Abstract: Disclosed herein include systems, methods, compositions, and kits for quantitative analysis of a plurality of cellular component targets of cells of interest. In some embodiments, an oligonucleotide associated with a cellular component-binding reagent (e.g., an antibody) is associated with one or more detectable moieties (e.g., luminescent moieties, fluorescent moieties, a phosphorescent moieties). In some embodiments, the presence of the detectable moiety in the binding reagent oligonucleotide enables the cellular component-binding reagent to be employed for both fluorescence analysis (e.g., cell sorting) and sequence analysis (e.g., protein expression profiling).

Abstract: One aspect of the invention is a method for amplifying alpha globin genes HBA1, HBA2 and HBA12 in a single PCR tube to determine an HBA genotype of a subject. This method employs five primers selected to accurate and sensitively identify the HBA1, HBA2, and HBA12, a gene found at a higher frequency in citizens of Saudi Arabia, by accurately annealing to nucleic acids in a biological sample and simultaneously amplifying sequences encoding the alpha globin genes. This invention includes a procedure and required reagents for the amplification of alpha globin genes in a single PCR tube.

Abstract: A fluidic device, instrument, and method for processing a biological sample is disclosed herein. An example of the fluidic device includes structures (e.g., sample holder(s), reaction chamber(s), reagent storage zone(s), metering compartment(s), reaction well(s), detection well(s), channel(s), etc.) for efficiently processing sample components and providing expression levels of various biomarker components for characterization of a sample.

Abstract: SARS-CoV-2 infection in children is generally milder than in adults, yet a proportion of cases result in hyperinflammatory conditions often including myocarditis. To better understand these cases, the inventors applied a multi-parametric approach to the study of blood cells of 56 children hospitalized with suspicion of SARS-CoV-2 infection. The most severe forms of MIS-C (multisystem inflammatory syndrome in children related to SARS-CoV-2), that resulted in myocarditis, were characterized by elevated levels of pro-angiogenesis cytokines and several chemokines. This phenotype was associated with TNF-? signaling, sustained NF-?B signaling in monocytic/dendritic cells, alongside increased HIF-1? and VEGF signaling.

Abstract: Described herein is a method for detecting cancer DNA in a test sample of DNA from a patient. In some embodiments, the method may comprise: (a) sequencing multiple aliquots of the test sample to produce, for each aliquot, sequence reads corresponding to two or more target regions that each have one or more sequence variations present within the patient's cancer; (b) for each aliquot, for each target region: i. determining the number of sequence reads that have the sequence variation; ii. determining the total number of sequence reads; and iii. comparing i. and ii. to one or more error probability distribution models for the sequence variation, wherein the one or more models are obtained from DNA that does not contain the sequence variation; and (c) integrating the collective results of step (b) to determine if there is cancer DNA in the test sample.

Abstract: The invention is directed to biomarkers for predicting a patient's response, both therapeutic and toxic, to immunotherapy.

Abstract: Embodiments of a computing device and methods of providing a cancer assessment for a patient are described. The method involves isolating a volume of a fluid from a fluid sample of the patient, the volume of fluid including at least one biomarker; adding at least a portion of the volume of fluid to a nanosensor comprising nanoparticles configured to capture the at least one biomarker and amplify signals emitted by the at least one biomarker during Raman spectroscopy; performing Raman spectroscopy on the volume of fluid on the nanosensor to produce a sample Raman spectrum having amplified signals indicating the presence of the at least one biomarker on the nanosensor; processing the sample Raman spectrum using data from template Raman spectra from known cancer samples; and based on the detected one or more cancer characteristics, providing the cancer assessment of the patient.

Abstract: Methods for the determination of the risk for developing a head and neck cancer are described. The method is based on the determination of the methylation status of genomic DNA sequences located between the genes ZNF823 and ZNF833, wherein when the sequences are methylated the risk for developing a head and neck cancer is increased.

Abstract: This disclosure relates to new assay methods for analysis of circulating tumor cells (CTCs) in blood samples for detection, e.g., early detection, and/or monitoring of disease, e.g., cancer. The methods provide ultra-high sensitivity and specificity, and include the use of microfluidic isolation of CTCs and digital detection of RNA derived from the CTCs.

Abstract: The invention relates generally to immortalized libraries, also referred to as archived reference samples, and their use in diagnostic methods.

Abstract: Described are methods of treating human papillomavirus (HPV) type 16- or HPV type 18-related High-grade Squamous Intraepithelial Lesion (HSIL) of the cervix. The described methods comprise evaluating one or more biological samples for the presence of miRNAs and isomiRs thereof.

Abstract: The invention provides in vitro methods of determining whether an individual has a pre-cancer or cancer comprising determining the presence or absence of one or more methylation markers of a methylation marker set in a urine sample of said individual; and determining whether the individual has pre-cancer or cancer based on the detection of the presence or absence of said one or more methylation markers in the urine sample, wherein the presence of said one or more methylation markers indicates that the individual has pre-cancer or cancer. The invention further provides methods for typing pre-cancer or cancer based on the presence or absence of one or more methylation markers of a methylation marker set in a urine sample.

Abstract: Disclosed herein are methods of identifying a cell type by its genomic methylation pattern from a biological sample of a human subject. In some cases, the method can comprise identifying sperm and/or prostate cells. Also described herein are methods of identifying cell types for forensic analysis and for the treatment of a disease. The methods disclosed herein may comprise determining the DNA methylation pattern of DNA extracted from a semen sample. Also disclosed herein are methods of treating a disease or condition of a subject.

Abstract: Complement components form a plasma innate immune cascade but could also serve as multitasking proteins, as they have functions beyond this system. Here, we show that complement FH is locally expressed by multiple types of human tumors. We provide a paradigm shift for the impact of FH on cancer progression, showing a previously unrecognized, intracellular function of FH outside the complement cascade, while the canonical, complement-regulatory function had no effect. Int-FH served as a driver of the proliferation and migration of ccRCC and lung ADK cells but not of normal cells or lung SCC cells. The presence of int-FH staining in tumor cells indicated poor prognosis for ccRCC and lung ADK.

Abstract: The present disclosure relates to methods of screening tilapia for increased genetic resistance to viral infection, such as Tilapia Lake Virus, as well as the use of these fish, which have been identified as having increased genetic resistance, in aquaculture breeding programs and production.

Abstract: The subject matter disclosed herein is generally directed to stratifying and treating coronavirus infections based on intrinsic immune states.

Abstract: Methods and a kit for detection of genetic material from SARS-CoV-2 that combines reverse transcription loop-mediated isothermal amplification (RT-LAMP) technology with specific oligonucleotide primers, fluorophore-labeled oligonucleotides, quencher technology, buffer components, enzymes, and enzyme ratios chosen to minimize false positive and false negative results, are described. The method includes internal positive control targeting sequences, allowing more certain interpretation of the results. The reaction can be performed at a single elevated temperature, can be completed in 1-2 hours, and the results can readily be interpreted by visually observing the fluorescence color of the reaction using ultraviolet light.

Abstract: Described herein is a method of detecting secondary infection in a patient, particularly a patient with a primary infection that is a pneumonia, a COVID-19 infection, or a COVID-19 pneumonia. In some cases, the secondary infection is a secondary bacterial infection, e.g., secondary bacterial pneumonia. In some cases, the methods provided herein detect a hyper-inflammatory response or severity of disease, e.g., indicating a severe COVID-19 infection, or provide a risk of death from a disease (e.g., COVID-19). This disclosure also provides method of detecting a localized respiratory infection in a subject by quantifying microbial cell-free nucleic acids (e.g., mdfDNA) from plasma from the subject. In some cases, the subject is not bacteremic when plasma is collected from the subject. This disclosure also provides systems, such as nucleic acid-sequencing systems with increased reliability for detecting secondary infections, particularly in patients with culture-negative pneumonia.

Abstract: A set of primers and probes for simultaneous detection of Cymbidium mosaic virus (CymMV), Odontoglossum ringspot virus (ORSV), and Cymbidium ringspot virus (CymRSV) and a method for detecting CymMV, ORSV, and CymRSV, along with a method for their detection, are disclosed. The method involves designing multiplex real-time quantitative PCR detection primers and probes for CymMV, ORSV, and CymRSV and applying these primers and probes to the real-time quantitative PCR simultaneous detection of CymMV, ORSV, and CymRSV. It allows for faster detection of CymMV, ORSV, and CymRSV, taking only one-third of the time compared to uniplex real-time quantitative PCR technology, thereby reducing testing costs by approximately ? to ½ for each sample. The primers and probes are highly specific and sensitive, with a sensitivity as low as 1 to 10 copies. It provides an efficient and feasible detection method for early detection and prevention of CymMV, ORSV, and CymRSV.

Abstract: A method for the treatment of black slag, which contains lime (CaO) or lime-based compounds, and which is generated in an electric furnace that produces steel from scrap, includes indirectly cooling the black slag immediately after the black slag exits from the electric furnace or after collection of the black slag in a cauldron, for a period of time between about 10 minutes and about 45 minutes.

Abstract: A method to manufacture a steel product in a steelmaking plant including several different tools, the method including the definition of at least two manufacturing routes using different tools and the calculation of the expected level of CO2 emissions associated to each of this defined manufacturing routes.

Abstract: A residual molten material amount detection method and detection apparatus that can detect a residual amount of molten material in a vertical furnace and a method for operating a vertical furnace by using the detection method. The residual molten material amount detection method detects a residual amount of molten material remaining in a bottom portion of a vertical furnace after end of discharge of the molten material. The method includes detecting the residual amount of the molten material by using a difference between a production speed of the molten material and a discharge speed of the molten material that is calculated by using a discharge acceleration, a discharge period, and an initial discharge speed of the discharge of the molten material through a taphole.

Abstract: A method to manufacture a global tonnage of steel products in at least two steelmaking units wherein expected level emissions are calculated and compared with pre-defined targets.

Abstract: A method of manufacturing a steel product into at least two different steelmaking units wherein an expected level of CO2 emissions for the manufacturing of said product in each respective steelmaking unit is calculated.

Abstract: The invention is a nitriding treatment method for a steel component including at least two nitriding treatment steps, i.e., a first nitriding treatment step in which a nitriding treatment is performed to a steel component under a nitriding gas atmosphere of a first nitriding potential, and a second nitriding treatment step in which another nitriding treatment is performed to the steel component under another nitriding gas atmosphere of a second nitriding potential lower than the first nitriding potential, after the first nitriding treatment step. The first nitriding treatment step and the second nitriding treatment step is performed at a temperature within a range of 500° C. to 590° C. The first nitriding potential is a value within a range of 0.300 to 10.000. The second nitriding potential is a value within a range of 0.253 to 0.600.

Abstract: A steel sheet has a chemical composition containing, by mass %, C: 0.20% or more and 0.40% or less, Si: more than 1.0% and 3.0% or less, Mn: 1.5% or more and 3.5% or less, P: 0.002% or more and 0.010% or less, S: 0.0002% or more and 0.0020% or less, sol. Al: 0.40% or less (not including 0%), and N: 0.0100% or less, with the balance being Fe and incidental impurities; and a steel microstructure containing, in area fraction, tempered martensite: 45% or more and 83% or less, bainite: 15% or more and 53% or less, and retained austenite: 2% or more. Carbides in the tempered martensite have an average particle size of 0.40 ?m or less, an average amount of C in the retained austenite is 0.5% by mass or more, and a tensile strength is 1,470 MPa or more.

Abstract: A steel sheet has a certain chemical composition and a steel microstructure containing, in area fraction, martensite: 40% or more and 78% or less, bainite: 20% or more and 58% or less, and retained austenite: 2% or more. Carbides in tempered martensite in the martensite have an average particle size of 0.40 ?m or less, an average amount of C in the retained austenite is 0.5% by mass or more, a Si concentration in a region within 100 ?m in a thickness direction from a surface of the steel sheet is 1.3% by mass or less, and a tensile strength is 1,470 MPa or more.

Abstract: One aspect of this invention relates to a method for producing a carbide-free bainite and retained austenite steel.

Abstract: A cold roll strip or sheet includes in (wt %) C 0.08-0.28; Mn 1.4-4.5; Cr 0.01-0.5; Si 0.01-2.5; Al 0.01-0.6; Si+Al?0.1; Si+Al+Cr?0.4; Nb?0.008; Ti?0.02; Mo?0.08; Ca?0.005; V?0.02; balance Fe apart from impurities. The steel is within the area defined by the coordinates A, B, C, D, where Ri/t (y-axle) is plotted vs TS(MPa)/YR (x-axle), and where A is [1200, 2), B is [2000, 4], C is [2000, 3], and D is [1200, 1].

Abstract: The present application relates to a method and system for performing intelligent sorting based on dynamic adjustment of a threshold. The method includes: sorting ores with a predetermined granularity by an intelligent sorting system according to a current grade threshold to output the sorted ores; performing grade detection on the fine ores to obtain a current state parameter of the fine ores; calculating a first error rate of a current comprehensive grade based on the current comprehensive grade and a target comprehensive grade, and in a case that the first error rate is not within a set range of a comprehensive error rate, calculating a dynamic adjustment step length for a grade threshold according to the current state parameter of the fine ores; and performing dynamic adjustment according to the dynamic adjustment step length and the current grade threshold to obtain the adjusted current grade threshold.

Abstract: An improved beta(?)-spodumene (˜LiAlSi2O6) nitric acid conversion process produces discrete lithium (Li), aluminum (Al) and silica (SiO2) materials by: (i) converting lithium nitrate, LiNO3, to lithium carbonate, Li2CO3; (ii) creating a Al-rich precipitate either by thermally decomposing aluminum nitrate, Al(NO3)3, or by reacting Al(NO3)3 with aqueous and/or solid ammonium carbonate, (NH4)2CO3; and (iii) forming a solid SiO2-rich aluminosilicate residue by selectively leaching Li and Al from ?-spodumene. Three key reactants consumed during processing—nitric acid (HNO3), ammonia (NH3), and magnesium oxide (MgO)—may be regenerated internally by closed-loop chemical cycles, this feature of the process greatly improving its economics in commercial applications.

Abstract: The present invention relates to a species of genus Pseudomonas identified as Pseudomonas BR87641, termed Candidatus Pseudomonas pretiosorbens, having Accession Deposit Number DSM 33684.

Abstract: Provided are modified bacteria for use in bioleaching rare earth elements (REEs). The modified bacteria contain at least one engineered genetic change that is correlated with improved bioleaching of the REEs, relative to REE bioleaching by unmodified bacteria of the same species as the modified bacteria. Also provided is a method for extracting REEs by contacting a composition containing REEs with biolixiviant produced by the modified bacteria. Kits that include containers that hold the modified bacteria are also provided.

Abstract: The invention provides a method for the recovery of a metal-containing product (MProd) comprising: providing a composite material comprising a matrix of oxidised reductant (Ro), a product metal (MP) dispersed in the matrix of oxidised reductant (Ro), and one or more metal compounds (MPCR) of the product metal (MP) in one or more oxidation states dispersed in the matrix of oxidised reductant (Ro); and treating the composite material to at least partially remove the one or more metal compounds (MPCR) from the matrix of oxidised reductant (Ro) to form the metal-containing product (MProd).

Abstract: This method for recovering a valuable metal from a used LIB includes: a step of adding, to an electrode assembly taken out of a detoxified used LIB, metallic zinc in an excess amount relative to a mass of the electrode assembly; a step of heating a mixture of the electrode assembly and the metallic zinc to form a molten metal; a step of taking out the molten metal and separating the molten metal into an alloy metal and a slag; and a step of heating the alloy metal to volatilize zinc in the alloy metal, and thereby, recovering an alloy metal of a valuable metal.

Abstract: The present disclosure discloses an application of cuprous sulfide in a recovery of Au (III) from aqueous solutions, which relates to the fields of hydrometallurgy and precious metal recovery. The method of the present disclosure uses cuprous sulfide nanoparticles to recover Au (III) from aquesous solution, and undergoes gold adsorption under mechanical stirring. The method described in the present disclosure can efficiently recover Au (III) from aqueous solutions, has good recovery effects on Au (III) from acidic waste liquid, and has the advantages of energy conservation, environmental protection, and low cost.

Abstract: A device for preparing ultra-high purity zinc based on intelligently-controlled zone melting, including a slide platform connected with a screw through a servo control system to control movement of a heating-cooling device. A quartz tube is provided inside an induction heater to protect a melting zone. An infrared thermometer is connected to the heater, and configured to monitor temperature within the melting zone, and control power of the heater. A ring magnetic stirrer with non-contact circumferential rotation cooperates with coil to stir zinc melt. A water-cooling copper jacket is connected to two ends of the heater to cool a zinc bar, and its water inlet and outlet are connected with a water chiller. The infrared thermometer monitors temperature of the zinc bar and controls water flow of the cooling system. A lifting device is connected with a base cabinet to change inclined angle of the zinc bar.

Abstract: An apparatus for extracting lithium from a lithium-bearing material. A mixer is configured to receive and mix lithium-bearing material with gypsum, a sulfur-containing material, and a calcium-containing material and form a feed mixture having a moisture content of at least 20 wt %. A dryer is configured to dry the feed mixture and form a dried mixture having a moisture content of less than 20 wt %. A roaster is configured to receive and roast the dried mixture and form a roasted mixture including a water-soluble lithium compound. A leach tank is configured to form a lithium-containing leachate from the water-soluble lithium compound using an aqueous solution.

Abstract: A method for making a braking band (2) for a brake disc (1) for a disc brake, comprising the following steps: a) preparing a mold (10) having an inner cavity (11), which comprises a first portion (11a) of a shape corresponding to the braking band (2) to be made; b) preparing a band preform (20) comprising a central preform (200), an upper outer preform (201) and a lower outer preform (202), said central preform (200) being made of porous ceramic material comprising silicon carbide (SiC), said upper outer preform (201) and lower outer preform (202) being made of porous ceramic material comprising silicon carbide (SiC) and infiltrated with silicon (SiC+Si), wherein a carbon barrier layer (201a, 200a, 200b, 202a) made of carbon is interposed between the upper outer preform (201) and the central preform (200) and between the lower outer preform (202) and the central preform (200), said preforms (200, 201, 202) having the shape of the braking band (2) to be made; c) placing said band preform (20) inside the mold a

Abstract: An aluminum alloy material includes 1.0 wt % to 13.0 wt % of Si, 0.2 wt % to 1.4 wt % of Fe, 0.2 wt % to 0.8 wt % of Ni, and the remainder being Al and inevitable impurities. The aluminum alloy material can be 3D printed or die-casted to form an aluminum alloy object with a high thermal conductivity.

Abstract: An improved aluminum alloy for casting into a component, such as a vehicle component, is provided. The aluminum alloy preferably includes at least 80 weight percent (wt. %) aluminum; 9.5 to 11.5 wt. % silicon; 1.5 max wt. % zinc; 0.1 wt. % to 0.6 wt. % magnesium; 0.10 wt. % to 0.60 wt. % copper; 0.2. to 0.6 wt. % manganese; 0.2 wt. % to 0.6 wt. % iron; 0.01 wt. % to 0.07 wt. % strontium; and up to 0.15 wt. % titanium, based on the total weight of the aluminum alloy. This improved aluminum alloy can be formed by combining recycled aluminum or a recycle aluminum alloy with at least one additional element.

Abstract: The invention relates to improved sheets for decorative and formable parts made of an aluminium alloy which comprises, in wt. %, Mg: 0.8-1.3, Mn: 0.8-1.2, Cu: 0.05-0.30, Cr: 0.05-0.30, Fe: ?0.4, Si: ?0.3, Zn: ?0.25, Ti: ?0.15, impurities<0.05 each and <0.15 total, remainder aluminium. The method to make a sheet according to the invention comprises casting a slab with a composition according to the invention, homogenizing, hot and cold rolling the slab, annealing and tension leveling. The sheet according to the invention are particularly useful to make a coated decorative part preferably a decorative part for an automobile, or a household appliance or a medical device.

Abstract: The method includes slab-heating a steel slab to a temperature of higher than a ?-phase precipitation temperature and 1380° C. or lower, subjecting the steel slab to rough rolling including at least two passes of rolling at a predetermined temperature with an introduced sheet thickness true strain ?t of 0.50 or more and to finish rolling with a rolling finish temperature of 900° C. or higher to obtain a hot-rolled sheet, cooling the sheet for 1 second or longer at a cooling rate of 70° C./s or higher within 2 seconds after finish rolling, coiling the sheet at a coiling temperature of 600° C. or lower, performing hot-rolled sheet annealing for soaking at a predetermined soaking temperature, and then performing cold rolling, primary recrystallization annealing, and secondary recrystallization annealing.

Abstract: The present disclosure relates generally to alloys, and in particular, to iron alloys.

Abstract: The present invention relates to a steel material containing: 0.19 mass %?C?0.31 mass %; 0.010 mass %?V?0.180 mass %; Mn?1.50 mass %; 5.60 mass %?Cr?6.60 mass %; Cu+Ni?0.84 mass %; 0.60 mass %?Si?1.40 mass %; 0.60 mass %?Mo?2.00 mass %; 0.001 mass %?Al?0.080 mass %; and 0.003 mass %?N?0.040 mass %, with a balance being Fe and unavoidable impurities, in which the contents of Mn and Cr satisfy Mn/Cr>0.150.

Abstract: A martensitic stainless steel sheet for a brake disc rotor, characterized by containing predetermined components and having, in a mother phase, precipitates with a particle size of 2 ?m or less and a density of 0.01 to 20 pieces/?m2. The precipitates finely present in hot rolling and hot-rolled sheet annealing improves productivity in hardening, improves temper softening resistance of the steel sheet in use as a component, inhibits cracking in hot stamping, and inhibits reduction in high-temperature strength. Accordingly, the martensitic stainless steel sheet excellent in temper softening resistance, hardenability, formability, and high-temperature strength that is applicable to the disc rotor is provided.

Abstract: A hot-stamp formed body has a predetermined chemical composition and has a microstructure including, by area ratio, martensite: 90% to 100% and a remainder in the microstructure: 0% to 10%. The percentage of martensite having a GAIQ value of 40000 or less in all of the martensite is less than 5.0%, an average grain size of prior austenite grains is 6.0 ?m or less, and a standard deviation of grain sizes of the prior austenite grains is 2.6 ?m or less.

Abstract: An amorphous alloy soft magnetic powder contains a particle having a composition with a compositional formula (Fe1-xCrx) a (Si1-yBy)100-a-bCb expressed by an atomic ratio, in which 0<x?0.06, 0.3?y?0.7, 70.0?a?81.0, and 0<b?3.0, and when XAFS measurement is performed with an analysis depth set to a bulk, an obtained Fe—K absorption edge XANES spectrum has a first absorption edge structure having a peak A present in a range of 7113±1 eV and a first continuous band structure positioned at a higher energy side than the first absorption edge structure, and an intensity of the peak A at 7113 eV is 0.60 or more and 0.90 or less when an intensity of the first continuous band structure is 1.

Abstract: The present invention relates to a method of case hardening a Group IV metal or a Group IV metal alloy and to components hardened in the method. The method comprising the steps of: providing a workpiece of a Group IV metal or a Group IV metal alloy, the workpiece being in its final shape; nitriding the workpiece in a nitriding atmosphere comprising NHs as a nitriding species at a first temperature in the range of 450° C. to 750° C. for a nitriding duration of at least 16 hours to provide a hydrogen containing diffusion zone; removing hydrogen from the hydrogen containing diffusion zone at a second temperature of up to 750° C. and a partial pressure of H2 of up to 10?4 mbar over a hydrogen removal duration of at least 4 hours to provide a hydrogen depleted diffusion zone. The method and the component are useful for implants, in particular dental implants.

Abstract: Provided are an Al—Mg—Si based aluminum alloy forging excellent in mechanical properties in room temperature and hardly causing recrystallized grains and a production method thereof. An aluminum alloy forging consists of: Cu: 0.15 mass % to 1.0 mass %, Mg: 0.6 mass % to 1.15 mass %; Si: 0.95 mass % to 1.25 mass %; Mn: 0.4 mass % to 0.6 mass %; Fe: 0.2 mass % to 0.3 mass %; Cr: 0.11 mass % to 0.25 mass %; Ti: 0.012 mass % to 0.035 mass %; B: 0.0001 mass % to 0.03 mass %; Zn: 0.25 mass % or less; Zr: 0.05 mass % or less; and the balance being aluminum and inevitable impurities. The number of intermetallic compounds of Mg2Si with a minor axis of 0.5 ?m or more present in a visual field area of 1.5815 mm2 is 100 or less when a sectional structure of the aluminum alloy forging is observed at a magnification of 1,000 times.