Abstract: An example method includes: recording, at a first sensor device, (i) a first informational signal as detected at the first sensor device; and (ii) a reference signal as detected at the first sensor device; recording, at a second sensor device (i) a second informational signal as detected at the second sensor device; and (ii) the reference signal as detected at the second sensor device; determining a time shift between the reference signal as recorded by the first sensor device and the reference signal as recorded by the second sensor device; and applying the determined time shift to the second informational signal to time-align the first informational signal and the second informational signal.

Abstract: The present invention relates to a device for detecting and/or analyzing an object or a material, wherein the device comprises a receiver configured to detect a first signal, the first signal being generated, at least in part, by vibrations of at least a part of the material or the object, and the device configured to communicate with a remote component, and to send a signal based, at least in part, on the first signal to the remote component. The present invention also relates to a remote component configured to communicate with a device, wherein the remote component is configured to at least receive data from the device and, based thereon, to determine data related to a material or an object, a system comprising the device and the remote component, a corresponding method, and a use of the system and method to detect and/or analyze the object or the material of a container.

Abstract: In one aspect, an automated, parallel autosampler system for drawing chromatography samples from a plurality of sample containers is provided which includes: a deck; a head movable relative to the deck; at least one drive selectively controllable to move the head relative to the deck; a plurality of elongated sippers mounted to the head at spaced-apart locations; a plurality of outlet tubes connected to the sippers in one-to-one correspondence; and, at least one source of negative pressure in selective communication with the outlet tubes to selectively provide negative pressure in the lumens of the sippers, wherein, the sippers are arranged on the head so that the sippers are simultaneously insertable into a corresponding number of the sample containers. Advantageously, the subject invention allows for much greater size samples to be extracted than the prior art with permitted variability in the number and configuration of the sample containers.

Abstract: A gas chromatograph includes a sample gas generator, a separation column configured to separate components of an introduced sample gas, a gas supply source configured to supply a carrier gas to the sample gas generator to carry the sample gas from the sample gas generator to the separation column when the components of the sample gas are analyzed, a controller configured or programmed to acquire an in-analysis usage amount of the carrier gas based on an analysis time for analyzing the components of the sample gas, and a flow rate of the carrier gas supplied from the gas supply source to the sample gas generator, and a display configured to display the in-analysis usage amount acquired by the controller.

Abstract: A liquid auto fraction apparatus for column chromatography includes a microtube mounting portion in which a plurality of microtubes for accommodating liquids to be fractionated according to column chromatography are mounted, a liquid supply unit configured to sequentially supplying the liquids to the plurality of microtubes, a light source unit provided on a rear side of the plurality of microtubes to emit light, an image acquisition unit provided on a front side of the plurality of microtubes to acquire images of the plurality of microtubes at preset time intervals while column chromatography is performed, a volume determination unit configured to extract heights of water surfaces of the accommodated liquids by analyzing the images of the plurality of microtubes acquired by the image acquisition unit, and a control unit configured to set a reference value for determining whether the volumes reach the target volume.

Abstract: A method for screening pesticide residues in medicine food homology (MFH) characteristic agricultural products includes step 1, a liquid chromatography-mass spectrometry database of pesticides is constructed, standard solution are prepared for the pesticides and liquid chromatography and mass spectrometry are used to detect the standard solutions; step 2, a sample is processed and then is detected to obtain a liquid chromatography-mass spectrogram; step 3, the liquid chromatography-mass spectrogram obtained from step 2 and liquid chromatography-mass spectrograms in the liquid chromatography-mass spectrometry database obtained from step 1 are compared and analyzed individually.

Abstract: The present technology relates to a method and instrument for classifying a sample. The method collects raw data from an analytical instrument (e.g., LC-MS), quantifies consistency parameters from the raw chromatographic data (e.g., peak detection) by assigning criteria and determining probability ratios, and constructs a learning model quantitation step by weighing the presence, absence, or modulation of one or more factors of the one or more samples against a decision criterion for positivity. The method can then apply the learning model to an unknown sample to detect the presence, absence, or modulation of one or more factors in the unknown sample such as the presence, absence, or modulation of a disease state or multiple disease states.

Abstract: The present invention provides a method for measuring the concentration and/or amount of one or more polysaccharide in a test sample comprising or consisting of the steps of (a) acid hydrolysis of the test sample with hydrochloric acid and trifluoroaceticacid; (b) chromatographic separation of the hydrolysed test sample of step (a); and (c) determining the concentration and/or amount of the one or more polysaccharide based on the data generated in step (b), together with processed test samples and kit for use of the same.

Abstract: A calibration concentration selection method includes steps of: using a gas sensor array to obtain a concentration variation sequence and a response variation sequence of the gas mixture; constructing and training an AE-BP model; constructing VAE and identically distributing the response variation sequence; inputting the identically distributed response variation sequence into the trained AE-BP model to output a predicted concentration variation sequence; and then normalizing the predicted concentration variation sequence to generate a target concentration variation sequence; sorting a target concentration variation sequence and calculating a response gradient sequence; processing the response gradient sequence for obtaining a corresponding smoothed gradient sequence; if the spike is greater than a preset hyperparameter, finding a large gradient concentration interval; and selecting concentration test points by random uniform sampling according to weights; and selecting concentration test points from all othe

Abstract: Gas detection devices comprise a dehydration unit a concentration unit, and a temperature control unit for controlling a temperature. The gas detection device includes a sampling mode in which sample gas flows into and out of the dehydration unit through a first port and a second port, respectively, wherein the volatile organic compounds in the sample gas are concentrated in the concentration unit. The temperature control unit may be configured such that the temperature of the sample gas after flowing out from the dehydration unit and before flowing into the concentration unit is not greater than a first preset temperature. Generation of condensed substances in the sample gas can be effectively avoided in a simple manner after the sample gas flows into the concentration unit, thereby further preventing ice blockage. Methods for detecting volatile organic compounds in a sample gas are also disclosed.

Abstract: A system, device, and method for augmenting environmental data is disclosed. The method includes (i) obtaining an environmental dataset having a first data resolution, and (ii) determining an augmented environmental dataset based at least in part on the environmental dataset, a set of spatial features, a set of temporal features, and a set of spatiotemporal features. The model has a second data resolution that is finer than the first data resolution.

Abstract: A method for displaying gas concentration values on a graphical display of a leak detector comprises detecting a presence of a gas using a gas sensor. A signal is generated by the gas sensor and transmitted from the gas sensor to a processor. The received signal is processed to determine a gas concentration value and a corresponding time stamp. The gas concentration values and corresponding time stamps are displayed graphically as they are determined and newly determined gas concentration values and corresponding time stamps are displayed in relation to previously determined gas concentration values and time stamps in streaming manner.

Abstract: A system and method for simultaneously running a plurality of air quality sensor units using a centralized station and software to test for field performance upon factory return from field installations, automatically identify preventative maintenance requirements, and automatically calibrate the sensor units and generate quality metric reporting. The centralized station and software include a computer, a manifold, tubes, and cables. A test gas is supplied, wherein the manifold distributes the test gas. Once powered and supplied with test gas, the sensor units are tested simultaneously. The computer runs software that compares the test data collected from the sensor units to determine if any of the sensor units fail standards or produce data that deviates from a mean average range. Sensor units that do not require maintenance are calibrated using test gasses to generate calibration coefficients which are stored within the sensors' firmware.

Abstract: A kit and powder composition are described that can be used to determine a concentration of orthophosphate in a water system. The kit includes a first powder composition including molybdate; and a second powder composition including ascorbic acid. A powder composition is also described that includes molybdate, pyrosulfate, and less than wt. % ascorbic acid. A method for determining the concentration of orthophosphate in a water system using the kit includes adding the first and second powder compositions to a water sample collected from the water system, measuring an absorbance of the water sample after the first and second powder compositions have been added to the water sample, and determining the concentration of orthophosphate in the water system based on the measured absorbance.

Abstract: An electrically insulated block-on-rotating-ring test unit and method, to evaluate the effects of electrical potential on material pairs and fluids under mechanical load and in sliding contact. The fluids may comprise lubricants and oils utilized in electrical vehicles.

Abstract: The present disclosure provides systems and methods for sorting a cell. The system may comprise a flow channel configured to transport a cell through the channel. The system may comprise an imaging device configured to capture an image of the cell from a plurality of different angles as the cell is transported through the flow channel. The system may comprise a processor configured to analyze the image using a deep learning algorithm to enable sorting of the cell.

Abstract: A coating and its application methods to coat at least one surface of a substrate, in particular cellulose-based substrates, and give it barrier properties to water, grease, oxygen, lights, moisture; heat resistance, a lower friction coefficient. The coating does not change the original properties of flexibility, biodegradability and recyclability of the original substrate. The coated substrate can be printed, can be recycled and exhibits unchanged properties in areas requiring folding of paper or cardboard.

Abstract: The present invention relates to methods for cancer detection using nematodes or nematodes GPCR receptors.

Abstract: A method and system for remotely monitoring intoxication of a user, comprising: prompting the user to provide a breath sample at a time point; at a breath sample acquisition device, generating a breath sample signal upon reception of the breath sample from the user, and broadcasting a unique signature proximal in time to the time point; using a sensor of a mobile computing device, generating an authentication signal derived from detection of the unique signature; at a processing system in communication with the mobile computing device and the sample acquisition device, receiving the breath sample signal and the authentication signal; generating a verification assessment that validates provision of the breath sample by the user; determining a value of an intoxication metric for the user based upon the breath sample signal; and transforming the verification assessment and the value of the intoxication metric into an analysis of intoxication of the user.

Abstract: Improved biomanufacturing devices, apparatuses and methods for using the same for monitoring biologic products such as biologies, vaccines, cell and gene therapies for viral safety and identification of cytopathic effect, In certain embodiments, the invention described herein enables the objective analysis of adventitious agents including adventitious viruses, bacteria and mycoplasma.

Abstract: Provided are FRET-based biosensor constructs, and multiplexed platforms or arrays of these biosensor constructs useful for screening candidate drug molecules for efficacy and/or specificity of drug activity. Optionally the biosensor constructs may be located on an inner membrane within a cell or engineered to be located on the cell's surface. The cells or cell lines displaying the biosensors on a cell surface may be arranged as an array of cells for high throughput evaluation of the efficacy and/or specificity of drug candidates, such as a library of candidate drug compounds.

Abstract: A low-variation and highly reproducible method for measuring antibody-dependent cellular cytotoxicity activity of an antibody for a dog as the subject of administration is provided. A method for measuring antibody-dependent cellular cytotoxicity activity comprising a step of causing an antibody and cells in which a canine CD16-canine Fc? receptor ? chain fusion protein is expressed in human-derived NK cells to act on canine tumor-derived cells or cells which express a canine tumor antigen and a step of detecting the cell death of the canine tumor-derived cells or the cells which express the canine tumor antigen. Also, the method for measuring antibody-dependent cellular cytotoxicity activity in which the human-derived NK cells are NK-92 cells.

Abstract: A method for optimal selection of cytotoxic agents for organ or region specific therapy that includes extracorporeal filtration of the cytotoxic agent from blood drained from the organ or region being treated and return of the filtered blood to a mammal is disclosed.

Abstract: Screening methods as well as kits for identifying compounds capable of trapping proteins, e.g. proteins involved in DNA repair, are provided. The methods provide the use of live-cell imaging and local laser micro-irradiation of nuclear DNA in an assay to measure the effects of compounds on the trapping of 5 proteins on DNA. The disruption, e.g. inhibition, of specific proteins, such as poly-(ADP-ribose) polymerases or ALC1 enzyme, leads to trapping on chromatin and/or at DNA damage sites. This inhibits essential cellular functions, e.g. DNA damage repair, and can potentiate cancer cell killing.

Abstract: The present invention relates in general to the field of rapid detection and quantification of tissue/organ injury, and more particularly, to a novel platform for monitoring and quantification of circulating cell-free DNA including within extracellular vesicles, together with extracellular vesicles specific markers, and extracellular vesicles sizes also in combination with PCR-related technologies in biospecimens, and liquid biopsies for the assessment and prediction of severity of tissue/organ injury, monitoring of disease progression as well as the assessment of the response to therapeutic interventions.

Abstract: Aspects of the present disclosure include systems for use in preparing a labelled biomolecule reagent. Systems according to certain embodiments include an input manager for receiving a request for a labelled biomolecule reagent, a memory for storing a dataset having a plurality of labelled biomolecule reagent storage identifiers, a processing module communicatively coupled to the memory and configured to identify one or more labelled biomolecule reagent storage identifiers from the dataset that corresponds to the labelled biomolecule reagent request and an output manager for providing the one or more identified labelled biomolecule reagent storage identifiers. A reagent preparatory apparatus for preparing the labelled biomolecule reagent from an activated biomolecule and activated label is also described. Methods for communicating and receiving a labelled biomolecule reagent request and preparing the subject labelled biomolecule reagents are also provided.

Abstract: Improved biotin blocking methods are provided which utilize a high-affinity monomeric biotin-binding protein of the avidin family that have only a single biotin binding site. Because the biotin-binding protein is monovalent, it can be applied as a single reagent. Since it does not introduce any additional biotin binding sites, as would be the case with native tetrameric streptavidin, no further blocking of biotin binding sites is necessary. Furthermore, because it is a single reagent, it can be combined with other steps such as the peroxidase blocking step, and both endogenous peroxidase and endogenous biotin can be blocked simultaneously. The biotin blocking methods may be used in the performance of immunoassays, such as tissue sample containing endogenous biotin is stained by IHC to detect a specific analyte. Prior to staining, the tissue is first blocked for endogenous biotin, according to the present invention, by the application of a high-affinity biotin-binding protein.

Abstract: Described herein are materials and methods for improved immunoassays using synthetic antigens in which samples are treated to bind antibodies specific to histidine polymer antigens.

Abstract: Herein is reported a method for the determination of the binding affinity of a binder and its ligand comprising the step of determining based on the result of an immunoassay the fraction of free binder in a sample comprising binder, ligand and binder-ligand-complexes for at least two different binder:ligand ratios in the sample, and if the determined fraction of free binder is not comparable for all used binder:ligand ratios then the binder:ligand ratio in the sample is lowered and the sample is re-analyzed by the same immunoassay, and calculating based on the fraction of free binder in the previous step the binding affinity for the binder to its ligand.

Abstract: A portable device for detecting and/or quantifying hemozoin by optical reflectance spectrophotometry, directly on the patient's skin, on tissues or in a liquid sample which comprises means for calibrating the device; at least one optical emitter to excite the sample; at least eight optical detectors to detect the reflectance spectrum of the sample; at least eight bandpass optical filters to filter the reflected light for each optical detector; wherein the optical filters and optical detectors are aligned with each other, wherein the emitter and optical detectors are positioned allowing reflection of the emitted light towards the optical detectors, wherein the optical filters and optical detectors comprise wavelengths between 400 nm and 800 nm; and a microcontroller configured to calculate a ratio between the reflectance values of the sample at each wavelength to detect the reflectance peaks. Also disclosed is a method of detecting and/or quantifying hemozoin by optical reflectance spectrophotometry.

Abstract: A method of classifying an infectious disease in a subject are disclosed. The method comprises: (a) measuring the amount of at least one protein set forth in Table 4 and at least one protein set forth in Table 6 in a sample derived from the subject; and (b) ruling in or ruling out a viral disease based on the amount of the at least one protein set forth in Table 4 and determining the severity of the viral disease based on the amount of the at least one protein set forth in Table 6.

Abstract: The invention relates to methods and kits for detecting one or more antibody biomarkers in a sample, wherein the antibody biomarker specifically binds a viral antigen. In embodiments, the viral antigen is a monkeypox virus (MPXV) antigen, a vaccinia virus (VACV) antigen, or combination thereof.

Abstract: The disclosure provides a method for detecting prostate specific membrane antigen (PSMA) on circulating tumor cells (CTCs) obtained from a patient afflicted with prostate cancer comprising (a) performing a direct analysis comprising immunofluorescent staining and morphological characterization of nucleated cells in a blood sample obtained from the patient to detect circulating tumor cells (CTC), and (b) determining the number of CTCs expressing PSMA.

Abstract: The present invention provides methods and systems for analyzing a biological sample. The methods of the present invention can utilize a labeling moiety and a cleavage moiety. The labeling moiety can comprise a binding moiety that is coupled to a detectable tag via a polynucleotide linker. The cleavage moiety can form a complex with the polynucleotide linker. In some cases, after formation of the complex, the cleavage moiety can effect cleavage of the polynucleotide linker, to release the detectable tag from the binding moiety. The release of the detectable tag can allow one or more additional imaging of the biological sample with a different labeling moiety that comprises a different binding moiety but the same labeling moiety.

Abstract: The subject invention pertains to a new class of compounds, N-acryloylindole (NAIs), and methods of using NAIs as cysteine-reactive probes for proteome-wide cysteine profiling and imaging of thiol oxidative modifications. NAIs are capable of imaging oxidized thiols in cells facing oxidative stress by confocal fluorescence microscopy. NAIs can capture populations of cysteines, particularly those involved in gene expression and regulation.

Abstract: The present invention relates to chemical probes for the improved detection of cysteine in a test sample, preferably an aqueous test sample, as well as respective uses and kits.

Abstract: In certain aspects, the present disclosure is directed to platforms, including methods, devices, and components thereof, for processing samples for mass spectrometry. In other aspects, provided herein are analysis platforms for analyzing mass spectrometry data, including that obtained from mass spectrometry analysis of the samples obtained from the methods and devices described herein. In other aspects, provided are identified proteomic signatures of a condition in an individual, such as a coronary artery disease (CAD) proteomic signature.

Abstract: The present disclosure provides matched gut and peripheral biomarkers to assess, identify and improve therapeutic measures for IBD, in particular ulcerative colitis and Crohn's disease.

Abstract: Provided herein are methods of monitoring for the production of select antibodies in a non-human animal, comprising (a) immunizing a non-human animal with an immunogen; (b) obtaining a blood sample comprising antibody secreting cells (ASCs) from said non-human animal; and (c) individually assaying ASCs present in the blood sample, or a fraction thereof, for the production of select antibodies. Methods of guiding antibody production in a non-human animal for the production of select antibodies are also provided. In exemplary embodiments, the method comprises performing a cycle of (a) to (c), as above, and repeating the cycle when the percentage of ASCs producing select antibodies is below a threshold. In various aspects, the cycle is repeated until the percentage of ASCs producing select antibodies is at or above a threshold. Single cell assays are further provided herein.

Abstract: The present invention provides biomarkers for use in determining populations for treatment with anti-PVRIG antibodies and such biomarkers include, for example PVRIG and/or PVRL2 expression.

Abstract: Provided herein are methods, kits, systems, and compositions or liquid biopsy yield enhancement.

Abstract: Provided are methods, kits and compositions related to toxicity associated with administration of cell therapy for the treatment of diseases or conditions, e.g., cancer, including methods for use in predicting and treating a toxicity. In some embodiments, the toxicity is a neurotoxicity or cytokine release syndrome (CRS), such as a severe neurotoxicity or a severe CRS. The methods generally involve detecting a parameter of a biomarker or individually a parameter of each biomarker in a panel of biomarkers, such as a concentration, amount or activity, and comparing the detected parameter to a reference value for the parameter to determine if the subject is at risk for developing the toxicity, such as neurotoxicity or CRS or severe neurotoxicity or severe CRS.

Abstract: The present invention relates to the field of GPCR structure biology and signaling. In particular, the present invention relates to protein binding domains directed against or capable of specifically binding to a functional conformational state of a G-protein-coupled receptor (GPCR). More specifically, the present invention provides protein binding domains that are capable of increasing the stability of a functional conformational state of a GPCR, in particular, increasing the stability of a GPCR in its active conformational state. The protein binding domains of the present invention can be used as a tool for the structural and functional characterization of G-protein-coupled receptors bound to various natural and synthetic ligands, as well as for screening and drug discovery efforts targeting GPCRs. Moreover, the invention also encompasses the diagnostic, prognostic and therapeutic usefulness of these protein binding domains for GPCR-related diseases.

Abstract: The present disclosure provides a method of detecting IgG4 and Smad1 in a biological sample from a subject with onset or a risk of onset of diabetic nephropathy.

Abstract: The present invention is directed to methods of detecting SLC34A2 expression in muscle tissue in a sample from a subject comprising providing the sample from the subject; and conducting an assay to detect an expression level of SLC34A2 in the sample.

Abstract: Provided herein are methods, devices, kits, and articles of manufacture for detecting pregnancy-associated plasma protein-A (PAPP-A) in a biological sample, for instance a whole blood or serum sample obtained from a pregnant subject, and estimating gestational age (GA) based on the detection of PAPP-A, such that clinical or personal decisions informed by GA can be made without ultrasound.

Abstract: The instant disclosure relates to methods for assessing pulmonary fibrosis disorder disease status in an individual in need thereof. One aspect of the disclosed methods may comprise: detecting a level of one or more biomarkers in a biological sample obtained from an individual, comparing the level of the one or more biomarkers to that of a control value corresponding to the one or more biomarkers, characterizing the disease status in the individual based on the level of the one or more biomarkers as compared to that of a relevant control value; and administering a treatment to said individual based on the assessment of the one or more biomarker levels.

Abstract: The present invention relates to methods and compositions for specifically and quantitatively detecting galectin-3 in a sample. Embodiments of the invention include a detection assay in which a capture binding moiety and a labeled binding moiety specifically recognize non-overlapping epitopes on the N-terminus of galectin-3. Further embodiments are directed to a method for establishing ranges of galectin-3 concentrations indicative of the presence and severity of heart failure in a subject and a method for predicting the clinical outcome of a subject based upon galectin-3 concentration.

Abstract: Glycosylphosphatidylinositol (GPI) is a glycolipid that anchors more than 150 proteins to the cell surface. Pathogenic variants in several genes that participate in GPI biosynthesis cause inherited GPI deficiency (IGD) disorders. Here, the inventors reported that homozygous null alleles of PIGG, a gene involved in GPI modification, are responsible for the rare Emm-negative blood phenotype. Using a panel of K562 cells defective in both the GPI-transamidase and GPI remodeling pathways, they demonstrate that the Emm antigen, whose molecular basis has remained unknown for decades, is carried only by free GPI and that its epitope is composed of the second and third ethanolamine of the GPI backbone. Importantly, the inventors show that the decrease in Emm expression in several IGD patients is indicative of GPI defects. Overall, our findings establish Emm as a novel blood group system and have important implications for understanding the biological function of human free GPI.

Abstract: The present disclosure relates to, inter alia, materials, and methods for detection of infection. More particularly materials, and methods for detecting an infection in a subject's urine or wound exudate are described, e.g. by electrochemically measuring a target molecule and/or a metabolic activity associated with infection using an electrochemical sensor array.