Abstract: The present disclosure provides a reagent for detecting an expression level of a human histamine receptor HRH4 mRNA, a kit and a detection method. In the present disclosure, the reagent includes a specific primer and a probe for a human histamine receptor HRH4, the specific primer includes an HRH4-F and an HRH4-R, and the probe includes an H4-Probe; and the HRH4-F has a nucleotide sequence shown in SEQ ID NO. 1, the HRH4-R has a nucleotide sequence shown in SEQ ID NO. 2 and the H4-Probe has a nucleotide sequence shown in SEQ ID NO. 3. In the present disclosure, a kit for one-step detection and a detection method based on the reagent are prepared, and the expression level of the HRH4 mRNA can be one-step quantitatively detected with simple operation and short detection time.

Abstract: Improved solid supports and methods for analyzing target nucleotide sequences are provided herein. Certain improvements are directed to efficiently preparing nucleic acids that comprise nucleotide sequences identical to or substantially identical to one or more target nucleotide sequences, or complement thereof. The prepared nucleic acids include a reference sequence that facilitates sequence analysis. The solid supports and methods provided herein minimize the number of steps required by published sequence analysis methodologies, and thereby offer improved sequence analysis efficiency.

Abstract: Methods of monitoring the development of FNAIT using immune profiling is described.

Abstract: The present disclosure provides methods and systems for analyzing a biological sample from a subject, comprising using probes to identify and determine the location of different genetic sequences.

Abstract: The present disclosure generally relates to compositions and methods for predicting or diagnosing immune-related adverse events (irAE) before, during, or after immune checkpoint inhibitor (ICI) treatment in a subject with cancer. The method includes assessment of transcripts, autoantibody levels, cytokine levels, and immune cells. The irAE can be ICI-related myositis, ICI-related myocarditis or ICI-related myositis and myocarditis.

Abstract: The present disclosure provides methods and systems directed to methylation profiling for cell-free identification and/or monitoring of pregnancy-related states. A method for identifying or monitoring a presence or susceptibility of a pregnancy-related state of a subject may comprise assaying a cell-free biological sample derived from said subject to detect a set of biomarkers, and analyzing the set of biomarkers with a trained algorithm to determine the presence or susceptibility of the pregnancy-related state.

Abstract: Provided is a method capable of efficiently preparing a photoaged cell. Also provided is a novel cell marker that can be used for detection, quantification, or sorting out of a photoaged cell. The present disclosure includes a method for preparing a photoaged cell, including the step of: irradiating a cell with light having an emission peak within a wavelength range of 300 to 315 nm, and a method for detecting or quantifying a photoaged cell, including the step of: detecting or quantifying expression of at least one gene selected from the group consisting of GPR17, CD34, GABRR1, OR2AG2, CMKLR1, CDH19, CD93, AVPR2, CCR7, and OXGR1 in a cell.

Abstract: The present disclosure provides methods for determining whether a patient diagnosed with or at risk for metastatic castration-resistant prostate cancer will benefit from or is predicted to be responsive to treatment with a PARP inhibitor. These methods are based on detecting a co-deletion in BRCA2 and RB1 in a biological sample obtained from a prostate cancer patient. Kits for use in practicing the methods are also provided.

Abstract: Disclosed herein are methods, non-transitory computer readable media, systems, and kits for performing an intra-individual analysis for determining presence or absence of a health condition in an individual. Specifically, the intra-individual analysis involves combining sequence information from target nucleic acids with sequence information from reference nucleic acids obtained from the individual. The target nucleic acids include signatures that may be informative for determining presence or absence of the health condition and the reference nucleic acids include baseline biological signatures of the individual. By combining sequence information from the target nucleic acids and the reference nucleic acids, the resulting generated signal is more informative for determining presence or absence of the health condition in comparison to sequence information of the target nucleic acids alone.

Abstract: Disclosed herein are 2 novel long non-coding RNAs (lncRNAs), TROLL-2 and TROLL-3. It is shown herein that lncRNAs TROLL-2 and TROLL-3, as well as their effector WDR26, are suitable targets for cancer therapies and can be used to make prognostic determinations about a cancer and determine if immune checkpoint inhibitors should be used to treat a cancer.

Abstract: An oral cancer detection kit includes a reagent that detects methylation in promoter regions of a plurality of types of genes in DNA contained in a sample collected from the oral cavity of a subject. The plurality of types of genes includes KLLN, CASP8, CHFR, and GSTP1. Whether the subject has oral cancer is determined based on information on methylation in the promoter regions provided by the reagent.

Abstract: Methods and materials for identifying and treating mammals (e.g., humans) having multiple myeloma (MM) are provided herein. For example, this document provides methods and materials that can be used to identify and treat mammals having advanced stage MM, and/or having MVI that is resistant to treatment with immunomodulatory drugs (IMiDs) and/or proteasome inhibitors (PIs).

Abstract: Provided herein is a DNA analysis method comprising a procedure that affects a first nucleobase in the DNA differently from a second nucleobase in the DNA of the first subsample; partitioning a sample into at least a first subsample and a second subsample, wherein the first subsample comprises DNA (e.g., cell-free DNA) with a nucleobase modification in a different proportion than the second subsample; and DNA is sequenced to distinguish the first nucleobase from the second nucleobase. Also provided is a combination comprising first and second populations of captured DNA, wherein the first population comprises or was derived from DNA with a nucleobase modification in a different proportion than the second population, and wherein the first population comprises a form of a first nucleobase originally present in the DNA with altered base pairing specificity and a second nucleobase without altered base pairing specificity.

Abstract: The present application provides methods of treating a cancer in a subject who has one or more mutations in the LKB1 pathway.

Abstract: The present disclosure relates to a laboratory execution system that provides for automation of laboratory processes. A centralized data management system may be dynamically updated and used to facilitate management of components of the laboratory execution system, such as an automation system and an analytics results management system that may facilitate complex analytical functions, such as synthesizing raw test data. Potential workflows include the detection of specific molecules of interest.

Abstract: Provided herein are methods for the detection of EGFRvIII mutation in patient samples, e.g., in tumor tissue, cerebrospinal fluid, and plasma samples, using an optimized ddPCR assay. This methods can be used, e.g., for diagnosis, monitoring tumor progression, and assessing response to therapy.

Abstract: Aspects of the disclosure are directed to methods and systems for diagnosis, classification, and treatment of small cell lung cancer and high grade neuroendocrine carcinomas. Certain aspects comprise detection and analysis of tumor DNA methylation. Included in the disclosure are methods for identifying a subject as having a particular small cell lung cancer subtype based on DNA N methylation analysis, as well as methods and compositions for treatment of a subject based on subtype classification.

Abstract: The present invention concerns microRNAs as biomarkers for the in vitro diagnosis of glioma and/or for the in vitro discrimination of a glioma with a mutation in the IDH1 gene from a glioma with IDH1 wild-type gene.

Abstract: Assays and methods for detecting resistance to beta-lactam antibiotics including detection of multiple ?-lactamase family specific gene targets by polymerase chain reaction or microarray. One or more kits including primers and/or probes for identification of ?-lactamase genes selected from the group consisting of one or more of the following: MOX-like, FOX-like, ACC-like, ACT/MIR-like, CMY-2-like, DHA-like, CTX-M-14-like, CTX-M-15-like, VIM-like, NDM-like, IMP-like, KPC-like, and OXA-48-like, OXA-51-like, OXA-143-like, OXA-58-like, OXA-23-like, OXA-24/40-like, TEM-like, and SHV-like. A kit may also include one or more primers and/or probes for the identification a non-beta lactamase gene family which confers antibiotic resistance, such as the MCR-1 gene.

Abstract: This disclosure provides methods for detecting microbial cell-free nucleic acids for use in a wide variety of contexts. In some embodiments, this disclosure provides methods of monitoring a response to treatment. In some embodiments, this disclosure provides methods applicable for detecting bacterial infections (e.g., Bartonella spp. infections) and/or fungal infections. In some embodiments, the microbial cell-free nucleic acids are detected by metagenomic next-generation sequencing.

Abstract: The present disclosure relates to a monolayer graphene-based multiplex malaria diagnostic sensor. Specifically, a monolayer graphene-based sensor that is able to simultaneously detect the presence of different Plasmodium species, presence of drug-resistant Plasmodium species, and also the presence of a relevant polymorphism in a subject. The present disclosure also relates to a monolayer graphene-based sensor, method and kit for a rapid diagnosis of malaria using a non-invasive biological sample obtained from a subject, preferably in saliva or urine samples.

Abstract: The present invention provides a method for quantitatively detecting virus-derived RNA and/or DNA in an environmental sample or a fecal sample with high sensitivity, and a kit for quantitatively detecting virus-derived RNA and/or DNA in an environmental sample or a fecal sample with high sensitivity.

Abstract: Disclosed are methods and systems to detect a class of non-variola Orthopoxviruses, and, more particularly, monkeypox virus. In certain embodiments, the method may comprise obtaining a sample from the subject; and detecting a nucleic acid sequence specific to the non-variola Orthopoxvirus. In some embodiments, the method may further isolation of the non-variola Orthopoxvirus from a solid substrate such as a tissue swab and further purification of viral nucleic acid using silica beads. The sample may in some cases be self-collected by a subject. The disclosed methods and systems, including kits for self-collection, may be used for a high throughput assay allowing for screening of hundreds of samples per day.

Abstract: Provided herein are methods for identifying sequences of viruses. Also provided are compositions for carrying out the same. Compositions provided herein may comprise, consist essentially of, or consist of a plurality of primers. Furthermore, the disclosure provides kits comprising the compositions. Kits provided herein may also comprise oligonucleotides, probes, and solid supports.

Abstract: A multi-target nucleic acid detection kit for human papillomavirus (HPV) typing includes a recombinase polymerase amplification (RPA) primer set, an enzyme and a buffer system for Cas12a-crRNA, and a reporter molecule that displays a signal. In the detection method, a multi-channel microfluidic chip is used as a carrier, and RPA primers are designed for corresponding regions of different HPV subtypes to allow isothermal amplification. A crRNA set is designed for amplicons of different subtypes. The crRNA recognizes an HPV target in a sample, and then activates the CRISPR-Cas12a and cuts a reporter group to release a signal, thereby achieving accurate detection on HPV subtypes. The detection method shows a high sensitivity, a low cost, and easy operations, and is expected to be widely used in the screening of HPV infection.

Abstract: Disclosed are a method and a device for power allocation in a carbon dioxide incubator, and a carbon dioxide incubator. The method for power allocation in a carbon dioxide incubator provided with heating wires on multiple inner surfaces of the carbon dioxide incubator includes: according to a current temperature and a target temperature of a box body of the carbon dioxide incubator, determining a power index; in case where the current temperature is less than or equal to the target temperature, according to the power index, determining a heating strategy for each inner surface of the carbon dioxide incubator by looking up a table; and according to the heating strategy for each inner surface, adjusting at least one or more of the power and/or the start-stop time of the heating wires on each inner surface.

Abstract: The present invention describes the agricultural and/or forestry use of a mixture comprising: glycerol 5-90%, and glycerides of one or more organic acids 10-95% where the % are by weight with respect to the total mixture; wherein said mixture is used as a phytostimulating agent to promote the germination of seeds and/or as a fungicide/pesticide to protect seeds and/or crops against pathogenic microorganisms.

Abstract: Method for producing direct reduced metal material (106) in a continuous process, wherein hydrogen gas and inert gas is circulated in respective closed-loop first and second gas circuits via different respective gas connection stations (130,140120-122,150-151), comprising for individual mobile furnaces (101): a) charging metal material (106) into the furnace; b) moving and connecting the furnace to an inert gas connection station; c) providing heated inert gas to the furnace; d) disconnecting the furnace; e) moving and connecting the furnace to a hydrogen gas connection station; f) providing heated hydrogen gas to the furnace; g) disconnecting the furnace; h) moving and connecting the furnace to an inert gas connection station; i) providing cooled inert gas to the furnace; j) disconnecting the furnace; and k) discharging the metal material. The invention also relates to a system.

Abstract: A process for smelting high-speed steel by using an intermediate frequency furnace-LF furnace-VD includes: firstly, melting a steel material in an intermediate frequency furnace, smelting until the steel material is melted, adjusting chemical components, and then performing deoxidation and slagging; performing electric heating for steel tapping, sending the steel tapping into an LF furnace for smelting, heating to 1470-1510° C., and adjusting the chemical components; and finally, sending these components into a VD furnace for vacuum treatment, and performing steel tapping after the components are qualified. A slagging process in the intermediate frequency furnace is used, then the coarse adjustment of chemical components, the primary deoxidation, and the slagging are performed in the intermediate frequency furnace. After the molten steel is sent to the LF furnace, the slag adjustment can be performed. A content of oxygen in the molten steel can be controlled within 10 ppm after the VD vacuum treatment.

Abstract: A cold-workable mechanical structural steel may include: C: 0.30 to 0.45 mass %; Si: 0.10 to 0.40 mass %; Mn: 0.50 to 1.00 mass %; P: 0.050 mass % or less; S: 0.050 mass % or less; Cr: 0.80 to 1.30 mass %; Al: 0.01 to 0.10 mass %; and a balance of iron and inevitable impurity, the steel having an area percentage of pro-eutectoid ferrite of 10% or larger and 70% or smaller; containing at least one selected from the group consisting of bainite, martensite, and pearlite; and having a dislocation density of 3.5×1014 m?2 or larger.

Abstract: A steel pipe for high-pressure hydrogen, the steel pipe having a chemical composition containing, by mass %, C: 0.05% to 0.60%, Si: 0.001% to 2.0%, Mn: 0.01% to 5.0%, P: 0.030% or less, S: 0.010% or less, N: 0.010% or less, Al: 0.0001% to 1.00%, 0: 0.010% or less, H: 0.00010% or less, and a balance of Fe and incidental impurities and a metallic microstructure including, in terms of area fraction, 3% or less (including 0%) of retained austenite, in which the number of inclusions having an aspect ratio of 2.0 or more and a length of 10 ?m or more is 15/100 mm2 or less.

Abstract: Steel sheet able to reduce die damage at the time of cold pressing, that is, steel sheet having a predetermined chemical composition and steel microstructure which has on the sheet surface a plurality of step differences having height differences of more than 5.0 ?m at intervals of 2.0 mm or less.

Abstract: To provide a galvanized steel sheet having high strength; specifically, with a tensile strength of 1160 MPa or more and excellent resistance spot weldability. A chemical composition of a base steel sheet contains one or more of Ti, Nb, V, and Zr: 0.02% or more and 0.20% or less in total, and an amount of diffusible hydrogen in a zinc or zinc alloy coating layer is 0.40 mass ppm or less.

Abstract: The present disclosure provides a hydrometallurgical method for recovering lithium from a lithium-containing mineral through calcination and water leaching with a metal oxide without excessive use of chemicals, and a lithium aqueous solution recovered therefrom.

Abstract: Provided are a noble metal adsorbent, a method for recovering a noble metal, and a method for regenerating a noble metal adsorbent that can easily recover noble metal while high adsorption performance for noble metals is achieved. The noble metal adsorbent according to the present invention includes a metal sulfide. The metal sulfide is constituted of, for example, molybdenum disulfide particles. The method for recovering a noble metal according to the present invention includes adsorbing a noble metal onto the noble metal adsorbent, and thereafter heating and volatilizing the noble metal adsorbent in the presence of oxygen to recover the noble metal.

Abstract: Methods for leaching and extraction of precious metals.

Abstract: A method for the treatment of jarosite and other residues of the zinc and lead production industry, including the following steps: thermal treatment of the residues, at a temperature between 500° C. and 700° C., to obtain the evaporation of the imbibition water and the demolition of the jarosite molecule, resulting in the development of SO3 and in the removal of OH? groups to give additional water in the gaseous phase, and resulting in the obtainment, from the recondensation of these components, of an aqueous solution of diluted sulfuric acid, and with the simultaneous formation of a solid fraction composed of iron(III) oxide, zinc, lead, silver, copper sulphates and other minor elements; and then steps for acid leaching, a refining treatment; and purification to obtain solubilization of iron(III); separation of the solubilized iron(III) from the silica, which remains insolubilized; precipitation of iron(III) to obtain iron oxide pigment.

Abstract: The invention relates to metallurgy, and more particularly to producing titanium alloy-based materials with specific mechanical properties for the manufacture of fasteners for use in various fields of industry and preferably in the aerospace industry. The claimed material for the manufacture of high-strength fasteners is made from a titanium alloy containing alloying elements in the form of ?-stabilizers, #-stabilizers and neutral strengthening elements, the rest being titanium and unavoidable impurities. The size of a beta-subgrain in the structure of the material, which is subjected to solution annealing and aging, does not exceed 15 ?m.

Abstract: To provide an aluminum alloy in which natural aging is suppressed and a method for producing the same. This disclosure relates to an Al—Si-based aluminum alloy containing Si, Mg, Mn, Fe, and unavoidable impurities and Al—Si—Mg—Fe—Mn-based compounds, and further to a method for producing said Al—Si—based aluminum alloy by controlling the cooling process of a molten alloy during casting.

Abstract: The disclosure relates an Al—Mg series aluminum alloy sheet, a preparation method and use thereof. The preparation method includes: step S1, melting and casting the alloy raw materials in sequence to obtain an aluminum alloy ingot; step S2, subjecting the aluminum alloy ingot to a two-stage homogenization treatment so as to obtain a homogenized aluminum alloy ingot; and step S3, subjecting the homogenized aluminum alloy ingot to hot rolling, primary cold rolling, intermediate annealing, final cold rolling, and finished product annealing in sequence to obtain the aluminum alloy sheet.

Abstract: A medical device that is at least partially formed of a metal alloy that includes at least 15 awt. % rhenium, and a medical device that is partially or fully formed of such metal alloy.

Abstract: An example MPEA filler includes manganese at about 10 atomic percent to about 60 atomic percent, iron at about 0 atomic percent to about 35 atomic percent, cobalt at about 0 atomic percent to about 35 atomic percent, nickel at about 0 atomic percent to about 35 atomic percent, and copper at about 0 atomic percent to about 35 atomic percent.

Abstract: This grain-oriented electrical steel sheet includes a base steel sheet, a glass coating formed on the base steel sheet, and a tension-applied insulation coating formed on the glass coating, in the base steel sheet, a plurality of linear strains that extend continuously or intermittently in a direction intersecting with a rolling direction are present, and intervals in the rolling direction of the plurality of linear strains adjacent to each other are 10 mm or less, in a region where the strains are present, a closure domain is present, a length d of the closure domain in a sheet thickness direction from a surface of the base steel sheet is 30 to 60 ?m, a length w in the rolling direction is 200 ?m or less, and a ratio mid of a depth m from the surface of the base steel sheet where a compressive strain in the rolling direction present in the closure domain exhibits a maximum value to the length d is in a range of more than 0.30 and less than 0.90.

Abstract: Proposed is a method for producing a grain-oriented electrical steel sheet that comprises heating a steel material containing a certain composition and inhibitor-forming ingredients to a temperature in a range of 1300 to 1400° C.; performing hot rolling, hot-band annealing, and cold rolling on the material to obtain a cold-rolled sheet; performing decarburization annealing also serving as primary recrystallization annealing on the cold-rolled sheet; subsequently applying an annealing separator to a surface of the steel sheet; and performing finishing annealing followed by applying an insulation coating. In the method, rapid heating is conducted from 500 and 700° C. in a heating process of the decarburization annealing at a rate of 100 to 1000° C./s while gradual heating is conducted from 860 to 970° C. in a heating process of the finishing annealing at a heating rate of 0.5 to 4.0° C./hr for at least 10 hours.

Abstract: A clad steel plate having tensile strength (TS) of 780 MPa or more, excellent bendability, collision resistance, and LME resistance. The clad steel plate having a base metal and a cladding metal on front and back surfaces of the base metal, and the chemical composition and microstructure of the base metal and the cladding metal being appropriately controlled so that the average Vickers hardness (HVL) of the cladding metal is 260 or less, the average Vickers hardness (HVL) of the cladding metal divided by the average Vickers hardness (HVB) of the base metal is 0.80 or less, the boundary roughness between the base metal and the cladding metal is 50 ?m or less at the maximum height Ry, and the number of voids at the boundary between the base metal and the cladding metal is controlled to 20 or fewer per 10 mm length of the boundary.

Abstract: Disclosed is an iron-nickel alloy having the following composition in percent by weight: 36.5%?Ni?38.5% 0.50%?Mn?1.25% 0.001%?Cu?0.85% 0.040%?C?0.150% 0.10%?Si?0.35% the remainder being iron and unavoidable impurities resulting from the manufacturing.

Abstract: The chemical composition of a steel material according to the present embodiment consists of, in mass %, C: 0.50 to 0.80%, Si: 1.20 to 2.90%, Mn: 0.25 to 1.00%, Cr: 0.40 to 1.90%, V: 0.05 to 0.60%, P: 0.020% or less, S: 0.020% or less, N: 0.0100% or less, Mo: 0 to 0.50%, Nb: 0 to 0.050%, W: 0 to 0.60%, Ni: 0 to 0.50%, Co: 0 to 0.30%, B: 0 to 0.0050%, Cu: 0 to 0.050%, Al: 0 to 0.0050%, and Ti: 0 to 0.050%, with the balance being Fe and impurities. In the microstructure of the steel material, an area fraction of pearlite is 90% or more, and in ferrite in the pearlite, a volumetric number density of V-based precipitates having a maximum diameter of 2 to 20 nm is 3000 to 80000 pieces/?m3.

Abstract: A steel powder is provided. The steel powder has a composition of, in wt. %, C 0.05-2.0, Mn 14.0-30.0, Al 5.0-10.0, Cr 3.0-10.0, Si 0.1-2.0, Ti 0.05-0.5, and, as optionals, Ni 0.0-0.2, N 0.0-1.0, O 0.0-0.50, with a balance of Fe and unavoidable impurities. A method of producing the powder is also provided.

Abstract: Stainless steel foil with improved durability to repeated bending under the extreme bending conditions demanded of foldable displays and rollable displays. This stainless steel foil is flexible stainless steel foil comprising rolled stainless steel foil with a thickness of 0.1 mm or less, having a tensile strength of 1,800 MPa or more, and having a maximum height roughness of Rz of 0.35 ?m or less obtained from a roughness curve of the stainless steel foil surface as measured in the same direction as a tensile direction.

Abstract: The present invention relates to a ferritic free-cutting stainless steel material having a component composition contains: in terms of mass %, 10.0%?Cr?25.0%, 0.2%?Mn?2.0%, 0.30%?Al?2.50%, 0.02%?Si?0.60%, and 0.10%?S?0.45%, and further two or more selected from the group consisting of: 0.03%?Pb?0.40%, 0.03%?Bi?0.40%, and 0.01%?Te?0.10%, with a balance being Fe and unavoidable impurities. The component composition satisfies: 900([C]+[N])+170[Si]+12[Cr]+30[Mo]+10[Al]?300, and ([Cr]+[Mo]+1.5[Si]+4[Al])/([Ni]+0.5[Mn]+30[C]+30[N])?7. The ferritic free-cutting stainless steel material contains sulfides having a circle equivalent diameter of 1.5 ?m or more, and the sulfides have an average circle equivalent diameter of 3.0 to 15.0 ?m, an average aspect ratio of 2.5 or less, and an area ratio of 0.5 to 2.0%, and the maximum value of Vickers hardness of 170 HV or less.