Patents Issued in March 28, 2024
  • METHODS AND COMPOSITIONS FOR TREATING, PREVENTING OR REVERSING OBESITY AND OBESITY-RELATED DISORDERS BY OPSIN 3 REGULATION OF HYPOTHALAMIC MELANOCORTIN RECEPTORS
    Publication number: 20240102021
    Abstract: Described herein are methods and compositions for the prevention or treatment of obesity and obesity-related disorders. The methods and compositions are based, inter alia, on the observations that OPN3 is the most highly expressed opsin in the hypothalamus, a key site for the regulation of energy homeostasis. Indeed, OPN3 expression was highest in regions associated with energy homeostasis, namely the paraventricular nucleus and arcuate nucleus of the hypothalamus. OPN3 was shown to interact and form a complex with MC3R and MC4R, and to modulate MC3R- and MC4R-mediated signaling in the hypothalamus. Accordingly, the methods involve the regulation of melanocortin receptors by downregulating opsin 3 (OPN3) protein expression, OPN3 gene expression, and/or OPN3 activation in the hypothalamus.
    Type: Application
    Filed: April 28, 2023
    Publication date: March 28, 2024
    Inventor: Elena OANCEA
  • MODULATING BONE MORPHOGENIC PROTEIN (BMP) SIGNALING IN THE TREATMENT OF ALZHEIMER'S DISEASE
    Publication number: 20240102022
    Abstract: Methods and compositions are provided for the treatment of Alzheimer's Disease (AD) by administering to a patient a therapeutically effective amount of an agent that inhibits signaling mediated by a bone morphogenetic protein type 1A receptor (BMPR-1A) or bone morphogenetic protein type 2 receptor (BMPR-2). Also provided are methods and compositions to increase the rate of neural stem cell self-renewal.
    Type: Application
    Filed: November 17, 2023
    Publication date: March 28, 2024
    Applicants: CZ Biohub SF, LLC, The Board of Trustees of the Leland Stanford Junior University
    Inventors: Elizabeth Yang Chen, Felicia Reinitz, Jane Antony, Michael F. Clarke, Robert C. Jones
  • NOVEL RNA APTAMER INTERVENES ESTROGEN RECEPTOR INTERACTION WITH COACTIVATOR MED1 TO OVERCOME BREAST CANCER METASTASIS
    Publication number: 20240102023
    Abstract: The present disclosure concerns RNA aptamers that specifically inhibit the protein-protein interaction between MED1 and an estrogen receptor. As shown herein, the aptamers are specific for the LXXLL recognition motif between MED 1 and an estrogen receptor. The present disclosure further concerns pRNA nanoparticles of the RNA aptamers coupled with a further HER2 aptamer that show HER2 specific uptake and subsequent inhibition of cellular proliferation and metastasis in vitro and in vivo. Furthermore, the pRNA nanoparticles showed no adverse effects, signifying a safe and effective composition to specifically target and control HER2 expressing cells.
    Type: Application
    Filed: October 14, 2020
    Publication date: March 28, 2024
    Applicant: University of Cincinnati
    Inventor: Xiaoting Zhang
  • RECOMBINANT BACTERIA ENGINEERED TO TREAT DISEASES ASSOCIATED WITH METHIONINE METABOLISM AND METHODS OF USE THEREOF
    Publication number: 20240102024
    Abstract: The present disclosure provides host cells, e.g., bacterial cells, that comprise a methionine decarboxylase enzyme for the treatment of diseases and disorders associated with methionine metabolism in a subject. The disclosure further provides pharmaceutical compositions and methods of treating disorders associated with methionine metabolism, such as homocystinuria.
    Type: Application
    Filed: November 30, 2023
    Publication date: March 28, 2024
    Inventors: Dylan Alexander Carlin, Vincent M. Isabella, Jonathan McMurry, Theodore Carlton Moore, III, Mylene Perreault, Nathan Schmidt, Mark Simon
  • GENE COMBINATION FOR EXPRESSING AND PRODUCING TERREQUINONE A IN ESCHERICHIA COLI AND USE THEREOF
    Publication number: 20240102025
    Abstract: The present disclosure provides a gene combination for expressing and producing terrequinone A in Escherichia coli and use thereof. The gene combination includes a tdiAS gene, a tdiBS gene, a tdiCS gene, a tdiDS gene, a tdiES gene, an sfpS gene, an ScCKS gene, and an AtIPKS gene with nucleotide sequences set forth in SEQ ID NOS:1 to 8. In the present disclosure, a recombinant engineered strain capable of producing terrequinone A having anti-cancer activity is obtained by separately constructing recombinant plasmids pC02 and pU03 through the eight genes and transforming the two recombinant plasmids into E. coli. The content of terrequinone A in a fermentation broth thereof is 106.3 mg/L, which has potential application value in the biopharmaceutical field.
    Type: Application
    Filed: May 24, 2023
    Publication date: March 28, 2024
    Inventors: Yongsheng TIAN, Lijuan WANG, Yongdong DENG, Quanhong YAO, Rihe PENG, Jianjie GAO, Zhenjun LI, Wenhui ZHANG, Bo WANG, Jing XU, Yu WANG, Xiaoyan FU, Hongjuan HAN
  • MICROBIOME MODULATION OF A HOST BY DELIVERY OF DNA PAYLOADS WITH MINIMIZED SPREAD
    Publication number: 20240102026
    Abstract: The present invention concerns nucleic acids of interest for modulating the microbiome of a host, to vectors encoding said nucleic acids and to methods for in vivo modulating the microbiome of a subject by delivering said nucleic acid of interest.
    Type: Application
    Filed: July 7, 2023
    Publication date: March 28, 2024
    Inventors: Jesus FERNANDEZ RODRIGUEZ, Xavier DUPORTET
  • Composition and Method of an Orally Administered Antimicrobial Peptide Vectored in a Bacterial Expression Vehicle
    Publication number: 20240102027
    Abstract: The inventive subject matter includes an antimicrobial peptide vectored composition made of a bacterial protein expression vehicle expressing one or more recombinant antimicrobial peptide effector molecules. More specifically these novel recombinant antimicrobial peptide effector molecules exhibit preferential antimicrobial activity. In some embodiments, the antimicrobial activity is facilitated via interaction with the lipid bilayer of the parasitic cell form, leading to membrane pore formation and resulting in cell lysis. The utility of the current inventive subject matter has demonstrated a reduced viability of Eimeira acervulina sporozoites was shown in vitro using a sporozoite killing assay.
    Type: Application
    Filed: October 4, 2023
    Publication date: March 28, 2024
    Applicants: US BIOLOGIC, INC, The United States of America as represented by the Secretary of Agriculture
    Inventors: Jolieke G. Van Oosterwijk, Luciana Meirelles Richer, Douglas Steven Zetechka, JR., Hyun Soon Lillehoj, Woohyun Kim, Cyril Gay, Christopher Anthony Przybyszewski
  • METHODS AND COMPOSITIONS FOR EFFICIENT GENETIC MODIFICATIONS OF BACILLUS LICHENIFORMIS STRAINS
    Publication number: 20240102028
    Abstract: The instant disclosure is generally related to compositions and methods for obtaining and constructing Bacillus licheniformis host cells (e.g., protein production host cells, cell factories) having increased protein production capabilities. Certain embodiments of the disclosure are directed to efficient genetic modifications of B. licheniformis cells and the subsequent selection of such B. licheniformis cells having increased protein production capabilities. Certain other embodiments of the disclosure are generally related to methods and compositions for producing/obtaining auxotrophic B. licheniformis cells, wherein certain other embodiments of the disclosure are directed to methods and compositions for restoring prototrophy in auxotrophic B. licheniformis cells, and expressing genes of interest (GOIs) in such restored prototrophy B. licheniformis cells.
    Type: Application
    Filed: November 30, 2023
    Publication date: March 28, 2024
    Inventors: Dennis De Lange, Marc Anton Bernhard Kolkman, Frank Wouter Koopman, Chris Leeflang
  • TWO-PLASMID SYSTEM FOR PRIME EDITING IN YEAST, USE THEREOF, AND METHOD FOR PRIME EDITING IN YEAST
    Publication number: 20240102029
    Abstract: The present disclosure relates to a two-plasmid system for prime editing in yeast, use thereof, and a method for gene prime editing in yeast. The two-plasmid system includes a first plasmid and a second plasmid. The first plasmid includes a sequence encoding for an epegRNA. The epegRNA is an RNA molecule including a motif at a 3?-terminus of a pegRNA, and the motif having a sequence as set forth in SEQ ID NO. 12. The second plasmid includes a sequence encoding for a fusion protein of a nucleic acid nickase nCas9 fused with a reverse transcriptase M-MLV RT. The two-plasmid expression system can be used in gene editing in yeast.
    Type: Application
    Filed: April 16, 2023
    Publication date: March 28, 2024
    Inventors: Zehua BAO, Xiaoxiao SHI, Weiyu XIE, Lei DENG, Zhenkun CAI
  • Inducible Production-Phase Promoters for Coordinated Heterologous Expression in Yeast
    Publication number: 20240102030
    Abstract: Inducible promoters for the coordinated expression of at least one heterologous gene in yeast and methods of using them are disclosed. In particular, the invention relates to sets of inducible promoters derived from S. cerevisiae and related species that can be induced in the presence of nonfermentable carbon sources.
    Type: Application
    Filed: September 6, 2023
    Publication date: March 28, 2024
    Applicant: The Board of Trustees of the Leland Stanford Junior University
    Inventors: Colin Harvey, Ulrich Schlecht, Maureen Elizabeth Hillenmeyer
  • OPTIMIZED HOST/VECTOR SYSTEM FOR PRODUCING PROTECTIVE MONO- AND MULTIVALENT SUBUNIT VACCINES ON THE BASIS OF THE YEAST KLUYVEROMYCES LACTIS
    Publication number: 20240102031
    Abstract: Described herein are recombinant Kluyveromyces lactis (K. lactis) yeasts which are capable of the highly efficient expression of one or more foreign proteins and are suitable for use as a vaccine for generating a protective immune response against pathogens. The invention provides in particular K. lactis strains for the targeted cloning of foreign antigen-coding nucleic acids into the yeast genome of the K. lactis strain, which is characterized in that the K. lactis strain has integrated expression cassettes for foreign antigens as an alternative or in addition to the KILAC4 locus on the KIURA3-20 locus (KLLA0E22771g) and/or on the KIMET5-1 locus (KLLA0B03938g). The invention further relates to integrative expression vectors and to methods for producing the K. lactis strains of the invention as well as to the use thereof as vaccines.
    Type: Application
    Filed: October 5, 2023
    Publication date: March 28, 2024
    Applicant: VEROVACCINES GMBH
    Inventors: Hans Caspar HÜHRLIMANN, Martina BEHRENS, Mandy GEBAUER, Karin BREUNIG, Sven-Erik BEHRENS
  • CRISPR-CAS EFFECTOR POLYPEPTIDES AND METHODS OF USE THEREOF
    Publication number: 20240102032
    Abstract: The present disclosure provides CRISPR-Cas effector polypeptides that exhibit enhanced gene editing and/or trans cleavage activity, compared to a wild-type CasPhi polypeptide. The present disclosure provides systems and kits comprising such CRISPR-Cas effector polypeptides. The present disclosure provides methods, including gene editing and diagnostic methods, using a CRISPR-Cas effector polypeptide of the present disclosure.
    Type: Application
    Filed: January 24, 2022
    Publication date: March 28, 2024
    Inventors: Jennifer A. Doudna, Patrick Pausch, Katarzyna Soczek, Steven E. Jacobsen, Zheng Li
  • ISOLATION AND CHARACTERIZATION OF NOVEL TISSUE-SPECIFIC PROMOTERS IN MAIZE
    Publication number: 20240102033
    Abstract: The present invention provides constructs comprising a transgene operable linked to a novel tissue-specific maize promoter. Methods of using the constructs to drive tissue-specific transgene expression in a maize plant and maize plants comprising the constructs are also provided.
    Type: Application
    Filed: September 22, 2023
    Publication date: March 28, 2024
    Inventors: Heidi Kaeppler, Nathaniel Schleif
  • CANNABIS PLANT WITH INCREASED CANNABIGEROLIC ACID
    Publication number: 20240102034
    Abstract: The present technology generally relates to Cannabis plants having increased cannabigerolic acid (CBGA) and/or cannabigerol (CBG) content. The present technology also generally relates to isolated nucleic acids and isolated amino acids related to same and methods of producing same.
    Type: Application
    Filed: July 15, 2021
    Publication date: March 28, 2024
    Inventor: Daniel Kevin FOWLER
  • OLEIC ACID -ENRICHED PLANT BODY HAVING GENETICALLY MODIFIED FAD2 AND PRODUCTION METHOD THEREOF
    Publication number: 20240102035
    Abstract: The present invention relates to an artificially manipulated unsaturated fatty acid biosynthesis-associated factor and use thereof to increase the content of a specific unsaturated fatty acid of a plant body. More particularly, the present invention relates to a system capable of artificially controlling unsaturated fatty acid biosynthesis and a plant body produced thereby, which include an artificially manipulated unsaturated fatty acid biosynthesis-associated factor to control unsaturated fatty acid biosynthesis and a composition capable of artificially manipulating the factor. In a specific aspect, the present invention relates to artificially manipulated unsaturated fatty acid biosynthesis-associated factors such as FAD2, FAD3, FAD6, FAD7 and FAD8 and/or an unsaturated fatty acid biosynthesis controlling system by an expression product thereof.
    Type: Application
    Filed: November 29, 2023
    Publication date: March 28, 2024
    Inventors: Seok Joong KIM, Ok Jae KOO, Min Hee JUNG, Ye Seul KIM
  • POLYACTIVE CARBOHYDRATES AND METHODS OF USE THEREOF
    Publication number: 20240102036
    Abstract: Described herein are biological devices and methods for using the same to produce a polyactive carbohydrate. The biological devices include microbial cells transformed with a DNA construct containing genes for producing a chitin synthase, a chitosanase, 5 and a chitin deacetylase, and, optionally, lipase, regulatory sequence CHR1, transglycosylase, dehydrogenase, and/or (1?3), (1?4)-?-glucan synthase. Methods for using the polyactive carbohydrate are also provided herein including, but not limited to, increasing plant hormone production and improving the appearance and root strength of plants and production of polyurethane biofoams.
    Type: Application
    Filed: October 6, 2020
    Publication date: March 28, 2024
    Inventor: Raul CUERO RENGIFO
  • TRANSGENIC PLANTS WITH ENHANCED TRAITS
    Publication number: 20240102037
    Abstract: This disclosure provides transgenic plants having enhanced traits such as increased yield, enhanced nitrogen use efficiency and enhanced drought tolerance; propagules, progeny and field crops of such transgenic plants; and methods of making and using such transgenic plants. This disclosure also provides methods of producing hybrid seed from such transgenic plants, growing such seed and selecting progeny plants with enhanced traits. Also disclosed are transgenic plants with altered phenotypes which are useful for screening and selecting transgenic events for the desired enhanced trait.
    Type: Application
    Filed: July 26, 2023
    Publication date: March 28, 2024
    Inventors: Paolo Castiglioni, Jaishree M. Chittoor-Vijayanath, Barry S. Goldman, Robert J. Meister, Monnanda S. Rajani, G. Ramamohan, Naveen Silvester, Tyamagondlu V. Venkatesh, Jingrui Wu, Xiaoyun Wu
  • COMPOSITIONS AND METHODS FOR PRODUCING TOBACCO PLANTS AND PRODUCTS HAVING REDUCED OR ELIMINATED SUCKERS
    Publication number: 20240102038
    Abstract: The present disclosure provides the identification of genes involved in sucker growth in tobacco. Also provided are promoters that are preferentially active in tobacco axillary buds. Also provided are modified tobacco plants comprising reduced or no sucker growth. Also provided are methods and compositions for producing modified tobacco plants comprising reduced or no sucker growth.
    Type: Application
    Filed: December 12, 2023
    Publication date: March 28, 2024
    Applicant: ALTRIA CLIENT SERVICES LLC
    Inventors: Dongmei XU, Jesse FREDERICK, Chengalrayan KUDITHIPUDI, Yanxin SHEN, James STRICKLAND, Jaemo YANG
  • MODULATING LIGHT RESPONSE PATHWAYS IN PLANTS, INCREASING LIGHT-RELATED TOLERANCES IN PLANTS, AND INCREASING BIOMASS IN PLANTS
    Publication number: 20240102039
    Abstract: Methods and materials for modulating low light and/or shade tolerance, and red light specific responses in plants are disclosed. For example, nucleic acids encoding low light and/or SD+EODFR-tolerance polypeptides are disclosed as well as methods for using such nucleic acids to transform plant cells. Also disclosed are plants having increased low light and/or SD+EODFR tolerance. In addition, methods and materials involved in increasing UV-B tolerance in plants and methods and materials involved in modulating biomass levels in plants are provided.
    Type: Application
    Filed: September 20, 2023
    Publication date: March 28, 2024
    Inventors: Shing Kwok, Kenneth Bounds, Ryan Miller, Sam Harris, James Burns, Roger I. Pennell, Vijay Sharma, Michael F. Portereiko, Han-Suk Kim, Gerard Magpantay
  • NUCLEOTIDE SEQUENCES AND CORRESPONDING POLYPEPTIDES CONFERRING MODULATED GROWTH RATE AND BIOMASS IN PLANTS GROWN IN SALINE CONDITIONS
    Publication number: 20240102040
    Abstract: The present invention relates to isolated nucleic acid molecules and their corresponding encoded polypeptides able confer the trait of improved plant size, vegetative growth, growth rate, seedling vigor and/or biomass in plants challenged with saline conditions. The present invention further relates to the use of these nucleic acid molecules and polypeptides in making transgenic plants, plant cells, plant materials or seeds of a plant having plant size, vegetative growth, growth rate, seedling vigor and/or biomass that are improved in saline conditions with respect to wild-type plants grown under similar conditions.
    Type: Application
    Filed: October 9, 2023
    Publication date: March 28, 2024
    Inventors: Fasong Zhou, Kenneth A. Feldmann, Julissa Sosa
  • NUCLEOTIDE SEQUENCES AND CORRESPONDING POLYPEPTIDES CONFERRING MODULATED GROWTH RATE AND BIOMASS IN PLANTS GROWN IN SALINE CONDITIONS
    Publication number: 20240102041
    Abstract: The present invention relates to isolated nucleic acid molecules and their corresponding encoded polypeptides able confer the trait of improved plant size, vegetative growth, growth rate, seedling vigor and/or biomass in plants challenged with saline conditions. The present invention further relates to the use of these nucleic acid molecules and polypeptides in making transgenic plants, plant cells, plant materials or seeds of a plant having plant size, vegetative growth, growth rate, seedling vigor and/or biomass that are improved in saline conditions with respect to wild-type plants grown under similar conditions.
    Type: Application
    Filed: October 26, 2023
    Publication date: March 28, 2024
    Inventors: Fasong Zhou, Kenneth A. Feldmann, Julissa Sosa
  • METHODS AND COMPOSITIONS FOR SELECTIVE REGULATION OF PROTEIN EXPRESSION
    Publication number: 20240102042
    Abstract: The invention provides novel recombinant DNA molecules, compositions, and methods for selectively regulating the expression of a transcribable polynucleotide molecule or recombinant protein in a male reproductive tissue of a transgenic plant. The invention also provides transgenic plants, plant cells, plant parts, seeds, and commodity products comprising such DNA molecules and compositions.
    Type: Application
    Filed: November 8, 2023
    Publication date: March 28, 2024
    Inventors: Jintai Huang, Youlin Qi, Heping Yang, Yuanji Zhang
  • POLYNUCLEOTIDES AND POLYPEPTIDES OF PLANT AND BACTERIAL ORIGIN FOR PROTECTING PLANTS FROM PATHOGENIC FUNGI
    Publication number: 20240102043
    Abstract: The present invention relates to polynucleotides and polypeptides derived from plants and bacteria which are associated with increased resistance of plants to pathogenic fungi and/or Oomycetes. The fungicidal polypeptides of the invention are particularly effective in combating fungi inducing root rot and stalk rot in plants. The present invention further provides methods of using the polynucleotides and polypeptides of the invention for controlling plant fungal/Oomycetes pathogens and for producing transgenic plants having increased resistance to the pathogens.
    Type: Application
    Filed: July 27, 2023
    Publication date: March 28, 2024
    Applicant: Evogene Ltd.
    Inventors: Tidhar Turgeman, Ada Viterbo-Fainzilber, Eyal Emmanuel
  • T CELL RECEPTORS THAT BIND TO NY-ESO-1 AND METHODS OF USE THEREOF
    Publication number: 20240102044
    Abstract: Provided are TCRs (e.g., TCRs that bind to NY-ESO-1), cells and pharmaceutical compositions comprising these TCRs, nucleic acids encoding these TCRs, expression vectors and host cells for making these TCRs, and methods of treating a subject using these TCRs.
    Type: Application
    Filed: March 27, 2023
    Publication date: March 28, 2024
    Applicant: MiNK Therapeutics, Inc.
    Inventors: Marc van Dijk, Volker Seibert, Cornelia Anne Mundt, Arthur Andrew Hurwitz, Mark Adrian Exley, Benjamin Jacob Wolf, Daniel Leventhal, Sébastien Lalevée, Reed Masakayan
  • VECTORS, GENETICALLY MODIFIED CELLS, AND GENETICALLY MODIFIED NON-HUMAN ANIMALS COMPRISING THE SAME
    Publication number: 20240102045
    Abstract: Provided herein are genetically modified cells and genetically modified non-human animals (e.g., rats and mice) comprising: (i) a Rag1 and/or Rag2 gene knock-out; (ii) a IL2rg gene knock-out; and (iii) a homozygous null mutation in the non-human animal Heme oxygenase-1 (Hmox-1) gene, and optionally a Fah gene knock-out and/or expressing one or more human or humanized polypeptides. Methods and compositions of making and using such genetically modified cells and non-human animals are also provided.
    Type: Application
    Filed: July 18, 2023
    Publication date: March 28, 2024
    Inventors: Davor Frleta, Naxin Tu
  • METHODS FOR ANALYSIS OF VIRAL CAPSID PROTEIN COMPOSITION
    Publication number: 20240102046
    Abstract: Methods of determining the stoichiometry of a viral capsid and/or determining the heterogeneity of protein components in a viral capsid are disclosed.
    Type: Application
    Filed: May 17, 2022
    Publication date: March 28, 2024
    Inventor: Shunhai Wang
  • An oncolytic virus vector coding for variant interleukin-2 (vIL-2) polypeptide
    Publication number: 20240102047
    Abstract: The present invention provides an oncolytic adenoviral vector comprising a nucleic acid sequence encoding a variant interleukin 2 (vIL-2) polypeptide as a transgene. The present invention also provides a pharmaceutical composition comprising said oncolytic vector and at least one of the following: physiologically acceptable carriers, buffers, excipients, adjuvants, additives, antiseptics, preservatives, filling, stabilising and/or thickening agents. A particular aim of the present invention is to provide said oncolytic viral vector or pharmaceutical composition for use in the treatment of cancer or tumor, preferably a solid tumor.
    Type: Application
    Filed: October 12, 2020
    Publication date: March 28, 2024
    Inventors: Sadia Zafar, Dafne Quixabeira, Riikka Havunen, Akseli Hemminki
  • GENE THERAPY FOR RETINAL DISEASES
    Publication number: 20240102048
    Abstract: A viral vector comprises a transgene construct. In some embodiments, the transgene construct is a DNA sequence comprising a promoter element, a retinal gene's open reading frame being longer than 10 kb, and a terminator sequence. In other embodiments, the transgene construct is a DNA segment of retinal genes longer than 10 kb.
    Type: Application
    Filed: February 14, 2022
    Publication date: March 28, 2024
    Inventor: Uwe D. STAERZ
  • Helper-dependent adenoviral gene therapy delivery and expression system
    Publication number: 20240102049
    Abstract: The present invention relates to gene therapy delivery and expression systems comprising at least one helper-dependent adenoviral vector containing a nucleic acid sequence encoding for proteoglycan 4 (PRG4) or a biologically active fragment thereof. The invention further relates to a pharmaceutical composition comprising a therapeutically effective amount of at least one helper-dependent adenoviral vector containing said nucleic acid sequence encoding for proteoglycan 4 (PRG4), or a homolog thereof from any other species, or a biologically active fragment thereof. The invention also relates to the use of the novel gene therapy delivery and expression system according to the invention for use in the prevention and/or treatment of camptodactyly-arthropathy-coxa vara-pericarditis (CACP), or a musculoskeletal disorder such as a joint disorder or Joint disease.
    Type: Application
    Filed: July 14, 2023
    Publication date: March 28, 2024
    Inventors: Merry RUAN, Kilian GUSE, Brendan LEE
  • COMPOSITIONS AND METHODS FOR TREATMENT OF NEUROLOGICAL DISORDERS
    Publication number: 20240102050
    Abstract: The present disclosure provides methods and compositions for the treatment of diseases and/or disorders in a subject, including, but not limited to neurological disorders such as giant axonal neuropathy. The methods described herein include direct administration of a gene therapy (e.g. an rAAV viral vector) to a subject via injection into a vagus nerve (e.g. the left vagus nerve) of the subject.
    Type: Application
    Filed: July 19, 2023
    Publication date: March 28, 2024
    Inventors: Rachel M. BAILEY, Steven J GRAY
  • MULTIFUNCTIONAL COPOLYMERIC NANOPARTICLE
    Publication number: 20240102051
    Abstract: A multifunctional copolymeric nanoparticle, including a plurality of 1,4-diazabicyclo[2.2.2]octane (DABCO) monomers crosslinked with a plurality of bromoacetyl cystamine (BBAC) monomers. Also, each of the plurality of DABCO monomers and each of the plurality of BBAC monomers alternate in sequence. the multifunctional copolymeric nanoparticle has a formula: wherein n is an integer between 5 and 40.
    Type: Application
    Filed: November 20, 2023
    Publication date: March 28, 2024
    Applicants: Iran University of Medical Sciences
    Inventors: Seyyed Emad Hooshmand, Mahdi Karimi, Amir Hossein Vahabi
  • PNMA2-BASED CAPSIDS AND USES THEREOF
    Publication number: 20240102052
    Abstract: Disclosed herein, in certain embodiments, are recombinant PNMA2 and endogenous Gag polypeptides, capsids comprising the recombinant PNMA2 or endogenous Gag polypeptides, and methods of making and using recombinant PNMA2 and endogenous Gag polypeptides.
    Type: Application
    Filed: January 26, 2022
    Publication date: March 28, 2024
    Inventors: Colin MALONE, Oleg LAPTENKO, Adam FRAITES, Jessica CRISP, Andrey PISAREV, Anil ANGAD, Heley ONG, Zachary GILBERT, Jason D. SHEPHERD
  • Method for Producing Genetically Engineered Cells
    Publication number: 20240102053
    Abstract: Disclosed is a method for producing genetically engineered cells in vitro. The method includes direct injection of a genome editing composition into a cell nucleus of a cell. The genome editing composition includes at least one Cas protein and at least one gRNA molecule to target a distinct genomic location. The injection is performed with a microelectromechanical systems injection chip including a cantilever. The cantilever includes a microchannel being in fluid communication with a nanosyringe, and wherein direct injection includes providing a fluid communication between the microchannel and the nucleus of the cell by insertion of the nanosyringe into the nucleus of the cell and injecting the genome editing composition via the microchannel through the nanosyringe into the nucleus of the cell.
    Type: Application
    Filed: February 4, 2022
    Publication date: March 28, 2024
    Inventors: Tobias A. Beyer, Michael Gabi, Stephan Jud, Matyas Vegh, Maria Mila, Samuel Riedmann, Paul Monnier, Pascal Behr
  • METHOD OF REDUCING NITROGEN OXIDE CONCENTRATION IN SAMPLE, BIOREACTOR, AND PLUG FLOW REACTOR
    Publication number: 20240102054
    Abstract: A method of reducing a concentration of a nitrogen oxide, the method comprising: contacting a microorganism with a nitrogen oxide-containing sample to reduce the concentration of the nitrogen oxide in the sample, wherein the contacting comprises contacting the microorganism with Fe(II)(L)-NOx in a bioreactor, wherein the Fe(II)(L)-NOx is a complex in which a chelating agent, Fe2+, and NOx are chelated, wherein L is the chelating agent, and wherein NOx is a nitrogen oxide ligand.
    Type: Application
    Filed: September 21, 2023
    Publication date: March 28, 2024
    Inventors: Seung Hoon Song, Heejoo Han, Woo Yong Shim, Sukhwan Yoon, Jae-Young Kim, Sojung Yoon, Yu Kyung Jung
  • METHOD FOR PRODUCING A FERMENTATION PRODUCT
    Publication number: 20240102055
    Abstract: The present invention relates to a method for producing a fermentation product, which method comprises (i) reducing carbon dioxide to a C1 compound, (ii) contacting at least a portion of said C1 compound with a culture comprising a methylotrophic microorganism, (iii) fermenting said C1 compound with said methylotrophic microorganism to produce said fermentation product, wherein the fermentation of the C1 compound with said methylotrophic microorganism further produces carbon dioxide, which is at least partially recycled to the reducing step.
    Type: Application
    Filed: December 8, 2021
    Publication date: March 28, 2024
    Inventors: Brecht Marcel VANLERBERGHE, Frederik Benoit Alphonsine DE BRUYN, Lieve Monique Cornelia HOFLACK, Johan Hilaire Corneel GHEERAERT
  • PROCESS FOR PRODUCING ETHANOL
    Publication number: 20240102056
    Abstract: The present invention relates to processes for producing fermentation products from starch-containing material, wherein a thermostable alpha-amylase and optionally a thermostable protease are present and/or added during liquefaction. The invention also relates to a composition suitable for use in a process of the invention.
    Type: Application
    Filed: October 23, 2023
    Publication date: March 28, 2024
    Applicant: NOVOZYMES A/S
    Inventors: Randy Deinhammer, Suzanne Clark, Mauricio Quiros, John Matthews, Anne Glud Hjulmand, Chee-Leong Soong, Tomoko Matsui, Shinobu Takagi
  • METHODS AND COMPOSITIONS USEFUL FOR THE PRODUCTION OF 4-VINYLPHENOL
    Publication number: 20240102057
    Abstract: The present invention provides for a method for producing a 4-vinylphenol (4VP) and/or 4-vinylguaiacol (4VG), the method comprising: (a) providing a host cell capable of expressing a polypeptide having a phenolic acid decarboxylase (PAD) enzymatic activity wherein the polypeptide is capable of converting p-coumaric (CA) and/or ferulic acid (FA) into 4-vinylphenol (4VP) and/or 4-vinylguaiacol (4VG), respectively; and (b) culturing the host cell in a culture medium to express the polypeptide such that the polypeptide converts CA and/or FA into 4VP and/or 4VG, respectively; wherein the culture medium comprises an organic overlay or phase.
    Type: Application
    Filed: September 13, 2023
    Publication date: March 28, 2024
    Inventors: Alberto RODRIGUEZ, Jamie A. MEADOWS, Ning SUN, Blake A. SIMMONS, John M. GLADDEN
  • Caproate-producing bacterium with multiple substrate utilization capabilities and its applications
    Publication number: 20240102058
    Abstract: The disclosure discloses Oscillospiraceae sp. LBM10036 capable of utilizing multiple substrates and application thereof, and belongs to the fields of microbial technology and Baijiu brewing. The microorganism Oscillospiraceae sp. LBM10036 of the disclosure was preserved in the Guangdong Microbial Culture Collection Center on Sept. 21, 2020, with the preservation number GDMCC No. 61202. The Oscillospiraceae sp. LBM10036 of the disclosure has the capability to utilize diverse substrates and high product specificity, adapts to low pH, and can efficiently synthesize caproic acid under a pH of 5.0-5.5. The strain used in the disclosure can be used for strengthening microorganisms in pit mud, and improving the quality of degraded pit mud, to improve the quality of Strong aroma-type Baijiu, and also can be used for producing caproic acid from a variety of sugars and lactic acid by microbial fermentation.
    Type: Application
    Filed: September 27, 2023
    Publication date: March 28, 2024
    Inventors: Yan XU, Cong REN, Huilin WANG
  • METHOD FOR MANUFACTURING TRIACYLGLYCEROLS ENRICHED EITHER IN PALMITIC ACID AT SN-2 POSITION OR OLEIC ACID AT SN-2 POSITION
    Publication number: 20240102059
    Abstract: The present invention concerns an enzymatic process for the preparation of an ingredient comprising triacylglycerols enriched either in palmitic acid at sn-2 position or in oleic acid at sn-2 position.
    Type: Application
    Filed: February 3, 2022
    Publication date: March 28, 2024
    Inventor: AMAURY PATIN
  • ENZYMES AND METHODS FOR FERMENTATIVE PRODUCTION OF MONOTERPENE ESTERS
    Publication number: 20240102060
    Abstract: The present invention relates to an alcohol acyl transferase which is capable of esterifying a tertiary monoterpene alcohol such that at least 30% by mass of said tertiary monoterpene alcohol is esterified, preferably within 36 h, 24 h, 18 h, 12 h, 6 h, 3 h, 2 h, 1 h, 45 min or 30 min, more preferably in a microbial cell. The invention further relates to a nucleic acid comprising a nucleic acid sequence encoding the alcohol acyl transferase of the invention, or a complementary sequence thereof, and a vector or gene construct comprising the nucleic acid of the invention. Further provided by the present invention is a host cell comprising the vector or gene construct of the invention, and a transgenic non-human organism comprising the nucleic acid of the invention, the vector or gene construct of the invention, or the host cell of the invention.
    Type: Application
    Filed: December 17, 2021
    Publication date: March 28, 2024
    Inventors: Martinus Julius BEEKWILDER, Matthew Quinn STYLES, Aurin Minnert VOS, Thamara HESSELINK, Hendrik Jan BOSCH
  • METHODS OF IMPROVING PRODUCTION OF MORPHINAN ALKALOIDS AND DERIVATIVES
    Publication number: 20240102061
    Abstract: Methods and engineered cells are provided for increasing activity of a norcoclaurine synthase in a microbial cell. The method comprises, within the engineered microbial cell, contacting an engineered norcoclaurine synthase with a substrate, wherein contacting the substrate with the engineered norcoclaurine synthase increases conversion, within the engineered microbial cell, in comparison to a non-engineered norcoclaurine synthase.
    Type: Application
    Filed: November 18, 2021
    Publication date: March 28, 2024
    Inventors: Christina D. SMOLKE, Catherine THODLEY, KRISTY M. HAWKINS, Kenneth TAKEOKA, Douglas HANSEN
  • METHOD FOR PRODUCING TAGATOSE BY IMMOBILIZED MULTI-ENZYME SYSTEM
    Publication number: 20240102062
    Abstract: Provided are a method for preparing an immobilized multi-enzyme system, and a method for producing tagatose by the immobilized multi-enzyme system. The immobilized multi-enzyme system is formed by uniformly mixing a porous dopamine microsphere with a multi-enzyme mixture which is used for producing tagatose. Five enzymes in an enzymatic catalysis path for converting starch to tagatose are co-immobilized by means of a porous microsphere to obtain an immobilized multi-enzyme system, the immobilized multi-enzyme system is used to catalyze conversion of starch into tagatose, and thus, enzymes can be recycled, thereby greatly reducing the amount of enzymes required for preparation of tagatose, and reducing the production cost.
    Type: Application
    Filed: February 11, 2022
    Publication date: March 28, 2024
    Inventors: Yanhe MA, Ting SHI, Pingping HAN
  • NEW MAJOR FACILITATOR SUPERFAMILY (MFS) PROTEIN (FRED) IN PRODUCTION OF SIALYLATED HMOS
    Publication number: 20240102063
    Abstract: The present invention relates to the field of recombinant production of biological molecules in genetically modified cells. More particularly, it relates to a method for recombinant production of sialylated human milk oligosaccharides (HMO) using a genetically modified cell expressing a protein of the major facilitator superfamily (MFS), the protein expressed being Fred.
    Type: Application
    Filed: January 21, 2022
    Publication date: March 28, 2024
    Inventors: Margit PEDERSEN, Isotta D'ARRIGO, Katrine BYCH KAMPMANN, Manos PAPADAKIS
  • METHODS OF PROCESSING LIGNOCELLULOSIC BIOMASS USING SINGLE-STAGE AUTOHYDROLYSIS AND ENZYMATIC HYDROLYSIS WITH C5 BYPASS AND POST-HYDROLYSIS
    Publication number: 20240102064
    Abstract: The invention relates, in general, to methods of processing Lignocellulosic biomass to fermentable sugars and to methods that rely on hydrothermal pretreatment. Xylose monomer yields comparable to those achieved using two-stage pretreatments can be achieved from soft Lignocellulosic biomass feedstocks by pretreasting to very low severity in a single-stage pressurized hydrothermal pretreatment, followed by enzymatic hydrolysis to release xylose retained in the solid state. In some embodiments, pretreated biomass is separated into a solid fraction and a liquid fraction, the solid fraction subjected to enzymatic hydrolysis, and the separated liquid fraction subsequently mixed with the hydrolysed solid fraction.
    Type: Application
    Filed: November 29, 2023
    Publication date: March 28, 2024
    Applicant: Inbicon A/S
    Inventors: Jan Larsen, Niels Nielsen Poulsen, Martin Dan Jeppesen, Kit Kellebjerg Mogensen
  • METHOD OF REDUCING THE IMMUNOSTIMULATORY PROPERTIES OF IN VITRO TRANSCRIBED RNA
    Publication number: 20240102065
    Abstract: The present invention provides a method of reducing the immunostimulatory properties of an in vitro transcribed RNA by producing the in vitro transcribed RNA comprising a 3? terminal A nucleotide. Hereby, the circular DNA template used to generate the in vitro transcribed RNA has been linearized using a type IIS endonuclease. The invention further provides pharmaceutical compositions comprising the vitro transcribed RNA comprising a 3? terminal A nucleotide according to the invention for use in therapy.
    Type: Application
    Filed: January 26, 2022
    Publication date: March 28, 2024
    Applicant: CureVac SE
    Inventors: Moritz THRAN, Andreas THESS, Fabian EBER, Dipankar BHANDARI
  • RNA POLYMERASE VARIANTS
    Publication number: 20240102066
    Abstract: The present disclosure provides, in some aspects, variant RNA polymerases, the use of which increases transcription efficiency while reducing the number of double-stranded RNA contaminates and run-on transcripts produced during an in vitro transcription reaction.
    Type: Application
    Filed: August 11, 2023
    Publication date: March 28, 2024
    Applicant: ModernaTX, Inc.
    Inventors: Amy E. Rabideau, Athanasios Dousis, Kanchana Ravichandran, Elissa Hobert
  • Resynthesis Kits and Methods
    Publication number: 20240102067
    Abstract: This disclosure relates to novel mjresynthesis kits and methods, in particular for use in pairwise sequencing.
    Type: Application
    Filed: September 25, 2023
    Publication date: March 28, 2024
    Applicant: Illumina, Inc.
    Inventors: Kay Klausing, Jonathan Boutell, Trina Osothprarop, Oliver Miller, Justin Robbins
  • C-GLYCOSYLTRANSFERASE VARIANTS AND USE THEREOF
    Publication number: 20240102068
    Abstract: The present invention relates to novel C-glycosyltransferase variants and a use thereof. The C-glycosyltransferase variants according to the present invention have improved glycosidic bond-forming ability as compared with wild-type C-glycosyltransferase, and thus can increase the glycoside production effects of polyketide groups and pseudo-natural products, particularly type I, II, III polyketide, nonribosomal peptides, phenylpropanoids, and other aromatic natural products, and thus can be useful for the preparation of a drug, a food additive, a nutritional supplement, and the like containing a C-glycoside compound as a constituent ingredient.
    Type: Application
    Filed: January 27, 2022
    Publication date: March 28, 2024
    Inventors: Sang Yup LEE, Dongsoo YANG, Woo Dae JANG
  • METHODS AND COMPOSITIONS
    Publication number: 20240102069
    Abstract: The present invention relates to a biosynthetic route to intermediates of the QS-21 molecule which are triterpenes from a saponin extract of the tree Quillaja saponaria comprising a quillaic acid backbone, as well as routes to make the QS-21 molecule, enzymes involved, the products produced and uses of the product.
    Type: Application
    Filed: December 22, 2021
    Publication date: March 28, 2024
    Inventors: Anne OSBOURN, James REED, Anastasia ORME
  • FUNGAL STRAINS COMPRISING ENHANCED PROTEIN PRODUCTIVITY PHENOTYPES AND METHODS THEREOF
    Publication number: 20240102070
    Abstract: The present disclosure is generally related to modified filamentous fungal strains (cells) comprising enhanced protein productivity phenotypes, wherein such modified strains are particularly well-suited for growth in submerged cultures (e.g., large-scale production of proteins for industrial/commercial applications). Thus, certain embodiments of the disclosure are related to such variant (modified) strains of filamentous fungus derived (obtained) from parental strains comprising a gene encoding a native GEF1 protein, wherein the variant strain comprises a genetic modification which disrupts or deletes a gene encoding a native GEF1 protein, wherein the variant strain comprises an increased protein productivity phenotype (i.e., relative to the parental strain) when grown/cultivated/fermented under the same conditions.
    Type: Application
    Filed: November 6, 2020
    Publication date: March 28, 2024
    Inventors: Elizabeth A. Bodie, Zhongqiang Chen, Chuanbin Liu, Michael Ward