Abstract: The present disclosure relates to developing an enriched steady state microbial consortium capable of modifying crude oil components of an oil reservoir under anaerobic denitrifying conditions. The steady state consortium may be used to improve oil recovery.

Abstract: The present invention is generally directed to a method for releasing trapped oil in reservoirs. The method hereof includes identifying a reservoir, obtaining a microbial community sample of the reservoir, maintaining the sample under high temperature and high pressure conditions that mimic natural conditions of the reservoir, growing the sample on at least one substrate, determining a targeted treatment regime for the reservoir based on the positive growth of the sample on the substrate, injecting the reservoir with the targeted substrate to release trapped oil in the reservoir, monitoring the reservoir, and extracting the oil from the reservoir.

Abstract: The method hereof is for releasing trapped oil in reservoirs and includes identifying a reservoir, obtaining a microbial community sample of the reservoir, maintaining the sample under high temperature and high pressure conditions that mimic natural conditions of the reservoir, growing the sample on at least one substrate, determining a targeted treatment regime for the reservoir based on the positive growth of the sample on the substrate, injecting the reservoir with the targeted substrate to release trapped oil in the reservoir, monitoring the reservoir, and extracting the oil from the reservoir.

Abstract: Embodiments of the invention described herein relate to methods and apparatus for recovery of viscous hydrocarbons from subterranean reservoirs. In one embodiment, a method for recovery of hydrocarbons from a subterranean reservoir is provided. The method includes drilling an injector well to be in communication with a reservoir having one or more production wells in communication with the reservoir, installing casing in the injector well, cementing the casing, perforating the casing, positioning a downhole steam generator in the casing, flowing fuel, oxidant and water to the downhole steam generator to intermittently produce a combustion product and/or a vaporization product in the reservoir, flowing injectants to the reservoir, and producing hydrocarbons through the one or more production wells.

Abstract: Methods to enhance biogenic gas production in an anaerobic geologic formation containing carbonaceous material may include the steps of accessing the anaerobic formation, increasing a rate of production of the biogenic gases in the anaerobic formation, and flowing formation water within the anaerobic formation after the increase in the production of biogenic gases. Methods to redistribute formation water in an anaerobic geologic formation containing carbonaceous material may include the steps of locating a reservoir of the formation water within the anaerobic formation, forming at least one channel between the reservoir and at least a portion of the carbonaceous material, and transporting the formation water from the reservoir to the carbonaceous material through the channel. The methods may also include the accumulation of biogenic gas in an anaerobic geologic formation to enhance biogenic gas production.

Abstract: Methods are provided for treating production fluid in a production well in an oil reservoir to reduce the amount of sulfide in the production fluid. The production fluid is treated with nitrate and/or nitrite ions or inorganic oxidizing agent in an aqueous solution that is added to the well casing.

Abstract: Methods for enriching microbial populations that are indigenous to oil reservoir fluids to obtain Shewanella-enriched populations and isolated Shewanella strains useful for enhancing oil recovery from an oil reservoir are presented. In addition, the enriched populations may contain bacteria belonging to the Arcobacter genus.

Abstract: A post cold heavy oil production with sand (“CHOPS”) microbial enhanced oil recovery method comprises selecting a well having at least one wormhole and that has completed primary CHOPS production. When the reservoir does not contain enough of a gas producing indigenous microbe, an injectant is prepared comprising a gas-producing microbe, a nutrient suitable for the microbe, and a fluid base. The injectant is injected through the well and into the at least one wormhole in the reservoir; the well is shut in until pressure in the well reaches the target pressure; and then the well is produced.

Abstract: The invention relates to treating of subterranean formations to increase the susceptibility (by way of chemical break down or solubilization, but not necessarily limited by these or any other theories) of large carbonaceous molecules therein, such as comprise coal, to bioconversion into methane and other useful hydrocarbon products by indigenous and/or non-indigenous microbial consortia, by introducing into the subterranean formations: (a) a solution containing at least one of an oxoacid ester of phosphorus or a thioacid ester of phosphorus; and (b) one or more other chemical compounds/chemical entities selected from the group consisting of: hydrogen, carboxylic acids, esters of carboxylic acids, salts of carboxylic acids, oxoacids of phosphorus, salts of oxoacids of phosphorus, vitamins, minerals, mineral salts, metals, and yeast extracts.

Abstract: A process for producing methane, carbon dioxide, gaseous and liquid hydrocarbons, and other valuable products from subterranean carbon bearing formations, in-situ. The process utilizes indigenous and/or non-indigenous microbial consortia that are capable of converting a carbon-bearing material such as shale or coal to desired products. The process comprises injecting fluid into a carbon bearing deposit with at least one injection well and removing injected fluid and product from the deposit through at least one production well, and controlling fluid pressure within at least a portion of the deposit by use of the injected fluid, the pressure being controlled such that the fluid pressure within at least a portion of the deposit exceeds the fluid pressure that normally exists in that portion.

Abstract: The present invention describes methods of identifying stimulants for the biogenic production of methane in hydrocarbon-bearing formations. Methods involve the use of microbial nucleic acid sequence information for the determination of gene products that are enzymes in a variety of pathways involved in the conversion of hydrocarbons to methane. Enzymes and stimulants identified by invention methods can be used in processes for enhancing biogenic methane production, for example, by addition to coal seams and coalbed methane wells.

Abstract: This invention generally relates to natural gas and methylotrophic energy generation, bio-generated fuels and microbiology. In alternative embodiments, the invention provides nutrient amendments and microbial compositions, e.g., consortia, that are both specifically optimized to stimulate methanogenesis, or for “methylotrophic” or other conversions. In alternative embodiments, the invention provides methods to develop nutrient amendments and microbial compositions that are both specifically optimized to stimulate methanogenesis in a given reservoir. The invention also provides methods for the evaluation of potentially damaging biomass formation and scale precipitation resulting from the addition of nutrient amendments. In other embodiments, the invention provides methods for simulating biogas in sub-surface conditions using a computational model.

Abstract: The use of coal fields as subsurface bioreactors for producing sustainable methane gas from terrestrial sources of biomass is described. Microbial presence is determined for a target coal formation, and tracers are injected to determine permeability, porosity, volume, and minimum and a maximum material injection rates. At least one injection well and at least one circulation well effective for generating an injection rate between the minimum and maximum injection rates are provided for injecting a solution of biodegradable materials into the coal seam. A chosen quantity of biodegradable materials is allowed to be digested, fermented and converted by microbial action within the coal seam. Methane gas is extracted through producing and injecting wells, although pumping will enhance gas recovery.

Abstract: A novel method reduces methane slack when operating a biogas fermenter. When starting up a freshly charged fermenter, the methane portion of the produced biogas is initially so low and the portions of carbon dioxide and nitrogen are so high that the biogas cannot be directly used in a combined heat and power plant. Conventionally, the biogas generated during the startup phase has a small portion of methane that is discharged directly into the atmosphere or is flared off if the methane fraction is larger. The initially produced methane is consequently not used and becomes methane slack. To reduce methane slack, the biogas with the low methane fraction is fed to a gas treatment unit in which non-methane components of the gas mixture are partially separated, and the remaining gas mixture with a higher methane content is returned to the biogas fermenter until the methane fraction is sufficiently high.

Abstract: A method to improve the effectiveness of MEOR or bioremediation processes. In this method toxic chemicals accumulated in subterranean sites adjacent to the water injection wells are either dispersed or removed prior to introduction of microbial inocula for enhanced microbial oil recovery or bioremediation of these sites.

Abstract: Methods and compositions are provided to enhance oil recovery wherein the indigenous microbial population of an oil reservoir is fed a composition containing glutamate and an electron acceptor. The effect of the glutamate carbon source is to promote bioplugging of a permeable environment by the indigenous microorganisms. Bioplugging in an oil reservoir will improve sweep efficiency thereby leading to enhanced secondary oil recovery.

Abstract: A method to improve the effectiveness of MEOR or bioremediation processes. In this method toxic chemicals accumulated in subterranean sites adjacent to the water injection wells are either dispersed or removed prior to introduction of microbial inocula for enhanced microbial oil recovery or bioremediation of these sites.

Abstract: A method to improve the effectiveness of MEOR or bioremediation processes. In this method toxic chemicals accumulated in subterranean sites adjacent to the water injection wells are either dispersed or removed prior to introduction of microbial inocula for enhanced microbial oil recovery or bioremediation of these sites.

Abstract: A method to improve the effectiveness of MEOR or bioremediation processes. In this method toxic chemicals accumulated in subterranean sites adjacent to the water injection wells are either dispersed or removed prior to introduction of microbial inocula for enhanced microbial oil recovery or bioremediation of these sites.

Abstract: A method to improve the effectiveness of MEOR or bioremediation processes. In this method toxic chemicals accumulated in subterranean sites adjacent to the water injection wells are either dispersed or removed prior to introduction of microbial inocula for enhanced microbial oil recovery or bioremediation of these sites.

Abstract: The present invention is related to the field of microbial enhanced oil recovery (MEOR). In particular, the invention concerns new, efficient, economical and environmentally safe microbial methods to enhance oil recovery, as well as microorganisms useful in such methods.

Abstract: A method to improve the effectiveness of MEOR or bioremediation processes. In this method toxic chemicals accumulated in subterranean sites adjacent to the water injection wells are either dispersed or removed prior to introduction of microbial inocula for enhanced microbial oil recovery or bioremediation of these sites.

Abstract: A method to improve the effectiveness of MEOR or bioremediation processes. In this method toxic chemicals accumulated in subterranean sites adjacent to the water injection wells are either dispersed or removed prior to introduction of microbial inocula for enhanced microbial oil recovery or bioremediation of these sites.

Abstract: A method to improve the effectiveness of MEOR or bioremediation processes. In this method toxic chemicals accumulated in subterranean sites adjacent to the water injection wells are either dispersed or removed prior to introduction of microbial inocula for enhanced microbial oil recovery or bioremediation of these sites.

Abstract: In or near real-time monitoring of fluids can take place using an opticoanalytical device that is configured for monitoring the fluid. Fluids can be monitored prior to or during their introduction into a subterranean formation using the opticoanalytical devices. Produced fluids from a subterranean formation can be monitored in a like manner. Specifically, bacteria can be monitored in fluids using opticoanalytical devices. The methods can comprise exposing water to a bactericidal treatment, and after exposing the water to the bactericidal treatment, monitoring live bacteria in the water using an opticoanalyfical device that is in optical communication with the water. Optionally, the water can be introduced into a subterranean formation.

Abstract: Methods for increasing the rate of methane production in a subsurface reservoir are provided.

Abstract: A method of microbial enhanced oil recovery from a wellbore in an oil-bearing formation that includes injecting water into the oil-bearing formation via a tubing string in the wellbore and introducing oxygen into the oil-bearing formation for consumption by microbes in the oil-bearing formation. The oxygen introduction includes delivering the oxygen into the oil-bearing formation without having the introduced oxygen contact the walls of the tubing string and without having the introduced oxygen contact the injected water within the tubing string while the injected water is in the tubing string.

Abstract: A method for environmental monitoring and bioprospecting includes the steps of: (a) utilizing a testing device having: (i) a container having a fluid inlet and outlet, (ii) a plurality of capillary microcosms situated within the container, each of these capillaries having an inlet and outlet that are configured so as to allow for fluid flow through the capillaries, each of these capillaries further having a means for covering its inlet and outlet so as to prevent flow through the capillary, (iii) a pump connected to the container outlet, the pump being configured so as to draw fluid from the surrounding environment into the container's inlet and through the capillaries, (iv) connected to the outlet of the container, a means for collecting the flow through the container, and (v) a check valve connected downstream of the container to prevent the backflow of fluid into the container, (b) adding specified test substances to the device's capillaries, wherein these substances are to be analyzed for their ability to

Abstract: The present invention is generally directed to a method for releasing trapped oil in reservoirs. The method hereof includes identifying a reservoir, obtaining a microbial community sample of the reservoir, maintaining the sample under high temperature and high pressure conditions that mimic natural conditions of the reservoir, growing the sample on at least one substrate, determining a targeted treatment regime for the reservoir based on the positive growth of the sample on the substrate, injecting the reservoir with the targeted substrate to release trapped oil in the reservoir, monitoring the reservoir, and extracting the oil from the reservoir.

Abstract: Efficient, economical and environmentally safe microbial methods to enhance oil recovery in existing oil reservoirs, as well as microorganisms useful in such methods. The microorganisms are capable of growing in an environment of high salinity, and are deficient in their ability to degrade short chain hydrocarbons of about 12 carbons or less.

Abstract: Chemical compounds that are N-lauroyl amino acids or derivatives thereof were found to have oil-releasing activity. Solutions containing these compounds and microorganisms having oil release and/or plugging biofilm formation properties may be introduced into oil reservoirs to improve oil recovery.

Abstract: A method for reducing the deleterious effects of sulfate reducing bacteria in aqueous environments, particularly those in which metal materials are exposed to the microorganisms. A treatment solution containing rotenone is introduced into the aqueous environment. The rotenone is present in the treatment fluid in an amount which is sufficient to inhibit the growth of sulfate reducing bacteria on the metal material.

Abstract: A method of increasing biogenic production of a combustible gas from a subterranean geologic formation is described. The method may include extracting formation water from the geologic formation, where the extracted formation water includes at least a first species and a second species of microorganism. The method may also include analyzing the extracted formation water to identify the first species of microorganism that promotes the biogenic production of the combustible gas. An amendment may be introduced to the formation water to promote the growth of the first species of microorganism, and the biological characteristics of the formation water may be altered to decrease a population of the second species in the geologic formation.

Abstract: Disclosed herein is introduction of different nutrient solution formulations to a subterranean target site to prevent biofilm formation and biomass aggregation at the sand face of an injection well bore during MEOR or bioremediation processes. A first nutrient solution formulation supports growth of introduced microorganisms and a second nutrient solution formulation is used to promote biofilm formation to allow plugging of pores and channels in the subterranean target site, for MEOR or bioremediation.

Abstract: A method of microbial enhanced oil recovery from an oil-bearing formation that involves treating the water that is to be injected into the oil-bearing formation to enable microbial activity and adding oxygen to aid microbial activity. The treatment applied to the water is based, at least in part, upon establishing at least one condition in the oil-bearing formation favorable to microbial activity that enhances movement of oil from the oil-bearing formation.

Abstract: The present invention provides methods for stimulating or enhancing the conversion of coal to methane. The methods of the current invention subject coal to an oxidation step, for example ozonolysis, thereby converting at least a portion of the coal to partially-oxidized and water-soluble forms of organic matter. Subsequent contact of the oxidized coal components with a suitable inoculum containing a methanogenic microbial community produces methane.

Abstract: A method for in-situ microbial oxygen generation in an underground hydrocarbon containing formation comprises: injecting into the formation an oxygen generating composition comprising thermophilic chlorate reducing micro-organisms, such as bacteria of the genus Archaeoglobus, Geobacillus and/or Thermus, which multiply at a temperature of at least 60° C.

Abstract: Disclosed herein is a method to control distribution of injected microorganisms, nutrients or other chemical treatment fluids from a single injection well to multiple production wells. The sweep efficiency is improved preventing premature production of fluids from production wells with short breakthrough times. This is a useful technique for treatment of subterranean target sites for practice of Microbial Enhanced Oil Recovery and bioremediation.

Abstract: A novel method reduces methane slack when operating a biogas fermenter. When starting up a freshly charged fermenter, the methane portion of the produced biogas is initially so low and the portions of carbon dioxide and nitrogen are so high that the biogas cannot be directly used in a combined heat and power plant. Conventionally, the biogas generated during the startup phase has a small portion of methane that is discharged directly into the atmosphere or is flared off if the methane fraction is larger. The initially produced methane is consequently not used and becomes methane slack. To reduce methane slack, the biogas with the low methane fraction is fed to a gas treatment unit in which non-methane components of the gas mixture are partially separated, and the remaining gas mixture with a higher methane content is returned to the biogas fermenter until the methane fraction is sufficiently high.

Abstract: The invention relates to a method to repair a leak in a geological structure or a construction comprising a geological material, comprising administering a liquid nutrient composition, comprising an energy source for anaerobic bacteria and a multivalent metal ion upstream of the leak allowing micro-organisms present in the structure or construction to ferment the energy source and to grow, thereby increasing biomass in and/or around the leak releasing mineral particles upstream of the leak allowing the mineral particles to migrate to the leak; and allowing the mineral particles to settle in the biomass.

Abstract: A method to increase oil production from an oil well comprising a plurality of production zones is presented, wherein the method selects a production zone, isolates that selected production zone, and reconfigures flow lines to allow direct injection of materials under pressure into the selected production zone. The method determines a treatment pressure based upon soil porosity in the selected zone, injects a brine solution into the selected production zone at the selected pressure, and maintains the selected pressure in the selected production zone for at least 72 hours.

Abstract: A process for producing mineral oil from mineral oil deposits by injecting aqueous flooding media into a mineral oil formation through injection boreholes and withdrawing the mineral oil through production boreholes, wherein the process comprises several cycles of process steps in which oil-mobilizing microorganisms and flooding water are injected alternately into the deposit. Processes in which highly permeable regions of the mineral oil formation are additionally blocked.

Abstract: The present invention describes methods of stimulating the biogenic production of methane in hydrocarbon-bearing formations. The present application provides various stimulants which, when contacted with a hydrocarbon deposit in situ or ex situ, induce or enhance coalbed methane production.

Abstract: Viscoelastic surfactant (VES) gelled aqueous fluids containing water, a VES, an internal breaker, a VES stabilizer, a fluid loss control agent and a viscosity enhancer are useful as treating fluids and particularly as fracturing fluids for subterranean formations. These VES-based fluids have faster and more complete clean-up than polymer-based fracturing fluids. The use of an internal breaker permits ready removal of the unique VES micelle based pseudo-filter cake with several advantages including reducing the typical VES loading and total fluid volume since more VES fluid stays within the fracture, generating a more optimum fracture geometry for enhanced reservoir productivity, and treating reservoirs with permeability above the present VES limit of approximately 400 md to at least 2000 md.

Abstract: Processes relating to the production of methane, carbon dioxide, gaseous and liquid hydrocarbons, and other valuable products from subterranean carbon bearing formations, in-situ, are disclosed. In a preferred embodiment, such production utilizes indigenous and/or non-indigenous microbial consortia that are capable of converting a carbon bearing material such as shale or coal to desired products. In a particularly preferred embodiment there is provided a process for bioconverting a carbon-bearing subterranean formation, wherein the process comprises injecting fluid into a carbon bearing deposit with at least one injection well and removing injected fluid and product from the deposit through at least one production well, and controlling fluid pressure within at least a portion of the deposit by use of the injected fluid, the pressure being controlled such that the fluid pressure within at least a portion of the deposit exceeds the fluid pressure that normally exists in that portion.

Abstract: A slick water fracturing fluid including a brine, an inorganic nitrate, a nitrogen reducing bacteria, a scale inhibitor selected from the group consisting of a polyacrylate polymer, a polyacrylate copolymer, a polyacrylate terpolymer, and mixtures thereof, and a friction reducer, wherein the friction reducer is a polyacrylamide.

Abstract: Systems for enhanced in-situ or perhaps even ex-situ biogenic methane production from hydrocarbon-bearing formations (1) including coal seam, oil shale, coal, coal derivates and the like are presented in embodiments such as but not limited to: increasing and perhaps even selection of microbial populations (2), amending CBM water and other microbe-containing media, diminishing sulfate reduction competition, enhancing organic matter concentrations and generation of biogenic methane (10), universally treating hydrocarbon-bearing formations, and introducing amendments (3) to hydrocarbon-bearing formations.

Abstract: A method to improve the effectiveness of MEOR or bioremediation processes has been disclosed. In this method toxic chemicals accumulated in subterranean sites adjacent to the water injection wells are either dispersed or removed prior to introduction of microbial inocula for enhanced microbial oil recovery or bioremediation of these sites.

Abstract: A method to improve the effectiveness of MEOR or bioremediation processes has been disclosed. In this method toxic chemicals accumulated in subterranean sites adjacent to the water injection wells are either dispersed or removed prior to introduction of microbial inocula for enhanced microbial oil recovery or bioremediation of these sites.

Abstract: A method to improve the effectiveness of MEOR or bioremediation processes has been disclosed. In this method toxic chemicals accumulated in subterranean sites adjacent to the water injection wells are either dispersed or removed prior to introduction of microbial inocula for enhanced microbial oil recovery or bioremediation of these sites.