Provided herein is a method for imaging RNA transferred from a sample to a substrate. The method may include placing a sample comprising cells on a substrate, transferring RNA from the sample onto the substrate to produce an RNA blot in which the RNA is immobilized on the substrate, removing the sample from the substrate, hybridizing the RNA blot with a set of oligonucleotides that hybridize to different sites in the same RNA species, and reading the blot to obtain an image showing the binding pattern of the hybridized oligonucleotides.

Abstract: Provided herein are products and methods for detecting analytes using polymers that bind to such analytes and thereby undergo a conformational change or contribute to a newly formed complex.

Abstract: Provided herein are quantitative assays to evaluate duplex unwinding efficiency or strand exchange efficiency during isothermal amplification.

Abstract: The present invention relates to a method for detecting nucleic acids by fluorescent PCR. The method combines primer-activated polymerization reaction and specific fluorescence-labeled probe, which detects the target nucleic acid with high selectivity and high specificity.

Abstract: The present disclosure provides compositions of matter, methods and instruments for nucleic acid-guided nickase/reverse transcriptase fusion editing in live cells. Editing efficiency is improved using fusion proteins (e.g., the nickase-RT fusion) that retain certain characteristics of nucleic acid-directed nucleases (e.g., the binding specificity and ability to cleave one or more DNA strands in a targeted manner) combined with reverse transcriptase activity. Editing cassettes are employed, comprising a gRNA and a repair template where the 3? end of the repair template is protected from degradation.

Abstract: This patent application relates generally to mixture deconvolution systems and methods for identifying DNA profiles. Various embodiments of the present invention concern the deconvolution of unknown DNA profiles in a two-person DNA mixture into two DNA profiles. Deconvolution methods isolate distinct DNA profiles from a DNA mixture without the need to match against DNA reference profiles. Various embodiments include a mixture deconvolution pipeline that involves a series of mathematical steps and machine learning algorithms to achieve the desired performance and decision-support outputs. Various embodiments enable distant familial matching to existing investigative genetic genealogy (IGG; also known as forensic genetic genealogy (FGG)) databases. This capability enables the generation of investigative leads from unresolved casework samples (i.e., DNA mixtures) by identifying possible genealogical relationships to one or more person(s) of interest.

Abstract: Disclosed herein in are methods and systems for determining genetic variants (e.g., copy number variation) in a polynucleotide sample. A method for determining copy number variations includes tagging double-stranded polynucleotides with duplex tags, sequencing polynucleotides from the sample and estimating total number of polynucleotides mapping to selected genetic loci. The estimate of total number of polynucleotides can involve estimating the number of double-stranded polynucleotides in the original sample for which no sequence reads are generated. This number can be generated using the number of polynucleotides for which reads for both complementary strands are detected and reads for which only one of the two complementary strands is detected.

Abstract: The present disclosure provides a technique for efficiently detecting a wider variety of RNA higher-order structures including non-Watson-Crick base pair-type higher order structures. The method for analyzing the RNA higher-order structure according to the present disclosure comprises the steps of providing a compound in which a target-binding moiety Sm and an RNA-modifying moiety Y are linked by a linker L; contacting the compound and one or a plurality of RNAs; determining a nucleotide sequence of the RNA after contacting with the compound; and determining a position and/or a region on the RNA that interacts with the target binding moiety of the compound, based on the nucleotide sequence.

Abstract: Embodiments of systems, methods, and compositions provided herein relate to on bead tagmentation and droplet indexing. Some embodiments include performing co-assays on partitioned beads, including nucleic acid sequencing, indexed PCR, preparing nucleic acid libraries, determining methylation status, identifying genomic variants, or protein analysis.

Abstract: The present disclosure provides methods, compositions, and devices for the rapid and direct detection of the sex of a fetus. The disclosure also provides methods, compositions, and devices for detecting fetal nucleic acids in biological samples (e.g., blood, cervical mucus, or urine).

Abstract: The present invention relates to methods, devices and kits for identifying individuals who are at increased risk of developing severe symptoms associated with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. The present invention also relates to methods, devices and kits for identifying individuals who have a reduced risk of developing severe symptoms associated with SARS-CoV-2 infection. In particular, it relates to the use of HLA haplotypes as biomarkers to predict disease severity in individuals infected with SARS-CoV-2.

Abstract: Provided herein are methods and compositions for the detection of silent carriers of chromosomal deletion alleles in a human subject using haploid cells (e.g., sperm cells or egg cells) derived from the subject. The methods provided herein allow for the detection of silent (2+0) carriers of SMA, where the individual has a deletion of the SMN1 gene on one chromosome 5 homolog and two or more copies of the SMN1 gene on the other chromosome 5 homolog.

Abstract: The present disclosure relates to a method for selecting mesenchymal stem cells having improved self-maintenance ability, wherein mesenchymal stem cells having excellent self-proliferative ability may be selected to reduce donor variation, and mesenchymal stem cells having excellent self-proliferative ability may be secured in large quantities and used for the development of cell therapy products.

Abstract: The present disclosure relates to materials and methods for spatially analyzing nucleic acids that have been fragmented with a transposase enzyme, alone or in combination with other types of analytes.

Abstract: The invention provides methods and compositions for hybridizing at least one molecule to a target. The invention may, for example, utilize a of cyclic and/or non-cyclic solvent that is non-toxic and may eliminate or reduce the amount of formamide in the hybridization composition.

Abstract: Disclosed herein are kits and methods for assessing the risk of poor reading performance in an individual by detecting and identifying single nucleotide polymorphisms in chromosome 19, e.g. in the KIAA0355 (GARRE1) gene. Also disclosed herein are risk alleles in chromosome 19 that are associated with a latent measure for reading ability.

Abstract: A method of analyzing the therapeutic efficacy of a therapeutic agent for treating an immune-related disorder is disclosed. The method comprises contacting blood cells of the subject with the agent, measuring the expression of at least 20 genes in the blood cells of the subject; obtaining an immune age value based on the expression of the at least 20 genes, and comparing the immune age value with an immune age value of the subject calculated on the basis of expression of the at least 20 genes in blood cells of the subject, in the absence of the agent.

Abstract: Provided are a marker for detecting the severity of atopic dermatitis and a method for detecting the severity of atopic dermatitis using the marker.

Abstract: A method for detecting or monitoring FSHD comprising detecting one or more biomarkers, such as miRNA biomarkers or protein biomarkers that are significantly decreased or increased in subjects having FSHD compared to normal control subjects. Methods for treatment of subjects at risk of having, or having FSHD.

Abstract: Biomarkers and methods for identifying, verifying and confirming circulating serum-based microRNAs. The microRNAs (PrognomiRs) can be used to differentiate patient's suffering from rapid progressing Parkinson's disease (PD) from slow progressing PD patients.

Abstract: The invention relates to means and methods for assessing clonal immunoglobulin (IG)/T cell receptor (TR) gene rearrangements in a clinical, diagnostic and/or research setting. Provided is a quality control composition comprising a mixture of genomic DNA isolated from a set of nine cultured cell lines, said set comprising the B cell lines ALL/MIK (ALL), Raji (Burkitt lymphoma), REH (B cell precursor ALL), TMM (CML-BC/EBV+B-LCL), TOM-1 (B cell precursor ALL), WSU-NHL (B cell lymphoma) and the T cell lines JB6 (ALCL), Karpas299 (ALCL) and MOLT-13 (ALL), or wherein one or more cell lines of said set is replaced with one or more other cell line(s) comprising the same IG/TR gene rearrangements. Also provided is a quality control composition consisting of essentially equimolar amounts of genomic DNA isolated from healthy human thymus, healthy human tonsil and healthy human peripheral blood mononuclear cells.

Abstract: The present disclosure provides a method for generating a calculated cancer signature for a cancer-related phenotype based on copy number alterations (CNAs) in a patient sample. The calculated cancer signature may correspond to a somatic mutation, an mRNA expression signature, or a protein expression signature. The disclosure also provides a method treating a patient using the calculated cancer phenotype. In addition, the disclosure provides a method for generating a calculated signature based on CNAs to replicate a cancer phenotype.

Abstract: The present invention includes a method for analyzing tRNA fragments. In one aspect, the present invention includes a method of identifying a subject in need of therapeutic intervention to treat a disease or condition, disease recurrence, or disease progression comprises characterizing the identity of tRNA fragments. The invention further includes diagnosing, identifying or monitoring a disease or condition, a panel of engineered oligonucleotides, a kit for a high-throughput assay, and a method and system for identifying tRNA fragments.

Abstract: Described herein are methods, devices, and systems for measuring a level, presence, recurrence, progression, or regression of a disease (such as cancer), for example a fraction of nucleic acid molecules (such as cell-free DNA) in a sample from an individual that relate to diseased tissue (such as cancer tissue). The methods include generating, using the sequencing data comprising sequencing reads associated with loci selected from a personalized disease-associated small nucleotide variant panel, a plurality of variant motif-specific models that each associate sequencing data corresponding to a respective variant motif, a background factor indicative of a false positive error rate for the respective variant motif, and an estimated fraction of the nucleic acid molecules associated with the disease. From the plurality of variant motif-specific models, a fraction of the nucleic acid molecules associated with the disease for the individual can be determined.

Abstract: There is described herein a method of prognosing and/or predicting disease progression and/or in subject with prostate cancer, the method comprising: a) providing a sample containing mitochondrial genetic material from prostate cancer cells; b) sequencing the mitochondrial genetic material with respect to at least 1 patient biomarker selected from CSB1, OHR, ATP8 and HV1 (hypervariable region 1); c) comparing the sequence of said patient biomarkers to control or reference biomarkers to determine mitochondrial single nucleotide variations (mtSNVs); and d) determining the a prostate cancer prognosis; wherein a relatively worse outcome is associated with the presence of mtSNVs in CSB1, OHR, ATP8 and a relatively better outcome is associated with the presence of mtSNVs in HV1.

Abstract: The present invention relates to a novel biomarker for predicting prognosis of treatment of HER2-positive breast cancer and the use thereof. The present invention presents the novel biomarker for predicting prognosis of treatment of HER2-positive breast cancer, and provides a composition and a kit for predicting prognosis of treatment of HER2-positive breast cancer, which can detect or measure the biomarker in HER2 early-positive breast cancer patients to whom neoadjuvant chemotherapy is applicable. Furthermore, according to the present invention, it is possible to select a patient-specific therapy by quickly and accurately determining the prognosis of drug treatment of the patient.

Abstract: Provided herein are compositions, systems, kits, and methods for performing an activity based on detecting, in a sample from a cancer patient, the presence of elevated levels of Lactotransferrin (LTF) mRNA or protein, or detecting the presence in the MIF promoter region of at least one of: ?173C and ?794 CATT5-8, and treating a patient with immunotherapy, or generating a report that the subject should be treated with immunotherapy.

Abstract: The present disclosure relates to a molecular marker of the IGF2BP1 gene related to a chicken body size trait and the use thereof, and a genetic improvement method. In the present disclosure, it is found by means of gene association analysis that a deletion mutation in a promoter region of the IGF2BP1 gene of chicken is significantly associated with the chicken body size trait.

Abstract: An objective of the present invention is to provide a system for predicting disease contraction and a method for predicting disease contraction for predicting whether there is a possibility that an animal will contract a specific disease. The system for predicting disease contraction is provided with: a reception means for receiving information on the presence/absence of bacteria that belong to Fusobacteriaceae and/or Veillonellaceae in a sample collected from an animal; and an assessment means for performing predictive assessment regarding the possibility that the animal will contract keratoconjunctivitis sicca from the information, input to the reception means, on the presence/absence of bacteria that belong to Fusobacteriaceae and/or Veillonellaceae.

Abstract: Methods of detecting the absence or presence of a micro-organism in a sample comprising: contacting the sample with a nucleic acid molecule which acts as a substrate for nucleic acid modifying activity of the micro-organism in the sample, incubating the thus contacted sample under conditions suitable for nucleic acid modifying activity; and specifically determining the absence or presence of a modified nucleic acid molecule resulting from the action of the nucleic acid modifying activity on the substrate nucleic acid molecule to indicate the absence or presence of the micro-organism. Corresponding kits are also provided.

Abstract: The present invention provides methods for identifying or selecting a Brassica plant cell, Brassica plant or part thereof, or a Brassica seed comprising a QTL that confers resistance against clubroot disease. The present invention further provides a nucleic acid comprising a QTL that confers resistance against clubroot disease as well as markers associated therewith, proteins encoded by the nucleic acid, plants or parts thereof, plant cells or seeds comprising the QTL and uses thereof.

Abstract: Systems comprising a translation module, an amplification module comprising one or more sensing modalities, and a detection module configured to accept the amplifier output and methods of using the same are provided. The systems or methods can be capable of detecting trace amounts of nucleic acid molecules with high sensitivity and specificity in a device-free system with naked-eye readable output. The systems can comprise three layers incorporated into a lateral flow assay and/or a paper strip assay, in such systems, the first layer can comprise the translation module, the second layer can comprise the amplification module, and the third layer can comprise the detection module.

Abstract: Provided herein are synthetic nucleic acid molecules known as loop-mediated riboregulators that have single-nucleotide polymorphism (SNP) sensitivity and ultralow OFF state signal levels. Loop-mediated riboregulators can activate or repress gene expression in response to trigger RNAs bearing completely arbitrary sequences. Also provided herein are methods of using such synthetic nucleic acid molecules for detecting the presence or absence of a particular target RNA in, for example, a biological sample.

Abstract: Provided herein are, inter alia, kits and methods for the detection SARS-CoV-2 in COVID-19 patients. The kits and methods provided herein are, interalia, useful for the rapid detection of SARS-CoV-2 and related variants in, for example, the saliva of COVID-19 patients.

Abstract: Embodiments of the present disclosure provide for embodiments of a panel of oligonucleotides for use in a multiplex reverse transcriptase-polymerase chain reaction (RT-PCR) assay for the identification of rotavirus A and B and genotypes thereof. Another aspect of the disclosure encompasses embodiments of an RT-PCR multiplex method for determining whether an equine is infected with an equine rotavirus.

Abstract: An immune molecule virus particle detection kit is provided. A monoclonal antibody of a virus envelope antigen is modified with biotin, magnetic beads are coupled with streptavidin, and the biotin-modified monoclonal antibody is incubated with a virus-containing solution to form a complex with virus particles or antigens, and then the streptavidin-coupled magnetic beads are added for incubation. The streptavidin on the magnetic beads binds with the biotin-modified monoclonal antibody with high specificity and affinity, and then specifically captures the virus particles with envelopes. After separating a supernatant through a magnetic separator, complete viral particles, empty-shell viruses, and free envelope antigens can be separated from other virus components, and the magnetic bead conjugates can be qualitatively or quantitatively detected through polymerase chain reaction (PCR) amplification.

Abstract: A crystallization apparatus for a crystallization of a preliminary product. The crystallization apparatus includes an inlet for receiving the preliminary product, a rotary drive, and a rotatable receiving unit having, on an upper side thereof, a circular surface and/or an annular surface for the crystallization so that, when the rotatable receiving unit is rotated, the preliminary product received can be recrystallized in a defined manner on the circular surface and/or the annular surface and can be discharged as a crystallized product.

Abstract: A method for producing a recycled leather fabric according to an embodiment of the present invention may comprise steps of: processing cowhide pieces or waste to produce cotton-like leather fiber; preparing a mixture by mixing the cotton-like leather fiber with cotton-like fiber; producing a leather thread by spinning the mixture; weaving the leather thread to produce a fabric; crushing and beating cowhide to produce cowhide short fibers; laminating the cowhide short fibers onto the fabric; and entangling the laminated cowhide short fibers with the leather thread by pressing the cowhide short fibers into the fabric.

Abstract: A method for operating a blast furnace for producing of pig iron, comprising the steps of including heating a stream of hydrocarbon gas and a stream of steam in a first heater to provide a heated stream of hydrocarbon gas and steam, feeding and partially reforming the heated stream of hydrocarbon gas and steam in a pre-reformer to provide a stream of partially reformed syngas, heating a first stream of blast furnace gas from the blast furnace and the stream of partially reformed syngas in a second heater, before or after their mixing together, to provide a heated carbon feed stream, reforming the heated carbon feed stream in a secondary reformer to provide a second stream of syngas, and feeding said second stream of syngas to the shaft of the blast furnace.

Abstract: A method of producing pig iron in a shaft furnace is provided. The shaft furnace is charged in an upper region with raw materials which fall within the shaft furnace under the influence of gravity. A portion of the raw materials is melted and/or partly reduced under the action of the atmosphere that exists within the shaft furnace. A hot gas stream which is introduced in a lower region of the shaft furnace flows through and influences the atmosphere that exists within the shaft furnace in terms of chemical composition and temperature. A cold gas stream is fed to a heat exchanger in which the cold gas stream is heated to a temperature higher than 700° C. to give a hot gas stream. The cold gas stream comprises a CO2 component of at least 5% by volume. The cold gas stream may contain, air and/or pure oxygen as residual component.

Abstract: The present disclosure provides a low-temperature-resistant and corrosion-resistant cargo oil tank steel suitable for high-heat-input welding and a manufacturing method therefor. The low-temperature-resistant and corrosion-resistant cargo oil tank steel suitable for high-heat-input welding includes, by weight in percent, 0.04%-0.13% C, 0.10%-0.40% Si, 0.60%-1.30% Mn, 0.005%-0.012% P, S?0.006%, 0.01%-0.05% Al, 0.03%-0.15% Sn, 0.005%-0.020% Nb, 0.005%-0.025% Ti, 0.15%-0.40% Ni, 0.15%-0.50% Cu, 0.10%-0.25% Cr, 0.007%-0.024% Ca and the balance Fe and inevitable impurities. The corrosion-resistant steel provided by the present disclosure is mainly designed for the upper deck and inner bottom plate of a storage and transportation tank of a polar route oil tanker, and the steel has excellent low-temperature toughness and can be welded with large heat input.

Abstract: A thin steel sheet having a chemical composition satisfying 0.0001?[Sn]+0.3[Cu]+0.1[Cr]?0.2000 ([Sn], [Cu], and [Cr] respectively being the contents (mass %) of Sn, Cu, and Cr), having an oxide film of a thickness of 50 nm or less on the surface, and satisfying A/C<0.30 and B/C<3.0 when measuring the oxide film by the FT-IR reflectance method (A is an area of a peak derived from SiO2, B is an area of a peak derived from MnSiO3, and C is an area of a peak derived from Mn2 SiO4).

Abstract: According to one aspect of the present invention, a hot-rolled steel sheet and a manufacturing method for same may be provided, wherein the hot-rolled steel sheet has excellent yield strength, vibration damping ratio, electrical resistivity, and low-temperature toughness, and thus has properties suitable for use in a hyper train tube.

Abstract: According to one aspect of the present invention, a hot-rolled steel sheet and a manufacturing method for same may be provided, wherein the hot-rolled steel sheet has excellent yield strength, vibration damping ratio, and low-temperature toughness, and thus has physical properties suitable for use in a hyper train tube.

Abstract: A cold-rolled steel sheet having excellent processability according to an exemplary embodiment of the present invention contains, by wt %: 0.012 to 0.060% of C, 0.03% or less (excluding 0%) of Si, 0.1 to 0.4% of Mn, 0.015 to 0.050% of Al, 0.015% or less (excluding 0%) of P, 0.015% or less (excluding 0%) of S, 0.006% or less (excluding 0%) of N, and a balance of Fe and other unavoidable impurities.

Abstract: One embodiment of the present invention provides: a high toughness high carbon cold rolled steel sheet having excellent formability comprising, by weight %, C: 0.80% to 1.25%, Mn: 0.2% to 0.6%, Si: 0.01% to 0.4%, P: 0.005% to 0.02%, S: 0.01% or less, Al: 0.01% to 0.1%, Cr: 0.01% to 1.0%, Sn: 0.05% to 0.5%, the remainder being Fe and other unavoidable impurities, in which a microstructure includes, by area %, retained austenite: 1% to 10%, martensite: 1% to 10%, ferrite: 5% or less (including 0%), the remainder being bainite, and has an average grain size of 3 ?m to 20 ?m, and an internal oxide layer formed directly below the surface has a thickness of 10 ?m or less; and a method for manufacturing same.

Abstract: A method for heating a powder material to induce a crystalline phase change in the grains of the particle comprising the steps of: a. preheating the powder from the high temperature streams generated from cooling the phase changed product; b. injecting the powder into a metal tube; c. controlling the gas composition in the metal tube by injecting a gas into the reactor; d. externally heating the first section of the tube by a first furnace segment system; e. externally heating the second section of the tube by a second furnace segment system; f: quickly quenching the powder product temperature in a cold third segment of the tube; g. collecting the processed powder at the base of the tube in a bed ejecting the powder from the tube; h. cooling the powder in a heat exchanger and using the heat to preheat the powder in step a.