Abstract: The present disclosure relates to biocide compositions compatible with enzyme biosensors and methods for using same. More particularly, the disclosure relates to biocide compositions compatible with enzymes used for measuring creatine and creatinine levels.

Abstract: Various apparatus, systems, and methods for measuring a solution characteristic of a sample comprising microorganisms are disclosed. The measured solution characteristic can be used to generate a standardized inoculum. In one embodiment, a sensor apparatus is disclosed comprising a sample container having a chamber lateral wall surrounding a chamber cavity configured receive the sample, a reference sensor fabricated as a container cap and comprising a reference electrode material and, and an active sensor made of a substrate covered in part by an active electrode layer. The active sensor can be coupled to at least part of the chamber lateral wall at a window opening defined along the chamber lateral wall. The solution characteristic can be measured using a reader configured to electrically couple to the sensor apparatus and measure the solution characteristic based on a potential difference between the active sensor and the active sensor.

Abstract: A method of detecting a state of a lipid membrane in a cell of a nanopore based sequencing chip is disclosed. A lipid membrane is coupled with an integrating capacitor, wherein the lipid membrane is between a working electrode and a counter electrode. An alternating current (AC) voltage is applied to the counter electrode. A voltage across the integrating capacitor is periodically sampled by an analog-to-digital converter (ADC). A change in the sampled voltage across the integrating capacitor in response to an intermediate change in the AC voltage is determined. A state of the lipid membrane is determined based on the determined change in the sampled voltage across the integrating capacitor in response to the intermediate change in the AC voltage.

Abstract: The present invention provides methods, compositions and devices for stabilizing the extracellular nucleic acid population in a cell-containing biological sample and for stabilizing the transcriptome of contained cells.

Abstract: A microfluidic device includes an input port for inputting a particle-containing liquidic samples into the device, a retention member, and a pressure actuator. The retention member is in communication with the input port and is configured to spatially separate particles of the particle-containing liquidic sample from a first portion of the liquid of the particle containing fluidic sample. The pressure actuator recombines at least some of the separated particles with a subset of the first portion of the liquid separated from the particles. The device can also include a lysing chamber that receives the particles and liquid from the retention member. The lysing chamber thermally lyses the particles to release contents thereof.

Abstract: The present invention relates to a method of isolating a nucleic acid target region from a population of nucleic acid molecules, said method comprising the steps of a) contacting said population of nucleic acid molecules with a Class 2 Type V Cas protein-gRNA complex, wherein the gRNA comprises a guide segment that is complementary to a first site adjacent to said target region, thereby forming a Class 2 Type V Cas protein-gRNA-nucleic acid complex, b) contacting the population of nucleic acid molecules comprising said Class 2 Type V Cas protein-gRNA-nucleic acid complex with at least one enzyme having single-strand 3? to 5? exonuclease activity, thereby forming a 5? single-stranded overhang in said first site, c) removing the Class 2 Type V Cas protein-gRNA complex from the population of step b), d) contacting the population of step c) with an oligonucleotide probe, said probe comprising a sequence that is at least partially complementary to said overhang, thereby forming a duplex between said probe and said

Abstract: Provided are a gene detection kit and a gene detection device. The gene detection kit includes a kit body, a piston cylinder, and a piston. The kit body has an accommodating cavity and a plurality of reagent cavities. The piston cylinder is provided in the accommodating cavity, and the piston cylinder has a piston cavity. The piston is movably provided in the piston cavity along an axial direction of the piston cylinder. A first channel in communication with the piston cavity is provided on an outer circumferential surface of the piston cylinder, a plurality of second channels are provided on an inner wall of the accommodating cavity, each of the second channels is in corresponding communication with one of the reagent cavities, and the piston cylinder can move relative to the kit body, so that the plurality of second channels are alternately in communication with the first channel.

Abstract: Disclosed are compositions and methods for the multiplexed analysis of one or more intracellular targets of a single cell. Exemplary compositions of the disclosure comprise a surface comprising a plurality of capture agents operatively-linked thereto, wherein each capture agent specifically binds to a distinct intracellular target and wherein the plurality of capture agents form a repeating pattern; a substrate comprising a plurality of chambers, wherein the substrate releasably couples with the surface and wherein each chamber of the plurality of chambers comprises at least one repeat of the repeating pattern of the plurality of capture agents of the surface; a coating composition comprising a cell lysis composition; and a linker composition comprising a functionalization component and an extension component.

Abstract: Methods and apparatus for direct detection of chemical reactions are provided. Electric charge perturbations of the local environment during enzyme-catalyzed reactions are sensed by an electrode system with an immobilized target molecule. The charge perturbation caused by the polymerase reaction can uniquely identify a DNA sequence. The polymerization process generates local perturbations of charge in the solution near the electrode surface and induces a charge in a polarazible gold electrode. This event is detected as a transient current by a voltage clamp amplifier. Detection of single nucleotides in a sequence can be determined by dispensing individual dNTPs to the electrode solution and detecting the charge perturbations. Alternatively, multiple bases can be determined at the same time using a mix of all dNTPs with subsequent analysis of the resulting signal.

Abstract: Methods and systems described herein involve using long cell-free DNA fragments to analyze a biological sample from a pregnant subject. The status of methylated CpG sites and single nucleotide polymorphisms (SNPs) is often used to analyze DNA fragments of a biological sample. A CpG site and a SNP are typically separated from the nearest CpG site or SNP by hundreds or thousands of base pairs. Finding two or more consecutive CpG sites or SNPs on most cell-free DNA fragments is improbable or impossible. Cell-free DNA fragments longer than 600 bp may include multiple CpG sites and/or SNPs. The presence of multiple CpG sites and/or SNPs on long cell-free DNA fragments may allow for analysis than with short cell-free DNA fragments alone. The long cell-free DNA fragments can be used to identify a tissue of origin and/or to provide information on a fetus in a pregnant female.

Abstract: The present disclosure relates in some aspects to methods and compositions for analysis of a target nucleic acid, such as in situ detection of a region of interest in a polynucleotide in a tissue sample. In some embodiments, provided herein are templated ligation probes (e.g., RNA-templated ligation probes) and selector probes for generation of a circularized ligated probe comprising an insertion sequence of a selector probe, wherein the circularized ligated probe is amplified in a rolling circle amplification reaction to generate a product that is detected in the sample.

Abstract: This invention provides methods for detecting RNAs in a biological sample while using only a small amount of sample input, e.g., less than or equal to 1 mL. The methods described herein are able to detect a large percentage of protein-coding genes having the ENSEMBL gene annotation HG38.

Abstract: A method for detecting a gene fusion includes amplifying a nucleic acid sample in the presence of primer pool to produce a plurality of amplicons. The primer pool includes primers targeting a plurality of exon-exon junctions of a driver gene. The amplicons correspond to the exon-exon junctions. The amplicons are sequenced and aligned to a reference sequence. The number of reads corresponding to each amplicon is normalized to give a normalized read count. A baseline correction is applied to the normalized read counts for the amplicons to form corrected read counts. A binary segmentation score is calculated for each corrected read count. A predicted breakpoint for the gene fusion is determined based on the amplicon index corresponding to the maximum absolute binary segmentation score. Gene fusion events may be detected in a partner agnostic manner, i.e. without prior knowledge of the specific fusion partner genes or specific breakpoint information.

Abstract: Provided herein are methods of making, amplifying, and sequencing tagged nucleic acid complements, compositions including interposing oligonucleotide barcodes, and kits useful in obtaining long-range sequence data.

Abstract: The invention relates to methods of RNA amplification, including methods for the reverse transcription of cDNA from RNA using a thermostable reverse transcriptase. In a particular aspect, the methods are capable of linear amplification of an RNA template through multiple cycles of cDNA synthesis.

Abstract: Provided herein are methods, compositions, and systems for multiplexed analysis of individual cells or cell populations. Cells encapsulated in beads and/or biomolecules are sequentially co-partitioned, allowing for analysis of two different types of biomolecules (e.g., RNA and DNA). The present invention leverages different polymer dissociation mechanisms, accompanied with barcoding of biomolecules (e.g., nucleic acid molecules) for multiplexed measurements in single cells. Sequential co-partitioning and barcode technology enables identification and quantitation of DNA and RNA from single cells.

Abstract: DNA is sequenced by (a) independently sequencing first and second strands of a dsDNA to obtain corresponding first and second sequences; and (b) combining the first and second sequences to generate a consensus sequence of the dsDNA. By independently sequencing first and second strands the error probability of the consensus sequence approximates a multiplication of those of the first and second sequences.

Abstract: A sensor apparatus includes a substrate, a semiconductor device disposed over the substrate, the semiconductor device having a surface electrode structure, and a saccharide coating formed over the surface electrode structure. The saccharide coating can be removed prior to use. The semiconductor device can further include a well and optionally a bead disposed in the well.

Abstract: Disclosed herein, inter alia, are methods of detecting or sequencing circular template polynucleotides on a solid support and/or in cells and tissues.

Abstract: The invention provides compositions and methods for determining the fraction of fetal nucleic acids in a maternal sample comprising a mixture of fetal and maternal nucleic acids. The fraction of fetal nucleic acids can be used in determining the presence or absence of fetal aneuploidy.

Abstract: The invention relates to an in-vitro method for identifying a diabetic patient as responder or non-responder to a treatment of Proinsulin, Interleukin 10 and anti-CD3, based on the expression level of at least 1 microRNA, selected from the group consisting of miR-365, miR-34a, miR-193b and miR-125a, in a sample from the diabetic patient prior to said treatment.

Abstract: Compositions and methods for the detection and treatment of ADHD are provided.

Abstract: Methods and compositions are provided for determining a subtype of lung adenocarcinoma (AD) of an individual by detecting the expression level of at least one classifier biomarker selected from a group of gene signatures for lung adenocarcinoma. Also provided herein are methods and compositions for determining the response of an individual with an adenocarcinoma subtype to a therapy such as immunotherapy.

Abstract: Provided herein are compositions and methods for characterizing and treating cancer. In particular, provided herein are compositions and methods for identifying subjects with breast cancer with increased likelihood of bone metastasis and providing treatment to prevent such metastases.

Abstract: Methods and compositions are provided for determining a subtype of lung squamous cell carcinoma (SQ) of an individual by detecting the expression level of at least one classifier biomarker selected from a group of gene signatures for lung squamous cell carcinoma. Also provided herein are methods and compositions for determining the response of an individual with a squamous cell carcinoma subtype to a therapy such as immunotherapy.

Abstract: The present invention relates to refined prognostic clinical tools, methods, and kits for the evaluation of risk and treatment of distant recurrence in ER+/HER2? breast cancer patients.

Abstract: A correlative finding between increased Clostridiales and/or Bifidobacteriales bacterial abundance in the gut and intestinal barrier maturation of preterm neonates at-risk for development of necrotizing enterocolitis (NEC) is disclosed. These findings form the basis for the methods of identifying preterm infants at risk for developing necrotizing enterocolitis (NEC), the methods of treating such infants, as well as the methods of characterizing intestinal permeability in preterm infants disclosed herein.

Abstract: The present invention relates to novel primers and sloppy molecular beacon and molecular beacon probes for amplifying segments from different genes in Mycobacterium tuberculosis for identifying the presence of M.tb DNA and/or resistance to anti-tuberculosis drugs.

Abstract: A method of diagnosing bacterial vaginosis in a woman, which involves determining an amount of each of more than one BV-associated bacterium in a vaginal sample obtained from the female and assessing a BV status of the female based on the amount of each of the more than one BV-associated bacterium in the sample.

Abstract: The present disclosure provides watermelon plants with a male sterile phenotype and their progeny. Such plants may comprise an introgressed genomic region associated with a male sterile phenotype. In certain aspects, compositions, including distinct polymorphic molecular markers and methods for producing, breeding, identifying, and selecting plants or germplasm with a male sterile phenotype are provided.

Abstract: A high-strength steel sheet that has a predetermined component composition, that has a steel microstructure in which an area percentage of ferrite ranges from 5% to 50% in a thickness cross-section in a rolling direction, a total area percentage of fresh martensite and retained ? ranges from 2% to 30%, each of the fresh martensite and the retained ? has an average grain size of 5 ?m or less, and a ratio of the fresh martensite and the retained ? adjacent only to ferrite with respect to the fresh martensite and the retained ? from a surface to 200 ?m in the thickness direction is 30% or less in total area percentage, and that has a yield strength of 550 MPa or more.

Abstract: A high strength steel sheet according to the present invention contains a predetermined chemical composition, in a metallographic structure, the total area ratio of tempered martensite and bainite is 80% or more, at a sheet thickness ¼ position of a cross section parallel to a rolling direction and perpendicular to a rolled surface, the standard deviation of number densities of precipitates having a diameter of 10 nm or less and including one or both of Ti and Nb is less than 5×1010 numbers/mm3, and the tensile strength is 780 MPa or more.

Abstract: A method for producing ultra-thin hot-rolled strip steel, the method comprising the following process steps: A. a smelting process: feeding scrap steel into an induction electric furnace (1) for smelting so that the scrap steel melts into molten steel; B. a refining process: using a ladle refining furnace (2) and a ladle vacuum degassing furnace (3) to refine the molten steel; C. a continuous casting process: casting the refined molten steel into a cast strip blank that has a thickness of 1.6-2.5 mm by means of a dual-roller thin strip continuous casting system (4); D. a hot rolling process: directly feeding the cast strip blank that was cast in the continuous casting process to a single-stand hot rolling mill (9) for rolling to produce hot-rolled strip steel, the thickness of the hot-rolled strip steel being 0.8-1.5 mm; E. a cooling coiling process: performing atomizing cooling on the hot-rolled strip steel, and coiling after the strip steel temperature is controlled to be 400-750° C.

Abstract: A suspension roasting system includes a feeding bin, a Venturi dryer, a first cyclone preheater, a second cyclone preheater, a pre-oxidation suspension roasting furnace, a thermal separation cyclone cylinder, a suspension and reduction roasting furnace, a collecting bin, a grinding machine, a magnetic ore separator and a draught fan.

Abstract: The present invention relates to a process for purifying and concentrating rare earths contained in phosphogypsum, characterised in that it comprises the following steps of: from a phosphogypsum, a) Leaching the phosphogypsum with a solution of one or more strong acid(s) selected from among: sulphuric acid, nitric acid and hydrochloric acid, in order to obtain a leaching mixture comprising a liquid phase formed by a leaching solution containing rare earths from the phosphogypsum and the leaching acid, and a solid phase comprising the phosphogypsum, b) Adding, to the phosphogypsum, an oxidising agent to promote passage of the rare earths from the phosphogypsum into the leaching solution, and/or a reducing agent to reduce solubility of mineral impurities contained in the leaching solution in order to allow their passage from the leaching solution into the solid phase, c) Separating the liquid phase enriched in rare earths and depleted in mineral impurities, and the solid phase enriched in mineral impurities.

Abstract: A heat-resistant alloy contains Y, a content of Ni is a content of Ni is 58.0 mass % or greater, a content of Cr is 20.0 mass % or greater and 23.0 mass % or less, a content of Mo is 8.0 mass % or greater and 10.0 mass % or less, a content of Nb is 3.15 mass % or greater and 4.15 mass % or less, a content of O is 0.002 mass % or greater and 0.1 mass % or less, and a ratio of a content of Y in terms of mass to a content of O in terms of mass is 0.5 or greater and 100 or less.

Abstract: An aluminum alloy foil includes: aluminum; silicon; manganese; iron; zinc; copper; and magnesium. In the aluminum alloy foil, a total of a content of the silicon and a content of the iron is less than or equal to 0.1 mass %. In the aluminum alloy foil, a ratio of a mass of the manganese to a total mass of the silicon and the iron is more than or equal to 7.0. In the first surface, an area ratio of second phase particles each having an equivalent circle diameter of more than or equal to 1.5 ?m is less than or equal to 0.1%.

Abstract: An alloy formed of aluminum, nickel, scandium and optionally one, two or more further metals. The aluminum alloy is suitable for additive manufacturing of lightweight highly thermally conductive components for aircraft, such as heat exchangers. In a first step, a powder of the described aluminum alloy is produced by additive manufacturing, such as laser melting in the L-PBF process. Large grains are able to grow epitaxially along the build direction thereby increasing phonon and electron mobility along the build direction. With this, a higher thermal conductivity can be achieved. In a second step, the preliminary part is hardened by precipitation of secondary phases at 250 to 400 C to form the hardened part. 3D-printed lightweight parts with high thermal conductivity are obtained.

Abstract: Provided is a steel sheet by weight percentage (wt %), carbon (C): 0.005 to 0.08%, manganese (Mn): 1.25% or less (excluding 0%), phosphorus (P): 0.03% or less (excluding 0%), sulfur (S): 0.01% or less (excluding 0%), nitrogen (N): 0.01% or less (excluding 0%), soluble aluminum (sol.Al): 0.01 to 0.06%, chromium (Cr): 1.15 to 2.5%, antimony (Sb): 0.1% or less (excluding 0%), at least one selected from the group consisting of nickel (Ni): 0.3% or less (excluding 0%), silicon (Si): 0.3% or less (excluding 0%), molybdenum (Mo): 0.2% or less (excluding 0%), and boron (B): 0.003% or less (excluding 0%), and a remainder of iron (Fe) and other unavoidable impurities, satisfying Expression 1: 1.3?Mn(wt %)+Cr(wt %)/1.5+Sb(wt %)?2.7, where Mn, Cr, and Sb refer to contents (wt %) of corresponding elements, respectively; and 1 to 5% of martensite and a remainder of ferrite by an area percentage (area %).

Abstract: Provided are a hot-rolled steel sheet for an electric resistance welded steel pipe and a method for manufacturing the same, an electric resistance welded steel pipe and a method for manufacturing the same, a line pipe, and a building structure. The hot-rolled steel sheet has a chemical composition containing, by mass %, C: 0.030% or more and 0.20% or less, Si: 0.02% or more and 1.0% or less, Mn: 0.40% or more and 3.0% or less, P: 0.050% or less, S: 0.020% or less, N: 0.0070% or more and 0.10% or less, and Al: 0.005% or more and 0.080% or less, the balance being Fe and incidental impurities, in which N dissolved in steel is contained in an amount of 0.0010% or more and 0.090% or less, and letting a sheet thickness be t, a steel microstructure at a ½t position has an average grain size of 20.0 ?m or less.

Abstract: A non-oriented electrical steel sheet according to an embodiment of the present invention includes: in wt %, C at 0.004 wt % or less (excluding 0 wt %), Si at 2.5 to 4.0 wt %, P at 0.1 wt % or less (excluding 0 wt %), Al at 0.3 to 2.0 wt %, N at 0.003 wt % or less (excluding 0 wt %), S at 0.003 wt % or less (excluding 0 wt %), Mn at 0.15 to 2.5 wt %, Cr at 0.5 wt % (excluding 0 wt %), and the balance including Fe and other impurities unavoidably added thereto; satisfies the following Equation 1; and has an average grain size of 20 ?m or less. [Mn]?1450×[S]?0.8??[Equation 1] (In Equation 1, [Mn] and [S] represent a content (wt %) of Mn and S, respectively.).

Abstract: The present invention relates to a method for the manufacture of a coated steel sheet comprising the following successive steps: A. the coating of the steel sheet with a first coating consisting of nickel and having a thickness between 600 nm and 1400 nm, the steel sheet having the following composition in weight: 0.10<C<0.40%, 1.5<Mn<3.0%, 0.7<Si<3.0%, 0.05<Al<1.0%, 0.75<(Si+Al)<3.0%, and on a purely optional basis, one or more elements such as Nb?0.5%, B?0.010%, Cr?1.0%, Mo?0.50%, Ni?1.0%, Ti?0.5%, the remainder of the composition making up of iron and inevitable impurities resulting from the elaboration, B. the recrystallization annealing at a temperature between 820 to 1200° C., C. the coating with a second coating based on zinc not comprising nickel.

Abstract: A process for producing a copper-beryllium alloy product. The process comprises preparing a base alloy having 0.15 wt %-4.0 wt % beryllium and having grains and an initial cross section area. The process further comprises cold working the base alloy to a percentage of cold reduction of area (CRA) greater than 40%, based on the initial cross section area, and heat treating the cold worked alloy to produce the copper-beryllium alloy product. The grain structure of the copper-beryllium alloy product has an orientation angle of less than 45° when viewed along the direction of the cold working. The copper-beryllium alloy product demonstrates a fatigue strength of at least 385 MPa after 106 cycles of testing.

Abstract: A vacuum deposition facility for continuously depositing, on a running substrate, coatings formed from metal or metal alloy, and including: a central casing including a vapor jet coater, the inner walls of the central casing being suited to be heated at a temperature above the condensation temperature of the metal or metal alloy vapors, a vapor trap located at the substrate exit of the central casing, the inner walls of the vapor trap being suited to be maintained at a temperature below the condensation temperature of the metal or metal alloy vapors, the passage linking the central casing to the vapor trap including at least one thermal connector extending at least from the inner walls of the central casing to the inner walls of the vapor trap.

Abstract: The present invention relates to a method of forming a coating layer having plasma resistance, the method comprising steps of: preparing a substrate by placing the substrate in a substrate fixing device inside a process chamber; evaporating a Y2O3 deposition material provided in a solid form in an electron beam source by irradiating an electron beam on the Y2O3 deposition material; generating radical particles having activation energy by injecting a process gas containing oxygen for forming radicals into a RF energy beam source; irradiating an RF energy beam including the radical particles generated in the RF energy beam source, toward the substrate; depositing a thin film in which the evaporated deposition material is deposited on the substrate by being assisted by the RF energy beam, and densifying the thin film in which the deposition material deposited on the substrate forms a densified film by ion bombardment of the RF energy beam.

Abstract: A method of coating a component includes attaching the component to a support that is configured to hold a plurality of components and placing a base of the support in a holder that is attached to rotatable member of a fixture, wherein an axis of the holder is parallel to an axis of rotation of the rotatable member. The method also includes transporting the fixture into a coating chamber wherein a direction of an exit stream of a coater in oriented perpendicularly to the axis of rotation, exposing the fixture and the component to a reverse transfer arc cleaning/pre-heating procedure, and exposing the fixture and the component to a coating procedure during which a coating is directed at the component in a direction perpendicular to the axis of rotation while the rotatable member is rotating. The method further includes transporting the fixture and removing the component from the support fixture.

Abstract: There is provided a technique that includes a reaction vessel including a first region in which a substrate is arranged and a second region in which no substrate is arranged; a heater configured to heat the first region; a gas supplier configured to supply a plurality of gases including cleaning gases; and a controller that controls at least one selected from the group of the gas supplier, the heater, and a cooler to clean the first region and the second region under different conditions by at least one method selected from the group of a method in which the gas supplier supplies two different kinds of cleaning gases, a method in which the gas supplier supplies one or more cleaning gases from two different locations, and a method in which the heater sets a temperature differently between the first region and the second region.

Abstract: The object of the present invention is to provide a cleaning apparatus for a component of a semiconductor production apparatus, which is capable of preventing the attachment of reaction products into the cleaning processing furnace by a simple structure, the present invention provides a cleaning apparatus (1) for a component of a semiconductor production apparatus including: a cleaning processing furnace (2) which is configured to house the component (10) of a semiconductor production apparatus; a heating device (3); a gas introduction pipe (4); a gas discharge pipe (5); a decompression device (6); a first temperature control device (7); a second temperature control device (8); and a purge gas supply device (9).

Abstract: Disclosed is a process stop loss reduction system, in which in case that pressure in a trapping apparatus and pressure in a process chamber are increased because of space clogging or the like caused by reaction by-products while the trapping apparatus for trapping of a reaction by-product contained in exhaust gas discharged from the process chamber operates over a long period of time during a semiconductor process, only the trapping apparatus, in which an exhaust gas supply flow path is blocked, may be quickly replaced while inert gas, instead of the exhaust gas, is received in an idle state and continuously supplied to the vacuum pump through a bypass pipe without stopping an operation of (shutting down) a semiconductor manufacturing process chamber facility, and then the trapping apparatus may be supplied with the exhaust gas again as the flow path is changed.

Abstract: Embodiments described herein generally relate to a processing system and a method of delivering a reactant gas. The processing system includes a substrate support system, an injection cone, and an intake. The injection cone includes a linear rudder. The linear rudder is disposed such that the flow of reactant gas through the injection cone results in film growth on a specific portion of a substrate. The method includes flowing the gas through the injection cone and delivering the gas onto the substrate below. The localization of the reactant gas, allows for film growth on a specific portion of the substrate.