Abstract: A method for analyzing a liquid liable to contain an analyte comprises acquiring a digital image of an area to be analyzed using an image-capturing device having an optical axis directed toward the area to be analyzed, the digital image being exposed for an exposure time and exhibiting a spatial variation in intensity that corresponds to a detection pattern when the analyte is present in the sample. The digital image is processed to identify the spatial variation in intensity therein. The medium to be analyzed is placed in a magnetic field referred to as the “illumination” magnetic field during at least some of the exposure time, the illumination magnetic field being produced by an illumination magnetic source. The illumination magnetic field lies parallel to the optical axis of the image-capturing device over at least some of the area to be analyzed. A device is configured for performing such a method.

Abstract: A system for the evaluation of respirator materials includes an aerosolized particle source configured to selectively provide aerosolized particles, a mixing chamber including a drying apparatus, a vacuum source configured to control airflow, an airtight respirator chamber for evaluating respirator materials, a processor, and a memory. The airtight respirator chamber includes a first sub-chamber, a second sub-chamber, a first sensor and a second sensor. The memory includes instructions stored thereon, which when executed by the processor cause the system to: obtain the first signal and the second signal; determine an aerosol filtration efficiency based on a difference between the first signal and second signal; compare the aerosol filtration efficiency to a predetermined threshold; and provide an indication whether the aerosol filtration efficiency is above or below the predetermined threshold.

Abstract: A device may include a fluid region. A device may also include a magnetochemical sensor for detecting magnetic particles in the fluid region, wherein the magnetochemical sensor comprises: a first ferromagnetic layer, a second ferromagnetic layer, and a spacer layer situated between and coupled to the first ferromagnetic layer and the second ferromagnetic layer. A device may further include a current-carrying structure for drawing the magnetic particles in the fluid region toward the magnetochemical sensor, wherein: the current-carrying structure consists of a single, undivided structure, and the current-carrying structure is configured to carry a current in at least one direction that is substantially parallel to an in-plane axis or a longitudinal axis of the magnetochemical sensor. The magnetochemical sensor may be one of a plurality of magnetochemical sensors in a sensor array.

Abstract: A pore device is used with a measurement device. The pore device includes a pore chip and a chip case which has a chamber partitioned by the pore chip. A measurement terminal group is provided to apply an electric signal from the measurement device to the chamber and output an electric signal generated in the chamber to the measurement device. Interface means is connected to a nonvolatile memory such that the nonvolatile memory is accessible from an outside of the pore device.

Abstract: Aspects of the present disclosure include methods, systems and non-transitory computer readable storage mediums for classifying cytometric image data using single particle, e.g., cell, images. Methods of classifying cytometric image data according to certain embodiments include: receiving unclassified cytometric image data, wherein the cytometric image data comprises a plurality of images corresponding to image channels, and iteratively modulating aspects of at least one image of the plurality of images of the cytometric image data and applying a model to the modulated cytometric image data to classify the cytometric image data, wherein the model is trained to estimate the presence of a particle belonging to a first category of particles in the cytometric image data.

Abstract: To provide a technology of maintaining light detection accuracy at a high level irrespective of individual variations in flow rate of microparticles flowing through a flow channel. The present technology provides a microparticle measuring apparatus including: a plurality of light detection sections configured to detect, at different positions, optical information emitted from microparticles flowing through a flow channel; and a detection timing control section configured to control a detection timing of each light detection section, on the basis of a trigger signal detected at a first reference channel provided in a first light detection section, and an optical signal detected at a second reference channel provided in a second light detection section that detects optical information emitted from the microparticles, at a position different from a position of the first light detection section.

Abstract: A biological information measuring technique with improved reliability is proposed. A method of measuring biological information using an optical element with a totally reflecting surface, which is to be brought into contact with an object to be observed, is provided. The method includes, after the optical element has been in contact with the object to be observed, acquiring an absorbance at the totally reflecting surface in a state where the optical element is not in contact with the object to be observed, and determining by an information processing apparatus whether the object to be observed needs to be cleaned, based on the absorbance.

Abstract: A system and method for performing nanoscale thermal property characterization of materials. The system combines the operating principles of thermoreflectance-based techniques and scanning probe microscopy techniques into a hybrid solution capable of deriving thermophysical properties of a sample. A pump laser beam heats a distal end of a cantilever, and a probe laser beam is reflected off of a specular surface at the distal end of the cantilever carrying with it thermoreflectance data that can be used to extract thermophysical properties of the sample region adjacent to a tip suspended at the distal end of the cantilever.

Abstract: A sensing device includes an optical waveguide substrate and a plurality of biological probes. The optical waveguide substrate includes a light input end and a light output end. A plurality of biological probes are arranged and aligned on the optical waveguide substrate along a direction from the light input end to the light output end. The plurality of biological probes respond to different resonance wavelengths along the direction corresponding to different analytes. The present disclosure further includes a biosensing system including the sensing device.

Abstract: Aspects of the subject disclosure may include, for example, generating one or more first optical signals, launching the first optical signal into a medium, receiving from the medium one or more second optical signals, mixing the one or more second optical signals with one or more third optical signals to generate one or more mixed signals, obtaining one or more electrical signals from the mixed signal, and generating one or more point spread functions from the one or more electrical signals. Other embodiments are disclosed.

Abstract: An optical system (31) for analysing biological processes comprising: a biological process vessel (33) comprising a chamber and an optical interface element (34) for optically coupling the chamber to an optical emitter, a wavelength discrimination component, and an optical detector when a biological process fluid in the chamber is being analysed, the optical interface element being configured to couple light in the mid-infrared range; an optical emitter (351) configured to emit light in the mid-infrared range; an optical detector (353) configured to detect light in the mid-infrared range; a wavelength discrimination component (352); an alignment component (42) for aligning the optical emitter, optical detector, and wavelength discrimination component with the optical interface element; and a processor (354) configured to: receive outputs from the optical detector; and process the received outputs from the optical detector to provide an indication of the constituents of a biological process fluid in the chambe

Abstract: Provided is a method for determining the resin penetration into at least one porous coating material which is pressed with at least one carrier board and at least one resin layer arranged on the carrier board. During the pressing process the resin penetrates or rises into the at least one porous coating material.

Abstract: A substrate processing apparatus includes a supply channel through which a liquid to be supplied to a substrate flows; and a foreign substance detecting unit configured to detect a foreign substance in the liquid based on a signal obtained when light, which is near-infrared light, is radiated toward a flow path forming unit constituting a part of the supply channel by a light projector so that light is emitted from the flow path forming unit and a light receiver receives the light emitted from the flow path forming unit.

Abstract: A gadget for retroreflected signal measurement is disclosed. The gadget for measuring the retroreflected signal includes a partitioning wall; a portable terminal receiving portion disposed on one surface of the partitioning wall; a biosensor receiving portion disposed on the other surface of the partitioning wall and constructed to receive a biosensor therein; a light exit channel defined in the partitioning wall and at one side thereof; and a light-receiving channel adjacent to the light exit channel and defined in the partitioning wall, wherein light passes through the light exit channel and then is reflected from the biosensor, and then is incident into the light-receiving channel.

Abstract: A microfluidic ion detection chip having a bubble brightening structure includes a substrate, an upper plate, two glass cover plates, and a foam board. The substrate is provided with a fluid mixing region, a lower optical detection through hole, and a lower gas flow channel. The upper plate is adhesively connected to the substrate and is provided with a sample outlet, an upper optical detection through hole, an upper gas flow channel, and two sample inlets, the upper gas flow channel and the lower gas flow channel are combined to form a gas flow channel. The two glass cover plates are respectively disposed at the lower optical detection through hole and the upper optical detection through hole. The foam board is provided with a foaming structure capable of generating bubbles and a surfactant coated on the foam board, the foam board is disposed at the gas flow channel.

Abstract: A system for identification of a biological structure present in a liquid biopsy sample is provided. The system identifies common biological structures and rare biological structures based on their fluorescence characteristics and morphology. The identified biological structures may be used in diagnosis and treatment of a human afflicted with a disease. Examples described in this disclosure also relate to methods and assays that may be used together with the systems of this disclosure for diagnosis and treatment of a human afflicted with a disease.

Abstract: Disclosed is an autofluorescence quenching device that: quenches only autofluorescence without quenching fluorescence that is originally desired to be observed in a tissue section in a short time; does not emit noise in various wavelength regions; is capable of controlling a temperature increase; can be used repeatedly with a simple operation; and can stably quenches autofluorescence. The autofluorescence quenching device for a sample includes: a sample placement unit; and an irradiation unit mounted on the sample placement unit. The sample placement unit includes: a flat plate; one or more sample placement recesses each in which a sample is to be placed and which are provided in the flat plate; and a cooling means for cooling the sample placement recesses. The irradiation unit includes one or a plurality of white LED illuminations placed above the respective sample placement recesses.

Abstract: Methods and apparatus for measuring a surface charge distribution and/or electrical properties of entities in a liquid are disclosed. In one arrangement, a liquid (2) is provided in contact with a target surface (4). The liquid (2) contains charged entities (6). A concentration of the charged entities (6) in a region between a probe member (8) contacting the liquid (2) and the target surface (4) is detected at each of a plurality of different positions on the target surface (4). A surface charge distribution on the target surface (4) is determined using the detected concentrations.

Abstract: A method for assessing fluorescence imaging agent-based visual enhancement in a color image in which a fluorescence imaging agent signal is represented by a first color, the method including: identifying at least one first region of the color image that is encompassed by a portion of anatomy or pathology of interest in which the fluorescence imaging agent is present; identifying at least one second region of the color image that is not encompassed by the anatomy or pathology of interest; and determining a measure of visual enhancement of the anatomy or pathology of interest in the color image by comparing a contribution of the first color relative to other colors in the at least one first region to a contribution of the first color relative to the other colors in the at least one second region.

Abstract: An optical stack for sensing a presence of an analyte is provided. The optical stack includes a sensor material. The sensor material includes a first optical response including a first optical property having a second value in response to an excitation signal including the first optical property having a first value different from the second value. The first optical response includes a second optical property sensitive to the presence of the analyte. The optical stack includes a first optical film disposed proximate the sensor material and includes a third optical property having respective third and fourth values in response to the respective first and second values of the first optical property. The third value is different from the fourth value by at least a factor of 2.

Abstract: The present invention provides, among other things, devices, kits, apparatus, and methods for rapid homogenous cell staining and imaging. Particularly, in some embodiments, the present invention can immunochemically stain a cell or a tissue in less than 60 seconds without washing. In some embodiments, the present invention stains and observes analyte (protein or nucleic acid) inside a cell in 60 seconds without washing.

Abstract: A surface plasmon resonance imaging apparatus is provided. The surface plasmon resonance imaging apparatus includes a light irradiation unit configured to irradiate polarized light onto a metal coating film provided on one surface of a prism, a light modulator configured to spatially pattern-encode light reflected by the metal coating film and the prism, a light detector configured to detect a pattern-encoded light signal, obtained through pattern-encoding by the light modulator, as a spectral signal, a signal processor configured to spatially decode the spectral signal and analyze a decoded spectral signal to generate characteristic data of a sample provided on the metal coating film, and an output unit configured to output the characteristic data of the sample as a two-dimensional (2D) image.

Abstract: A diffractometric sensor (1), comprises:—a substrate (3);—two interdigitated affinity gratings (2), a first affinity grating (20) comprising first unit cells (200) with affinity elements (201) and a second affinity grating (21) comprising second unit cells (210) with affinity elements (211), wherein the first unit cells (200) and second unit cells (210) are configured and arranged such that coherent light of a predetermined wavelength generated at a predetermined beam generation location (40) and diffracted by target molecules (204, 214)) bound to the affinity elements (201, 211) constructively interferes at a predetermined detection location (50) with an inverse phase, and wherein the first and second affinity gratings (20, 21) are balanced to generate a bias signal at the predetermined detection location (50) that corresponds to a difference (Am) in the scattering mass of the first and second affinity gratings (20, 21) which is in the range of 0.001 pg/mm2 to 30000 pg/mm2.

Abstract: Systems and methods are described herein for waveguide defect detection. In some examples, a statistical value of pixels of a number of image locations in one or more images of the waveguide can be computed. An extremity matrix can be generated based on an anomaly detector model and statistical pixel data that includes statistical values computed for respective image locations in the one or more images of the waveguide. A decision can be made to determine whether the waveguide is a conforming or a non-conforming waveguide based on the extremity matrix and a set of thresholds. In some examples, the statistical value is a mean intensity value or an average intensity value.

Abstract: A semiconductor measurement apparatus includes an illuminator configured to output light having a first wavelength band and light having a second wavelength band, different from the first wavelength band, a stage on which a test object is positioned, a camera configured to receive light reflected or scattered from the test object or transmitted through the test object, and a controller configured to control the illuminator and the camera, and to measure, based on information indicated by the light received by the camera, a plurality of structures included in the test object. The controller is configured to set an exposure time of the camera to a first exposure time while the illuminator outputs the light having the first wavelength band, and to set the exposure time of the camera to a second exposure time, different from the first exposure time, while the illuminator outputs the light having the second wavelength band.

Abstract: Automatic detection of mechanical defects in piece parts, such as floor covering planks, enables removal of the defective parts off an inspection path. A bypass conveyor system enables defective parts to be transported beyond or around a manual inspection, enabling personnel performing the subsequent inspection to focus on appearance defects. Once the remaining parts have been inspected by a manual process, the parts transported by the bypass conveyor system are returned to the production line. Defective parts may be distinguished by a marking capability that provides the ability to mark according to a variety of classes of defects.

Abstract: The invention relates to a method for analyzing a crop sample comprising a target plant material with soil tare adhered thereto, in particular soiled plant material. Further, the invention relates to a method for generating first calibration data for analyzing a crop sample comprising a target plant material with soil tare adhered thereto, and an analysis assembly for analyzing a crop sample comprising a target plant material with soil tare adhered thereto. In addition, the invention relates to an arrangement for analyzing a crop sample comprising a target plant material with soil tare adhered thereto, a sugar production facility, and the use of an analysis assembly in a sugar production facility. Further, the invention relates to a method for determining components in sugar beets for sugar production. Further, the invention relates to an arrangement for determining components in sugar beets, and to a sugar production facility.

Abstract: The present invention relates to a macro plastic and micro plastic detection method based on RGB and hyperspectral image fusion, which includes the following steps: obtaining macro plastics and micro plastics; mixing with solid wastes to obtain a solid-phase substrate; pretreating the obtained solid-phase substrate to obtain a material; drying to remove part of moisture and coating on a quartz window sheet, drying until the moisture is completely removed, and flattening by using another quartz window sheet to obtain a material to be detected; obtaining an RGB image and a hyperspectral image of the material to be detected respectively by using a high-resolution color image scanner and a hyperspectral camera; fusing the obtained RGB image and hyperspectral image; and automatically classifying and identifying the macro plastics and the micro plastics by using a supervised classification model.

Abstract: A method includes directing light at a first side of a semiconductor structure; detecting a first light intensity at a second side of the semiconductor structure, wherein the first light intensity corresponds to the light that penetrated the semiconductor structure from the first side to the second side; and comparing the first light intensity to a second light intensity, wherein the second light intensity corresponds to an expected intensity of light.

Abstract: An apparatus for sampling a fluid from a flowline comprises a housing and a pressure control barrier moveably mounted in the housing. The apparatus comprises a sample chamber within the housing on a first side of the pressure control barrier and being fluidly connectable to the flowline for receiving a fluid sample from the flowline, and a pressure control chamber within the housing on an opposite second side of the pressure control barrier, wherein pressure applied within the pressure control chamber controls pressure of the fluid sample within the sample chamber. The apparatus comprises a guided wave radar device for determining a property of the fluid sample within the sample chamber.

Abstract: The device includes a first X-ray source, configured to illuminating a measurement cell with a first beam of X photons; a first detector, placed opposite the first X-ray source along a first illumination axis; a second X-ray source, configured to illuminating the measurement cell with a second beam of X photons simultaneously with the first X-ray source; a second detector, placed opposite the second X-ray source along a second illumination axis; and a tray carrying the first X-ray source, the first detector, the second X-ray source, and the second detector, the tray being rotatable around the cell axis.

Abstract: Provided is an XPS sample holder including: a holder body configured to provide an inner space; a first voltage transmitting member that passes through a top surface of the holder body in a vertical direction; a second voltage transmitting member that passes through the top surface of the holder body in the vertical direction and is spaced apart from the first voltage transmitting member in a first direction that is a horizontal direction; an inner connection member disposed in the holder body and electrically connected to the first voltage transmitting member; a first electrode part disposed on the top surface of the holder body and connected to the first voltage transmitting member; and a second electrode part disposed on the top surface of the holder body and connected to the second voltage transmitting member.

Abstract: Systems and methods for interpreting high-energy interactions on a sample are described in this application. In particular, this application describes an analysis method that comprises impinging radiation from a source on an analyte, detecting the energy interactions resulting from the impinging radiation using a radiation detector, adjusting the signal from the radiation detector using a machine learning module to emphasize specific parts of the detector signal, training the machine learning module in a supervised or unsupervised manner, producing quantitative and qualitative models using the machine leaning module, and then applying the machine learning module to additional energy interactions. The signal received by the detector can be preprocessed to emphasize specific parts of the detector signal, which is then mapped to a machine learning module for training in a supervised or unsupervised manner.

Abstract: Methods and systems include generating, from an electron beam generator, an electron beam in a vacuum chamber. A mounting platform in the vacuum chamber is configured to support a material. The electron beam is directed at a surface region of the material at a grazing angle. A detector assembly, which may have an optical entry path positioned above the surface region, receives cathodoluminescent light emission arising from the electron beam transferring energy to the surface region. The detector assembly determines spectral characteristics of the cathodoluminescent light emission to characterize the surface region.

Abstract: A defined peak region residing between about 3.2 and 3.4 ppm of a proton NMR spectrum of an in vitro biosample is electronically evaluated to determine a level of trimethylamine-N-oxide (“TMAO”). The biosample may be any suitable biosamples including human serum with a normal biologic range of between about 1-50 ?M or urine with a normal biologic range of between about 0-1000 ?M.

Abstract: The invention teaches a method proposing two new indices for evaluating thermal evolution in oils from different basins and organofacies. The first index is based on the distribution ratio of high molecular weight sulfur compounds, belonging to the DBE 6 (benzothiophene) and DBE 9 (dibenzothiophene) series. The second index, called TEI, was created from the profile of aromatic hydrocarbons and molecules containing N, O and S. Both parameters were obtained from the direct characterization of the oils, by using the photoionization at atmospheric pressure (APPI) technique combined with Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS).

Abstract: A method for determining a value representative of a state transition temperature of a resist structure, formed of a resist material and having predetermined dimensions, on an underlayer material includes: receiving data earlier obtained, the data representing a correlation between a second value for a measure representative of a spatial feature of at least one resist structure of each of a plurality of entities after applying a heat treatment, and a temperature at which the heat treatment is applied, each entity comprising the at least one resist structure, formed of the resist material and having the predetermined dimensions before the heat treatment, on the underlayer material, and wherein the measure has a first value before the heat treatment, and determining, from the correlation, the value representative of the state transition temperature when the heat treatment would be performed at such temperature, the second value differs by a predetermined amount from the first value.

Abstract: A sag detection apparatus comprises an oven containing a sample cell supported by a cell support structure, a thermal conductivity sensor including a sensor housing, and a roller with a first end supported by a first bearing and fixedly coupled to a first end of the cell support structure and a second end supported by a second bearing and fixedly coupled to a second end of the cell support structure. Temperature sensor wires electrically connect a temperature sensor and first fixed contact via stationary contacts configured to remain fixed during rotation of the roller and rotating contacts configured to rotate with rotation of the roller. Heat source wires electrically connect a heat source and a second fixed contact via stationary contacts configured to remain fixed during rotation of the roller and rotating contacts configured to rotate with rotation of the roller.

Abstract: The present disclosure provides a test system and a method for measuring efficiency of underground coal gasification, and relates to the technical field of underground coal gasification, comprising a gasification agent supply system, a gasifier, an outlet and recycling system and a monitoring system, wherein the gasification agent is mainly injected into the gasifier and then chemically reacts with the coal by igniting the gasifier at a high temperature to generate gas, while after the gas passes through the outlet and recycling system, the composition and content are measured, and the monitoring system monitors an operating state of the gasifier, injection of the gasification agent and production of gas, temperature change in the gasification channel and ignition temperature and pressure gauge data.

Abstract: The apparatus is attached to a thermal analyzer including a pair of sample containers and a heating furnace having a window or an opening through which at least the measurement sample can be observed, and includes an attachment unit, a light source, a polarizer constituting a polarizing filter that polarizes irradiation light emitted from the light source, a camera, a half mirror, a rotation mechanism and an analyzer constituting a polarizing filter configured to polarize reflected light through the half mirror and to introduce the resulting polarized light of the reflected light into the camera, in which the polarized light irradiates the sample after passing through the window or opening and then reflects from the sample wherein the analyzer, the half mirror, and the light source are fixed to a fixing member and the rotation mechanism rotates the fixing member in a polarization direction of the analyzer.

Abstract: The present disclosure is directed towards a method, system, and apparatus for distinguishing between isotopically substituted liquids, such as H2O and D2O, and sensing other isotopologues of water, such as H218O. In an embodiment, the electrical output characteristics of the apparatus or device are used as diagnostic signals to distinguish between isotopic compositions and isotopologues of a liquid. In an embodiment, water droplets with volume of several micro litre are applied to the apparatus or device generating some electrical signals which show differences between the application of H2O and D2O, for example. One advantage of the present method and system is that the configuration is simple and portable, with no need for complicated equipment and operation protocols.

Abstract: A flexible sensor device for moisture detection, a detecting method using said device, and applications of the device are disclosed.

Abstract: There is presented a method of measuring one or more potential differences being indicative of one or more concentrations of one or more analyte ions in a sample, such as being a liquid whole blood sample, said method comprising, measuring with a reference ion measurement setup a parameter indicative of a concentration of a reference ion in the sample wherein the reference ion measurement setup is different from an electroanalytical measurement setup, measuring with an analyte ion measurement setup one or more potential differences directly or indirectly between each of one or more, optionally solid-state, working electrodes with each of said, optionally solid-state, working electrodes comprising an ion-selective electrode which is selective for an analyte ion, and an, optionally solid state, reference electrode which is selective for the reference ion wherein the analyte ion measurement setup is an electroanalytical setup. There is additionally presented an apparatus and use of said apparatus.

Abstract: A new class of sensing elements made of a protein pore fused to a programmable antibody-mimetic binder. This modular design expands the utility of nanopore sensors to numerous proteins while preserving their architecture, specificity, and sensitivity. The sensing elements were validated with protein analytes that drastically vary in size, charge, and structural complexity. These analytes produce unique electrical signatures that depend on their identity and quantity and the binder-analyte assembly at the nanopore tip.

Abstract: Provided herein are methods of detecting target molecules using electrochemical sensors that comprise biomolecular receptor-bound redox reporters. Related systems and computer readable media are also provided.

Abstract: The disclosed subject matter relates to techniques for methods and systems for non-reagent chloride analysis in an acid copper plating bath, using a blend of VMS (Virgin Makeup Solution) to generate Ag+-containing solution as a titration into a sample.

Abstract: A gas sensor (1) including a sensor element (10); a metallic shell (138); a tubular outer surface member (190) attached directly or indirectly to the metallic shell, constitutes an outer surface of the gas sensor, and extends toward a rear end side in relation to the metallic shell; and a tubular protection member (210, 220) which is detachably fixed to a fixation region C1, C2 of an outer circumference of the outer surface member. The protection member includes a first arc portion (212), a second arc portion (222), and a fixation portion (214,224) brought into contact with and is fixed to the fixation region. When the protection member fixed to the outer circumference of the outer surface member by fixing the fixation portion to the fixation region is viewed in the axial O direction, the first arc portion and the second arc portion cover in total at least 90% of the entire circumference of the outer surface of the outer surface member.

Abstract: A structure includes a cavity in a semiconductor substrate; a field effect transistor positioned over the cavity; an opening in the semiconductor substrate extending to the cavity; and a layer of insulating material filling the opening and forming an insulating material window to the cavity.

Abstract: A sensor component includes a sensor including a sensor surface and a reaction site in cooperation with the sensor and exposing the sensor surface. The reaction site including a reaction site surface. A surface agent is bound to the reaction site surface or the sensor surface. The surface agent includes a surface active functional group reactive with Bronsted base or Lewis acid functionality on the reaction site surface or the sensor surface and including distal functionality that does not have a donor electron pair.

Abstract: The present disclosure provides a particle analyzer and a particle test control method and device thereof. A method comprises, acquiring a blood sample in a test location; preparing a diluted sample by the acquired sample; after acquiring a diluted sample, monitoring whether a pore blocking event occurs during a counting process; when the pore blocking event occurs, suspending the test of the sample, and performing an unblocking operation; and after the unblocking operation is completed, re-counting the same diluted sample without re-acquiring and re-diluting the blood sample by the impedance method after the unblocking operation.