Abstract: A release agent and method of use for preventing the adhesion of materials to equipment in various industries including construction, mining, and asphalt. The release agent can include oil, surfactant, and in increased level of free fatty acids that can combine with alkali and/or alkaline earth metals in the materials to form a metal/fatty acid salt that prevents adhesion and material build up.

Abstract: Method for preventing or reducing low speed pre-ignition events in a spark-ignited internal combustion engine is provided. The method includes supplying to the engine the lubricant composition comprising a primary additive having a structure given by or a salt thereof. R1 and R2 are independently H, C1-C20 hydrocarbyl group, carboxyl group, ester, amide, ketone, ether, or hydroxyl group. R3 and R4 are independently H, C1-C20 hydrocarbyl group, carboxyl group, ester, amide, ketone, ether, amino, or hydroxyl group or wherein R3 and R4 are part of a cyclic group. R5 is C1-C100 hydrocarbyl group, carboxyl group, ether, or hydroxyl group. Lastly, p is 0 to 2, n is 1 to 5, m is 0 to 2, and p+n+m is less than 6.

Abstract: This disclosure describes a zinc-free lubricating oil composition. The composition includes a major amount of an oil of lubricating viscosity having a kinematic viscosity at 100° C. in a range of about 1.5 to about 35 mm2/s and an anti-wear mixture having one or more ashless dispersants and an alkyl phosphonic acid having a structure given by wherein R is a C3-C20 hydrocarbyl group. The initial pH of the anti-wear mixture is between 5-9.5 as measured by ASTM D664.

Abstract: A lubricating oil composition may contain a base oil (A) and a dispersant-type viscosity index improver (B), in which the nitrogen atom content based on the solid content of the dispersant-type viscosity index improver (B) is 0.50 to 1.50% by mass, and the weight-average molecular weight (Mw) thereof is 100,000 or more, and the content in terms of the solid content based on the total amount of the composition of the dispersant-type viscosity index improver (B) is more than 0.05% by mass and less than 5.0% by mass. The lubricating oil composition may exert excellent wear resistance in a state contaminated with soot.

Abstract: Object A grease composition having a low dust generation, long life, and low low-temperature torque under an ultra high-speed, high-temperature environment with a rotational speed exceeding 30000 rpm is provided, and by applying the grease composition to a rolling bearing, the rolling bearing having a low dust generation, long life and low low-temperature torque, and a motor including such a rolling bearing are also provided. Resolution Means Provided are: a grease composition containing a base oil and a thickener, the base oil including a fluorine oil, an ester oil, and a polyalphaolefin oil, and the thickener including a fluorine-based thickener, a urea-based thickener, and a barium complex soap thickener, each of these components being included in the grease composition at a predetermined proportion; a rolling bearing filled with the grease composition; and a motor including the rolling bearing.

Abstract: The present invention relates to a process for enzymatic esterification of free fatty acids. In particular the invention relates to this process using a flash operation for water removal from enzyme reaction mixture.

Abstract: Disclosed herein are detergent compositions containing extended chain surfactants that form microemulsions with and can remove greasy and oily stains. In certain embodiments the extended nonionic surfactant includes Guerbet C10 or C12(PO)8(EO)n. The detergent compositions and methods of employing the same beneficially clean soils from textiles including difficult to remove cosmetic soils and food oils, even those comprised of non-trans fats.

Abstract: Cleaning systems employing detergent compositions in combination with builders comprising alkalinity and chelants are provided. The detergent compositions include amine oxide surfactant and alcohol ethoxylate surfactant blends having a mixture of chain lengths and branching provided. The detergent compositions are particularly efficacious for flexible cleaning systems for cosmetic and other soils as they provide the benefit of separate dosing of surfactants in the detergent from alkalinity sources in a builder composition. Methods of using the detergent compositions and/or the cleaning systems are provided.

Abstract: This disclosure relates to methods and compositions for treating a semiconductor substrate having a pattern disposed on a surface of the substrate.

Abstract: A liquid fabric treatment composition suitable for encapsulation in a unit dose pack contains from about 3% by weight to about 8% by weight of an alcohol ethoxylate, from about 10% by weight to about 70% by weight of an encapsulated fragrance slurry; from about 0.5% by weight to about 5% by weight of a rheology modifier; from about 0.1% by weight to about 3% by weight of a pH adjusting agent; and from about to about 5% by weight to about 60% by weight of non-aqueous solvent. The liquid composition is stable at elevated temperatures and has a lower yield stress and a lower storage modulus than a composition having no alcohol ethoxylate.

Abstract: The invention includes a solid rinse aid that is particularly designed for pressed or extrusion solid formation and which is effective for spotless surfaces after rinsing, especially rinsing metals without corrosion. According to the invention, a solid acid is combined with a short-chain alkylbenzene and alkyl naphthalene sulfonate. The short-chain alkylbenzene and alkyl naphthalene sulfonate act as a solidification agent as well as a hydrotrope, and total dissolved solid (TDS) active and are combined with at least one nonionic low foaming surfactant.

Abstract: Detergent compositions effective for controlling hard water scale accumulation are disclosed. Detergent compositions employing phosphinosuccinic acid and mono-, bis- and oligomeric phosphinosuccinic acid (PSO) derivatives with alkali metal carbonate and/or alkali metal hydroxide reduce hard water scale accumulation on treated surfaces at alkaline conditions between about pH of 9 and 12.5. Methods employing the detergent compositions and preventing hard water scale accumulation are also provided.

Abstract: A phosphate-free automatic dishwashing cleaning composition comprising i) a protease wherein the protease is a variant having at least 60% identity with the amino acid sequence of SEQ ID NO:1 and comprising at least one amino acid substitution (using the SEQ ID NO: 1 numbering) selected from the group consisting of X54T; X126A, D, G, V, E, K, I; X127E, S, T, A, P, G, C; and X128E, C, T, D, P, G, L, Y, N; and ii) from 10 to 50% by weight of the composition of a complexing agent system comprising from 0 to less than 30% by weight of the composition of citric acid.

Abstract: A hard surface cleaning composition, methods of cleaning a hard surface with a low hysteresis composition and disposable premoistened pads for cleaning hard surfaces are provided. The hard surface cleaning composition includes from 0.01 wt. % to 7 wt. % of a solvent selected from the group consisting of a propylene glycol phenyl ether, a dipropylene glycol phenyl ethers and mixtures thereof and at least 92% water. The cleaning composition has a pH from about 3.5 to 9.5 and is free of anionic surfactant and alkanolamine.

Abstract: A hard surface cleaning composition, methods of cleaning a hard surface with a low hysteresis composition and disposable premoistened pads for cleaning hard surfaces are provided. The hard surface cleaning composition includes from 0.01 wt. % to 7 wt. % of a solvent selected from the group consisting of a propylene glycol phenyl ether, a dipropylene glycol phenyl ethers and mixtures thereof and at least 92% water. The cleaning composition has a pH from about 3.5 to 9.5 and is free of anionic surfactant and alkanolamine.

Abstract: An aqueous cleaning liquid including hydrofluoric acid and tetradecylphosphonic acid, and a method of cleaning an electronic device, contacting the electronic device with the aqueous cleaning liquid.

Abstract: Embodiments of the present disclosure herein include a method of removing a contamination material from an optical device. The method may include disposing an optical device in a process chamber, the optical device having optical device structures formed in a substrate, the contamination material is disposed at least on sidewalls of the optical device structures and within trenches between the optical device structures, and exposing the optical device to a plasma generated in the process chamber, the plasma generated from oxygen gas (O2), chlorine gas (Cl2), Argon (Ar), or a combination thereof, the exposing the optical device to the plasma removes the contamination material.

Abstract: The present invention provides packaged beverage containing an edible aqueous solution and a hydrophobic droplet containing a hydrophobic aroma component, wherein the aqueous solution and the hydrophobic droplet are separated, and also provides a method for producing a packaged beverage in which an edible aqueous solution and a hydrophobic droplet containing a hydrophobic aroma component are separated, the method including: packing the aqueous solution in a container main body and subsequently adding a hydrophobic liquid composition containing a hydrophobic aroma component, or packing the hydrophobic liquid composition in the container main body and subsequently adding the aqueous solution, and then sealing the container main body.

Abstract: An alcoholic beverage that contains one or more types of steviol glycosides selected from rebaudioside D and rebaudioside M, and has a steviol glycoside content of 0.04-0.5 g/1,000 ml and an alcohol content of 3.0 to 40.0 v/v %.

Abstract: A rapid method of brewing malt beverages having 0.0% alcohol by volume with the use of yeast in aerobic conditions. The yeast is inactivated by boiling after undergoing fermentative growth, during either aerobic or anaerobic conditions. Aspects of the beverage such as sweetness, aldehyde content, flavor-positive components, pH, mouthfeel and heat are controlled to emulate popular styles of beer. The beverage may also be fortified with alcohol to create alcoholic products, such as beer, kombucha, hard seltzer, wine spirits in a rapid time frame.

Abstract: The present disclosure provides a bioreactor that utilizes cultivated algae grown within a sealed chamber to convert carbon dioxide in fluid that is passed through the sealed chamber into oxygen via photosynthesis. The apparatus comprises an LED array for providing artificial light to the algae, which causes them to utilize CO2 in the surrounding fluid for photosynthesis, producing oxygen as a result. Transparent inserts are disposed within the sealed chamber for increasing the amount of surface area on which the algae can form and increasing efficiency.

Abstract: The present invention relates to a culture device and culture method for culturing a 3D cell aggregation and, specifically, to an overall technique for effectively culturing a 3D cell aggregation by using a culture device including a porous microwell, a membrane, and other additive components.

Abstract: A neural lattice device includes a substrate having formed therein one or more wells and one or more supply ducts. A channel network includes one or more channels configured to establish fluid communication among the at least one well and the at least one supply duct. A reservoir is coupled to the substrate and configured to hold a fluid, and a cover is disposed against an upper surface of the substrate and configured to hermetically seal the wells, the supply ducts and the channel network.

Abstract: Described herein are systems and methods for generating a product enriched in one or more target particles, comprising a cassette to which the one or more microfluidic cartridges are releasably coupled and supported, the cassette comprising (i) a plurality input containers releasably and fluidically coupled thereto, wherein at least one of the plurality of input containers comprises an incoming sample, (ii) a plurality of outlets having a plurality of output containers releasably and fluidically coupled thereto, (iii) one or more microfluidic cartridges for separating one or more target particles from the sample, and (iv) a plurality of fluidic channels extending between the plurality of inlets, the plurality of outlets, and the one or more microfluidic cartridges, wherein the cassette having the plurality of input containers, the plurality of output containers, and the one or more microfluidic cartridges coupled thereto, collectively provides a closed end-to-end sterile environment that enables inline contin

Abstract: Systems and devices for producing biological products and methods of using the same are provided. An example universal skid includes a rigid support and a plurality of flow-channel hardware sets. Each flow-channel hardware set couples with a respective flow channel of a plurality of flow channels for unit operations. The flow-channel hardware sets allow the flow channels to operate in parallel or in series for continuous processing over the unit operations. An example modular assembly includes one or more single-use kits for one or more unit operations and a universal skid coupled with the one or more single-use kits. An example manufacturing system includes multiple modular assemblies coupled with each other allowing continuous processing within each modular assembly, in between the modular assemblies, fully continuous processing across multiple unit operations.

Abstract: Cells may be grown in a bioreactor, and the cells may be activated by an activator (e.g., a soluble activator complex). Nutrient and gas exchange capabilities of a closed, automated cell expansion system may allow cells to be seeded at reduced cell seeding densities, for example. Parameters of the cell growth environment may be manipulated to load the cells into a particular position in the bioreactor for the efficient exchange of nutrients and gases. System parameters may be adjusted to shear any cell colonies that may form during the expansion phase. Metabolic concentrations may be controlled to improve cell growth and viability. Cell residence in the bioreactor may be controlled. In embodiments, the cells may include T cells. The cells may include T cell subpopulations, including regulatory T cells (Tregs), for example.

Abstract: An apparatus for culturing suspended photosynthetic organisms, specifically a flowing thin film photobioreactor, wherein the flowing thin film photobioreactor comprises a light source and a trickle-film insert comprising screen material.

Abstract: A 3D bioprinter having a visible light source to photocure biomaterial is disclosed. The 3D bioprinter prints visible light-curable biomaterial along with viable cells, and visible light photocures the biomaterial while maintaining cell viability. Visible light 3D bioprinter systems and methods of printing are further disclosed.

Abstract: A method and apparatus for preventing condensation on a cell culture plate transferred from an incubator to an imager. A block having a higher thermal conductivity layer and a lower thermal conductivity layer is preheated in an incubator. The block is placed on a cell culture plate either before or immediately after removing the cell culture plate from an incubator with the higher thermal conductivity layer resting on the plate and configured to overlie all of the wells of the plate. The culture plate is transported to an imager with the thermal block in place and the block is removed before inserting the culture plate into the imager.

Abstract: The present disclosure is directed to a bioreactor or fermenter for the culturing of cells or microorganisms in suspension in a liquid medium in industrial scale comprising a vessel containing the culture in a liquid medium having a determined filling height; a stirrer provided in the vessel to stir the liquid medium; a first sparger arranged in the bottom portion of the vessel; and a second or optional more spargers provided above the first sparger to supply additional air bubbles and/or additional oxygen gas bubbles continuously to the liquid medium whereby the second sparger is located at a position in the bioreactor or fermenter above the first sparger in a predefined distance ?. It is also described a process for the independent management of dissolved CO2 and O2, by selecting a suitable modified gas flow rate qmod.

Abstract: Disclosed herein are devices and methods for processing biologic tissues, such as adipose tissue or whole blood, via centrifugation. The devices comprise a rotatable chamber having a first outlet, a tube connected or connectable to the first outlet, a collection container in fluid communication with the tube; and a drive unit configured to rotate the rotatable chamber about the axis. The device is operable such that a sample of biologic tissue present in the rotatable chamber can be stratified into at least two constituent layers as a function of the differing specific gravities of the constituents, at least one of the constituent layers being dischargeable from the rotatable chamber via the tube and into the collection container.

Abstract: Novel methods and apparatus are disclosed for cell culture and cell recovery. The methods and apparatus simplify the process of cell separation from media, minimize potential damage to gas permeable devices during fluid handling, and allow closed system automated cell culture and cell recovery from gas permeable devices.

Abstract: The present application relates to a novel strain of the genus Schizochytrium (Schizochytrium sp.) and a method of producing polyunsaturated fatty acids by using the same. According to one aspect, novel microalgae of the genus Schizochytrium have a high content of fat in biomass, and particularly, a high content of polyunsaturated fatty acids such as docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA), and therefore, the microalgae, and biomass or bio-oil prepared therefrom, may be useful as a feed source or the like.

Abstract: The present invention relates to a Dunaliella salina OH214 strain having an improved ability to produce pigments, and in particular lutein. As it is possible to produce carotenoid pigments, specifically xanthophylls, with the Dunaliella salina OH214 strain by consuming less energy, it is possible to efficiently produce pigments at an industrial level. In addition, the Dunaliella salina OH214 strain can be applied as a raw material for foods, health functional foods, and pharmaceuticals, containing pigments. Moreover, the Dunaliella salina OH214 strain is a domestic native microalga in Korea and thus does not cause any GMO issues, it is possible to reduce costs by using seawater as a culture medium in Korea, and the effect of related industrial development can be expected.

Abstract: The objective of the present invention is to provide a method and a system for continuous production of microorganisms without the need for sterilization. The present invention provides a novel method for continuously producing microorganisms by proliferating a stored microorganism inoculum without the need for sterilization and a system therefor. According to the present invention, a novel method and system for producing microorganisms in which the need for sterilization for avoiding contamination with unwanted bacteria is reduced as compared with general methods and systems for producing microorganisms are provided.

Abstract: The present disclosure discloses a novel strain of Glutamicibacter, derived from insects, which efficiently degrades bifenthrin, belonging to the field of microbial strains. The Glutamicibacter CCTCC NO: M20221445 of the present disclosure was isolated from the intestinal tract of bifenthrin-resistant Ectropis grisescens Warren larvae. It exhibits unique genomic characteristics, growth and phenotypic traits, physiological and biochemical characteristics, as well as the ability to utilize and degrade bifenthrin efficiently. Specifically, it can effectively degrade bifenthrin. Based on phenotypic features, physiological and biochemical characteristics, chemical composition, and molecular biology-based polyphasic classification, Glutamicibacter CCTCC NO: M20221445 is identified as a new species. This bacterium possesses the capability to efficiently degrade bifenthrin, laying the foundation for biological control of E. grisecens and offering new microbial resources to address pesticide residue problems.

Abstract: Disclosed herein include methods, compositions and culture media for generating synthetic embryos in vitro from mammalian pluripotent stem cells such as pluripotent embryonic stem cells. In some embodiments, the method can comprise co-culturing a wild-type mammalian pluripotent stem cell and modified mammalian pluripotent stem cells comprising one or more genes encoding transcription factors that can drive generation of extraembryonic cells or extraembryonic-like cells (e.g., GATA6 gene, SOX17 gene, GATA3 gene and/or TFAP2C gene) under a condition in a culture medium allowing the pluripotent stem cells to self-organize into a post-implantation embryo structure. In some embodiments, the pluripotent embryonic stem cells are human pluripotent embryonic stem cells and the generated synthetic embryo is a human embryo.

Abstract: The present invention concerns a novel human erythroid progenitor cell line, wherein at least 90% of the cells are CD36+ CD44?CD71+; and wherein the cells:—do not express the gene encoding the receptor of Granulocyte-macrophage colony-stimulating factor (GM-CSF-R gene) or express GM-CSF-R gene at a lower level than the cells of human UT-7/Epo-S1 cell line; and—express the gene encoding the receptor of erythropoietin (Epo-R gene). The present invention also concerns the uses thereof for producing, detecting, or quantifying parvovims B19. The present invention allows the use of the cell lines for 1) a highly sensitive B19 infectious particles detection, and, 2) the efficient production of infectious B19 particles.

Abstract: The use of IL-2R??c agonist peptides in cell manufacturing is disclosed. The IL-2R??c agonist peptides can facilitate the selective growth of immune cell populations. Culture media comprising the IL-2R??c agonist peptides, methods of expanding a target immune cell population, and methods of manufacturing a target immune cell population are disclosed. The enriched immune cell populations can be used in immunotherapy.

Abstract: Methods of expanding a natural killer (NK) cell fraction for transplantation into a subject are provided, and particularly, methods for providing transplantable NK cell fractions and protocols for their use, which can be employed for applications in cell transplants and infusions for treatment of cancer and other disease.

Abstract: The invention relates to multipotential expanded mesenchymal precursor progeny (MEMP's), characterised by the early developmental markers STRO-1bri and ALP. The present invention also relates to methods for producing MEMP's and to uses of MEMP's for therapeutic applications.

Abstract: The invention features pancreatic islet and pancreatic organoids, and cell cultures and methods that are useful for the rapid and reliable generation of pancreatic islet and pancreatic islet organoids. The invention also features methods of treating pancreatic diseases and methods of identifying agents that are useful for treatment of pancreatic diseases, such as type 2 diabetes and pancreatic cancer, using the pancreatic islet and pancreatic organoids of the invention.

Abstract: Disclosed herein are methods, compositions, kits, and agents useful for inducing ? cell maturation, and isolated populations of SC-? cells for use in various applications, such as cell therapy.

Abstract: Provided are a HEK293T cell strain having high dispersibility and a screening method therefor. Specifically, the present application relates to a method for screening a HEK293T cell strain suitable for serum-free suspension culture, a cell strain screened by using the method, and a method for producing a viral vector by using the cell strain.

Abstract: The present disclosure relates generally to ex vivo primary tumor models prepared from fresh tumor tissues which are useful for screening anti-cancer agents. The fresh tumor tissues are prepared and cultured under suitable conditions to grow an outgrowth of endothelial cells Killing of these endothelial cells by a candidate agent indicates the efficacy of the agent in inhibiting tumor angiogenesis.

Abstract: A closed, automated and scalable stirred tank bioreactor platform, capable of sustaining high fold expansion of hPSCs is provided. hPSCs are expanded in a controlled bioreactor using perfused xeno-free media. Cell harvest and concentration are performed in closed steps. The hPSCs can be cryopreserved to generate a bank of cells or further processed as needed. Cryopreserved cells can be thawed into a 2D tissue culture platform or a 3D bioreactor to initiate a new expansion phase or be differentiated to the clinically relevant cell type. The expanded hPSCs express hPSC-specific markers, have a normal karyotype and the ability to differentiate to the cells of the three germ layers. This end-to-end platform allows large expansion of high quality hPSCs that can support the required cell demand for various clinical indications.

Abstract: Described herein are compositions and methods for generating a viable cell that expresses at least one or more woolly mammoth genes. Also described herein are compositions and methods for generating an embryo, blastula, oocyte, or non-human organism that expresses one or more woolly mammoth genes.

Abstract: The invention relates to a method for obtaining spheroids of differentiated cells, in particular of adipocytes without an exogenous matrix. These spheroids are particularly suitable for carrying out metabolic studies on test compounds by mimicking as well as possible the functioning of an organ in vivo such as adipose tissue, for example.

Abstract: Described herein are compositions and kits comprising recombinant adeno-associated viruses (rAAVs) with tropisms showing increased specificity and efficiency of viral transduction in targeted cell types such as the brain and lung. The rAAV compositions described herein also have tropisms showing decreased specificity and decreased efficiency of viral transduction in an off-target cell type such as the liver. The rAAV compositions described herein encapsidate a transgene, such as a therapeutic nucleic acid. Upon systemic delivery to a subject, the rAAV is capable of increased specificity and increased transduction of the transgene in a target cell-type, as compared to a parental or reference AAV.

Abstract: Provided herein are methods that include introducing into an inner ear of a mammal a therapeutically effective amount of an adeno-associated virus (AAV) vector that includes a nucleotide sequence encoding (a) a polypeptide including an antibody heavy chain variable domain operably linked to a signal peptide and a polypeptide including an antibody light chain variable domain operably linked to a signal peptide; (b) a polypeptide including an antigen-binding antibody fragment operably linked to a signal peptide; or (c) a soluble vascular endothelial growth factor receptor operably linked to a signal peptide.