Abstract: The present invention is directed to a chloropropanol prevention pre-treatment process for crude vegetable oils.

Abstract: A laundry scent additive having polyethylene glycol repeat units and perfume. The laundry scent additive enables consumers to control the amount of scent imparted to their laundry.

Abstract: A treatment liquid contains water, hydroxylamine, and one or more kinds of hydrazines selected from the group consisting of hydrazine, a hydrazine salt, and a hydrazine derivative, in which a total content of the hydrazines is 1 part by mass or less with respect to 100 parts by mass of the hydroxylamine.

Abstract: The present inventive concept relates generally to simulating the barrel aging or finishing process of distilled spirits in a rapid manner.

Abstract: Disclosed herein is a process for the conditioning of wood barrels, in particular, a process for the rapid conditioning of wood barrels. The disclosed process may comprise one or more steps comprising: subjecting a wood barrel to heat to increase the temperature of the wood; contacting the heated internal surface of the wood barrel with a fluid additive; and then optionally: subjecting the interior of the wood barrel to a pressurised environment; and/or subjecting the wood barrel to further heating; repeating one or more steps; and allowing the wood barrel to cool, or cooling the wood barrel, to provide a conditioned wood barrel. Also disclosed is another process comprising the step of providing a bladder and a liquid additive within the interior of a wood barrel for a predetermined time to facilitate absorbing the additive into the wood, wherein the bladder reduces the interior volume of the wood barrel such that the liquid additive is in contact with the internal surface of the barrel.

Abstract: The cell encapsulation system (CES) device is a device used for dermal, subdermal, muscle, tissue, or organ implantation into an individual (host) that is capable of being loaded with and carrying and containing exogenously introduced cells (encapsulated cells) that can produce relevant biochemicals (factors) and/or therapeutic molecules that can be transported to the host tissue while simultaneously not eliciting a significant host immune response (to the implanted device or to the encapsulated cells). The CES device provides a means of local and/or systemic, prolonged delivery of single or multiple factors and/or therapeutic molecules to alleviate, treat, or cure a variety of acute and chronic pathologies and ailments.

Abstract: A device for treatment of cells with particles is disclosed. The device includes a semi-permeable membrane positioned between two plates, the first plate defining a first flow chamber and comprising a port, a flow channel, a transverse port, and a transverse flow channel, the first flow chamber constructed and arranged to deliver fluid in a transverse direction along the first side of the semi-permeable membrane, the second plate defining a second flow chamber and comprising a port. A method for transducing cells is disclosed. The method includes introducing a fluid with cells and viral particles into a flow chamber adjacent a semi-permeable membrane such that the cells and the viral particles are substantially evenly distributed on the semi-permeable membrane. The method also includes introducing a recovery fluid to suspend the cells and the viral particles, and separating the cells from the viral particles. A method of activating cells is disclosed.

Abstract: An Axial Dispersion Bioreactor (ADBR) is designed for the photoautotrophic, mixotrophic or heterotrophic growth and production of microalgae and other microorganisms (bacteria, fungi, etc.) as well as cell cultures of plants, animals, insects and others. The ADBR is equipped with a plate having a plurality of holes that moves longitudinally or axially within the bioreactor to effect superior hydrodynamic and mixing patterns. The ADBR has the advantages of providing a low-shear culture environment, superior liquid mixing, and efficient gas mass transfer.

Abstract: A magnetic-field generator (10) is provided to be used in construction of a cell sheet (12), and include s a magnetic circuit assembly (24) configured to generate a magnetic field for the construction of the cell sheet (12) using magnetic force caused by the magnetic field wherein the magnetic force is substantially transverse to a plane defined by the cell sheet (12), and a power control system (44) configured to generate and control electric power to magnetically charge the magnetic circuit assembly (24) using the electric power.

Abstract: The invention relates to a A bioprocess control device having a number of control function units (12, 30) which are accommodated in a device housing (10), are designed for bioprocesses and are configured for carrying out control, conveying, measuring and/or sensor functionalities and In order to accommodate the plurality of control function units, the device housing has modular drawer assemblies (14, 32) which, relative to stationary housing frame of the device housing in an opening position and/or removal position, allow access for configuration and/or assembly to the control function unit accommodated in a relevant drawer assembly, and, in an operating and closure position which can be locked by locking means (60, 58), produce an electrically supplying as well as electronically function-identifying contact between the control function unit and the housing frame and/or electrical power supply (40) as well as system identification means (42) associated with a further drawer assembly.

Abstract: Provided are methods and compositions for self-cleaning that include a digestive protein capable of decomposing stain forming molecules, a substrate applied to a solid surface, and a linker moiety bound to an outer surface of said substrate and an active group of said digestive protein, said linker moiety between said protein and said substrate and covalently linking said protein to a surface of said substrate by an amide bond, the linker moiety between a free amine of said protein and said outer surface of said substrate wherein the digestive protein forms a layer on a surface of said substrate such that the digestive protein is surface exposed for reaction with a stain.

Abstract: A well for electrically stimulating at least one cell. The well includes a bottom portion and comprises an adaptive electrode arrangement for introducing an electric field into the well. The adaptive electrode arrangement includes a pair of electrodes disposed within the well. Each electrode of the pair of electrodes has a distal end and is independently and axially displaceable relative to the other electrode and the bottom portion of the well. The distal end of each electrode of the pair of electrodes is in contact with the bottom portion of the well, ensuring a uniform and constant electric field is applied within the well.

Abstract: The present invention relates to a lysis, binding and/or wash reagent for isolating and/or purifying nucleic acids and a method for isolating and/or purifying nucleic acids.

Abstract: Described are compositions and methods relating to yeast cells having a genetic mutation that give rise to increased alcohol production, particularly under high dissolved solids growth conditions. Such yeast is well-suited for use in alcohol production to reduce fermentation time and/or increase yields.

Abstract: Disclosed herein include systems, methods, and compositions for determining perturbations in single cells or performing integrated measurements using, for example, plasmids each comprising (i) a perturbation gRNA with a guide region targeting a chromosome sequence and (ii) barcode gRNAs each with a guide region targeting predetermined spatial region of the genome and zero, one, or more optical detection probe binding sites.

Abstract: Methods for capturing a target nucleic acid from a sample by using a capture probe that binds nonspecifically to the target nucleic acid and binds specifically to an immobilized probe via a specific binding pair that has one member on the capture probe and one member on the immobilized probe are disclosed. Compositions that include a capture probe that binds nonspecifically to a target nucleic acid and specifically to an immobilized probe via binding of members of a specific binding pair in a solution phase of a reaction mixture are disclosed.

Abstract: Provided herein are polynucleotides and CRISPR effector proteins configured to be covalently bound together in a CRISPR complex. The polynucleotides can be further modified to modulate the activity of the CRISPR complex. Modification of the polynucleotide and CRISPR effector protein can be used to improve the efficacy of target binding and/or cleavage.

Abstract: The invention, in part, includes compositions comprising quadruplet decoding tRNAs and their encoding sequences. The invention also includes assay methods to assess quadruplet decoding as well as methods of preparing quadruplet decoding suppression tRNAs.

Abstract: The present disclosure relates to modified antisense oligonucleotides. The nucleotides described herein are of 10 to 40 nucleobases and include a targeting sequence complementary to a target region within intron 1 of a pre-mRNA of the human alpha glucosidase (GAA) gene. The target region includes at least one additional nucleobase compared to the targeting sequence, wherein the at least one additional nucleobase has no complementary nucleobase in the targeting sequence, and wherein the at least one additional nucleobase is internal to the target region.

Abstract: Provided are methods of modulating gene expression of a target RNA in a cell comprising (a) recruiting a modulation unit, wherein the modulation unit comprises an RNA binding protein (RBP), an exogenous RNA binding moiety, and a gene-editing agent; (b) delivering the modulation unit into the cell; and (c) detecting change in the target RNA translation, wherein the modulation unit modulates gene expression of the target RNA in the cell.

Abstract: The present invention relates to the fields of medicine and immunology. In particular, it relates to novel antisense oligonucleotides that may be used in the treatment, prevention and/or delay of Leber congenital amaurosis.

Abstract: The present application relates to the field of cancer, particularly that of cancers with high MDM4 protein levels (such as melanoma, breast, colon or lung cancers, glioblastoma, retinoblastoma, etc.). It is shown herein that direct and selective inhibition of MDM4, e.g., by antisense RNA, leads to growth inhibition of cancer cells and sensitization to chemo or targeted therapies. Also provided are simple ways of determining which patients are most amenable for such treatment by comparing specific transcript levels.

Abstract: Therapeutic compounds for red blood cell-mediated delivery of an active pharmaceutical ingredient to a target cell are described. The therapeutic compounds are configured to bind CD47 on the surface of a red blood cell and to be subsequently transferred to CD47 on the surface of the target cell, the therapeutic compound ultimately being internalized by the target cell via endocytosis. The target cell may be a cancer cell.

Abstract: P63 and p73 (and their genes TP53, TP63, TP73) are truncated at or around the tetramerization domain and fused in frame to a heterologous tetramerization domain from a bacterial gene/protein or viral gene/protein or non-human protein lacking homology to any human proteins. Alternatively, the native tetramerization domain can be excised leaving some 3? sequences. These fusion proteins have the activity of the original protein but are unable to hetero-tetramerize or dimerize or be negatively regulated. Further, since non-human tetramerization sequences with minimal homology to human sequences are used, the fusion proteins will not interact with other tetramerization domains in the cell. Gene therapy treatments can thus be used to treat diseases in which these proteins are implicated, such as cancer, autoimmune, and autoinflammatory diseases.

Abstract: Provided is a gene expression cassette which, for the production of a high-quality recombinant protein in bacteria, initiates translation after completion of transcription, and more specifically, to a gene expression cassette consisting of a switch capable of stopping translation initiation and a trigger system capable of activating the translation initiation from the switch by re-configuring the transcription-translation coupled system inherent in bacteria such that the translation is initiated only by a full-length mRNA chain template. The transcription and translation in the bacteria can be uncoupled by inserting a trigger sequence activating the translation initiation from the switch into the downstream (3? terminal) of a target recombinant gene by replacing a natural transcription translation-coupled 5? UTR with the switch. The productivity of a high-quality full-length recombinant protein can be increased while reducing the costs associated with a purification process.

Abstract: Provided is a composition for simultaneously targeting a nucleic acid sequence and providing intron selection in cells in vitro, ex vivo or in vivo. The composition includes one or more nucleic acid molecules each including an artificial nucleic acid sequence flanked with capping sequences, and Lambda beta protein or a linear or circular vector including a nucleic acid sequence encoding the Lambda beta protein, wherein each of the capping sequences is homologous to a region in a target nucleic acid sequence, and the artificial nucleic acid sequence is an intron sequence. The present disclosure also provides a method for editing a target nucleic acid sequence in cells by introducing the composition into the cells.

Abstract: The present invention relates to the field of plant breeding and in particular to the regeneration of plants from cells and other tissues. More particularly, the invention provides methods and means for improving callus and shoot formation and regeneration of plants using hyperphyllin or derivatives thereof.

Abstract: The present invention provides a method for modulating the alkaloid content of a plant (e.g. a tobacco plant), the method comprising modifying said plant by modulating the activity or expression of at least one Nic1 ERF gene. The present invention also provides for the use of at least one Nic1 ERF gene for modulating the alkaloid content of a plant, as well as tobacco cells, plants, plant propagation materials, harvested leaves, processed tobaccos, or tobacco products obtainable in accordance with the invention.

Abstract: The present invention relates to methods of increasing the cold tolerance of a plant or part thereof and/or preventing or inhibiting bolting of a plant, comprising deregulating phloem flux and plants or parts thereof having deregulated phloem flux. The invention also extends to the use of genes for deregulating phloem flux in a plant or part thereof; and/or increasing cold tolerance of a plant or part thereof; and/or preventing or inhibiting bolting in a plant. The invention also provides methods of selecting and/or producing a plant with deregulated phloem flux and/or increased cold tolerance and/or delayed or inhibited bolting. The invention also extends to constructs, isolated polynucleotides and polypeptides which can be used to deregulate phloem flux, plant cells transformed with such constructs, and to plants or parts thereof having deregulated phloem flux.

Abstract: This invention relates to recombinant lentiviral vectors, compositions thereof, the use of the vectors or the compositions thereof, kits of parts comprising said vectors or compositions thereof and a catalytically active Cas9 or Cpf1 protein, methods for modifying the genome of a hematopoietic stem/progenitor cell (HSPC), and the HSPC obtainable by such methods.

Abstract: Genetic circuits have been developed to regulate behaviors of replicon RNA in responses to small molecules, which has broader applications, such as for quantitative expression of cargo genes, temporary expression of immunomodulatory cytokines or antigens for better cancer immunotherapy or vaccination, and for increased safety in use of self-replicating vectors or in combination with other viral-delivery vectors. Described herein are genetic circuits suitable for systems that either require a tight off state or a slow off state, which can serve for instance where either a kill switch or prolonged protein expression (e.g., of vaccine antigens) are needed.

Abstract: This disclosure describes methods for organoid generation including, for example, for generation of a multi-tissue organoid. The multi-tissue organoid may include cartilage, bone, epithelium, and/or fibrous connective tissue. This disclosure further describes methods for isolating cells from the organoids and methods of using the organoids and cells of the organoids.

Abstract: Disclosed herein are neural extracellular vesicles (EVs) and methods of using these EVs in the treatment of spinal cord injury, stroke, and traumatic brain injury and neurodegenerative disease.

Abstract: Disclosed herein are methods of producing platelets and red blood cells using synthetic biology and uses thereof. The methods disclosed herein can also be used to produce platelets and red blood cells comprising a therapeutic agent. The cells produced by the methods disclosed herein can be used to treat, manage, prevent and diagnosis various diseases and disorders and be used as a research tool.

Abstract: Provided is a composition capable of promoting osteogenic differentiation of stem cells, comprising, as active ingredients, a bone and cartilage progenitor cell culture solution and a multilayer graphene film, which promotes the differentiation of stem cells into specific cells, and thus is expected to be variously applicable in the in vivo/in vitro stem cell application fields.

Abstract: This invention relates to methods and materials for nucleic acid delivery, vaccination, and/or treating cancer. More specifically, methods and materials for nucleic acid delivery, vaccination, and/or treating cancer using one or more recombinant adenoviruses (Ads) as an oncolytic agent are provided.

Abstract: Provided herein are engineered variants of archaeal, prokaryotic, and eukaryotic polymerases that exhibit enhanced thermostability, enhanced incorporation of 3? modified nucleotides, and improved uracil-tolerance, in polymerase-catalyzed nucleotide extension reactions relative to wild type polymerase enzymes. Also provided are uses of the engineered polymerases for forming complexed polymerases, forming binding complexes and forming ternary complexes, and uses for conducting nucleic acid sequencing reactions.

Abstract: The present invention provides a 2-OST mutant exhibiting a high activity.

Abstract: A cyclic polypeptide, derivative or analogue thereof, comprising an amino acid sequence derived from the C-terminus of acetylcholinesterase (AChE), or a truncation thereof.

Abstract: The present invention provides a complex containing a nucleic acid sequence-recognizing module and a proteolysis tag, wherein the module is linked to the proteolysis tag, the module specifically binds to a target nucleotide sequence in a double stranded DNA, and the tag consists of (i) a peptide containing 3 hydrophobic amino acid residues at the C-terminal, or (ii) a peptide containing 3 amino acid residues at the C-terminal wherein at least a part of the amino acid residues is substituted by serine.

Abstract: CRISPR RNA-guided nucleases are routinely used for sequence-specific manipulation of DNA. While CRISPR-based DNA editing has become routine, analogous methods for editing RNA have yet to be established. Here we repurpose the type III-A CRISPR RNA-guided nuclease for sequence-specific cleavage of the SARS-CoV-2 genome. The type III cleavage reaction is performed in vitro using purified viral RNA, resulting in sequence-specific excision of 6, 12, 18 or 24 nucleotides. Ligation of the cleavage products is facilitated by a DNA splint that bridges the excision and RNA ligase is used to link the RNA products before transfection into mammalian cells. The SARS-CoV-2 RNA is infectious and standard plaque assays are used to recover viral clones. Collectively, this work demonstrates how type III CRISPR systems can be repurposed for sequence-specific editing of RNA viruses including SARS-CoV-2 and more generally for gene therapy.

Abstract: Novel engineered non-natural deaminases are provided. Methods and compositions are provided for modifying the genome of a cell, including a plant cell. Genome modification through guided CRISPR polypeptides and multiple deaminases are provided. Engineered non-natural novel deaminases, including adenosine deaminases are provided. These novel deaminases have improved activity in cells including plant cells.

Abstract: The present application relates to a variant of ornithine decarboxylase or protein, a polynucleotide encoding the same, a microorganism containing the same, and a method for producing putrescine using the same. The present invention achieves effects of increasing putrescine productivity, production efficiency or production selectivity, suppressing side reactions, and saving the cost involved in purifying putrescine.

Abstract: Compositions and methods for synthesizing an RNA product are provided herein. For example, the present disclosure provides a method of producing an RNA product comprising incubating an in vitro transcription mixture, thereby producing an RNA product that comprises a plurality of single-stranded RNA molecules. In some embodiments, an in vitro transcription mixture comprises a DNA template comprising an RNA polymerase promoter sequence operatively linked to a target sequence; at least one RNA polymerase that recognizes the RNA polymerase promoter sequence; a plurality of ribonucleotides comprising at least two different types of ribonucleotides, each type comprising a different nucleoside; and a transcription buffer comprising an osmolyte.

Abstract: Polypeptides having target levels of C-terminal variants are described.

Abstract: A bacterial strain suitable for expressing recombinant proteins contains an open reading frame which codes for a recombinant protein, under control of a functional promoter. The bacterial strain contains an open reading frame which codes for a muted peptidologycan-associated lipoprotein (PAL protein), under control of a functional promoter, wherein the PAL protein is muted such that it contains no membrane anchor for the outer cell membrane of the bacterial strain. A plasmid codes for a recombinant protein. Fermentative production of recombinant protein is facilitated by using the bacterial strain, with increased product yields in the culture residue without leading to a substantial die-off of the bacterial cells.

Abstract: Provided herein are compositions and methods for producing milk proteins, which allow for safe, sustainable and humane production of milk proteins for commercial use, such as use in food compositions. The disclosure provides recombinant fusion proteins comprising at least first protein and a second protein, wherein at least one of the first protein and the second protein is a milk protein, or fragment thereof. The disclosure also provides methods for producing the recombinant fusions proteins, and food compositions comprising the same.

Abstract: Processes and systems for biologically producing hydrogen gas from organic waste, including food waste. Such a process includes biologically producing hydrogen gas from organic waste by anaerobic fermentation of the organic waste with at least one strain of yeast.

Abstract: A method for producing biogas includes: anaerobically digesting excrement with an additive, the additive including a pre-pupal stage of an insect from the scientific classification superfamily of Stratiomyoidea; and collecting the biogas. A method for producing an additive for anaerobic digestion of excrement includes: hatching eggs of an insect from the scientific classification superfamily of Stratiomyoidea in excrement; growing insect larvae by feeding with additional excrement; harvesting pre-pupal insect larvae; and grinding the pre-pupal larvae. An apparatus for producing an additive for anaerobic digestion of excrement includes: a tray having pivot along one lateral side and an upwardly sloping wall terminating with a lip along a lateral side opposite the pivot; a flume adjacent the lip; and a conveyor adjacent the pivot.

Abstract: The present invention relates to a method for predicting the invasive potential of a tumor cell and predicting the efficiency of a treatment. The method is based on the combination of at least three parameters, namely the internuclear distance, the polarization and the cell-cell connectivity.