Abstract: A laminate production method is provided which can sufficiently prevent the occurrence of blocking without causing deterioration in the outstanding characteristics of acrylic block copolymers such as adhesive performance, and can also ensure excellent processability during extrusion.

Abstract: An epoxy-based adhesive composition and methods for manufacturing the same are provided. The adhesive composition provides excellent adhesion strength, peel strength and impact-resistant strength uniformly over a wide temperature range.

Abstract: The present invention refers to an adhesive composition for flexible lamination, especially for food packaging, which comprises a vegetable oil-based polyol and an NCO-terminated compound as well as a method of producing the vegetable oil-based polyol. Further, the present invention refers to the use of the inventive adhesive composition in flexible lamination and an article comprising the inventive adhesive composition.

Abstract: A device comprising an adhesive material and a fiberglass mesh located in direct planar contact with the adhesive material. The adhesive material is tacky in its green state and includes an epoxy, an epoxidized cashew nut shell oil, and at least about 20% by weight of a component containing a core shell polymer material to improve the adhesion capability to surfaces that are contaminated and/or exposed to high pressure spraying or chemical treatments, heat and humidity.

Abstract: Systems and films for protecting surfaces of various substrates, including electronic devices, such as portable electronic devices, include a liner with a strip liner and a main liner. The strip liner, which covers a small strip of adhesive on the back side of a protective film, is configured to be removed before the main liner. With the small strip of adhesive exposed, the protective film may be aligned with a substrate and, if necessary, removed from the substrates and repositioned thereon. Thereafter, the main liner may be removed to enable complete application of the protective film to the substrate. Methods for securing protective films to substrates are also disclosed.

Abstract: The invention relates to a hot-melt adhesive composition, comprising at least one polyolefin polymer at a content from 5 to 90% by weight; at least one branched polypropylene at a content from 0.1 to 10% by weight; at least one tackifying resin at a content from 0.1 to 60% by weight; optionally, at least one styrene block copolymer from 0 to 30% by weight; optionally, at least one plasticizer at a content from 0 to 30% by weight; optionally, at least one stabilizer at a content from 0 to 5% by weight; and optionally, at least one wax at a content from 0 to 30% by weight. The invention further relates to the use of a branched polypropylene for reducing stringing of a hot-melt adhesive composition.

Abstract: Provided is a hot-melt pressure-sensitive adhesive composition which enables to form a pressure-sensitive adhesive layer without allowing the progression of rapid gelation at the time of heating and melting. The pressure-sensitive adhesive composition provided by the present invention is a solvent-free hot-melt pressure-sensitive adhesive composition. The pressure-sensitive adhesive composition includes a base polymer, an isocyanate crosslinking agent, and a tackifier T which is a reaction product of a compound X and a compound Y. A content of the tackifier T with respect to 100 parts by weight of the base polymer is 20 parts by weight or more and 40 parts by weight or less.

Abstract: A polyester pressure-sensitive adhesive composition is provided including a polyester resin having a predetermined amount of a structural unit derived from a hydrogenated polybutadiene structure-containing compound, or a polyester resin having a structural unit derived from a hydrogenated polybutadiene structure-containing compound and a predetermined amount of a structural unit derived from an aromatic ring structure-containing compound. The polyester pressure-sensitive adhesive composition has superior adhesion to a polyolefin base material and is highly transparency.

Abstract: The present disclosure provides a laminate. The laminate includes a first film and a second film, wherein the first film is laminated to the second film. The first film includes a sealant layer containing (A) an ethylene-based polymer; and (B) a slip agent blend containing (i) a first polydimethylsiloxane having a number average molecular weight (Mn) from 30,000 g/mol to less than 300,000 g/mol; and (ii) a second polydimethylsiloxane having a number average molecular weight (Mn) from 300,000 g/mol to 2,000,000 g/mol.

Abstract: Curable adhesive films, primers for adhesion of curable adhesive films, and adhesive systems comprising adhesive films and primers are provided. In some embodiments, an adhesive system comprises: I) a curable adhesive free-standing film comprising a blend of: a) a first film-forming polymer or oligomer; b) a first species comprising first unsaturated free-radically polymerizable groups, which may be a) or a species other than a); and c) a first transition metal cation; and II) a primer for adhesion of a curable adhesive free-standing film to a first substrate comprising an oxidizing agent. In some embodiments, an adhesive system comprises: I) a curable adhesive free-standing film comprising an outer surface bearing embossed air bleed channels capable of aiding in escape of air during application of the outer surface to a primed substrate; and II) a primer comprising components that initiate cure of the curable adhesive free-standing film.

Abstract: The present invention relates to the field of luminescent crystals (LCs) of the core-shell type, components and devices comprising the same. Particularly, the present invention relates to the manufacturing of luminescent core-shell nanocrystals, wherein said nanocrystal core comprises or consists of a perovskite crystal having a size of 3-100 nm, and said nanocrystal shell comprises a metal oxide having a thickness of 0.5-10 nm and wherein said method comprises combining suitable starting materials under oxygen-free conditions. The invention further provides for specific luminescent core-shell nanocrystals, to components and products containing such luminescent core-shell nanocrystals.

Abstract: Interpolymers useful for enhancing the efficacy of nutrients in soil and fertilizers; and methods of inhibiting the precipitation of, and/or dispersing, inorganic elements.

Abstract: A mulch composition includes a blend of about 50-85 wt. % fibrous straw, based on the total weight of the composition; about 10-30 wt. % refined pulp fibers, based on the total weight of the composition; and about 10-40 wt. % fibrous wood components, based on the total weight of the composition, the blend having about 10-15 wt. % of particles having particle size of 2360 ?m or greater.

Abstract: The present invention relates to a dispersant derived from anhydride functionalized polyester derived from carboxylic acid functionalized polyester. The anhydride functionalized polyester is then reacted with a multi-amine species forming amide and salt bonds. The technology allows lower reaction temperatures when the multi-amine species is present. The lower reaction temperature allows the use of a broader selection of polyester repeat units.

Abstract: The present invention is directed to a liquid chemical for forming a water-repellent protective film on a silicon element-containing wafer surface, a method of preparing the liquid chemical and a method of manufacturing a surface-treated body with the use of the liquid chemical, wherein the liquid chemical includes the following components: (I) a silylation agent; (II) at least one kind of nitrogen-containing compound selected from the group consisting of those of the following general formulas [1] and [2]; and (III) an organic solvent.

Abstract: A heat storage composite material comprises components by weight: 30-55 parts of organic phase change material, 30-40 parts of two-dimensional thermally conductive carbon material, 10-20 parts of lamellar structure graphite, and 0-10 parts of oil-absorbing organic resin. A preparing method include steps of stirring the organic phase change material to disperse on a surface of the two-dimensional thermally conductive carbon material, and melting them so the organic phase-change material is adsorbed in gaps of the two-dimensional thermally conductive carbon material; stirring and mixing the lamellar structure graphite and the two-dimensional thermally conductive carbon material adsorbed with the organic phase change material in a mixer to obtain a mixed material; and placing the mixed material in a lamination mold for lamination treatment to obtain a sheet-shaped heat storage composite material. The heat storage composite material has high thermal conductivity and is not easy to leak.

Abstract: A variety of methods and compositions are disclosed, including, in one example, a method that includes introducing a drill-in fluid into a production zone of a subterranean formation, where the drill-in fluid includes water, a hydrophobically modified starch, a dual oil and water soluble surfactant, and a bridging agent, extending a borehole through the production zone, and forming a filter cake in the production zone where the filter cake includes a plurality of oleophilic channels is formed within the filter cake.

Abstract: A solids-control fluid for controlling flow of solids in a subterranean formation is disclosed herein. The solids-control fluid can include a diluent and a curable resin. The diluent can include a glycol ether. The curable resin can be dispersed within the diluent for controlling flow of solids in the subterranean formation.

Abstract: The present invention relates to artificially reducing the porosity of any potential flow paths in the near-wellbore region of a well or in permeable zones within or surrounding a well. In doing so, the permeability in this the targeted region will be significantly reduced, thus, preventing unwanted flow of subsurface fluids. The present invention concerns a method comprising applying a first and second solution comprising scale precursors to the porous media, wherein following this application, at least a portion of the scale precursors form at least two insoluble salts. Additionally, the present invention concerns a kit of parts comprising the first and second solutions.

Abstract: The present disclosure provides for a sandstone-containing oil reservoir additive composition that includes (a) a nonionic wettability alteration agent having Formula (I): and (b) an injection fluid. For Formula (I), R is an alkyl residue from a branched secondary alcohol initiator, where R can have a carbon number of C4 to C10, x is from 3 to 10 and y is from 1 to 20. The injection fluid can be selected from the group consisting of sea water, engineered water, injection brine, produced brine or combinations thereof. The nonionic wettability agent in the sandstone reservoir additive composition can be from 0.01 weight percent (wt %) to 20 wt % based on the total weight of the sandstone reservoir additive composition. The sandstone-containing oil reservoir additive composition can be used in enhance oil recovery operations, such as a waterflooding operation or a carbon dioxide flooding operation.

Abstract: The present invention relates to a method for modifying the water permeability of a subterranean formation which comprises oil, said method comprising at least the following steps: Preparing an injection fluid from a dispersion of a hydrophilic phase in a lipophilic phase, with water or brine, the dispersion comprising: a hydrophilic phase comprising at least one linear (co)polymer E, a lipophilic phase, at least one interface polymer composed of at least one monomer of formula (I): Injecting the injection fluid into the subterranean formation, comprising a linear (co)polymer E concentration, such that when released and in contact with water, the viscosity of the injection fluid is greater than the viscosity of the oil in the formation.

Abstract: A polyacrylamide friction reducer wherein the friction reducer contains surfactant functional groups, and wherein the polyacrylamide friction reducer is degradable, can be used for multi-functional, slickwater fracturing fluid projects. Under downhole conditions, the polymer degrades and releases the surfactant molecules to act as oil displacing agent to provide additional stimulation.

Abstract: A polyacrylamide friction reducer wherein the friction reducer contains surfactant functional groups, and wherein the polyacrylamide friction reducer is degradable, can be used for multi-functional, slickwater fracturing fluid projects. Under downhole conditions, the polymer degrades and releases the surfactant molecules to act as oil displacing agent to provide additional stimulation.

Abstract: The present disclosure relates to a processes and systems for producing fuels from biomass with high carbon conversion efficiency. The processes and systems described herein provide a highly efficient process for producing hydrocarbons from biomass with very low Green House Gas (GHG) emissions using a specific combination of components, process flows, and recycle streams. The processes and systems described herein provide a carbon conversion efficiency greater than 95% with little to no GHG in the flue gas due to the novel arrangement of components and utilizes renewable energy to provide energy to some components. The system reuses water and carbon dioxide produced in the process flows and recycles naphtha and tail gas streams to other units in the system for additional conversion to syngas to produce hydrocarbon-based fuels.

Abstract: The present invention is generally directed to processes and systems for the purification and conversion of CO2 into low-carbon or zero-carbon high quality fuels and chemicals using renewable energy. In one aspect, the present invention provides a process for producing a stream comprising at least 90 mol % CO2. In certain cases, the CO2 stream is processed to make low carbon fuels and chemicals. In this process at least a portion of the CO2 is reacted with a stream comprising H2 in a Reverse Water Gas Shift (RWGS) reactor to produce a product stream that comprises CO.

Abstract: Systems and methods to enhance the removal of inorganic contaminants, including metals, from hydrocarbon feedstocks at a refinery. One or more embodiments of such systems and methods may be used to provide a renewable hydrocarbon feedstock having a reduced amount of metal contaminants. The reduction of metal contaminants in the renewable hydrocarbon feedstock mitigates catalyst fouling and/or deactivation during downstream refinery processing of the renewable hydrocarbon feedstock.

Abstract: A process for capturing carbon dioxide includes the steps of mixing a hydrogen stream and a feedstock stream to produce a mixed stream, wherein the feedstock stream includes hydrocarbons, reacting the hydrocarbons and the hydrogen in the primary reactor of the hydroprocessing unit to produce a hydroprocessing product stream and a carbon dioxide stream, wherein the hydroprocessing product stream includes light products, wherein the hydroprocessing unit is further configured to produce ammonium bisulfide, collecting the ammonium bisulfide in the water to produce a sour water, processing the sour water in the waste water unit to produce an ammonia stream, a hydrogen sulfide stream, and a stripped water stream, introducing the ammonia stream to a carbon dioxide recovery system, and separating carbon dioxide from the carbon dioxide stream using the ammonia in the ammonia stream to produce a carbon dioxide product.

Abstract: A method for combined production of renewable paraffinic products is disclosed, wherein the method includes providing a renewable paraffinic feed, and fractionating the renewable paraffinic feed into two fractions. Within the two fractions, a lighter fraction fulfils a specification for an aviation fuel component, and a heavier fraction fulfils a specification for an electrotechnical fluid component.

Abstract: The present invention relates to a process for producing olefins from a hydrocarbon feedstock 11 having a sulfur content of at least 0.1 weight %, an initial boiling point of at least 180° C. and a final boiling point of at least 600° C.

Abstract: A non-petroleum or renewable feedstock containing oxygen and contaminants of metals, gums, and resins is treated by introducing the feedstock into a reactor at a flow velocity of at least 20 ft/sec. The feedstock is heated within the reactor and cooled to form a reduced-temperature reactor product. At least a portion of the reduced-temperature reactor product is feed into a hydroprocessing reactor containing a hydroprocessing catalyst to form a hydroprocessed product. The hydroprocessed product is cooled and non-condensable gases, metals and water are separated and removed to form a final product. The final product has an oxygen content that is 60% or less of that of the feedstock, and wherein the final product comprises 25 wt % or less any triglycerides, monoglycerides, diglycerides, free fatty acids, phosphatides, sterols, tocopherols, tocotrienols, or fatty alcohols, from 5 wt % to 30 wt % naphtha, and 50 wt % or more diesel.

Abstract: Processes herein may be used to thermally crack various hydrocarbon feeds, and may eliminate the refinery altogether while making the crude to chemicals process very flexible in terms of crude. In embodiments herein, crude is progressively separated into at least light and heavy fractions. Depending on the quality of the light and heavy fractions, these are routed to one of three upgrading operations, including a fixed bed hydroconversion unit, a fluidized catalytic conversion unit, or a residue hydrocracking unit that may utilize an ebullated bed reactor. Products from the upgrading operations may be used as feed to a steam cracker.

Abstract: This invention relates to drag reducing agents used in case of transportation of crude oil products, in particular, to solid dry poly(meth)acrylate with dry poly alpha-olefins powders based agents. The technical result of the solution involves the preparation of an effective, fast-dissolving, stable solid dry drag reducing agent suitable for use in heavy crude oil. The drag reducing agents compositions have better drag reducing performance in heavy crude applications as compared with traditional drag reducing slurries is also disclosed, which consists of the following components, wt %: a mixture of at least two drag reducing polymers, from 65 to 90 a separating agent from 3 to 25 anti-agglomerating agent from 3 to 25 a surfactant from 3 to 25, where the first polymer is poly(meth)acrylate, the second polymer is poly alpha-olefin, the ratio of the polyacrylate weight and polyalphaolefin weight varies in the range from 2:1 to 5:1.

Abstract: Conduits for cooling a hydrocarbon stream and processes for using same. The conduit can include a first inner wall defining a first bore, a second inner wall defining a second bore, and an outer wall disposed about the first and second inner walls. The conduit can also include an annular support wall connected to an inner surface of the outer wall. An end of the second inner wall and an end of the annular support wall can define a perimeter opening that can be in fluid communication with the second bore. An annular flexible ring can be bonded to the annular support wall and can flexibly contact the first inner wall. A substantially annular cavity can be disposed between the second inner and the outer walls and in fluid communication with the perimeter opening. A quench fluid introduction port can be configured to introduce a quench fluid into the cavity.

Abstract: A hydrocarbon cracker stream is combined with recycle content pyrolysis oil to form a combined cracker stream and the combined cracker stream is cracked in a cracker furnace to provide an olefin-containing effluent. The r-pyoil can be fed to the cracker feed. Alternatively, the r-pyoil with a predominantly c8+ fraction can be fed to the cracker feed. The furnace can be a gas fed furnace, or split cracker furnace.

Abstract: A diesel fuel composition as disclosed includes a fossil diesel component, a fatty acid methyl esters component, and a hydrotreated renewable paraffinic diesel component. A method is disclosed for producing the diesel composition and use thereof. Use of the hydrotreated renewable paraffinic diesel component as an oxidation stability improver is further disclosed.

Abstract: A method for forming a magnetic hydrochar nanocomposite includes the step of adding basically treated desert date seeds into a vessel containing phosphoric acid and mixing the basically treated desert date seeds with the phosphoric acid to form a mixture. The mixture is transferred to a microwave and heated to 150° C. The mixture is held at this temperature for one hour to form a hydrochar. The magnetic hydrochar nanocomposite is further formed by dissolving ferrous sulphate and ferric chloride to form magnetic nanoparticles. The magnetic nanoparticles are mixed with the hydrochar, then date fruit extract is mixed with the magnetic nanoparticles and hydrochar mixture to form a resultant mixture. The pH of the resultant mixture is raised to 9-11 by adding sodium hydroxide to form a basic resultant mixture. The basic resultant mixture is heated in a microwave to 150° C. for 30 minutes to form the magnetic hydrochar nanocomposite.

Abstract: A lubricant base oil of the present invention includes an ester compound including: a structural moiety (A) that is derived from an alcohol compound (A); and a structural moiety (B) that is derived from a carboxylic acid, the structural moiety B including: a structural moiety (B1) that is derived from a monocarboxylic acid (B1); and a structural moiety (B2) that is derived from an aliphatic monocarboxylic acid (B2) having 4 or more and 18 or less carbon atoms. According to the present invention, it is possible to provide a lubricant base oil having excellent low-temperature stability and high heat resistance while maintaining torque-lowering properties.

Abstract: A lubricating oil composition comprises 50 mass % or more of an oil of lubricating viscosity and 0.1 to 25 mass % of at least one of compounds of structures (I), (II), or (III). wherein X1 and X2 are the same or different and are OH, NH2 or SH; wherein groups R1 and R2 are the same or different and are linear or branched, saturated or unsaturated hydrocarbon groups having from 1 to 50 carbon atoms, with the proviso that at least one of groups R1 and R2 has at least 4 carbon atoms; and wherein m and n are the same or different and are zero or an integer from 1 to 3 with the proviso that m and n are not both zero. The compositions are useful to ameliorate or prevent deposits in engines and provide good asphaltene dispersancy.

Abstract: An additive for lubricating oil may include a phosphite ester compound (A) having a specific structure and a phosphite ester compound (B) having a specific structure, wherein the (A):(B) mass ratio of the phosphite ester compound (A) to the phosphite ester compound (B) is in a range of from 99:1 to 80:20. Such an additive may be provided to a lubricating oil, capable of imparting wear resistance and demulsibility with respect to a base oil for lubricating oil, and to impart durability even in the presence of water.

Abstract: A polyester is useful as a high viscosity base fluid. A method for its preparation involves epoxidizing an alpha-olefin, reacting the alpha-epoxide or diol with a saturated linear aliphatic dicarboxylic acid, and isolating the polyester. Lubricant compositions can contain such polyesters and the compositions are useful as automatic transmission fluids, manual transmission fluids, continuously variable transmission fluids, gear oil formulations, industrial gear oil formulations, axle fluid formulations, dual clutch transmission fluids, dedicated hybrid transmission fluids, or as hydraulic oils.

Abstract: The instant disclosure relates to a lubricant composition having an oil of lubricating viscosity, a polymeric surfactant compound that is an acylated polymer having a number average molecular weight of about 500 to about 50,000 where the polymer comprises a branched olefin having from 8 to 30 carbon atoms. The acylated polymer can further be reacted with an amine or alcohol to form an amide, imide, ester or combinations thereof. The present disclosure further relates to lubricant compositions that exhibit good dispersancy and viscometric performance.

Abstract: An aspect of the present invention provides a refrigerating machine oil containing a mixed base oil of a first hydrocarbon base oil having a kinematic viscosity at 40° C. of less than 6 mm2/s and a second hydrocarbon base oil having a kinematic viscosity at 40° C. of 6 mm2/s or more, wherein a (A)/(B) ratio is more than 1 and 1.5 or less, wherein (A) is a kinematic viscosity at 40° C. of the mixed base oil, and (B) is the kinematic viscosity at 40° C. of the first hydrocarbon base oil.

Abstract: The present disclosure relates to lubricating compositions configured for motorcycles and also to methods of lubricating a motorcycle engine, transmission, and clutch assembly with a single lubricating composition that is generally provided from a common sump or reservoir to achieve improved fuel economy.

Abstract: A lubricating composition includes at least one base oil, at least one borated dispersant, at least two antioxidants selected from amine antioxidants, phenolic antioxidants substituted with one or more linear or branched alkyl groups having from 1 to 30 carbon atoms, and mixtures thereof, and at least one phosphite polymer having the formula (I) wherein: —each of the R1, R2, R3 and R4 can be independently selected from the C1-C20 alkyl, C3-C22 alkenyl, C6-C40 cycloalkyl, C7-C40 cycloalkenyl, C1-20 methoxy alkyl glycol ethers and Y—OH groups; —Y is selected from C2-C40 alkylene, C2-C40 alkyl lactone, —R7—N(R8)—R9— groups, in which R7, R8 and R9 are independently selected from hydrogen, C1-C20 alkyl, C3-C22 alkenyi, C6-C40 cycloalkyl, C7-C40 cycloalkenyl, C1-20 methoxy alkyl glycol ethers; —m is an integer from 2 to 100; —n is an integer from 1 to 1000.

Abstract: A method of removing metal particles from a contaminated metalworking fluid comprising emulsion droplets and metal particles includes pressurizing a first clean metalworking fluid with gas to provide an aerated metalworking fluid; releasing the pressure of the aerated metalworking fluid to form a plurality of bubbles; applying a shear force to the contaminated metalworking fluid to separate the emulsion droplets from the metal particles; flowing the contaminated metalworking fluid with the aerated metalworking fluid in a laminar flow to form a combined fluid, wherein the flowing occurs during the formation of the plurality of bubbles and while the emulsion droplets are separated from the metal particles, and wherein the laminar flow lasts for a time sufficient for the plurality of bubbles to attach to the metal particles; releasing the combined fluid into a flotation tank; and removing the metal particles to form a second clean metalworking fluid.

Abstract: An edible composition, an aerosol composition, a flavor composition, a fragrance composition, or an inhalable composition includes an organosulfur compound such as prenyl mercaptan, 2-methylthiophene, 3-methylthiophene, dimethyl disulfide, diprenyl disulfide, 3-methyl-2-buten-1-yl thiolacetate, 3-methyl-1-[(3-methyl-2-buten-1-yl)sulfanyl]-2-butene, prenylmethylthiol (1-(methylsulfanyl)-3-methyl-2-butene), prenyl thioacetate, thiogeraniol, dimethyl sulfide, or a combination of any two or more thereof, and a primary terpene compound selected from the group consisting of myrcene, ?-caryophyllene, limonene, ?-pinene, ?-pinene, valencene, ocimene, terpinolene, or a combination of any two or more thereof.

Abstract: The invention relates to a device and process for converting organic waste to biogas with high methane content and improved organic conversion efficiency. Disclosed process consists of two stages and in the first stage, shredded organic waste is digested in primary digester in which biodegradable organic fractions present in waste gets converted to volatile fatty acids and alcohols dissolved in aqueous solution by hydrolytic and acidogenic microorganisms. Primary digester effluent pH, is adjusted to about 6.8-7.5 by addition of controlled alkali solution. Neutralized waste slurry is separated into liquid solution called as leachate and digested solid sludge. In the second stage, liquid leachate comprising volatile fatty acids are converted to biogas with methane content in the range 80-86% by methanogenic microbial culture in main digester under anaerobic conditions.

Abstract: An in vitro microfluidic “organ-on-chip” device is described herein that mimics the structure and at least one function of specific areas of the epithelial system in vivo. In particular, a stem cell-based Lung-on-Chip is described. This in vitro microfluidic system can be used for modeling differentiation of cells on-chip into lung cells, e.g., a lung (Lung-On-Chip), bronchial (Airway-On-Chip; small-Airway-On-Chip), alveolar sac (Alveolar-On-Chip), etc., for use in modeling disease states of derived tissue, i.e. as healthy, pre-disease and diseased tissues. Additionally, stem cells under differentiation protocols for deriving (producing) differentiated lung cells off-chips may be seeded onto microfluidic devices at any desired point during the in vitro differentiation pathway for further differentiation on-chip or placed on-chip before, during or after terminal differentiation.

Abstract: Zebrafish are a powerful model for investigating cardiac repair due to their unique regenerative abilities, scalability, and compatibility with many genetic tools. However, characterizing the regeneration process in live adult zebrafish hearts has proved challenging because adult fish are opaque and explanted hearts in conventional culture conditions experience rapid declines in morphology and physiology. To overcome these limitations, we fabricated a fluidic device for culturing explanted adult zebrafish hearts with constant media perfusion that is also compatible with live imaging. Unlike hearts cultured in dishes for one week, the morphology and calcium activity of hearts cultured in the device for one week were largely similar to freshly explanted hearts.

Abstract: The present disclosure describes hydrogels which are micropatterned with a network of wells for cell culture. In a preferred embodiment, the micropatterned hydrogels are embedded with a nanomaterial. Further described are methods of forming the micropatterned hydrogels and methods of culturing cells in the micropatterned hydrogels. The hydrogels can be natural or synthetic.