Patents Issued in May 9, 2024
  • Publication number: 20240150682
    Abstract: A composition for solubilizing polystyrene and/or styrene copolymers, including a first component and a second component, in which the first component is selected from solvents having a flashpoint of at least 25° C., and the second component is soluble in the first component. The use of the composition as a cleaning composition for the removal of polystyrene and/or styrene copolymers from a surface and a method for the removal of polystyrene and/or styrene copolymers from a surface.
    Type: Application
    Filed: March 15, 2022
    Publication date: May 9, 2024
    Inventors: Halil AKTAS, Herman Franciscus GRONDMAN
  • Publication number: 20240150683
    Abstract: To provide a solvent composition that can suppress corrosion of a metal, a cleaning method using the solvent composition, and a method for producing an article with a coating film. A solvent composition comprising, as a first component, 1-chloro-2,3,3-trifluoropropene and, as a second component, at least one member selected from the group consisting of 1,2,3,3-tetrachloropropene, 1,2,3-trichloro-3-fluoropropene, 1,3,3-trichloro-2-fluoropropene, 1,3-dichloro-2,3-difluoropropene, 2-chloro-3,3-difluoropropene, 2,3,3-trifluoropropene, 1,3-dichloro-1,2,2-trifluoropropane, 1-chloro-1,2,2,3-tetrafluoropropane, 2-chloro-1,1,3,3-tetrafluoropropane, 2-chloro-1,1,2-trifluoropropane and chloroform, characterized in that the ratio of the total content of the second component to the sum of the content of 1-chloro-2,3,3-trifluoropropene and the total content of the second component, is from 0.0001 to 1.0 mass %.
    Type: Application
    Filed: January 12, 2024
    Publication date: May 9, 2024
    Applicant: AGC Inc.
    Inventor: Satoshi KAWAGUCHI
  • Publication number: 20240150684
    Abstract: The disclosure provides compositions and methods for making a colorized solution of an aqueous disinfectant that is both stable in bulk solution and will fade to clear within a predetermined period of time after being applied to a surface, for example as a spray or film. The compositions and methods described here allow an end user to visualize both the extent of coverage and the duration of contact of the disinfectant with the surface, thereby providing more efficient disinfection of the surface.
    Type: Application
    Filed: October 20, 2023
    Publication date: May 9, 2024
    Inventors: Jason Kang, Kevin Tyan, Katherine Jin
  • Publication number: 20240150685
    Abstract: The present invention relates to a product for machine dishwashing, comprising a capsule containing two or more compartments. The first compartment contains a solid, powdered first composition; and a second compartment contains liquid or gel second composition. The bleaching performance has been optimised, leading to better removal of tea stains. This has been achieved by an optimal combination of an alkali metal carbonate, a bleach component, sodium silicate and/or sodium disilicate, a bleach catalyst, and a maximum amount of tetraacetylethylenediamine.
    Type: Application
    Filed: March 10, 2022
    Publication date: May 9, 2024
    Applicant: Conopco Inc., d/b/a UNILEVER
    Inventors: Amanda Jane Adams, Robert John Carswell, Graham Corlett, Marina Mixtro Serrasqueiro
  • Publication number: 20240150686
    Abstract: A product and method for reducing chill haze in a fermented liquid is disclosed. The product may comprise attapulgite or sepiolite or mixture thereof. The product or the attapulgite or the sepiolite may have a particle size distribution having a d50 of 5-19 microns or 10-19 microns. The product or the attapulgite or the sepiolite may have: a pore volume of 0.5-1.9 mL/g or 0.7-1.5; and/or a porosity of 30-90% or 40-80% or 45-75%. The attapulgite or sepiolite or mixture thereof may comprise particles having an intrinsic pore size in the range of 6-50 nm or 10-50 nm or 15-35 nm or 20-30 nm. The product may have a Stabilizing Power of 40-100% at a loading of 20-200 g of the product per HL of fermented liquid.
    Type: Application
    Filed: November 9, 2022
    Publication date: May 9, 2024
    Applicant: Active Minerals International, LLC
    Inventors: Bo Wang, Dennis Parker
  • Publication number: 20240150687
    Abstract: Provided herein are genetically modified yeast cells that recombinantly expresses a gene encoding a mutant beta-lyase. Also provided are methods of producing fermented products and methods of producing ethanol.
    Type: Application
    Filed: October 16, 2020
    Publication date: May 9, 2024
    Applicant: Berkeley Brewing Science, Inc.
    Inventors: Jeremy Roop, Charles Denby, Rachel Li, Nicholas Harris
  • Publication number: 20240150688
    Abstract: An alcohol free or low alcohol fermented malt based beverage is disclosed. The malt based beverage has an alcohol content of not more than 1.0 vol. % preferably not more than 0.7 vol. % having an aroma profile close to the one of alcoholic lager beers. The beverage has 7.00-30.00 ppm ethyl acetate and 0.01-0.20 ppm ethyl butyrate. The beverage preferably has the esters 0.05-2.00 ppm isoamyl acetate; 0.01-0.10 ppm ethyl butyrate; and 0.01-0.05 ppm ethyl hexanoate. The beverage preferably has the higher alcohol 5.00-30.00 ppm (iso-)amyl alcohol. The (iso)amyl alcohol is defined as the sum of 3-methyl butanol and 2-methyl butanol.
    Type: Application
    Filed: November 16, 2020
    Publication date: May 9, 2024
    Applicant: Anheuser-Busch InBev S.A.
    Inventor: Bart Vanderhaegen
  • Publication number: 20240150689
    Abstract: The present disclosure provides methods for treating one or more grains in an ethanol plant or alcohol brewery, methods of drying, cooling, disease control of dried distillers grains with soluble (DDG), barley, or brewer's spent grain (BSG), and a system for treating one or more grains in an ethanol plant or alcohol brewery by these disclosed methods.
    Type: Application
    Filed: February 17, 2023
    Publication date: May 9, 2024
    Inventor: Steffen Mueller
  • Publication number: 20240150690
    Abstract: A method for producing tissue constructs formed of one or more materials, dispensed or applied in patterned layers on two or more platforms, build plates or print surfaces by two or more stations, performing dispensing, mechanical modification, chemical modification, biological modification, or inspection operations. The platforms are positioned relative to the stations and station operations are directed by signals from a controller causing station operations on platforms to be performed simultaneously, for each patterned layer until all layers have been applied in sequence.
    Type: Application
    Filed: November 7, 2022
    Publication date: May 9, 2024
    Inventor: Ralph L. Stirling
  • Publication number: 20240150691
    Abstract: There is provided a bile duct chip that is a bile duct chip (200) including a membrane (210) through which substances are movable from one surface to the other surface, a first substrate (221) having a recessed part that forms a first flow path (220), and a second substrate (231) having a recessed part that forms a second flow path (230), in which the first substrate (221), the membrane (210), the second substrate (231) are stacked in this order, the recessed part of the first substrate (221) has an opening portion facing the one surface of the membrane (210), the recessed part of the second substrate has an opening portion facing the other surface of the membrane (210), the one surface of the membrane (210) forms a part of the first flow path (220), the other surface of the membrane (210) forms a part of the second flow path (230), the first flow path (220) and the second flow path (230) communicate with each other through the membrane (210), and bile duct epithelial cells (222) are disposed on a surface of
    Type: Application
    Filed: March 9, 2022
    Publication date: May 9, 2024
    Inventors: Kazuo TAKAYAMA, Yusuke TORISAWA, Kenji OSAFUNE, Maki KOTAKA, Sayaka DEGUCHI, Hiroyuki MIZUGUCHI
  • Publication number: 20240150692
    Abstract: In accordance with various embodiments of the disclosed subject matter, a system, device and platform for culturing and maintaining tissue representative cellular models combined with active fluidics mimicking body circulation. In combination, multiple chamber devices enable modeling of multi-organ communication, representative of in vivo conditions.
    Type: Application
    Filed: September 28, 2023
    Publication date: May 9, 2024
    Inventors: Saber M. Hussain, Richard Agans, Mark T. Nelson, Richard L. Salisbury
  • Publication number: 20240150693
    Abstract: The invention of the present application provides a sterile container for cell culture that is sufficiently sterilized but sufficiently retains a low cell adsorption property and that easily forms cell aggregates by cell culture. The sterile container for cell culture having a low cell adsorption property of the invention of the present application includes a cell accommodation part which is made of a resin material and is capable of accommodating cells and a liquid culture medium to perform cell culture, in which a coating layer made of a water-soluble resin having a hydrophilic functional group at a side chain is formed on an inner surface of the cell accommodation part, cross-linking is formed in the water-soluble resin itself and between the water-soluble resin and the inner surface of the cell accommodation part, and the container is subjected to a radiation sterilization treatment with an absorbed radiation dose of 20 kGy or more and 45 kGy or less.
    Type: Application
    Filed: March 8, 2022
    Publication date: May 9, 2024
    Inventor: Kohta Igarashi
  • Publication number: 20240150694
    Abstract: A plate for biological cell processing operation has a plate body and a lid. The plate body has an upper surface portion, a base portion, an annular wall portion extending above the base portion, a recess inside the annular wall portion, cell processing solution in the recess, and a sealing sheet sealing the recess. The lid covers the annular wall portion and recess of the plate and is placed over the base portion. The sealing sheet has a sheet body portion sealing the recess and a gripping portion for peeling. In the lid attached state, the gripping portion for peeling passes between the annular wall portion and the lid and is close to an upper surface portion of the base portion, while in the lid unattached state, the gripping portion for peeling rises up and away from the upper surface portion.
    Type: Application
    Filed: January 18, 2024
    Publication date: May 9, 2024
    Inventor: Futoshi INOUE
  • Publication number: 20240150695
    Abstract: A method for producing the cell culture vessel having the culture surface of cells includes: using a primary mold including a projecting portion to form an uneven pattern structure on a surface of a vessel base material and a secondary mold including a projecting portion to flatten a part of the surface of the vessel base material; pressing the primary mold against the vessel base material to form the uneven pattern structure on the vessel base material; pressing the secondary mold against a thin portion formed on the vessel base material to modify the thin portion to a flattened portion; and configuring the surface of the vessel base material including the uneven pattern structure formed with at least a part of the modified flattened portion as the culture surface to form the cell culture vessel.
    Type: Application
    Filed: January 12, 2024
    Publication date: May 9, 2024
    Inventors: Osamu KOSEKI, Satoshi TANAKA, Takahiko TOTANI, Takaharu NISHIYAMA
  • Publication number: 20240150696
    Abstract: This disclosure describes hardware for microfluidic chips and an associated platform for facilitating operation of one or more microfluidic chips. The microfluidic chips described herein are designed for supporting multiple different tissue types, including kidney tissue, liver tissue, adipose cells, and so forth. Chip geometry facilities fluid flow through one or more channels of the chip with a particular flow rate. For example, shear forces are reduced where needed to ensure proper flow rate of fluid in the channels. The chamber geometry and the geometry of the channels ensures that a desired amount of oxygen is delivered to sample cells or tissues in a controlled manner.
    Type: Application
    Filed: November 7, 2023
    Publication date: May 9, 2024
    Inventors: Shiny Amala Priya Rajan, Jacob Dylan Freake, Corbin Munn, Murat Cirit
  • Publication number: 20240150697
    Abstract: The present invention relates to a reconfigurable bioprocessing system (80) operable to perform a predetermined set of bioprocessing operations therein. The reconfigurable bioprocessing system (80) comprises a base unit (60) comprising a plurality of valve actuators (102). At least one of the plurality of valve actuators (102) is operable to releasably engage with at least part of a pinch valve cassette (103, 203) to control fluid flow through the pinch valve cassette (103, 203). At least part of the pinch valve cassette (103, 203) is removably attachable to the base unit (60) and the pinch valve cassette (103, 203) is provided adjacent to said plurality of valve actuators (102).
    Type: Application
    Filed: April 25, 2022
    Publication date: May 9, 2024
    Inventors: Klaus Gebauer, Tim Francois, Andreas Lundin, Bjorn Markus Olovsson, Kerstin Erickson, Tomas Dalmo
  • Publication number: 20240150698
    Abstract: A cell culture device includes a substrate, a wire disposed on a main surface of the substrate, an insulating film disposed on the main surface of the substrate and having a part that overlaps the wire, and the electrodes disposed on the insulating film and having a part that overlaps the wire. The insulating film has a contact hole at a position overlapping the wire and the electrode. A plurality of grooves are formed on at least the surface of a part of the electrode that overlaps the contact hole.
    Type: Application
    Filed: October 25, 2023
    Publication date: May 9, 2024
    Inventors: Chihiro TACHINO, Tomoko TERANISHI, Satoshi IHIDA, Takeshi HARA
  • Publication number: 20240150699
    Abstract: An electroporation system including one or more of a pipette, a pipette tip, a pipette docking assembly, and a pulse generator. The pipette docking assembly includes a pipette station, a pipette station guard, and a reservoir (e.g., a buffer tube). A method for transfecting a cell with a payload including providing an electroporation system, providing the cell, providing the payload, introducing the cell and the payload into a pipette tip, and electroporating the cell within the pipette tip by operating the electroporation system.
    Type: Application
    Filed: September 15, 2023
    Publication date: May 9, 2024
    Inventors: Han WEI, Chee Wai CHAN, Wui Khen LIAW, Shan Hua DONG, See Chen GOH, Huei Steven YEO, Harmon Cosme SICAT, JR., Mio Xiu Lu LING, Josh M. MEAD, Mikko MAKINEN, Beng Heng LIM, Kuan Moon BOO, Justina Linkai BONG, Chye Sin NG, Wee Liam LIM, Li Yang LIM, Way Xuang LEE
  • Publication number: 20240150700
    Abstract: The invention relates to a system (10) for determining a force applied to a cell or tissue culture (14) arranged in a culture chamber (12), comprising an elastic element (30) mounted in or suitable for mounting in said culture chamber (12), said elastic element (30) being adapted to be coupled with said cell or tissue culture (14) such that a force applied to said cell or tissue culture (14) leads to a deflection of said elastic element (30) against a restoring force thereof, and said elastic element comprising a magnetic element (34) arranged such as to be moved upon deflection of said elastic element (30), a magnetic field sensor (42) mounted or suitable for mounting outside said culture chamber (12), said magnetic field sensor (42), when mounted outside said culture chamber (12), being adapted to detect a change of magnetic field attributable to a corresponding movement of said magnetic element (34) upon deflection of said elastic element (30).
    Type: Application
    Filed: June 22, 2023
    Publication date: May 9, 2024
    Inventor: Andreas Dendorfer
  • Publication number: 20240150701
    Abstract: The present invention relates to a production system (10), in particular for producing biological-pharmaceutical products (20), and/or a method for producing a product (20), in particular a biological-pharmaceutical product (20).
    Type: Application
    Filed: January 17, 2024
    Publication date: May 9, 2024
    Applicant: KyooBe Tech GmbH
    Inventor: Mario BOTT
  • Publication number: 20240150702
    Abstract: The present invention relates to a production system (10), in particular for producing biological-pharmaceutical products (20), and/or a method for producing a product (20), in particular a biological-pharmaceutical product (20).
    Type: Application
    Filed: January 17, 2024
    Publication date: May 9, 2024
    Applicant: KyooBe Tech GmbH
    Inventor: Mario BOTT
  • Publication number: 20240150703
    Abstract: The present disclosure provides systems and methods for detecting the presence of colonies on agar plates. The systems and methods are suitably useful for evaluating the presence of a contaminant (e.g., bacterial, fungal, etc.) in a sample, such as a raw material or component of a pharmaceutical composition, by analyzing whether the samples produce colonies on an agar plate. The systems utilize a lighting arrangement to increase the accuracy of the analysis, and in embodiments, include the use of artificial intelligence as part of an automated process.
    Type: Application
    Filed: October 16, 2023
    Publication date: May 9, 2024
    Inventors: Archibald DELORME, Zichao BIAN
  • Publication number: 20240150704
    Abstract: Biological cells, which are stored on carriers in a storage module, are cultivated in a process module. Each cell line is assigned an application containing a treatment specification, which contains one or more workflows that are performed in the process module at successive time intervals, each with a respective start time. Each workflow contains a work step or a plurality of work steps to be performed in succession. The control device controls the parallel execution of a plurality of applications and starts the one or more workflows of the different applications one after another in the same process module. In order to economically perform the workflows successively in a single process module, each workflow is assigned an effective function dependent on the start time, and the sequence and the start times of the workflows of different applications are determined by minimizing or maximizing the sum of the effective functions.
    Type: Application
    Filed: March 14, 2022
    Publication date: May 9, 2024
    Inventors: Patrick Clemente GILLIGAN, Kenneth B. K. TEO, John Edward CONNAH, David Anthony BULLINARIA
  • Publication number: 20240150705
    Abstract: Vacuum infusion of plant extracts in live probiotic cells significantly enhances the viability of probiotics during in vitro gastrointestinal digestion with the retention of their metabolic and antagonistic activities. In-vivo results validate the significantly enhanced persistence and mucoadhesion of probiotic cells with infused plant extracts.
    Type: Application
    Filed: December 18, 2023
    Publication date: May 9, 2024
    Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
    Inventors: Nitin Nitin, Rewa Rai, Marluci Palazzolli da Silva Padilha
  • Publication number: 20240150706
    Abstract: The present invention relates to microencapsulated microbial cultures with high storage stability and methods for producing these. In particular, the present invention relates to microbial cultures formulated at high ratios of encapsulation matrix material to core material.
    Type: Application
    Filed: February 10, 2022
    Publication date: May 9, 2024
    Applicant: Chr. Hansen A/S
    Inventors: Deepak Jain, Surender Kumar Dhayal
  • Publication number: 20240150707
    Abstract: The present disclosure relates to a strain of Lactobacillus gasseri LM1065 (KCCM13018P) and a composition of relieving premenstrual syndrome containing the strain. A strain of Lactobacillus gasseri LM1065 (KCCM13018P) according to an embodiment of the present disclosure can relieve premenstrual syndrome by reducing NO in blood and TL-6 gene expression and increasing the production ratio of prostaglandin E1/prostaglandin E2 in blood and DGLA levels. Therefore, the strain can be applied to food compositions, health functional food compositions, pharmaceutical compositions, and the like.
    Type: Application
    Filed: January 15, 2024
    Publication date: May 9, 2024
    Inventors: Minn SOHN, Tae Rahk KIM
  • Publication number: 20240150708
    Abstract: A cell culture method includes performing adherent culture of cells on a coat layer layered on a surface of a cell culture container by placing a culture medium and the cells in the cell culture container. The coat layer includes a layer (i) that includes a layer (i-1) including gelatin or casein and a layer (i-2) that includes a polycationic material and a layer (ii) that is layered on the layer (i) and includes a cell adhesion factor. The layer (ii) is disposed at a position at which the layer (ii) comes into contact with the cells, or the coat layer includes the layer (i) and the layer (i) is disposed at a position at which the layer (i) comes into contact with the cells, and the culture medium includes the cell adhesion factor.
    Type: Application
    Filed: January 12, 2024
    Publication date: May 9, 2024
    Applicant: Ricoh Company, Ltd.
    Inventors: Momoko Shionoiri, Tomoyuki Aratani, Rie Ijichi, Tatsuya Sameshima, Shinnosuke Koshizuka, Tomofumi Kitazawa, Tomoaki Nakayama, Yuki Kaga
  • Publication number: 20240150709
    Abstract: Parasympathetic neurons (parasymN) are important for unconscious body responses, including rest-and-digest and calming the body. Disclosed herein is a chemically defined differentiation protocol that generates parasympathetic neurons from stem cells. The protocol yields high efficiency and purity cultures that are electrically active and respond to specific stimuli. Their molecular characteristics and maturation stage are described and evidence for their use as a model for studying parasymN function and dysfunction. Cell populations and compositions formed from the resulting cells, as well as methods of their use for disease treatment, drug screening, and modeling of human disorders affecting parasymN are also provided.
    Type: Application
    Filed: November 6, 2023
    Publication date: May 9, 2024
    Inventors: Nadja Zeltner, Hsueh Fu Wu
  • Publication number: 20240150710
    Abstract: Compositions and methods are provided for generation of assembled three-dimensional organoids with defined numbers and ratios of mature neurons and mature glia. Organoids can be assembled from mature neurons and mature glia derived from induced pluripotent stem cells having at least one genetic mutation associated with a neurological disorder, a neurodevelopmental disorder, or a neurodegenerative disease. Such organoids can be used in disease modeling and drug screening. In particular, assembled three-dimensional organoids are provided that model granulin (GRN) loss of function in neurons and astrocytes, which display many of the pathological features of neuronal ceroid lipofusis and frontotemporal dementia.
    Type: Application
    Filed: February 25, 2022
    Publication date: May 9, 2024
    Inventors: Erik M. Ullian, Mark Koontz, Robert Krencik
  • Publication number: 20240150711
    Abstract: The disclosure provides methods of reprogramming polyclonal T cells to maintain long-term persistence. The disclosure further provides methods of treatment, such as adoptive T cell transfer therapies, that harness Tscms for development of tumor-reactive T cells. In some embodiments, the disclosure provides methods and compositions for positive modulation of the Tscm-producing phenotype, e.g., positive modulation of TCF7 expression. Positive modulation of TCF7 expression allows for maintenance of an increased T number of stem-like T cells capable of both self-renewal and generation of differentiated, cytolytic progeny.
    Type: Application
    Filed: March 1, 2022
    Publication date: May 9, 2024
    Applicants: Dana-Farber Cancer Institute, Inc., The General Hospital Corporation
    Inventors: Marc Schwartz, Nir Hacohen
  • Publication number: 20240150712
    Abstract: The invention is directed to a method for activating CAR cells having at least one chimeric antigen binding receptor in a sample by incubating the sample with at least one substrate provided on its surface with at least one anti-CAR idiotype antibody and/or at least one antigen selected from the group consisting of CD19, CD20, CD22, BCMA, CD33, MSLN, CD123, HER2, GD2, EGFR, PSMA, MUC1, CD318, TSPAN8, CD66c, CEA, CLA, CD276, FolR1, CLEC12A, CLL-1 and CD371, and with at least one costimulatory molecule selected from the group consisting of CD28, CD2, CD6, CD26, CD53 and LFA-1 characterized in that the sample is incubated with the at least substrate in suspension thereby activating the CAR T cells to express markers selected from the group consisting of mRNA, effector molecules or cell surface activation markers.
    Type: Application
    Filed: March 14, 2022
    Publication date: May 9, 2024
    Applicant: Miltenyi Biotec B.V. & Co KG
    Inventors: Meike ROSKAMP, Cesar EVARISTO, Christian LANGER, Alexander DUNDUA, Anne RICHTER, Jonathan FAUERBACH
  • Publication number: 20240150713
    Abstract: The present invention provides a method for producing CAR T-cells enriched with stem cell-like memory T-cells (Tscm), comprised of: (1) providing PBMCs; (2) isolating of T cells from PBMCs using magnetic beads coated with T-cell specific antibodies; (3) positively selecting T cells by culturing them in the form of T cell-magnetic bead complexes; (4) adding CAR-expressing lentivirus to the activated human T cell-magnetic bead complexes, for transduction of human T cells into CAR-T cells on the human T cell-magnetic bead complexes to obtain human CAR-T cell-magnetic bead complexes; (5) obtaining human CAR-T cells by dissociating the human CAR-T cells from the human CAR-T cell-magnetic bead complexes; and (6) further culturing CAR-T cells to obtain CAR-T cells enriched with Tscm. The present invention expands T cells in a short period of time to obtain CAR-T cells enriched with Tscm, which is suitable for CAR-T immunotherapy.
    Type: Application
    Filed: October 13, 2023
    Publication date: May 9, 2024
    Inventors: Chien-Ting LIN, Chen-Lung LIN, Yi-Hui LAI, Xuan-Hui LIN, Tzu-Hsun TUNG
  • Publication number: 20240150714
    Abstract: There is provided a device, system and method for recovering magnetically tagged target cells from a fluid collection of cells. The device comprises a magnet plate having an array of micro-magnets and a modular chip releasably coupled to the magnet plate. The modular chip has a flow chamber and a plurality of flow rate-reducing structures. The magnetic field produced by the array of micro-magnets cooperates with the flow rate-reducing structures to define a capture zone in the vicinity of each structure. The magnetic field is sufficient to overcome drag force on the target cells to promote capture of the target cells in the capture zone. Separation of the modular chip from the magnet plate allows for recovery of the captured target cells from the modular chip.
    Type: Application
    Filed: February 25, 2022
    Publication date: May 9, 2024
    Inventors: Shana Olwyn KELLEY, Zongjie WANG, Sharif AHMED
  • Publication number: 20240150715
    Abstract: A formulation for a culture medium that is specifically designed to supplement a culture of differentiating hematopoietic stem cells (HSCs) with the factors necessary for high density manufacture of red blood cells (RBCs) while only requiring partial medium exchanges on a periodic basis. Further, the present disclosure identifies a factor, iron (III) citrate, also referred to as ferric citrate, that can supplant more commonly utilized sources of biological iron in culture medium for a more stable, more cost-effective medium formulation for superior vertical scalability.
    Type: Application
    Filed: November 8, 2023
    Publication date: May 9, 2024
    Applicant: Sciperio, Inc
    Inventors: Trevor Sasserath, Janice M. Moser, Kelly Haupfear, Casey W. Perkowski, Evan McDowell, Kenneth H. Church
  • Publication number: 20240150716
    Abstract: The present invention relates to an in vitro or ex vivo method for inducing a phenotypic and/or functional change in a population of mononuclear phagocytes isolated from biological samples. The method includes the incubation of the population in a culture medium which includes factors released from tumor cultures or explants. The incubation takes place under hypoxic conditions and the incubation induces a phenotypic and/or functional change in the mononuclear phagocytes of the population. The macrophages thus obtained assume a unique regenerative phenotype not present in other polarized phenotypes, including M2 macrophages. In addition, the invention relates to the use of the bioreactor thus produced for the regeneration of tissues, including neural tissue.
    Type: Application
    Filed: March 18, 2022
    Publication date: May 9, 2024
    Applicants: HEMERA S.R.L., HUMANITAS MIRASOLE S.P.A.
    Inventors: Ilaria DECIMO, Francesco BIFARI, Sissi DOLCI, Guido Francesco FUMAGALLI, Massimo LOCATI
  • Publication number: 20240150717
    Abstract: Embodiments herein include methods, kits, and compositions for manufacturing extracellular matrix (ECM). In some embodiments, the methods comprise differentiating fibroblasts into induced pluripotent stem cells, expanding the induced pluripotent stem cells, and differentiating the induced pluripotent stem cells into fibroblasts. The fibroblasts can produced mature ECM, which can be isolated and used for medical and/or cosmetic products and procedures.
    Type: Application
    Filed: June 14, 2023
    Publication date: May 9, 2024
    Inventor: Francis Christopher ZEIGLER
  • Publication number: 20240150718
    Abstract: It is provided a method for gene repair in primary human muscle stem cells (satellite cells) in vitro comprising the following steps: providing a sample of an isolated muscle-fiber containing tissue sample collected from at least one patient with a monogenic muscle disease, wherein the monogenic muscle disease is caused by at least one mutation in at least one gene encoding for at least one muscle protein; isolating and cultivating primary stem cells from said muscle-fiber containing tissue sample, and correcting the at least one mutation in the at least one gene encoding for at least one muscle protein in the cultivated primary stem cells by targeted modification of the at least one mutation by gene editing using CRISPR/Cas-based tools.
    Type: Application
    Filed: January 31, 2022
    Publication date: May 9, 2024
    Applicants: CHARITÉ - UNIVERSITAETSMEDIZIN BERLIN, MAX-DELBRÜCK-CENTRUM FÜR MOLEKULARE MEDIZIN
    Inventors: Simone SPULER, Helena ESCOBAR, Christian STADELMANN
  • Publication number: 20240150719
    Abstract: Apparatus and methods for culturing cells in a cell culture medium including magnetic beads in a fluid. A variable magnetic field can be applied to the magnetic beads to create shear forces on cells on the surface of the beads. In certain embodiments, a rotational force can also be applied to the magnetic beads and the magnetic force can counteract the rotational force.
    Type: Application
    Filed: February 21, 2022
    Publication date: May 9, 2024
    Inventors: Brijesh S. GILL, Charles S. COX, Jr.
  • Publication number: 20240150720
    Abstract: The present invention relates to 3D cell cultures, which contains hepatic cells and are infected by a pathogen, methods for preparing such cell cultures and uses thereof.
    Type: Application
    Filed: January 16, 2024
    Publication date: May 9, 2024
    Applicant: Merck Patent GmbH
    Inventors: Thomas SPANGENBERG, Beatrice Greco, Paula Maria Marques Leal Sanches Alves, Manuel José Teixeira Carrondo, Ana Catarina Mauricio Brito Ataíde, Sofia Raquel Paulo Rebelo, Francisca Maria De Andrade Terras Arez, Daniel Filipe Mestre Simão, Rui Miguel Prudêncio Pignatelli, Diana Marisa Pinto Freire Fontinha, Marta Monteiro Maia Machado
  • Publication number: 20240150721
    Abstract: The invention relates to the use of a laminin (LN) as a matrix for hepatic differentiation. The invention also relates to a method for inducing hepatic differentiation comprising the steps of: (i) providing a population of human pluripotent cells, (ii) culturing the population on a support coated with a laminin in a endoderm induction medium to produce a population of human DE cells, (iii) culturing said population of human DE cells on a support coated with a laminin in a hepatic induction medium to produce a population of human hepatoblasts-like cells, and (iv) optionally culturing said population of human hepatoblasts-like cells on a support coated with a laminin in a hepatic maturation medium to produce a population of human hepatocyte-like cells. The invention further relates to a population of human hepatoblasts-like cells or human fetal hepatocyte-like cells obtained by the method of the invention.
    Type: Application
    Filed: November 17, 2023
    Publication date: May 9, 2024
    Applicants: INSERM (Institut National de la Santé et de la Recherche Médicale), Université de Nantes
    Inventors: Tuan Huy Nguyen, Angélique Fourrier
  • Publication number: 20240150722
    Abstract: Described herein are human transgenic beta cells expressing fugetactic levels of CXCL12 to a subject in need thereof. Also described herein are beta cells comprising a transgene comprising a nucleic acid sequence encoding CXCL12.
    Type: Application
    Filed: July 25, 2023
    Publication date: May 9, 2024
    Inventors: Gerald F. Swiss, David Kiewlich
  • Publication number: 20240150723
    Abstract: In some instances, the application provides methods of culturing host cells comprising a gene encoding a recombinant N protein in a cell culture medium, wherein the cell culture medium comprises: (i) iron at a concentration of less than 1200 ?M; and (ii) citrate at a concentration of less than 2400 ?M. In some instances, the methods comprise culturing host cells expressing a recombinant protein in a cell culture medium at a first temperature; and decreasing the first temperature to a second temperature. In some instances, the methods comprise purifying the recombinant protein by a carbon depth filtration.
    Type: Application
    Filed: March 22, 2022
    Publication date: May 9, 2024
    Applicant: Biogen MA Inc.
    Inventors: Taylor Forte, Christoper Kwiatkowski, Greg Evangelist, Taylor Roland, Krishnakumar Malu
  • Publication number: 20240150724
    Abstract: The present disclosure generally relates, in certain aspects, to cultivated meat and other cultivated animal-derived products. In some embodiments, the whole blood of a non-human animal is separated into various components (e.g., concentrated red blood cells, blood plasma, etc.). Some embodiments are generally directed toward incorporating coloring or “redness” into a product. For example, colorants may be added to a cultivated meat product to improve its color. The colorant may comprise a lysate of non-human red blood cells, e.g., containing hemoglobin. In some embodiments, the lysate may be obtained from blood withdrawn from living animal donors. Other embodiments yet are generally directed toward the composition and method of use of the lysate, cultivated meat products, kits involving these, or the like.
    Type: Application
    Filed: March 9, 2022
    Publication date: May 9, 2024
    Applicant: Terasaki Institute for Biomedical Innovation
    Inventors: Seyedvahid Hosseini, Alireza Khademhosseini
  • Publication number: 20240150725
    Abstract: The present disclosure relates to a novel bacteriophage having ability to specifically kill Clostridium perfringens and an antibacterial composition comprising the same. The novel bacteriophage CJ_CP_20-25 has the effect of specifically killing Clostridium perfringens, exhibits excellent acid resistance and heat resistance, and can thus be widely used in antibiotics, feed additives, drinking water additives, feed, drinking water, disinfectants, or cleaning agents for the prevention or treatment of infectious diseases caused by Clostridium perfringens.
    Type: Application
    Filed: June 15, 2021
    Publication date: May 9, 2024
    Applicant: CJ CheilJedang Corporation
    Inventors: Jong Soo JEON, Ji Eun KIM, Jun Ok MOON, Jong Pyo CHAE, Yu Jin KIM, Seung Eun LEE
  • Publication number: 20240150726
    Abstract: A production method of recombinant adeno-associated virus particles (rAAV virions) having less empty viral contamination is disclosed. The production method includes: (a) culturing mammalian cells containing an introduced AAV vector in a medium to release rAAV virions into the medium; (b) recovering a culture supernatant from the medium; and (c) purifying the rAAV virions from the recovered culture supernatant by affinity column chromatography using a material having an affinity for an AAV capsid protein as a stationary phase and anion column chromatography.
    Type: Application
    Filed: March 8, 2022
    Publication date: May 9, 2024
    Applicant: JCR PHARMACEUTICALS CO., LTD.
    Inventors: Kota Ohtsuki, Tomoki Hirashima
  • Publication number: 20240150727
    Abstract: The present invention provides a new species of tobamovirus and its use to identify plants comprising resistance against the virus.
    Type: Application
    Filed: June 27, 2023
    Publication date: May 9, 2024
    Inventors: Bart Peter Johan GERAATS, Marco Antonio MAMMELLA, Massimo TURINA, Marina CIUFFO
  • Publication number: 20240150728
    Abstract: The invention relates to a method of increasing the yield of virus, virus particles, or viral vectors from host cells in a bioreactor. The invention provides a reproducible and robust method and system of determining and controlling the optimal time of infection of host cells using a correlation of process air parameters including Air flow, O2 flow, and respective trends thereof resulting in increased virus yield.
    Type: Application
    Filed: October 12, 2023
    Publication date: May 9, 2024
    Inventors: Perry NEWTON, Dalton BERRIE, Tyler GROW, Sheldon DOXILLY, Christopher J. MONTOYA, Sara Jane TERPENING, Eric VELA
  • Publication number: 20240150729
    Abstract: The instant invention discloses a closed-system, serum-free, microcarrier-based up-stream process to produce dengue virus. The process comprises a closed-system cell expansion comprising cell passages taking place in closed-system containers. These cell expansions represent a closed process, with replacement media, a cell-detachment agent, and quench medium added though sterile, weldable tubing (a closed-system environment) to eliminate all open aseptic processing following the initial vial thaw of the adherent cells. The viral production takes place in a closed-system bioreactor in adherent cell culture grown on microcarriers providing sufficient cell mass to support the production of dengue virus.
    Type: Application
    Filed: March 17, 2022
    Publication date: May 9, 2024
    Applicant: Merck Sharp & Dohme LLC
    Inventors: Pooja Chadha, Kent Harley Hamaker, Shannon Haughney, Samantha J. Marrone, Christopher Ton, Igor Zabrodin
  • Publication number: 20240150730
    Abstract: The present disclosure relates to a novel citrate synthase variant, a microorganism comprising the variant, and a method for producing O-acetyl-L-homoserine and L-methionine using the microorganism.
    Type: Application
    Filed: March 10, 2022
    Publication date: May 9, 2024
    Applicant: CJ CHEILJEDANG CORPORATION
    Inventors: Jin Sook CHANG, Seung Hyun CHO, Seo-Yun KIM, Jaemin LEE, Min Ji BAEK, Imsang LEE
  • Publication number: 20240150731
    Abstract: The present disclosure relates to a novel citrate synthase variant, a microorganism comprising the variant, and a method for producing L-amino acids using the microorganism.
    Type: Application
    Filed: March 10, 2022
    Publication date: May 9, 2024
    Applicant: CJ CHEILJEDANG CORPORATION
    Inventors: Jin Sook CHANG, Ju-yeon KIM, Seon Hye KIM, Hyung Joon KIM, Byoung Hoon YOON, Heeseok LEE