Abstract: Provided are gene polymorphism markers having correlation significance with skin turnover and hyper-keratinization, a composition for determining skin turnover and hyper-keratinization, the composition including a probe capable of detecting the same or an agent capable of amplifying the same, a kit or microarray for determining skin turnover and hyper-keratinization, the kit or microarray including the composition, a method of providing information for determining skin turnover and hyper-keratinization using the gene polymorphism markers or a combination of the markers, and a method of controlling skin turnover and hyper-keratinization, the method including the steps of identifying the polymorphic site of the single nucleotide polymorphism marker and prescribing skin care products. Since the single nucleotide polymorphism markers of the present invention may provide accurate information about each individual's skin type, it is possible to develop personalized cosmetics and customized active ingredients.

Abstract: Methods for performing multiplex PCR-based enrichment of a target substrate are provided. Systems and methods for generating a sequencing library are also provided.

Abstract: The present disclosure provides compositions, methods, and systems for preparing nucleic acid samples for sequencing, including attaching adapters to template inserts, attaching template nucleic acid molecules to a support, enrichment. Further provided herein are methods of error correction by preserving both strands of a template nucleic acid molecule during amplification.

Abstract: The present disclosure provides method and systems for improving nanopore-based analyses of polymers. The disclosure provides methods for selectively modifying one or more monomeric subunit(s) of a kind a pre-analyte polymer that results polymer analyte with a modified subunit. The polymer analyte produces a detectable signal in a nanopore-based system. The detectable signal, and/or its deviation from a reference signal, indicates the location of the modified subunit in the polymer analyte and, thus, permits the identification of the subunit at that location in the original pre-analyte polymer.

Abstract: The invention relates to methods for use in nucleic acid sequencing, in particular methods for use in concurrent sequencing.

Abstract: Methods and systems for detecting cancer and/or determining a cancer tissue of origin are disclosed. In some embodiments, a multiclass cancer classifier is disclosed that is trained with a plurality of biological samples containing cfDNA fragments. The analytics system derives a feature vector for each sample, and the multiclass classifier predicts a probability likelihood for each of a plurality of cancer signal origin (CSO) classes. In some embodiments, the plurality of CSO classes include hematological subtypes, including both hematological malignancies and precursor conditions. In one embodiment, non-cancer samples having high prediction score are pruned from the training sample set. In another embodiment, the analytics system stratifies samples according to prediction score and applies binary threshold cutoffs determined for each stratum.

Abstract: Provided are systems and methods for analyte detection and analysis. A system can comprise an open substrate. The open substrate may be configured to rotate or otherwise move. The open substrate can comprise an array of individually addressable locations, with analytes immobilized thereto. The substrate may be spatially indexed to identify nucleic acid molecules from one or more sources, and/or sequences thereof, with the respective one or more sources. A solution comprising a plurality of probes may be directed across the array to couple at least one of the plurality of probes with at least one of the analytes to form a bound probe. A detector can be configured to detect a signal from the bound probe via scanning of the substrate while minimizing temperature fluctuations of the substrate or optical aberrations caused by bubbles.

Abstract: This disclosure provides oligomers, combinations of oligomers, compositions, kits, uses, and methods for detecting a C1orf43 nucleic acid, such as C1orf43 mRNA, such as human C1orf43 mRNA, in a sample.

Abstract: Method for predicting a severity of an infectious disease and biomarker for use in carrying out the method and monitoring a therapy of an infectious disease using an RT-qPCR method, while the method is being performed on a nasopharyngeal swab sample determining the amount of serum amyloid A mRNA, preferably SAA1, and the amount of serum amyloid A mRNA is normalized to the amount of mRNA of a constitutively expressed gene, which is preferably UBC. Based on the determined normalized value of the amount of SAA1 mRNA in the sample, the severity of the course of an infectious disease, which may be of viral, bacterial, or fungal origin, is predicted, and the effectiveness of a therapy of the given disease is further monitored.

Abstract: Method of determining if a subject has suffered tissue damage from exposure to a toxic agent. The method comprises sequencing cell-free DNA (cfDNA) in a biospecimen from the subject; determining cellular origin of the cfDNA by identifying methylation patterns in one or more portions of the sequence of the cfDNA that contains methylation sites, in which the cellular origin of the cfDNA is determined when the methylation pattern in the one or more portions is the same as a known cell-type specific methylation patterns; measuring the quantity of the cfDNA of the determined cellular origin, and comparing the measured quantity of the cfDNA of the determined cellular origin with a normal quantity of cfDNA of the determined cellular origin. A greater quantity of the measured cfDNA of the determined cellular origin is indicative that the subject has suffered tissue damage.

Abstract: This disclosure relates more particularly to risk assessment of preterm delivery (PTD) in the subject and designing a personalized recommendation for the same. Conventional techniques for PTD risk assessment are either invasive or minimally invasive and leaves little time for subjects to take precautionary or corrective medical advice or procedures to reduce or obviate the risk. The present disclosure provides the risk assessment of the PTD, by quantifying a microbial abundance in oral or gut microbiome for a pregnant woman, identifying a certain combination of microbial biomarkers using an ensemble of models for accurate risk assessment of the PTD and subsequently suggesting a personalized recommendation for at risk subject. The present assessment technique is completely non-invasive and further helps in characterizing the risk of the PTD.

Abstract: The present results show that KDM1A is a key epigenetic regulator of tissue-specific expression of GIP receptor and possibly of other receptors from the G-protein coupled receptor family in hormone-producing glands, and that its alteration leads to the development of aberrant overexpression of eutopic hormone receptors or expression of ectopic hormone receptors that lead to abnormal steroidogenesis. They also show that loss of expression of KDM1A is likely to be the initiating event that trigger the abnormal cell proliferation leading to the development of tissue lesions in adrenal and possibly in other endocrine tissues (notably in the adrenal glands). The present invention therefore proposes to detect altered expression of KDM1A in the genome of subjects, in order to diagnose a genetic predisposition to an endocrine disease and/or to an endocrine hyperplasia.

Abstract: The present disclosure relates to a method for identifying and diagnosing genetic disorders. The method comprises obtaining a sample from a subject. The method also comprises extracting deoxyribonucleic acid (DNA) from the sample collected. The method also comprises storing properly the extracted deoxyribonucleic acid (DNA) from the collected sample. The method also comprises analyzing for the presence of a genetic mutation or a genetic profile associated with the disorder. The method also comprises comparing the identified mutation or profile with a database of known mutations or profiles associated with various genetic disorders. The method also comprises providing probable diagnosis of the disorder based on the comparative results obtained.

Abstract: Techniques for receiving a voice command from a user and, in response, providing audible content to the user via a first device and providing visual content for the user via a second device. In some instances, the first device includes a microphone for generating audio signals that include user speech, as well as a speaker for outputting audible content in response to identified voice commands from the speech. However, the first device might not include a display for displaying graphical content. As such, the first device may be configured to identify devices that include displays and that are proximate to the first device. The first device may then instruct one or more of these other devices to output visual content associated with a user's voice command.

Abstract: Methods and compositions are provided for detecting a predisposition for cardiovascular disease in an individual.

Abstract: The first genome-wide, large-cohort study to demonstrate donor kidney transcriptomes can capture intrinsic organ quality and carry significant predictive weight for 24-month transplant function is disclosed. These findings shift the paradigm of understanding longer-term kidney transplant outcomes away from recipient factors/post-transplant events and towards intrinsic donor organ quality, which can be captured by molecular techniques. The combined predictive equation provided herein, using both clinical and biological data, can more accurately predict 24-month outcomes as compared to the current established scoring system (KDPI) in an external patient cohort.

Abstract: Disclosed herein are methods for evaluating the efficacy of an immunogenic composition by monitoring circulating tumor DNA. The disclosure also relates to methods for treating a subject having cancer.

Abstract: The present disclosure relates generally to methods for the analysis of gene expression. In particular, the methods of the present disclosure are based on the measurement of gene expression from fragments of cell-free DNA (cfDNA), which is useful in non-invasive methods for monitoring disease status in cancer patients and in methods for the treatment of cancer patients.

Abstract: The present invention relates to methods, systems and kits for the diagnosis, prognosis and the determination of progression of cancer in a subject. The invention also provides biomarkers that define subgroups of prostate cancer, clinically useful classifiers for distinguishing prostate cancer subtypes, bioinformatic methods for determining clinically useful classifiers, and methods of use of each of the foregoing. The methods, systems and kits can provide expression-based analysis of biomarkers for purposes of subtyping prostate cancer in a subject. Further disclosed herein, in certain instances, are probe sets for use in subtyping prostate cancer in a subject. Classifiers for subtyping a prostate cancer are provided. Methods of treating cancer based on molecular subtyping are also provided.

Abstract: Provided herein are methods based on MRI data with genetic alterations of tumors in the determination of severity and provision of treatment options for bladder cancer patients.

Abstract: Methods to quantify methylation in a DNA sample include treating the sample to encode the presence or absence of DNA methylation, adding to the sample a set of synthetic molecules (e.g., quality control template (QCT) molecules), generating a co-amplification mixture, sequencing the co-amplification mixture, and determining a number of methylated molecules in the sample based on the number of methylated reads from the sample and a number of reads from the set of synthetic molecules.

Abstract: A primer composition, a kit and a method for detecting polymorphic STR markers based on capillary electrophoresis and applications thereof are provided, which are used to simultaneously amplify 13 STR loci on cattle genome. The primer composition includes one or more pairs of primers with sequences as shown in SEQ ID NO: 1˜26. Developmental validation indicated that the kit is of high sensitivity, fine specificity, strong stability and high accuracy. This provides an effective tool for species identification, individual identification and parental analysis of cattle samples in civil disputes and illegal crimes.

Abstract: The present disclosure relates to systems and methods for for diagnosing medical conditions using microbiome diagnostics by collecting a sample from a patient, isolating microbial strains from the sample by placing the sample on a culture medium and incubating, analyzing the isolated microbial strains to detect the presence of certain pathogens using polymerase chain reaction (PCR) or other suitable analytic methods to detect the presence of pathogens including bacteria, viruses, fungi, or other microbes, interpreting the results to determine if the patient has a medical condition and providing a diagnosis based on the interpretation of the results.

Abstract: Disclosed herein are systems and methods for identifying biomarkers. Biomarker identification can be achieved while increasing efficiency and decreasing data and computation complexity but maintaining accuracy. Such biomarker identification can be achieved via analysis of differential gene expression, such as determined using single cell RNA-sequencing data sets.

Abstract: Disclosed in the present application are primers, probes and kits for multiplex detection of respiratory pathogen target nucleic acids, and methods of use thereof. The primers, probes and kits for multiplex detection of respiratory pathogen target nucleic acids and methods of use thereof, include two or more of the following: specific primers and probes for genes of influenza A virus, influenza B virus, human metapneumovirus, human coronavirus HKU1, parainfluenza virus type 1, parainfluenza virus type 2, parainfluenza virus type 3, parainfluenza virus type 4, respiratory syncytial virus type A, respiratory syncytial virus type B, adenovirus and enterovirus EV. The primers, probes, kits and methods provided by the present disclosure realize the simultaneous detection of a plurality of target nucleic acids, greatly improve the detection throughput, significantly reduce the detection time and cost, and improve both specificity and sensitivity of the detection.

Abstract: A method for dyeing and/or retanning of leather objects includes providing a leather object. The leather object has been processed by at least a tanning process. The leather object is introduced into a process chamber. The leather object is subjected in the process chamber to a pressurized fluid. The pressure of the pressurized fluid is controlled to reach a supercritical state. The leather object is subjected to a dyeing agent and/or a retanning agent, which dyeing or retanning agent is dissolved and/or transported in the pressurized fluid for at least a predefined period of time while the pressurized fluid is in the supercritical state. The pressure is reduced in the process chamber. The leather object is removed from the process chamber. The pressure reduction is controlled over time.

Abstract: A method of recovering enriched iron fines from bauxite waste includes calcining particles of bauxite waste to form oxygen carrier particles, subjecting the oxygen carrier particles to chemical looping combustion at a temperature of about 950° C.-1,050° C. for energy production and to produce the enriched iron fines as a by-product from the oxygen carrier particles via natural attrition and collecting the enriched iron fines.

Abstract: A method for producing iron containing products includes: operating a blast furnace plant to produce liquid pig iron from blast furnace charge material, whereby metallurgical gas having blast furnace top gas is generated; operating a direct reduction plant to produce direct reduced iron products from iron ore loaded into the top of a direct reduction furnace, a stream of reducing gas being introduced into the direct reduction furnace, the direct reduction plant including a reformer or heater device from which the stream of reducing gas is discharged, whereby top gas is generated by the direct reduction furnace; where a first stream of direct reduction plant top gas is treated in an enriching stage configured for enriching in reducing species, and forwarded to the blast furnace plant to be used therein as reducing gas; and where a first stream of the metallurgical gas (B3/B6) is forwarded to the reformer or heater device of the direct reduction plant to be used therein as fuel gas.

Abstract: The present invention provides an aluminum alloy deoxidizer with carbon compounds, composed of multiple aluminum pellets. The main component of the aluminum pellets is elemental aluminum, and the aluminum pellets includes a weight percentage of 0.1 to 8 of carbon or organic compounds.

Abstract: A metal-sheet quenching apparatus cools a metal sheet while conveying the metal sheet and includes a cooling tank in which a cooling fluid is to be stored and the metal sheet is immersed in the cooling fluid to be cooled; restraining rolls that are installed inside the cooling tank and convey the metal sheet that has been cooled in the cooling tank while restraining the metal sheet in the thickness direction; a water-level adjustor that adjusts the height of a fluid surface of the cooling fluid inside the cooling tank; and a position control device that adjusts the height of the fluid surface of the cooling fluid inside the cooling tank by controlling the operation of the water-level adjustor.

Abstract: A stainless steel sheet has a surface quality in which a core height Sk is 1.50 ?m or less, a reduced peak height Spk is 0.20 ?m or less, and an areal material ratio Smr2 that separates a reduced dale from a core is 80% or less, where the core height Sk, the reduced peak height Spk, and the areal material ratio Smr2 are as specified in JIS B 0681-2:2018. The stainless steel sheet has a whiteness of 50 or greater and an image clarity of 1% or greater.

Abstract: A metal-sheet quenching apparatus that cools a metal sheet while conveying the metal sheet. The metal-sheet quenching apparatus includes a cooling device that cools a metal sheet that is conveyed, restraining rolls that convey the metal sheet cooled by the cooling device while restraining the metal sheet in the thickness direction, a roll moving device that moves the restraining rolls in the conveyance direction of the metal sheet, and a movement control device that controls the operation of the roll moving device to adjust the position of the restraining rolls.

Abstract: A steel sheet contains, in mass %, C: 0.15 to 0.45%, Si: 1.5% or less, Mn: 1.7% or less, P: 0.03% or less, S: less than 0.0020%, sol. Al: 0.20% or less, N: 0.005% or less, B: 0.0015 to 0.0100%, and at least one of Nb and Ti in a total amount of 0.005 to 0.080%, with the balance being Fe and incidental impurities. The area fraction of martensite with respect to the total area of the microstructure is 95 to 100%, and the diameter of prior ? grains is less than 11.0 ?m. The number density A of precipitates having an equivalent circular diameter of 500 nm or more satisfies the formula: A (particles/mm2)?8.5×105×[B].

Abstract: The steel sheet comprising a chemical composition containing C, Si, Mn, P, S, Al, N, Ti, Nb, and B, with the balance being Fe and inevitable impurities, and satisfying predetermined formula, in which the total area ratio of martensite and bainite is 95% or more, the average grain size of prior austenite grains is 10 ?m or less, the B concentration at a prior austenite grain boundary is 0.10% or more in mass %, a C-concentrated region is provided along a martensitic grain boundary, and the C-concentrated region has a C concentration of 4.0 times or more than the C content in the steel, and the C-concentrated region has a concentration width of 3 nm or more and 100 nm or less in a direction perpendicular to the martensitic grain boundary and a length of 100 nm or more in a direction parallel to the martensitic grain boundary.

Abstract: The present invention relates to steel suitable for a pressure vessel that can be used as petrochemical manufacturing equipment, a storage tank, and the like, and, more specifically, to steel having excellent hydrogen-induced cracking (HIC) resistance and low-temperature impact toughness and a method for manufacturing same.

Abstract: A method for producing high strength three piece steel can body and cans produced therewith.

Abstract: A system and device for laser shock peening device and components thereof. The system includes umbilical cords with flexible outer sheaths surrounding the fiber optic cable within the umbilical. The umbilical may include two different sections with different outer sheathes. The applicator device may have a laser peeing pen that has a longitudinal axis that is spaced from the longitudinal axis of the applicator device. In a method of operating the device, a first step includes a vertical movement, followed by a second step of rotation movement, and then another step of vertical movement opposite the first step.

Abstract: The present invention relates to a method for recovering magnesium from sediment generated in an electrolytic chlorine generation system using seawater or brackish water, the method comprising the steps of: eluting magnesium by using sulfuric acid in magnesium hydroxide, which is sediment generated in an electrolytic chlorine generation system using seawater and brackish water; precipitating magnesium sulfate by adding an organic solvent to a magnesium-eluted solution; and after the precipitation of the magnesium sulfate, separating the organic solvent and sulfuric acid by using a vacuum evaporation method, and reusing the organic solvent.

Abstract: The present invention provides a high-strength composite modified aluminum alloy part and a preparation method therefor. The preparation method includes: providing an aluminum alloy melt; providing a modifier; adding the modifier to the aluminum alloy melt under an inert gas atmosphere and melting, to obtain a modified aluminum alloy melt; performing casting by using the modified aluminum alloy melt, to obtain the cast aluminum alloy blank; and performing a heat treatment on the modified aluminum alloy blank, wherein the heat treatment includes: a solution treatment, a water quenching treatment, and an aging treatment.

Abstract: Disclosed are a steel for a high-temperature carburized gear shaft and a manufacturing method for the steel. The steel for the high-temperature carburized gear shaft comprises the following chemical components in percentage by mass: 0.17-0.22% of C, 0.05-0.35% of Si, 0.80-1.40% of Mn, 0.010-0.035% of S, 0.80-1.40% of Cr, 0.020-0.046% of Al, 0.006-0.020% of N, 0.002-0.030% of Nb, V?0.02%, and Ti?0.01%. Also disclosed is a manufacturing method for the steel for the high-temperature carburized gear shaft, comprising the steps of: smelting and casting; heating; forging or rolling; and finishing. By reasonably controlling chemical element compositions of the steel, the steel for the gear shaft in the present invention can maintain proper austenite grain size and stability at high temperature, maintains 5-8 grades of the austenite grain size before and after the high-temperature vacuum carburizing at 940-1050° C.

Abstract: A preparation method for heterogeneous Mg alloys bar with high elastic modulus. It provides a preparation method of solid-liquid composite casting in a specific mold to produce the heterostructured metallic bars composed of high elastic modulus metal and low elastic modulus Mg alloy. Subsequently, the microstructure of heterogeneous Mg alloys bars is adjusted by the specific deformation and heat treatment. Heterogeneous Mg alloys bars without oxide inclusions and with good interfacial bonding were prepared through this method. The improvement of elastic modulus is obtained by tailoring the heterogeneous microstructure.

Abstract: The present invention provides a Zn—Mg—Al-based plated steel sheet having excellent in corrosion resistance and whiteness, and a method for manufacturing the same. And more specifically, the present invention provides a Zn—Mg—Al-based plated steel sheet having excellent in corrosion resistance, whiteness and bendability, and a method for manufacturing the same.

Abstract: The present invention provides a Zn—Mg—Al plated steel sheet having excellent in corrosion resistance and bendability, and a method for manufacturing same. And more specifically, the present invention provides a Zn—Mg—Al-based plated steel sheet having excellent in corrosion resistance, bendability, and plating adhesion, and a method for manufacturing the same.

Abstract: Embodiments of the disclosed subject matter provide an apparatus having a device with a micronozzle array disposed on a micro-fabricated fluidic die. The device may include a first gas distribution plate and a second opposing plate, where the micro-fabricated fluidic die is disposed between the first gas distribution plate and the second opposing plate, wherein the first gas distribution plate is irreversibly joined to the micronozzle array with a seal that is gas-tight, and where the first gas distribution plate includes a plurality of sealed flow paths. A manifold may be reversibly joined to the first gas distribution plate, where the micro-fabricated fluidic die and the first gas distribution plate and the second opposing plate are disposed between the manifold. A thermally conductive plate may have at least one window that provides a clearance fit for the device across a range of motion relative to the thermally conductive plate.

Abstract: This film-forming apparatus comprises a processing container, a sputtering target provided in the processing container, a pedestal having a mounting surface for mounting of a substrate in the processing container, a shutter member capable of covering the mounting surface, a conveyance mechanism for carrying in and carrying out the shutter member to/from the pedestal, a detection unit that is provided to the conveyance mechanism itself and detects an indicator relating to the presence/absence of the shutter member, and a processing unit for determining whether the shutter member is present/absent with respect to the conveyance mechanism on the basis of the detection result from the detection unit.

Abstract: A preparation device for an alloy target includes a material nozzle, a high-energy laser, and a target support substrate, the material nozzle and the high-energy laser are respectively arranged above the target support substrate. The preparation device uses the material nozzle to spray the material powder required for the alloy target to be prepared, the injection efficiency is independently adjusted to achieve the purpose of non-fixed composition of the alloy target, and the spatial position and angle of the material nozzle are independently adjusted to ensure uniform composition and density of the target, the high-energy laser beam generated by the high-energy laser is used to heat the material powder in the spraying area to form a target coating of the required composition, the target support substrate is used to support the target coating formed by the material powder.

Abstract: A chamber for a physical vapor deposition (PVD) apparatus includes a collimator configured to narrow filter sputtered particles into a beam, an electrostatic chuck configured to support a substrate in the chamber, a shield and a chamber plate. The chamber plate includes a nut plate portion having a plurality of nut plates and a plurality of cavities in the chamber plate that are configured to allow gas to ingress and egress, wherein the cavities and nut plates are provided in equal numbers. The chamber is configured to operate at a target pressure, and the number of nut plates and corresponding number of cavities are determined based on the target pressure.

Abstract: A temperature-controlled shield for an evaporation source is described. The temperature-controlled shield is configured to provide a pre-heating zone or a post-cooling zone.

Abstract: A method for embedding ruthenium in a recess formed on a surface of a substrate includes: forming a ruthenium layer in a region including a bottom portion of the recess by supplying a gas including, as a ruthenium precursor, a ruthenium compound that does not contain oxygen and carbon atoms to the substrate having a metal exposed on a bottom surface of the recess; and subsequently embedding ruthenium in the recess so as to cover the ruthenium layer by supplying a gas including, as a ruthenium precursor, Ru3(CO)12 to the substrate.

Abstract: In some implementations, one or more semiconductor processing tools may deposit cobalt material within a cavity of the semiconductor device. The one or more semiconductor processing tools may polish an upper surface of the cobalt material. The one or more semiconductor processing tools may perform a hydrogen soak on the semiconductor device. The one or more semiconductor processing tools may deposit tungsten material onto the upper surface of the cobalt material.